Developmental and comparative immunology最新文献

{"title":"Lamprey TFE3 exhibits evolutionarily conserved activation mechanisms and regulates autophagy and immune responses","authors":"Jilong Pan ,&nbsp;Xinping Li ,&nbsp;Qipeng Zhang ,&nbsp;Jiarui Li ,&nbsp;Daiyun Zhang ,&nbsp;Xiaoyu Ni ,&nbsp;Pengcheng Li ,&nbsp;Tiesong Li ,&nbsp;Hao Wang ,&nbsp;Yan Chi","doi":"10.1016/j.dci.2025.105480","DOIUrl":"10.1016/j.dci.2025.105480","url":null,"abstract":"<div><div>The microphthalmia-associated transcription factor/transcription factor E (MiT/TFE) family belongs to the basic group of helix-loop-helix leucine zipper (bHLH-ZIP)-containing transcription factors. Although MiT/TFE family members, especially TFEB and TFE3, have been extensively studied in higher vertebrates, little is known about their roles in basal vertebrates such as lampreys. Here, we show that lamprey TFE3 and TFEC retain conserved structural features, including key domains, motifs, and serine residues, consistent with the evolutionary history of vertebrates. Expression profiling revealed that lamprey TFE3 is broadly expressed across tissues, with the highest level in the heart. Treatment with Torin1 induced nuclear translocation of TFE3, indicative of its activation. Torin1 also led to a time-dependent increase in TFE3 expression, and upregulation of downstream target genes associated with autophagy, stress responses, and inflammation. Furthermore, we observed elevated LC3-II levels and reduced p62 expression following Torin1 treatment, indicating that activated lamprey TFE3 enhances autophagic activity. Notably, TFE3 activation also promoted cholesterol mobilization and efflux, as evidenced by decreased Bodipy fluorescence intensity and upregulated expression of ABCA1. In vivo stimulation with LPS or poly (I:C) induced significant changes in Lj-TFE3 expression, indicating that lamprey TFE3 is responsive to pathogen-associated molecular patterns and may participate in immune stress responses. Together, these findings demonstrate that lamprey TFE3 exhibits conserved structural and functional features and plays a key role in immune and metabolic regulation, providing important evolutionary insights into the MiT/TFE transcription factor family in early vertebrates.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105480"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Persistence of viral load in shrimp that survived WSSV infection","authors":"Phasini Buathongkam ,&nbsp;Jiraporn Srisala ,&nbsp;Chanisara Srivihok ,&nbsp;Sithichoke Tangphatsornruang ,&nbsp;Christopher J. Coates ,&nbsp;Suparat Taengchaiyaphum ,&nbsp;Siripong Thitamadee ,&nbsp;Kallaya Sritunyalucksana","doi":"10.1016/j.dci.2025.105488","DOIUrl":"10.1016/j.dci.2025.105488","url":null,"abstract":"<div><div>We established a cohabitation model to study shrimp survival after a white spot syndrome virus (WSSV) outbreak. Naïve shrimp were reared individually in plastic boxes immersed in a tank with ten free-roaming shrimp injected each with 1000 copies of purified WSSV. A WSSV outbreak commenced from day four (elevated mortality levels), which lasted for about 10 days. When no further mortalities occurred, surviving shrimp were collected for observation. Survival levels of the cohabitating shrimp were between 5.3 % and 15.9 % from independent infection trials. Determination of viral loads by qPCR and RT-PCR demonstrated 10,000-fold higher viral copy numbers in the moribund shrimp than in the survivors. Western blot analysis using an <em>anti</em>-VP28 antibody confirmed PCR results that high VP28 expression occurs in moribund shrimp, but no signals were detected in the survivors. Histological examination depicted eosinophilic inclusion bodies with hypertrophied (swollen) nuclei and marginated, slightly basophilic, chromatin in the moribund shrimp, but not in the survivors. These data suggest that the surviving shrimp are resilient and posses a mechanism to curtail viremia. Expression levels of selected antimicrobial factors – <em>ALF3</em>, <em>ALF6</em>, <em>PmCrustin1</em>, <em>PmPenaeidin3</em> and <em>PmPenaeidin5</em> were compared between moribund and survivor shrimp. <em>PmCrustin1</em> and <em>ALF3</em> expression were substantially higher in the moribund shrimp than those of survivors. Interestingly, expression levels of <em>PmCrustin1</em> were correlated positively with viral loads<em>.