Current opinion in microbiology最新文献_第3页

Strategies and mechanisms of contact-dependent predation in bacteria 细菌接触依赖性捕食的策略和机制

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-07-25 DOI: 10.1016/j.mib.2025.102639

Coralie Tesseur , Yoann G Santin , Géraldine Laloux

{"title":"Strategies and mechanisms of contact-dependent predation in bacteria","authors":"Coralie Tesseur , Yoann G Santin , Géraldine Laloux","doi":"10.1016/j.mib.2025.102639","DOIUrl":"10.1016/j.mib.2025.102639","url":null,"abstract":"<div><div>Predatory bacteria kill other bacteria to acquire nutrients. Among diverse strategies to kill and feed on prey, contact-dependent predation stands out as it requires direct physical interaction between predator and prey. By delving into the mechanisms of contact-dependent predation among bacteria, this review illustrates the breadth of molecular and cellular determinants underlying these remarkable antagonistic interactions. We highlight structural, genetic, and imaging evidence supporting the roles of envelope protein machineries — pili and secretion systems — as well as adhesins and specialised membrane junctions, in mediating key steps of contact-dependent predation. Although detailed mechanistic understanding remains scarce, recurring themes reveal the repurposing of molecular devices that may undergo precise regulation to achieve predatory actions at the right place and time. Future research should identify the molecular nature of prey–predator interfaces and uncover how prey contact and consumption integrate with other cellular processes, potentially revealing novel bacterial cell cycle control pathways.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"87 ","pages":"Article 102639"},"PeriodicalIF":5.9,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthetic microbial communities for studying and engineering the tree microbiome: challenges and opportunities 用于研究和设计树木微生物组的合成微生物群落：挑战和机遇

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-07-22 DOI: 10.1016/j.mib.2025.102636

Marine C Cambon , Xinming Xu , Ákos T Kovács , Sofia IF Gomes , James E McDonald

{"title":"Synthetic microbial communities for studying and engineering the tree microbiome: challenges and opportunities","authors":"Marine C Cambon , Xinming Xu , Ákos T Kovács , Sofia IF Gomes , James E McDonald","doi":"10.1016/j.mib.2025.102636","DOIUrl":"10.1016/j.mib.2025.102636","url":null,"abstract":"<div><div>Trees are essential to ecosystems in both natural and urban environments, yet they are increasingly threatened by abiotic and biotic stresses linked to climate change and human activities. The use of microbial-based approaches and microbiome engineering to safeguard plants and crop production is promising, but their application in trees raises specific challenges. Here, we review knowledge on the tree microbiome and outline opportunities to leverage tree-associated microbial communities. We describe the specific challenges inherent to working with tree species and highlight how Synthetic Microbial Communities (SynComs) can be used to study and engineer tree microbiomes. Finally, we propose that future research priorities include (1) developing model tree systems for experimental work, (2) obtaining tree-specific culture collections and SynComs, and (3) optimising methods for tree SynCom inoculation. Unlocking these methodological challenges will enable us to realise the potential of the tree microbiome and address global challenges in tree health and the provision of ecosystem services.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"87 ","pages":"Article 102636"},"PeriodicalIF":5.9,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal organization of the bacterial cytoplasm 细菌细胞质的时空组织

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-07-17 DOI: 10.1016/j.mib.2025.102635

Dmitrii Linnik, Bert Poolman

引用次数: 0

Recent advances in natural and synthetic phosphonate therapeutics 天然和合成膦酸盐疗法的最新进展

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-07-12 DOI: 10.1016/j.mib.2025.102630

Jerry Cui , Kou-San Ju

引用次数: 0

Seeing between the lines: matrix-assisted laser desorption/ionization mass spectrometry imaging advances for microbial interactions 看字里行间：基质辅助激光解吸/电离质谱成像微生物相互作用的进展

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-07-12 DOI: 10.1016/j.mib.2025.102634

