Current opinion in microbiology最新文献

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-04-02 DOI: 10.1016/j.mib.2025.102608

Gaurav Kumar Lohia, Sebastián A Riquelme

{"title":"Pathogen adaptation to lung metabolites","authors":"Gaurav Kumar Lohia, Sebastián A Riquelme","doi":"10.1016/j.mib.2025.102608","DOIUrl":"10.1016/j.mib.2025.102608","url":null,"abstract":"<div><div>Opportunistic pathogens like <em>Pseudomonas aeruginosa</em> and <em>Staphylococcus aureus</em> rapidly adapt to the dynamic metabolic landscape of the respiratory mucosa during infection. Host phagocytes recognize these pathogens and trigger metabolic reprogramming, releasing immunometabolites such as succinate and itaconate. <em>P. aeruginosa</em> preferentially consumes succinate as a carbon source to enhance planktonic growth. In response to itaconate-induced membrane stress, it forms protective biofilms, allowing bacterial survival despite host defenses. Additionally, host ketone bodies support microbial communities that are less immunostimulatory and better tolerated by the lung. Similarly, <em>S. aureus</em> responds to itaconate by forming biofilms, aiding colonization in glucose-limited airways. In this milieu, <em>S. aureus</em> consumes proline, linking its survival with the metabolic activity of proline-producing fibroblasts. Here, we will review the competence of both <em>P. aeruginosa</em> and <em>S. aureus</em> to hijack host metabolic pathways, underscoring pathogen metabolic plasticity as an essential strategy to thrive in the human lung.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"85 ","pages":"Article 102608"},"PeriodicalIF":5.9,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of microbial succinate in the pathophysiology of inflammatory bowel disease: mechanisms and therapeutic potential

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-03-24 DOI: 10.1016/j.mib.2025.102599

Sonia Fernández-Veledo , Carme Grau-Bové , Sara Notararigo , Isabel Huber-Ruano

{"title":"The role of microbial succinate in the pathophysiology of inflammatory bowel disease: mechanisms and therapeutic potential","authors":"Sonia Fernández-Veledo , Carme Grau-Bové , Sara Notararigo , Isabel Huber-Ruano","doi":"10.1016/j.mib.2025.102599","DOIUrl":"10.1016/j.mib.2025.102599","url":null,"abstract":"<div><div>Inflammatory bowel disease (IBD) is a chronic immune-mediated condition linked to gut microbiota dysbiosis and altered production of bacterial metabolites, including succinate, which is also a key intermediate in human mitochondrial energy metabolism in human cells. Succinate levels in the gut are influenced by microbial community dynamics and cross-feeding interactions, highlighting its dual metabolic and ecological importance. Extracellular succinate acts as a key signaling metabolite linking microbial metabolism to host physiology, with transient rises supporting metabolic regulation but chronic elevations contributing to metabolic disorders and disease progression. Succinate signals through its cognate receptor SUCNR1, which mediates adaptive metabolic responses under normal conditions but drives inflammation and fibrosis when dysregulated.</div><div>IBD patients display a dysbiotic gut microbiota characterized by an increased prevalence of succinate-producing bacteria, contributing to elevated succinate levels in the gut and circulation. This imbalance drives inflammation, worsens IBD severity, and contributes to complications like <em>Clostridioides difficile</em> infection and fibrosis. Emerging evidence highlights the potential of intestinal and systemic succinate levels as indicators of microbial dysbiosis, with a bidirectional relationship between microbial composition and succinate metabolism. Understanding the factors influencing succinate levels and their interaction with dysbiosis shows promise in the development of therapeutic strategies to restore microbial balance. Approaches such as dietary fiber enrichment, prebiotics, and probiotics to enhance succinate-consuming bacteria, combined with targeted modulation of succinate pathways (e.g. SDH inhibitors, SUCNR1 antagonists), hold promise for mitigating inflammation and improving gut health in IBD.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"85 ","pages":"Article 102599"},"PeriodicalIF":5.9,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143685137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immune evasion runs in the family: two surface protein families of Plasmodium falciparum–infected erythrocytes

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-03-19 DOI: 10.1016/j.mib.2025.102598

Samuel G Chamberlain , Shiroh Iwanaga , Matthew K Higgins

{"title":"Immune evasion runs in the family: two surface protein families of Plasmodium falciparum–infected erythrocytes","authors":"Samuel G Chamberlain , Shiroh Iwanaga , Matthew K Higgins","doi":"10.1016/j.mib.2025.102598","DOIUrl":"10.1016/j.mib.2025.102598","url":null,"abstract":"<div><div>Two protein families are found on the surfaces of erythrocytes infected with <em>Plasmodium falciparum</em>, a causative agent of deadly malaria. PfEMP1 are tethers binding endothelial receptors and holding infected erythrocytes to tissue and blood vessel surfaces, away from splenic clearance. RIFINs interact with immune receptors on natural killer cells, suppressing infected erythrocyte destruction. Both have expanded into families of diverse members to allow antigenic variation but retain surfaces of conserved chemistry and shape to bind human receptors. Recently discovered broadly inhibitory antibodies target one such surface on many EPCR-binding PfEMP1. Remarkable antibodies take this one step further, directly incorporating ectodomains of immune receptors into their loops, allowing RIFIN recognition. Finally, some RIFINs are targets of activating killer immune receptors, helping natural killer cells destroy infected erythrocytes. Studies of these two families therefore reveal a snapshot of the battle between this ancient parasite and the immune system of its human host.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"85 ","pages":"Article 102598"},"PeriodicalIF":5.9,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How pathogens drive adipose tissue loss in the host

