Current opinion in microbiology最新文献

Recent advances in natural and synthetic phosphonate therapeutics 天然和合成膦酸盐疗法的最新进展

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-07-12 DOI: 10.1016/j.mib.2025.102630

Jerry Cui , Kou-San Ju

引用次数: 0

Seeing between the lines: matrix-assisted laser desorption/ionization mass spectrometry imaging advances for microbial interactions 看字里行间：基质辅助激光解吸/电离质谱成像微生物相互作用的进展

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-07-12 DOI: 10.1016/j.mib.2025.102634

Laura Rodriguez-Velandia , Ethan A Older , Laura M Sanchez

{"title":"Seeing between the lines: matrix-assisted laser desorption/ionization mass spectrometry imaging advances for microbial interactions","authors":"Laura Rodriguez-Velandia , Ethan A Older , Laura M Sanchez","doi":"10.1016/j.mib.2025.102634","DOIUrl":"10.1016/j.mib.2025.102634","url":null,"abstract":"<div><div>This review delves into the technical advancements of matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) applications from the last 15 years, highlighting its importance as an analytical tool for exploring the complex interactions within microbial communities. Recognizing the limitations of standard laboratory cultivation practices, we examine how recent research has worked toward bridging the gap between controlled laboratory experiments and <em>in situ</em> measurements. We discuss the shift from traditional agar-based co-culture to newer methods, such as 3D MALDI-MSI and artificial environments, ultimately progressing toward <em>in situ</em> analysis. We highlight the innovative aspects of these methodologies, emphasizing the technical challenges of sample preparation and subsequent metabolite identification. While MALDI-MSI has proven to be an invaluable tool for mapping chemical communication within complex microbial communities, we acknowledge the significant bottlenecks that remain. We call on the researchers to use establish reporting standards and support the expansion of metabolomic databases for future discoveries.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"87 ","pages":"Article 102634"},"PeriodicalIF":5.9,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of nucleoid-associated proteins in mediating responses to environmental changes 核相关蛋白在介导环境变化反应中的作用

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-07-09 DOI: 10.1016/j.mib.2025.102628

Pingzhuang Ge , Fatema-Zahra M Rashid , Remus T Dame

引用次数: 0

Swimming or sessile: the interplay between c-di-GMP signalling and flagellar motility 游动或无根：c-二- gmp信号与鞭毛运动的相互作用

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-07-08 DOI: 10.1016/j.mib.2025.102632

Xuanlin Chen, Liyun Wang, Victor Sourjik

{"title":"Swimming or sessile: the interplay between c-di-GMP signalling and flagellar motility","authors":"Xuanlin Chen, Liyun Wang, Victor Sourjik","doi":"10.1016/j.mib.2025.102632","DOIUrl":"10.1016/j.mib.2025.102632","url":null,"abstract":"<div><div>Bacteria have evolved multiple strategies to thrive in diverse environments. These include the ability to make rapid transitions between motile and sessile lifestyles, either of which might be favoured dependent of the environmental conditions. The central regulator for these lifestyle transitions is the second messenger cyclic dimeric guanosine monophosphate (c-di-GMP), which in general, inhibits flagellar motility and promotes the formation of sessile biofilm communities. Reciprocally, flagellated cells suppress c-di-GMP synthesis or activate its degradation to preserve motility. The interplay between c-di-GMP signalling and motility occurs at multiple levels of regulation, with evolutionarily conserved general principles but species-specific molecular mechanisms enabling environmental adaptations. Recent studies, described in this review, have revealed the emergent complexity of the intricate cross-regulation between c-di-GMP signalling and flagellar motility, highlighting context-specific deviations from simple antagonism and underscoring the importance of studying transient dynamics of c-di-GMP, gene expression, and motility changes during the lifestyle transitions.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"87 ","pages":"Article 102632"},"PeriodicalIF":5.9,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Compartmentalization during bacterial spore formation 细菌孢子形成过程中的区隔化

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-07-08 DOI: 10.1016/j.mib.2025.102633

Olga Iwańska, Przemysław Latoch, Agata L. Starosta

{"title":"Compartmentalization during bacterial spore formation","authors":"Olga Iwańska, Przemysław Latoch, Agata L. Starosta","doi":"10.1016/j.mib.2025.102633","DOIUrl":"10.1016/j.mib.2025.102633","url":null,"abstract":"<div><div>Here, we explore the recent advancements in understanding cellular compartmentalization during bacterial spore formation, primarily focusing on the model organism <em>Bacillus subtilis</em>. The hallmark of sporulation, asymmetric septation, physically separates the mother cell and forespore, enabling distinct developmental fates. We highlight the role of the asymmetric septum as an organizational hub coordinating diverse compartmentalized functions — from gene regulation to metabolism and protein synthesis machinery localization.</div><div>Asymmetric septation involves precise positioning of the division machinery, chromosome segregation, and septal pore formation. Recent studies have revealed detailed structure of the asymmetric septum and its role in maintaining compartment integrity, especially through interactions involving SpoIIE, SpoIIIE, peptidoglycan remodeling enzymes (like the SpoIIDMP complex), and the SpoIIIA-SpoIIQ channel. The asymmetric septum also plays a role in the spatiotemporal localization of ribosomes, with their entry into the forespore being coupled to septal peptidoglycan remodeling. This observation not only demonstrates translational compartmentalization during sporulation but also reveals the uncoupling of transcription and translation processes in <em>B. subtilis</em>. Moreover, the mother cell and forespore establish distinct metabolic roles, as the mother cell supplies essential metabolites to the forespore through the SpoIIIA-SpoIIQ feeding tube channel, supporting the synthesis of the spore structural components necessary for spore maturation.</div><div>Advanced imaging techniques and multi-omics approaches have significantly enhanced our understanding of compartmentalization during sporulation. We conclude by discussing future research directions, including the application of machine learning approaches, expansion of research to nonmodel bacterial species, and exploration of evolutionary aspects of compartmentalization, which may reveal universal mechanisms of microbial organization.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"87 ","pages":"Article 102633"},"PeriodicalIF":5.9,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Encapsulins: catalysis inside a shell 胶囊：壳内的催化作用

