Domestic animal endocrinology最新文献

{"title":"Correlation of Anti-Müllerian hormone serum concentration measured in proestrus and estrus with the litter size as a fertility marker in bitches","authors":"Theresa Hornberger ,&nbsp;Sven Reese ,&nbsp;Klaus Perbandt ,&nbsp;Andrea Meyer-Lindenberg ,&nbsp;Beate Walter","doi":"10.1016/j.domaniend.2024.106897","DOIUrl":"10.1016/j.domaniend.2024.106897","url":null,"abstract":"<div><div>The use of the anti-Müllerian hormone (AMH) serum concentration as a fertility marker has been shown in cows, sheep and mares and has been indicated in one study in female dogs. The aim of this study was to investigate the connection between the AMH serum concentration, taken at two defined time points during the bitch`s heat, and the litter size to investigate whether AMH can be used as a practical measurement for the individual breeding bitch to predict litter size. The study was carried out on 27 healthy female dogs presented for pre-breeding examination, considering all previously known influencing factors on AMH in the bitch such as age, body weight and estrous cycle phase at the time the sample was taken. Due to the AMH increase in early proestrus and its drop around ovulation, AMH was measured in blood samples taken within the first three days of heat (AMH1) and near ovulation (AMH2) with AMH1 being significantly higher than AMH 2 (p &lt; 0.001). There was a highly significant negative correlation of body weight and AMH at both sampling times (p &lt; 0.001). There were no significant results when the dogs were paired and grouped according to high or low AMH concentrations, but a significant effect of AMH serum concentration on litter size was found in the multifactorial analysis when the dogs were matched according to their body weight (AMH1: p = 0.022; AMH2: p = 0.030). In conclusion, a significant effect of the AMH concentration and the litter size among female dogs with matching body weight could be found, but a much larger sample collection is needed to evaluate reference intervals for AMH for bitches of different weight to predict the fertility of an individual bitch in the future.</div></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"91 ","pages":"Article 106897"},"PeriodicalIF":1.9,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The structure of the porcine uterine-conceptus interface is associated with gestational day, fetal size and sex","authors":"Tailine Rayane Lima Reis ,&nbsp;João Vitor Lopes-Ferreira ,&nbsp;Karen Beatriz Guerra ,&nbsp;Luana Alice Boggione da Silva ,&nbsp;Claire Stenhouse ,&nbsp;Cheryl Joy Ashworth ,&nbsp;Enrrico Bloise ,&nbsp;Hélio Chiarini-Garcia ,&nbsp;Fernanda Radicchi Campos Lobato de Almeida","doi":"10.1016/j.domaniend.2024.106895","DOIUrl":"10.1016/j.domaniend.2024.106895","url":null,"abstract":"<div><div>This study aimed to characterize histological changes of the maternal-conceptus interface in feto-placental units associated with fetal weight and sex throughout pregnancy. Pregnant Large-White X Landrace gilts(n=18) were euthanized and hysterectomized on gestational days[GDs] 30(n=3), 45(n=5), 60(n=5), and 90(n=5). Intact cross-sections of fetoplacental interface associated with the lightest[LW] and normally-grown[NW] littermates were collected on GD30(n=4 per size). On GDs 45, 60 and 90, interactions between fetal size and sex were investigated in light-weight males[LWM] and females[LWF]; normal-weight males[NWM] and females[NWF] (n=4/group/GD). Fetal weight did not affect the endometrium composition, including relative proportion of glandular epithelium, blood vessels, and connective tissue. Feto-placental units from LW embryos tended to have longer chorioallantoic fold length on GD30(P=0.06). On GD45, higher proportion of larger endometrial glands was observed in NWM, and taller trophoblastic epithelium in NW conceptuses, regardless of sex(P&lt;0.05). NWF presented the greatest proportion of subluminal endometrial epithelial blood vessels(P&lt;0.05). On GD60, more blood vessels were present at the folds’ base in males feto-placental units, whereas taller trophoblastic epithelium were present in NWF fetuses’ feto-placental units(P&lt;0.05). Feto-placental units’ morphological composition throughout gestation in NW and LW conceptuses revealed that fold length was higher as early as GD30, with no further increase up to GD90 in LW conceptuses(P&gt;0.05). Increased proportion of glandular epithelium was observed in LW conceptuses; the highest percentage present on GD90(P&lt;0.05). Collectively, we demonstrated that fetal weight and sex influence the morphological structure of feto-placental units from as early as GD30, suggesting potential differences in the ability for nutrient transport.