Developmental and comparative immunology最新文献

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Limited early transcriptome-wide mucosal response to the bacterial pathogen Yersinia ruckeri in Atlantic salmon (Salmo salar) alevins 大西洋鲑鱼(Salmo salar) alevins中有限的早期转录组全粘膜对细菌病原体拉克氏耶尔森菌的反应
IF 2.4 3区 农林科学
Developmental and comparative immunology Pub Date : 2025-08-27 DOI: 10.1016/j.dci.2025.105449
Carlo C. Lazado , Marianne Iversen , Hanne Brenne , Torstein Tengs , Erik Burgerhout
{"title":"Limited early transcriptome-wide mucosal response to the bacterial pathogen Yersinia ruckeri in Atlantic salmon (Salmo salar) alevins","authors":"Carlo C. Lazado ,&nbsp;Marianne Iversen ,&nbsp;Hanne Brenne ,&nbsp;Torstein Tengs ,&nbsp;Erik Burgerhout","doi":"10.1016/j.dci.2025.105449","DOIUrl":"10.1016/j.dci.2025.105449","url":null,"abstract":"<div><div>Atlantic salmon larvae possess immune structures, effector cells, and molecules, and their mucosal organs serve as critical barriers against pathogens. But despite these defences, fish larvae show limited immune response capabilities, and the early transcriptomic responses of different mucosal surfaces remain poorly understood. Therefore, this study investigated the transcriptomic landscape of Atlantic salmon (<em>Salmo salar</em>) alevins exposed to the pathogen <em>Yersinia ruckeri</em>, analysing three mucosal organs (skin, gills, and tongue) over 72 h post-exposure. RNA-Seq analysis revealed distinct basal transcriptomic profiles among organs, with gill and tongue showing similarities possibly due to anatomical proximity. Following pathogen exposure, organ-specific immune responses emerged, though relatively subdued in magnitude. The tongue showed peak response at 24 h with 117 differentially expressed genes (DEGs), while skin demonstrated the strongest response at 72 h with 483 DEGs. Notably, 18 shared DEGs between these responses encoded acute-phase proteins, indicating activated mucosal inflammatory responses. The skin exhibited 64 enriched Biological Processes (BP) related to metabolism, gene regulation, and innate immunity at 72 h. In contrast, 33 BP terms enriched in the tongue at 24 h were associated with the regulation of micro- and macromolecules, including lipids, proteins, glucose, and organic acids. Notably, 11 BP terms were common between the two organs, primarily linked to blood coagulation, wound healing, and hemostasis. These findings illuminate host-pathogen interactions at mucosal sites during early development and provide new insights into the temporal dynamics of mucosal immunity to <em>Y. ruckeri</em> in salmon alevins. Furthermore, this study provides the first evidence that the teleost tongue contributes to host immunity.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"170 ","pages":"Article 105449"},"PeriodicalIF":2.4,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144911952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
MicroRNA-301a directly targets IL-4/13A to negatively regulate Th2 immune responses in large yellow croaker (Larimichthys crocea) MicroRNA-301a直接靶向IL-4/13A负向调控大黄鱼Th2免疫应答
IF 2.4 3区 农林科学
Developmental and comparative immunology Pub Date : 2025-08-26 DOI: 10.1016/j.dci.2025.105445
Huazhi Chen , Jiamei Liu , Yangfan Xiao , Wenxing Li , Xinhua Chen , Yinnan Mu
{"title":"MicroRNA-301a directly targets IL-4/13A to negatively regulate Th2 immune responses in large yellow croaker (Larimichthys crocea)","authors":"Huazhi Chen ,&nbsp;Jiamei Liu ,&nbsp;Yangfan Xiao ,&nbsp;Wenxing Li ,&nbsp;Xinhua Chen ,&nbsp;Yinnan Mu","doi":"10.1016/j.dci.2025.105445","DOIUrl":"10.1016/j.dci.2025.105445","url":null,"abstract":"<div><div>Interleukin-4 (IL-4) and IL-13, predominantly secreted by T helper type 2 (Th2) cells, are pivotal cytokines involved in the Th2 response via the MAPK and mTOR signaling pathways. MicroRNAs (miRNAs) modulate various biological processes by inhibiting mRNA translation or facilitating mRNA degradation. However, research on miRNAs targeting IL-4 and IL-13 and their associated