Dentistry Journal最新文献

{"title":"Impact of Molar Distalization with Clear Aligners on Periodontal Ligament Stress and Root Resorption Risk: A Systematic Review of 3D Finite Element Analysis Studies.","authors":"Ava Nazeri, Jose A Castillo, Arash Ghaffari-Rafi","doi":"10.3390/dj13020065","DOIUrl":"10.3390/dj13020065","url":null,"abstract":"<p><p><b>Background/Objectives:</b> Molar distalization with clear aligners is increasingly used for Class II malocclusions, yet the associated periodontal ligament (PDL) stress and potential root resorption risk remain unclear. Three-dimensional finite element analysis (3D FEA) provides insight into these factors, but variations in attachments and anchorage strategies merit systematic evaluation. To determine whether molar distalization with clear aligners exceeds the PDL stress thresholds for root resorption and to assess how different attachments and anchorage methods influence stress distribution. <b>Methods:</b> In accordance with the PRISMA 2020 guidelines, four electronic databases were searched without language or date restrictions. Studies were included if they (1) employed 3D FEA, (2) analyzed PDL stress during aligner-based molar distalization, and (3) assessed root resorption risk or stress thresholds. Two reviewers independently screened and extracted data, yielding eight studies. <b>Results:</b> Attachments lowered PDL stress and distributed forces more evenly, reducing root resorption risk compared with no attachment cases. Micro-implants shifted stress to molars and protected anterior teeth; palatal mini-screws achieved greater distalization but higher stress, requiring caution, while buccal mini-screws showed lower stress in first premolar roots. Placing a mini-screw between first and second molars yielded the lowest, most uniform stress. Class II elastics-with precision cuts-demonstrated low compressive stress and improved anchorage, although some resorption risk persisted in maxillary anteriors. <b>Conclusions:</b> Clear aligner-based molar distalization can elevate PDL stress to potentially resorptive levels. Although attachments, micro-implants, and Class II elastics improve stress distribution and lessen root resorption risk, it is not fully eliminated. Careful, individualized treatment planning remains essential, and further clinical validation is needed to establish definitive guidelines.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"13 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854506/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanical Behaviour of Orthodontic Auxiliary Photopolymerisable Resins in Simulated Oral Conditions: An In Vitro Study.","authors":"Riccardo Favero, Tommaso Zanetti, Vincenzo Tosco, Riccardo Monterubbianesi, Andrea Volpato","doi":"10.3390/dj13020067","DOIUrl":"10.3390/dj13020067","url":null,"abstract":"<p><strong>Background: </strong>The widespread adoption of clear aligners in orthodontic practice has driven the development of biomechanical devices to improve treatment efficiency. The mechanical properties of these materials play a critical role in determining their clinical performance and efficacy. This study investigates the Young's modulus of Clear-Blokker^® (Scheu Dental), a photopolymerisable resin used for the attachment of clear aligner, and evaluates its mechanical behaviour under different curing times (5 s and 10 s) and environmental conditions (dry storage and immersion in artificial saliva at 37 °C).</p><p><strong>Methods: </strong>Forty-eight cylindrical specimens were prepared and subjected to quasistatic compression tests after 14 days. A multi-factorial analysis of variance (ANOVA) at a significance level of 5% was performed to compare the variances.</p><p><strong>Results: </strong>The results showed that samples immersed in artificial saliva had significantly reduced Young's moduli compared to samples stored in dry conditions (<i>p</i> = 0.0213), while no significant difference was observed between curing times.</p><p><strong>Conclusions: </strong>The results suggest that Clear-Blokker^® has mechanical properties comparable to those of clear aligner materials, making it suitable as a biomechanical aid for orthodontic treatment. However, further clinical studies are required to confirm its long-term efficacy in the oral environment.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"13 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854066/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatments for Recurrent Aphthous Stomatitis: A Literature Review.","authors":"Maurizio D'Amario, Giordano Foffo, Filippo Grilli, Mario Capogreco, Tommaso Pizzolante, Sofia Rastelli","doi":"10.3390/dj13020066","DOIUrl":"10.3390/dj13020066","url":null,"abstract":"<p><strong>Background/objectives: </strong>This literature review aims to provide guidance on the treatment of recurrent aphthous stomatitis (RAS) based on studies published over the past 10 years.