</div></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"90 ","pages":"Article 106895"},"PeriodicalIF":1.9,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142699781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution and sequence analysis of the melanocortin 2 receptor in horses and ponies","authors":"Christina M. Cash,&nbsp;Melody A. de Laat","doi":"10.1016/j.domaniend.2024.106896","DOIUrl":"10.1016/j.domaniend.2024.106896","url":null,"abstract":"<div><div>The melanocortin 2 receptor (MC2R) has relevance to equine pituitary pars intermedia dysfunction (PPID), as it is the primary binding site for ACTH, which circulates at elevated concentrations in animals affected by PPID. Despite this, little is known about MC2R in equine species. The overall aim of this investigation was to determine MC2R mRNA expression in tissues relevant to PPID in healthy horses and to examine the MC2R gene sequence in a cohort of horses and ponies with and without PPID. The study found that the MC2R gene was expressed in both adrenal and pituitary gland tissues as reported in other mammalian species. However, no expression was seen in adipose or skin tissue. An investigation of the tissue distribution and functionality of the MC2R in individuals with PPID is now recommended. Then, we investigated the coding regions (exons) of the equine MC2R gene for variations associated with PPID in a limited number of animals. This was performed using a hybridised gene capture and next generation sequencing method which found a 100% pairwise identity between all 28 individuals in the study, and with the reference genome sequence. This preliminary study found no evidence of major genetic variations in the coding region of the MC2R gene associated with PPID, though variants affecting expression may occur in the introns or remain unidentified within the exons of the gene and studies on a larger scale are required.</div></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"90 ","pages":"Article 106896"},"PeriodicalIF":1.9,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142699778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-term clinical and biochemical responses following treatment with dapagliflozin or ertugliflozin in horses with hyperinsulinemia: A retrospective case series","authors":"Tania Sundra ,&nbsp;Edd Knowles ,&nbsp;David Rendle ,&nbsp;Erin Kelty ,&nbsp;Guy Lester ,&nbsp;Gabriele Rossi","doi":"10.1016/j.domaniend.2024.106894","DOIUrl":"10.1016/j.domaniend.2024.106894","url":null,"abstract":"<div><div>The metabolic and lipid profiles of horses treated with sodium-glucose cotransporter 2 inhibitors are not well understood. This retrospective study evaluated blood parameters in hyperinsulinemic horses treated with either ertugliflozin (0.05 mg/kg) or dapagliflozin (0.02 mg/kg) orally once daily. Blood samples were collected at baseline (day 0) and after 7 and/or 30 days of treatment. Statistical analyses were conducted using Wilcoxon signed-rank, Mann-Whitney and Spearman's rank correlation tests. Thirty-four horses received dapagliflozin and 24 received ertugliflozin. Significant (p&lt;0.05) within-horse changes between day 0 and day 30 included [median, inter-quartile range (IQR)]: basal serum [Insulin] (uU/ml) reduced 170 (92-280) to 28.7 (14.5-90); [triglycerides] (mmol/l) increased 0.5 (0.3-0.6) to 1.0 (0.6-1.56), [β-hydroxybutyrate] (umol/l) increased 0.22 (0.17-2.7) to 0.30 (0.24-0.35); [total cholesterol] (mmol/l) increased 2.36 (2-2.6) to 2.84 (2.4-3.7); and GGT (IU/ml) increased 21 (16-32) to 25 (18-38). As a percentage of total serum lipids, high-density lipoprotein (HDL) reduced 52.4 % (47.9 %-61.0 %) to 50 % (41 %-54.8 %) and very-low density lipoprotein (VLDL) increased 10.4 % (6.4 %-14.4 %) to 12.3 % (9.9 %-16.8 %) (all p&lt;0.05). Differences between ertugliflozin and dapagliflozin groups were not significant in any of these parameters at days 0, 7 or 30. At day 30, 10/48 (21 %) cases had [triglycerides] &gt; 2.0 mmol/l (maximum = 10.8mmol/l). Day 30 [triglyceride] correlated with day 0: basal insulin (rho=0.47); [triglyceride] (rho=0.42); %VLDL (rho=0.34) day 30: [total cholesterol] (rho=0.67), %HDL (rho=-0.432) and %VLDL (rho=0.708). Our findings suggest that SGLT2 inhibitors induce minor changes in lipid profiles, with occasional cases of marked hypertriglyceridemia, and that dapagliflozin and ertugliflozin exhibit similar biochemical effects.