Dental Research Journal最新文献

{"title":"The influence of internally architected voids in the creation of high-strength, low-weight 3D-printed cobalt-chromium prototypes","authors":"Abdullah Jasim Mohammed, Ahmed Asim Al-Ali","doi":"10.4103/drj.drj_627_23","DOIUrl":"https://doi.org/10.4103/drj.drj_627_23","url":null,"abstract":"\u0000 \u0000 The additive manufacturing technology made the topology optimization technique feasible. This technique can indefinitely reduce the weight of the printed items with a promising increase in the mechanical properties of that item.\u0000 \u0000 \u0000 \u0000 In the current experimental study, 50 samples were fabricated for a 3-point bending test. They were divided into ( n = 5) as a control Group 1 free of internal geometries, ( n = 15) for each of Groups 2-4, and they were subdivided into ( n = 5) for each percentage of reduction per volume (10%, 15%, and 20%). Spherical, ovoid, and diamond shapes were each group's fundamental geometries, respectively. Cylindrical tunnels connected the voids in each group. Radiographic images were performed to validate the created geometries, the weight was measured, and flexural strength and modulus of elasticity were calculated. Data were analyzed by one-way ANOVA and Duncan's post hoc tests at P <s 0.05.\u0000 \u0000 \u0000 \u0000 The weight results showed a significant reduction in mass. The flexural strength of Group 2 at a 10% reduction per volume had the highest mean significantly without compromising the elastic modulus. In comparison, the means of group 4 at 20% reduction showed the lowest level of toughness.\u0000 \u0000 \u0000 \u0000 The weight was reduced according to the reduction percentage. The flexural strength of Group 2 at a 10% reduction showed the highest degree of toughness among all groups. The void shape and density influenced the mechanical properties tested.\u0000","PeriodicalId":11016,"journal":{"name":"Dental Research Journal","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140404396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardization of honey as a tissue fixative for histopathology: A morphometric study","authors":"B. Sabarinath, Shruthi Suresh, G. Protyusha, S. Dhanarathna","doi":"10.4103/drj.drj_88_23","DOIUrl":"https://doi.org/10.4103/drj.drj_88_23","url":null,"abstract":"\u0000 \u0000 Tissue fixation is a crucial step to preserve the tissues in a life-like state with minimal disruption to its cellular and chemical composition for histopathological examination. The search for an effective alternate tissue fixative to the routinely used formaldehyde has gained interest as constant exposure to formaldehyde has proven to be toxic. Honey, an organic substance with high acidity and hygroscopic nature, exhibits tissue fixative properties and has been used in the present study. The present study aimed to standardize honey as a tissue fixative for histopathology by comparing it with formalin.\u0000 \u0000 \u0000 \u0000 \u0000 In vitro study Oral tissue samples of goat were fixed in 10% honey and 10% formalin solution, respectively, for 24-48 h, followed by routine tissue processing and microscopic examination of 37 slides per group. 2200 epithelial cells (1100 per group) were selected for the computer-aided morphometric image analysis (Fiji-Image J) by three observers. Cell area (CA), cell perimeter (CP), nuclear area (NA), nuclear perimeter (NP), cytoplasmic area (Cyt A), and nuclear-cytoplasmic ratio were the parameters studied. Mann-Whitney U-test (STATA/IC version 16) for inter-group comparison was done and P < 0.05 was considered statistically significant.\u0000 \u0000 \u0000 \u0000 The probability of epithelial cells in the honey-fixed group to have greater NA, NP, and N/C ratio was about 50%-60%. The probability of epithelial cells in formalin-fixed tissues to have greater CA, CP, and Cyt A was about 70%.\u0000 \u0000 \u0000 \u0000 Honey is a better nuclear fixative than formalin. Cytoplasmic shrinkage of epithelial cells should be taken into consideration while fixing tissues with honey.\u0000","PeriodicalId":11016,"journal":{"name":"Dental Research Journal","volume":"26 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140407664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison between three methods of diode laser 810 nm, photodynamic therapy with laser 660 nm, and hypochlorite solution for disinfection of pulp canal of primary teeth","authors":"Nima Amini Tehrani, S. Javadinejad, A. Shirani","doi":"10.4103/drj.drj_82_23","DOIUrl":"https://doi.org/10.4103/drj.drj_82_23","url":null,"abstract":"\u0000 \u0000 The presence of treatment-resistant microorganisms is known as the main cause of pulpectomy failure in the endodontic treatment of deciduous teeth. The usage of lasers can contribute to reducing these microorganisms. This study aimed to compare the effect of three disinfection methods for deciduous teeth canals using laser diode 810 nm, photodynamic therapy with laser 660 nm and methylene blue, and sodium hypochlorite.