Laura Rodriguez-Velandia , Ethan A Older , Laura M Sanchez

{"title":"Seeing between the lines: matrix-assisted laser desorption/ionization mass spectrometry imaging advances for microbial interactions","authors":"Laura Rodriguez-Velandia , Ethan A Older , Laura M Sanchez","doi":"10.1016/j.mib.2025.102634","DOIUrl":"10.1016/j.mib.2025.102634","url":null,"abstract":"<div><div>This review delves into the technical advancements of matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) applications from the last 15 years, highlighting its importance as an analytical tool for exploring the complex interactions within microbial communities. Recognizing the limitations of standard laboratory cultivation practices, we examine how recent research has worked toward bridging the gap between controlled laboratory experiments and <em>in situ</em> measurements. We discuss the shift from traditional agar-based co-culture to newer methods, such as 3D MALDI-MSI and artificial environments, ultimately progressing toward <em>in situ</em> analysis. We highlight the innovative aspects of these methodologies, emphasizing the technical challenges of sample preparation and subsequent metabolite identification. While MALDI-MSI has proven to be an invaluable tool for mapping chemical communication within complex microbial communities, we acknowledge the significant bottlenecks that remain. We call on the researchers to use establish reporting standards and support the expansion of metabolomic databases for future discoveries.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"87 ","pages":"Article 102634"},"PeriodicalIF":5.9,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of nucleoid-associated proteins in mediating responses to environmental changes 核相关蛋白在介导环境变化反应中的作用

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-07-09 DOI: 10.1016/j.mib.2025.102628

Pingzhuang Ge , Fatema-Zahra M Rashid , Remus T Dame

引用次数: 0

Swimming or sessile: the interplay between c-di-GMP signalling and flagellar motility 游动或无根：c-二- gmp信号与鞭毛运动的相互作用

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-07-08 DOI: 10.1016/j.mib.2025.102632

Xuanlin Chen, Liyun Wang, Victor Sourjik

{"title":"Swimming or sessile: the interplay between c-di-GMP signalling and flagellar motility","authors":"Xuanlin Chen, Liyun Wang, Victor Sourjik","doi":"10.1016/j.mib.2025.102632","DOIUrl":"10.1016/j.mib.2025.102632","url":null,"abstract":"<div><div>Bacteria have evolved multiple strategies to thrive in diverse environments. These include the ability to make rapid transitions between motile and sessile lifestyles, either of which might be favoured dependent of the environmental conditions. The central regulator for these lifestyle transitions is the second messenger cyclic dimeric guanosine monophosphate (c-di-GMP), which in general, inhibits flagellar motility and promotes the formation of sessile biofilm communities. Reciprocally, flagellated cells suppress c-di-GMP synthesis or activate its degradation to preserve motility. The interplay between c-di-GMP signalling and motility occurs at multiple levels of regulation, with evolutionarily conserved general principles but species-specific molecular mechanisms enabling environmental adaptations. Recent studies, described in this review, have revealed the emergent complexity of the intricate cross-regulation between c-di-GMP signalling and flagellar motility, highlighting context-specific deviations from simple antagonism and underscoring the importance of studying transient dynamics of c-di-GMP, gene expression, and motility changes during the lifestyle transitions.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"87 ","pages":"Article 102632"},"PeriodicalIF":5.9,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Compartmentalization during bacterial spore formation 细菌孢子形成过程中的区隔化

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-07-08 DOI: 10.1016/j.mib.2025.102633

