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[A new method for static intensity-modulated radiation therapy]. [静态调强放射治疗的新方法]。
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-12-01
Chao-min Chen, Lin-hong Zhou, Qing-wen Lü, Hao-yu Jin, Mu-tao Tang, Guang-jie Chen
{"title":"[A new method for static intensity-modulated radiation therapy].","authors":"Chao-min Chen,&nbsp;Lin-hong Zhou,&nbsp;Qing-wen Lü,&nbsp;Hao-yu Jin,&nbsp;Mu-tao Tang,&nbsp;Guang-jie Chen","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To develop a new method for static intensity-modulated radiation therapy (IMRT) possible for implementation in smaller hospitals by incorporating accurate location equipments and treatment planning system in the available equipments for general radiotherapy.</p><p><strong>Methods: </strong>Based on the techniques of conformal radiotherapy, a new split method for fabricating the three-dimensional physical compensator was developed to achieve IMRT.</p><p><strong>Results: </strong>Experiment with the new method showed that the fabricated compensator could achieve good match between the high dosage shape in the target area and the dimensions of the tumor, and allowed adjustment of the dosage distribution according to the therapeutic requirement.</p><p><strong>Conclusion: </strong>This simple and feasible method allows cost-effect application of IMRT in smaller hospitals.</p>","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25751445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Liposome-mediated human CD40 gene transfection and human umbilical vein endothelial ECV-304 cells]. [脂质体介导的人CD40基因转染与人脐静脉内皮细胞ECV-304]。
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-12-01
Wei-rong Wang, Rong Lin, Yu-cong Yang, Wei-jie Gan, Jun-tian Liu, She-min Lü
{"title":"[Liposome-mediated human CD40 gene transfection and human umbilical vein endothelial ECV-304 cells].","authors":"Wei-rong Wang,&nbsp;Rong Lin,&nbsp;Yu-cong Yang,&nbsp;Wei-jie Gan,&nbsp;Jun-tian Liu,&nbsp;She-min Lü","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To construct an eukaryotic expression vector containing human CD40 gene for its efficient, continuous and stable expression in human umbilical vein endothelial ECV-304 cells.</p><p><strong>Methods: </strong>The recombinant plasmid pUCD40 was digested with endonucleases to obtain human CD40 gene fragment, which was cloned into pCDNA3.1 vector to construct recombinant eukaryotic expression vector pCDNA3.1(+)/CD40. The recombinant vector was identified by enzyme digestion before introduced into ECV-304 cells via liposome, with the positive cell clones selected with G418. The stable transfection and expression of CD40 in ECV-304 cells were identified by reverse transcription (RT)-PCR, Western blotting and flow cytometry, respectively.</p><p><strong>Results: </strong>Enzyme digestion analysis showed that target gene had been cloned into the recombinant vector. The transfected ECV-304 cells successfully expressed human CD40 as determined by RT-PCR and Western-blotting, and 95% of the cells were CD40-positive as shown by flow cytometry.</p><p><strong>Conclusion: </strong>The recombinant eukaryotic expression vector pCDNA3.1(+)/CD40 has been successfully constructed, which is capable of stable transfection and expression of CD40 in ECV-304 cells to facilitate further investigation of the roles of CD40 molecule in antiatherosclerotic drug development.</p>","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25751520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[bcl-2 expression in small cell lung cancer: a mechanism for apoptosis antagonism and immune evasion]. [bcl-2在小细胞肺癌中的表达:凋亡拮抗和免疫逃避的机制]。
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-12-01
Jun-meng Zheng, De-kun Wang, Hai-ming Jiang
