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International Conference on Photonics and Imaging in Biology and Medicine最新文献

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Analysis of microscopic mechanism on electricity and thermology effects of laser-plasmalemma interaction 激光-质膜相互作用电学和热学效应的微观机理分析
International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741368
Ling-yun Zhou, Can-bang Zhang, Sheng-Yu Wang, Lin Xu, Gang Wu
{"title":"Analysis of microscopic mechanism on electricity and thermology effects of laser-plasmalemma interaction","authors":"Ling-yun Zhou, Can-bang Zhang, Sheng-Yu Wang, Lin Xu, Gang Wu","doi":"10.1117/12.741368","DOIUrl":"https://doi.org/10.1117/12.741368","url":null,"abstract":"The microscopic mechanism of the electricity and thermology effects of interaction of laser with plasmalemma are researched by electromagnetics, quantum mechanics and quantum statistics. We derived the formulas on the polarigalion effects and \"temperatue-rising effect\" of laser-plasmalemma interaction. The results of our theory recherch can explain some experiments.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127846528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Gold nanoshells as solid-phase dot assay labels 金纳米壳作为固相点分析标签
International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741502
B. Khlebtsov, L. Dykman, V. Bogatyrev, N. Khlebtsov
{"title":"Gold nanoshells as solid-phase dot assay labels","authors":"B. Khlebtsov, L. Dykman, V. Bogatyrev, N. Khlebtsov","doi":"10.1117/12.741502","DOIUrl":"https://doi.org/10.1117/12.741502","url":null,"abstract":"We report on the first application of silica-gold nanoshells to a solid-phase dot immunoassay. The assay principle is based on staining of a drop (1μL ) analyte on a nitrocellulose membrane strip by using silica/gold nanoshells conjugated with biospecific probing molecules. Experimental example is human IgG (hIgG, target molecules) and protein A (probing molecules). For usual 15-nm colloidal gold conjugates, the minimal detectable amount of hIgG is about 4ng. By contrast, for nanoshell conjugates (silica core diameter of 70 nm and gold outer diameter of 100 nm) we have found significant increase in detection sensitivity and the minimal detectable amount of hIgG is about 0.5 ng.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125400098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Registration of intraoperative optical image sequence 术中光学图像序列的配准
International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741331
Ming Li, Yugang Jiang, Yadong Liu, Lingyun Zhang, Xiaogang Chen, D. Hu
{"title":"Registration of intraoperative optical image sequence","authors":"Ming Li, Yugang Jiang, Yadong Liu, Lingyun Zhang, Xiaogang Chen, D. Hu","doi":"10.1117/12.741331","DOIUrl":"https://doi.org/10.1117/12.741331","url":null,"abstract":"In neurosurgery, cortical warping is one of the significant sources of noises during optical imaging after the skull and the dura have been removed. The optical image sequence must be registered for further data analysis. The registration algorithms in widely used medical image tools, e.g. Automated Image Registration (AIR), Statistical Parametric Mapping (SPM), usually express the cortical warping as polynomials or terms of cosine basis. However, these nonlinear models do not faithfully fit the elastic warping of the cortexes, and thus can not achieve a satisfactory result. Based on the elastic model, i.e., the approximating thin-plate splines (aTPS), we propose herein an improved aTPS (iaTPS) algorithm to deal with the elasticity of the cortical warping. In the cost function of the original aTPS algorithm, landmarks with different localization uncertainties should be given different weights, however, due to the absence of a convincing method to specify these weights, a same weight value was manually set for all landmarks in practice. In our iaTPS algorithm, landmarks are categorized into several classes (usually 3~5) by their localization uncertainties, and the weights for each class are decided by an optimization process. The comparison experiment on the intraoperative optical data of human brain has shown that the new algorithm can offer better registration accuracy than the aTPS algorithm.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121564962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Restoration of fluorescence images from two-photon microscopy using modified nonlinear anisotropic diffusion filter 利用改进的非线性各向异性扩散滤波器恢复双光子显微镜荧光图像
International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741498
Hongmin Zhang, Qingming Luo, S. Zeng
