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International Conference on Photonics and Imaging in Biology and Medicine最新文献

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Cellular rehabilitation of photobiomodulation 光生物调节的细胞康复
International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741093
T. Liu, Jian-Qin Yuan, Yan-Fang Wang, Xiao-Yang Xu, Song-hao Liu
{"title":"Cellular rehabilitation of photobiomodulation","authors":"T. Liu, Jian-Qin Yuan, Yan-Fang Wang, Xiao-Yang Xu, Song-hao Liu","doi":"10.1117/12.741093","DOIUrl":"https://doi.org/10.1117/12.741093","url":null,"abstract":"Homeostasis is a term that refers to constancy in a system. A cell in homeostasis normally functions. There are two kinds of processes in the internal environment and external environment of a cell, the pathogenic processes (PP) which disrupts the old homeostasis (OH), and the sanogenetic processes (SP) which restores OH or establishes a new homeostasis (NH). Photobiomodualtion (PBM), the cell-specific effects of low intensity monochromatic light or low intensity laser irradiation (LIL) on biological systems, is a kind of modulation on PP or SP so that there is no PBM on a cell in homeostasis. There are two kinds of pathways mediating PBM, the membrane endogenetic chromophores mediating pathways which often act through reactive oxygen species, and membrane proteins mediating pathways which often enhance cellular SP so that it might be called cellular rehabilitation. The cellular rehabilitation of PBM will be discussed in this paper. It is concluded that PBM might modulate the disruption of cellular homeostasis induced by pathogenic factors such as toxin until OH has been restored or NH has been established, but can not change homeostatic processes from one to another one.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126518383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Distinguish activations on sensorimotor cortex using high-resolution diffuse optical tomography 使用高分辨率漫射光学断层扫描区分感觉运动皮层的激活
International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741097
Qing Zhao, Lijun Ji, Feng Shi, T. Jiang
{"title":"Distinguish activations on sensorimotor cortex using high-resolution diffuse optical tomography","authors":"Qing Zhao, Lijun Ji, Feng Shi, T. Jiang","doi":"10.1117/12.741097","DOIUrl":"https://doi.org/10.1117/12.741097","url":null,"abstract":"Diffuse optical tomography (DOT) is an appropriate tool for non-invasive exploration of human brain activation. The activation of sensorimotor cortex has been studied by several researchers since the first images of human brain were generated in 1995. However, high-quality images of sensorimotor cortex can not be obtained until the emerging of high-resolution DOT which uses a multi-centered geometry for arranging optical fibers. In this study, we did two experiments using our CW5 instrument (TechEn, USA). In the first experiment, the subject was asked to move his four fingers of right hand for 30 seconds, followed by 30 seconds of rest. Data collection lasted 420 seconds. In the second experiment, the subject was asked to tap his thumb against the other four fingers. Two conclusions can be reached from the experiments. Firstly, larger activated regions can be found on motor cortex in experiment 2 than in experiment 1. This indicates that high-resolution DOT can detect larger activated brain region when moving five fingers comparing to moving four fingers. Secondly, few activated regions can be found on sensory cortex in experiment 1, but it can be clearly found on sensory cortex in experiment 2. Up to our knowledge, it is the first time DOT has detected activated region on sensory cortex during motorial task.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128977276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Up-converting phosphor technology-based biosensor 基于上转换荧光粉技术的生物传感器
International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741620
Kun Yu, Lei Zhou, Lei Liu, Lihua Huang, Zhongqiang Yan, Yongkai Zhao, Ruifu Yang, Huijie Huang
{"title":"Up-converting phosphor technology-based biosensor","authors":"Kun Yu, Lei Zhou, Lei Liu, Lihua Huang, Zhongqiang Yan, Yongkai Zhao, Ruifu Yang, Huijie Huang","doi":"10.1117/12.741620","DOIUrl":"https://doi.org/10.1117/12.741620","url":null,"abstract":"A new generation of Up-converting phosphor technology-based biosensors (UPT-based biosensor) has been developed for immunoassay, where a kind of novel Up-converting phosphor (UCP) particle serves as the biological marker. Its control system is based on a micro-computer control unit instead of a personal computer, which results in low power consumption, high reliability and portability for field detection. By detecting the content of the UCP on the test strip, the system figures out the concentration of the biological molecular of interest, which can be used in field examination of biological analytes. In this paper, a series concentration of standard samples have been tested by the biosensor, which is proved to have higher sensitivity (~ng/ml), higher stability (CV<3~5%), better linear relationship and an excellent correlation (R2≥0.95). The UPT-based biosensor has stable, reliable and sensitive performances. It can meet the need of various rapid bioassay applications.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133625346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Light scattering by biological spheroidal particles in geometric optics approximation 几何光学近似中生物球状粒子的光散射
