Current Genetics最新文献_第9页

Genetic response to nitrogen starvation in the aggressive Eucalyptus foliar pathogen Teratosphaeria destructans. 桉树叶面致病菌破坏畸胎瘤对氮饥饿的遗传响应。

IF 2.5 4区生物学

Current Genetics Pub Date : 2021-12-01 Epub Date: 2021-08-25 DOI: 10.1007/s00294-021-01208-w

Minette Havenga, Brenda D Wingfield, Michael J Wingfield, Léanne L Dreyer, Francois Roets, Janneke Aylward

{"title":"Genetic response to nitrogen starvation in the aggressive Eucalyptus foliar pathogen Teratosphaeria destructans.","authors":"Minette Havenga, Brenda D Wingfield, Michael J Wingfield, Léanne L Dreyer, Francois Roets, Janneke Aylward","doi":"10.1007/s00294-021-01208-w","DOIUrl":"https://doi.org/10.1007/s00294-021-01208-w","url":null,"abstract":"Teratosphaeria destructans is one of the most aggressive foliar pathogens of Eucalyptus. The biological factors underpinning T. destructans infections, which include shoot and leaf blight on young trees, have never been interrogated. Thus, the means by which the pathogen modifies its host environment to overcome host defences remain unknown. By applying transcriptome sequencing, the aim of this study was to compare gene expression in a South African isolate of T. destructans grown on nitrogen-deficient and complete media. This made it possible to identify upregulated genes in a nitrogen-starved environment, often linked to the pathogenicity of the fungus. The results support the hypothesis that nitrogen starvation in T. destructans likely mirrors an in planta genetic response. This is because 45% of genes that were highly upregulated under nitrogen starvation have previously been reported to be associated with infection in other pathogen systems. These included several CAZymes, fungal effector proteins, peptidases, kinases, toxins, lipases and proteins associated with detoxification of toxic compounds. Twenty-five secondary metabolites were identified and expressed in both nitrogen-deficient and complete conditions. Additionally, the most highly expressed genes in both growth conditions had pathogenicity-related functions. This study highlights the large number of expressed genes associated with pathogenicity and overcoming plant defences. As such, the generated baseline knowledge regarding pathogenicity and aggressiveness in T. destructans is a valuable reference for future in planta work.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"67 6","pages":"981-990"},"PeriodicalIF":2.5,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00294-021-01208-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39343333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Genomic assessment of Stenotrophomonas indicatrix for improved sunflower plant. 改良向日葵窄养单胞菌的基因组鉴定。

IF 2.5 4区生物学

Current Genetics Pub Date : 2021-12-01 Epub Date: 2021-06-30 DOI: 10.1007/s00294-021-01199-8

Bartholomew Saanu Adeleke, Ayansina Segun Ayangbenro, Olubukola Oluranti Babalola

{"title":"Genomic assessment of Stenotrophomonas indicatrix for improved sunflower plant.","authors":"Bartholomew Saanu Adeleke, Ayansina Segun Ayangbenro, Olubukola Oluranti Babalola","doi":"10.1007/s00294-021-01199-8","DOIUrl":"https://doi.org/10.1007/s00294-021-01199-8","url":null,"abstract":"Diverse agriculturally important microbes have been studied with known potential in plant growth promotion. Providing several opportunities, Stenotrophomonas species are characterized as promising plant enhancers, inducers, and protectors against environmental stressors. The S. indicatrix BOVIS40 isolated from the sunflower root endosphere possessed unique features, as genome insights into the Stenotrophomonas species isolated from oilseed crops in Southern Africa have not been reported. Plant growth-promotion screening and genome analysis of S. indicatrix BOVIS40 were presented in this study. The genomic information reveals various genes underlining plant growth promotion and resistance to environmental stressors. The genome of S. indicatrix BOVIS40 harbors genes involved in the degradation and biotransformation of organic molecules. Also, other genes involved in biofilm production, chemotaxis, and flagellation that facilitate bacterial colonization in the root endosphere and phytohormone genes that modulate root development and stress response in plants were detected in strain BOVIS40. IAA activity of the bacterial strain may be a factor responsible for root formation. A measurable approach to the S. indicatrix BOVIS40 lifestyle can strategically provide several opportunities in their use as bioinoculants in developing environmentally friendly agriculture sustainably. The findings presented here provide insights into the genomic functions of S. indicatrix BOVIS40, which has set a foundation for future comparative studies for a better understanding of the synergism among microbes inhabiting plant endosphere. Hence, highlighting the potential of S. indicatrix BOVIS40 upon inoculation under greenhouse experiment, thus suggesting its application in enhancing plant and soil health sustainably.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"67 6","pages":"891-907"},"PeriodicalIF":2.5,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00294-021-01199-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39128236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Genetics animates structure: leveraging genetic interactions to study the dynamics of ribosome biogenesis. 遗传学激活结构:利用遗传相互作用来研究核糖体生物发生的动力学。

