Nouf Ali Alblooshi, Jogikalmat Krithikadatta, Cynthia Yiu, Mohammed Nadeem Bijle
{"title":"Fluoride release potential of arginine-incorporated fluoride varnishes.","authors":"Nouf Ali Alblooshi, Jogikalmat Krithikadatta, Cynthia Yiu, Mohammed Nadeem Bijle","doi":"10.4012/dmj.2023-238","DOIUrl":"10.4012/dmj.2023-238","url":null,"abstract":"<p><p>The study aimed to examine the fluoride (F) release potential of arginine (Arg)-incorporated F varnishes. Four commercially available F varnishes were included in the study: Duraphat<sup>®</sup> (5% NaF), Flúor Protector<sup>®</sup> (0.9% SiH<sub>2</sub>F<sub>2</sub>), Fluor Protector S<sup>®</sup> (NH<sub>4</sub>F), and Fluorimax™ (2.5% NaF). L-arginine (2% w/v.) was incorporated in these varnishes to estimate F release at 1 h, 4 h, 6 h, 24 h, 3 days, and 7 days using an F-ion selective electrode. The media pH of eluded varnishes was estimated and primary inorganic F extraction was performed. The main effects pH, F release, and computed integrated mean/cumulative F release for experimental groups were significantly higher than the controls (p<0.01). The primary extracted F concentrations for the Arg-containing groups were significantly lower than the control groups (p<0.001) demonstrating a chemical interplay with Arg incorporation. To conclude, irrespective of the inorganic F content, incorporating Arg in F-containing varnishes increases their F release potential.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139484732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Agata Szczesio-Wlodarczyk, Sufyan Garoushi, Pekka Vallittu, Kinga Bociong, Lippo Lassila
{"title":"Polymerization shrinkage stress of contemporary dental composites: Comparison of two measurement methods.","authors":"Agata Szczesio-Wlodarczyk, Sufyan Garoushi, Pekka Vallittu, Kinga Bociong, Lippo Lassila","doi":"10.4012/dmj.2023-192","DOIUrl":"10.4012/dmj.2023-192","url":null,"abstract":"<p><p>The aim of this study was to compare two testing methodologies employed for assessing the polymerization shrinkage stress of dental resin composites. Ten commercial resin composites were investigated (EverX Posterior & Flow; G-ænial Anterior, Posterior, A'CHORD & Universal Injectable; Filtek One Bulk Fill & Universal Restorative; SDR flow+ and Aura Bulk Fill). Photoelastic and contraction forces measurement methods were performed. The slope of the linear trendline and C-factor of specimens were calculated. The shrinkage stress values (range between: 6.4-13.4 MPa) obtained by the photoelastic method were higher for all resin composites than the values obtained by contraction forces measurements (range between 1.2-4.8 MPa). However, there was a strong linear correlation between these methods (r=0.8). The use of both investigated methods revealed important information about the shrinkage behavior of the restorative resin composites.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Toshiki Takamizawa, Ryota Aoki, Kana Hayashi, Mone Shoji, Yuta Kasahara, Wayne W Barkmeier, Mark A Latta, Atsushi Kamimoto, Masashi Miyazaki
{"title":"Scanning electron microscopy observation of dentin bond interfaces in different types of resin luting cements.","authors":"Toshiki Takamizawa, Ryota Aoki, Kana Hayashi, Mone Shoji, Yuta Kasahara, Wayne W Barkmeier, Mark A Latta, Atsushi Kamimoto, Masashi Miyazaki","doi":"10.4012/dmj.2023-219","DOIUrl":"10.4012/dmj.2023-219","url":null,"abstract":"<p><p>This study aimed to observe and compare the resin luting cement (RLC)/dentin interfaces of different types of RLC systems with scanning electron microscopy (SEM). This study evaluated six self-adhesive resin luting cement systems (SARLCs), four systems combining a SARLC with a tooth primer (SARLCPs), and six conventional resin luting cement systems (CVRLCs). Cured resin composite rods were bonded to bovine dentin using RLCs in different etching modes. The morphological features at the interfaces were observed using SEM. Although all RLCs in etch-&-rinse (ER) mode showed a clear hybrid layer, most SARLCs had a thinner and more porous hybrid layer than the SARLCPs and CVRLCs. All SARLCPs with primer and CVRLCs showed a thin high-density layer below the primer layer in both etching modes. CVRLCs and SARLCPs with primer treatment systems may be more versatile and reliable when compared to simplified RLC systems.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139511871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mario Alberto Alarcón-Sánchez, Natividad Castro-Alarcón, Daniel Sandoval-Guevara, Mirna Vázquez-Villamar, Karla Fernández-Acosta, Mayra Yanet Méndez-Gómez, Isela Parra-Rojas, Norma Samanta Romero-Castro
