DNA fragmentation of lymphocytes and sperm cells induced by nickel released from orthodontic archwires: A preliminary study.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-31 Epub Date: 2024-07-04 DOI:10.4012/dmj.2023-286

José Alfredo Facio Umaña, Roberto Beltran Del Rio Parra, Raúl Uriel Medina Martínez, Nadia Betancourt Martínez, Pedro Iv González Luna, Javier Morán Martínez

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Abstract

Orthodontic brackets and archwires placed intraorally are subject to corrosion, leading to the release of cytotoxic metal ions. The aim of this study was to determine whether the use of orthodontic NiTi archwires increases systemic Ni levels and cause alterations on the DNA of cells unrelated to the oral environment such as lymphocytes and sperm cells. Human urine, semen and blood samples were collected before (baseline) sham placement of orthodontic archwires and 15 and 30 days after placement. Lymphocytes and sperm cells cells were evaluated by comet assay. Ni concentration levels in urine increased significantly between baseline and 15 days (p<0.01) and 15 and 30 days of exposure (p<0.01). Progressive decrease in sperm viability and motility was observed between the sampling periods. Lymphocytes and sperm cells showed DNA fragmentation. The increase in systemic concentration of nickel induced structural damage in the DNA of lymphocytes and human sperm cells.

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正畸弓丝释放的镍诱导淋巴细胞和精子细胞的 DNA 断裂：初步研究

口腔内放置的正畸托槽和弓丝会受到腐蚀，从而释放出具有细胞毒性的金属离子。本研究的目的是确定使用正畸镍钛弓丝是否会增加全身的镍含量，并对淋巴细胞和精子细胞等与口腔环境无关的细胞的 DNA 造成改变。在假性安装正畸弓丝前（基线）、安装后 15 天和 30 天收集人体尿液、精液和血液样本。通过彗星试验对淋巴细胞和精子细胞进行了评估。尿液中的镍浓度水平在基线和 15 天之间明显增加（p

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.