Developmental and comparative immunology最新文献

{"title":"WTAP, a conserved m6A writer, can promote the antiviral immunity of Miichthys miiuy","authors":"Hanfu Gong ,&nbsp;Wenxin Li ,&nbsp;Shang Geng ,&nbsp;Tianjun Xu ,&nbsp;Yuena Sun","doi":"10.1016/j.dci.2024.105310","DOIUrl":"10.1016/j.dci.2024.105310","url":null,"abstract":"<div><div>N6-methyladenosine (m⁶A) is one of the most prevalent modifications found in eukaryotic mRNA and has been implicated in the regulation of cell proliferation, development, invasion, apoptosis, and immunity. In this study, we first conducted a structural and evolutionary analysis of Wilms' tumour 1-associating protein (WTAP) in vertebrates, and the results showed that WTAP in vertebrates is conserved particularly in mammals and fish. We subsequently investigated the involvement of WTAP in the antiviral immune response of fish and discovered that the expression of <em>Miichthys miiuy</em> (mmiWTAP) decreased in response to stimulation with <em>Siniperca chuatsi</em> rhabdovirus (SCRV) and poly(I:C). Immunofluorescence assays revealed that mmiWTAP was distributed in both the nucleus and the cytoplasm. Furthermore, overexpression of mmiWTAP enhanced the mRNA expression of MAVS and antiviral genes, thereby inhibiting SCRV replication. The beneficial effects of WTAP on MAVS and antiviral factors were disrupted upon introduction of cycloleucine, a methylation inhibitor, suggesting that the positive regulatory role of mmiWTAP in the antiviral immune response is reliant on its methyltransferase activity. These findings provide new insights into the involvement of m⁶A regulatory networks in fish antiviral immunity.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"162 ","pages":"Article 105310"},"PeriodicalIF":2.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomics reveals crowding stress inhibit the immune defense of the head kidney of the pearl gentian grouper juvenile through NF-κB signal pathway","authors":"Wenyang Li ,&nbsp;Ao Li ,&nbsp;Xianhong Zhang ,&nbsp;Fan Fei ,&nbsp;Xiaoqiang Gao ,&nbsp;Yingying Fang ,&nbsp;Shuquan Cao ,&nbsp;Hongjun Yang ,&nbsp;Wensheng Li ,&nbsp;Baoliang Liu","doi":"10.1016/j.dci.2024.105299","DOIUrl":"10.1016/j.dci.2024.105299","url":null,"abstract":"<div><div>Crowding stress is a significant welfare factor affecting aquatic animals in recirculating aquaculture systems. Little is known regarding the influence of prolonged crowding stress on the immunity of juvenile pearl gentian groupers. However, research exploring the potential mechanisms through which crowding stress affects fish immune function is limited. Therefore, this study aims to investigate the effect of crowding stress on the immune stress of the pearl gentian grouper juvenile (♀<em>Epinephelus fuscoguttatus</em> × ♂ <em>Epinephelus lanceolatus</em>) under prolonged conditions. We focused on the pearl gentian grouper juvenile and selected low- and high-density groups as the experimental breeding densities. Research shows that crowding stress increases the activities of alkaline acid plum and acid phosphatase, reduces the activities of lysozyme and immunoglobulin M content. RNA sequencing and comparative transcriptomic analyses were employed to explore changes in the gene expression of juvenile pearl gentian groupers subjected to crowding stress. Differential gene expression analyses between the low- and high-density groups identified 5777 unigenes that were differentially expressed following crowding stress, with 3216 and 2561 upregulated and downregulated, respectively. In the GO and KEGG enrichment analyses, many of the enriched signaling pathways related to genes were associated with immunity and oxidative stress. In addition, the combined analyses of enzyme activity and transcriptomics indicated that crowding stress suppressed the immune function of juvenile pearl gentian groupers, reducing their immune ability. Overall, these findings offer new insights into the molecular mechanisms underlying crowding stress tolerance in juvenile pearl gentian grouper, suggesting that the NF-κB pathway plays a crucial role in the immune response of the head kidney of the pearl gentian grouper to long-term crowding stress.