Current Biology最新文献_第6页

Island-wide removal of honeybees reveals exploitative trophic competition with strongly declining wild bee populations.

IF 8.1 1区生物学

Current Biology Pub Date : 2025-03-14 DOI: 10.1016/j.cub.2025.02.048

Lorenzo Pasquali, Claudia Bruschini, Fulvia Benetello, Marco Bonifacino, Francesca Giannini, Elisa Monterastelli, Marco Penco, Sabrina Pesarini, Vania Salvati, Giulia Simbula, Marta Skowron Volponi, Stefania Smargiassi, Elia van Tongeren, Giorgio Vicari, Alessandro Cini, Leonardo Dapporto

{"title":"Island-wide removal of honeybees reveals exploitative trophic competition with strongly declining wild bee populations.","authors":"Lorenzo Pasquali, Claudia Bruschini, Fulvia Benetello, Marco Bonifacino, Francesca Giannini, Elisa Monterastelli, Marco Penco, Sabrina Pesarini, Vania Salvati, Giulia Simbula, Marta Skowron Volponi, Stefania Smargiassi, Elia van Tongeren, Giorgio Vicari, Alessandro Cini, Leonardo Dapporto","doi":"10.1016/j.cub.2025.02.048","DOIUrl":"https://doi.org/10.1016/j.cub.2025.02.048","url":null,"abstract":"High densities of managed honeybees (Apis mellifera) can threaten wild bees through exploitative competition, thus leading to population declines of the latter. Although reviews have outlined key steps to demonstrate these impacts-measuring resource overlap, changes in wild bee behavior, and population trends-studies that comprehensively address these aspects are virtually absent. We were granted access to the entire protected island of Giannutri (2.6 km2) and to the apiary (18 hives) located there during the early phase of coexistence between honeybees and wild bees. Using the island as an open-air laboratory, we experimentally manipulated honeybee pressure by closing the hives on selected days during the peak of the wild bee foraging period. In the plants most visited by pollinators, even short-term honeybee removals (11 h per day) increased nectar volume (∼60%) and pollen availability (∼30%). In the absence of honeybees, target wild bees (Anthophora dispar and Bombus terrestris) became dominant in the insect-plant visitation network, and the potential apparent competition significantly decreased. Accordingly, both species intensified their foraging activity and increased nectar suction time, a recognized proxy for the quantity of probed nectar, and Bombus terrestris also shortened the time of pollen searching. Transect monitoring revealed an alarming ∼80% decline in both species over 4 years, consistent with honeybee monopolization of floral resources, thus reducing availability for wild pollinators and altering their foraging budget. These findings underscore the risks of introducing high densities of honeybees into protected areas and emphasize the need for rigorous preventive ecological assessments.","PeriodicalId":11359,"journal":{"name":"Current Biology","volume":" ","pages":""},"PeriodicalIF":8.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of pre-dawn arousal in Drosophila by a pair of trissinergic descending neurons of the visual and circadian networks.