{"title":"Restoration of fluorescence images from two-photon microscopy using modified nonlinear anisotropic diffusion filter","authors":"Hongmin Zhang, Qingming Luo, S. Zeng","doi":"10.1117/12.741498","DOIUrl":"https://doi.org/10.1117/12.741498","url":null,"abstract":"Two-photon laser scanning fluorescence microscopy is becoming a powerful tool in study of neuron functional imaging in vivo for its inherent deeper penetration, less photo-damage. Now, with the two-photon fluorescence images of brain tissue, we can reconstruct three-dimensional neuronal morphologies easily. However, the images usually are obscured by a lot of noise, in particular in deep tissue with strong excitation laser power. Therefore, good image restoration technique that could remove the noise while preserve neuronal structure is crucial for the results of subsequent image segmentation and neuron reconstruction. Here, we propose a modified nonlinear anisotropic diffusion filter which incorporates both gradient and gray-level variance of raw data, to remove the noise, rather than merely considers gradient as the classical Perona-Malik nonlinear anisotropic diffusion model. Experimental results have shown that the proposed scheme can remove noisy speckles effectively while maintain the shape of neuronal morphologies in two-photon fluorescence images without conflict.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116112043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Optimal sizes of gold nanoparticles for laser treatment of tumors 激光治疗肿瘤的最佳金纳米颗粒尺寸
International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741501
A. Popov, A. Priezzhev, R. Myllylä
{"title":"Optimal sizes of gold nanoparticles for laser treatment of tumors","authors":"A. Popov, A. Priezzhev, R. Myllylä","doi":"10.1117/12.741501","DOIUrl":"https://doi.org/10.1117/12.741501","url":null,"abstract":"In the present paper, the problem of laser treatment of tumors by means of gold nanoparticles is discussed. One of the mechanisms of cancerous cells destruction is formation of small bubbles on cell membranes and inside cells around gold nanoparticles induced by laser pulse absorption by the particles. It is shown by Mie theory calculations that the optimal spectral region for such tumor treatment is within 400 - 550 nm with the corresponding nanoparticles sizes to be 30 - 40 nm. The appropriate durations of laser pulses for such kind of laser therapy are under 1 ns in this spectral region.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"145 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131545870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
The analysis and practical application about the effect of intra-vascular laser irradiation on C-C bond 血管内激光照射对C-C键影响的分析及实际应用
International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741297
Can-bang Zhang, Zhi-Fu Dai, Li-Li Zhang, Jia Tian, Ling-yun Zhou
{"title":"The analysis and practical application about the effect of intra-vascular laser irradiation on C-C bond","authors":"Can-bang Zhang, Zhi-Fu Dai, Li-Li Zhang, Jia Tian, Ling-yun Zhou","doi":"10.1117/12.741297","DOIUrl":"https://doi.org/10.1117/12.741297","url":null,"abstract":"C-C bond is the most common kind of interactions in biological molecules. For example, the molecules of cholesterol and fibrinogen are connected with C-C bonds. We analysis the effect of Laser onto C-C bond in medical cure case by the method of Intra-vascular Laser Irradiation on blood (ILIB), and in theory a new model of \"Laser-(C-C)\" inharmonic oscillator is constructed on the base of quantum mechanics. Furthermore, we investigate the helpful influence of ILIB through sixty-two cases of brain-thrombus.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115152703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preliminary research of measurement methods for optical property parameters in fruit tissue 水果组织光学特性参数测量方法的初步研究
International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741475
Jianping Wen, Xing-miao Chen, Yande Liu
{"title":"Preliminary research of measurement methods for optical property parameters in fruit tissue","authors":"Jianping Wen, Xing-miao Chen, Yande Liu","doi":"10.1117/12.741475","DOIUrl":"https://doi.org/10.1117/12.741475","url":null,"abstract":"The research for optical transmission in biomedicine has been studied for many years at domestic and abroad, but the applicative research for nondestructive measuring in fruit internal qualities has not been found nowadays. Therefore, the transmission mechanism and the research methods of optical transmission in biomedicine can be applied in fruit internal qualities analysis. It will give a chance to improve measuring precision in fruit internal qualities. In this paper, the optical transmission mechanism in fruit tissue, the optical property parameters of fruit tissue, the models of optical transmission in fruit tissue and the measurement methods of optical parameters in fruit tissue have been researched respectively. Meanwhile, an important measurement method, namely integrating sphere technology, is presented. It will provide the theoretical foundation for the research for nondestructive measuring in fruit internal qualities.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133738781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Improvement of axial resolution in optical coherence tomography by optical pupil filter 光学瞳孔滤光片提高光学相干断层成像的轴向分辨率