downstream signaling molecules remains limited. In this study, the miR-301a was identified for the first time as a potential regulator of IL-4/13A in <em>Larimichthys crocea</em> (<em>Lc</em>) through comprehensive analyses of miRNA-mRNA transcriptomes. The <em>Lc</em>pre-miR-301a comprises 86 nucleotides, including a 21-bp mature miRNA sequence. Secondary structure prediction showed that <em>Lc</em>pre-miR-301a can form a classic hairpin structure. Multiple sequence alignment revealed that the mature <em>Lc</em>miR-301a is highly conserved across fish, mammals, amphibians, and birds. The miR-301a was demonstrated to directly bind to the 3′-UTR of <em>Lc</em>IL-4/13A. Upon induction with phytohemagglutinin (PHA), the expression levels of <em>Lc</em>IL-4/13A were significantly upregulated, leading to the activation of T cell proliferation and differentiation, as well as B cell proliferation in primary head kidney leukocytes (PKLs). Conversely, the overexpression of miR-301a in PKLs significantly inhibited the PHA-induced upregulation of <em>Lc</em>IL-4/13A expression level. Additionally, the stimulatory effects of PHA on T cells and B cells were diminished by the overexpression of <em>Lc</em>miR-301a. Moreover, after transfection with miR-301a mimics and subsequent induction with PHA, the phosphorylation levels of MEK1/2 and 4E-BP1, two critical signaling molecules within the MAPK and mTOR pathways, were significantly reduced in PKLs compared to induction with PHA alone. These findings suggest that miR-301a plays a crucial role in negatively regulating the Th2 immune response by targeting <em>Lc</em>IL-4/13A. This study enhances our understanding of the mechanisms underlying miRNA regulation in the adaptive immune response of fish.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"170 ","pages":"Article 105445"},"PeriodicalIF":2.4,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144902255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cd36 deficiency enhances the immune defence against Grass Carp Reovirus in zebrafish 缺乏Cd36增强斑马鱼对草鱼呼肠孤病毒的免疫防御
IF 2.4 3区 农林科学
Developmental and comparative immunology Pub Date : 2025-08-25 DOI: 10.1016/j.dci.2025.105446
Jing Gao , Wenjin Li , Meng Li , Guangdong Ji , Zhenhui Liu
{"title":"Cd36 deficiency enhances the immune defence against Grass Carp Reovirus in zebrafish","authors":"Jing Gao ,&nbsp;Wenjin Li ,&nbsp;Meng Li ,&nbsp;Guangdong Ji ,&nbsp;Zhenhui Liu","doi":"10.1016/j.dci.2025.105446","DOIUrl":"10.1016/j.dci.2025.105446","url":null,"abstract":"<div><div>CD36, a single-chain transmembrane glycoprotein belonging to the class B scavenger receptor family, remains enigmatic in terms of its antiviral immune function, with some conflicting conclusions. This study aims to elucidate the role of Cd36 in antiviral immunity using zebrafish as a model organism. We generated <em>cd36</em><sup><em>−/−</em></sup> zebrafish mutants using CRISPR/Cas9 technology and challenged them with Grass Carp Reovirus (GCRV), a dsRNA virus. Compared to wild-type (WT) zebrafish, <em>cd36</em><sup><em>−/−</em></sup> zebrafish exhibited stronger antiviral immunity, reduced viral load, and lower mortality. Transcriptome sequencing analysis of liver tissue revealed that the differentially expressed genes (DEGs) between WT and <em>cd36</em><sup><em>−/−</em></sup> zebrafish were primarily enriched in metabolic pathways, such as glycan biosynthesis and fatty acid metabolism. This suggests that Cd36 may modulate antiviral immune responses by influencing metabolic activities. Our findings provide a foundation for further investigations into the functional and molecular mechanisms of Cd36 in antiviral immunity in fish.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"170 ","pages":"Article 105446"},"PeriodicalIF":2.4,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144902256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular epidemiology of acute hepatopancreatic necrosis disease: A review 急性肝胰腺坏死病的分子流行病学研究进展
IF 2.4 3区 农林科学
