IF 1.9 2区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Junyuan Ma, Yanmei Yang, Lin He, Chongfa Yang, Yahua Yang, Yang Li, Wen He, Xiaoying Niu, Zhou Chen, Songming Hu, Jin'e Wang, Yingpai Zhaxi, Shengdong Huo
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引用次数: 0

摘要

雌二醇(E2)由莱狄格细胞(LC)分泌,会因生殖腔收缩而积聚在睾丸中。雌激素不仅对生殖很重要,还能防止炎症。本研究调查了牦牛睾丸间质细胞炎症中的热凋亡作用以及E2对炎症和热凋亡的影响。发炎的睾丸表现出结构性损伤和热解,睾丸液中的E2、睾酮和雌激素受体β(ERβ)水平下降。单用 E2 可抑制睾酮分泌,并增加成熟睾丸细胞中 ERβ 的表达。在 LCs 中,脂多糖(LPS)通过激活 TNF-α 和 IL-6 引起炎症,并通过激活经典和非经典的热解途径引起热解。LPS 可抑制 LCs 中性激素的分泌和 ERβ 的表达。E2可抑制LPS诱导的LCs中ER表达的减少,还可抑制LPS诱导的间质细胞炎症和裂解,Selisistat(EX-527,SIRT1抑制剂)或Fulvestrant(ICI 182,780,E2非基因组受体抑制剂)可部分阻断LPS诱导的间质细胞炎症和裂解。总之,E2通过SIRT1/Nox4/ROS途径缓解了LPS诱导的牦牛LCs炎症和脓毒症。这一发现为了解雌激素在男性生殖健康中的作用提供了新的视角,并为通过调节激素平衡改善睾丸免疫和生殖功能提供了一种潜在的治疗策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
17β-estradiol inhibits lipopolysaccharide-induced inflammation and pyroptosis of Leydig cells of the domestic yak (Bos grunniens) via the SIRT1/Nox4/ROS pathway.

Estradiol (E2) secreted by Leydig cells (LCs) can accumulate in the testes due to constriction of the reproductive lumen. Estrogen is not only important for reproduction, but also protects against inflammation. In this study, the role of pyroptosis in testicular inflammation and the effects of E2 against inflammation and pyroptosis of yak interstitial cells were investigated. Inflamed testes exhibited structural damage and pyroptosis with decreased E2, testosterone, and estrogen receptor β (ERβ) levels in testicular fluid. E2 alone inhibited testosterone secretion and increased ERβ expression in mature LCs. In LCs, lipopolysaccharide (LPS) causes inflammation by activation of TNF-α and IL-6, and pyroptosis via activation of the classical and non-classical pyroptosis pathways. LPS inhibits sex hormone secretion and ERβ expression in LCs. E2 inhibited the LPS-induced decrease of ER expression in LCs and also inhibited LPS-induced interstitial cell inflammation and pyroptosis, which was partially blocked by Selisistat (EX-527, SIRT1 inhibitor) or Fulvestrant (ICI 182,780, E2 non-genomic receptor inhibitors). In conclusion, E2 relieved LPS-induced inflammation and pyroptosis of yak LCs via the SIRT1/Nox4/ROS pathway. This finding provides new insights into the role of estrogen in male reproductive health and offers a potential therapeutic strategy to improve testicular immune and reproductive function by modulating hormonal homeostasis.

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来源期刊
Domestic animal endocrinology
Domestic animal endocrinology 农林科学-奶制品与动物科学
CiteScore
5.50
自引率
4.80%
发文量
58
审稿时长
31 days
期刊介绍: Domestic Animal Endocrinology publishes scientific papers dealing with the study of the endocrine physiology of domestic animal species. Those manuscripts utilizing other species as models for clinical or production problems associated with domestic animals are also welcome. Topics covered include: Classical and reproductive endocrinology- Clinical and applied endocrinology- Regulation of hormone secretion- Hormone action- Molecular biology- Cytokines- Growth factors
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