Current Genetics最新文献_第10页

Deciphering β-tubulin gene of carbendazim resistant Fusarium solani isolate and its comparison with other Fusarium species 耐多菌灵番茄枯萎菌分离株β-微管蛋白基因的解析及其与其它枯萎菌的比较

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-15 DOI: 10.1007/s00294-022-01238-y

Mrinmay Tarafder, Bejoysekhar Datta

引用次数: 0

Correction to: Genetic response to nitrogen starvation in the aggressive Eucalyptus foliar pathogen Teratosphaeria destructans. 更正:桉树叶片致病菌破坏性畸胎菌对氮饥饿的遗传反应。

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-01 DOI: 10.1007/s00294-021-01216-w

Minette Havenga, Brenda D Wingfield, Michael J Wingfield, Léanne L Dreyer, Francois Roets, Janneke Aylward

引用次数: 0

Off-target effects of base editors: what we know and how we can reduce it. 碱基编辑器的脱靶效应:我们知道什么以及如何减少它。

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-01 Epub Date: 2021-09-13 DOI: 10.1007/s00294-021-01211-1

Yana S Slesarenko, Alexander V Lavrov, Svetlana A Smirnikhina

{"title":"Off-target effects of base editors: what we know and how we can reduce it.","authors":"Yana S Slesarenko, Alexander V Lavrov, Svetlana A Smirnikhina","doi":"10.1007/s00294-021-01211-1","DOIUrl":"https://doi.org/10.1007/s00294-021-01211-1","url":null,"abstract":"The recently discovered CRISPR-Cas9 modification, base editors (BEs), is considered as one of the most promising tools for correcting disease-causing mutations in humans, since it allows point substitutions to be edited without generating double-stranded DNA breaks, and, therefore, with a significant decrease in non-specific activity. Until recently, this method was considered the safest, but at the same time, it is quite effective. However, recent studies of non-specific activity of BEs revealed that some of them lead to the formation of a huge number of off-targets in both DNA and RNA, occurring due to the nature of the Cas9-fused proteins used. In this review article, we have considered and combined data from numerous studies about the most commonly used and more described in detail APOBEC-based BEs and Target-AID version of CBE, as well as ABE7 and ABE8 with their basic modifications into TadA to improve BEs' specificity. In our opinion, modern advances in molecular genetics make it possible to dramatically reduce the off-target activity of base editors due to introducing mutations into the domains of deaminases or inhibition of Cas9 by anti-CRISPR proteins, which returns BEs to the leading position in genome editing technologies.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":" ","pages":"39-48"},"PeriodicalIF":2.5,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39411276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Maturation and shuttling of the yeast telomerase RNP: assembling something new using recycled parts. 酵母端粒酶RNP的成熟和穿梭:利用回收的部分组装新的东西。

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-01 Epub Date: 2021-09-02 DOI: 10.1007/s00294-021-01210-2

Louise Bartle, Yulia Vasianovich, Raymund J Wellinger

{"title":"Maturation and shuttling of the yeast telomerase RNP: assembling something new using recycled parts.","authors":"Louise Bartle, Yulia Vasianovich, Raymund J Wellinger","doi":"10.1007/s00294-021-01210-2","DOIUrl":"https://doi.org/10.1007/s00294-021-01210-2","url":null,"abstract":"As the limiting component of the budding yeast telomerase, the Tlc1 RNA must undergo multiple consecutive modifications and rigorous quality checks throughout its lifecycle. These steps will ensure that only correctly processed and matured molecules are assembled into telomerase complexes that subsequently act at telomeres. The complex pathway of Tlc1 RNA maturation, involving 5'- and 3'-end processing, stabilisation and assembly with the protein subunits, requires at least one nucleo-cytoplasmic passage. Furthermore, it appears that the pathway is tightly coordinated with the association of various and changing proteins, including the export factor Xpo1, the Mex67/Mtr2 complex, the Kap122 importin, the Sm7 ring and possibly the CBC and TREX-1 complexes. Although many of these maturation processes also affect other RNA species, the Tlc1 RNA exploits them in a new combination and, therefore, ultimately follows its own and unique pathway. In this review, we highlight recent new insights in maturation and subcellular shuttling of the budding yeast telomerase RNA and discuss how these events may be fine-tuned by the biochemical characteristics of the varying processing and transport factors as well as the final telomerase components. Finally, we indicate outstanding questions that we feel are important to be addressed for a complete understanding of the telomerase RNA lifecycle and that could have implications for the human telomerase as well.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":" ","pages":"3-14"},"PeriodicalIF":2.5,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39380940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Synchronisation of glycolytic activity in yeast cells. 酵母细胞糖酵解活性的同步性。