{"title":"Analysis of subgingival microbiota and IL-1β, TNF-α and CX3CL1 levels in gingival crevicular fluid of fixed dental prostheses.","authors":"Mario Alberto Alarcón-Sánchez, Natividad Castro-Alarcón, Daniel Sandoval-Guevara, Mirna Vázquez-Villamar, Karla Fernández-Acosta, Mayra Yanet Méndez-Gómez, Isela Parra-Rojas, Norma Samanta Romero-Castro","doi":"10.4012/dmj.2023-136","DOIUrl":"10.4012/dmj.2023-136","url":null,"abstract":"<p><p>Prosthetic biomaterials can affect the composition of the subgingival microbiota and consequently the production of proinflammatory cytokines, causing damage to the periodontium. A total of 40 patients were divided into two groups: 20 with monolithic zirconia (MZ) prostheses and 20 with porcelain fused to metal (PFM) with nickel-chromium (Ni-Cr) alloy prostheses. Subgingival plaque and gingival crevicular fluid samples were taken. The Checkerboard technique for DNA-DNA hybridization and the enzyme-linked immunosorbent assay technique were performed. Teeth with MZ presented a lower percentage of bleeding on probing and tooth mobility compared to teeth with PFM with Ni-Cr alloy. Prosthodontic teeth harbored higher total levels of the 18 bacterial species than non-prosthodontic teeth. There was a higher prevalence of S. gordonii and V. parvula species in PFM with Ni-Cr alloy compared to MZ. There was an increase in IL-1β, TNF-α and CX3CL1 levels in PFM with Ni-Cr alloy compared to MZ. MZ is a candidate biomaterial with fewer negative effects on the periodontium, allowing for longer prostheses longevity in the mouth.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139989583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gitae Son, Gyeung Mi Seon, Sang Hoon Choi, Hyeong-Cheol Yang
{"title":"Effects of vehicles on the physical properties and biocompatibility of premixed calcium silicate cements.","authors":"Gitae Son, Gyeung Mi Seon, Sang Hoon Choi, Hyeong-Cheol Yang","doi":"10.4012/dmj.2023-147","DOIUrl":"10.4012/dmj.2023-147","url":null,"abstract":"<p><p>Premixed calcium silicate cements (pCSCs) contain vehicles which endow fluidity and viscosity to CSCs. This study aimed to investigate the effects of three vehicles, namely, polyethylene glycol (PEG), propylene glycol (PG), and dimethyl sulfoxide (DMSO), on the physicochemical properties and biocompatibility of pCSCs. The setting time, solubility, expansion rate, and mechanical strength of the pCSCs were evaluated, and the formation of calcium phosphate precipitates was assessed in phosphate-buffered saline (PBS). The effects of pCSC extracts on the osteogenic differentiation of mesenchymal stem cells (MSCs) were investigated. Finally, the tissue compatibility of pCSCs in rat femurs was observed. CSC containing PEG (CSC-PEG) exhibited higher solubility and setting time, and CSC-DMSO showed the highest expansion rate and mechanical strength. All pCSCs generated calcium phosphate precipitates. The extract of CSC-PG induced the highest expressions of osteogenic markers along with the greatest calcium deposites. When implanted in rat femurs, CSC-PEG was absorbed considerably, whereas CSC-PG remained relatively unaltered inside the femur.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140049038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Exploration of reference genes for the development of a diagnostic kit on vascular aging in human saliva.","authors":"Shinsuke Miyoshi, Akiyo Kawamoto, Yuichi Ninomiya, Yoshihiro Hamada, Hideo Shimizu, Yoshitomo Honda, Kazuya Takahashi","doi":"10.4012/dmj.2023-242","DOIUrl":"10.4012/dmj.2023-242","url":null,"abstract":"<p><p>Identifying reliable biomarkers in saliva can be a promising approach to developing a rapid diagnostic kit for detecting vascular aging. This study investigated the most suitable reference gene for polymerase chain reaction (PCR) in saliva that is not affected by vascular aging variables. Whole saliva samples were collected to assess the expression of reference genes: actin beta (ACTB), 18S ribosomal RNA (18S rRNA), beta-2-microglobulin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The most abundantly expressed gene was 18S rRNA, and the least expressed gene was GAPDH. Four genes were ranked according to their relative stability, as determined by mathematical algorithms, indicating that ACTB and 18S rRNA were stably expressed as reference genes. 18S rRNA was identified as the most promising reference gene for detecting systemic diseases using saliva from patients with vascular aging in these limited experimental conditions.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139511837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soo-Auk Park, Julee Son, Ah-Jin Kim, Seunghan Oh, Ji-Myung Bae
{"title":"Effect of adhesive components in experimental fluoride varnish on fluoride release within 30 days in vitro study.","authors":"Soo-Auk Park, Julee Son, Ah-Jin Kim, Seunghan Oh, Ji-Myung Bae","doi":"10.4012/dmj.2023-299","DOIUrl":"10.4012/dmj.2023-299","url":null,"abstract":"<p><p>We aimed to determine whether adhesive components could increase the release time of effective fluoride concentration from an experimental fluoride varnish applied to bovine teeth. An experimental fluoride varnish containing 5% sodium fluoride (EX1) was prepared and combined with 35% hydroxyethyl methacrylate (HEMA) (EX2), 5% glutaraldehyde (EX3), or 35% HEMA/5% glutaraldehyde mixture (EX4). Two commercially available fluoride varnishes were used for comparison. Each group was applied to bovine incisors, and the fluoride release and pH were monitored for 30 days. Cell viability analysis, scanning electron microscopy, and energy-dispersive spectroscopy were performed. EX4 released the highest and most effective concentration of fluoride for the longest period and reached neutral pH at the earliest; the release was maintained for up to 30 days without cytotoxicity. In conclusion, EX4 is considered to be the most effective varnish to prevent dental caries.