IF 8.1 1区生物学

Current Biology Pub Date : 2025-03-14 DOI: 10.1016/j.cub.2025.02.056

Ruihan Jiang, Yue Tian, Xin Yuan, Fang Guo

{"title":"Regulation of pre-dawn arousal in Drosophila by a pair of trissinergic descending neurons of the visual and circadian networks.","authors":"Ruihan Jiang, Yue Tian, Xin Yuan, Fang Guo","doi":"10.1016/j.cub.2025.02.056","DOIUrl":"https://doi.org/10.1016/j.cub.2025.02.056","url":null,"abstract":"Circadian neurons form a complex neural network that generates circadian oscillations. How the circadian neural network transmits circadian signals to other brain regions, thereby regulating the activity patterns in fruit flies, is not well known. Using the FlyWire database, we identified a cluster of descending neurons, DNp27, which is densely connected with key circadian neurons and the visual circuit, projecting extensively across the brain. DNp27 receives excitatory inputs from the circadian neurons DN3s at night and photo-inhibitory signals predominantly during the day, resulting in calcium oscillations that peak in the early morning and dip at dusk. Experimental manipulation of DNp27 revealed its role in activity regulation: artificial activation of DNp27 decreased flies' activity, while ablation or silencing led to an advance in the morning anticipatory peak. Similar alterations in the morning peak were observed following pan-neuronal knockdown of either Trissin or TrissinR, suggesting the involvement of this neuropeptide signaling pathway in DNp27 function. Moreover, neural circuitry and connectivity analyses indicate that DNp27 may regulate circadian neurons via extra-clock electrical oscillators (xCEOs). Lastly, we found that DNp27 modulates arousal thresholds by inhibiting light-responsive activity in the central brain, thereby promoting sleep stability, particularly in the pre-dawn period. Together, these findings suggest that DNp27 plays a crucial role in maintaining stable sleep patterns.","PeriodicalId":11359,"journal":{"name":"Current Biology","volume":" ","pages":""},"PeriodicalIF":8.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nuclear exclusion of condensin I in prophase coordinates mitotic chromosome reorganization to ensure complete sister chromatid resolution.

IF 8.1 1区生物学

Current Biology Pub Date : 2025-03-13 DOI: 10.1016/j.cub.2025.02.047

John K Eykelenboom, Marek Gierliński, Zuojun Yue, Tomoyuki U Tanaka

{"title":"Nuclear exclusion of condensin I in prophase coordinates mitotic chromosome reorganization to ensure complete sister chromatid resolution.","authors":"John K Eykelenboom, Marek Gierliński, Zuojun Yue, Tomoyuki U Tanaka","doi":"10.1016/j.cub.2025.02.047","DOIUrl":"https://doi.org/10.1016/j.cub.2025.02.047","url":null,"abstract":"During early mitosis, chromosomes transition from their unfolded interphase state to the distinct rod-shaped structures characteristic of mitosis. This process allows correct segregation of replicated sister chromatids to the opposite spindle poles during anaphase. Two protein complexes, named condensin I and condensin II, facilitate mitotic chromosome organization. Condensin II is important for achieving sister chromatid separation (resolution), while condensin I is required for chromosome condensation (folding). Although sister chromatid resolution occurs earlier than chromosome folding, it is not yet clear how these events are coordinated through time or whether this is important for correct chromosome segregation. In this study, we tested the hypothesis that temporal control is achieved through differential localization of the two condensin complexes; i.e., while condensin II localizes in the nucleus, condensin I is excluded from the nucleus in interphase and prophase. We engineered the localization of condensin I to the nucleus and monitored sister chromatid resolution and chromosome folding by real-time imaging. We found that localization of condensin I to the nucleus led to precocious chromosome folding during prophase, with similar timing to sister chromatid resolution. Furthermore, this change led to incomplete sister chromatid resolution in prometaphase/metaphase and frequent chromosome missegregation in anaphase, in which most missegregated chromosomes consisted of lagging chromosomes involving both sister chromatids. We conclude that, in a physiological context, the exclusion of condensin I from the nucleus during prophase delays chromosome folding and allows condensin II to complete sister chromatid resolution, which ensures correct chromosome segregation later in mitosis.","PeriodicalId":11359,"journal":{"name":"Current Biology","volume":" ","pages":""},"PeriodicalIF":8.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An accumbal microcircuit for the transition from acute to chronic pain.

IF 8.1 1区生物学

Current Biology Pub Date : 2025-03-13 DOI: 10.1016/j.cub.2025.02.055

Yu-Mei Yu, Sun-Hui Xia, Zheng Xu, Wei-Nan Zhao, Lingzhen Song, Xiangyu Pan, Chao-Chao Zhong, Di Wang, Yi-Hong Gao, Jun-Xia Yang, Peng Wu, Hongxing Zhang, Shuming An, Jun-Li Cao, Hai-Lei Ding