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"162 ","pages":"Article 105299"},"PeriodicalIF":2.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ontogeny of the organized nasopharynx-associated lymphoid tissue in rainbow trout","authors":"Benjamin J. Garcia ,&nbsp;Narmin Musayeva ,&nbsp;Alexis Reyes ,&nbsp;Chrysler Martinez ,&nbsp;Yago Serra dos Santos ,&nbsp;Irene Salinas","doi":"10.1016/j.dci.2024.105298","DOIUrl":"10.1016/j.dci.2024.105298","url":null,"abstract":"<div><div>Understanding the ontogeny of teleost mucosa-associated lymphoid tissues (MALT) is critical for determining the earliest timepoint for effective mucosal vaccination of young fish. Here, we describe the developmental sequence that leads to the formation of an organized MALT structure in rainbow trout, the organized nasopharynx-associated lymphoid tissue (O-NALT). Control rainbow trout were sampled between 340 and 1860 degree days (DD) and routine histology and immunofluorescence staining were used to determine cellular changes in immune cells in the nasal cavity as well as O-NALT formation. We identified that O-NALT is first seeded by CD8α⁺ T cells at 700 DD followed by IgM⁺ B cells and <em>cd4-2b</em>⁺ cells at 1000 DD. Histomorphologically, trout O-NALT is fully formed at 1400 DD. Whole body gene expression analyses uncovered waves of <em>igmh</em>, <em>cd4-2b</em>, and <em>cd8a</em> expression that recapitulate the cellular seeding sequence of O-NALT by specific lymphocyte subsets. Our results indicate that 1) O-NALT formation results from a specific sequence of lymphocyte subset colonization pioneered by CD8α⁺ T cells and 2) the presence of the full O-NALT structure at 1400 DD may mark this timepoint as the earliest developmental stage at which mucosal vaccines can induce long lasting, specific immune responses.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"162 ","pages":"Article 105298"},"PeriodicalIF":2.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HSC70 functions as a negatively regulator in IFN signaling pathway via suppressing K63-linked ubiquitination of RIG-I in black carp","authors":"Jiaxin Fu ,&nbsp;Nianfeng chen ,&nbsp;Tian Qin ,&nbsp;Yixin Chen ,&nbsp;Ji Liu ,&nbsp;Hui Wu ,&nbsp;Jun Yan ,&nbsp;Jun Xiao ,&nbsp;Jun Zou ,&nbsp;Hao Feng","doi":"10.1016/j.dci.2024.105300","DOIUrl":"10.1016/j.dci.2024.105300","url":null,"abstract":"<div><div>Heat shock cognate 70 (HSC70), a highly conserved molecular chaperone in the heat shock protein 70 (HSP70) family, plays an essential role in maintaining the homeostasis of the cellular environment. Furthermore, although previous studies have investigated potential function of HSC70 in innate antiviral immunity, further research is still required to fully elucidate its role. In this study, we cloned and characterized the HSC70 homolog gene from black carp (<em>Mylopharyngodon piceus</em>), which consists of 1950 nucleotides encoding 650 amino acids, migrates at approximately 71 kDa on SDS-PAGE, and is distributed in the cytoplasm. In response to different stimuli (SVCV, poly (I:C) and LPS), the transcription level of black carp HSC70 (bcHSC70) all increased to a certain extent. Luciferase reporter assay demonstrated that co-transfected bcHSC70 obviously reduced activity of interferon (IFN) promoters mediated by most factors in the RLRs pathway, and further qRT-PCR and plaque assay indicated that co-transfection of bcHSC70 with bcRIG-I decreased the bcRIG-I-mediated IFN transcription and antiviral ability resisting spring viremia of carp virus (SVCV), whereas knockdown of bcHSC70 improves the host cellular antiviral activity. Noteworthily, co-immunoprecipitation (co-IP) assay and immunofluorescence (IF) assay confirmed bcHSC70 interacts with bcRIG-I, and weaken K63-linked polyubiquitination of bcRIG-I. In summary, our study revealed that HSC70 negatively regulates IFN signaling pathway through impairing K63-linked ubiquitination of RIG-I in black carp, which provides an important basis for exploring innate immune regulatory mechanisms in teleost fish.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"162 ","pages":"Article 105300"},"PeriodicalIF":2.