Laura Rodriguez-Velandia , Ethan A Older , Laura M Sanchez
{"title":"看字里行间:基质辅助激光解吸/电离质谱成像微生物相互作用的进展","authors":"Laura Rodriguez-Velandia , Ethan A Older , Laura M Sanchez","doi":"10.1016/j.mib.2025.102634","DOIUrl":null,"url":null,"abstract":"<div><div>This review delves into the technical advancements of matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) applications from the last 15 years, highlighting its importance as an analytical tool for exploring the complex interactions within microbial communities. Recognizing the limitations of standard laboratory cultivation practices, we examine how recent research has worked toward bridging the gap between controlled laboratory experiments and <em>in situ</em> measurements. We discuss the shift from traditional agar-based co-culture to newer methods, such as 3D MALDI-MSI and artificial environments, ultimately progressing toward <em>in situ</em> analysis. We highlight the innovative aspects of these methodologies, emphasizing the technical challenges of sample preparation and subsequent metabolite identification. While MALDI-MSI has proven to be an invaluable tool for mapping chemical communication within complex microbial communities, we acknowledge the significant bottlenecks that remain. We call on the researchers to use establish reporting standards and support the expansion of metabolomic databases for future discoveries.</div></div>","PeriodicalId":10921,"journal":{"name":"Current opinion in microbiology","volume":"87 ","pages":"Article 102634"},"PeriodicalIF":5.9000,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Seeing between the lines: matrix-assisted laser desorption/ionization mass spectrometry imaging advances for microbial interactions\",\"authors\":\"Laura Rodriguez-Velandia , Ethan A Older , Laura M Sanchez\",\"doi\":\"10.1016/j.mib.2025.102634\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>This review delves into the technical advancements of matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) applications from the last 15 years, highlighting its importance as an analytical tool for exploring the complex interactions within microbial communities. Recognizing the limitations of standard laboratory cultivation practices, we examine how recent research has worked toward bridging the gap between controlled laboratory experiments and <em>in situ</em> measurements. We discuss the shift from traditional agar-based co-culture to newer methods, such as 3D MALDI-MSI and artificial environments, ultimately progressing toward <em>in situ</em> analysis. We highlight the innovative aspects of these methodologies, emphasizing the technical challenges of sample preparation and subsequent metabolite identification. While MALDI-MSI has proven to be an invaluable tool for mapping chemical communication within complex microbial communities, we acknowledge the significant bottlenecks that remain. We call on the researchers to use establish reporting standards and support the expansion of metabolomic databases for future discoveries.</div></div>\",\"PeriodicalId\":10921,\"journal\":{\"name\":\"Current opinion in microbiology\",\"volume\":\"87 \",\"pages\":\"Article 102634\"},\"PeriodicalIF\":5.9000,\"publicationDate\":\"2025-07-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current opinion in microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1369527425000566\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current opinion in microbiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1369527425000566","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Seeing between the lines: matrix-assisted laser desorption/ionization mass spectrometry imaging advances for microbial interactions
This review delves into the technical advancements of matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) applications from the last 15 years, highlighting its importance as an analytical tool for exploring the complex interactions within microbial communities. Recognizing the limitations of standard laboratory cultivation practices, we examine how recent research has worked toward bridging the gap between controlled laboratory experiments and in situ measurements. We discuss the shift from traditional agar-based co-culture to newer methods, such as 3D MALDI-MSI and artificial environments, ultimately progressing toward in situ analysis. We highlight the innovative aspects of these methodologies, emphasizing the technical challenges of sample preparation and subsequent metabolite identification. While MALDI-MSI has proven to be an invaluable tool for mapping chemical communication within complex microbial communities, we acknowledge the significant bottlenecks that remain. We call on the researchers to use establish reporting standards and support the expansion of metabolomic databases for future discoveries.
期刊介绍:
Current Opinion in Microbiology is a systematic review journal that aims to provide specialists with a unique and educational platform to keep up-to-date with the expanding volume of information published in the field of microbiology. It consists of 6 issues per year covering the following 11 sections, each of which is reviewed once a year:
Host-microbe interactions: bacteria
Cell regulation
Environmental microbiology
Host-microbe interactions: fungi/parasites/viruses
Antimicrobials
Microbial systems biology
Growth and development: eukaryotes/prokaryotes