Developmental and comparative immunology Pub Date : 2025-08-18 DOI: 10.1016/j.dci.2025.105444
Tram-Anh Bui-Nguyen , Tuan-Binh Huynh , Hieu Tran-Van
{"title":"Molecular epidemiology of acute hepatopancreatic necrosis disease: A review","authors":"Tram-Anh Bui-Nguyen ,&nbsp;Tuan-Binh Huynh ,&nbsp;Hieu Tran-Van","doi":"10.1016/j.dci.2025.105444","DOIUrl":"10.1016/j.dci.2025.105444","url":null,"abstract":"<div><div>Acute hepatopancreatic necrosis disease (AHPND) is one of the major shrimp diseases worldwide which affects global economy up to 44 billion USD from 2010 to 2016. The causative agent of AHPND is the binary toxin PirAB, a toxin that causes sloughing effect on shrimp hepatopancreatic cells. This toxin is encoded by <em>pirAB</em><sup><em>vp</em></sup> gene located within a 5.5-kb composite transposon <em>Tn</em>6264, on a ∼70-kb plasmid pVA carried by <em>Vibrio parahaemolyticus</em>. Up to date, the pathogenesis and epidemiological links between AHPND-causing strains are still unclear. Therefore, this review aims to collect achieved results about the distribution, origin, transmission, and antibiotic resistance status of AHPND-causing strains, the molecular mechanism of PirAB toxin, and the mobile genetic elements that promote the spread of AHPND to provide valuable insights for future studies. Phylogenetic studies on AHPND reveal its evolutionary history, transmission routes, and genetic variations, with findings suggesting diverse origins of AHPND strains across different regions, facilitated by horizontal gene transfer and adaptation mechanisms in <em>V. parahaemolyticus</em> populations. Antimicrobial resistance profiles of AHPND-causing strains are also diverse and prevalent, particularly in Vietnam, South Korea, and Thailand, encompassing antibiotics like ampicillin, amoxicillin, sulfadiazine sodium, streptomycin, colistin, cefalexin, erythromycin, ceftazidime, and neomycin, raising concerns regarding multidrug resistance. PirAB toxin might function through the pore-forming activity of PirB<sup>vp</sup> and the receptor-binding activity of PirA<sup>vp</sup>, as predicted by Cry toxin model, while its expression is regulated by the quorum sensing system in <em>V. parahaemolyticus</em>. The pVA plasmid and the composite transposon <em>Tn</em>6264 both facilitates the dissemination of AHPND-causing strains, while the evolutionary mechanisms of these elements have not been widely understood. Transcriptomic and metabolomic studies also identify numerous differentially expressed genes in shrimp infected by AHPND-causing V. parahaemolyticus, and its immunity is also dependent on developmental stage and gut microbiota.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"170 ","pages":"Article 105444"},"PeriodicalIF":2.4,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144889044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Channel catfish TCRβ and IGH receptor repertoires - Public clonotypes complement a highly diverse repertoire 通道鲶鱼TCRβ和IGH受体库-公共克隆型补充了高度多样化的库。
IF 2.4 3区 农林科学
Developmental and comparative immunology Pub Date : 2025-08-13 DOI: 10.1016/j.dci.2025.105443
R. Craig Findly , Ryan P. Sweeney , Frank D. Niagro , Alvin C. Camus , Harry W. Dickerson
{"title":"Channel catfish TCRβ and IGH receptor repertoires - Public clonotypes complement a highly diverse repertoire","authors":"R. Craig Findly ,&nbsp;Ryan P. Sweeney ,&nbsp;Frank D. Niagro ,&nbsp;Alvin C. Camus ,&nbsp;Harry W. Dickerson","doi":"10.1016/j.dci.2025.105443","DOIUrl":"10.1016/j.dci.2025.105443","url":null,"abstract":"<div><div>The sequence diversity encompassed in the repertoires of T and B cell receptors is fundamental to their roles in adaptive immunity. The sequence diversity in the expressed complementarity determining region three of both the TCRβ and IGH receptors of the channel catfish, <em>Ictalurus punctatus</em>, was compared by high-throughput sequencing of two cDNA libraries constructed using samples isolated from eleven fish over 28 months. Samples were collected both before and after infection