IF 2.5 4区生物学

Current Genetics Pub Date : 2021-10-01 DOI: 10.1007/s00294-021-01187-y

Joshua J Black, Arlen W Johnson

{"title":"Genetics animates structure: leveraging genetic interactions to study the dynamics of ribosome biogenesis.","authors":"Joshua J Black, Arlen W Johnson","doi":"10.1007/s00294-021-01187-y","DOIUrl":"https://doi.org/10.1007/s00294-021-01187-y","url":null,"abstract":"The assembly of eukaryotic ribosomes follows an assembly line-like pathway in which numerous trans-acting biogenesis factors act on discrete pre-ribosomal intermediates to progressively shape the nascent subunits into their final functional architecture. Recent advances in cryo-electron microscopy have led to high-resolution structures of many pre-ribosomal intermediates; however, these static snapshots do not capture the dynamic transitions between these intermediates. To this end, molecular genetics can be leveraged to reveal how the biogenesis factors drive these dynamic transitions. Here, we briefly review how we recently used the deletion of BUD23 (bud23∆) to understand its role in the assembly of the ribosomal small subunit. The strong growth defect of bud23∆ mutants places a selective pressure on yeast cells for the occurrence of extragenic suppressors that define a network of functional interactions among biogenesis factors. Mapping these suppressing mutations to recently published structures of pre-ribosomal complexes allowed us to contextualize these suppressing mutations and derive a detailed model in which Bud23 promotes a critical transition event to facilitate folding of the central pseudoknot of the small subunit. This mini-review highlights how genetics can be used to understand the dynamics of complex structures, such as the maturing ribosome.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"67 5","pages":"729-738"},"PeriodicalIF":2.5,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00294-021-01187-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10236300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Single-strand template repair: key insights to increase the efficiency of gene editing. 单链模板修复:提高基因编辑效率的关键见解。

IF 2.5 4区生物学

Current Genetics Pub Date : 2021-10-01 DOI: 10.1007/s00294-021-01186-z

Danielle N Gallagher, James E Haber

引用次数: 9

In memoriam: Stefan Hohmann 纪念:斯特凡·霍曼

IF 2.5 4区生物学

Current Genetics Pub Date : 2021-09-18 DOI: 10.1007/s00294-021-01209-9

S. Hohmann

引用次数: 0

Getting there: understanding the chromosomal recruitment of the AAA+ ATPase Pch2/TRIP13 during meiosis. 到达那里:了解减数分裂期间AAA+ atp酶Pch2/TRIP13的染色体募集。

IF 2.5 4区生物学

Current Genetics Pub Date : 2021-08-01 Epub Date: 2021-03-12 DOI: 10.1007/s00294-021-01166-3

Richard Cardoso da Silva, Gerben Vader

{"title":"Getting there: understanding the chromosomal recruitment of the AAA+ ATPase Pch2/TRIP13 during meiosis.","authors":"Richard Cardoso da Silva, Gerben Vader","doi":"10.1007/s00294-021-01166-3","DOIUrl":"https://doi.org/10.1007/s00294-021-01166-3","url":null,"abstract":"The generally conserved AAA+ ATPase Pch2/TRIP13 is involved in diverse aspects of meiosis, such as prophase checkpoint function, DNA break regulation, and meiotic recombination. The controlled recruitment of Pch2 to meiotic chromosomes allows it to use its ATPase activity to influence HORMA protein-dependent signaling. Because of the connection between Pch2 chromosomal recruitment and its functional roles in meiosis, it is important to reveal the molecular details that govern Pch2 localization. Here, we review the current understanding of the different factors that control the recruitment of Pch2 to meiotic chromosomes, with a focus on research performed in budding yeast. During meiosis in this organism, Pch2 is enriched within the nucleolus, where it likely associates with the specialized chromatin of the ribosomal (r)DNA. Pch2 is also found on non-rDNA euchromatin, where its recruitment is contingent on Zip1, a component of the synaptonemal complex (SC) that assembles between homologous chromosomes. We discuss recent findings connecting the recruitment of Pch2 with its association with the Origin Recognition Complex (ORC) and reliance on RNA Polymerase II-dependent transcription. In total, we provide a comprehensive overview of the pathways that control the chromosomal association of an important meiotic regulator.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"67 4","pages":"553-565"},"PeriodicalIF":2.5,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00294-021-01166-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25470681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Revisiting long-chain fatty acid metabolism in Escherichia coli: integration with stress responses. 重新审视大肠杆菌的长链脂肪酸代谢:与应激反应的整合。

IF 2.5 4区生物学

Current Genetics Pub Date : 2021-08-01 Epub Date: 2021-03-19 DOI: 10.1007/s00294-021-01178-z

