{"title":"Biocompatibility and pro-mineralization effects of premixed calcium silicate-based materials on human dental pulp stem cells: An in vitro and in vivo study.","authors":"Nyein Chan Ko, Sonoko Noda, Yamato Okada, Kento Tazawa, Nobuyuki Kawashima, Takashi Okiji","doi":"10.4012/dmj.2024-121","DOIUrl":"10.4012/dmj.2024-121","url":null,"abstract":"<p><p>Premixed calcium silicate-based materials have recently been developed and are recommended for a wide range of endodontic procedures, including vital pulp therapy. This study investigated the in vitro biocompatibility and pro-mineralization effect and in vivo reparative dentin formation of EndoSequence Root Repair Material, EndoSequence BCRRM, Bio-C Repair, and Well-pulp PT. Both fresh and set extracts had no detrimental effect on the growth of human dental pulp stem cells. The fresh extracts had a higher calcium concentration than the set extracts and induced considerably greater mineralized nodule formation. EndoSequence Root Repair Material had the longest setting time, whereas Bio-C Repair had the shortest. When these materials were applied to exposed rat molar pulps, mineralized tissue deposition was found at the exposure sites after 2 weeks. These results indicate that the premixed calcium silicate-based materials tested could have positive benefits for direct pulp capping procedures.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":" ","pages":"729-737"},"PeriodicalIF":1.9,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tobias Graf, Josef Schweiger, Janosch Goob, Michael Stimmelmayr, Isabel Lente, Oliver Schubert
{"title":"Dimensional reliability in CAD/CAM production of complete denture bases: A comparative study of milling and various 3D printing technologies.","authors":"Tobias Graf, Josef Schweiger, Janosch Goob, Michael Stimmelmayr, Isabel Lente, Oliver Schubert","doi":"10.4012/dmj.2023-215","DOIUrl":"10.4012/dmj.2023-215","url":null,"abstract":"<p><p>This study aims to assess the dimensional accuracy of complete denture bases fabricated from different CAD/CAM technologies and a conventional method, including milling (CNC), PolyJet (PJ), laser sintering (SLS), digital light processing (DLP), and injection molding (IM). It also examines the influence of the removal of technology-specific connectors or support structures when present. Denture base surfaces were digitized using a laboratory scanner, and virtual measurement points were calculated with tetrahedral reference geometries. Defined distances were measured in all spatial directions and compared to design data (p<0.05), revealing significant differences in sagittal (p=0.004), transversal (p<0.001), and vertical (p<0.001) dimensions. Connector removal had no significant impact for CNC but significantly affected DLP. All technologies yielded clinically acceptable results, with CNC milling demonstrating the best overall outcome.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":" ","pages":"629-636"},"PeriodicalIF":1.9,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141970844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oskar Bunz, Max Diekamp, Mozhgan Bizhang, Holger Testrich, Andree Piwowarczyk
{"title":"Surface roughness associated with bacterial adhesion on dental resin-based materials.","authors":"Oskar Bunz, Max Diekamp, Mozhgan Bizhang, Holger Testrich, Andree Piwowarczyk","doi":"10.4012/dmj.2023-234","DOIUrl":"10.4012/dmj.2023-234","url":null,"abstract":"<p><p>This study investigates the surface quality and bacterial adhesion properties of various dental materials, including indirect composites, veneering composites, direct composites, polyether ether ketone (PEEK), and two millable polymethyl methacrylate (PMMA). Material specimens were processed following manufacturer instructions, initially evaluated for surface roughness and Streptococcus sanguinis (S. sanguinis) adhesion. Subsequently, toothbrushing simulation was employed to simulate aging, and changes in material surfaces were assessed via roughness measurements and bacterial adhesion testing. Prior to simulated aging, direct and indirect composites exhibited the lowest roughness values. However, after the simulated toothbrushing, veneering composites displayed the highest roughness levels. Both PMMA materials demonstrated the highest S. sanguinis adhesion levels, both before and after artificial aging. Interestingly, the indirect composite material showed a reduction in bacterial adhesion following toothbrushing simulation. Surprisingly, this study did not reveal a clear correlation between roughness and bacterial adhesion.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":" ","pages":"621-628"},"PeriodicalIF":1.9,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hong Wang, Xinze Weng, Yan Chen, Shunjie Mao, Yuerong Gao, Qinglin Wu, Yanling Huang, Xin Guan, Zhiqiang Xu, Yingzhen Lai