\u0000 \u0000 \u0000 \u0000 In this experimental study, 58 single-root deciduous teeth with no root resorption were investigated in four groups, including one control group of 10 and three intervention groups of 16. Preparation of the samples was done using manual files up to three numbers after the initial file. After sterilizing the samples in an autoclave, Enterococcus faecalis bacteria were cultured in the canals. In the first group, irrigating with hypochlorite 2.5% was done; in the second group, photodynamic therapy was performed using a laser diode and 0.1 mg/mL methylene solution; and in the third group, high-intensity laser 810 nm direct radiation was done into the canal. Next, samples were taken from all canals. The colony formation unit (CFU) of the bacteria was counted in the blood agar culture medium. The data were analyzed using Kruskal-Wallis and negative binomial regression test (α =0.05).\u0000 \u0000 \u0000 \u0000 The mean CFU differed significantly between the four groups. The rate of incidence of E. faecalis colonies showed a reduction in all three intervention groups compared to the negative control. In the high-intensity laser 810 nm group, there was 68.4%; in the photodynamic therapy with diode 660 nm and methylene blue, there was 88%; and in the hypochlorite group, 98.3% reduction was observed compared to the negative control group.\u0000 \u0000 \u0000 \u0000 Based on the results of this study, to compare three disinfection methods of the deciduous teeth canals without preparation of canals, sodium hypochlorite had greater efficiency. All three groups of laser, photodynamic therapy, and sodium hypochlorite showed reductions of E. faecalis bacterial colony compared to the control group. The reductive effects of CFU were greater in the hypochlorite sodium group, followed by photodynamic and direct laser radiation groups.\u0000","PeriodicalId":11016,"journal":{"name":"Dental Research Journal","volume":"306 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140402343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of taste score and fungiform papillae quantification using digital image analysis in COVID-19 patients with smell and taste dysfunction.","authors":"Vidya Chikkarahalli Srikantaiah, Anil Somashekara Bilimale, Vidya Gowdappa Doddawad, Srinath Kenkere Marulaiah, Hathur Basavana Gowdappa, Ranjitha Shankaregowda, Basavegowda Madhu, Amulya Manohar Thotambailu, Raghu Ram Achar","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>The COVID-19 pandemic which is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has created a major global health crisis in recent years. Despite this, there have been few studies that have utilized reliable methods to assess changes in taste and smell perception. Therefore, our study aims at the number of fungiform papillae and objective measures of taste perception relationship among COVID-19 patients with olfactory and gustatory disorders.</p><p><strong>Materials and methods: </strong>This was a cross-sectional analytical study in which 57 COVID-19 patients were recruited who confirmed the dysfunction of taste and smell. Objective assessment of the sense of taste was evaluated using four different standardized solution preparations, and the scores were given according to the patient's statements. Digitalized quantification of fungiform papillae was counted. The data were analyzed with the Pearson's correlation coefficient using the SPSS version. 23 [Licensed JSSAHER, Mysuru, Karnataka, India], and the level of significance was set at <0.001.</p><p><strong>Results: </strong>In terms of altered or reduced taste and smell, male patients exhibited a higher incidence compared to females. Compared to the sour taste, a substantial number of COVID-19 patients have displayed a notable decrease in their ability to taste sweet, salty, and bitter flavors. However, a statistically significant positive correlation was observed between taste scores and fungiform papillae density (<i>r</i> = 0.518, <i>P</i> < 0.001).</p><p><strong>Conclusion: </strong>Our Study demonstrated that the quantitative evaluation of taste perception and the count of fungiform papillae can serve as important indicators of SARS-CoV-2 infection, and could potentially help in the early detection and treatment of COVID-19 patients, as reduced taste function is a significant marker of the disease.