</p><p><strong>Methods: </strong>This study included randomized clinical trials involving human patients from 2013 and 2023, published in any language. The trials examined both pharmacological and non-pharmacological treatments for aphthous lesions, mainly focusing on the adult population, with pain management as the primary outcome. The research was conducted using PubMed, EMBASE, and CINHAL databases.</p><p><strong>Results: </strong>Most of the 45 analyzed studies focused on non-pharmacological therapies, which led to positive results with minimal adverse effects or contraindications, even when compared to cortisone-based treatments. Laser therapy also showed excellent results, particularly in the immediate post-treatment period. Non-pharmacological therapies appeared to offer the best risk-benefit ratio for patients suffering from RAS.</p><p><strong>Conclusions: </strong>Treatment should be individualized based on the patient's specific form of RAS, and laser therapy can be used either as a standalone treatment or as an adjunct to other treatments considered in the review.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"13 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11853750/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is the Modified Dental Anxiety Scale (MDAS) a Single or Two Construct Measure? A Theoretical and Pragmatic Perspective.","authors":"Gerald Michael Humphris, Jonathan Timothy Newton","doi":"10.3390/dj13020068","DOIUrl":"10.3390/dj13020068","url":null,"abstract":"<p><p><b>Background:</b> The MDAS questionnaire is one of a number of scales available to assess dental anxiety. It is widely used and translated into many world languages; however, it lacks an explicit theoretical backdrop to the content and structure of the measure. This paper draws upon original expositions of dental anxiety: how it develops, is maintained, and how this draws attention to a re-evaluation of the measure. To assist this inspection it was proposed to investigate a two latent construct formulation through a stepwise analysis using data from a representative survey of English respondents on their oral health (the Adult Dental Health Survey). <b>Aim:</b> To present a brief theoretical framework to underpin the measure and, as part of this study's objectives, to provide some evidence to support the measure's potential two-construct structure. <b>Method:</b> Narrative review, structural equation modelling, and testing of specific associations to indicate a two latent construct formulation. Data included the MDAS items (where items 1 and 2 comprise the anticipatory subscale, and items 3 to 5 describe the treatment-related subscale). These items were completed by the representative sample of respondents from the most recent Adult Dental Health Survey conducted in 2009. <b>Results:</b> The two latent construct solution for describing dental anxiety was supported. The anticipated and treatment-related subscales could be discriminated, although they were strongly correlated, demonstrating overlap. Comparison of how each construct varied across the three age groups suggests an interesting heterogeneity. In addition, the two constructs behaved differently when acknowledging previous experience of respondents' last dental visit. Anticipatory dental anxiety was more strongly related to oral health-related quality of life (OHRQoL) than treatment-related dental anxiety as predicted. This partial evidence from empirical data and previous reports in other studies suggests that the separation of the MDAS measure into the two subscales may be warranted. <b>Discussion:</b> Researchers are recommended to report not only the total score of the MDAS in their studies but also consider presenting the two subscale scores, namely, anticipatory and treatment-related dental anxiety. Further work is indicated to determine if clinicians may find the subscales of use when assessing their patients.