Current Biology最新文献_第10页

A systems model of alternating theta sweeps via firing rate adaptation.

IF 8.1 1区生物学

Current Biology Pub Date : 2025-02-24 Epub Date: 2025-02-10 DOI: 10.1016/j.cub.2024.08.059

Zilong Ji, Tianhao Chu, Si Wu, Neil Burgess

{"title":"A systems model of alternating theta sweeps via firing rate adaptation.","authors":"Zilong Ji, Tianhao Chu, Si Wu, Neil Burgess","doi":"10.1016/j.cub.2024.08.059","DOIUrl":"10.1016/j.cub.2024.08.059","url":null,"abstract":"Place and grid cells provide a neural system for self-location and tend to fire in sequences within each cycle of the hippocampal theta rhythm when rodents run on a linear track. These sequences correspond to the decoded location of the animal sweeping forward from its current location (\"theta sweeps\"). However, recent findings in open-field environments show alternating left-right theta sweeps and propose a circuit for their generation. Here, we present a computational model of this circuit, comprising theta-modulated head-direction cells, conjunctive grid × direction cells, and pure grid cells, based on continuous attractor dynamics, firing rate adaptation, and modulation by the medial-septal theta rhythm. Due to firing rate adaptation, the head-direction ring attractor exhibits left-right sweeps coding for internal direction, providing an input to the grid cell attractor network shifted along the internal direction, via an intermediate layer of conjunctive grid × direction cells, producing left-right sweeps of position by grid cells. Our model explains the empirical findings, including the alignment of internal position and direction sweeps and the dependence of sweep length on grid spacing. It makes predictions for theta-modulated head-direction cells, including relationships between theta phase precession during turning, theta skipping, anticipatory firing, and directional tuning width, several of which we verify in experimental data from anteroventral thalamus. The model also predicts relationships between position and direction sweeps, running speed, and dorsal-ventral location within the entorhinal cortex. Overall, a simple intrinsic mechanism explains the complex theta dynamics of an internal direction signal within the hippocampal formation, with testable predictions.","PeriodicalId":11359,"journal":{"name":"Current Biology","volume":" ","pages":"709-722.e5"},"PeriodicalIF":8.1,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143398635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Avoidable false PSMC population size peaks occur across numerous studies.

IF 8.1 1区生物学

Current Biology Pub Date : 2025-02-24 Epub Date: 2025-02-06 DOI: 10.1016/j.cub.2024.09.028

Leon Hilgers, Shenglin Liu, Axel Jensen, Thomas Brown, Trevor Cousins, Regev Schweiger, Katerina Guschanski, Michael Hiller

{"title":"Avoidable false PSMC population size peaks occur across numerous studies.","authors":"Leon Hilgers, Shenglin Liu, Axel Jensen, Thomas Brown, Trevor Cousins, Regev Schweiger, Katerina Guschanski, Michael Hiller","doi":"10.1016/j.cub.2024.09.028","DOIUrl":"10.1016/j.cub.2024.09.028","url":null,"abstract":"Inferring historical population sizes is key to identifying drivers of ecological and evolutionary change and crucial to predicting the future of species on our rapidly changing planet. The pairwise sequentially Markovian coalescent (PSMC) method provided a revolutionary framework to reconstruct species' demographic histories over millions of years based on the genome sequence of a single individual. Here, we detected and solved a common artifact in PSMC and related methods: recent population peaks followed by population collapses. Combining real and simulated genomes, we show that these peaks do not represent true population dynamics. Instead, ill-set default parameters cause false peaks in our own and published data, which can be avoided by adjusting parameter settings. Furthermore, we show that certain changes in population structure can cause similar patterns. Newer methods, like Beta-PSMC, perform better but do not always avoid this artifact. Our results suggest testing multiple parameters that split the first time window before interpreting recent population peaks followed by collapses and call for the development of robust methods.","PeriodicalId":11359,"journal":{"name":"Current Biology","volume":" ","pages":"927-930.e3"},"PeriodicalIF":8.1,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRMP/UNC-33 maintains neuronal microtubule arrays by promoting individual microtubule rescue.