Olga Iwańska, Przemysław Latoch, Agata L. Starosta

{"title":"Compartmentalization during bacterial spore formation","authors":"Olga Iwańska, Przemysław Latoch, Agata L. Starosta","doi":"10.1016/j.mib.2025.102633","DOIUrl":"10.1016/j.mib.2025.102633","url":null,"abstract":"<div><div>Here, we explore the recent advancements in understanding cellular compartmentalization during bacterial spore formation, primarily focusing on the model organism <em>Bacillus subtilis</em>. The hallmark of sporulation, asymmetric septation, physically separates the mother cell and forespore, enabling distinct developmental fates. We highlight the role of the asymmetric septum as an organizational hub coordinating diverse compartmentalized functions — from gene regulation to metabolism and protein synthesis machinery localization.</div><div>Asymmetric septation involves precise positioning of the division machinery, chromosome segregation, and septal pore formation. Recent studies have revealed detailed structure of the asymmetric septum and its role in maintaining compartment integrity, especially through interactions involving SpoIIE, SpoIIIE, peptidoglycan remodeling enzymes (like the SpoIIDMP complex), and the SpoIIIA-SpoIIQ channel. The asymmetric septum also plays a role in the spatiotemporal localization of ribosomes, with their entry into the forespore being coupled to septal peptidoglycan remodeling. This observation not only demonstrates translational compartmentalization during sporulation but also reveals the uncoupling of transcription and translation processes in <em>B. subtilis</em>. Moreover, the mother cell and forespore establish distinct metabolic roles, as the mother cell supplies essential metabolites to the forespore through the SpoIIIA-SpoIIQ feeding tube channel, supporting the synthesis of the spore structural components necessary for spore maturation.</div><div>Advanced imaging techniques and multi-omics approaches have significantly enhanced our understanding of compartmentalization during sporulation. We conclude by discussing future research directions, including the application of machine learning approaches, expansion of research to nonmodel bacterial species, and exploration of evolutionary aspects of compartmentalization, which may reveal universal mechanisms of microbial organization.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"87 ","pages":"Article 102633"},"PeriodicalIF":5.9,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Encapsulins: catalysis inside a shell 胶囊：壳内的催化作用

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-07-05 DOI: 10.1016/j.mib.2025.102629

Asif Fazal, Tobias W Giessen

引用次数: 0

A tale of two nitrous oxide reductases: a cautionary perspective 两个一氧化二氮还原酶的故事：一个警世的观点

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-07-05 DOI: 10.1016/j.mib.2025.102631

Sukhwan Yoon , Min Joon Song , Michele Laureni

{"title":"A tale of two nitrous oxide reductases: a cautionary perspective","authors":"Sukhwan Yoon , Min Joon Song , Michele Laureni","doi":"10.1016/j.mib.2025.102631","DOIUrl":"10.1016/j.mib.2025.102631","url":null,"abstract":"<div><div>Nitrous oxide reductases (N2OR) are the sole sink of the potent greenhouse gas nitrous oxide (N<sub>2</sub>O) in the environment. Having been studied for decades, N2OR have attracted renewed attention following the discovery of a previously unrecognized clade, now termed clade II. This clade exhibits unexpectedly widespread taxonomic distribution and prevalence across diverse environments, prompting research efforts to define and assign distinct clade-specific traits. In this perspective, we aim to critically review and evaluate dichotomous clade-based classifications, addressing oversimplifications and unresolved ambiguities in linking clade identity to physiological traits like substrate affinity, acid tolerance, and aerotolerance. Growing experimental evidence from N<sub>2</sub>O-reducing isolates and enrichments suggests a general difference in substrate affinity between the clades. Recent discoveries of N<sub>2</sub>O reduction at pH < 5.0 attribute the long-sought acidophilic N<sub>2</sub>O reduction exclusively to organisms possessing clade II <em>nosZ</em>, and attempts have also been made to relate clade separation to aerotolerant N<sub>2</sub>O reduction. However, it is important to note that such binary characterizations are based on limited observations and lack a solid understanding of the underlying mechanisms, exposing them to bias and oversimplification risks. We emphasize the need for a balanced research effort to establish a robust link between ecophysiology and biochemistry, enabling a more accurate evaluation of clade-based characterizations and, ultimately, a deeper understanding and effective harnessing of N<sub>2</sub>O-reducing organisms.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"86 ","pages":"Article 102631"},"PeriodicalIF":5.9,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0