Developmental and comparative immunology最新文献

{"title":"A novel lectin with a distinct Gal_Lectin and CUB domain mediates haemocyte phagocytosis in oyster Crassostrea gigas","authors":"Wenwen Yang ,&nbsp;Jiejie Sun ,&nbsp;Jinyuan Leng ,&nbsp;Yinan Li ,&nbsp;Qiuyan Guo ,&nbsp;Lingling Wang ,&nbsp;Linsheng Song","doi":"10.1016/j.dci.2024.105222","DOIUrl":"10.1016/j.dci.2024.105222","url":null,"abstract":"<div><p>Invertebrate lectins exhibit structural diversity and play crucial roles in the innate immune responses by recognizing and eliminating pathogens. In the present study, a novel lectin containing a Gal_Lectin, a CUB and a transmembrane domain was identified from the Pacific oyster <em>Crassostrea gigas</em> (defined as <em>Cg</em>Gal-CUB). <em>Cg</em>Gal-CUB mRNA was detectable in all the examined tissues with the highest expression in adductor muscle (11.00-fold of that in haemocytes, <em>p</em> &lt; 0.05). The expression level of <em>Cg</em>Gal-CUB mRNA in haemocytes was significantly up-regulated at 3, 24, 48 and 72 h (8.37-fold, 12.13-fold, 4.28-fold and 10.14-fold of that in the control group, respectively) after <em>Vibrio splendidus</em> stimulation. The recombinant <em>Cg</em>Gal-CUB (r<em>Cg</em>Gal-CUB) displayed binding capability to Mannan (MAN), peptidoglycan (PGN), D-(+)-Galactose and L-Rhamnose monohydrate, as well as Gram-negative bacteria (<em>Escherichia coli</em>, <em>V. splendidus</em> and <em>Vibrio anguillarum</em>), Gram-positive bacteria (<em>Micrococcus luteus</em>, <em>Staphylococcus aureus</em>, and <em>Bacillus sybtilis</em>) and fungus (<em>Pichia pastoris</em>). r<em>Cg</em>Gal-CUB was also able to agglutinate <em>V. splendidus</em>, and inhibit <em>V. splendidus</em> growth. Furthermore, r<em>Cg</em>Gal-CUB exhibited the activities of enhancing the haemocyte phagocytosis towards <em>V. splendidus</em>, and the phagocytosis rate of haemocytes was descended in blockage assay with <em>C</em>gGal-CUB antibody. These results suggested that <em>Cg</em>Gal-CUB served as a pattern recognition receptor to bind various PAMPs and bacteria, and enhanced the haemocyte phagocytosis towards <em>V. splendidus</em>.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141516727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Back to the future: Forgotten protocols for optimizing the isolation of arthropod haemocytes","authors":"","doi":"10.1016/j.dci.2024.105223","DOIUrl":"10.1016/j.dci.2024.105223","url":null,"abstract":"<div><p>Consideration is given to previous and more recent protocols for harvesting arthropod haemocytes from <em>Galleria</em>, <em>Drosophila</em>, mosquitoes, <em>Limulus</em> and crustaceans. The optimal harvesting of these cells is essential for meaningful studies of invertebrate immunity <em>in vitro</em>. The results of such experiments, however, have often been flawed due to a lack of understanding of the fragile nature of arthropod haemocytes on exposure to bacterial lipopolysaccharides, resulting in the aggregation and loss of cell types during haemolymph clotting. This article emphasizes that although there are similarities between mammalian neutrophils and arthropod haemocytes, the protocols required for the successful harvesting of these cells vary significantly. The various stages for the successful harvesting of arthropod haemocytes are described in detail and should provide invaluable advice to those requiring both high cell viability and recovery of the different cell types for subsequent experimentation.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0145305X24000958/pdfft?md5=c0c85743082f8be3ec6d67e843aef089&pid=1-s2.0-S0145305X24000958-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141497416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and functional characterization of ILYS-5, a major invertebrate lysozyme of Caenorhabditis elegans","authors":"Henry Berndt ,&nbsp;Silja Fuchs,&nbsp;Ina Kraus-Stojanowic,&nbsp;Barbara Pees ,&nbsp;Christoph Gelhaus,&nbsp;Matthias Leippe","doi":"10.1016/j.dci.2024.105220","DOIUrl":"10.1016/j.dci.2024.105220","url":null,"abstract":"<div><p>To overcome bacterial invasion and infection, animals have evolved various antimicrobial