{"title":"Biomimetic concentric microgrooved titanium surfaces influence bone marrow-derived mesenchymal stem cell osteogenic differentiation via H3K4 trimethylation epigenetic regulation.","authors":"Hong Wang, Xinze Weng, Yan Chen, Shunjie Mao, Yuerong Gao, Qinglin Wu, Yanling Huang, Xin Guan, Zhiqiang Xu, Yingzhen Lai","doi":"10.4012/dmj.2023-327","DOIUrl":"10.4012/dmj.2023-327","url":null,"abstract":"<p><p>Material surface micromorphology can modulate cellular behavior and promote osteogenic differentiation through cytoskeletal rearrangement. Bone reconstruction requires precise regulation of gene expression in cells, a process governed by epigenetic mechanisms such as histone modifications, DNA methylation, and chromatin remodeling. We constructed osteon-mimetic concentric microgrooved titanium surfaces with different groove sizes and cultured bone marrow-derived mesenchymal stem cells (BMSCs) on the material surfaces to study how they regulate cell biological behavior and osteogenic differentiation through epigenetics. We found that the cells arranged in concentric circles along the concentric structure in the experimental group, and the concentric microgrooved surface did not inhibit cell proliferation. The results of a series of osteogenic differentiation experiments showed that the concentric microgrooves facilitated calcium deposition and promoted osteogenic differentiation of the BMSCs. Concentric microgrooved titanium surfaces that were 30 μm wide and 10 μm deep promoted osteogenic differentiation of BMSC by increasing WDR5 expression via H3K4 trimethylation upregulation.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":" ","pages":"683-692"},"PeriodicalIF":1.9,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141970842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kanae Wada, Junichiro Wada, Mine Betul Uctasli, Sadullah Uctasli, Yasemin Yavuz, Tsutomu Iwamoto, Pekka K Vallittu, Sufyan Garoushi, Lippo Lassila
{"title":"Impact of restorative material on fracture behaviors of class II restoration in endodontically treated deciduous molars.","authors":"Kanae Wada, Junichiro Wada, Mine Betul Uctasli, Sadullah Uctasli, Yasemin Yavuz, Tsutomu Iwamoto, Pekka K Vallittu, Sufyan Garoushi, Lippo Lassila","doi":"10.4012/dmj.2024-067","DOIUrl":"10.4012/dmj.2024-067","url":null,"abstract":"<p><p>The purpose of this study was to investigate the fracture behavior of endodontically treated (ET) deciduous molar when directly restored with different restorative materials in Class II (MO) cavities in comparison with permanent teeth. MO cavities were prepared with 2.4-2.5 mm and 1.9-2.0 mm in buccolingual width, and mesiodistal width of each cavity walls, respectively, followed by direct restoration with different materials: resin-modified glass ionomer cement (RMGIC), composite resin (CR), and composite resin containing 25% short glass-fiber (SFRC). All specimens were subjected to mechanical loading tests at a speed of 1 mm/min and evaluated fracture resistance and fracture modes. A one-way ANOVA followed by a Tukey multiple comparisons analysis was used. Deciduous-SFRC (3,310.5±396.2 N) were significantly higher fracture resistance than permanent-RMGIC (1,633.8±346.8 N) (p<0.001), and permanent-CR (1,400.0±381.3 N) (p<0.001). For the direct restoration of MO cavity after endodontic treatment, SFRC demonstrated its promising performance in load-bearing capacity and failure mode, especially in ET deciduous molars.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":" ","pages":"738-745"},"PeriodicalIF":1.9,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142092518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Finite element analysis of fatigue life of commercially pure titanium clasps additively manufactured with different building orientations.","authors":"Kento Odaka, Mikiya Sugano, Taichi Kawamoto, Naoki Takano, Satoru Matsunaga","doi":"10.4012/dmj.2024-023","DOIUrl":"10.4012/dmj.2024-023","url":null,"abstract":"<p><p>The geometrical accuracy of additively manufactured pure titanium clasps depends on the building orientation. The aim of this study is to compare the geometrical accuracy and the fatigue lives predicted by finite element analysis (FEA) among three clasps manufactured with different building orientations. Besides, this paper proposed a calculation method of the moment of inertia of area and cross-sectional area along with the arm as the geometrical parameters. One of the clasps manufactured with a cylindrical chucking part for the fatigue test had almost the same geometrical parameters with the CAD design. Also, the authors' fatigue life prediction method using the CAD based FEA was verified through comparison with micro-CT image-based FEA. The other two clasps had larger geometrical parameters than the CAD design, resulting in longer fatigue lives. The results implied the importance of calculating the moment of inertia of the area in the design of the clasp arm.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":" ","pages":"656-666"},"PeriodicalIF":1.9,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141970845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Changes in the surface texture of pre-polymerized acrylic resin pediatric crowns following acidulated phosphate fluoride application.","authors":"Keiya Nanri, Hiroyasu Koizumi, Haruto Hiraba, Takashi Kikuiri, Takayuki Yoneyama","doi":"10.4012/dmj.2024-083","DOIUrl":"10.4012/dmj.2024-083","url":null,"abstract":"<p><p>This study aimed to investigate the effect of acidulated phosphate fluoride (APF) application on filler-free polymethyl methacrylate (PMMA)-based resin blocks for computer-aided design/computer-aided manufacturing (CAD-CAM), focusing on their use in pediatric crowns. Three types of PMMA-based blocks for CAD-CAM were evaluated, and a composite resin block for CAD-CAM was used as a control. Statistical analysis (p<0.05) of the data revealed that all PMMA-based blocks showed significantly higher gloss levels than the composite resin blocks. Two PMMA-based blocks also demonstrated significantly lower Ra and Sa values. SEM images showed no irregular changes in the surface properties of the PMMA-based blocks compared to those of the composite resin block. These results are significant in meeting the increasing demand for esthetic restorative treatments in pediatrics, where APF is commonly used for caries prevention. PMMA-based resin blocks for CAD-CAM are an effective alternative to prevent esthetic degradation from gloss reduction and plaque accumulation.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":" ","pages":"711-717"},"PeriodicalIF":1.9,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Karina Erda Saninggar, Fumika Abe, Ayana Nakano, Koichi Kato
