{"title":"Exploration of reference genes for the development of a diagnostic kit on vascular aging in human saliva.","authors":"Shinsuke Miyoshi, Akiyo Kawamoto, Yuichi Ninomiya, Yoshihiro Hamada, Hideo Shimizu, Yoshitomo Honda, Kazuya Takahashi","doi":"10.4012/dmj.2023-242","DOIUrl":"10.4012/dmj.2023-242","url":null,"abstract":"<p><p>Identifying reliable biomarkers in saliva can be a promising approach to developing a rapid diagnostic kit for detecting vascular aging. This study investigated the most suitable reference gene for polymerase chain reaction (PCR) in saliva that is not affected by vascular aging variables. Whole saliva samples were collected to assess the expression of reference genes: actin beta (ACTB), 18S ribosomal RNA (18S rRNA), beta-2-microglobulin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The most abundantly expressed gene was 18S rRNA, and the least expressed gene was GAPDH. Four genes were ranked according to their relative stability, as determined by mathematical algorithms, indicating that ACTB and 18S rRNA were stably expressed as reference genes. 18S rRNA was identified as the most promising reference gene for detecting systemic diseases using saliva from patients with vascular aging in these limited experimental conditions.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":" ","pages":"172-178"},"PeriodicalIF":2.5,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139511837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soo-Auk Park, Julee Son, Ah-Jin Kim, Seunghan Oh, Ji-Myung Bae
{"title":"Effect of adhesive components in experimental fluoride varnish on fluoride release within 30 days in vitro study.","authors":"Soo-Auk Park, Julee Son, Ah-Jin Kim, Seunghan Oh, Ji-Myung Bae","doi":"10.4012/dmj.2023-299","DOIUrl":"10.4012/dmj.2023-299","url":null,"abstract":"<p><p>We aimed to determine whether adhesive components could increase the release time of effective fluoride concentration from an experimental fluoride varnish applied to bovine teeth. An experimental fluoride varnish containing 5% sodium fluoride (EX1) was prepared and combined with 35% hydroxyethyl methacrylate (HEMA) (EX2), 5% glutaraldehyde (EX3), or 35% HEMA/5% glutaraldehyde mixture (EX4). Two commercially available fluoride varnishes were used for comparison. Each group was applied to bovine incisors, and the fluoride release and pH were monitored for 30 days. Cell viability analysis, scanning electron microscopy, and energy-dispersive spectroscopy were performed. EX4 released the highest and most effective concentration of fluoride for the longest period and reached neutral pH at the earliest; the release was maintained for up to 30 days without cytotoxicity. In conclusion, EX4 is considered to be the most effective varnish to prevent dental caries.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":" ","pages":"320-327"},"PeriodicalIF":2.5,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139930466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Changes in enamel hardness, wear resistance, surface texture, and surface crystal structure with glass ionomer cement containing BioUnion fillers.","authors":"Misuzu Sagawa, Yasuhiro Namura, Yasuki Uchida, Wakako Miyama, Shirabe Nishimura, Toshihiro Yoneyama, Toshiki Takamizawa, Mitsuru Motoyoshi","doi":"10.4012/dmj.2023-180","DOIUrl":"10.4012/dmj.2023-180","url":null,"abstract":"<p><p>This study investigated the potential of BioUnion filler containing glass ionomer cement (GIC) to enhance the properties of enamel surrounding restorations, with a specific focus on the effect on hardness. The hardness of the bovine enamel immersed in the cement was measured using Vickers hardness numbers. Following sliding and impact wear simulations, the enamel facets were examined using confocal-laser-scanning microscopy and scanning-electron microscopy. Surface properties were further analyzed using energy-dispersive X-ray spectroscopy and X-ray diffraction (XRD). A significant increase in Vickers hardness numbers was observed in the BioUnion filler GIC after 2 days. Furthermore, the mean depth of enamel facets treated with BioUnion filler GIC was significantly less than that of untreated facets. Characteristic XRD peaks indicating the presence of hydroxyapatite were also observed. Our findings imply that GIC with BioUnion fillers enhances the mechanical properties of the tooth surface adjacent to the cement.