International Conference on Photonics and Imaging in Biology and Medicine最新文献_第10页

The mechanism and influencing factors on photodynamic purging of leukemia cells 白血病细胞光动力清除的机制及影响因素

International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741594

Zhongming Li, Zhen-xi Zhang

{"title":"The mechanism and influencing factors on photodynamic purging of leukemia cells","authors":"Zhongming Li, Zhen-xi Zhang","doi":"10.1117/12.741594","DOIUrl":"https://doi.org/10.1117/12.741594","url":null,"abstract":"The paper compared the response of HL60 cells with peripheral blood mononuclear cell (PBMC) after 5-ALA PDT for different periods of time and then followed by irradiating with different wavebands for different fluences. The results of parameter experiments were obtained: 1 × 105/mL HL60 cell was incubated with 1 mmol/L ALA in dark for 4 h and the maximum fluorescence of PpIX level appeared; then irradiated with 410 nm (4 mW/cm2) for 14.4 J/cm2 and maximum photodamage to membrane and mitochondrial function of HL60 cell resulted. Analyzed the experimental results of references, we got that the effect of photodynamic purging of leukemia cells may be modulated by the leukemia cell type; the photosensitizer's type, dose, and its location point; the incubation time and condition; the light wavelength and its dosage. If the light is delivered at a high rate, significant heating of a molecule and its surrounding may take place. A too low dosage has no effect at all or will result in high relapse rate due to the presence of cancer cells. The wavelength and intensity of light required to carry out photopurging is determined by the absorption properties of the photosensitizer. The light dose actually received by the photosensitizer and tissue may be higher or lower depending on the manner of irradiation.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122168238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A rapid gradient segmentation method for edge recognition of biomedical image 一种用于生物医学图像边缘识别的快速梯度分割方法

International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741438

Guannan Chen, ZhongJian Teng, Kuntao Yang, Rong Chen

引用次数: 0

Temporal and spatial characteristics of bid and bax translocation during UV-induced apoptosis 紫外光诱导细胞凋亡过程中bid和bax易位的时空特征

International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741588

Yinyuan Wu, D. Xing, Lei Liu, Tongsheng Chen

{"title":"Temporal and spatial characteristics of bid and bax translocation during UV-induced apoptosis","authors":"Yinyuan Wu, D. Xing, Lei Liu, Tongsheng Chen","doi":"10.1117/12.741588","DOIUrl":"https://doi.org/10.1117/12.741588","url":null,"abstract":"UV irradiation is a DNA-damage agent that triggers apoptosis through both the membrane death receptor and the mitochondrial apoptotic signaling pathways. Bid and Bax are two important proapoptotic members of the Bcl-2 family, localize largely in the cytoplasm and redistribute to mitochondria in response to most apoptotic stimuli. Cells deficient in Bax are resistant to UV-induced apoptosis, cells deficient in Bid are less susceptible than normal cells in response to DNA damage. Thus, studying characteristics of Bid and Bax translocation by UV irradiation is very important for us to understand the cellular signaling mechanisms mediating UV-induced apoptosis. In this study, to investigate Bid and Bax translocation in real time in a single cell by UV irradiation, we transfected Bid-CFP, YFP-Bax and DsRed-Mit into human lung adenocarcinoma cells (ASTC-a-1), then observed temporal and spatial characteristics of Bid and Bax translocation by laser confocal scanning microscope imaging technique. Our results showed that Bax translocation was earlier than Bid translocation and the average duration of Bax translocation was about 20-30 min during UV-induced apoptosis.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128774746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nondestructive determination of pear internal quality indices by near-infrared spectrometry 近红外光谱法无损测定梨内部品质指标

International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741474

Yande Liu, Xing-miao Chen, Aiguo Ouyang, Y. Ying

{"title":"Nondestructive determination of pear internal quality indices by near-infrared spectrometry","authors":"Yande Liu, Xing-miao Chen, Aiguo Ouyang, Y. Ying","doi":"10.1117/12.741474","DOIUrl":"https://doi.org/10.1117/12.741474","url":null,"abstract":"The objectives of the study were to establish relationships between the nondestructive near-infrared (NIR) spectral measurements and the major internal quality indices of pear ('Fengshui', Jiangxi) fruit, and to evaluate the use of NIR spectrometry in measuring the internal quality indices of pear fruit. Intact pear fruit were measured by reflectance NIR in 350-1800 nm range. In this study, Calibration models relating NIR spectra to soluble solids content (SSC), and firmness were developed based on multi-linear regression (MLR), Principal component analysis (PCA) and partial least square (PLS) regression with respect to the logarithms of the reflectance reciprocal log (1/R), its first derivative D1log (1/R)and second derivative D2log (1/R). The best combination, based on the prediction results, was MLR models with respect to D1log (1/R) at equatorial position of pear fruit. Prediction with MLR models resulted correlation coefficients (Rp) of 0.9151 and 0.8125, and root mean standard error of prediction (RMSEP) of 0.6834 and 1.3778 for SSC and firmness, respectively. The preliminary results of the built models indicated that NIR spectroscopy could provide an accurate, reliable and nondestructive method for assessing the internal quality indices of pear fruit.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"52 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120863973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound-modulated optical phenomena in scattering media driven by a pulsed transducer 由脉冲换能器驱动的散射介质中的超声调制光学现象

International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741366

Jiali Cai, Li-li Zhu, Cuncheng Weng, Hui Li

引用次数: 1

A novel analysis method for near infrared spectroscopy based on Hilbert-Huang transform 基于Hilbert-Huang变换的近红外光谱分析新方法