</div></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"90 ","pages":"Article 106894"},"PeriodicalIF":1.9,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142699780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of short-term dopamine reduction on insulin sensitivity and post-prandial insulin and glucose responses in Standardbred horses","authors":"Nicolas C. Galinelli ,&nbsp;Nicholas J. Bamford ,&nbsp;Madison L. Erdody ,&nbsp;Tobias Warnken ,&nbsp;Melody A. de Laat ,&nbsp;Martin N. Sillence ,&nbsp;Patricia A. Harris ,&nbsp;Simon R. Bailey","doi":"10.1016/j.domaniend.2024.106893","DOIUrl":"10.1016/j.domaniend.2024.106893","url":null,"abstract":"<div><div>The role of dopamine in the regulation of insulin secretion in horses is poorly understood and requires further investigation. Pituitary pars intermedia dysfunction (PPID) is associated with decreased activity of dopaminergic neurons which normally suppress peptide hormone secretion from the pituitary pars intermedia. A high proportion of horses with PPID also have insulin dysregulation (ID), characterised by post-prandial hyperinsulinaemia and/or tissue insulin resistance, which are risk factors for the development of laminitis. The aim of this study was to investigate the effects of alpha-methyl-para-tyrosine (AMPT), a tyrosine hydroxylase inhibitor that reduces dopamine production, on insulin sensitivity and the post-prandial insulin response to a glucose-containing meal. Six healthy Standardbred horses were enrolled in a placebo-controlled randomised crossover study, in which one dose of AMPT (40 mg/kg BW) or placebo was administered orally, prior to performing an in-feed oral glucose test (OGT) and a frequently sampled intravenous glucose tolerance test (FSIGTT). Dopamine reduction by AMPT was confirmed by an increase in plasma prolactin concentration and the lack of post-prandial increase in plasma dopamine concentration compared to placebo. Post-prandial insulin responses, both peak and AUCi, were increased after AMPT compared to placebo (<em>P</em>=0.048 and <em>P</em>=0.005, respectively), without affecting blood glucose concentrations. However, one dose of AMPT did not appear to affect tissue sensitivity as assessed by the FSIGTT. This study confirmed that dopamine plays a role in the regulation of insulin secretion in horses, as it does in other species, whereby the post-prandial release of dopamine into the circulation may inhibit pancreatic insulin secretion. Further studies are required to evaluate different dosing protocols for AMPT, and to further investigate the links between PPID, ID and laminitis risk in horses.</div></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"90 ","pages":"Article 106893"},"PeriodicalIF":1.9,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of rumen metabolite butyric acid on bovine skeletal muscle satellite cells proliferation, apoptosis and transcriptional states during myogenic differentiation","authors":"Xiao-Wei Wang ,&nbsp;Yan-Ling Ding ,&nbsp;Cheng-Long Li ,&nbsp;Qing Ma ,&nbsp;Yuan-Gang Shi ,&nbsp;George E Liu ,&nbsp;Cong-Jun Li ,&nbsp;Xiao-Long Kang","doi":"10.1016/j.domaniend.2024.106892","DOIUrl":"10.1016/j.domaniend.2024.106892","url":null,"abstract":"<div><div>Butyric acid, a pivotal short-chain fatty acid in rumen digestion, profoundly influences animal digestive and locomotor systems. Extensive research indicates its direct or indirect involvement in the growth and development of muscle and fat cells. However, the impact of butyric acid on the proliferation and differentiation of bovine skeletal muscle satellite cells (SMSCs) remains unclear. This study aimed to elucidate the effects of butyrate on SMSCs proliferation and differentiation. After isolating, SMSCs were subjected to varying concentrations of sodium butyrate (NaB) during the proliferation and differentiation stages. Optimal treatment conditions (1 mM NaB for 2 days) were determined based on proliferative force, cell viability, and mRNA expression of proliferation and differentiation marker genes. Transcriptome sequencing was employed to screen for differential gene expression between 1 mM NaB-treated and untreated groups during SMSCs differentiation. Results indicated that lower NaB concentrations (≤1.0 mM) inhibited proliferation while promoting differentiation and apoptosis after a 2-day treatment. Conversely, higher NaB concentrations (≥2.0 mM) suppressed proliferation and differentiation and induced apoptosis. Transcriptome sequencing revealed differential expression of genes(<em>ND1, ND3, CYTB, COX2, ATP6, MYOZ2, MYOZ3, MYBPC1</em> and <em>ATP6V0A4</em>,etc.) were associated with SMSCs differentiation and energy metabolism, enriching pathways such as Oxidative phosphorylation, MAPK, and Wnt signaling. These findings offer valuable insights into the molecular mechanisms underlying butyrate regulation of bovine SMSCs proliferation and differentiation, as well as muscle fiber type conversion in the future study.