Current opinion in microbiology最新文献_第3页

Induced pluripotent stem cell–based tissue models to study malaria: a new player in the research game 基于诱导多能干细胞的组织模型研究疟疾：研究游戏中的新玩家

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-02-25 DOI: 10.1016/j.mib.2025.102585

François Korbmacher , Maria Bernabeu

{"title":"Induced pluripotent stem cell–based tissue models to study malaria: a new player in the research game","authors":"François Korbmacher , Maria Bernabeu","doi":"10.1016/j.mib.2025.102585","DOIUrl":"10.1016/j.mib.2025.102585","url":null,"abstract":"<div><div>Most <em>in vitro</em> studies on parasite development and pathogenesis in the human host have been conducted using traditional primary or immortalized cells, despite their inherent limitations. Breakthroughs in the field of induced pluripotent stem cells (iPSCs) are revolutionizing disease modeling, offering alternatives to traditional <em>in vivo</em> and <em>in vitro</em> infection models. Human iPSCs differentiate into all cell types, proliferate indefinitely, and offer experimental advantages, like genome editing and donor control. iPSCs can be engineered into complex 3D tissue models that closely mimic morphology and function of their <em>in vivo</em> counterparts and allow for precise experimental manipulation.</div><div>The physiological complexity of iPSC-based tissue models has improved rapidly. Given <em>Plasmodium</em>'s systemic impact across multiple organs, these models provide an invaluable resource for studying parasite–tissue interactions. This opinion article focuses on recent developments of iPSC-based models for <em>Plasmodium</em> research. We describe the main highlights and potential use of these systems while acknowledging current limitations.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"84 ","pages":"Article 102585"},"PeriodicalIF":5.9,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Can a microbial community become an evolutionary individual? 微生物群落能成为一个进化的个体吗？

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-02-20 DOI: 10.1016/j.mib.2025.102596

Afra Salazar , Sara Mitri

引用次数: 0

Targeted isolation of piperazate-containing molecules: bioinformatics and spectroscopy 含哌酸酯分子的靶向分离：生物信息学和光谱学

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-02-16 DOI: 10.1016/j.mib.2025.102584

Mostafa Hagar , Sangwook Kang , Raymond J Andersen , Dong-Chan Oh , Katherine S Ryan

引用次数: 0

Protein families secreted by nematodes to modulate host immunity 线虫分泌调节宿主免疫的蛋白家族

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-02-15 DOI: 10.1016/j.mib.2025.102582

Florent Colomb, Henry J McSorley

引用次数: 0

A CRISPR view on genetic screens in Toxoplasma gondii 刚地弓形虫基因筛选的CRISPR观点。

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-02-01 DOI: 10.1016/j.mib.2024.102577

Franziska Hildebrandt , Ana N. Matias , Moritz Treeck

{"title":"A CRISPR view on genetic screens in Toxoplasma gondii","authors":"Franziska Hildebrandt , Ana N. Matias , Moritz Treeck","doi":"10.1016/j.mib.2024.102577","DOIUrl":"10.1016/j.mib.2024.102577","url":null,"abstract":"<div><div>Genome editing technologies, such as CRISPR-Cas9, have revolutionised the study of genes in a variety of organisms, including unicellular parasites. Today, the CRISPR-Cas9 technology is vastly applied in high-throughput screens to investigate interactions between the Apicomplexan parasite <em>Toxoplasma gondii</em> and its hosts. <em>In vitro</em> and <em>in vivo T. gondii</em> screens performed in naive and restrictive conditions have led to the discovery of essential and fitness-conferring <em>T. gondii</em> genes, as well as factors important for virulence and dissemination. Recent studies have adapted the CRISPR-Cas9 screening technology to study <em>T. gondii</em> genes based on phenotypes unrelated to parasite survival. These advances were achieved by using conditional systems coupled with imaging, as well as single-cell RNA sequencing and phenotypic selection. Here, we review the state-of-the-art of CRISPR-Cas9 screening technologies with a focus on <em>T. gondii</em>, highlighting strengths, current limitations and future avenues for its development, including its application to other Apicomplexan species.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"83 ","pages":"Article 102577"},"PeriodicalIF":5.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gram-positive pathogens, inflammation, and the host lipid environment 革兰氏阳性病原体，炎症和宿主脂质环境

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-02-01 DOI: 10.1016/j.mib.2025.102581

Reginald A Woods , Sarai Guzman Vela , Francis Alonzo III

引用次数: 0

Harnessing gut microbial communities to unravel microbiome functions 利用肠道微生物群落来揭示微生物群的功能。

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-02-01 DOI: 10.1016/j.mib.2024.102578

