Current Genetics最新文献_第8页

Application of recyclable CRISPR/Cas9 tools for targeted genome editing in the postharvest pathogenic fungi Penicillium digitatum and Penicillium expansum 可回收CRISPR/Cas9工具在采收后致病真菌指状青霉和扩张青霉靶向基因组编辑中的应用

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-03-17 DOI: 10.1007/s00294-022-01236-0

Sandra Garrigues, P. Manzanares, J. Marcos

引用次数: 6

TAL effectors and the predicted host targets of pomegranate bacterial blight pathogen Xanthomonas citri pv. punicae 石榴白叶枯病病原黄单胞菌的TAL效应物和预测宿主靶标。双关语

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-03-11 DOI: 10.1007/s00294-022-01232-4

Sruthi Sivaraman, Dineshkumar Krishnamoorthy, K. Arvind, T. Grace, J. Sharma, Ginny Antony

引用次数: 4

Increased peroxisome proliferation is associated with early yeast replicative ageing 过氧化物酶体增殖增加与酵母菌早期复制老化有关

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-27 DOI: 10.1007/s00294-022-01233-3

Rachayeeta Deb, Suchetana Ghose, Shirisha Nagotu

引用次数: 5

Deciphering β-tubulin gene of carbendazim resistant Fusarium solani isolate and its comparison with other Fusarium species 耐多菌灵番茄枯萎菌分离株β-微管蛋白基因的解析及其与其它枯萎菌的比较

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-15 DOI: 10.1007/s00294-022-01238-y

Mrinmay Tarafder, Bejoysekhar Datta

引用次数: 0

Correction to: Genetic response to nitrogen starvation in the aggressive Eucalyptus foliar pathogen Teratosphaeria destructans. 更正:桉树叶片致病菌破坏性畸胎菌对氮饥饿的遗传反应。

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-01 DOI: 10.1007/s00294-021-01216-w

Minette Havenga, Brenda D Wingfield, Michael J Wingfield, Léanne L Dreyer, Francois Roets, Janneke Aylward

引用次数: 0

Off-target effects of base editors: what we know and how we can reduce it. 碱基编辑器的脱靶效应:我们知道什么以及如何减少它。

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-01 Epub Date: 2021-09-13 DOI: 10.1007/s00294-021-01211-1

Yana S Slesarenko, Alexander V Lavrov, Svetlana A Smirnikhina

{"title":"Off-target effects of base editors: what we know and how we can reduce it.","authors":"Yana S Slesarenko, Alexander V Lavrov, Svetlana A Smirnikhina","doi":"10.1007/s00294-021-01211-1","DOIUrl":"https://doi.org/10.1007/s00294-021-01211-1","url":null,"abstract":"The recently discovered CRISPR-Cas9 modification, base editors (BEs), is considered as one of the most promising tools for correcting disease-causing mutations in humans, since it allows point substitutions to be edited without generating double-stranded DNA breaks, and, therefore, with a significant decrease in non-specific activity. Until recently, this method was considered the safest, but at the same time, it is quite effective. However, recent studies of non-specific activity of BEs revealed that some of them lead to the formation of a huge number of off-targets in both DNA and RNA, occurring due to the nature of the Cas9-fused proteins used. In this review article, we have considered and combined data from numerous studies about the most commonly used and more described in detail APOBEC-based BEs and Target-AID version of CBE, as well as ABE7 and ABE8 with their basic modifications into TadA to improve BEs' specificity. In our opinion, modern advances in molecular genetics make it possible to dramatically reduce the off-target activity of base editors due to introducing mutations into the domains of deaminases or inhibition of Cas9 by anti-CRISPR proteins, which returns BEs to the leading position in genome editing technologies.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39411276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Maturation and shuttling of the yeast telomerase RNP: assembling something new using recycled parts. 酵母端粒酶RNP的成熟和穿梭:利用回收的部分组装新的东西。

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-01 Epub Date: 2021-09-02 DOI: 10.1007/s00294-021-01210-2

Louise Bartle, Yulia Vasianovich, Raymund J Wellinger

{"title":"Maturation and shuttling of the yeast telomerase RNP: assembling something new using recycled parts.","authors":"Louise Bartle, Yulia Vasianovich, Raymund J Wellinger","doi":"10.1007/s00294-021-01210-2","DOIUrl":"https://doi.org/10.1007/s00294-021-01210-2","url":null,"abstract":"As the limiting component of the budding yeast telomerase, the Tlc1 RNA must undergo multiple consecutive modifications and rigorous quality checks throughout its lifecycle. These steps will ensure that only correctly processed and matured molecules are assembled into telomerase complexes that subsequently act at telomeres. The complex pathway of Tlc1 RNA maturation, involving 5'- and 3'-end processing, stabilisation and assembly with the protein subunits, requires at least one nucleo-cytoplasmic passage. Furthermore, it appears that the pathway is tightly coordinated with the association of various and changing proteins, including the export factor Xpo1, the Mex67/Mtr2 complex, the Kap122 importin, the Sm7 ring and possibly the CBC and TREX-1 complexes. Although many of these maturation processes also affect other RNA species, the Tlc1 RNA exploits them in a new combination and, therefore, ultimately follows its own and unique pathway. In this review, we highlight recent new insights in maturation and subcellular shuttling of the budding yeast telomerase RNA and discuss how these events may be fine-tuned by the biochemical characteristics of the varying processing and transport factors as well as the final telomerase components. Finally, we indicate outstanding questions that we feel are important to be addressed for a complete understanding of the telomerase RNA lifecycle and that could have implications for the human telomerase as well.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39380940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Synchronisation of glycolytic activity in yeast cells. 酵母细胞糖酵解活性的同步性。

