Domestic animal endocrinology最新文献

{"title":"Spatial local expressions of kisspeptin in the uterus and uterine tubes and its relationship to the reproductive potential in goats","authors":"Haney Samir ,&nbsp;Ahmed Elfadadny ,&nbsp;Faten Radwan ,&nbsp;Hossam R. El-Sherbiny ,&nbsp;Ayman A. Swelum ,&nbsp;Wael A. Khalil ,&nbsp;Gen Watanabe","doi":"10.1016/j.domaniend.2024.106850","DOIUrl":"10.1016/j.domaniend.2024.106850","url":null,"abstract":"<div><p>Kisspeptins are neuropeptides encoded by the <em>Kiss1</em> gene that was discovered as a metastasis suppressor gene in melanoma and breast cancer. Kisspeptin has pivotal functions for gonadotropin-releasing hormone secretion and plays integrated roles in the hypothalamic-pituitary-gonadal axis. However, little is known about the peripheral expression of kisspeptin in ruminants, especially in the female reproductive tract. Here, the objectives of the current study were to investigate the spatial localization of kisspeptin and mRNA expression of Kiss1 and its receptor (Kiss1r) in the fallopian tubes (FT) and uterus of goats at varied reproductive activity (cyclic versus true anoestrous goats, n=6, each). Specimens of the uterus and FT were collected and fixed using paraformaldehyde to investigate the localizations of kisspeptin in the selected tissues by immunohistochemistry. Another set of samples was snape-frozen to identify the expressions of mRNAs encoding Kiss1 and Kiss1r using real-time PCR. Results revealed immunolocalizations of kisspeptin in the uterus and the FT. The staining of kisspeptin was found mainly in the mucosal epithelium of the uterus the FT, and the endometrial glands. Very intense staining of kisspeptin was found in the uterine and FT specimens in the true anoestrous goats compared to that in cyclic ones. The expression of mRNA encoding <em>Kiss1</em> gene was significantly higher in the uterine specimen of cyclic goats (1.00±0.09) compared to that in the true anoestrous goats (0.62±0.08) (P ˂0.05), while the expression of mRNA encoding <em>Kiss1r</em> was significantly (P ˂0.001) higher in the uterine tissues of true anoestrous goats (1.78±0.17) compared to that in cyclic ones (1.00±0.11). In conclusion, immunohistochemical localization of kisspeptin and the expression of mRNA encoding <em>Kiss1/Kiss1r</em> revealed spatial changes in the uterus and FT of goats according to the reproductive potential of goats (cyclic versus true anoestrous goats). However, the definitive local role of kisspeptin in the uterus and FT need further investigation.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106850"},"PeriodicalIF":2.1,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140608866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Birth weight, growth indices, and seminal parameters in male offspring are resilient features to maternal pre-conceptional dietary manipulation in sheep","authors":"Cesar A. Rosales-Nieto ,&nbsp;Bradford W. Daigneault ,&nbsp;Jennifer N. Roberts ,&nbsp;Rodrigo Sánchez-López ,&nbsp;Barbara Makela ,&nbsp;Yong Pu ,&nbsp;Richard Ehrhardt ,&nbsp;João H. Jabur Bittar ,&nbsp;Almudena Veiga-Lopez","doi":"10.1016/j.domaniend.2024.106849","DOIUrl":"https://doi.org/10.1016/j.domaniend.2024.106849","url":null,"abstract":"<div><p>Gestational diet manipulation can lead to inadequate fetal nutrient supply resulting in low birth weight, limited postnatal growth, and consequently, reduced reproductive performance in the progeny. However, effects of short-term maternal pre-conceptional dietary manipulation on postnatal growth and reproductive parameters of male offspring in large animals remains unexplored. To determine these consequences, female crossbred (Polypay x Dorset) sheep were allocated to three groups (<em>n</em> = 33/group) of dietary manipulation for 21 days prior to mating under the following conditions: (1) control at 100 % of maintenance energy requirements (40 Kcal of metabolizable energy/kg body weight [BW]), (2) undernutrition (UN) at 50 % of Control intake, and (3) overnutrition (ON) at 200 % of maintenance energy. Singleton ram lambs (UN:9; C:12; ON:6) were monitored from birth until 8 months of age, including