with the parasitic protozoan <em>Ichthyophthirius multifiliis</em>, or vaccination with a protective <em>I. multifiliis</em> antigen. Samples were taken from blood and fin before and at 8 and 15 months after treatment, and from blood, skin, spleen and head kidney at 28 months after treatment. The libraries were not sequenced to saturation. In both the TCRβ and IGH repertoires, approximately 70 % of clonotypes were detected only once. Two TCRβ and eight IGH public clonotypes were widely distributed over the entire 28 months. The cDNA sequence of each public clonotype was identical in all fish, suggesting that the rearranged VDJ gene coding for it is in the germline. Rearranged IGH VDJ genes from three IGH V gene families were shown to be in the germline. Seven sterile TCRβ sequences, which contained a spliced JC gene sequence but lacked V and D gene sequences, comprised most copies of TCRβ cDNAs. The TCRβ and IGH repertoires are composed of a traditional repertoire of highly diverse, primarily unique clonotypes, and a second repertoire with a few constant, public clonotypes.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"170 ","pages":"Article 105443"},"PeriodicalIF":2.4,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144859045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Whole-genome identification and expression profiling of TRPs family in Chinese mitten crab Eriocheir sinensis 中华绒螯蟹TRPs家族的全基因组鉴定及表达谱分析
IF 2.4 3区 农林科学
Developmental and comparative immunology Pub Date : 2025-08-13 DOI: 10.1016/j.dci.2025.105442
Xirui Zheng , Maolei Wei , Yanan Gao , Wanzhen Ma , Xugan Wu
{"title":"Whole-genome identification and expression profiling of TRPs family in Chinese mitten crab Eriocheir sinensis","authors":"Xirui Zheng ,&nbsp;Maolei Wei ,&nbsp;Yanan Gao ,&nbsp;Wanzhen Ma ,&nbsp;Xugan Wu","doi":"10.1016/j.dci.2025.105442","DOIUrl":"10.1016/j.dci.2025.105442","url":null,"abstract":"<div><div>Although transient receptor potential (TRP) proteins are critical to diverse physiological processes, their characteristics in arthropods, especially in the Chinese mitten crab (<em>Eriocheir sinensis</em>), remain poorly understood. The present study aimed to elucidate the evolutionary history and physiological functions of <em>TRP</em> genes (<em>TRPs</em>) in <em>E. sinensis</em> through a combination of phylogenetic analyses and transcriptomic approaches. The evolutionary analysis revealed a significant expansion of the <em>TRPA</em> subfamily in arthropods. Sequence analysis and codon usage bias assessment further indicated that <em>TRPs</em> exhibit a certain degree of conservation between <em>E. sinensis</em> and its closely related species, suggesting they are likely subject to evolutionary selective pressures. To investigate the physiological roles of <em>TRPs</em> in <em>E. sinensis</em>, we conducted transcriptomic analysis of <em>TRPs</em> across different tissues, molting stages of the hepatopancreas, and ovarian developmental stages. The results showed that <em>TRPA</em> genes were highly expressed in the gills, hindgut, and midgut, suggesting a potential involvement in immune response. The significant overexpression of <em>TRP</em> and <em>TRPL</em> in the eyestalk implied their possible role in phototransduction. High expression of <em>TRPs</em> was observed in the hepatopancreas during various molting stages. Additionally, <em>TRPC</em> genes were highly expressed at different ovarian developmental stages. Overall, this study revealed the evolutionary dynamics of <em>TRPs</em> in <em>E. sinensis</em> and provided preliminary evidence for their potential regulatory roles in tissue function, molting, and ovarian maturation, with further experimental validation required in the future.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"170 ","pages":"Article 105442"},"PeriodicalIF":2.4,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144858182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A big anti-lipopolysaccharide factor showing sexual dimorphism is involved in anti-WSSV immunity in crayfish 一种具有两性二态性的大抗脂多糖因子参与了小龙虾抗wssv免疫
