José Alfredo Facio Umaña, Roberto Beltran Del Rio Parra, Raúl Uriel Medina Martínez, Nadia Betancourt Martínez, Pedro Iv González Luna, Javier Morán Martínez
{"title":"正畸弓丝释放的镍诱导淋巴细胞和精子细胞的 DNA 断裂:初步研究","authors":"José Alfredo Facio Umaña, Roberto Beltran Del Rio Parra, Raúl Uriel Medina Martínez, Nadia Betancourt Martínez, Pedro Iv González Luna, Javier Morán Martínez","doi":"10.4012/dmj.2023-286","DOIUrl":null,"url":null,"abstract":"<p><p>Orthodontic brackets and archwires placed intraorally are subject to corrosion, leading to the release of cytotoxic metal ions. The aim of this study was to determine whether the use of orthodontic NiTi archwires increases systemic Ni levels and cause alterations on the DNA of cells unrelated to the oral environment such as lymphocytes and sperm cells. Human urine, semen and blood samples were collected before (baseline) sham placement of orthodontic archwires and 15 and 30 days after placement. Lymphocytes and sperm cells cells were evaluated by comet assay. Ni concentration levels in urine increased significantly between baseline and 15 days (p<0.01) and 15 and 30 days of exposure (p<0.01). Progressive decrease in sperm viability and motility was observed between the sampling periods. Lymphocytes and sperm cells showed DNA fragmentation. The increase in systemic concentration of nickel induced structural damage in the DNA of lymphocytes and human sperm cells.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":" ","pages":"539-545"},"PeriodicalIF":1.9000,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"DNA fragmentation of lymphocytes and sperm cells induced by nickel released from orthodontic archwires: A preliminary study.\",\"authors\":\"José Alfredo Facio Umaña, Roberto Beltran Del Rio Parra, Raúl Uriel Medina Martínez, Nadia Betancourt Martínez, Pedro Iv González Luna, Javier Morán Martínez\",\"doi\":\"10.4012/dmj.2023-286\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Orthodontic brackets and archwires placed intraorally are subject to corrosion, leading to the release of cytotoxic metal ions. The aim of this study was to determine whether the use of orthodontic NiTi archwires increases systemic Ni levels and cause alterations on the DNA of cells unrelated to the oral environment such as lymphocytes and sperm cells. Human urine, semen and blood samples were collected before (baseline) sham placement of orthodontic archwires and 15 and 30 days after placement. Lymphocytes and sperm cells cells were evaluated by comet assay. Ni concentration levels in urine increased significantly between baseline and 15 days (p<0.01) and 15 and 30 days of exposure (p<0.01). Progressive decrease in sperm viability and motility was observed between the sampling periods. Lymphocytes and sperm cells showed DNA fragmentation. The increase in systemic concentration of nickel induced structural damage in the DNA of lymphocytes and human sperm cells.</p>\",\"PeriodicalId\":11065,\"journal\":{\"name\":\"Dental materials journal\",\"volume\":\" \",\"pages\":\"539-545\"},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2024-07-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Dental materials journal\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.4012/dmj.2023-286\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/7/4 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Dental materials journal","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.4012/dmj.2023-286","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/7/4 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 0
摘要
口腔内放置的正畸托槽和弓丝会受到腐蚀,从而释放出具有细胞毒性的金属离子。本研究的目的是确定使用正畸镍钛弓丝是否会增加全身的镍含量,并对淋巴细胞和精子细胞等与口腔环境无关的细胞的 DNA 造成改变。在假性安装正畸弓丝前(基线)、安装后 15 天和 30 天收集人体尿液、精液和血液样本。通过彗星试验对淋巴细胞和精子细胞进行了评估。尿液中的镍浓度水平在基线和 15 天之间明显增加(p
DNA fragmentation of lymphocytes and sperm cells induced by nickel released from orthodontic archwires: A preliminary study.
Orthodontic brackets and archwires placed intraorally are subject to corrosion, leading to the release of cytotoxic metal ions. The aim of this study was to determine whether the use of orthodontic NiTi archwires increases systemic Ni levels and cause alterations on the DNA of cells unrelated to the oral environment such as lymphocytes and sperm cells. Human urine, semen and blood samples were collected before (baseline) sham placement of orthodontic archwires and 15 and 30 days after placement. Lymphocytes and sperm cells cells were evaluated by comet assay. Ni concentration levels in urine increased significantly between baseline and 15 days (p<0.01) and 15 and 30 days of exposure (p<0.01). Progressive decrease in sperm viability and motility was observed between the sampling periods. Lymphocytes and sperm cells showed DNA fragmentation. The increase in systemic concentration of nickel induced structural damage in the DNA of lymphocytes and human sperm cells.
期刊介绍:
Dental Materials Journal is a peer review journal published by the Japanese Society for Dental Materials and Devises aiming to introduce the progress of the basic and applied sciences in dental materials and biomaterials. The dental materials-related clinical science and instrumental technologies are also within the scope of this journal. The materials dealt include synthetic polymers, ceramics, metals and tissue-derived biomaterials. Forefront dental materials and biomaterials used in developing filed, such as tissue engineering, bioengineering and artificial intelligence, are positively considered for the review as well. Recent acceptance rate of the submitted manuscript in the journal is around 30%.