effectors such as antimicrobial peptides and lysozymes. Although <em>C. elegans</em> is exposed to a variety of microbes due to its bacterivorous lifestyle, previous work on the components of its immune system mainly based on the description of transcriptional changes during bacterial challenges. Very few effector components of its immune system have been characterized so far. To investigate the role of lysozymes in terms of antibacterial defense and digestion, we studied a member of the widely neglected family of <em>C. elegans</em> invertebrate lysozymes (ILYS). We focused on the so far virtually undescribed ILYS-5, which we purified from protein extracts of <em>C. elegans</em> tracing its peptidoglycan-degrading activity and localized the tissue expression of the gene <em>in vivo</em> using a translational reporter construct. We recombinantly synthesized ILYS-5 and determined the physicochemical activity optimum and the antibacterial spectrum of a lysozyme from <em>C. elegans</em> for the first time. With an activity optimum at low ionic strength (≤100 mM) and at acidic pH (≤ pH 4.0), ILYS-5 is likely to be involved in killing and digestion of bacteria within acidified phagolysosomes and acidic regions of the gut, presumably secreted by lysosome-like vesicles. This notion is supported by potent activity against various live Gram-positive and Gram-negative bacteria. Notably, members of the natural associated microbiome of <em>C. elegans</em> are substantially less susceptible to ILYS-5. Ablation of the <em>ilys-5</em> gene resulted in reduction of lifespan and fertility when cultured on the standard food bacterium <em>Escherichia coli</em> OP50, whereas exposure of the <em>ilys-5</em> knock-out mutant to the host-associated bacterium <em>Pseudomonas lurida</em> MYb11 did not have a clear effect. These findings indicate a role of ILYS-5 in immunity and nutrition and a co-evolved adaptation of host and bacteria to the mutualistic nature of their interaction.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0145305X24000922/pdfft?md5=fa72dc6cfe145464d66192ba0866770d&pid=1-s2.0-S0145305X24000922-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141455858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular matrix proteins Pericardin and Lonely heart mediate periostial hemocyte aggregation in the mosquito Anopheles gambiae","authors":"Cole J. Meier ,&nbsp;Shabbir Ahmed ,&nbsp;Jordyn S. Barr,&nbsp;Tania Y. Estévez-Lao,&nbsp;Julián F. Hillyer","doi":"10.1016/j.dci.2024.105219","DOIUrl":"10.1016/j.dci.2024.105219","url":null,"abstract":"<div><p>An infection induces the migration of immune cells called hemocytes to the insect heart, where they aggregate around heart valves called ostia and phagocytose pathogens in areas of high hemolymph flow. Here, we investigated whether the cardiac extracellular matrix proteins, Pericardin (Prc) and Lonely heart (Loh), regulate the infection-induced aggregation of periostial hemocytes in the mosquito, <em>An. gambiae</em>. We discovered that RNAi-based post-transcriptional silencing of <em>Prc</em> or <em>Loh</em> did not affect the resident population of periostial hemocytes in uninfected mosquitoes, but that knocking down these genes decreases the infection-induced migration of hemocytes to the heart. Knocking down <em>Prc</em> or <em>Loh</em> did not affect the proportional distribution of periostial hemocytes along the periostial regions. Moreover, knocking down <em>Prc</em> or <em>Loh</em> did not affect the number of sessile hemocytes outside the periostial regions, suggesting that the role of these proteins is cardiac-specific. Finally, knocking down <em>Prc</em> or <em>Loh</em> did not affect the amount of melanin at the periostial regions, or the intensity of an infection at 24 h after challenge. Overall, we demonstrate that Prc and Loh are positive regulators of the infection-induced migration of hemocytes to the heart of mosquitoes.