Kanchan Jaswal, Megha Shrivastava, Rachna Chaba

{"title":"Revisiting long-chain fatty acid metabolism in Escherichia coli: integration with stress responses.","authors":"Kanchan Jaswal, Megha Shrivastava, Rachna Chaba","doi":"10.1007/s00294-021-01178-z","DOIUrl":"https://doi.org/10.1007/s00294-021-01178-z","url":null,"abstract":"Long-chain fatty acids (LCFAs) are a tremendous source of metabolic energy, an essential component of membranes, and important effector molecules that regulate a myriad of cellular processes. As an energy-rich nutrient source, the role of LCFAs in promoting bacterial survival and infectivity is well appreciated. LCFA degradation generates a large number of reduced cofactors that may confer redox stress; therefore, it is imperative to understand how bacteria deal with this paradoxical situation. Although the LCFA utilization pathway has been studied in great detail, especially in Escherichia coli, where the earliest studies date back to the 1960s, the interconnection of LCFA degradation with bacterial stress responses remained largely unexplored. Recent work in E. coli shows that LCFA degradation induces oxidative stress and also impedes oxidative protein folding. Importantly, both issues arise due to the insufficiency of ubiquinone, a lipid-soluble electron carrier in the electron transport chain. However, to maintain redox homeostasis, bacteria induce sophisticated cellular responses. Here, we review these findings in light of our current knowledge of the LCFA metabolic pathway, metabolism-induced oxidative stress, the process of oxidative protein folding, and stress combat mechanisms. We discuss probable mechanisms for the activation of defense players during LCFA metabolism and the likely feedback imparted by them. We suggest that besides defending against intrinsic stresses, LCFA-mediated upregulation of stress response pathways primes bacteria to adapt to harsh external environments. Collectively, the interplay between LCFA metabolism and stress responses is likely an important factor that underlies the success of LCFA-utilizing bacteria in the host.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"67 4","pages":"573-582"},"PeriodicalIF":2.5,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00294-021-01178-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25495123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The challenges of predicting transposable element activity in hybrids. 预测杂交中转座因子活性的挑战。

IF 2.5 4区生物学

Current Genetics Pub Date : 2021-08-01 Epub Date: 2021-03-18 DOI: 10.1007/s00294-021-01169-0

Mathieu Hénault

引用次数: 5

Mannitol-1-phosphate dehydrogenase, MpdA, is required for mannitol production in vegetative cells and involved in hyphal branching, heat resistance of conidia and sexual development in Aspergillus nidulans. 甘露醇-1-磷酸脱氢酶(MpdA)是营养细胞生产甘露醇所必需的酶，参与了中性曲霉菌丝分支、分生孢子耐热性和性发育。

IF 2.5 4区生物学

Current Genetics Pub Date : 2021-08-01 Epub Date: 2021-03-08 DOI: 10.1007/s00294-021-01163-6

Joo-Yeon Lim, Seung-Hyun Jang, Hee-Moon Park

{"title":"Mannitol-1-phosphate dehydrogenase, MpdA, is required for mannitol production in vegetative cells and involved in hyphal branching, heat resistance of conidia and sexual development in Aspergillus nidulans.","authors":"Joo-Yeon Lim, Seung-Hyun Jang, Hee-Moon Park","doi":"10.1007/s00294-021-01163-6","DOIUrl":"https://doi.org/10.1007/s00294-021-01163-6","url":null,"abstract":"Aspergillus nidulans produces cleistothecia as sexual reproductive organs in a process affected by genetic and external factors. To gain a deeper insight into A. nidulans sexual development, we performed comparative proteome analyses based on the wild type developmental periods. We identified sexual development-specific proteins with a more than twofold increase in production during hypoxia or the sexual period compared to the asexual period. Among the sexual development-specific proteins analyzed by gene-deletion experiments and functional assays, MpdA, a putative mannitol-1-phosphate 5-dehydrogenase, plays multiple roles in growth and differentiation of A. nidulans. The most distinct mpdA-deletion phenotype was ascosporogenesis failure. Genetic mpdA deletion resulted in small cleistothecia with no functional ascospores. Transcriptional analyses indicated that MpdA modulates the expression of key development- and meiosis-regulatory genes during sexual development. The mpdA deletion increased hyphal branching and decreased conidial heat resistance. Mannitol production in conidia showed no difference, whereas it was decreased in mycelia and sexual cultures. Addition of mannitol during vegetative growth recovered the defects in conidial heat resistance and ascospore genesis. Taken together, these results indicate that MpdA plays an important role in sexual development, hyphal branching, and conidial heat resistance in Aspergillus nidulans.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"67 4","pages":"613-630"},"PeriodicalIF":2.5,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00294-021-01163-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25448879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Human OVCA2 and its homolog FSH3-induced apoptosis in Saccharomyces cerevisiae. 人OVCA2及其同源物fsh3诱导酿酒酵母凋亡。

IF 2.5 4区生物学

Current Genetics Pub Date : 2021-08-01 Epub Date: 2021-03-13 DOI: 10.1007/s00294-021-01171-6

Ramachandran Gowsalya, Chidambaram Ravi, Vasanthi Nachiappan

引用次数: 2