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-01 Epub Date: 2021-10-11 DOI: 10.1007/s00294-021-01214-y

Marcus J B Hauser

{"title":"Synchronisation of glycolytic activity in yeast cells.","authors":"Marcus J B Hauser","doi":"10.1007/s00294-021-01214-y","DOIUrl":"https://doi.org/10.1007/s00294-021-01214-y","url":null,"abstract":"Glycolysis is the central metabolic pathway of almost every cell and organism. Under appropriate conditions, glycolytic oscillations may occur in individual cells as well as in entire cell populations or tissues. In many biological systems, glycolytic oscillations drive coherent oscillations of other metabolites, for instance in cardiomyocytes near anorexia, or in pancreas where they lead to a pulsatile release of insulin. Oscillations at the population or tissue level require the cells to synchronize their metabolism. We review the progress achieved in studying a model organism for glycolytic oscillations, namely yeast. Oscillations may occur on the level of individual cells as well as on the level of the cell population. In yeast, the cell-to-cell interaction is realized by diffusion-mediated intercellular communication via a messenger molecule. The present mini-review focuses on the synchronisation of glycolytic oscillations in yeast. Synchronisation is a quorum-sensing phenomenon because the collective oscillatory behaviour of a yeast cell population ceases when the cell density falls below a threshold. We review the question, under which conditions individual cells in a sparse population continue or cease to oscillate. Furthermore, we provide an overview of the pathway leading to the onset of synchronized oscillations. We also address the effects of spatial inhomogeneities (e.g., the formation of spatial clusters) on the collective dynamics, and also review the emergence of travelling waves of glycolytic activity. Finally, we briefly review the approaches used in numerical modelling of synchronized cell populations.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39506498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Transcriptional repressor Gal80 recruits corepressor complex Cyc8-Tup1 to structural genes of the Saccharomyces cerevisiae GAL regulon. 转录抑制因子Gal80将辅抑制因子复合物Cyc8-Tup1引入酿酒酵母GAL调控的结构基因。

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-01 Epub Date: 2021-10-07 DOI: 10.1007/s00294-021-01215-x

Julia Lettow, Rasha Aref, Hans-Joachim Schüller

{"title":"Transcriptional repressor Gal80 recruits corepressor complex Cyc8-Tup1 to structural genes of the Saccharomyces cerevisiae GAL regulon.","authors":"Julia Lettow, Rasha Aref, Hans-Joachim Schüller","doi":"10.1007/s00294-021-01215-x","DOIUrl":"https://doi.org/10.1007/s00294-021-01215-x","url":null,"abstract":"Under non-inducing conditions (absence of galactose), yeast structural genes of the GAL regulon are repressed by Gal80, preventing interaction of Gal4 bound to UASGAL promoter motifs with general factors of the transcriptional machinery. In this work, we show that Gal80 is also able to interact with histone deacetylase-recruiting corepressor proteins Cyc8 and Tup1, indicating an additional mechanism of gene repression. This is supported by our demonstration that a lexA-Gal80 fusion efficiently mediates repression of a reporter gene with an upstream lexA operator sequence. Corepressor interaction and in vivo gene repression could be mapped to a Gal80 minimal domain of 65 amino acids (aa 81-145). Site-directed mutagenesis of selected residues within this domain showed that a cluster of aromatic-hydrophobic amino acids (YLFV, aa 118-121) is important, although not solely responsible, for gene repression. Using chromatin immunoprecipitation, Cyc8 and Tup1 were shown to be present at the GAL1 promoter in a wild-type strain but not in a gal80 mutant strain under non-inducing (derepressing) growth conditions. Expression of a GAL1-lacZ fusion was elevated in a tup1 mutant (but not in a cyc8 mutant) grown in derepressing medium, indicating that Tup1 may be mainly responsible for this second mechanism of Gal80-dependent gene repression.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39498766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

The assembly of a noncanonical LINC complex in Saccharomyces cerevisiae. 酿酒酵母中非典型LINC复合物的组装。

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-01 Epub Date: 2021-11-15 DOI: 10.1007/s00294-021-01220-0

Jinbo Fan, Zhuo Sun, Yang Wang

引用次数: 2