</p>","PeriodicalId":11016,"journal":{"name":"Dental Research Journal","volume":"21 ","pages":"11"},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10929720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140109544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphometric parameters of dental pulp in immature teeth in a sheep model after mechanical pulp exposure and restoration with reinforced zinc oxide-eugenol.","authors":"Eshaghali Saberi, Zahra Heidari, Hamidreza Mahmoudzadeh-Sagheb, Mahdieh Narouei, Lida Jafari, Alireza Mahmoudzadeh-Sagheb, Mahmoud Saadatian","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>The aim of the study was to investigate the morphometric parameters of dental pulp in open apices immature teeth in a sheep model after mechanical pulp exposure and restoration with reinforced zinc oxide-eugenol.</p><p><strong>Materials and methods: </strong>In this experimental study, a total of 12 immature mandibular central incisors from six adult male sheep, weighing 30-40 kg and with the age of 1 year old with Merino race were examined. After anesthesia, the pulps of the teeth in the case group were mechanically exposed and then were restored with reinforced zinc oxide-eugenol and amalgam. In the control group, the teeth remained intact. The animals were sacrificed at intervals of 2, 4, 6, and 8 weeks (E2, E4, E6, and E8) in the case and 2 and 8 weeks (C2 and C8) in the control groups. Then, their teeth were removed with the surrounding supporting tissues and alveolar bones. Tissue processing and staining were done, and the sections were examined under a light microscope. The Kruskal-Wallis and Mann-Whitney <i>U</i> tests were used to analyze the data and compare the changes between the two groups. <i>P</i> < 0.05 was considered statistically significant.</p><p><strong>Results: </strong>In response to mechanical exposure, reparative or tertiary dentin was formed, and its thickness increased during the time of the study. The thickness of the odontoblastic layer in the E4 group was the highest amount. The pulp chamber diameter in the C2 group was significantly larger than the other groups, and the diameter of the apical foramen in the E8 was decreased significantly compared to the controls (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>In response to mechanical exposure and restoration with reinforced zinc oxide-eugenol, some morphometric parameters of the dental pulp changed significantly in the sheep model compared to the controls.</p>","PeriodicalId":11016,"journal":{"name":"Dental Research Journal","volume":"21 ","pages":"17"},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10929729/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140109545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between periodontal disease and obstructive sleep apnea in adults: A systematic review.","authors":"Edoardo Bianchi, Marzia Segù, Andrea Toffoli, Geremia Razzini, Guido Maria Macaluso, Edoardo Manfredi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The purpose of this systematic review was to evaluate whether there are scientific evidence regarding the association between periodontitis and obstructive sleep apnea (OSA) in adults. An electronic search was performed on MEDLINE/PubMed for prospective and retrospective longitudinal studies, cohort studies, and case-control studies conducted in human adults affected by both OSA and periodontitis. Two reviewers extracted the data using a custom Excel spreadsheet. A methodological assessment of the quality of the studies was performed using the Newcastle-Ottawa Scale. Fourteen studies were included. All studies evaluated the association between periodontitis and OSA. None of the studies evaluated the cause-effect relationship. Eleven studies found a significant positive relationship between periodontitis and OSA, whereas three found no statistically significant association. Several study limitations were observed, such as lack of standardization of study groups, diagnosis of periodontitis and OSA, and differences in study design. Evidence of a plausible association between periodontitis and OSA was found. The possible relationship could be explained by systemic inflammation, oral breathing, and the comorbid relationship attributable to common risk factors. Observational and randomized controlled studies are needed to clarify the mechanism of interaction between the two conditions.</p>","PeriodicalId":11016,"journal":{"name":"Dental Research Journal","volume":"21 ","pages":"15"},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10929726/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140109546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of apical transportation and centering ability of three single-file systems in severely curved canals using micro-computed tomography.","authors":"Seyed Mohsen Hasheminia, Samaneh Soltani, Fateme Mohamadian, Masoud Saatchi, Seyed Mohsen Sadeghi","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to compare the apical transportation and centering ability of One Curve, HyFlex EDM, and EdgeFile X1 in curved mesiobuccal and mesiolingual canals of mandibular first molars.