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-03-12 DOI: 10.1016/j.mib.2025.102597

Abdulbasit Amin , David V Ferreira , Luisa M Figueiredo

引用次数: 0

Induced pluripotent stem cell–based tissue models to study malaria: a new player in the research game

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-02-25 DOI: 10.1016/j.mib.2025.102585

François Korbmacher , Maria Bernabeu

{"title":"Induced pluripotent stem cell–based tissue models to study malaria: a new player in the research game","authors":"François Korbmacher , Maria Bernabeu","doi":"10.1016/j.mib.2025.102585","DOIUrl":"10.1016/j.mib.2025.102585","url":null,"abstract":"<div><div>Most <em>in vitro</em> studies on parasite development and pathogenesis in the human host have been conducted using traditional primary or immortalized cells, despite their inherent limitations. Breakthroughs in the field of induced pluripotent stem cells (iPSCs) are revolutionizing disease modeling, offering alternatives to traditional <em>in vivo</em> and <em>in vitro</em> infection models. Human iPSCs differentiate into all cell types, proliferate indefinitely, and offer experimental advantages, like genome editing and donor control. iPSCs can be engineered into complex 3D tissue models that closely mimic morphology and function of their <em>in vivo</em> counterparts and allow for precise experimental manipulation.</div><div>The physiological complexity of iPSC-based tissue models has improved rapidly. Given <em>Plasmodium</em>'s systemic impact across multiple organs, these models provide an invaluable resource for studying parasite–tissue interactions. This opinion article focuses on recent developments of iPSC-based models for <em>Plasmodium</em> research. We describe the main highlights and potential use of these systems while acknowledging current limitations.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"84 ","pages":"Article 102585"},"PeriodicalIF":5.9,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Can a microbial community become an evolutionary individual?

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-02-20 DOI: 10.1016/j.mib.2025.102596

Afra Salazar , Sara Mitri

引用次数: 0

Targeted isolation of piperazate-containing molecules: bioinformatics and spectroscopy

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-02-16 DOI: 10.1016/j.mib.2025.102584

Mostafa Hagar , Sangwook Kang , Raymond J Andersen , Dong-Chan Oh , Katherine S Ryan

引用次数: 0

Protein families secreted by nematodes to modulate host immunity

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-02-15 DOI: 10.1016/j.mib.2025.102582

Florent Colomb, Henry J McSorley

引用次数: 0

A CRISPR view on genetic screens in Toxoplasma gondii 刚地弓形虫基因筛选的CRISPR观点。

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-02-01 DOI: 10.1016/j.mib.2024.102577

Franziska Hildebrandt , Ana N. Matias , Moritz Treeck

{"title":"A CRISPR view on genetic screens in Toxoplasma gondii","authors":"Franziska Hildebrandt , Ana N. Matias , Moritz Treeck","doi":"10.1016/j.mib.2024.102577","DOIUrl":"10.1016/j.mib.2024.102577","url":null,"abstract":"<div><div>Genome editing technologies, such as CRISPR-Cas9, have revolutionised the study of genes in a variety of organisms, including unicellular parasites. Today, the CRISPR-Cas9 technology is vastly applied in high-throughput screens to investigate interactions between the Apicomplexan parasite <em>Toxoplasma gondii</em> and its hosts. <em>In vitro</em> and <em>in vivo T. gondii</em> screens performed in naive and restrictive conditions have led to the discovery of essential and fitness-conferring <em>T. gondii</em> genes, as well as factors important for virulence and dissemination. Recent studies have adapted the CRISPR-Cas9 screening technology to study <em>T. gondii</em> genes based on phenotypes unrelated to parasite survival. These advances were achieved by using conditional systems coupled with imaging, as well as single-cell RNA sequencing and phenotypic selection. Here, we review the state-of-the-art of CRISPR-Cas9 screening technologies with a focus on <em>T. gondii</em>, highlighting strengths, current limitations and future avenues for its development, including its application to other Apicomplexan species.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"83 ","pages":"Article 102577"},"PeriodicalIF":5.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gram-positive pathogens, inflammation, and the host lipid environment

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-02-01 DOI: 10.1016/j.mib.2025.102581

Reginald A Woods , Sarai Guzman Vela , Francis Alonzo III

引用次数: 0