</div></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"90 ","pages":"Article 106892"},"PeriodicalIF":1.9,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and metabolic effects of short-term dopamine reduction in healthy horses using a tyrosine hydroxylase inhibitor (alpha-methyl-para-tyrosine)","authors":"Nicolas C. Galinelli ,&nbsp;Nicholas J. Bamford ,&nbsp;Madison L. Erdody ,&nbsp;Tobias Warnken ,&nbsp;Melody A. de Laat ,&nbsp;Martin N. Sillence ,&nbsp;Patricia A. Harris ,&nbsp;Simon R. Bailey","doi":"10.1016/j.domaniend.2024.106891","DOIUrl":"10.1016/j.domaniend.2024.106891","url":null,"abstract":"<div><div>Alpha-methyl-para-tyrosine (AMPT) is a reversible inhibitor of tyrosine hydroxylase, the rate-limiting enzyme in catecholamine synthesis. This study aimed to determine whether AMPT could reduce dopamine concentrations in horses. Six healthy adult Standardbred geldings were administered AMPT (40 mg/kg BW, orally) or placebo in a randomised crossover study design. Clinical examination findings were recorded, and blood samples were collected for up to 6 h after administration of AMPT or placebo, for measurement of blood glucose, plasma ACTH and cortisol concentrations, and plasma metabolomic analysis. Plasma prolactin concentration was determined as a proxy index of central dopamine reduction. No adverse clinical effects were detected after oral administration of AMPT, with heart rate, mean arterial pressure and blood glucose concentration not differing between AMPT treatment or placebo. Plasma prolactin concentration peaked 1 h after AMPT administration before returning to baseline at 2 h (for five horses) or 6 h (for one horse). Metabolomic analysis demonstrated a reduction in plasma dopamine (0.72-fold change; <em>P</em>=0.016) 1 h after AMPT treatment. Plasma ACTH and cortisol concentrations were not different between AMPT and placebo over time. A few metabolites associated with ketogenesis were increased, and certain amino acids decreased, at 1 h compared with baseline, for both AMPT treatment and placebo. Therefore, AMPT was effective in reducing both central and circulating dopamine concentrations in healthy horses following a single oral dose. Further pharmacokinetic and pharmacodynamic studies are warranted to optimise the dose and duration of AMPT treatment to achieve longer-term dopamine reduction. Plasma metabolomic findings suggested an interruption to energy flux at the time of sample collection, which may be relevant to nutritional studies in horses and warrants further investigation.</div></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"90 ","pages":"Article 106891"},"PeriodicalIF":1.9,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving survival and growth of caprine preantral follicles cultured in medium commonly used for MSC: Role of oxidative stress regulation and epigenetic changes","authors":"Ana F.B. Silva ,&nbsp;Laritza F. Lima ,&nbsp;Anna C.A. Ferreira ,&nbsp;Ariclécio C. Oliveira ,&nbsp;Napoleão M.A. Neto ,&nbsp;Benner G. Alves ,&nbsp;Ana P.R. Rodrigues ,&nbsp;Eduardo L. Gastal ,&nbsp;Vilceu Bordignon ,&nbsp;José R. Figueiredo","doi":"10.1016/j.domaniend.2024.106890","DOIUrl":"10.1016/j.domaniend.2024.106890","url":null,"abstract":"<div><div>This study evaluated the efficiency of <em>in vitro</em> culture of preantral follicles (PAF) in a commonly used medium for mesenchymal stem cell (MSC) culture. Parameters assessed included follicle survival, growth, stromal cell density, levels of reduced thiols and reactive oxygen species, epigenetic changes, cell apoptosis, and mRNA abundance. Caprine ovarian tissues were cultured for 1 or 7 days in either PAF or MSC-common media, with uncultured tissues serving as controls. The MSC medium exhibited increased follicular survival and growth and remodeled stromal density potentially through the regulation of oxidative stress and epigenetic changes compared to the PAF medium. In conclusion, our results highlight the importance of the MSC medium in enhancing follicular survival and growth, changing the stromal cell density, as well as in regulating the medium oxidative stress and epigenetic changes during the <em>in vitro</em> culture of caprine PAF.