birth weight, weekly weights, weight gain, body mass index (BMI), and circulating testosterone. After weaning, monthly scrotal circumference and subcutaneous fat depth were measured. Semen morphology and motility were evaluated at 7 and 8 months of age. Birth weight, weight gain, and BMI at birth and weaning were not significantly different among nutritional treatments. None of the pre-conceptional diets affected body weight change from weaning until 36 weeks of age, BMI, fat depth, or scrotal circumference across the experiment. A sustained rise in plasma testosterone concentrations was detected when ram lambs were, on average, 82 days old and 37 kg. Both testosterone concentrations and scrotal circumference were positively correlated to body weight regardless of treatment group. In addition, seminal parameters did not differ among treatments, but a transient increase in plasma testosterone at 18 weeks of age was observed in ON ram lambs compared to control rams. In conclusion, birth weight, growth indices, and seminal parameters in singleton rams are resilient features in the progeny upon maternal pre-conceptional dietary manipulation in sheep.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106849"},"PeriodicalIF":2.1,"publicationDate":"2024-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0739724024000122/pdfft?md5=13b68f755ef6eb47d3602f4feb1a29af&pid=1-s2.0-S0739724024000122-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140545764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insulin interacts with PPARγ agonists to promote bovine adipocyte differentiation","authors":"Pan−Pan Guo ,&nbsp;Xue−Rui Yao ,&nbsp;Yong−Nan Xu ,&nbsp;Xin Jin ,&nbsp;Qiang Li ,&nbsp;Chang−Guo Yan ,&nbsp;Nam−Hyung Kim ,&nbsp;Xiang−Zi Li","doi":"10.1016/j.domaniend.2024.106848","DOIUrl":"https://doi.org/10.1016/j.domaniend.2024.106848","url":null,"abstract":"<div><p>Insulin is a potent adipogenic hormone that triggers a series of transcription factors that regulate the differentiation of preadipocytes into mature adipocytes. Ciglitazone specifically binds to peroxisome proliferator−activated receptor−<em>γ</em> (<em>PPARγ</em>), thereby promoting adipocyte differentiation. As a natural ligand of <em>PPARγ</em>, oleic acid (OA) can promote the translocation of <em>PPARγ</em> into the nucleus, regulate the expression of downstream genes, and promote adipocyte differentiation. We hypothesized that ciglitazone and oleic acid interact with insulin to enhance bovine preadipocyte differentiation. Preadipocytes were cultured 96 h in differentiation medium containing 10 mg/L insulin (I), 10 mg/L insulin + 10 µM cycloglitazone (IC), 10 mg/L insulin + 100 µM oleic acid (IO), or 10 mg/L insulin + 10 µM cycloglitazone+100 µM oleic acid (ICO). Control preadipocytes (CON) were cultured in differentiation medium (containing 5% fetal calf serum). The effects on the differentiation of Yanbian cattle preadipocytes were examined using molecular and transcriptomic techniques, including differentially expressed genes (DEGs) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis. I, IC, IO, and ICO treatments produced higher concentrations of triglycerides (TAG) and lipid droplet accumulation in preadipocytes compared with CON treatment (<em>P</em> &lt; 0.05). Co−treatment of insulin and <em>PPARγ</em> agonists significantly increased the expression of genes involved in regulating adipogenesis and fatty acid synthesis. (<em>P</em> &lt; 0.05). Differential expression analysis identified 1488, 1764, 1974 and 1368 DEGs in the I, IC, IO and ICO groups, respectively. KEGG pathway analysis revealed DEGs mainly enriched in <em>PPAR</em> signalling, <em>FOXO</em> signaling pathway and fatty acid metabolism. These results indicate that OA, as <em>PPARγ</em> agonist, can more effectively promote the expression of bovine lipogenesis genes and the content of TAG and adiponectin when working together with insulin, and stimulate the differentiation of bovine preadipocytes. These findings provide a basis for further screening of relevant genes and transcription factors in intramuscular fat deposition and meat quality to enhance breeding programs.