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of Peroxiredoxin-5 (PRDX5) in starry flounder (Platichthys stellatus): Molecular features and expression analysis","authors":"Min-Young Sohn ,&nbsp;Kwang-Min Choi ,&nbsp;Ji-Min Jeong ,&nbsp;Gyoungsik Kang ,&nbsp;Won-Sik Woo ,&nbsp;Kyung-Ho Kim ,&nbsp;Ha-Jeong Son ,&nbsp;Hye-Seong Kim ,&nbsp;Jun-ichi Hikima ,&nbsp;Chan-Il Park","doi":"10.1016/j.dci.2024.105291","DOIUrl":"10.1016/j.dci.2024.105291","url":null,"abstract":"<div><div>In this study, the Peroxiredoxin-5 (PRDX5) gene from starry flounder (<em>Platichthys stellatus</em>, PsPRDX5) was identified through next-generation sequencing (NGS) and verified via cloning and sequencing. The predicted amino acid sequence, derived from the cDNA, was confirmed by multiple sequence alignment and phylogenetic analysis, establishing its homology with related sequences. PsPRDX5 mRNA was expressed across all examined tissues in healthy starry flounder, with distinct tissue-specific expression profiles. Following artificial infection with viral hemorrhagic septicemia virus (VHSV) and <em>Streptococcus parauberis</em> PH0710, significant alterations in PsPRDX5 mRNA expression were observed. VHSV infection resulted in a pronounced downregulation of PsPRDX5 in most major tissues, except for a significant upregulation in the intestine at 7 days post-infection. Conversely, infection with <em>S. parauberis</em> PH0710 led to a significant upregulation of PsPRDX5 in nearly all tissues. These results suggest that the differential expression of PsPRDX5 in response to VHSV and <em>S. parauberis</em> PH0710 reflects pathogen-specific immune and antioxidant defense mechanisms, highlighting the role of PsPRDX5 in the immune response of starry flounder to both viral and bacterial infections.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"162 ","pages":"Article 105291"},"PeriodicalIF":2.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Caspase-8 promotes innate immunity in the Chinese mitten crab by regulating the expression of antimicrobial peptides and apoptosis in hemocyte","authors":"Guangyu Wang ,&nbsp;Guoqing Shen ,&nbsp;Chaohui Xu ,&nbsp;Yanan Guo ,&nbsp;Wen Zhang ,&nbsp;Qun Wang ,&nbsp;Youting Zhu","doi":"10.1016/j.dci.2024.105308","DOIUrl":"10.1016/j.dci.2024.105308","url":null,"abstract":"<div><div>In mammals, caspase-8 primarily functions as an initiator caspase that regulates apoptosis, while in <em>Drosophila</em>, the caspase-8 ortholog DREDD not only induces apoptosis during development but also regulates antimicrobial peptides (AMPs) expression during Gram-negative bacterial infection-induced immune responses. However, the immune-related function of caspase-8 in the crustacean remains unknown. In the present study, the open reading frame of <em>EsCaspase-8</em> was cloned from the Chinese mitten crab (<em>Eriocheir sinensis</em>). The deduced <em>Es</em>Caspase-8 protein sequence contained only one death effector domain (DED) and a cysteine aspartase cysteine structural domain. The <em>EsCaspase-8</em> expression was significantly induced after 6 h of <em>Vibrio parahaemolyticus</em> infection and continued to 24 h in hemocyte. Knocking down <em>EsCaspase-8</em> expression in hemocytes significantly inhibited Relish's nuclear translocation and suppressed the expression of AMPs, including <em>Crustin 1</em>, <em>Crustin 2</em>, <em>Lysosome,</em> and <em>double WAP domain</em>, after <em>V</em>. <em>parahaemolyticus</em> infection<em>.</em> Furthermore, the knockdown of <em>EsCaspase-8 in vivo</em> significantly inhibited hemocyte apoptosis post-bacterial infection. These results demonstrated that <em>Es</em>Caspase-8 can promote antibacterial activities by regulating the expression of AMPs through activation of Relish nuclear translocation in Chinese mitten crabs, thus acting as a critical positive regulator in innate immunity. In addition, <em>Es</em>Caspase-8 also has the function of inducing hemocyte apoptosis. These findings expand our understanding of the molecular mechanisms underlying crustacean immune responses and provide a foundation for future research to improve disease resistance.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"162 ","pages":"Article 105308"},"PeriodicalIF":2.