{"title":"Collagen-binding bone morphogenetic protein-2 designed for use in bone tissue engineering.","authors":"Karina Erda Saninggar, Fumika Abe, Ayana Nakano, Koichi Kato","doi":"10.4012/dmj.2024-138","DOIUrl":"10.4012/dmj.2024-138","url":null,"abstract":"<p><p>Bone tissue engineering using biodegradable porous scaffolds is a promising approach for restoring oral and maxillofacial bone defects. Recently, attempts have been made to incorporate proteins such as growth factors to create bioactive scaffolds that can engage cells to promote tissue formation. Collagen-based scaffolds containing bone morphogenetic protein-2 (BMP2) have been studied for bone formation. However, controlling the initial burst of BMP2 remains difficult. Here we designed a functional chimeric protein composed of BMP2 and a collagen-binding domain (CBD), specifically the A3 domain of von Willebrand factor, to sustain BMP2 release from collagen-based scaffolds. Based on the results of computer-based structural prediction, we prepared a chimeric protein consisting of CBD and BMP2 in this order with a peptide tag for affinity purification. The chimeric protein had a collagen-binding capacity and enhanced osteogenic differentiation of human mesenchymal stem cells. These results are consistent with insights from in silico structural prediction.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":" ","pages":"718-728"},"PeriodicalIF":1.9,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hoda Mohamed Elnawawy, Muralithran Govindan Kutty, Noor Azlin Yahya, Noor Hayaty Abu Kasim, Paul Roy Cooper, Josette Camilleri, Hany Mohamed Aly Ahmed
{"title":"Chemical and physical properties of radiopaque Portland cement formulation with reduced particle size.","authors":"Hoda Mohamed Elnawawy, Muralithran Govindan Kutty, Noor Azlin Yahya, Noor Hayaty Abu Kasim, Paul Roy Cooper, Josette Camilleri, Hany Mohamed Aly Ahmed","doi":"10.4012/dmj.2024-015","DOIUrl":"10.4012/dmj.2024-015","url":null,"abstract":"<p><p>This study compared the chemical and physical properties of an experimental radiopaque white Portland cement (REPC) with reduced particle size to ProRoot white mineral trioxide aggregate (WMTA). The particle size distribution of experimental Portland cement (EPC) was examined, and then nano-zirconium oxide (nano-ZrO) was added to produce REPC. Chemical analysis, initial setting time, pH values, and push-out bond strength were evaluated. Results showed that REPC had smallest particle size (354.5±26.45 nm), while PC had the largest (1,309.67±60.54 nm) (p<0.05). Differences in chemical composition were observed. REPC exhibited shorter setting time (32.7±0.58 min) compared to WMTA (131.67±2.89 min) and PC (163.33±2.89 min) (p<0.05). All groups showed alkaline pH (p<0.05). REPC demonstrated the highest push-out bond strength (22.24±4.33 MPa) compared with WMTA (15.53±3.26 MPa) and PC (16.8±5.43 MPa) (p<0.05). This cost-effective PC formulation reduced the setting time and increased the push-out bond strength while maintaining the alkaline properties of the original cements.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":" ","pages":"672-682"},"PeriodicalIF":1.9,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142281771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of polishing methods for two types of monolithic all-ceramic crowns after occlusal adjustments: Polishing paste versus glazed porcelain.","authors":"Syuntaro Nomoto, Mizuho Hirano, Yu Tsuyuki, Takanori Sakai, Mamoru Yotsuya, Hideshi Sekine","doi":"10.4012/dmj.2024-058","DOIUrl":"10.4012/dmj.2024-058","url":null,"abstract":"<p><p>This study compared the effects of two surface preparation methods on two types of zirconia. Immediately prior to the placement of a monolithic zirconia crown, its morphology may be modified using a rotary cutting instrument for occlusal adjustments. The crown surface is scratched during the grinding process and, thus, requires polishing. Simplified zirconia crowns of 3Y and 5Y were fabricated and used as specimens. The surface roughness and gloss of the occlusal surfaces of specimens were measured and compared when a polishing compound was used after polishing points and when a silica-based coating was sintered. No significant differences were observed in surface roughness between 3Y and 5Y zirconia. The use of polishing compounds was effective because polishing points alone only resulted in a level of surface roughness that may cause wear on antagonist teeth. Although the silica-based coating improved surface properties, the polishing compound more effectively improved surface roughness.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":" ","pages":"667-671"},"PeriodicalIF":1.9,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141970843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}