{"title":"An accumbal microcircuit for the transition from acute to chronic pain.","authors":"Yu-Mei Yu, Sun-Hui Xia, Zheng Xu, Wei-Nan Zhao, Lingzhen Song, Xiangyu Pan, Chao-Chao Zhong, Di Wang, Yi-Hong Gao, Jun-Xia Yang, Peng Wu, Hongxing Zhang, Shuming An, Jun-Li Cao, Hai-Lei Ding","doi":"10.1016/j.cub.2025.02.055","DOIUrl":"https://doi.org/10.1016/j.cub.2025.02.055","url":null,"abstract":"Persistent nociceptive inputs arising from peripheral tissues or/and nerve injuries cause maladaptive changes in neurons or neural circuits in the central nervous system, which further confer acute injury into chronic pain transitions (pain chronification) even after the injury is resolved. However, the critical brain regions and their neural mechanisms involved in this transition have not yet been elucidated. Here, we reveal an accumbal microcircuit that is essential for pain chronification. Notably, the increase of neuronal activity in the nucleus accumbens shell (NAcS) in the acute phase (<7 days) and in core (NAcC) in the chronic phase (14-21 days) was detected in a neuropathic pain mouse model. Importantly, we demonstrated that the NAcS neuronal activation in the acute phase of injury was necessary and sufficient for the development of chronic neuropathic pain. This process was mediated by the accumbal dopamine D2 receptor-expressing neuronal microcircuit from NAcS to NAcC. Thus, our findings reveal an accumbal microcircuit mechanism for pain chronification and suggest that the early intervention targeting this microcircuit may provide a therapeutic approach to pain chronification.","PeriodicalId":11359,"journal":{"name":"Current Biology","volume":" ","pages":""},"PeriodicalIF":8.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nonlinear high-activity neuronal excitation enhances odor discrimination.

IF 8.1 1区生物学

Current Biology Pub Date : 2025-03-13 DOI: 10.1016/j.cub.2025.02.034

Julia E Manoim-Wolkovitz, Tal Camchy, Eyal Rozenfeld, Hao-Hsin Chang, Hadas Lerner, Ya-Hui Chou, Ran Darshan, Moshe Parnas

{"title":"Nonlinear high-activity neuronal excitation enhances odor discrimination.","authors":"Julia E Manoim-Wolkovitz, Tal Camchy, Eyal Rozenfeld, Hao-Hsin Chang, Hadas Lerner, Ya-Hui Chou, Ran Darshan, Moshe Parnas","doi":"10.1016/j.cub.2025.02.034","DOIUrl":"https://doi.org/10.1016/j.cub.2025.02.034","url":null,"abstract":"Discrimination between different signals is crucial for animals' survival. Inhibition that suppresses weak neural activity is crucial for pattern decorrelation. Our understanding of alternative mechanics that allow efficient signal classification remains incomplete. We show that Drosophila olfactory receptor neurons (ORNs) have numerous intraglomerular axo-axonal connections mediated by the G protein-coupled receptor (GPCR), muscarinic type B receptor (mAChR-B). Contrary to its usual inhibitory role, mAChR-B participates in ORN excitation. The excitatory effect of mAChR-B only occurs at high ORN firing rates. A computational model demonstrates that nonlinear intraglomerular or global excitation decorrelates the activity patterns of ORNs of different types and improves odor classification and discrimination, while acting in concert with the previously known inhibition. Indeed, knocking down mAChR-B led to increased correlation in odor-induced ORN activity, which was associated with impaired odor discrimination, as shown in behavioral experiments. Furthermore, knockdown (KD) of mAChR-B and the GABAergic GPCR, GABAB-R, has an additive behavioral effect, causing reduced odor discrimination relative to single-KD flies. Together, this study unravels a novel mechanism for neuronal pattern decorrelation, which is based on nonlinear intraglomerular excitation.","PeriodicalId":11359,"journal":{"name":"Current Biology","volume":" ","pages":""},"PeriodicalIF":8.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex-specific neurons instruct sexually dimorphic neurite branching via Netrin signaling in Caenorhabditis elegans.