</div></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"90 ","pages":"Article 106890"},"PeriodicalIF":1.9,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142375251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of circulating miRNAs in mares approaching parturition","authors":"Mio Kikuchi ,&nbsp;Harutaka Murase ,&nbsp;Kenichi Urata ,&nbsp;Taichiro Ishige ,&nbsp;Shun-ichi Nagata ,&nbsp;Teruaki Tozaki ,&nbsp;Hironaga Kakoi ,&nbsp;Toshina Ishiguro-Oonuma ,&nbsp;Keiichiro Kizaki","doi":"10.1016/j.domaniend.2024.106879","DOIUrl":"10.1016/j.domaniend.2024.106879","url":null,"abstract":"<div><p>Circulating microRNAs (miRNAs) are stable in body fluids and can serve as biomarkers for various diseases and physiological states. Although pregnancy˗related miRNAs have been identified in various mammals, studies on parturition˗related circulating miRNAs in mares are limited. Therefore, this study aimed to identify parturition˗related miRNAs and examine their potential applications in the prediction of parturition date. miRNAs were extracted from the plasma of Thoroughbred mares 30 days (295–326 days pregnant) and 5 (323–352 days pregnant) – 0 (328–357 days pregnant) days before parturition, followed by small RNA sequencing (small RNA˗seq) and reverse transcription quantitative PCR (RT˗qPCR). Additionally, we measured plasma progestin concentrations in mares using an enzyme-linked immunosorbent assay. Small RNA˗seq data indicated that 18 miRNAs were affected by parturition proximity. Among the 18 miRNAs, two novel miRNAs and three known miRNAs (miR˗361˗3p, miR˗483, and miR˗99a) showed significant changes at 5–0 days before parturition compared with that at 30 days to parturition. Plasma progestin concentrations were higher at 5–3 days to parturition than at 30 days to parturition, and then decreased on the day of parturition. Conclusively, this study provides basic knowledge of parturition˗related circulating miRNAs in mares, and identifies miRNAs that could potentially be used as biomarkers to predict parturition in mares.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"89 ","pages":"Article 106879"},"PeriodicalIF":1.9,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0739724024000420/pdfft?md5=ff347d9207a7c1d574807dc426ef3878&pid=1-s2.0-S0739724024000420-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142076984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of quercetin on autophagy and apoptosis induced by a high concentration of CuSO4 in porcine ovarian granulosa cells","authors":"Nannan Qi ,&nbsp;Binbin Wang ,&nbsp;Wenwen Xing ,&nbsp;Mengxuan Li ,&nbsp;Jiying Liu","doi":"10.1016/j.domaniend.2024.106881","DOIUrl":"10.1016/j.domaniend.2024.106881","url":null,"abstract":"<div><p>Copper is a vital micronutrient necessary for the maintenance of physiological functions. However, excessive amounts can lead to organ damage. Porcine ovarian granulosa cells are damaged by a high concentration of CuSO₄, which can reduce the reproductive capacity of sows. Quercetin has shown remarkable efficacy in mitigating the harmful effects of heavy metals. Therefore, the aim of this study was to investigate the effects of a high concentration of CuSO₄ on autophagy and apoptosis in porcine ovarian granulosa cells and to explore whether quercetin can counteract these toxic effect. Cell morphology, and the mRNA expression levels of autophagy-related genes (<em>LC3-Ⅰ, ATG5, ATG7, ATG12, Beclin1, mTOR, LC3-Ⅱ</em> and <em>P62</em>) were significantly changed upon treatment with 200 and 400 µM CuSO₄. Treatment with 200 µM CuSO₄ increased expression of P62 protein (<em>P</em>&lt;0.05), promoted LC3-Ⅰ to LC3-Ⅱ conversion (<em>P</em>&lt;0.05), and reduced PINK1 protein expression and the ATP content (<em>P</em>&lt;0.05). In addition, expression of Caspase3 protein was increased and TUNEL staining indicated that the number of apoptotic cells was increased. However, co-treatment with 10 µM quercetin significantly decreased expression of P62 and conversion of LC3-Ⅰ to LC3-Ⅱ. Furthermore, flow cytometric analysis revealed that addition of 10 µM quercetin significantly reduced apoptosis induced by a high concentration of CuSO₄. In summary, the results indicate that a high concentration of CuSO₄ can trigger mitochondrial and autophagy dysfunction, activate mitochondrial apoptosis pathway, and exert cytotoxic effects. Quercetin can mitigate autophagy dysfunction, enhance autophagic processes, and alleviate apoptosis.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"90 ","pages":"Article 106881"},"PeriodicalIF":1.9,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142097589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}