看字里行间:基质辅助激光解吸/电离质谱成像微生物相互作用的进展

IF 5.9 2区 生物学 Q1 MICROBIOLOGY
Laura Rodriguez-Velandia , Ethan A Older , Laura M Sanchez
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引用次数: 0

摘要

本文综述了过去15年来基质辅助激光解吸/电离质谱成像(MALDI-MSI)应用的技术进展,强调了其作为探索微生物群落内复杂相互作用的分析工具的重要性。认识到标准实验室培养实践的局限性,我们研究了最近的研究如何在实验室控制实验和原位测量之间架起桥梁。我们讨论了从传统的基于琼脂的共培养到新方法的转变,如3D MALDI-MSI和人工环境,最终向原位分析发展。我们强调这些方法的创新方面,强调样品制备和随后的代谢物鉴定的技术挑战。虽然MALDI-MSI已被证明是绘制复杂微生物群落内化学通讯的宝贵工具,但我们承认仍然存在重大瓶颈。我们呼吁研究人员使用建立的报告标准,并支持代谢组学数据库的扩展,以用于未来的发现。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Seeing between the lines: matrix-assisted laser desorption/ionization mass spectrometry imaging advances for microbial interactions
This review delves into the technical advancements of matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) applications from the last 15 years, highlighting its importance as an analytical tool for exploring the complex interactions within microbial communities. Recognizing the limitations of standard laboratory cultivation practices, we examine how recent research has worked toward bridging the gap between controlled laboratory experiments and in situ measurements. We discuss the shift from traditional agar-based co-culture to newer methods, such as 3D MALDI-MSI and artificial environments, ultimately progressing toward in situ analysis. We highlight the innovative aspects of these methodologies, emphasizing the technical challenges of sample preparation and subsequent metabolite identification. While MALDI-MSI has proven to be an invaluable tool for mapping chemical communication within complex microbial communities, we acknowledge the significant bottlenecks that remain. We call on the researchers to use establish reporting standards and support the expansion of metabolomic databases for future discoveries.
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来源期刊
Current opinion in microbiology
Current opinion in microbiology 生物-微生物学
CiteScore
10.00
自引率
0.00%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Current Opinion in Microbiology is a systematic review journal that aims to provide specialists with a unique and educational platform to keep up-to-date with the expanding volume of information published in the field of microbiology. It consists of 6 issues per year covering the following 11 sections, each of which is reviewed once a year: Host-microbe interactions: bacteria Cell regulation Environmental microbiology Host-microbe interactions: fungi/parasites/viruses Antimicrobials Microbial systems biology Growth and development: eukaryotes/prokaryotes
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