IF 8.1 1区生物学

Current Biology Pub Date : 2025-02-24 Epub Date: 2025-01-21 DOI: 10.1016/j.cub.2024.12.030

Xing Liang, Regina Agulto, Kelsie Eichel, Caitlin Ann Taylor, Victor Alexander Paat, Huichao Deng, Kassandra Ori-McKenney, Kang Shen

{"title":"CRMP/UNC-33 maintains neuronal microtubule arrays by promoting individual microtubule rescue.","authors":"Xing Liang, Regina Agulto, Kelsie Eichel, Caitlin Ann Taylor, Victor Alexander Paat, Huichao Deng, Kassandra Ori-McKenney, Kang Shen","doi":"10.1016/j.cub.2024.12.030","DOIUrl":"10.1016/j.cub.2024.12.030","url":null,"abstract":"Microtubules (MTs) are intrinsically dynamic polymers. In neurons, staggered individual microtubules form stable, polarized acentrosomal MT arrays spanning the axon and dendrite to support long-distance intracellular transport. How the stability and polarity of these arrays are maintained when individual MTs remain highly dynamic is still an open question. Here, we visualize MT arrays in vivo in C. elegans neurons with single MT resolution. We find that the CRMP family homolog UNC-33 is essential for the stability and polarity of MT arrays in neurites. In unc-33 mutants, MTs exhibit dramatically reduced rescue after catastrophe, develop gaps in coverage, and lose their polarity, leading to trafficking defects. UNC-33 is stably anchored on the cortical cytoskeleton and forms patch-like structures along the dendritic shaft. These discrete and stable UNC-33 patches concentrate free tubulins and correlate with MT rescue sites. In vitro, purified UNC-33 preferentially associates with MT tips and increases MT rescue frequency. Together, we propose that UNC-33 functions as a microtubule-associated protein (MAP) to promote individual MT rescue locally. Through this activity, UNC-33 prevents the loss of individual MTs, thereby maintaining the coverage and polarity of MT arrays throughout the lifetime of neurons.","PeriodicalId":11359,"journal":{"name":"Current Biology","volume":" ","pages":"734-745.e8"},"PeriodicalIF":8.1,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The atypical proteome of mitochondria from mature pollen grains.

IF 8.1 1区生物学

Current Biology Pub Date : 2025-02-24 Epub Date: 2025-01-28 DOI: 10.1016/j.cub.2024.12.037

Clément Boussardon, Matthieu Simon, Chris Carrie, Matthew Fuszard, Etienne H Meyer, Françoise Budar, Olivier Keech

{"title":"The atypical proteome of mitochondria from mature pollen grains.","authors":"Clément Boussardon, Matthieu Simon, Chris Carrie, Matthew Fuszard, Etienne H Meyer, Françoise Budar, Olivier Keech","doi":"10.1016/j.cub.2024.12.037","DOIUrl":"10.1016/j.cub.2024.12.037","url":null,"abstract":"To propagate their genetic material, flowering plants rely on the production of large amounts of pollen grains that are capable of germinating on a compatible stigma. Pollen germination and pollen tube growth are thought to be extremely energy-demanding processes. This raises the question of whether mitochondria from pollen grains are specifically tuned to support this developmental process. To address this question, we isolated mitochondria from both mature pollen and floral buds using the isolation of mitochondria tagged in specific cell-type (IMTACT) strategy and examined their respective proteomes. Strikingly, mitochondria from mature pollen grains have lost many proteins required for genome maintenance, gene expression, and translation. Conversely, a significant accumulation of proteins associated with the tricarboxylic acid (TCA) cycle, the electron transport chain (ETC), and Ca2+ homeostasis was observed. This supports the current model in which pollen requires large quantities of ATP for tube growth but also identifies an unexpected depletion of the gene expression machinery, aligned with the fact that the mitochondrial genome is actively degraded during pollen maturation. Altogether, our results uncover that mitochondria from mature pollen grains are strategically prepared for action by increasing their respiratory capacity and dismantling their gene expression machinery, which raises new questions about the assembly of respiratory complexes in pollen mitochondria, as they rely on the integration of proteins coded by the nuclear and mitochondrial genomes. In addition, the approach described here opens a new range of possibilities for studying mitochondria during pollen development and in pollen-specific mitochondrial events.","PeriodicalId":11359,"journal":{"name":"Current Biology","volume":" ","pages":"776-787.e5"},"PeriodicalIF":8.1,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abscisic acid receptors functionally converge across 500 million years of land plant evolution.