International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741344
A. Lugovtsov, A. Priezzhev, S. Nikitin
{"title":"Light scattering by biological spheroidal particles in geometric optics approximation","authors":"A. Lugovtsov, A. Priezzhev, S. Nikitin","doi":"10.1117/12.741344","DOIUrl":"https://doi.org/10.1117/12.741344","url":null,"abstract":"The main goal of this work is the fast analysis of light scattered by a large optically soft spheroidal particle (size parameter ka > 20). In particular, these particles mimic single cells (e.g., red blood cells (RBC)) and their aggregates. Methodology of calculation of light scattering by arbitrarily oriented optically soft spheroidal particles in geometric optics approximation is presented. We show that the geometric optics approximation permits to quickly calculate the angular distributions of the intensities and phases of light scattered from optically soft particles, such as erythrocytes.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"6534 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131228331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Single dye molecules observed by total internal reflection fluorescence microscopy 用全内反射荧光显微镜观察单个染料分子
International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741569
Jinyuan Wang, Guo Fu, Chen Wang, Li Liu, Gui-ying Wang
{"title":"Single dye molecules observed by total internal reflection fluorescence microscopy","authors":"Jinyuan Wang, Guo Fu, Chen Wang, Li Liu, Gui-ying Wang","doi":"10.1117/12.741569","DOIUrl":"https://doi.org/10.1117/12.741569","url":null,"abstract":"Total internal reflection fluorescence microscopy (TIRFM) was used to image single molecules with evanescent waves. The molecules were excited by an evanescent wave with different intensities. Single molecules were imaged on low-noise high-quantum-yield charge-coupled device (CCD) cameras. Two-step photobleaching behavior was observed. Duration from fluorescent spots appearing to disappearing was counted. The duration was decided by the speed of photobleaching. Peak intensity was counted as exciting intensity change. The proportion relationship between the reciprocal of duration and exciting intensity was obtained. The emitted intensity and duration of fluorescein were compared with GFP. A single molecules emit the same number photons was proved.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132817453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expanded research of spectroscopic optical tomographic functional imaging system 光谱光学层析功能成像系统的扩展研究
International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741360
Yuheng Chen, Z. Ding, J. Meng
{"title":"Expanded research of spectroscopic optical tomographic functional imaging system","authors":"Yuheng Chen, Z. Ding, J. Meng","doi":"10.1117/12.741360","DOIUrl":"https://doi.org/10.1117/12.741360","url":null,"abstract":"Spectroscopic optical coherence tomography (OCT), a functional extension of conventional structural OCT is developed based on previous fiber-based OCT system. Spectral information is obtained by carrying out Fourier transformation of the detected OCT signal without recurring to hardware. The developed system is applied to film reflectivity measurement and spectroscopic imaging of a finger from a human volunteer. The feasibility for spectroscopic extension of the developed system is confirmed by comparison curves of film reflectivity measured by the developed system with that obtained from a commercial spectrometer.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134423290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Data and image processing for a simultaneous time-and spectrum-resolved multifocal multiphoton microscopy 同时时间和光谱分辨多焦点多光子显微镜的数据和图像处理
International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741586
Z. Fu, J. Qu, Zi-yang Lin, Lixin Liu, B. Guo, H. Niu