International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741479

Zhenyu Zhou, Hongyu Yang, Yun Liu, Zongcai Ruan, Qingming Luo, H. Gong, Zuhong Lu

{"title":"A novel analysis method for near infrared spectroscopy based on Hilbert-Huang transform","authors":"Zhenyu Zhou, Hongyu Yang, Yun Liu, Zongcai Ruan, Qingming Luo, H. Gong, Zuhong Lu","doi":"10.1117/12.741479","DOIUrl":"https://doi.org/10.1117/12.741479","url":null,"abstract":"Near Infrared Imager (NIRI) has been widely used to access the brain functional activity non-invasively. We use a portable, multi-channel and continuous-wave NIR topography instrument to measure the concentration changes of each hemoglobin species and map cerebral cortex functional activation. By extracting some essential features from the BOLD signals, optical tomography is able to be a new way of neuropsychological studies. Fourier spectral analysis provides a common framework for examining the distribution of global energy in the frequency domain. However, this method assumes that the signal should be stationary, which limits its application in non-stationary system. The hemoglobin species concentration changes are of such kind. In this work we develop a new signal processing method using Hilbert-Huang transform to perform spectral analysis of the functional NIRI signals. Compared with wavelet based multi-resolution analysis (MRA), we demonstrated the extraction of task related signal for observation of activation in the prefrontal cortex (PFC) in vision stimulation experiment. This method provides a new analysis tool for functional NIRI signals. Our experimental results show that the proposed approach provides the unique method for reconstructing target signal without losing original information and enables us to understand the episode of functional NIRI more precisely.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"86 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131239628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single tapered fiber optical tweezers 单锥形光纤镊子

International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741478

Zhihai Liu, C. Guo, Jun Yang, Libo Yuan

引用次数: 1

Enhancement of taxol-induced apoptosis by inhibition of NF-κB with ursorlic acid 熊果酸抑制NF-κB增强紫杉醇诱导的细胞凋亡

International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741583

Yunlong Li, D. Xing

{"title":"Enhancement of taxol-induced apoptosis by inhibition of NF-κB with ursorlic acid","authors":"Yunlong Li, D. Xing","doi":"10.1117/12.741583","DOIUrl":"https://doi.org/10.1117/12.741583","url":null,"abstract":"Taxol is known to inhibit cell growth and triggers significant apoptosis in various cancer cells, and activation of proliferation factor NF-κB during Taxol-induced apoptosis is regarded as a main reason resulting in tumor cells resistance to Taxol. It has been found that ursorlic acid can inhibit the activation of NF-κB. In order to study whether ursorlic acid can enhance the Taxol-induced apoptosis, we use fluorescence resonance energy transfer (FRET) technique and probe SCAT3 to compare the difference of caspase-3 activation between Taxol alone and Taxol combined ursorlic acid. With laser scanning confocal microscopy, we find that ursorlic acid, a nontoxic food component, sensitizes ASTC-a-1 cells more efficiently to Taxol-induced apoptosis by advanced activation of caspase 3. The result also suggests that there would be a synergistic effect between Taxol and ursorlic acid, and the more detailed mechanism of synergistic effect needs to be clarified further, such as the correlations among NF-κB, Akt, caspase 8, which leads to the advanced activation of caspase 3 during combined treatment of Taxol and ursorlic acid. Moreover, this may be a new way to improve Taxol-dependent tumor therapy.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132579995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

80-W green KTP laser used in photoselective laser vaporization of the prostrate by frequency doubling of Yb3+ -doped large-mode area fiber laser 80w绿色KTP激光器用于掺Yb3+的大模区光纤激光器的倍频光选择性激光汽化

International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741617

Hongxing Xia, Zhengjia Li

引用次数: 1

A novel method for rapid and non-invasive detection of plants senescence using delayed fluorescence technique 一种利用延迟荧光技术快速、无创检测植物衰老的新方法

International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741637

Lingrui Zhang, D. Xing, Junsheng Wang, Lizhang Zeng, Qiang Li

{"title":"A novel method for rapid and non-invasive detection of plants senescence using delayed fluorescence technique","authors":"Lingrui Zhang, D. Xing, Junsheng Wang, Lizhang Zeng, Qiang Li","doi":"10.1117/12.741637","DOIUrl":"https://doi.org/10.1117/12.741637","url":null,"abstract":"Plants senescence is a phase of plants ontogeny marked by declining photosynthetic activity that is paralleled by a decline in chloroplast function. The photosystem II ( PSII ) in a plant is considered the primary site where light-induced delayed fluorescence (DF) is produced. With the leaves of Catharanthus roseus (Catharanthus roseus (L.) G.Don) as testing models, we have studied the effects of plants senescence induced by dark and/or exogenous hormones treatments on characteristics of DF by using a home-made portable DF detection system, which can enable various DF parameters, such as DF decay kinetic curve and DF intensity, to be rapidly produced for the plants in a short time. The results show that the changes in DF intensity of green plants can truly reflect the changes in photosynthetic capacity and chlorophyll content. Therefore, DF may be used an important means of evaluating in vivo plants senescence physiology. The changes in DF intensity may provide a new approach for the rapid and early detection of plants senescence caused by age or other senescence-related factors. DF technique could be potential useful for high throughput screening and less time-consuming and automated identifying the interesting mutants with genetic modifications that change plants senescence progress.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133443766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2