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0145305X24000910/pdfft?md5=078d56ae84d8de93202a85601bc4330e&pid=1-s2.0-S0145305X24000910-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141455857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evidence for immune priming specificity and cross-protection against sympatric and allopatric Vibrio splendidus strains in the oyster Magalana (Crassostrea) gigas","authors":"Noushin Arfathery ,&nbsp;Charlotte Rafaluk ,&nbsp;Jens Rolff ,&nbsp;K. Mathias Wegner","doi":"10.1016/j.dci.2024.105221","DOIUrl":"10.1016/j.dci.2024.105221","url":null,"abstract":"<div><p>Infections with pathogenic <em>Vibrio</em> strains are associated with high summer mortalities of Pacific oysters <em>Magalana (Crassostrea) gigas</em>, affecting production worldwide. This raises the question of how <em>M. gigas</em> cultures can be protected against deadly <em>Vibro</em> infection. There is increasing experimental evidence of immune priming in invertebrates, where previous exposure to a low pathogen load boosts the immune response upon secondary exposure. Priming responses, however, appear to vary in their specificity across host and parasite taxa. To test priming specificity in the <em>Vibrio</em> – <em>M. gigas</em> system, we used two closely related <em>Vibrio splendidus</em> strains with differing degrees of virulence towards <em>M. gigas</em>. These <em>V. splendidus</em> strains were either isolated in the same location as the oysters (sympatric, opening up the potential for co-evolution) or in a different location (allopatric). We extracted cell-free haemolymph plasma from infected and control oysters to test the influence of humoral immune effectors on bacterial growth <em>in vitro</em>. While addition of haemolypmph plasma in general promoted growth of both strains, priming by an exposure to a sublethal dose of bacterial cells lead to inhibitory effects against a subsequent challenge with a potentially lethal dose <em>in vitro</em>. Inhibitory effects and immune priming was strongest when oysters had been primed with the sympatric <em>Vibrio</em> strain, but inhibitory effects were seen both when challenged with the sympatric as well as against allopatric V. splendidus, suggesting some degree of cross protection. The stronger immune priming against the sympatric strain suggests that priming could be more efficient against matching local strains potentially adding a component of local adaptation or co-evolution to immune priming in oysters. These <em>in vitro</em> results, however, were not reflected in the <em>in vivo</em> infection data, where we saw increased bacterial loads following an initial challenge. This discrepancy might suggests that that it is the humoral part of the oyster immune system that produces the priming effects seen in our <em>in vitro</em> experiments.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141455856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolutionarily conserved Otub1 suppresses antiviral immune response by promoting Irf3 proteasomal degradation in miiuy croaker, Miichthys miiuy","authors":"Yanli Su ,&nbsp;Tianjun Xu ,&nbsp;Yuena Sun","doi":"10.1016/j.dci.2024.105218","DOIUrl":"10.1016/j.dci.2024.105218","url":null,"abstract":"<div><p>Increasing evidence has been shown that OTUB1, a member of OTU deubiquitinases, is of importance in regulating the immune system. However, its molecular identification and functional characterization in teleosts are still rarely known. In this work, we cloned the <em>otub1</em> of miiuy croaker (<em>Miichthys miiuy</em>), analyzed its sequence, structure, and evolution at genetic and protein levels, and determined its function in the antiviral immune response. The complete open reading frame (ORF) of miiuy croaker <em>otub1</em> is 843 bp in length, encoding 280 amino acids. Miiuy croaker Otub1 has an OTU domain at the carboxyl terminus, which is a common functional domain that exists in OTU deubiquitinases. Molecular characteristics and evolution analysis results indicated that miiuy croaker Otub1, especially its functional domain, is highly conserved during evolution. The luciferase reporter assays showed that miiuy croaker Otub1 could significantly inhibit the poly(I:C) and Irf3-induced IFN1 and IFN-stimulated response element (ISRE) activation. Further experiments showed that miiuy croaker Otub1 decreases Irf3 protein abundance by promoting its proteasomal degradation. These data suggest that the evolutionarily conserved Otub1 acts as a suppressor in controlling antiviral immune response by promoting Irf3 proteasomal degradation in miiuy croaker.