</p><p><strong>Materials and methods: </strong>In this <i>in vitro</i> experimental study, 60 mesiobuccal and mesiolingual canals of the mandibular first molars with a minimum length of 19 mm and 25°-40° curvature were randomly divided into three groups (<i>n</i> = 20) for root canal preparation with One Curve, HyFlex EDM, and EdgeFile X1. After access cavity preparation and confirming the glide path, the baseline micro-computed tomography (micro-CT) scans were obtained, and the root canals were instrumented with the respective systems according to the manufacturers' instructions. Apical transportation and centering ability were assessed at 1, 3, 5, and 7 mm from the apex by comparing pre- and postinstrumentation micro-CT scans. One-way ANOVA, independent <i>t</i>-test, and Duncan's <i>post hoc</i> test were used to statistically compare the groups, and data were analyzed by SPSS version 24 (alpha = 0.05).</p><p><strong>Results: </strong>The three groups were not significantly different regarding apical transportation at 5 and 7 mm from the apex (<i>P</i> > 0.05). At 1 mm level, One Curve caused significantly lower apical transportation; while, at 3 mm level, HyFlex EDM resulted in significantly higher apical transportation (<i>P</i> < 0.05). No significant difference was noted in the centering ability of the three groups at 1, 3, and 5 mm from the apex (<i>P</i> > 0.05). At 7 mm level, EdgeFile X1 showed significantly lower centering ability (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>One Curve caused lower canal transportation in the apical third compared with EdgeFile and HyFlex EDM, but no significant difference was noted among the three in the coronal third of the roots.</p>","PeriodicalId":11016,"journal":{"name":"Dental Research Journal","volume":"21 ","pages":"12"},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10929722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140109543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-cancer effects of green synthesized gold nanoparticles using leaf extract of <i>Annona muricata</i>. L against squamous cell carcinoma cell line 15 through apoptotic pathway.","authors":"S Jeslin Mary, Veeran Veeravarmal, Prasanth Thankappan, Paramasivam Arumugam, Percy Ida Augustine, R Franklin","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>Oral cancer remains one of the most dreadful diseases in developing nations. Currently, there has been a rise in the prevalence of tongue squamous cell carcinoma (SCC), with a poor prognosis. The use of standard treatment approaches against oral cancer patients brings about several side effects. In recent years, nanomedicine has provided a versatile platform for developing new targeted therapeutic modalities. However, safety remains a concern in the synthesis of nanoparticles (NPs). Therefore, the present study aims to synthesize safer phytoconstituent-mediated gold NPs (AuNPs) utilizing leaf extracts of <i>Annona</i> <i>muricata</i>, where the biochemical components of the plant leaf act as the reducing and capping agents in the synthesis of NPs, and to evaluate its anti-cancer activity against SCC.</p><p><strong>Materials and methods: </strong>In this <i>in vitro</i> experimental study, AuNPs were synthesized through an effective, simple, and ecologically sound green synthesis method. After characterization of these synthesized AuNPs, <i>in vitro</i> assays such as 3-(4, 5-dimethylthiazole2-yl)-2, 5-biphenyl tetrazolium bromide, wound healing, and clonogenic assays were carried out to investigate the anti-cancer potential of green synthesized AuNPs in the human tongue SCC cell line (SCC-15), and the possible mechanism of action was evaluated through gene and protein expression analysis of Bax, Bcl-2, and p53 genes. The results were expressed as mean ± standard deviation using Statistical Package for Social Sciences (SPSS) 20.0 software and Student's <i>t</i>-test was performed for experimental data. <i>P</i> ≤0.05 were considered statistically significant.</p><p><strong>Results: </strong>The <i>in vitro</i> assays demonstrated that the synthesized AuNPs are exhibiting anti-cancer activity by apoptosis of SCC-15 cells in a dose-dependent manner. Further, it also revealed a highly significant decrease in anti-apoptotic Bcl-2 gene expression, whereas pro-apoptotic genes p53 and Bax revealed a highly significant increase, which is statistically significant compared to the control (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>Our findings demonstrated that the AuNPs synthesized from <i>A.</i> <i>muricata</i> leaf extract could act as a novel anticancer agent, particularly against SCC, after further scrutiny.