</em> Our data provides new insight into WSSV resilience in <em>Penaeus monondon.</em></div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105488"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145279235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic characterization and host immune responses to Decapod iridescent virus 1 (DIV1) in Macrobrachium rosenbergii","authors":"Jingwen Hao ,&nbsp;Yukun Jie ,&nbsp;Zhibin Lu ,&nbsp;Tiantian Ye ,&nbsp;Jilun Meng ,&nbsp;Cui Liu ,&nbsp;Junjun Yan ,&nbsp;Yutong Zheng ,&nbsp;Zaijie Dong ,&nbsp;Zhimin Gu","doi":"10.1016/j.dci.2025.105491","DOIUrl":"10.1016/j.dci.2025.105491","url":null,"abstract":"<div><div>Decapod iridescent virus 1 (DIV1) is an emerging virus associated with high morbidity and mortality in crustaceans. In this study, we obtained a novel, highly pathogenic strain of DIV1, named DIV1-ZH, and performed whole-genome sequencing. The full length of the DIV1-ZH genome is 166,964 bp, with a GC content of 34.56 %. A total of 176 open reading frames (ORFs) were identified. Based on the phylogenetic analysis of the predicted major capsid protein (MCP), DIV1-ZH has a high homology with CQIV and SHIV. The 50 % lethal dose (LD₅₀) of the DIV1-ZH strain for <em>M. rosenbergii</em> at 72 h post-infection was estimated to be 1.30 × 10⁸ copies/mL, accompanied by characteristic clinical signs such as a white triangle at the rostrum base and gut emptiness. Viral load measurements showed rapid viral replication in hepatopancreas, gills, and intestine from 0 to 72 hpi. Histopathology revealed eosinophilic inclusions, nuclear pyknosis, parenchymal necrosis, and immune cell infiltration in the hepatopancreas. Electron microscopy confirmed the presence of icosahedral viral particles in the cytoplasm of hepatopancreatic and intestinal cells. In addition, qRT-PCR revealed significant upregulation of immune-related genes (<em>TPI</em>, <em>RSAD2</em>, <em>Caspase3</em>, <em>Crustin</em>, <em>ALF2</em>, <em>ALF5</em>) in infected tissues, indicating a robust host immune response. These results provide new insights into the molecular pathogenesis and host interactions of DIV1-ZH, and lay the groundwork for future prevention and control strategies in crustacean aquaculture.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105491"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunometabolic characteristics in liver of red crucian carp (Carassius auratus red var.) following Edwardsiella tarda 1l-4 infection by multiomics analyses","authors":"Ting Luo ,&nbsp;Ruo-Xing Yu ,&nbsp;Qin-Yang He ,&nbsp;Zi-Rou Zhong ,&nbsp;Zhuang-Wen Mao ,&nbsp;Ming-Zhu Huang ,&nbsp;Jie Peng ,&nbsp;Yao-Hui Li ,&nbsp;Zi-Le Qin ,&nbsp;Xu-Ying Kuang ,&nbsp;Zi-Xuan Fang ,&nbsp;Jian Li ,&nbsp;Sheng-Wei Luo","doi":"10.1016/j.dci.2025.105479","DOIUrl":"10.1016/j.dci.2025.105479","url":null,"abstract":"<div><div><em>Edwardsiella tarda,</em> a facultative intracellular pathogen, can compromise the health of farmed fish. Nevertheless, the immunometabolic features of red crucian carp (<em>Carassius auratus</em> red var.) after <em>E. tarda</em> 1l-4 infection remains largely understudied. In this investigation, severe tissue injury and aberrant glycogen storage were detected in liver of red crucian carps infected with <em>E. tarda</em> 1l-4, along with the dramatic decline of antioxidant defense. Multiomics approaches indicated that MAPK signaling pathway may assume a dominant role in immunometabolic regulation in red crucian carp. MAPK dysregulation may affect cell cycle and DNA replication, while also disrupting carbon metabolism, amino acid homeostasis and glycerophospholipid biosynthesis, with methylsuccinic acid (MSA) identified as pivotal metabolic indicator. This findings may deepen our understanding of <em>E. tarda</em>-induced immunometabolic responses in red crucian carps and provide practical references for edwardsiellosis management in aquaculture.