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":" ","pages":"247-254"},"PeriodicalIF":2.5,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139930465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biocompatibility of dental implants coated with hydroxyapatite using pulsed Er:YAG laser deposition.","authors":"Yuri Kuki, Kenzo Morinaga, Naoya Uemura, Tomoharu Okamura, Shigeki Hontsu, Yoshiya Hashimoto, Shunsuke Baba","doi":"10.4012/dmj.2023-235","DOIUrl":"10.4012/dmj.2023-235","url":null,"abstract":"<p><p>We aimed to improve the biocompatibility and osteoinductive potential of Ti implants using a simulated intraoral hydroxyapatite (HAp) coating. We devised a novel surface treatment for aggressive induction of osteoblast adhesion and bone regeneration on the implant surface. A thin α-tricalcium phosphate (α-TCP) film was deposited on the implant surface using a pulsed Er:YAG laser. The coating was converted to HAp through artificial saliva immersion, which was confirmed using scanning electron microscopy (SEM) and X-ray diffraction (XRD). SEM showed needle-like HAp crystals on the Ti disks and sandblasted implant surfaces after immersion in artificial saliva for 96 h. Microcomputed tomography and histological evaluation 4 and 8 weeks after implantation into beagle dog mandibles showed that the HAp-coated implant was biocompatible and exhibited superior osteoinduction compared to that of sandblasted implants. Coating the implant surface with HAp using an Er:YAG laser has potential as a new method of the implant-surface debridement.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":" ","pages":"269-275"},"PeriodicalIF":2.5,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139989584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yusuke Tatsumi, Harumi Kawaki, Kohei Shintani, Kyohei Ueno, Masato Hotta, Nobuo Kondoh, Michael F Burrow, Toru Nikaido
{"title":"Bioactivity of human dental pulp-derived stem cells with boron-controlled S-PRG filler eluate by anion exchange.","authors":"Yusuke Tatsumi, Harumi Kawaki, Kohei Shintani, Kyohei Ueno, Masato Hotta, Nobuo Kondoh, Michael F Burrow, Toru Nikaido","doi":"10.4012/dmj.2023-145","DOIUrl":"10.4012/dmj.2023-145","url":null,"abstract":"<p><p>Surface pre-reacted glass-ionomer (S-PRG) filler is a bioactive glass filler capable of releasing various ions. A culture medium to which was added an S-PRG filler eluate rich in boron was reported to enhance alkaline phosphatase (ALP) activity in human dental pulp-derived stem cells (hDPSC). To clarify the role of boron eluted from S-PRG fillers, the modified S-PRG filler eluate with different boron concentrations was prepared by using an anion exchange material. Therefore, elemental mapping analysis of anion exchange material, adsorption ratio, hDPSCs proliferation and ALP activity were evaluated. For statistical analysis, Kruskal-Wallis test was used, with statistical significance determined at p<0.05. ALP activity enhancement was not observed in hDPSC cultured in the medium that contained the S-PRG filler eluate from which boron had been removed. The result suggested the possibility that an S-PRG filler eluate with controlled boron release could be useful for the development of novel dental materials.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":" ","pages":"255-262"},"PeriodicalIF":2.5,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140021174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative bonding performance of coronal dentin disks and CAD/CAM resin composite disks for biological restoration: The impact of resin-coating technique.","authors":"Hiroki Ishihara, Kazuhide Yonekura, Masaomi Ikeda, Go Inoue, Masatoshi Nakajima, Yasushi Shimada, Keiichi Hosaka","doi":"10.4012/dmj.2023-229","DOIUrl":"10.4012/dmj.2023-229","url":null,"abstract":"<p><p>This study evaluated the bonding performance of coronal dentin disks, designed for biological restoration, and CAD/CAM resin composite disks when bonded to flat dentin surfaces using dual-cure resin cements, with and without a resin-coating (RC) technique. Three distinct groups were established within the non-RC group, each using one of the two types of resin cements in a self-adhesive mode: one-step self-etch adhesive (1-SEA) without light-cure, 1-SEA with light-cure, and a separate group using an alternate cement. Within the RC group, a subgroup was established for each cement. The microtensile bond strength (μTBS) of the disk-dentin beam was tested after 0 and 10,000 thermocycles in a 5°C/55°C. No significant μTBS difference was observed among the non-RC groups. However, when using RC, the μTBSs of coronal dentin disks significantly exceeded those of CAD/CAM resin composite disks. Thermocycle aging did not affect μTBS in any of the bonding methods, except in self-adhesive mode.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":" ","pages":"303-311"},"PeriodicalIF":2.5,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140049037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of low-temperature degradation and sintering protocols on the color of monolithic zirconia crowns with different yttria contents.","authors":"Shoko Miura, Akikazu Shinya, Hiroyasu Koizumi, Pekka Vallittu, Lippo Lassila, Masanori Fujisawa","doi":"10.4012/dmj.2023-194","DOIUrl":"10.4012/dmj.2023-194","url":null,"abstract":"<p><p>This study investigated the effects of low-temperature degradation (LTD) on the L*, a*, and b* values of highly translucent zirconia crowns. Four types of zirconia disks with different yttria contents (IPS e.max ZirCAD LT, IPS e.max ZirCAD MT, IPS e.max ZirCAD MT Multi, IPS e.max ZirCAD Prime, Ivoclar) and two shades (A2 and BL) were used. A crown was manufactured using four types of zirconia and LTD treated. Color measurements were performed, and the color difference (ΔE<sub>00</sub>) before and after LTD was calculated. The microstructure was determined through X-ray fluorescence and X-ray diffractometry. Highly translucent zirconia crowns showed greater changes in the a* and b* values than in the L* value after LTD, regardless of the shade. The Multi2 crowns exhibited a discernible color change due to the LTD treatment. The X-ray fluorescence results did not reveal any apparent change in the microstructure between sintering programs for all zirconia specimens.