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141445827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Norepinephrine regulates TNF expression via the A1AR-p38 MAPK-Relish pathway in granulocytes of oyster Crassostrea gigas","authors":"Zhaoqun Liu ,&nbsp;Weilin Wang ,&nbsp;Yanan Zong ,&nbsp;Meijia Li ,&nbsp;Yuqian Gao ,&nbsp;Xiaoyu Xin ,&nbsp;Ting Zhu ,&nbsp;Lingling Wang ,&nbsp;Linsheng Song","doi":"10.1016/j.dci.2024.105217","DOIUrl":"10.1016/j.dci.2024.105217","url":null,"abstract":"<div><p>Norepinephrine (NE) is involved in regulating cytokine expression and phagocytosis of immune cells in the innate immunity of vertebrates. In the present study, the modulation mechanism of NE on the biosynthesis of TNFs in oyster granulocytes was explored. The transcripts of <em>Cg</em>TNF-1, <em>Cg</em>TNF-2 and <em>Cg</em>TNF-3 were highly expressed in granulocytes, and they were significantly up-regulated after LPS stimulation, while down-regulated after NE treatment. The phagocytic rate and apoptosis index of oyster granulocytes were also triggered by LPS stimulation and suppressed by NE treatment. The mRNA expressions of <em>Cg</em>MAPK14 and <em>Cg</em>Relish were significantly induced after NE treatment, and the translocation of <em>Cg</em>Relish from cytoplasm to nucleus was observed. The concentration of intracellular Ca²⁺ in granulocytes was significantly up-regulated upon NE incubation, and this trend reverted after the treatment with DOX (specific antagonist for NE receptor, <em>Cg</em>A1AR-1). No obvious significance was observed in intracellular cAMP concentrations in the PBS, NE and NE + DOX groups. Once <em>Cg</em>A1AR-1 was blocked by DOX, the mRNA expressions of <em>Cg</em>MAPK14 and <em>Cg</em>Relish were significantly inhibited, and the translocation of <em>Cg</em>Relish from cytoplasm to nucleus was also dramatically suppressed, while the mRNA expression of <em>Cg</em>TNF-1 and the apoptosis index increased significantly to the same level with those in LPS group, respectively. These results collectively suggested that NE modulated TNF expression in oyster granulocyte through A1AR-p38 MAPK-Relish signaling pathway.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141431588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Divergent humoral responses between males and females against 24 kDa excretory-secretory protein of Haemonchus contortus and influence of ovine β-globin polymorphism","authors":"Cintia Hiromi Okino ,&nbsp;Simone Cristina Méo Niciura ,&nbsp;Alessandro Pelegrine Minho ,&nbsp;Sérgio Novita Esteves ,&nbsp;Gláucia Roberta Melito ,&nbsp;Hélio José Montassier ,&nbsp;Ana Carolina de Souza Chagas","doi":"10.1016/j.dci.2024.105216","DOIUrl":"10.1016/j.dci.2024.105216","url":null,"abstract":"<div><p>Lambs harboring the Hb-AA β-globin haplotype present improved cell-mediated responses and increased resistance against <em>Haemonchus contortus</em> infection. The aim of the present study was to compare the effect of sex and β-globin haplotypes on specific humoral responses and phenotypes of resistance during <em>H. contortus</em> infection in Morada Nova sheep. As expected, females displayed stronger resistance during the first and second experimental challenges. Differential systemic humoral immune responses were observed comparing sex groups, in which higher levels of specific antibodies targeting 24 kDa excretory-secretory (ES24) protein of <em>H. contortus</em> of IgG and IgM antibodies were respectively observed as predominant isotypes in males and females. The IgM levels were significantly correlated with phenotypes of resistance, evaluated by packed cell volume and fecal egg counts. To our knowledge this is the first study reporting divergent humoral responses profiles to <em>H. contortus</em> infection between male and female sheep. The impact of β-globin haplotypes was less pronounced in females compared to males. Notably, only males showed significant weight differences across haplotypes, with Hb-AA lambs being the