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105479"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SIGIRR deficiency aggravates Mycobacterium marinum induced mortality and hepatic apoptosis in zebrafish","authors":"Ting Yu ,&nbsp;Haoxiang Chen ,&nbsp;Peipei Yu ,&nbsp;Simei Hu ,&nbsp;Kai Luo ,&nbsp;Weihua Gao ,&nbsp;Hanwen Yuan ,&nbsp;Zhang Jingchen ,&nbsp;Yang Xuefen ,&nbsp;Shuhuan Zhang ,&nbsp;Qiaoqing Xu ,&nbsp;Qingping Lian","doi":"10.1016/j.dci.2025.105499","DOIUrl":"10.1016/j.dci.2025.105499","url":null,"abstract":"<div><div>SIGIRR is a cell membrane protein in the TIR superfamily, widely expressed in tissues and organs. It has a unique structure and acts as a negative regulator of downstream inflammatory signaling pathways. <em>Danio rerio</em> (zebrafish) were experimentally infected with <em>Mycobacterium marinum</em> to investigate the role of SIGIRR in modulating host immune responses to bacterial infection. Following intraperitoneal injection of <em>M. marinum</em>, SIGIRR gene-deficient zebrafish exhibited an earlier onset of mortality compared to wild type, with the first death occurring sooner and all individuals dying by the fifth week. Wild-type zebrafish began dying in week two and all died by week seven, while SIGIRR^−/− mutants died significantly faster. A zebrafish liver cell model was established, and apoptosis was measured by flow cytometry 24 h post-infection. Apoptosis was 16 % in wild-type cells and 25 % in SIGIRR^−/− mutant cells. The addition of SIGIRR polyclonal antibody to wild-type liver cells increased apoptosis to 18 % after M. marinum challenge. Significant differences were observed among the three groups. These results show that SIGIRR critically suppresses the inflammatory response during bacterial infection.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105499"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145344056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization and antioxidant function of glutaredoxin 3 in Sepiella japonica","authors":"Yan Lu ,&nbsp;Peipei Fu ,&nbsp;Jiaxin Gao ,&nbsp;Yingying Ye ,&nbsp;Xinqin Jiang ,&nbsp;Denghui Zhu","doi":"10.1016/j.dci.2025.105487","DOIUrl":"10.1016/j.dci.2025.105487","url":null,"abstract":"<div><div>Glutaredoxins (Grxs) are essential antioxidant proteins that protect cells from oxidative damage and participate in multiple biological processes. However, their roles in the common Chinese cuttlefish (<em>Sepiella japonica</em>) remain uncharacterized. Here, we identified a glutaredoxin 3 homolog from <em>S. japonica</em> (<em>SjGrx3</em>). The <em>SjGrx3</em> gene encodes a 981 bp open reading frame producing a 326-amino-acid protein (36.47 kDa) that contains an N-terminal thioredoxin domain and two C-terminal Grx domains, each containing the conserved CGFS active-site motif. Phylogenetic analysis clustered SjGrx3 within the cephalopod Grx3 clade, showing high sequence identity to other molluscan homologs. The <em>SjGrx3</em> mRNA was detected in all examined tissues, with the highest levels in skin and kidney and moderate levels in gill, gut, and liver. In naturally <em>Vibrio harveyi</em>-infected individuals, <em>SjGrx3</em> transcripts increased significantly in skin, gill, liver, white body, and brain, implicating it in innate immune responses. Confocal microscopy showed that SjGrx3 localized to both the cytoplasm and nucleus. Functional assays demonstrated that recombinant SjGrx3 was soluble and enhanced <em>Escherichia coli</em> resistance to H₂O₂-induced oxidative damage. Moreover, overexpression of SjGrx3 in HEK293T cells significantly reduced H₂O₂-induced intracellular reactive oxygen species (ROS) accumulation and inhibited H₂O₂-mediated cell death, confirming its role in oxidative stress protection. Collectively, these findings indicate that <em>SjGrx3</em> contributes to redox regulation and immune defense against bacterial infection in <em>S. japonica</em>.