IF 8.1 1区生物学

Current Biology Pub Date : 2025-03-12 DOI: 10.1016/j.cub.2025.02.050

Dongyoung Kim, HoYong Jin, Da-Hyun Kang, Byunghyuk Kim

{"title":"Sex-specific neurons instruct sexually dimorphic neurite branching via Netrin signaling in Caenorhabditis elegans.","authors":"Dongyoung Kim, HoYong Jin, Da-Hyun Kang, Byunghyuk Kim","doi":"10.1016/j.cub.2025.02.050","DOIUrl":"https://doi.org/10.1016/j.cub.2025.02.050","url":null,"abstract":"Animals often exhibit sexually dimorphic behavior in mating, learning, and decision-making. These sexual dimorphisms arise due to sex differences in the structure and function of neural circuits, but how sexually dimorphic neural circuits are established remains less understood. In the nematode C. elegans, both males and hermaphrodites possess a set of sex-shared neurons with sexually dimorphic features that contribute to the observed sex differences in neural connectivity. Here, we focused on the motor neuron preanal cell body dorsal axon B (PDB) to investigate the molecular mechanism underlying sexually dimorphic neurite branching. The PDB neuron exhibits extensive neurite branches near the cell body in males but not in hermaphrodites. By manipulating the sexual identity of PDB neurons, we discovered that neurite branching is influenced by both cell-autonomous and non-autonomous factors. We found that the UNC-6/Netrin signaling is crucial for the elaborate PDB neurite branching in males. Specifically, UNC-6/Netrin, expressed in a set of male-specific neurons, induces the formation of PDB neurite branches. The cognate receptor UNC-40/deleted in colorectal cancer (DCC), located in the PDB neurites, plays a role in mediating neurite branching in response to the UNC-6/Netrin cue. Furthermore, we show that males with aberrant PDB neurite branches exhibit defects in male mating behavior, particularly in coordinating movements required for successful mating. Our findings provide insights into the establishment of sexually dimorphic neural circuits, demonstrating how an evolutionarily conserved molecular cue and its receptor can be utilized in this process.","PeriodicalId":11359,"journal":{"name":"Current Biology","volume":" ","pages":""},"PeriodicalIF":8.1,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diplonemid protists possess exotic endomembrane machinery, impacting models of membrane trafficking in modern and ancient eukaryotes.

IF 8.1 1区生物学

Current Biology Pub Date : 2025-03-12 DOI: 10.1016/j.cub.2025.02.032

Kristína Záhonová, Julius Lukeš, Joel B Dacks

{"title":"Diplonemid protists possess exotic endomembrane machinery, impacting models of membrane trafficking in modern and ancient eukaryotes.","authors":"Kristína Záhonová, Julius Lukeš, Joel B Dacks","doi":"10.1016/j.cub.2025.02.032","DOIUrl":"https://doi.org/10.1016/j.cub.2025.02.032","url":null,"abstract":"Diplonemids are among the most abundant and species-rich protists in the oceans. Marine heterotrophic flagellates, including diplonemids, have been suggested to play important roles in global biogeochemical cycles. Diplonemids are also the sister taxon of kinetoplastids, home to trypanosomatid parasites of global health importance, and thus are informative about the evolution of kinetoplastid biology. However, the genomic and cellular complement that underpins diplonemids' highly successful lifestyle is underexplored. At the same time, our framework describing cellular processes may not be as broadly applicable as presumed, as it is largely derived from animal and fungal model organisms, a small subset of extant eukaryotic diversity. In addition to uniquely evolved machinery in animals and fungi, there exist components with sporadic (i.e., \"patchy\") distributions across other eukaryotes. A most intriguing subset are components (\"jötnarlogs\") stochastically present in a wide range of eukaryotes but lost in animal and/or fungal models. Such components are considered exotic curiosities but may be relevant to inferences about the complexity of the last eukaryotic common ancestor (LECA) and frameworks of modern cell biology. Here, we use comparative genomics and phylogenetics to comprehensively assess the membrane-trafficking system of diplonemids. They possess several proteins thought of as kinetoplastid specific, as well as an extensive set of patchy proteins, including jötnarlogs. Diplonemids apparently function with endomembrane machinery distinct from existing cell biological models but comparable with other free-living heterotrophic protists, highlighting the importance of including such exotic components when considering different models of ancient eukaryotic genomic complexity and the cell biology of non-opisthokont organisms.","PeriodicalId":11359,"journal":{"name":"Current Biology","volume":" ","pages":""},"PeriodicalIF":8.1,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drosophila complement-like Mcr acts as a wound-induced inflammatory chemoattractant.