IF 8.1 1区生物学

Current Biology Pub Date : 2025-02-24 Epub Date: 2025-01-31 DOI: 10.1016/j.cub.2024.12.043

Gil Zimran, Michal Shpilman, Eve Hobson, Yasuko Kamisugi, Amichai Baichman-Kass, Hong Zhang, Rafa Ruiz-Partida, María R González-Bermúdez, Matan Azar, Erez Feuer, Maayan Gal, Jorge Lozano-Juste, Jan de Vries, Andrew C Cuming, Assaf Mosquna, Yufei Sun

{"title":"Abscisic acid receptors functionally converge across 500 million years of land plant evolution.","authors":"Gil Zimran, Michal Shpilman, Eve Hobson, Yasuko Kamisugi, Amichai Baichman-Kass, Hong Zhang, Rafa Ruiz-Partida, María R González-Bermúdez, Matan Azar, Erez Feuer, Maayan Gal, Jorge Lozano-Juste, Jan de Vries, Andrew C Cuming, Assaf Mosquna, Yufei Sun","doi":"10.1016/j.cub.2024.12.043","DOIUrl":"10.1016/j.cub.2024.12.043","url":null,"abstract":"Abscisic acid (ABA) functions as a central regulator of dehydration responses in land plants. As such, ABA signaling was pivotal in facilitating the colonization of terrestrial habitats. The conserved ABA signal transduction module consists of 2C-type protein phosphatases (PP2Cs) and their ABA-triggered inhibitors, PYRABACTIN RESISTANCE 1-like proteins (PYLs). Recent evidence indicates that ABA perception emerged from a latent signaling pathway involving a constitutively PP2C-inhibiting PYL homolog. Consequently, ancestral ABA receptors exerted high background signaling, limiting the dynamic range of ABA-dependent signaling. In angiosperms, ABA receptor families are characteristically large and diverse and include a clade-specific subgroup whose members form homodimers, thereby assuming strict ABA dependency. Here, we show that ABA receptors in mosses originate from an independent expansion, giving rise to three subfamilies. Yeast two-hybrid and in vitro PP2C-inhibition assays indicate that moss PYLs feature low basal activities. However, size-exclusion chromatography and additional lines of evidence suggest that moss PYLs are predominantly monomeric. A combination of mutational analysis with biochemical and physiological assays reveals that the reduced basal activities of moss PYLs are achieved through unique sets of amino acid variations. Finally, introducing causal variations to dimeric receptors dramatically compromises their ABA responsiveness, suggesting that the two evolutionary trajectories are mutually exclusive. Hence, mosses appear to have evolved a parallel mechanism to mitigate the ancestrally high background signal of the core ABA perception apparatus. This convergence highlights the shared imperative of expanding the amplitude of a central, highly adaptive signaling pathway.","PeriodicalId":11359,"journal":{"name":"Current Biology","volume":" ","pages":"818-830.e4"},"PeriodicalIF":8.1,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complexity of the lichen symbiosis revealed by metagenome and transcriptome analysis of Xanthoria parietina.