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105487"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145274117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The migration of cells from the water-vascular system to the coelom provides evidence supporting the origin of coelomocytes in sea cucumber Apostichopus japonicus","authors":"Zhenhui Wang ,&nbsp;Yupeng Chen ,&nbsp;Yinan Wang ,&nbsp;Xuyuan Fan ,&nbsp;Yuan Ren ,&nbsp;Chang Wei ,&nbsp;Qiang Li","doi":"10.1016/j.dci.2025.105509","DOIUrl":"10.1016/j.dci.2025.105509","url":null,"abstract":"<div><div><em>Apostichopus japonicus</em>, a member of Holothuroidea (phylum Echinodermata), is known for its strong ability to regenerate its coelomocytes. The constitutive recovery of coelomocytes occurs at 6 h post-evisceration. Rapid coelomocyte recovery, combined with limited proliferation of residual coelomocytes at the early recovery stage, suggests that migration from other sites is the likely mechanism. In this study, we investigated migration of coelomocytes and several other components, including cell-free coelomic fluid, protein, and bacteria from the water-vascular system to the coelom by transplantation of these components through the papillae. The coelomic fluid had migrated substantially at 6 h post-transplantation (hpt). Glutathione S-transferase was detected migrating into the coelom at 6 and 12 hpt with an increasing trend. <em>Vibrio splendidus</em>, a pathogenic bacterium, and <em>Escherichia coli</em>, a non-pathogenic bacterium of <em>A. japonicus</em>, were both found to migrate at 6–72 hpt. Fluorescence labeling and allogeneic transplantation of live coelomocytes were used to investigate coelomocyte migration. Coelomocytes migrated from the water-vascular system to the coelom at 2 and 12 hpt, with a migration rate of 1.2 % and 3.7 %, respectively. However, migration rate increased significantly at 6 and 12 hpt under evisceration, which were 2.5- and 1.3-fold than that in healthy <em>A. japonicus</em>. Pathogenic infection significantly increased coelomocyte migration rate at each point post-transplantation, with a peak of 7.60-fold at 48 hpt. The findings have shown that different components can migrate from the water-vascular system to the coelom in <em>A. japonicus</em>. This suggests that the water-vascular system may act as a storage pool for coelomocytes to supplement coelomocyte physiological loss. This study enhances our understanding of the immune defense mechanisms and regenerative processes in echinoderms.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105509"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145457730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathological insights and molecular responses to Morganella morganii infection in largemouth bass (Micropterus salmoides)","authors":"Wenji Huang ,&nbsp;Xiaoming Zhang ,&nbsp;Sijia Wan,&nbsp;Yanan Liu,&nbsp;Lan He,&nbsp;Ling Shao","doi":"10.1016/j.dci.2025.105497","DOIUrl":"10.1016/j.dci.2025.105497","url":null,"abstract":"<div><div><em>Morganella morganii</em> has emerged as a significant pathogen in aquaculture, yet its effects on largemouth bass (<em>Micropterus salmoides</em>) remain poorly understood. We isolated a pathogenic <em>M. morganii</em> strain (SH6) from diseased bass, caused over 76 % mortality in challenge tests with characteristic hemorrhaging, ascites, and hepatic lesions. Histopathological analysis revealed severe granulomatous inflammation and melanomacrophage centers in infected livers. Transcriptomic profiling identified 5799 differentially expressed genes, with significant enrichment in lipid metabolism pathways (fatty acid degradation) and immune responses (TLR signaling, cytokine-cytokine interactions). Notably, immune regulators (<em>il10</em>, <em>irak4</em>, <em>myd88</em>) were upregulated while drug metabolism enzymes (cytochrome P450) were suppressed. Antibiotic susceptibility testing indicated clinical potential for enrofloxacin and ciprofloxacin treatment, though resistance to azithromycin was observed. These findings demonstrate <em>M. morganii</em>'s significant threat to bass aquaculture through metabolic disruption and immune function, meanwhile identifying potential treatment options.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105497"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145328390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing probiotic properties of Lactiplantibacillus plantarum and Enterococcus faecalis in Cornu aspersum animal model","authors":"Kalodoti Avgousti ,&nbsp;Esmeralda Dushku ,&nbsp;Anna Spyropoulou ,&nbsp;Charalampos Kotzamanidis ,&nbsp;Alexandra Staikou ,&nbsp;Minas Yiangou","doi":"10.1016/j.dci.2025.105481","DOIUrl":"10.1016/j.dci.2025.105481","url":null,"abstract":"<div><div>This study explores the probiotic potential, immunomodulatory capacity, and safety of <em>Lactiplantibacillus plantarum</em> and <em>Enterococcus