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infectious myonecrosis virus (IMNV) induces upregulation of RNAi-related genes in white shrimp Penaeus vannamei","authors":"Rubens Galdino Feijó ,&nbsp;Jhonatas Teixeira Viana ,&nbsp;Rodrigo Maggioni ,&nbsp;Luis Fernando Marins","doi":"10.1016/j.dci.2024.105296","DOIUrl":"10.1016/j.dci.2024.105296","url":null,"abstract":"<div><div>Infectious myonecrosis virus (IMNV) still causes significant economic and social losses in American and Asian shrimp farming. In this work, we investigated the transcription patterns of <em>Sid-1</em>, <em>Dicer-2</em> and <em>Argonaute-2</em> genes from the RNAi mechanism in <em>Penaeus vannamei</em> naturally infected with IMNV, and injected with inoculum containing 1.02 × 10⁵, 1.02 × 10⁴ or 1.02 × 10³ IMNV copies‧μL⁻¹. We observed that infection with increasing IMNV concentrations affected the transcription levels of these key genes. However, the viral load did not decrease during the experiment. We suggest that changes in <em>Sid-</em>1 mRNA expression could be used as marker of viral replication for evaluating sanitary status in <em>P. vannamei</em> farming.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"162 ","pages":"Article 105296"},"PeriodicalIF":2.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142779269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CgmiR307 involved in the regulation of Nrf2-dependent oxidative response in the Pacific oyster Crassostrea gigas under high-temperature stress","authors":"Keli Zhou ,&nbsp;Lei Gao ,&nbsp;Pingan Ge ,&nbsp;Ling Wang ,&nbsp;Lu Liu ,&nbsp;Jiayu Ye ,&nbsp;Hairu Xu ,&nbsp;Lingling Wang ,&nbsp;Linsheng Song","doi":"10.1016/j.dci.2024.105306","DOIUrl":"10.1016/j.dci.2024.105306","url":null,"abstract":"<div><div>miRNA, a type of endogenous small non-coding RNA, is involved in the response to various environmental stresses through post-transcriptional regulation. In the present study, the role of <em>Cg</em>miR307 in the regulation of oxidative response under high-temperature stress by targeting <em>Cg</em>Nrf2 was investigated in the Pacific oyster <em>Crassostrea gigas</em>. The binding site of <em>Cg</em>miR307 were predicted at 1799–1818 bp in the 3′-UTR of <em>Cg</em>Nrf2, and the binding activity of <em>Cg</em>miR307 with the mRNA of <em>Cg</em>Nrf2 was further proved by the dual-luciferase reporter assay. The expression levels of <em>Cg</em>miR307 and <em>Cg</em>Nrf2 in gill were significantly higher than in other tissues, and exhibited significant fluctuations and variations after exposure to 28 °C. There was a significant reduction in the expression levels of <em>Cg</em>SOD, and <em>Cg</em>CAT in gill, as well as the activities of SOD, CAT, and T-AOC, while ROS and MDA contents significantly increased in <em>Cg</em>Nrf2-RNAi oysters. After <em>Cg</em>miR307 agomir injection and high-temperature stress, the expression levels of <em>Cg</em>Nrf2, <em>Cg</em>SOD and <em>Cg</em>CAT in gill, the activities of SOD and CAT and T-AOC decreased significantly, while ROS and MDA content significantly increased. After <em>Cg</em>miR307 antagomir injection and high-temperature stress, the changes in the parameters of oxidative response shown exactly the opposite trend. These results demonstrated that <em>Cg</em>miR307 was involved in the regulation of oxidative response by inhibiting the mRNA expression of <em>Cg</em>Nrf2 under high-temperature stress.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"162 ","pages":"Article 105306"},"PeriodicalIF":2.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142876537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular dynamics and spatial response of proliferation and apoptosis in wound healing and early intestinal regeneration of sea cucumber Apostichopus japonicus","authors":"Fang Su ,&nbsp;Igor Yu. Dolmatov ,&nbsp;Wei Cui ,&nbsp;Hongsheng Yang ,&nbsp;Lina Sun","doi":"10.1016/j.dci.2024.105297","DOIUrl":"10.1016/j.dci.2024.105297","url":null,"abstract":"<div><div>The sea cucumber, <em>Apostichopus japonicus</em>, exhibits significant regenerative capabilities. To ensure survival and reduce metabolic costs under adverse conditions, <em>A</em>. <em>japonicus</em> can expel intestine, respiratory trees and other internal