{"title":"[bcl-2 expression in small cell lung cancer: a mechanism for apoptosis antagonism and immune evasion].","authors":"Jun-meng Zheng,&nbsp;De-kun Wang,&nbsp;Hai-ming Jiang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To observe bcl-2 expression and its correlation with apoptosis antagonism and immune evasion of small cell lung cancer cell line and explore the application of bcl-2 antisense thio-oligonucleotide (bcl-2 SON) in gene therapy for lung cancer.</p><p><strong>Methods: </strong>Western blotting was performed to detect bcl-2 expression in NCI-H69 cell line expressing Bcl-2 protein treated with bcl-2 SON. The treated NCI-H69 cells, along with the cells without bcl-2 SON treatment and NCI-H82 cells that did not express Bcl-2 protein, were respectively co-cultured with tumor-infiltrating lymphocytes (TILs) isolated from fresh tumor samples, and the apoptosis of the lung cancer cells was assessed by JAM assay.</p><p><strong>Results: </strong>Western blotting revealed obvious inhibition of bcl-2 expression in NCI-H69 cells in response to bcl-2 SON treatment. JAM assay showed that the apoptosis of bcl-2 SON-treated H69 cells and NCI-H82 cells increased with the elevation of TIL ratio in the co-culture, while such changes were not observed in NCI-H69 cells without bcl-2 SON treatment.</p><p><strong>Conclusion: </strong>Small cell lung cancer cells expressing bcl-2 may antagonize the antitumor immune attack in the host, and bcl-2 SON may provide an effective alternative in gene therapy for small cell lung cancer.</p>","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25756302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Apoptosis induced by curcumin and its effect on c-myc and caspase-3 expressions in human melanoma A375 cell line]. 姜黄素诱导人黑色素瘤A375细胞株凋亡及其对c-myc和caspase-3表达的影响
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-12-01
Shi Qiu, Sheng-shun Tan, Jiang-an Zhang, An Liu, Jing-yi Yuan, Guo-zhou Rao, Wen-yong Wang
{"title":"[Apoptosis induced by curcumin and its effect on c-myc and caspase-3 expressions in human melanoma A375 cell line].","authors":"Shi Qiu,&nbsp;Sheng-shun Tan,&nbsp;Jiang-an Zhang,&nbsp;An Liu,&nbsp;Jing-yi Yuan,&nbsp;Guo-zhou Rao,&nbsp;Wen-yong Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect of curcumin on cell apoptosis and c-myc and caspase-3 expressions in human melanoma A375 cell line.</p><p><strong>Methods: </strong>A375 cells were exposed to curcumin treatment and growth inhibition of the cells was examined by MTT assay. Annexin V/propidium iodide double staining and DNA fragmentation analysis were employed for assay of the cell apoptosis and morphological changes of the cells were observed with inverted microscopy and transmission electron microscopy, respectively. In situ hybridization and SABC immunohistochemistry were performed for detection of the expressions of c-Myc and caspase-3 in the A375 cells.</p><p><strong>Results: </strong>Curcumin inhibited the growth of A375 cells in both time- and concentration-dependent manners. After treatment with 30 micromol/L curcumin for 48 h, apoptotic morphological changes were observed in the cells and an oligonucleosomal DNA ladder was clearly visualized in DNA fragmentation analysis. The apoptotic rates of the cells treated with curcumin at the concentration above 20 micromol/L were significantly higher than that of the control cells. c-myc expression level was decreased whereas caspase-3 expression increased with the increase in curcumin concentrations.</p><p><strong>Conclusion: </strong>Curcumin can inhibit the proliferation and induce apoptosis of A375 cells in vitro, and the genes encoding c-myc and caspase-3 may play a role in the process.</p>","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25756376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Report of 1 case of rare giant metastatic intra-ocular tumor]. 【罕见巨大转移性眼内肿瘤1例报告】。
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-12-01
Qiu-hong Liu, Yu-jian Zhan, Yang-gui Yu
{"title":"[Report of 1 case of rare giant metastatic intra-ocular tumor].","authors":"Qiu-hong Liu,&nbsp;Yu-jian Zhan,&nbsp;Yang-gui Yu","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25788291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Expression of HLA-G protein in trophoblast cells]. 滋养细胞中HLA-G蛋白的表达。
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-12-01
Yan-qiu Wang, Shi-ling Chen, Fu-qi Xing