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":" ","pages":"164-171"},"PeriodicalIF":2.5,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of wear and marginal fitness of 3D-printed deciduous molar crowns: An in vitro study.","authors":"Bin Lei, Huacui Xiong, Ke Chen","doi":"10.4012/dmj.2022-143","DOIUrl":"10.4012/dmj.2022-143","url":null,"abstract":"<p><p>This study aimed to evaluate the wear resistance of primary tooth enamel and 3 kinds of 3D printing materials and to compare the marginal fitness and internal suitability of prefabricated all-ceramic crowns, computer-aided design/manufacturing (CAD/CAM) all-ceramic crowns, and three 3D-printed deciduous molar crowns. Multifunctional friction wear testing machine was used to image the wear surface of the sample and calculate the maximum wear depth and volume loss value of each sample. The internal fit evaluation used the silicon replica method, The four points were measured using scanning electron microscopy (SEM). The obtained data were statistically analyzed using ANOVA and Tukey HSD-test with a fully randomized design (p<0.05). The results showed the wear resistance of E-Dent400 was better than that of PEEK and three different 3D printed materials have good wear resistance compared with the primary tooth enamel. The measured values at M1 and M4 of E-Dent400 were both the smallest.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":" ","pages":"227-234"},"PeriodicalIF":2.5,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139989585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fumika Abe, Ayana Nakano, Isao Hirata, Kotaro Tanimoto, Koichi Kato
{"title":"Structure and function of engineered stromal cell-derived factor-1α.","authors":"Fumika Abe, Ayana Nakano, Isao Hirata, Kotaro Tanimoto, Koichi Kato","doi":"10.4012/dmj.2023-247","DOIUrl":"10.4012/dmj.2023-247","url":null,"abstract":"<p><p>To design biologically active, collagen-based scaffolds for bone tissue engineering, we have synthesized chimeric proteins consisting of stromal cell-derived factor-1α (SDF) and the von Willebrand factor A3 collagen-binding domain (CBD). The chimeric proteins were used to evaluate the effect of domain linkage and its order on the structure and function of the SDF and CBD. The structure of the chimeric proteins was analyzed by circular dichroism spectroscopy, while functional analysis was performed by a cell migration assay for the SDF domain and a collagen-binding assay for the CBD domain. Furthermore, computational structural prediction was conducted for the chimeric proteins to examine the consistency with the results of structural and functional analyses. Our structural and functional analyses as well as structural prediction revealed that linking two domains can affect their functions. However, their order had minor effects on the three-dimensional structure of CBD and SDF in the chimeric proteins.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":" ","pages":"286-293"},"PeriodicalIF":2.5,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139989586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fracture strength of porcelain veneer on surface-treated zirconia.","authors":"Hiraku Onuma, Masanao Inokoshi, Kaiqi Xu, Shunsuke Minakuchi","doi":"10.4012/dmj.2023-139","DOIUrl":"10.4012/dmj.2023-139","url":null,"abstract":"<p><p>In this study, we investigated the effects of surface treatment on the fracture strength of porcelain-veneered zirconia. Highly translucent 4 mol% yttria-stabilized zirconia disks (KATANA HT, Kuraray Noritake Dental) were divided into three surface-treatment groups: 1)as-sintered, 2) alumina sandblasted, and 3) ground. Crystallographic and surface-roughness analyses were conducted for each group. Veneering ceramics (Cerabien ZR, Kuraray Noritake Dental) were applied to the zirconia surfaces. The fracture strengths of the porcelain-veneered zirconia disks were measured using biaxial flexural-strength tests. Crystallographic analysis revealed that grinding and sandblasting increased the fractions of the monoclinic and rhombohedral zirconia phases. The ground specimens had a higher surface roughness than the sandblasted specimens. Weibull analysis showed no significant differences in biaxial flexural strength among the three groups. The results suggest that these surface treatments do not affect the fracture strength of porcelain-veneered zirconia.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":" ","pages":"263-268"},"PeriodicalIF":2.5,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139930467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}