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"13 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An In-Vitro Investigation into the Fracture Resistance of Prefabricated and Custom-Made Zirconia Crowns for Permanent Molars in Children.","authors":"Thikrayat Bani-Hani, Rami S Al-Fodeh, Ahed M Al-Wahadni, Elham S Abu-Alhaija, Mahmoud Al-Hakam","doi":"10.3390/dj13020064","DOIUrl":"10.3390/dj13020064","url":null,"abstract":"<p><p><b>Background:</b> Recently, the demand for esthetic restorations has grown dramatically and extended into the pediatric population. The prefabricated zirconia crowns (PZCs) and custom-made zirconia crowns (CZCs) are new esthetic options in pediatric dentistry. However, they are still inadequately tested for use in children. Aim: To determine the fracture resistance and failure mode of the PZC in comparison to the CZC. <b>Materials and Methods:</b> In this in-vitro study, thirty cobalt-chromium dies were fabricated by scanning the negative replica of a prefabricated lower first permanent molar zirconia crown. CZCs were designed and milled using two different zirconia brands: Ceramill Zolid-FX (FX) and the Highly-Translucent (HT) zirconia. Dies were randomly assigned to receive either a PZC or a CZC (n = 10 in each group). All crowns were cemented on their respective dies using glass ionomer cement. Following artificial aging, all specimens were loaded to failure. Fracture mode analysis was performed. One-way ANOVA and Bonferroni post-hoc test were used for multiple comparisons across the groups. The significant level was set at <i>p</i> ≤ 0.05. <b>Results:</b> HT zirconia had a significantly higher fracture load compared to other groups (<i>p</i> < 0.05). The mean fracture resistance values were: (3087 ± 385) N for HT zirconia, (2633 ± 300) N for PZCs, and (2483 ± 381) N for FX, with no statistically significant difference in fracture strengths between PZCs and FX. <b>Conclusions:</b> HT zirconia crowns showed the highest fracture resistance amongst all groups. The fracture loads of tested crowns exceeded the maximum posterior biteforce. When placed in permanent molars, PZC are expected to perform well under masticatory forces in children.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"13 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Survey of Physicians' Knowledge and Practices Towards Oral Appliance Therapy for Obstructive Sleep Apnea Treatment.","authors":"Chaniporn Ananwattananon, Supatchai Boonpratham, Yodhathai Satravaha, Chaiyapol Chaweewannakorn, Supakit Peanchitlertkajorn","doi":"10.3390/dj13020063","DOIUrl":"10.3390/dj13020063","url":null,"abstract":"<p><strong>Background: </strong>Oral appliance therapy (OAT) is an effective treatment alternative for obstructive sleep apnea (OSA). Understanding physicians' knowledge and practices regarding OAT is crucial for improving OSA treatment, particularly in Southeast Asia, where there is limited research on this topic.</p><p><strong>Objectives: </strong>This study sought to survey physicians' knowledge and practices regarding OAT for OSA treatment.</p><p><strong>Methods: </strong>A descriptive cross-sectional survey was conducted among Thai physicians attending the annual Sleep Society of Thailand meeting. The questionnaire included questions on demographics (six items), educational background (four items), OAT knowledge (two items), indications (three items), effectiveness (seven items), referral practices (five items), treatment planning (eight items), and the importance of OAT education (one item). Descriptive and comparative analyses were performed using SPSS to assess differences in knowledge and referral practices across various demographics.</p><p><strong>Results: </strong>This study achieved a response rate of 51.7% (30/58), with the respondents averaging 37.8 ± 6.0 years in age and 13.7 ± 6.7 years in practice. Although over 66.7% of the respondents recognized OAT's effectiveness and understood its indications, only 36.7% regularly prescribed OAT, and 57.7% referred patients to specific dentists. Knowledge and referral practices differed significantly based on years of practice and postgraduate training in sleep medicine. Physicians with 11-20 years of practice demonstrated higher pediatric OAT knowledge scores compared to those with less experience. Those with postgraduate training achieved higher adult OAT knowledge scores and had greater referral experience.</p><p><strong>Conclusions: </strong>Despite the general awareness and good knowledge of OAT among physicians, clinical use and referral rates remain low. To bridge the gap between knowledge and practice, it is recommended that postgraduate training in dental sleep medicine is promoted and further research is conducted to identify barriers to the adoption of OAT.