[bcl-2在小细胞肺癌中的表达:凋亡拮抗和免疫逃避的机制]。

Jun-meng Zheng, De-kun Wang, Hai-ming Jiang
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引用次数: 0

摘要

目的:观察小细胞肺癌细胞系bcl-2的表达及其与凋亡拮抗和免疫逃避的关系,探讨bcl-2反义硫代寡核苷酸(bcl-2 SON)在肺癌基因治疗中的应用。方法:采用Western blotting检测bcl-2在表达bcl-2蛋白的NCI-H69细胞株中表达情况。将经处理的NCI-H69细胞、未经bcl-2 SON处理的细胞和未表达bcl-2 SON的NCI-H82细胞分别与新鲜肿瘤样本中分离的肿瘤浸润淋巴细胞(til)共培养,采用JAM法检测肺癌细胞的凋亡情况。结果:Western blot结果显示,bcl-2 SON对NCI-H69细胞bcl-2表达有明显抑制作用。JAM实验显示,在共培养中,bcl-2 SON处理的H69细胞和NCI-H82细胞的凋亡随着TIL比例的升高而增加,而未处理bcl-2 SON的NCI-H69细胞则没有这种变化。结论:表达bcl-2的小细胞肺癌细胞可拮抗宿主体内的抗肿瘤免疫攻击,bcl-2 SON可能是小细胞肺癌基因治疗的有效替代方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[bcl-2 expression in small cell lung cancer: a mechanism for apoptosis antagonism and immune evasion].

Objective: To observe bcl-2 expression and its correlation with apoptosis antagonism and immune evasion of small cell lung cancer cell line and explore the application of bcl-2 antisense thio-oligonucleotide (bcl-2 SON) in gene therapy for lung cancer.

Methods: Western blotting was performed to detect bcl-2 expression in NCI-H69 cell line expressing Bcl-2 protein treated with bcl-2 SON. The treated NCI-H69 cells, along with the cells without bcl-2 SON treatment and NCI-H82 cells that did not express Bcl-2 protein, were respectively co-cultured with tumor-infiltrating lymphocytes (TILs) isolated from fresh tumor samples, and the apoptosis of the lung cancer cells was assessed by JAM assay.

Results: Western blotting revealed obvious inhibition of bcl-2 expression in NCI-H69 cells in response to bcl-2 SON treatment. JAM assay showed that the apoptosis of bcl-2 SON-treated H69 cells and NCI-H82 cells increased with the elevation of TIL ratio in the co-culture, while such changes were not observed in NCI-H69 cells without bcl-2 SON treatment.

Conclusion: Small cell lung cancer cells expressing bcl-2 may antagonize the antitumor immune attack in the host, and bcl-2 SON may provide an effective alternative in gene therapy for small cell lung cancer.

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