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106848"},"PeriodicalIF":2.1,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140341348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold exposure affects glucose metabolism, lipid droplet deposition and mitophagy in skeletal muscle of newborn goats","authors":"Duo Su ,&nbsp;Yulong Song ,&nbsp;Die Li ,&nbsp;Shizhong Yang ,&nbsp;Siyuan Zhan ,&nbsp;Tao Zhong ,&nbsp;Jiazhong Guo ,&nbsp;Jiaxue Cao ,&nbsp;Li Li ,&nbsp;Hongping Zhang ,&nbsp;Linjie Wang","doi":"10.1016/j.domaniend.2024.106847","DOIUrl":"https://doi.org/10.1016/j.domaniend.2024.106847","url":null,"abstract":"<div><p>Cold exposure is a common stressor for newborn goats. Skeletal muscle plays an important role in maintaining whole-body homeostasis of glucose and lipid metabolism. However, the molecular mechanisms underlying regulation of skeletal muscle of newborn goats by cold exposure remains unclear. In this study, we found a significant increase (<em>P</em> &lt; 0.01) in serum glucagon levels after 24 h of cold exposure (COLD, 6°C), while glucose and insulin concentrations were significantly decreased (<em>P</em> &lt; 0.01) compared to room temperature (RT, 25°C). Additionally, we found that cold exposure reduced glycogen content (<em>P</em> &lt; 0.01) in skeletal muscle. Pathway enrichment analysis revealed that cold exposure activated skeletal muscle glucose metabolism pathways (including insulin resistance and the insulin signaling pathway) and mitophagy-related pathways. Cold exposure up-regulated the expression of genes involved in fatty acid and triglyceride synthesis, promoting skeletal muscle lipid deposition. Notably, cold exposure induced mitophagy in skeletal muscle.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106847"},"PeriodicalIF":2.1,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140103930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of steroid hormones in the maintenance of focal adhesions in bovine oviductal epithelial cells","authors":"Milda Alejandra Vella ,&nbsp;Daniela Celeste García ,&nbsp;Maximiliano De Boeck ,&nbsp;Pablo Alberto Valdecantos ,&nbsp;Mariela Roldán-Olarte","doi":"10.1016/j.domaniend.2024.106839","DOIUrl":"10.1016/j.domaniend.2024.106839","url":null,"abstract":"<div><p>The oviduct, the organ of the female reproductive system where fertilization and early embryonic development occur, provides an optimal environment for the final maturation of oocytes, storage, and sperm capacitation and transport of gametes and embryos. During the estrous cycle, the oviduct is affected by ovarian sex hormones, resulting in changes aimed at maintaining an appropriate microenvironment. Normal cell migration is tightly regulated, its role being essential for the development and maintenance of organ and tissue functions as well as for regeneration following injury. Due to their involvement in focal contact formations, focal adhesion kinase (PTK2) and paxillin (PXN) are key proteins in the study of cell migration and adhesion. The objective of this work was to compare the expression of <em>PTK2</em> and <em>PXN</em> in oviductal cells along the estrous cycle and to determine if their expression is regulated by the presence of 17-β estradiol (E₂) and/or progesterone (P₄). No transcripts of <em>PTK2</em> or of <em>PXN</em> were detected in cells corresponding to the luteal phase. Additionally, hormonal stimulation experiments on bovine oviductal cell cultures (BOECs) were carried out, where P₄ inhibited the expression of both genes. Migration assays demonstrated that P₄ reduced BOECs migration capacity. P₄ treatment also reduced cell adhesion, while E₂ increased the number of adhered cells. In conclusion, the presence of E₂ and P₄ regulates the expression of genes involved in the formation of focal contacts and modifies the migration and adhesion of BOECs. Understanding the effect of steroid hormones on BOECs is critical to grasp the impact of steroid control on oviductal function and its contribution to establishing successful pregnancies.