{"title":"Data and image processing for a simultaneous time-and spectrum-resolved multifocal multiphoton microscopy","authors":"Z. Fu, J. Qu, Zi-yang Lin, Lixin Liu, B. Guo, H. Niu","doi":"10.1117/12.741586","DOIUrl":"https://doi.org/10.1117/12.741586","url":null,"abstract":"A Simultaneous Time- and Spectrum-Resolved Multifocal Multiphoton Microscopy (STSR-MMM) system has been developed for the acquisition of fluorescence lifetime information of any spectrum of the biological samples in only one measurement. The near-infrared light from mode-locked titanium: sapphire femtosecond laser is split with a microlens array into an array of beams that are transformed into an array of high-aperture foci at the sample for parallel multiphoton excitation. The Gaussian shape of the excitation beam results in non-uniformity and asymmetry of the excitation foci array and also of the reconstructed fluorescence image. A coefficient matrix has been presented for the correction of the non-uniformity and asymmetry in the fluorescence image. Data acquisition and image processing methods have also been investigated to increase the image acquisition speed and improve the reconstruction of time- and spectrum-resolved fluorescence image. The methods for obtaining fluorescence intensity image, spectrally resolved intensity image and spectrally resolved lifetime image are also presented.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131768482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ultrahigh resolution parallel Fourier domain optical coherence tomography using xenon flash lamp 氙闪光灯超高分辨率平行傅里叶域光学相干层析成像
International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741362
Xuan Liu, P. Xue
{"title":"Ultrahigh resolution parallel Fourier domain optical coherence tomography using xenon flash lamp","authors":"Xuan Liu, P. Xue","doi":"10.1117/12.741362","DOIUrl":"https://doi.org/10.1117/12.741362","url":null,"abstract":"We present a parallel Fourier domain optical coherence tomography (FD-OCT) system using Xenon flash lamp. Parallel configuration is implemented by one dimensional illumination and two dimensional detection with a CCD camera. The imaging axial resolution is 2.6μm in air with maximum depth range of ~650μm. The dynamic range is ~70DB with exposure time less than 1ms for a 1000*512 pixel image.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127892026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Response of diffuse reflectance light of two-layered tissue to optical properties of inner layer 双层组织漫反射光对内层光学特性的响应
International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741349
Dong-qing Peng, Hui Li, S. Xie
{"title":"Response of diffuse reflectance light of two-layered tissue to optical properties of inner layer","authors":"Dong-qing Peng, Hui Li, S. Xie","doi":"10.1117/12.741349","DOIUrl":"https://doi.org/10.1117/12.741349","url":null,"abstract":"Although measurement of diffuse reflectance light can be used to determine the optical properties of tissue noninvasively, great effort should be firstly devoted to the development of the relation between the reflectance and optical properties of tissue in our opinion. In this paper, light diffuse reflectance by a tissue optical model with two-layered structure is discussed by use of Monte Carlo method. The distribution of diffuse reflectance vs. emitting angle at different optical properties of inner layer is calculated. In addition, the influence of the changing absorption coefficient, scattering coefficient of the inner layer on the light diffuse reflectance from the surface is investigated and analyzed. The simulation results show that the diffuse reflectance light is sensitive greatly to micro-change of optical properties (absorption coefficient and scattering coefficient) of the inner layer which has small value of optical properties. We believe that the results will be useful for the development of determination the optical parameters of bio-tissue in vivo.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"6534 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129010701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A promising method based on surface plasmon resonance for quantitative analysis of biological samples 一种基于表面等离子体共振的生物样品定量分析方法
International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741483
Zhonghua Dong, Cheng Deng, Shukuan Xu, Jiang Zhu, Yang Yang, Xiaoyong Yang, Guoliang Huang
{"title":"A promising method based on surface plasmon resonance for quantitative analysis of biological samples","authors":"Zhonghua Dong, Cheng Deng, Shukuan Xu, Jiang Zhu, Yang Yang, Xiaoyong Yang, Guoliang Huang","doi":"10.1117/12.741483","DOIUrl":"https://doi.org/10.1117/12.741483","url":null,"abstract":"Surface plasmon resonance (SPR) technique is based on an optical measurement approach that is highly sensitive to the refractive index unit (RIU) of the sample on its analysis surface. Here, we demonstrate the direct detection of proteins and small molecules using an advanced SPR technology with a sensitivity that is as good as Fourier transform infrared (FTIR) spectroscopy. Some quantitative results are reported in this paper.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"121 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116835238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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