{"title":"[Expression of HLA-G protein in trophoblast cells].","authors":"Yan-qiu Wang,&nbsp;Shi-ling Chen,&nbsp;Fu-qi Xing","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the expression of human leucocyte antigen protein G (HLA-G) in different trophoblast cells and different stages of pregnancy.</p><p><strong>Methods: </strong>The expression of HLA-G protein in normal placenta and trophoblasts of different trimesters was detected using immunohistochemical method (SP).</p><p><strong>Results: </strong>HLA-G protein expression exhibited spatio-temporal changes, which located in the extravillous trophoblast (EVT) and was higher in the placenta of the first and second trimesters while lower in the third trimester (P<0.01).</p><p><strong>Conclusion: </strong>HLA-G protein expression in different stages of pregnancy and different trophoblasts may be related to the controlled invasion of the trophoblast.</p>","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25751443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Selection of femoral prosthesis in total hip replacement for ankylosing spondylitis. 强直性脊柱炎全髋关节置换术中股骨假体的选择。
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-12-01
Pei Yang, Chun-sheng Wang, Kun-zheng Wang, Zhi-Bin Shi, Fu-liang Gong
{"title":"Selection of femoral prosthesis in total hip replacement for ankylosing spondylitis.","authors":"Pei Yang,&nbsp;Chun-sheng Wang,&nbsp;Kun-zheng Wang,&nbsp;Zhi-Bin Shi,&nbsp;Fu-liang Gong","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the implication of morphological changes of the proximal femur in femoral prosthesis selection in total hip replacement for treatment of ankylosing spondylitis (AS).</p><p><strong>Methods: </strong>Twenty-four patients (36 hips) admitted for cementless total hip replacement (THR) because of AS were enrolled in this study along with the control group of 30 patients (45 hips) without AS. Measurement of the Singh index, canal flare index, morphological index of the cortex, cortical index and stem-canal fit was performed in the two groups on the basis of roentgenograms.</p><p><strong>Results: </strong>The AS patients had more serious osteoporosis in their proximal femur. There were significant differences between the two groups in Singh index, canal flare index, morphological index of the cortex and stem-canal fit (P<0.05), but not in the cortical index (P>0.05). The AS patients were found to have severe osteoporosis in the upper femur and massive spongy bone loss, so that the morphology of the canal was almost orthostatic in the upper femur because of the thinned femoral cortex.</p><p><strong>Conclusion: </strong>Cemented prosthesis is recommended for AS patients with severe osteoporosis, who should be treated with revised THR to ensure good fit of the prosthesis to the canal. Cementless prosthesis, however, can still achieve good therapeutic effect in AS patients without significant morphologic changes in the proximal femurs. Computer-aided design and manufacture of individualized prosthesis better suits the AS patients than the currently available prosthesis.</p>","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25751519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Clinical nature of tetrodotoxin intoxication and the measures for emergency rescue]. 河豚毒素中毒的临床特点及急救措施
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-12-01
Gui-zhen Liu, Jia-lin Che, Ming Xiao, Zuo-hua Fan
{"title":"[Clinical nature of tetrodotoxin intoxication and the measures for emergency rescue].","authors":"Gui-zhen Liu,&nbsp;Jia-lin Che,&nbsp;Ming Xiao,&nbsp;Zuo-hua Fan","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To define the clinical nature of tetrodotoxin intoxication and explore the measures for its emergency rescue.</p><p><strong>Methods: </strong>The emergency rescue measures and outcomes of 33 cases tetrodotoxin intoxication were reviewed.</p><p><strong>Results: </strong>No obvious effects were observed in these patients after administration of atropine or application of respiratory stimulant, but implementation of mechanical ventilation and comprehensive life support measures resulted in successful rescue of mild to moderate cases within 10 to 14 h, and recovery of spontaneous respiration was achieved in critical cases in 19-24 h.</p><p><strong>Conclusion: </strong>Tetrodotoxin selectively blocks the neuromuscular electrical activity, producing the effect of nondepolarizing curine and leading to failure of the respiratory drive without compromising the respiratory center. Atropine, a M-cholinergic receptor blocker, or central stimulants may fail to produce obvious effects, but comprehensive treatments including maintenance of effective ventilation can bring satisfactory effects.</p>","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25756298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Therapeutic effect of TAX combined with Herceptin or epirubicin against breast cancer positive for Her-2/neu]. [TAX联合赫赛汀或表柔比星治疗Her-2/neu阳性乳腺癌的疗效]。