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"13 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Home Efficacy of a Postbiotic-Based Gel Compared with a Gel Without Active Ingredients for the Treatment of Gingival Inflammation in Patients with Down Syndrome: A Randomized Controlled Study.","authors":"Andrea Scribante, Paolo Appendino, Carolina Maiorani, Paolo Fontanarosa, Maurizio Pascadopoli, Sara Cammisuli, Basmala Azouz, Simone Buttiglieri, Andrea Butera","doi":"10.3390/dj13020062","DOIUrl":"10.3390/dj13020062","url":null,"abstract":"<p><strong>Objectives: </strong>The aim of this six-month randomized controlled study was to evaluate the efficacy of a non-surgical periodontal treatment combined with the use of an active gel compared to a non-surgical treatment alone in reducing inflammatory indices in periodontal patients with Down syndrome.</p><p><strong>Methods: </strong>A total of 40 patients were included in the study, 20 of which were assigned to the active group and 20 to the control group. The active group underwent non-surgical periodontal treatment supplemented by daily home application of an intensive soothing gel containing probiotics. The control group received non-surgical periodontal treatment combined with the application of a gel without active ingredients. The following clinical indices were assessed: Bleeding on Probing (BOP %), Plaque Control Record (PCR %), Mobility (Miller Index), and Modified Marginal Gingival Index (MGI). Measurements were taken at baseline (T0), one month after treatment initiation (T1), after three months (T2), and after six months (T3). The patient compliance was evaluated at each visit, and product satisfaction was assessed through a questionnaire using a Visual Analogue Scale (VAS).</p><p><strong>Results: </strong>By the end of the study, statistically significant improvements were observed in both the groups from T0 to T3 evaluation (<i>p</i> < 0.05). The BOP score was significantly lower in the Trial group at the T3 intergroup evaluation (<i>p</i> < 0.05).</p><p><strong>Conclusions: </strong>A soothing gel with postbiotic and natural compounds was a valuable adjunct to non-surgical periodontal treatment to improve periodontal health in patients with Down syndrome, reducing BOP after 6 months of treatment.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"13 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathophysiological Responses of Oral Keratinocytes After Exposure to Flavored E-Cigarette Liquids.","authors":"Abrar Shamim, Hannah Herzog, Raivat Shah, Sara Pecorelli, Virginia Nisbet, Ann George, Giancarlo A Cuadra, Dominic L Palazzolo","doi":"10.3390/dj13020060","DOIUrl":"10.3390/dj13020060","url":null,"abstract":"<p><strong>Background: </strong>Electronic cigarettes (ECIGs) have grown in popularity, particularly among adolescents and young adults. Flavored ECIG-liquids (E-liquids) are aerosolized by these ECIGs and inhaled into the respiratory system. Several studies have shown detrimental effects of E-liquids in airway tissues, revealing that flavoring agents may be the most irritating component. However, research on the effects of E-liquids on biological processes of the oral cavity, which is the first site of aerosol contact, is limited. Hence, this study focuses on the effects of E-liquid flavors on oral epithelial cells using the OKF6/TERT-2 cell line model.</p><p><strong>Methodology: </strong>E-liquid was prepared with and without flavors (tobacco, menthol, cinnamon, and strawberry). OKF6/TERT-2 oral epithelial cells, cultured at 37 °C and 5% CO₂, were exposed to 1% E-liquid ± flavors for 24 h. Outcomes determined include cell morphology, media pH, wound healing capability, oxidative stress, expression of mucin and tight junction genes, glycoprotein release, and levels of inflammatory cytokines (TNFα, IL-6, and IL-8).</p><p><strong>Results: </strong>Exposure to 1% flavored E-liquids negatively affect cellular confluency, adherence, and morphology. E-liquids ± flavors, particularly cinnamon, increase oxidative stress and production of IL-8, curtail wound healing recovery, and decrease glycoprotein release. Gene expression of <i>muc5b</i> is downregulated after exposure to E-liquids. In contrast, E-liquids upregulate <i>occludin</i> and <i>claudin-1</i>.</p><p><strong>Conclusions: </strong>This study suggests that ECIG use is not without risk. Flavored E-liquids, particularly cinnamon, result in pathophysiological responses of OKF6/TERT-2 cells. The dysregulation of inflammatory responses and cellular biology induced by E-liquids may contribute to various oral pathologies.