International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741435
Lin Zhou, Z. Ding, J. Meng, Zhenming Zhou
{"title":"Improvement of axial resolution in optical coherence tomography by optical pupil filter","authors":"Lin Zhou, Z. Ding, J. Meng, Zhenming Zhou","doi":"10.1117/12.741435","DOIUrl":"https://doi.org/10.1117/12.741435","url":null,"abstract":"Axial resolution is a key factor in optical coherence tomography (OCT). Biomedical applications will benefit from improved resolution and quality that ultrahigh resolution OCT can provide. Existing approaches to improve axial resolution of OCT mostly depend on new broadband light sources, which are always costly and inconvenient in instrumentation. In this paper we adopt an alternative method to enhance the axial resolution of OCT by combining coherence gate with optical superresolution. A three-zone phase pupil filter is designed and inserted into the sample arm of OCT. The depth responses measured demonstrate that an improvement of more than 15% in axial resolution is achieved in the proposed OCT system.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132135127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Determination of optical properties in turbid medium based on time-resolved determination 基于时间分辨法的混浊介质光学特性测定
International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741361
D. Qin, Zheng Ma, F. Gao, Huijuan Zhao
{"title":"Determination of optical properties in turbid medium based on time-resolved determination","authors":"D. Qin, Zheng Ma, F. Gao, Huijuan Zhao","doi":"10.1117/12.741361","DOIUrl":"https://doi.org/10.1117/12.741361","url":null,"abstract":"In this article, we briefly described a time-correlated single photon counting (TCSPC) system specifically designed for extracting the optical properties in turbid medium. This system was evaluated by use of two sets of liquid tissue-simulating phantoms containing different concentrations of Intralipid-10% as scatters and India ink as absorbers. With the distribution of times of flight (DTOF) of photons measured by the TCSPC system, some featured parameters, such as the mean time of flight and the variance of DTOF were calculated. Based on these parameters, we developed a simple and fast method to obtain the absorption coefficient μa and reduced scattering coefficient μs of the turbid medium. Furthermore, the accuracy of the method was validated using the Monte Carlo simulations. It was found that the optical properties could be extracted with this method, which was much faster than the conventional curve-fitting procedure. Our method could be useful in on-line monitoring of optical properties of biological tissue.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134219336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Synchronies in cultured neuronal network 培养神经网络中的同步现象
International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741466
Yunsheng Lin, Lin Chen, S. Zeng, Qingming Luo
{"title":"Synchronies in cultured neuronal network","authors":"Yunsheng Lin, Lin Chen, S. Zeng, Qingming Luo","doi":"10.1117/12.741466","DOIUrl":"https://doi.org/10.1117/12.741466","url":null,"abstract":"The neuronal network cultured in virto used as an important tool for study have been realized by more and more researchers owing to its non-invasive nature. But till now, there isn't a parameter that can conveniently describes the changing states of neuronal network from the whole. In this paper, the synchrony calculation acted as the reactive results of the neuronal network to electrical stimulation (used for learning training) or bicuculine is analyzed and the variety of the synchrony of the network is tried as an important value to depict the diversification of the neuronal network. These experimental results processed in this way are given out in the end of this paper.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116227059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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