IF 8.1 1区生物学

Current Biology Pub Date : 2025-03-12 DOI: 10.1016/j.cub.2025.02.036

Luigi Zechini, Henry Todd, Thibaut Sanchez, Daniel R Tudor, Jennie S Campbell, Edward Antonian, Stephen J Jenkins, Christopher D Lucas, Andrew J Davidson, Jean van den Elsen, Linus J Schumacher, Alessandro Scopelliti, Will Wood

{"title":"Drosophila complement-like Mcr acts as a wound-induced inflammatory chemoattractant.","authors":"Luigi Zechini, Henry Todd, Thibaut Sanchez, Daniel R Tudor, Jennie S Campbell, Edward Antonian, Stephen J Jenkins, Christopher D Lucas, Andrew J Davidson, Jean van den Elsen, Linus J Schumacher, Alessandro Scopelliti, Will Wood","doi":"10.1016/j.cub.2025.02.036","DOIUrl":"https://doi.org/10.1016/j.cub.2025.02.036","url":null,"abstract":"Sterile tissue injury is accompanied by an acute inflammatory response whereby innate immune cells rapidly migrate to the site of injury guided by pro-inflammatory chemotactic damage signals released at the wound. Understanding this immune response is key to improving human health, and recent advances in imaging technology have allowed researchers using different model organisms to observe this inflammatory response in vivo. Over recent decades, offering a unique combination of live time-lapse microscopy and genetics, the fruit fly Drosophila has emerged as a powerful model system to study inflammatory cell migration within a living animal.1,2,3,4 However, we still know relatively little regarding the identity of the earliest signals that drive this immune cell recruitment and the mechanisms by which they act within the complex, in vivo setting of a multicellular organism. Here, we couple the powerful genetics and live imaging of Drosophila with mathematical modeling to identify the fly complement ortholog-macroglobulin complement-related (Mcr)-as an early, wound-induced chemotactic signal responsible for the inflammatory recruitment of immune cells to injury sites in vivo. We show that epithelial-specific knockdown of Mcr suppresses the recruitment of macrophages to wounds and combine predictive mathematical modeling with in vivo genetics to understand macrophage migration dynamics following manipulation of this chemoattractant. We propose a model whereby Mcr operates alongside hydrogen peroxide to ensure a rapid and efficient immune response to damage, uncovering a novel function for this protein that parallels the chemotactic role of the complement component C5a in mammals.","PeriodicalId":11359,"journal":{"name":"Current Biology","volume":" ","pages":""},"PeriodicalIF":8.1,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasma membrane folding enables constant surface area-to-volume ratio in growing mammalian cells.

IF 8.1 1区生物学

Current Biology Pub Date : 2025-03-11 DOI: 10.1016/j.cub.2025.02.051

Weida Wu, Alice R Lam, Kayla Suarez, Grace N Smith, Sarah M Duquette, Jiaquan Yu, David Mankus, Margaret Bisher, Abigail Lytton-Jean, Scott R Manalis, Teemu P Miettinen