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-07-05 DOI: 10.1016/j.mib.2025.102629

Asif Fazal, Tobias W Giessen

引用次数: 0

A tale of two nitrous oxide reductases: a cautionary perspective 两个一氧化二氮还原酶的故事：一个警世的观点

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-07-05 DOI: 10.1016/j.mib.2025.102631

Sukhwan Yoon , Min Joon Song , Michele Laureni

{"title":"A tale of two nitrous oxide reductases: a cautionary perspective","authors":"Sukhwan Yoon , Min Joon Song , Michele Laureni","doi":"10.1016/j.mib.2025.102631","DOIUrl":"10.1016/j.mib.2025.102631","url":null,"abstract":"<div><div>Nitrous oxide reductases (N2OR) are the sole sink of the potent greenhouse gas nitrous oxide (N<sub>2</sub>O) in the environment. Having been studied for decades, N2OR have attracted renewed attention following the discovery of a previously unrecognized clade, now termed clade II. This clade exhibits unexpectedly widespread taxonomic distribution and prevalence across diverse environments, prompting research efforts to define and assign distinct clade-specific traits. In this perspective, we aim to critically review and evaluate dichotomous clade-based classifications, addressing oversimplifications and unresolved ambiguities in linking clade identity to physiological traits like substrate affinity, acid tolerance, and aerotolerance. Growing experimental evidence from N<sub>2</sub>O-reducing isolates and enrichments suggests a general difference in substrate affinity between the clades. Recent discoveries of N<sub>2</sub>O reduction at pH < 5.0 attribute the long-sought acidophilic N<sub>2</sub>O reduction exclusively to organisms possessing clade II <em>nosZ</em>, and attempts have also been made to relate clade separation to aerotolerant N<sub>2</sub>O reduction. However, it is important to note that such binary characterizations are based on limited observations and lack a solid understanding of the underlying mechanisms, exposing them to bias and oversimplification risks. We emphasize the need for a balanced research effort to establish a robust link between ecophysiology and biochemistry, enabling a more accurate evaluation of clade-based characterizations and, ultimately, a deeper understanding and effective harnessing of N<sub>2</sub>O-reducing organisms.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"86 ","pages":"Article 102631"},"PeriodicalIF":5.9,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skin models for studying vector-borne kinetoplastid infections 研究媒介传播的着丝体感染的皮肤模型

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-06-13 DOI: 10.1016/j.mib.2025.102617

Laura Hauf , Markus Engstler

{"title":"Skin models for studying vector-borne kinetoplastid infections","authors":"Laura Hauf , Markus Engstler","doi":"10.1016/j.mib.2025.102617","DOIUrl":"10.1016/j.mib.2025.102617","url":null,"abstract":"<div><div>Kinetoplastid infections, caused by <em>Leishmania</em> and <em>Trypanosoma</em> species, pose significant global health challenges, disproportionally affecting vulnerable populations in tropical regions. Despite the skin’s pivotal role as both an entry point and a reservoir for these parasites, the mechanistic understanding of host–parasite interactions at this interface remains limited.</div><div>Recent advancements in bioengineered skin models, such as full thickness skin equivalents and skin organoids, provide a promising complement to <em>in vivo</em> and <em>ex vivo</em> models. These <em>in vitro</em> systems address key challenges related to accessibility, reproducibility, and anatomical relevance, while potentially incorporating key tissue components, including immune cells and vascular structures. By replicating the complex structure of human skin at customizable levels of complexity, they offer powerful platforms for high-resolution studies of parasite–host interactions. Furthermore, by supporting natural vector transmission and enabling the simulation of diverse biological conditions, these systems open new avenues for investigating parasite development, tissue invasion, dissemination and immune dynamics.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"86 ","pages":"Article 102617"},"PeriodicalIF":5.9,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell cycle regulation in Escherichia coli: from governing principles, checkpoints, and control variables to molecular mechanisms 大肠杆菌的细胞周期调控：从控制原则、检查点和控制变量到分子机制

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-06-07 DOI: 10.1016/j.mib.2025.102616

Alix Meunier, Sander K Govers

引用次数: 0

The importance of persistence and dormancy in Trypanosoma cruzi infection and Chagas disease 克氏锥虫感染和恰加斯病持续和休眠的重要性

IF 5.9 2区生物学

Current opinion in microbiology Pub Date : 2025-06-06 DOI: 10.1016/j.mib.2025.102615

Molly E Bunkofske , Fernando J Sanchez-Valdez , Rick L Tarleton

引用次数: 0