heaviest. Additionally, Hb-AA males had significantly higher PCV (indicating better red blood cell health) and lower FEC (indicating lower parasite burden). These findings suggest a more pronounced effect of β-globin polymorphisms on <em>H. contortus</em> infection in males, potentially due to their generally weaker resistance compared to females. This study highlights the importance of sex and β-globin haplotypes in shaping immune responses to <em>H. contortus</em> infection. Specifically, IgM antibodies targeting the ES24 protein appear to play a crucial role in host-parasite interactions and may hold promise for therapeutic development.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141431587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host response to Aplysia Abyssovirus 1 in nervous system and gill","authors":"Nicholas S. Kron ,&nbsp;Lynne A. Fieber ,&nbsp;Lydia Baker ,&nbsp;Catherine Campbell ,&nbsp;Michael C. Schmale","doi":"10.1016/j.dci.2024.105211","DOIUrl":"10.1016/j.dci.2024.105211","url":null,"abstract":"<div><p>The California sea hare (<em>Aplysia californica)</em> is a model for age associated cognitive decline. Recent researched identified a novel nidovirus, Aplysia Abyssovirus 1, with broad tropism enriched in the Aplysia nervous system. This virus is ubiquitous in wild and maricultured, young and old animals without obvious pathology. Here we re-evaluated gene expression data from several previous studies to investigate differential expression in the nervous system and gill in response to virus and aging as well as the mutational spectrum observed in the viral sequences obtained from these datasets. Viral load and age were highly correlated, indicating persistent infection. Upregulated genes in response to virus were enriched for immune genes and signatures of ER and proteostatic stress, while downregulated genes were enriched for mitochondrial metabolism. Differential expression with respect to age suggested increased iron accumulation and decreased glycolysis, fatty acid metabolism, and proteasome function. Interaction of gene expression trends associated with viral infection and aging suggest that viral infection likely plays a role in aging in the Aplysia nervous system. Mutation analysis of viral RNA identified signatures suggesting ADAR and AID/APOBEC like deaminase act as part of Aplysia anti-viral defense.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141396535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subclinical peripheral inflammation has systemic effects impacting central nervous system proteome in budgerigars","authors":"Eleni Voukali ,&nbsp;Daniel Divín ,&nbsp;Mercedes Goméz Samblas ,&nbsp;Nithya Kuttiyarthu Veetil ,&nbsp;Tereza Krajzingrová ,&nbsp;Martin Těšický ,&nbsp;Tao Li ,&nbsp;Balraj Melepat ,&nbsp;Pavel Talacko ,&nbsp;Michal Vinkler","doi":"10.1016/j.dci.2024.105213","DOIUrl":"https://doi.org/10.1016/j.dci.2024.105213","url":null,"abstract":"<div><p>Regulation of neuroimmune interactions varies across avian species. Little is presently known about the interplay between periphery and central nervous system (CNS) in parrots, birds sensitive to neuroinflammation. Here we investigated the systemic and CNS responses to dextran sulphate sodium (DSS)- and lipopolysaccharide (LPS)-induced subclinical acute peripheral inflammation in budgerigar (<em>Melopsittacus undulatus</em>). Three experimental treatment groups differing in DSS and LPS stimulation were compared to controls. Individuals treated with DSS showed significant histological intestinal damage. Through quantitative proteomics we described changes in plasma (PL) and cerebrospinal fluid (CSF) composition. In total, we identified 180 proteins in PL and 978 proteins in CSF, with moderate co-structure between the proteomes. Between treatments we detected differences in immune, coagulation and metabolic pathways. Proteomic variation was associated with the levels of pro-inflammatory cytokine mRNA expression in intestine and brain. Our findings shed light on systemic impacts of peripheral low-grade inflammation in birds.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141328324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}