{"title":"Plasma membrane folding enables constant surface area-to-volume ratio in growing mammalian cells.","authors":"Weida Wu, Alice R Lam, Kayla Suarez, Grace N Smith, Sarah M Duquette, Jiaquan Yu, David Mankus, Margaret Bisher, Abigail Lytton-Jean, Scott R Manalis, Teemu P Miettinen","doi":"10.1016/j.cub.2025.02.051","DOIUrl":"10.1016/j.cub.2025.02.051","url":null,"abstract":"All cells are subject to geometric constraints, including the surface area-to-volume (SA/V) ratio, which can limit nutrient uptake, maximum cell size, and cell shape changes. Like the SA/V ratio of a sphere, it is generally assumed that the SA/V ratio of cells decreases as cell size increases. However, the structural complexity of the plasma membrane makes studies of the surface area challenging in cells that lack a cell wall. Here, we investigate near-spherical mammalian cells using single-cell measurements of cell mass and plasma membrane proteins and lipids, which allow us to examine the cell size scaling of cell surface components as a proxy for the SA/V ratio. Surprisingly, in various proliferating cell lines, cell surface components scale proportionally with cell size, indicating a nearly constant SA/V ratio as cells grow larger. This behavior is largely independent of the cell-cycle stage and is also observed in quiescent cells, including primary human monocytes. Moreover, the constant SA/V ratio persists when cell size increases excessively during polyploidization. This is enabled by increased plasma membrane folding in larger cells, as verified by electron microscopy. We also observe that specific cell surface proteins and cholesterol can deviate from the proportional size scaling. Overall, maintaining a constant SA/V ratio ensures sufficient plasma membrane area for critical functions such as cell division, nutrient uptake, growth, and deformation across a wide range of cell sizes.","PeriodicalId":11359,"journal":{"name":"Current Biology","volume":" ","pages":""},"PeriodicalIF":8.1,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An evolutionarily ancient transcription factor drives spore morphogenesis in mushroom-forming fungi.

IF 8.1 1区生物学

Current Biology Pub Date : 2025-03-11 DOI: 10.1016/j.cub.2025.02.025

Zhihao Hou, Zsolt Merényi, Yashu Yang, Yan Zhang, Árpád Csernetics, Balázs Bálint, Botond Hegedüs, Csenge Földi, Hongli Wu, Zsolt Kristóffy, Edit Ábrahám, Nikolett Miklovics, Máté Virágh, Xiao-Bin Liu, Nikolett Zsibrita, Zoltán Lipinszki, Ildikó Karcagi, Wei Gao, László G Nagy

{"title":"An evolutionarily ancient transcription factor drives spore morphogenesis in mushroom-forming fungi.","authors":"Zhihao Hou, Zsolt Merényi, Yashu Yang, Yan Zhang, Árpád Csernetics, Balázs Bálint, Botond Hegedüs, Csenge Földi, Hongli Wu, Zsolt Kristóffy, Edit Ábrahám, Nikolett Miklovics, Máté Virágh, Xiao-Bin Liu, Nikolett Zsibrita, Zoltán Lipinszki, Ildikó Karcagi, Wei Gao, László G Nagy","doi":"10.1016/j.cub.2025.02.025","DOIUrl":"10.1016/j.cub.2025.02.025","url":null,"abstract":"Sporulation is the most widespread means of reproduction and dispersal in fungi and, at the same time, an industrially important trait in crop mushrooms. In the Basidiomycota, sexual spores are produced on specialized cells known as basidia, from which they are forcibly discharged with the highest known acceleration in nature. However, the genetics of sporulation remains poorly known. Here, we identify a new, highly conserved transcription factor, sporulation-related regulator 1 (srr1), and systematically address the genetics of spore formation for the first time in the Basidiomycota. We show that Srr1 regulates postmeiotic spore morphogenesis, but not other aspects of fruiting body development or meiosis, and its role is conserved in the phylogenetically distant, but industrially important, Pleurotus spp. (oyster mushrooms). We used RNA sequencing to understand genes directly or indirectly regulated by Srr1 and identified a strongly supported binding motif for the protein. Using an inferred network of putative target genes regulated by Srr1 and comparative genomics, we identified genes lost in secondarily non-ballistosporic taxa, including a novel sporulation-specific chitinase gene. Overall, our study offers systematic insights into the genetics of spore morphogenesis in the Basidiomycota.","PeriodicalId":11359,"journal":{"name":"Current Biology","volume":" ","pages":""},"PeriodicalIF":8.1,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143613951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0