IF 2.4 3区 农林科学
Developmental and comparative immunology Pub Date : 2025-08-12 DOI: 10.1016/j.dci.2025.105441
Liangmin Zheng , Hao Ji , Zhaoqian Zhang , Binyan Wang , Zhiqiang Xu , Qian Ren , Xin Huang
{"title":"A big anti-lipopolysaccharide factor showing sexual dimorphism is involved in anti-WSSV immunity in crayfish","authors":"Liangmin Zheng ,&nbsp;Hao Ji ,&nbsp;Zhaoqian Zhang ,&nbsp;Binyan Wang ,&nbsp;Zhiqiang Xu ,&nbsp;Qian Ren ,&nbsp;Xin Huang","doi":"10.1016/j.dci.2025.105441","DOIUrl":"10.1016/j.dci.2025.105441","url":null,"abstract":"<div><div>Anti-lipopolysaccharide factor (ALF) is an important active substance in crustacean innate immunity with broad-spectrum antimicrobial and antiviral effects. In this study, we identified a big ALF from <em>Procambarus clarkii</em>, named <em>PcbALF</em>. The full-length cDNAs of two <em>PcbALF</em> isoforms (<em>PcbALF1</em> and <em>PcbALF2</em>) were 2619 bp and 2142 bp with an open reading frame of 1074 bp and 1317 bp that encodes 357 and 438 amino acids, respectively. Intron retention of <em>PcbALF1</em> resulted in the loss of the DUF3254 domain. <em>PcbALF1</em> and <em>PcbALF2</em> were widely distributed in multiple tissues and their expression in different tissues exhibits sexual dimorphism. Further study found that <em>PcbALF2</em> expression levels were up-regulated in the intestine and gills of male and female <em>P. clarkii</em> after stimulation with white spot syndrome virus (WSSV) from 0 to 48 h. Furthermore, RNA interference analysis showed that the knockdown of <em>PcbALF2</em> inhibited <em>PcbALF2</em> transcription and promoted <em>VP28</em> expression during WSSV infection. Injection of rPcbALF with DUF3254 domain suppressed <em>VP28</em> expression, suggesting that PcbALF has a negative role in WSSV replication. Our study shows that <em>PcbALF</em> is involved in anti-WSSV immunity in <em>P. clarkii</em> and exhibits sexual dimorphism.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"170 ","pages":"Article 105441"},"PeriodicalIF":2.4,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144840916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unveiling the multifunctional role of CXCL family chemokines in teleost immunity: a functional study of CXCL3 in yellowtail clownfish (Amphiprion clarkii) 揭示CXCL家族趋化因子在硬骨鱼免疫中的多功能作用:CXCL3在黄尾小丑鱼(clarkii Amphiprion)中的功能研究
IF 2.4 3区 农林科学
Developmental and comparative immunology Pub Date : 2025-08-09 DOI: 10.1016/j.dci.2025.105440
J.D.H.E. Jayasinghe , Hanchang Sohn , Seongdo Lee , Seong Don Hwang , Qiang Wan , Jehee Lee
{"title":"Unveiling the multifunctional role of CXCL family chemokines in teleost immunity: a functional study of CXCL3 in yellowtail clownfish (Amphiprion clarkii)","authors":"J.D.H.E. Jayasinghe ,&nbsp;Hanchang Sohn ,&nbsp;Seongdo Lee ,&nbsp;Seong Don Hwang ,&nbsp;Qiang Wan ,&nbsp;Jehee Lee","doi":"10.1016/j.dci.2025.105440","DOIUrl":"10.1016/j.dci.2025.105440","url":null,"abstract":"<div><div>CXCL3, a chemokine with broad immunomodulatory roles, was molecularly and functionally characterized in yellowtail clownfish (<em>Amphiprion clarkii</em>). The identified <em>AcCXCL3</em> gene exhibited a tissue-specific expression pattern, with particularly high mRNA levels in the spleen and head kidney. Its expression was significantly upregulated in response to both viral and bacterial immune challenges, suggesting a strong inducible role in host defense. Functional assays using recombinant AcCXCL3 (rAcCXCL3) demonstrated its ability to regulate apoptosis by modulating <em>bcl-2</em> and <em>bax</em>, exerting significant antiviral activity against viral hemorrhagic septicemia virus (VHSV), and enhancing wound healing. Notably, rAcCXCL3 promoted M1-like macrophage polarization, indicating its potential in directing pro-inflammatory innate immune responses. Gene expression analyses further revealed that rAcCXCL3 influenced key signaling pathways, including NF-κB, JAK/STAT, MyD88, and MAPK, underscoring its role in immune regulation. Collectively, this study highlights the significant role of AcCXCL3 as a multifunctional chemokine in the intricate regulation of immunity and tissue homeostasis in yellowtail clownfish. These findings contribute fundamental knowledge to our understanding of chemokine functions within aquatic vertebrates and pave the way for future research exploring their potential in fish health and disease management.