organs. It takes only 14 days to regenerate a fully connected, lumen-containing intestine. Despite numerous reports characterizing the cellular events in intestinal regeneration, limited investigation has been conducted on the molecular events that occur during wound healing and the initial stages of regeneration after evisceration. Here, we identified differentially expressed genes (DEGs) during wound healing (6 h post-evisceration, Aj6hpe) and early intestinal regeneration (Aj1dpe, Aj3dpe, Aj7dpe). Cell proliferation and apoptosis were detected by EdU and TUNEL assays, respectively. Results demonstrated that calcium ion and neuroactive ligand-receptor interaction were involved in the transmission of injury signals from evisceration to Aj1dpe. The main events occurring in the wound healing and early regeneration process were autophagy, apoptosis, dedifferentiation, migration and shutdown of feeding. Cell proliferation was primarily observed during the lumen formation stage. Maximal number of apoptotic cells were found during wound healing stage (6 hpe - 1 dpe). Consequently, the immune response is mainly mobilized by neural regulation after evisceration. Our findings bridge the gap between evisceration and regeneration, illuminating the molecular events that mediate damage response and initiate regeneration. This study significantly advances our understanding of the mechanisms underlying intestinal regeneration.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"162 ","pages":"Article 105297"},"PeriodicalIF":2.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel digestive protease chymotrypsin-like serine contributes to anti-BmNPV activity in silkworm (Bombyx mori)","authors":"Wei-Wei Kong ,&nbsp;Yu-Liang Yan ,&nbsp;Cai-Ping Hou ,&nbsp;Tao Hong ,&nbsp;Yi-Sheng Wang ,&nbsp;Xin Xu ,&nbsp;Shi-Huo Liu ,&nbsp;Jia-Ping Xu","doi":"10.1016/j.dci.2024.105301","DOIUrl":"10.1016/j.dci.2024.105301","url":null,"abstract":"<div><div>Serine proteases (SPs) are important proteases in the digestive system of lepidopteran insects. They play important roles in protein digestion, coagulation, signal transduction, hormone activation, inflammation and development. Blood-borne pyosis caused by <em>Bombyx mori</em> nuclear polyhedrosis virus (BmNPV) has caused serious harm to sericulture. At present, the scientific problems of BmNPV infection and silkworm resistance to BmNPV infection have been the focus of many scientists, but the molecular mechanism needs further research and exploration. Based on the results of label-free quantitative protein proteomics of the midgut digestive juice of different resistant strains in our laboratory, we successfully screened a differentially expressed candidate protein (DEP), <em>B. mori</em> chymotrypsin-like serine protease (BmCLSP), and comprehensively analyzed the biological characteristics and anti-BmNPV function of BmCLSP. The open reading frame (ORF) of <em>BmCLSP</em> is 891 bp, encoding 296 amino acid residues. The analysis of the domain structure showed that there was a signal peptide and a trypsin-like serine protease domain, Tryp_SPC, in the BmCLSP protein. Semi-quantitative and real-time fluorescence quantitative PCR analysis showed that the <em>BmCLSP</em> gene was highly expressed in the fifth instar larvae of silkworm, and specifically expressed in the midgut. The expression level of <em>BmCLSP</em> in the BmNPV resistant strain A35 was higher than that in the sensitive strain P50. Virus amplification analysis showed that the relative expression level of <em>VP39</em> was significantly lower than that of the control group after infection of silkworm larvae and BmN cells with BmNPV treated with recombinant BmCLSP at an appropriate concentration. Furthermore, our overexpression of BmCLSP in BmN cells significantly inhibited the expansion of BmNPV. In summary, the results of this study indicate that BmCLSP has anti-BmNPV activity in silkworm, and can significantly inhibit the proliferation of BmNPV in silkworm. It offers a promising avenue for silkworm anti-virus breeding.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"162 ","pages":"Article 105301"},"PeriodicalIF":2.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}