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139930466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Changes in enamel hardness, wear resistance, surface texture, and surface crystal structure with glass ionomer cement containing BioUnion fillers.","authors":"Misuzu Sagawa, Yasuhiro Namura, Yasuki Uchida, Wakako Miyama, Shirabe Nishimura, Toshihiro Yoneyama, Toshiki Takamizawa, Mitsuru Motoyoshi","doi":"10.4012/dmj.2023-180","DOIUrl":"10.4012/dmj.2023-180","url":null,"abstract":"<p><p>This study investigated the potential of BioUnion filler containing glass ionomer cement (GIC) to enhance the properties of enamel surrounding restorations, with a specific focus on the effect on hardness. The hardness of the bovine enamel immersed in the cement was measured using Vickers hardness numbers. Following sliding and impact wear simulations, the enamel facets were examined using confocal-laser-scanning microscopy and scanning-electron microscopy. Surface properties were further analyzed using energy-dispersive X-ray spectroscopy and X-ray diffraction (XRD). A significant increase in Vickers hardness numbers was observed in the BioUnion filler GIC after 2 days. Furthermore, the mean depth of enamel facets treated with BioUnion filler GIC was significantly less than that of untreated facets. Characteristic XRD peaks indicating the presence of hydroxyapatite were also observed. Our findings imply that GIC with BioUnion fillers enhances the mechanical properties of the tooth surface adjacent to the cement.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139930465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biocompatibility of dental implants coated with hydroxyapatite using pulsed Er:YAG laser deposition.","authors":"Yuri Kuki, Kenzo Morinaga, Naoya Uemura, Tomoharu Okamura, Shigeki Hontsu, Yoshiya Hashimoto, Shunsuke Baba","doi":"10.4012/dmj.2023-235","DOIUrl":"10.4012/dmj.2023-235","url":null,"abstract":"<p><p>We aimed to improve the biocompatibility and osteoinductive potential of Ti implants using a simulated intraoral hydroxyapatite (HAp) coating. We devised a novel surface treatment for aggressive induction of osteoblast adhesion and bone regeneration on the implant surface. A thin α-tricalcium phosphate (α-TCP) film was deposited on the implant surface using a pulsed Er:YAG laser. The coating was converted to HAp through artificial saliva immersion, which was confirmed using scanning electron microscopy (SEM) and X-ray diffraction (XRD). SEM showed needle-like HAp crystals on the Ti disks and sandblasted implant surfaces after immersion in artificial saliva for 96 h. Microcomputed tomography and histological evaluation 4 and 8 weeks after implantation into beagle dog mandibles showed that the HAp-coated implant was biocompatible and exhibited superior osteoinduction compared to that of sandblasted implants. Coating the implant surface with HAp using an Er:YAG laser has potential as a new method of the implant-surface debridement.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139989584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yusuke Tatsumi, Harumi Kawaki, Kohei Shintani, Kyohei Ueno, Masato Hotta, Nobuo Kondoh, Michael F Burrow, Toru Nikaido
{"title":"Bioactivity of human dental pulp-derived stem cells with boron-controlled S-PRG filler eluate by anion exchange.","authors":"Yusuke Tatsumi, Harumi Kawaki, Kohei Shintani, Kyohei Ueno, Masato Hotta, Nobuo Kondoh, Michael F Burrow, Toru Nikaido","doi":"10.4012/dmj.2023-145","DOIUrl":"10.4012/dmj.2023-145","url":null,"abstract":"<p><p>Surface pre-reacted glass-ionomer (S-PRG) filler is a bioactive glass filler capable of releasing various ions. A culture medium to which was added an S-PRG filler eluate rich in boron was reported to enhance alkaline phosphatase (ALP) activity in human dental pulp-derived stem cells (hDPSC). To clarify the role of boron eluted from S-PRG fillers, the modified S-PRG filler eluate with different boron concentrations was prepared by using an anion exchange material. Therefore, elemental mapping analysis of anion exchange material, adsorption ratio, hDPSCs proliferation and ALP activity were evaluated. For statistical analysis, Kruskal-Wallis test was used, with statistical significance determined at p<0.05. ALP activity enhancement was not observed in hDPSC cultured in the medium that contained the S-PRG filler eluate from which boron had been removed. The result suggested the possibility that an S-PRG filler eluate with controlled boron release could be useful for the development of novel dental materials.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140021174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}