传染性肌坏死病毒（IMNV）诱导凡纳滨对虾rnai相关基因表达上调。

IF 2.7 3区农林科学 Q1 FISHERIES

Developmental and comparative immunology Pub Date : 2025-01-01 DOI:10.1016/j.dci.2024.105296

Rubens Galdino Feijó , Jhonatas Teixeira Viana , Rodrigo Maggioni , Luis Fernando Marins

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引用次数: 0

摘要

传染性肌坏死病毒（IMNV）仍然给美国和亚洲的虾养殖业造成重大的经济和社会损失。本研究从RNAi机制研究了Sid-1、Dicer-2和Argonaute-2基因在自然感染了IMNV并注射含有1.02×105、1.02×104或1.02×103 IMNV拷贝·μL-1的接种剂的南美对虾（Penaeus vannamei）体内的转录模式。我们观察到，随着感染病毒浓度的增加，这些关键基因的转录水平受到影响。然而，在实验过程中，病毒载量并没有减少。我们认为Sid-1 mRNA表达的变化可以作为病毒复制的标记物，用于评价凡纳米养殖的卫生状况。

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Infectious myonecrosis virus (IMNV) induces upregulation of RNAi-related genes in white shrimp Penaeus vannamei

Infectious myonecrosis virus (IMNV) still causes significant economic and social losses in American and Asian shrimp farming. In this work, we investigated the transcription patterns of Sid-1, Dicer-2 and Argonaute-2 genes from the RNAi mechanism in Penaeus vannamei naturally infected with IMNV, and injected with inoculum containing 1.02 × 10⁵, 1.02 × 10⁴ or 1.02 × 10³ IMNV copies‧μL⁻¹. We observed that infection with increasing IMNV concentrations affected the transcription levels of these key genes. However, the viral load did not decrease during the experiment. We suggest that changes in Sid-1 mRNA expression could be used as marker of viral replication for evaluating sanitary status in P. vannamei farming.

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来源期刊

Developmental and comparative immunology 医学-动物学

CiteScore

6.20

自引率

6.90%

发文量

206

审稿时长

49 days

期刊介绍： Developmental and Comparative Immunology (DCI) is an international journal that publishes articles describing original research in all areas of immunology, including comparative aspects of immunity and the evolution and development of the immune system. Manuscripts describing studies of immune systems in both vertebrates and invertebrates are welcome. All levels of immunological investigations are appropriate: organismal, cellular, biochemical and molecular genetics, extending to such fields as aging of the immune system, interaction between the immune and neuroendocrine system and intestinal immunity.