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-01 Epub Date: 2021-10-11 DOI: 10.1007/s00294-021-01214-y

Marcus J B Hauser

{"title":"Synchronisation of glycolytic activity in yeast cells.","authors":"Marcus J B Hauser","doi":"10.1007/s00294-021-01214-y","DOIUrl":"https://doi.org/10.1007/s00294-021-01214-y","url":null,"abstract":"Glycolysis is the central metabolic pathway of almost every cell and organism. Under appropriate conditions, glycolytic oscillations may occur in individual cells as well as in entire cell populations or tissues. In many biological systems, glycolytic oscillations drive coherent oscillations of other metabolites, for instance in cardiomyocytes near anorexia, or in pancreas where they lead to a pulsatile release of insulin. Oscillations at the population or tissue level require the cells to synchronize their metabolism. We review the progress achieved in studying a model organism for glycolytic oscillations, namely yeast. Oscillations may occur on the level of individual cells as well as on the level of the cell population. In yeast, the cell-to-cell interaction is realized by diffusion-mediated intercellular communication via a messenger molecule. The present mini-review focuses on the synchronisation of glycolytic oscillations in yeast. Synchronisation is a quorum-sensing phenomenon because the collective oscillatory behaviour of a yeast cell population ceases when the cell density falls below a threshold. We review the question, under which conditions individual cells in a sparse population continue or cease to oscillate. Furthermore, we provide an overview of the pathway leading to the onset of synchronized oscillations. We also address the effects of spatial inhomogeneities (e.g., the formation of spatial clusters) on the collective dynamics, and also review the emergence of travelling waves of glycolytic activity. Finally, we briefly review the approaches used in numerical modelling of synchronized cell populations.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":" ","pages":"69-81"},"PeriodicalIF":2.5,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39506498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

The assembly of a noncanonical LINC complex in Saccharomyces cerevisiae. 酿酒酵母中非典型LINC复合物的组装。

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-01 Epub Date: 2021-11-15 DOI: 10.1007/s00294-021-01220-0

Jinbo Fan, Zhuo Sun, Yang Wang

引用次数: 2

Transcriptional repressor Gal80 recruits corepressor complex Cyc8-Tup1 to structural genes of the Saccharomyces cerevisiae GAL regulon. 转录抑制因子Gal80将辅抑制因子复合物Cyc8-Tup1引入酿酒酵母GAL调控的结构基因。

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-01 Epub Date: 2021-10-07 DOI: 10.1007/s00294-021-01215-x

Julia Lettow, Rasha Aref, Hans-Joachim Schüller

{"title":"Transcriptional repressor Gal80 recruits corepressor complex Cyc8-Tup1 to structural genes of the Saccharomyces cerevisiae GAL regulon.","authors":"Julia Lettow, Rasha Aref, Hans-Joachim Schüller","doi":"10.1007/s00294-021-01215-x","DOIUrl":"https://doi.org/10.1007/s00294-021-01215-x","url":null,"abstract":"Under non-inducing conditions (absence of galactose), yeast structural genes of the GAL regulon are repressed by Gal80, preventing interaction of Gal4 bound to UASGAL promoter motifs with general factors of the transcriptional machinery. In this work, we show that Gal80 is also able to interact with histone deacetylase-recruiting corepressor proteins Cyc8 and Tup1, indicating an additional mechanism of gene repression. This is supported by our demonstration that a lexA-Gal80 fusion efficiently mediates repression of a reporter gene with an upstream lexA operator sequence. Corepressor interaction and in vivo gene repression could be mapped to a Gal80 minimal domain of 65 amino acids (aa 81-145). Site-directed mutagenesis of selected residues within this domain showed that a cluster of aromatic-hydrophobic amino acids (YLFV, aa 118-121) is important, although not solely responsible, for gene repression. Using chromatin immunoprecipitation, Cyc8 and Tup1 were shown to be present at the GAL1 promoter in a wild-type strain but not in a gal80 mutant strain under non-inducing (derepressing) growth conditions. Expression of a GAL1-lacZ fusion was elevated in a tup1 mutant (but not in a cyc8 mutant) grown in derepressing medium, indicating that Tup1 may be mainly responsible for this second mechanism of Gal80-dependent gene repression.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":" ","pages":"115-124"},"PeriodicalIF":2.5,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39498766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Serine-arginine protein kinase-like protein, SrpkF, stimulates both cellobiose-responsive and D-xylose-responsive signaling pathways in Aspergillus aculeatus. 丝氨酸精氨酸蛋白激酶样蛋白SrpkF刺激针孔曲霉中纤维二糖响应和d -木糖响应的信号通路。

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-01 Epub Date: 2021-08-28 DOI: 10.1007/s00294-021-01207-x