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"88 ","pages":"Article 106839"},"PeriodicalIF":2.1,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140021146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal and neonatal cortisol and catecholamines throughout vaginal eutocia and C-section in dogs","authors":"Renata Azevedo de Abreu ,&nbsp;Letícia Lima de Almeida ,&nbsp;Maíra Morales de Brito ,&nbsp;Roberto Rodrigues da Rosa Filho ,&nbsp;Maria Cristina Veronesi ,&nbsp;Camila Infantosi Vannucchi","doi":"10.1016/j.domaniend.2024.106838","DOIUrl":"https://doi.org/10.1016/j.domaniend.2024.106838","url":null,"abstract":"<div><p>Increased fetal cortisol is associated with catecholamine release, a primary factor for neonatal adaptive responses. However, relationship between canine peripartum cortisol and catecholamine is unknown. We aimed to compare serum adrenaline, noradrenaline and cortisol during peripartum in bitches and neonates of distinct obstetric conditions and to assess amniotic fluid cortisol concentration. Twenty females and maximum of three puppies per litter were allocated into Vaginal Eutocia (10 females; 17 neonates) and Elective C-section (10 females; 20 neonates) groups. Amniotic fluid was collected at delivery for cortisol concentration. Maternal and neonatal blood were collected prepartum, intrapartum, postpartum and 1h postpartum, and at birth, 30 and 60min, 12hs and 24hs, respectively, for cortisol, adrenaline and noradrenaline assessment. C-section determined higher noradrenaline throughout delivery and cortisol concentration from intrapartum through 1h postpartum, compared to vaginal birth. C-section maternal cortisol showed progressive increase from intrapartum onwards, while neonatal cortisol remained unchanged. No difference of maternal cortisol concentration occurred along whelping, whereas a significant decrease was verified for vaginal delivery puppies from birth until the 12hs. Puppies delivered vaginally had higher cortisol concentration at birth and 30min, compared to c-section puppies. There was a higher concentration of amniotic fluid cortisol in vaginal eutocia. In conclusion, c-section induces higher maternal stress during and after surgery, whilst vaginal delivery is a more neonatal physiologically stressful condition, contributing to better adaptation during transition.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"87 ","pages":"Article 106838"},"PeriodicalIF":2.1,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139942999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Association of vitamin D status with redox balance and insulin resistance and its predicting ability for subclinical pregnancy toxemia in pregnant sheep” [Domestic Animal Endocrinology, Volume: 84 (January 2024) 106823]","authors":"Mehak Nisar ,&nbsp;Shafayat Ahmad Beigh ,&nbsp;Abdul Qayoom Mir ,&nbsp;Syed Ashaq Hussain ,&nbsp;Aijaz Ahmad Dar ,&nbsp;Iqbal Yatoo ,&nbsp;Adil Mehraj Khan","doi":"10.1016/j.domaniend.2023.106835","DOIUrl":"10.1016/j.domaniend.2023.106835","url":null,"abstract":"","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"87 ","pages":"Article 106835"},"PeriodicalIF":2.1,"publicationDate":"2024-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0739724023000516/pdfft?md5=f6ce9a5a374469b5bd80d8ec6b66524f&pid=1-s2.0-S0739724023000516-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139103247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sexual behaviour of young rams is improved and less stressful after intranasal administration of oxytocin","authors":"Juan Pablo Damián ,&nbsp;Laura Castillo ,&nbsp;Victoria Pons ,&nbsp;Jorge Gil ,&nbsp;Gonzalo Suárez ,&nbsp;Angélica Terrazas ,&nbsp;Damian González ,&nbsp;Elize van Lier ,&nbsp;Georgget Banchero","doi":"10.1016/j.domaniend.2023.106837","DOIUrl":"10.1016/j.domaniend.2023.106837","url":null,"abstract":"<div><p>The aim of this study was to determine if intranasal administration of oxytocin modifies sexual behaviour and the stress response in young rams during sexual tests with ewes in oestrus. Ten rams were used in a cross-over design. At Day 0, the control group (CG, n = 5) received isotonic saline spray intranasally, and the treated group (OTG, n = 5) received oxytocin (24 IU) intranasally, 40 min before