IF 8.1 1区生物学

Current Biology Pub Date : 2025-02-24 Epub Date: 2025-01-30 DOI: 10.1016/j.cub.2024.12.041

Gulnara Tagirdzhanova, Klara Scharnagl, Neha Sahu, Xia Yan, Angus Bucknell, Adam R Bentham, Clara Jégousse, Sandra Lorena Ament-Velásquez, Ioana Onuț-Brännström, Hanna Johannesson, Dan MacLean, Nicholas J Talbot

{"title":"Complexity of the lichen symbiosis revealed by metagenome and transcriptome analysis of Xanthoria parietina.","authors":"Gulnara Tagirdzhanova, Klara Scharnagl, Neha Sahu, Xia Yan, Angus Bucknell, Adam R Bentham, Clara Jégousse, Sandra Lorena Ament-Velásquez, Ioana Onuț-Brännström, Hanna Johannesson, Dan MacLean, Nicholas J Talbot","doi":"10.1016/j.cub.2024.12.041","DOIUrl":"10.1016/j.cub.2024.12.041","url":null,"abstract":"Lichens are composite, symbiotic associations of fungi, algae, and bacteria that result in large, anatomically complex organisms adapted to many of the world's most challenging environments. How such intricate, self-replicating lichen architectures develop from simple microbial components remains unknown because of their recalcitrance to experimental manipulation. Here, we report a metagenomic and metatranscriptomic analysis of the lichen Xanthoria parietina at different developmental stages. We identified 168 genomes of symbionts and lichen-associated microbes across the sampled thalli, including representatives of green algae, three different classes of fungi, and 14 bacterial phyla. By analyzing the occurrence of individual species across lichen thalli from diverse environments, we defined both substrate-specific and core microbial components of the lichen. Metatranscriptomic analysis of the principal fungal symbiont from three different developmental stages of a lichen, compared with axenically grown fungus, revealed differential gene expression profiles indicative of lichen-specific transporter functions, specific cell signaling, transcriptional regulation, and secondary metabolic capacity. Putative immunity-related proteins and lichen-specific structurally conserved secreted proteins resembling fungal pathogen effectors were also identified, consistent with a role for immunity modulation in lichen morphogenesis.","PeriodicalId":11359,"journal":{"name":"Current Biology","volume":" ","pages":"799-817.e5"},"PeriodicalIF":8.1,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vestibular system: A revolution in understanding a neglected sensory system.

IF 8.1 1区生物学

Current Biology Pub Date : 2025-02-24 DOI: 10.1016/j.cub.2025.01.011

James R Lackner

引用次数: 0

Chemosensation: Dynamic CO₂ sensing guides parasitic nematode navigation.

IF 8.1 1区生物学

Current Biology Pub Date : 2025-02-24 DOI: 10.1016/j.cub.2025.01.020

Koustubh M Vaze, X Z Shawn Xu

引用次数: 0

Cross-modal cortical circuit for sound sensitivity in neuropathic pain.

IF 8.1 1区生物学

Current Biology Pub Date : 2025-02-24 Epub Date: 2025-01-30 DOI: 10.1016/j.cub.2024.12.044

Yunfeng Mao, Mingjun Zhang, Xiaoqi Peng, Yi Liu, Yehao Liu, Qianhui Xia, Bin Luo, Lin Chen, Zhi Zhang, Yuanyin Wang, Haitao Wang