Ryohei Katayama, Natsumi Kobayashi, Takashi Kawaguchi, Shuji Tani

{"title":"Serine-arginine protein kinase-like protein, SrpkF, stimulates both cellobiose-responsive and D-xylose-responsive signaling pathways in Aspergillus aculeatus.","authors":"Ryohei Katayama, Natsumi Kobayashi, Takashi Kawaguchi, Shuji Tani","doi":"10.1007/s00294-021-01207-x","DOIUrl":"https://doi.org/10.1007/s00294-021-01207-x","url":null,"abstract":"Aspergillus aculeatus produces cellulolytic enzymes in the presence of their substrates. We screened a library of 12,000 A. aculeatus T-DNA-inserted mutants to identify a regulatory factor involved in the expression of their enzyme genes in response to inducers. We found one mutant that reduced the expression of FIII-avicelase (chbI) in response to cellulose. T-DNA was inserted into a putative protein kinase gene similar to AN10082 in A. nidulans, serine-arginine protein kinase F, SrpkF. Fold increases in srpkF gene expression in response to various carbon sources were 2.3 (D-xylose), 44 (Avicel®), 59 (Bacto™ Tryptone), and 98 (no carbon) compared with D-glucose. Deletion of srpkF in A. aculeatus resulted in a significant reduction in cellulose-responsive expression of chbI, hydrocellulase (cel7b), and FIb-xylanase (xynIb) genes at an early induction phase. Further, the srpkF-overexpressing strain showed upregulation of the srpkF gene from four- to nine-fold higher than in the control strain. srpkF overexpression upregulated cbhI and cel7b in response to cellobiose and the FI-carboxymethyl cellulase gene (cmc1) and xynIb in response to D-xylose. However, the srpkF deletion did not affect the expression of xynIb in response to D-xylose due to the less expression of srpkF under the D-xylose condition. Our data demonstrate that SrpkF is primarily involved in cellulose-responsive expression, though it has a potential to stimulate gene expression in response to both cellobiose and D-xylose in A. aculeatus.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":" ","pages":"143-152"},"PeriodicalIF":2.5,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00294-021-01207-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39363889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

RNA-cleaving DNAzymes as a diagnostic and therapeutic agent against antimicrobial resistant bacteria. rna切割DNAzymes作为抗微生物耐药菌的诊断和治疗药物。

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-01 Epub Date: 2021-09-09 DOI: 10.1007/s00294-021-01212-0

Bao Chi Wong, Juwaini Abu Bakar, Amreeta Dhanoa, Hock Siew Tan

引用次数: 2

Genetic system underlying responses of Cryptococcus neoformans to cadmium. 新型隐球菌对镉反应的遗传系统。

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-01 Epub Date: 2021-11-10 DOI: 10.1007/s00294-021-01222-y

Akio Toh-E, Misako Ohkusu, Naruhiko Ishiwada, Akira Watanabe, Katsuhiko Kamei

{"title":"Genetic system underlying responses of Cryptococcus neoformans to cadmium.","authors":"Akio Toh-E, Misako Ohkusu, Naruhiko Ishiwada, Akira Watanabe, Katsuhiko Kamei","doi":"10.1007/s00294-021-01222-y","DOIUrl":"https://doi.org/10.1007/s00294-021-01222-y","url":null,"abstract":"Cryptococcus neoformans, basidiomycetous pathogenic yeast, is basically an environmental fungus and, therefore, challenged by ever changing environments. In this study, we focused on how C. neoformans responds to stress caused by cadmium that is one of high-risk pollutants. By tracking phenotypes of the resistance or sensitivity to cadmium, we undertook forward and reverse genetic studies to identify genes involved in cadmium metabolism in C. neoformans. We found that the main route of Cd2+ influx is through Mn2+ ion transporter, Smf1, which is an ortholog of Nramp (natural resistance-associated macrophage protein 1) of mouse. We found that serotype A strains are generally more resistant to cadmium than serotype D strains and that cadmium resistance of H99, a representative of serotype A strains, was found to be due to a partial defect in SMF1. We found that calcium channel has a subsidiary role for cadmium uptake. We also showed that Pca1 (P-type-ATPase) functions as an extrusion pump for cadmium. We examined the effects of some metals on cadmium toxicity and suggested (i) that Ca2+ and Zn2+ could exert their protective function against Cd2+ via restoring cadmium-inhibited cellular processes and (ii) that Mg2+ and Mn2+ could have antagonistic roles in an unknown Smf1-independent Cd2+ uptake system. We proposed a model for Cd2+-response of C. neoformans, which will serve as a platform for understanding how this organism copes with the toxic metal.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":" ","pages":"125-141"},"PeriodicalIF":2.5,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39863314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2