the sexual test. At Day 15, the groups were reversed. In each sexual test (20 min) with an oestrous-induced ewe, the sexual behaviour of the young rams was recorded. Serum cortisol concentrations were determined before and after the test. Less flehmen was observed in the OTG, but mounts with ejaculation were increased. The OTG presented lower serum cortisol concentration than the CG. In conclusion, intranasal administration of oxytocin modified the sexual behaviour of rams, evidenced by a decrease in flehmen behaviour and an increase in mounts with ejaculation, making sexual activity more efficacious. In addition, the treatment decreased the stress response of the rams in the sexual tests. Therefore, intranasal administration of oxytocin could be used to increase sexual activity in rams, and with less stress, providing better welfare conditions.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"87 ","pages":"Article 106837"},"PeriodicalIF":2.1,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139055671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insulin release from isolated cat islets of Langerhans","authors":"Emma M Strage ,&nbsp;Cecilia Ley ,&nbsp;Gunilla T Westermark ,&nbsp;Anders Tengholm","doi":"10.1016/j.domaniend.2023.106836","DOIUrl":"10.1016/j.domaniend.2023.106836","url":null,"abstract":"<div><p>Feline diabetes mellitus is a common endocrine disease with increasing prevalence. It shows similarities with human type 2 diabetes and is characterized by insulin resistance and deficient insulin secretion. Moreover, cats and humans belong to the very few species that form amyloid depositions in the pancreatic islets. However, little is known about cat islet function and no studies have addressed insulin secretion from isolated islets <em>ex vivo</em>. The aim of this study was to establish a protocol for isolation of islets of Langerhans from pancreata of cats euthanized due to disease, and to evaluate insulin secretion responses to various physiological and pharmacological stimuli. Collagenase digestion of pancreatic tissue from 13 non-diabetic cats and two cats with diabetic ketoacidosis yielded individual islets surrounded by a layer of exocrine tissue that was reduced after two days in culture. Histological examination showed islet amyloid in pancreatic biopsies from most non-diabetic and in one diabetic cat. Islets from non-diabetic cats cultured at 5.5 mM glucose responded with increased insulin secretion to 16.7 mM glucose, 30 mM K⁺ and 20 µM of the sulfonylurea glipizide (2-3 times basal secretion at 3 mM glucose). The glucagon-like peptide-1 receptor agonist exendin-4 (100 nM) had no effect under basal conditions but potentiated glucose-triggered insulin release. Only one of nine islet batches from diabetic cats released detectable amounts of insulin, which was enhanced by exendin-4. Culture of islets from non-diabetic cats at 25 mM glucose impaired secretion both in response to glucose and K⁺ depolarization. In conclusion, we describe a procedure for isolation of islets from cat pancreas biopsies and demonstrate that isolated cat islets secrete insulin in response to glucose and antidiabetic drugs. The study provides a basis for future <em>ex vivo</em> studies of islet function relevant to the understanding of the pathophysiology and treatment of feline diabetes.</p></div>","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"87 ","pages":"Article 106836"},"PeriodicalIF":2.1,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0739724023000528/pdfft?md5=73e74898506fe8a83ccb09639ea1fbeb&pid=1-s2.0-S0739724023000528-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139031864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insulin release from isolated cat islets of Langerhans","authors":"E. Strage, Cecilia Ley, Gunilla T Westermark, Anders Tengholm","doi":"10.1016/j.domaniend.2023.106836","DOIUrl":"https://doi.org/10.1016/j.domaniend.2023.106836","url":null,"abstract":"","PeriodicalId":11356,"journal":{"name":"Domestic animal endocrinology","volume":"990 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139019135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}