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-12-01
Fei Cui, Rong-cheng Luo, Jin-zhang Chen, Bin Chen, Yu-xian Huang
{"title":"[Therapeutic effect of TAX combined with Herceptin or epirubicin against breast cancer positive for Her-2/neu].","authors":"Fei Cui,&nbsp;Rong-cheng Luo,&nbsp;Jin-zhang Chen,&nbsp;Bin Chen,&nbsp;Yu-xian Huang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To compare the therapeutic effects of biochemotherapy regimen with Herceptin plus taxol (TAX) and the chemotherapy regimen with epirubicin plus TAX against Her-2/neu-positive breast cancer and observe the changes in serum tumor markers in patients receiving biochemotherapy.</p><p><strong>Methods: </strong>Seventy-three patients with advanced breast cancer positive for Her-2/neu as revealed by immunohistochemistry were divided into the study group (n=32) to receive treatment with the regimen of Herceptin plus TAX and control group (n=41) with the regimen of epirubicin(EPI) plus TAX. The therapeutic effects of the regimens were observed and in the study group, the relationship of the therapeutic effect with Her-2/neu positivity and changes in serum tumor markers were examined.</p><p><strong>Results: </strong>The objective response rate and clinical benefit response rate were obviously higher in the study group than in the control group. In the study group, the clinical response rate of patients with positive immunostaining for Her-2/neu of grades 1+, 2+ and 3+ were 0%, 44.4% and 63.6%, respectively, as compared with those in the control group of 8.3%, 36.4%, and 38.9%, respectively, and the treatment resulted in lowered levels of serum tumor markers without significant changes in CA153, tps and CEA (P<0.05) after treatment, but CA125 showed no significant difference (P>0.05).</p><p><strong>Conclusions: </strong>In patients with advanced breast cancer with positive immunostaining for Her-2/neu of grade 3+, the regimen of Herceptin plus TAX can be more effective than the chemotherapeutic regimen of EPI plus TAX. Patients with Her-2/neu (+++) benefit more from the treatment than those with Her-2/neu (++). Serum CEA, CA153 and TPS levels also possess some value in evaluating the therapeutic effects of the regimens.</p>","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25756301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Surgical treatment of anterior circulation aneurysm via pterion keyhole approach]. [翼点锁孔入路治疗前循环动脉瘤]。
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Pub Date : 2005-12-01
Wen-feng Feng, Song-tao Qi, Sheng-ping Huang, Li-jin Huang
{"title":"[Surgical treatment of anterior circulation aneurysm via pterion keyhole approach].","authors":"Wen-feng Feng,&nbsp;Song-tao Qi,&nbsp;Sheng-ping Huang,&nbsp;Li-jin Huang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the feasibility of pterion keyhole approach with minimal invasion for treatment of the anterior circulation aneurysm.</p><p><strong>Method: </strong>Aneurysm clipping through the pterion keyhole approach was performed in patients with anterior circulation aneurysms, including 9 with posterior communication artery aneurysms, 3 with middle cerebral artery aneurysms and 6 with anterior communication artery aneurysms, who were in stages I to III according to Hunt-Hess scale.</p><p><strong>Result: </strong>All the aneurysms were clipped successfully. One patient with a left posterior communication artery aneurysm developed transient sensory aphasia and motor aphasia after surgery due to intraoperative aneurysm rupture. No facial paralysis occurred due to damage of the facial nerve.</p><p><strong>Conclusion: </strong>The pterion keyhole approach is a very useful surgical approach for treatment of anterior circulation aneurysms on the basis of cautious determination of indications and careful operation planning.</p>","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25756305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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