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"13 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854566/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orthodontic Management of Different Stages and Grades of Periodontitis According to the 2017 Classification of Periodontal Diseases.","authors":"Nada Tawfig Hashim, Shahistha Parveen Dasnadi, Hassan Ziada, Muhammed Mustahsen Rahman, Ayman Ahmed, Riham Mohammed, Md Sofiqul Islam, Rohan Mascarenhas, Bakri Gobara Gismalla, Neamat Hassan Abubakr","doi":"10.3390/dj13020059","DOIUrl":"10.3390/dj13020059","url":null,"abstract":"<p><p><b>Background/Objectives:</b> The 2017 Periodontal Classification offers a comprehensive framework for the diagnosis and management of periodontitis based on staging and grading criteria. Orthodontic therapy is increasingly incorporated into the management of periodontitis to rectify malocclusion, pathological tooth migration, and occlusal stability. Nonetheless, few data directly correspond with this revised classification scheme. The objective of this systematic review is to figure out the influence of orthodontic therapy on periodontal outcomes in patients with Stage III and IV periodontitis, as categorized by the 2017 framework. <b>Methods</b>: A systematic review was performed in accordance with the PRISMA 2020 principles. The databases examined were PubMed, Web of Science, Scopus, and Google Scholar. The evaluation focuses on research published from 2012 to 2024. Seventeen studies were assessed after the application of the inclusion criteria. Key outcomes included clinical attachment level (CAL) improvement, probing depth (PD) decrease, and radiographic bone fill. <b>Results</b>: The integration of orthodontic treatment with periodontal therapy markedly enhanced CAL (mean gain: 4.35-5.96 mm), decreased PD (mean reduction: 3.1-6.3 mm), and facilitated radiographic bone regeneration (mean vertical fill: 4.89 mm). Patients with Stage IV Grade C periodontitis had the most significant improvement, especially with early orthodontic intervention subsequent to regenerative treatment. Prolonged follow-ups (up to 10 years) validated consistent results. <b>Conclusions</b>: Orthodontic intervention, as a supplementary measure to periodontal therapy, improves results in severe periodontitis, especially in Stage III and IV patients. These results underscore the need for multidisciplinary teamwork and defined protocols for including orthodontics in periodontitis therapy.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"13 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11853809/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of Podoplanin-Deficient Mouse Bone with Mechanical Stress.","authors":"Takenori Kanai, Kyoko Osawa, Koichiro Kajiwara, Yoshiaki Sato, Yoshihiko Sawa","doi":"10.3390/dj13020061","DOIUrl":"10.3390/dj13020061","url":null,"abstract":"<p><p><b>Objective:</b> We investigated morphological differences in osteocyte processes between aged mice and our original podoplanin-conditional knockout (cKO) mice in which the floxed exon 3 of podoplanin was deleted by Dmp-1-driven Cre (Dmp1-Cre;PdpnΔ/Δ). <b>Methods:</b> SEM observation on osteocyte cell process, histochemistry for bone remodeling with mechanostress, and RT-PCR for RANKL and M-CSF in podoplanin cKO mouse bone with mechanostress was investigated. <b>Results:</b> SEM observations showed fewer and thinner osteocyte processes in femurs from 23-week-old Dmp1-Cre;PdpnΔ/Δ mice than from 23-week-old wild-type mice, while the numbers of osteocyte processes in femurs and calvarias were similar in 23-week-old Dmp1-Cre;PdpnΔ/Δ mice and 48-week-old wild-type mice. Furthermore, cell process numbers in femurs and calvarias were significantly smaller in 23-week-old Dmp1-Cre;PdpnΔ/Δ mice than in 48-week-old wild-type mice. In the test for differences in alveolar bone resorption under mechanical stress between Dmp1-Cre;PdpnΔ/Δ and wild-type mice, the area of TRAP-positive resorption pits was larger in wild-type mice than in Dmp1-Cre;PdpnΔ/Δ mice. In a quantitative tissue PCR analysis, the mRNA expression levels of RANKL and M-CSF in alveolar bone under mechanical stress were significantly lower in Dmp1-Cre;PdpnΔ/Δ mice than in wild-type mice. These results suggest that a reduction in cell process formation in osteocytes with podoplanin cKO affected the absorption of alveolar bone under mechanical stress in Dmp1-Cre;PdpnΔ/Δ mice. <b>Conclusions:</b> In podoplanin-deficient bone, the deformation of osteocyte processes by mechanical stimuli is not recognized as a stress due to the lower number of cell processes with podoplanin deficiency; therefore, the production of osteoclast migration/differentiation factors by activated osteocytes is not fully induced and macrophage migration to alveolar bone with mechanical stress appeared to be suppressed. These results indicate that podoplanin-dependent osteocyte process formation indirectly plays a key role in sensing mechanical stress in bone.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"13 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11854086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}