faecalis</em> strains isolated from the intestinal tract of the edible terrestrial snail <em>Cornu aspersum maxima</em>. Although host-microbe interactions are well studied in vertebrates, such research remains limited in invertebrates, particularly snails. To address this gap, 12 lactic acid bacteria strains were isolated and screened for tolerance to the defense mechanisms of snails and probiotic-associated traits, followed by machine learning (ML) predictions of immunomodulatory potential. According to results, 10 strains exhibited high tolerance to the external and internal defense mechanisms of snails (pedal and gastric mucus, gastric juices, low gut pH) in association with increased autoaggregation and hydrophobicity values and were predicted to have 100 % probability of eliciting immunomodulatory activity <em>in vivo</em>. Five strains, the <em>L. plantarum</em> Spp1 and Spp11 and <em>E. faecalis</em> Spp3, Spp8, Spp19, were selected for <em>in vivo</em> evaluation. Strain-specific immune responses were observed, with some strains mainly induced cellular immune responses, such as chemotaxis and phagocytic activity of hemocytes, while others also induced humoral responses. However, safety evaluations revealed that certain <em>E. faecalis</em> strains exhibited antimicrobial resistance or induced inflammatory reactions. Only two strains, the <em>L. plantarum</em> Spp11 and <em>E. faecalis</em> Spp19, were validated as safe and effective immunomodulatory probiotics <em>in vivo</em>. Overall, this study provides a comprehensive comparative analysis of the functionality of probiotic <em>Lactiplantibacillus</em> and <em>Enterococcus</em> strains in snails. These findings advance our understanding of snail-microbe symbiosis, particularly in the context of host-probiotic interactions, and support the use of <em>C. aspersum</em> as a valuable invertebrate model for probiotic research.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105481"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis the Astakine induced proliferation of hemocytes to regulate anti-bacterial immunity in Scylla paramamosain","authors":"Zhijuan Liu,&nbsp;Xinqi Liao,&nbsp;Xiaojun Zhong,&nbsp;Menghua Yang,&nbsp;Xiujuan Zhou","doi":"10.1016/j.dci.2025.105495","DOIUrl":"10.1016/j.dci.2025.105495","url":null,"abstract":"<div><div><em>Vibrio alginolyticus</em> presents a significant threat to the aquaculture sector of crustaceans, specifically <em>Scylla paramamosain</em>. Hemocyte proliferation is critical for the host's ongoing defense against bacterial pathogens and the maintenance of immune homeostasis. However, the molecular pathways governing hemocyte proliferation and immune responses in crustaceans are not fully elucidated. Building on our prior study, this study examines the effects of <em>V. alginolyticus</em> infection on immune tissue integrity and intestinal microbiota composition in <em>S. paramamosain</em>, with a focus on the role of the hemocyte proliferation regulator Astakine in host immune defense. Our results indicate that <em>V. alginolyticus</em> infection induces substantial histopathological injury to the hepatopancreas, gills, and intestinal tissues. Additionally, <em>V. alginolyticus</em> infection significantly modulates the composition and diversity of the intestinal microbiota. Astakine inhibits <em>V. alginolyticus</em> replication both in <em>vivo</em> and <em>in vitro</em>, while enhancing hemocyte proliferation, which promotes cell proliferation by accelerating G1/S cell cycle progression, and stimulating the secretion of antimicrobial peptides such as crustin and ALFs. Transcriptomic profiling reveals that Astakine activates cell proliferation signaling pathways, suppresses apoptosis pathways, and up-regulates immune-related genes including <em>jak</em>, <em>stat</em>, <em>ALF</em>, <em>Hsp90</em>, and <em>PI3K</em>, while down-regulating apoptosis-associated gene <em>caspase-2.</em> This study offers new insights into the molecular mechanisms by which crustacean hemocyte proliferation contributes to antibacterial immunity.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105495"},"PeriodicalIF":2.4,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145312583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}