{"title":"Cross-modal cortical circuit for sound sensitivity in neuropathic pain.","authors":"Yunfeng Mao, Mingjun Zhang, Xiaoqi Peng, Yi Liu, Yehao Liu, Qianhui Xia, Bin Luo, Lin Chen, Zhi Zhang, Yuanyin Wang, Haitao Wang","doi":"10.1016/j.cub.2024.12.044","DOIUrl":"10.1016/j.cub.2024.12.044","url":null,"abstract":"Hyperacusis, exaggerated sensitivity to sound, frequently accompanies chronic pain in humans, suggesting interactions between different sensory systems in the brain. However, the neural mechanisms underlying this comorbidity remain largely unexplored. In this study, behavioral tests measuring sound-evoked pupil dilation and reaction times to lick water following auditory stimuli showed hyperacusis-like behaviors in neuropathic pain model mice. Through viral tracing, fiber photometry, and multi-electrode recordings, we identified glutamatergic projections from primary somatosensory cortex (S1HLGlu) to the auditory cortex (ACx) that participate in amplifying sound-evoked neuronal activity following spared nerve injury in the hindlimb. Chemo- or optogenetic manipulation and electrophysiology recordings confirmed that the S1HLGlu → ACx pathway is essential for this heightened response to sound. Specifically, activating this pathway intensified glutamatergic neuronal activity in the ACx and induced hyperacusis-like behaviors, while chemogenetic suppression reversed these effects in neuropathic pain model mice. These findings illustrate the mechanism by which central gain increases in the ACx of neuropathic pain mice, improving our understanding of cross-modal sensory system interactions and suggesting circuit pathway targets for developing interventions to treat pain-associated hyperacusis in clinic.","PeriodicalId":11359,"journal":{"name":"Current Biology","volume":" ","pages":"831-842.e5"},"PeriodicalIF":8.1,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eye saccades align optic flow with retinal specializations during object pursuit in freely moving ferrets.

IF 8.1 1区生物学

Current Biology Pub Date : 2025-02-24 Epub Date: 2025-02-04 DOI: 10.1016/j.cub.2024.12.032

Damian J Wallace, Kay-Michael Voit, Daniela Martin Machado, Mohammadreza Bahadorian, Juergen Sawinski, David S Greenberg, Paul Stahr, Carl D Holmgren, Giacomo Bassetto, Federica B Rosselli, Aneta Koseska, David Fitzpatrick, Jason N D Kerr

{"title":"Eye saccades align optic flow with retinal specializations during object pursuit in freely moving ferrets.","authors":"Damian J Wallace, Kay-Michael Voit, Daniela Martin Machado, Mohammadreza Bahadorian, Juergen Sawinski, David S Greenberg, Paul Stahr, Carl D Holmgren, Giacomo Bassetto, Federica B Rosselli, Aneta Koseska, David Fitzpatrick, Jason N D Kerr","doi":"10.1016/j.cub.2024.12.032","DOIUrl":"10.1016/j.cub.2024.12.032","url":null,"abstract":"During prey pursuit, how eye rotations, such as saccades, enable continuous tracking of erratically moving targets while enabling an animal to navigate through the environment is unknown. To better understand this, we measured head and eye rotations in freely running ferrets during pursuit behavior. By also tracking the target and all environmental features, we reconstructed the animal's visual fields and their relationship to retinal structures. In the reconstructed visual fields, the target position clustered on and around the high-acuity retinal area location, the area centralis, and surprisingly, this cluster was not significantly shifted by digital removal of either eye saccades, exclusively elicited when the ferrets made turns, or head rotations that were tightly synchronized with the saccades. Here, we show that, while the saccades did not fixate the moving target with the area centralis, they instead aligned the area centralis with the intended direction of travel. This also aligned the area centralis with features of the optic flow pattern, such as flow direction and focus of expansion, used for navigation by many species. While saccades initially rotated the eyes in the same direction as the head turn, saccades were followed by eye rotations countering the ongoing head rotation, which reduced image blur and limited information loss across the visual field during head turns. As we measured the same head and eye rotational relationship in freely moving tree shrews, rats, and mice, we suggest that these saccades and counter-rotations are a generalized mechanism enabling mammals to navigate complex environments during pursuit.","PeriodicalId":11359,"journal":{"name":"Current Biology","volume":" ","pages":"761-775.e10"},"PeriodicalIF":8.1,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0