Samir Giri , Handuo Shi , Athanasios Typas , Kerwyn Casey Huang

{"title":"Harnessing gut microbial communities to unravel microbiome functions","authors":"Samir Giri , Handuo Shi , Athanasios Typas , Kerwyn Casey Huang","doi":"10.1016/j.mib.2024.102578","DOIUrl":"10.1016/j.mib.2024.102578","url":null,"abstract":"<div><div>The gut microbiome impacts human health in direct and indirect ways. While many associations have been discovered between specific microbiome compositions and diseases, establishing causality, understanding the underlying mechanisms, and developing successful microbiome-based therapies require novel experimental approaches. In this opinion, we discuss how <em>in vitro</em> cultivation of diverse communities enables systematic investigation of the individual and collective functions of gut microbes. Up to now, the field has relied mostly on simple, bottom-up assembled synthetic communities or more complex, undefined stool-derived communities. Although powerful for dissecting interactions and mapping causal effects, these communities suffer either from ignoring the complexity, diversity, coevolution, and dynamics of natural communities or from lack of control of community composition. These limitations can be overcome in the future by establishing personalized culture collections from stool samples of different donors and assembling personalized communities to investigate native interactions and ecological relationships in a controlled manner.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"83 ","pages":"Article 102578"},"PeriodicalIF":5.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The architecture of theory and data in microbiome design: towards an S-matrix for microbiomes 微生物组设计的理论与数据架构：迈向微生物组的s矩阵。

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-02-01 DOI: 10.1016/j.mib.2025.102580

Shreya Arya , Ashish B George , James O'Dwyer

{"title":"The architecture of theory and data in microbiome design: towards an S-matrix for microbiomes","authors":"Shreya Arya , Ashish B George , James O'Dwyer","doi":"10.1016/j.mib.2025.102580","DOIUrl":"10.1016/j.mib.2025.102580","url":null,"abstract":"<div><div>Designing microbiomes for applications in health, bioengineering, and sustainability is intrinsically linked to a fundamental theoretical understanding of the rules governing microbial community assembly. Microbial ecologists have used a range of mathematical models to understand, predict, and control microbiomes, ranging from mechanistic models, putting microbial populations and their interactions as the focus, to purely statistical approaches, searching for patterns in empirical and experimental data. We review the success and limitations of these modeling approaches when designing novel microbiomes, especially when guided by (inevitably) incomplete experimental data. Although successful at predicting generic patterns of community assembly, mechanistic and phenomenological models tend to fall short of the precision needed to design and implement specific functionality in a microbiome. We argue that to effectively design microbiomes with optimal functions in diverse environments, ecologists should combine data-driven techniques with mechanistic models — a middle, third way for using theory to inform design.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"83 ","pages":"Article 102580"},"PeriodicalIF":5.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alginate catabolic systems in marine bacteria 海藻酸盐在海洋细菌中的分解代谢系统。

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-02-01 DOI: 10.1016/j.mib.2024.102564

Fei Xu , Xiu-Lan Chen , Yu-Zhong Zhang

引用次数: 0

Revisiting the potential of natural products in antimycobacterial therapy: advances in drug discovery and semisynthetic solutions 重新审视天然产物在抗细菌治疗中的潜力：药物发现和半合成解决方案的进展。

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-02-01 DOI: 10.1016/j.mib.2024.102576

Maya George, Gerard D Wright

{"title":"Revisiting the potential of natural products in antimycobacterial therapy: advances in drug discovery and semisynthetic solutions","authors":"Maya George, Gerard D Wright","doi":"10.1016/j.mib.2024.102576","DOIUrl":"10.1016/j.mib.2024.102576","url":null,"abstract":"<div><div>Natural products have been pivotal in treating mycobacterial infections with early antibiotics such as streptomycin, forming the foundation of tuberculosis therapy. However, the emergence of multidrug-resistant and extensively drug-resistant <em>Mycobacterium</em> species has intensified the need for novel antimycobacterial agents. In this review, we revisit the historical contributions of natural products to antimycobacterial drug discovery and highlight recent advances in the field. We assess the application of molecular networking and the exploration of unculturable bacteria in identifying new antimycobacterial compounds such as amycobactin and levesquamides. We also highlight the role of semisynthesis in optimizing natural products, exemplified by sequanamycins and spectinomycin analogs that evade <em>M. tuberculosis’</em> intrinsic resistance. Finally, we discuss emerging technologies that are promising to accelerate the discovery and development of next-generation antimycobacterial therapies. Despite ongoing challenges, these innovative approaches offer renewed hope in addressing the growing crisis of drug-resistant mycobacterial infections.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"83 ","pages":"Article 102576"},"PeriodicalIF":5.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0