</p>","PeriodicalId":11016,"journal":{"name":"Dental Research Journal","volume":"21 ","pages":"14"},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10929727/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140109599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of coronal sealing of flowable composite, resin-modified glass ionomer, and mineral trioxide aggregate in endodontically treated teeth: An <i>in-vitro</i> study.","authors":"Mahla Tavakoli, Solmaz Araghi, Amirhossein Fathi, Shahriar Jalalian","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>Coronal seal is one of the essential factors that affects the success of endodontic treatment and reinforces the apical seal. The intra-orifice barrier is an efficient alternative approach to decrease coronal leakage in endodontically treated teeth and various materials have been used for this purpose. This study aimed to compare the coronal sealing of flowable composite, resin-modified glass ionomer (RMGI), and mineral trioxide aggregate (MTA) in endodontically treated teeth.</p><p><strong>Materials and methods: </strong>In this <i>in vitro</i> study, 35 single-canal canine teeth were divided into five groups, including flowable composite, RMGI, MTA, positive control, and negative control groups. The teeth were filled with restorative materials according to the factory's instructions. Afterward, the samples were immersed in 2% methylene blue dye solution for 1 week at 37°C and 100% humidity condition. Finally, the teeth were sectioned longitudinally and dye penetration was measured using a stereomicroscope with ×10. Data were analyzed with Kolmogorov-Smirnov and Kruskal-Wallis tests (α = 0.05).</p><p><strong>Results: </strong>The positive control group showed the highest amount of dye penetration compared to other groups (12.34 ± 0.46). Dye penetration in the MTA group was significantly lower (4.25 ± 0.31) compared to the RMGI group (5.94 ± 0.24) (<i>P</i> = 0.02). Moreover, while the dye penetration in the MTA group was lower than in the flowable composite group (5.65 ± 0.26), the difference was not statistically significant (<i>P</i> = 0.12).</p><p><strong>Conclusion: </strong>MTA reduces the coronal leakage and provides an acceptable coronal seal in endodontically treated teeth, especially compared to RMGI, and therefore, using MTA as an intra-orifice barrier increases the endodontic treatment success rate.</p>","PeriodicalId":11016,"journal":{"name":"Dental Research Journal","volume":"21 ","pages":"13"},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10929723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140109601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of antimicrobial efficacy of triple antibiotic paste and amoxicillin clavulanate paste as an intracanal medicament against <i>Enterococcus faecalis</i>: An <i>in vitro</i> study.","authors":"Dhandayuthapani Sasikala, Parisa Norouzi Baghkomeh, Jamaluddin Mohammed Farzan","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Background: </strong>Triple antibiotic paste (TAP) is the commonly used intracanal medicament against <i>Enterococcus</i> <i>faecalis</i>. Amoxicillin clavulanate paste (ACP) is recommended as a \"fall-back\" antibiotic when traditional dental antibiotics fail. Literature comparing the antimicrobial efficacy of TAP and ACP in eradicating <i>E.</i> <i>faecalis</i> from the root canal system is sparse; hence, this <i>in vitro</i> study was conducted to evaluate and compare the antimicrobial efficacy of TAP and ACP as an intracanal medicament for endodontic treatment of single-rooted permanent teeth against <i>E.</i> <i>faecalis.</i></p><p><strong>Materials and methods: </strong>This <i>in</i> <i>vitro</i>, experimental study evaluated 60 root samples obtained from extracted single-rooted human permanent teeth. The canal diameter was enlarged and subsequently infected with <i>E.</i> <i>faecalis</i> for 21 days. Four groups of the contaminated samples were treated with TAP, ACP, calcium hydroxide (positive control), and saline (negative control), respectively. Dentinal shavings were collected at the end of the 1^st, 7^th, and 10^th day and inoculated in agar plates. The number of colony-forming units was determined, and the data were statistically analyzed using the Kolmogorov-Smirnov and Shapiro-Wilks test. <i>P</i> <0.05 was considered statistically significant.</p><p><strong>Results: </strong>The mean number of <i>E.</i> <i>faecalis</i> colony counts across all 3 test days demonstrated that TAP exhibited the highest inhibition of bacterial growth, followed by ACP which is not statistically significant (<i>P</i> = 1.00).</p><p><strong>Conclusion: </strong>Considering the limitations of this <i>in vitro</i> study, the findings suggest that ACP could be an effective alternative intracanal medicament to TAP for endodontic therapy.</p>","PeriodicalId":11016,"journal":{"name":"Dental Research Journal","volume":"21 ","pages":"10"},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10929719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140109600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}