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"170 ","pages":"Article 105440"},"PeriodicalIF":2.4,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144820880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Butyrate increases ICAM-1 expression in primary bovine endothelial cells through MCT1 and histone deacetylase inhibition 丁酸盐通过MCT1和组蛋白去乙酰化酶抑制原代牛内皮细胞中ICAM-1的表达。
IF 2.4 3区 农林科学
Developmental and comparative immunology Pub Date : 2025-08-09 DOI: 10.1016/j.dci.2025.105439
Maria Daniella Carretta , Yonathan Barria , Nicole Gallardo , Pablo Alarcón , John Quiroga , Rafael Burgos , Anja Taubert , Carlos Hermosilla
{"title":"Butyrate increases ICAM-1 expression in primary bovine endothelial cells through MCT1 and histone deacetylase inhibition","authors":"Maria Daniella Carretta ,&nbsp;Yonathan Barria ,&nbsp;Nicole Gallardo ,&nbsp;Pablo Alarcón ,&nbsp;John Quiroga ,&nbsp;Rafael Burgos ,&nbsp;Anja Taubert ,&nbsp;Carlos Hermosilla","doi":"10.1016/j.dci.2025.105439","DOIUrl":"10.1016/j.dci.2025.105439","url":null,"abstract":"<div><div>Endothelial cells play a crucial role in the migration of leukocytes to sites of infection or inflammation, mainly through the expression of adhesion molecules. Butyrate, a short-chain fatty acid (SCFA) produced by the gut microbiota, exerts anti-inflammatory and pro-inflammatory effects by regulating these molecules. This discrepancy may be related to the activation or inhibition of different targets, such as G-protein-coupled receptors and histone deacetylases. This study aimed to characterize the effect of butyrate on the expression of endothelial adhesion molecule ICAM-1 and its underlying mechanism in primary bovine umbilical vein endothelial cells (BUVEC). We hypothesized that butyrate increases endothelial ICAM-1 expression, and this effect is associated with the activation of the free fatty acid receptor 2 (FFAR2) or hydroxycarboxylic acid receptor 2 (HCA2) and/or inhibition of histone deacetylase 3 (HDAC3). BUVEC were treated with different concentrations of butyrate, and a high increase in ICAM-1 expression and polymorphonuclear neutrophils (PMN) adhesion was observed at 10 mM butyrate. We found that only HCA2 was expressed in BUVEC, and its activation did not induce ICAM-1 expression. Analysis of intracellular pathways revealed that butyrate increased the phosphorylation of ERK1/2, and its inhibition reduced butyrate-induced ICAM-1 expression. We also demonstrated that BUVEC mainly express monocarboxylate transporter 1 (MCT1) and that its inhibition reduces butyrate-induced ICAM-1 expression. Finally, we demonstrated that butyrate acts as an HDAC inhibitor and MCT1 is essential for this response. Overall, these findings provide a possible mechanism for the effects of butyrate on bovine endothelial cells, which could explain the exacerbated inflammatory response during nutritional imbalance in cattle.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"170 ","pages":"Article 105439"},"PeriodicalIF":2.4,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144820879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Respiratory nematodiasis (Nematoda, Metastrongyloidea) is associated with marked type 2 inflammation in a marine mammal 在海洋哺乳动物中,呼吸线虫病与明显的2型炎症有关。
IF 2.4 3区 农林科学
Developmental and comparative immunology Pub Date : 2025-08-06 DOI: 10.1016/j.dci.2025.105437
Winson King Wai Tsang , Katherine Wu , Jared R Fischbach , Serena Zhang , Aranza Gomez-Camus , Margaret E. Martinez , Pádraig Duignan , Mauricio Seguel
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