Current Genetics最新文献

{"title":"Is heptelidic (koningic) acid a microbial hormone that regulates secondary metabolism in the biocontrol fungus Trichoderma virens?","authors":"Ravindra Bansal, Sunil Kumar Sethy, Vitthal T Barvkar, Prasun Kumar Mukherjee, Suvendu Mondal","doi":"10.1007/s00294-025-01334-9","DOIUrl":"10.1007/s00294-025-01334-9","url":null,"abstract":"<p><p>Trichoderma virens, a plant- beneficial fungus, has the ability to produce volatile and non-volatile secondary metabolites that possess antimicrobial properties and contribute to promoting plant growth and development. Gliovirin, viridin, viridiol, and heptelidic acid are among its major non-volatile metabolites. In our previous study, we observed that the deletion of heptelidic acid synthase (has1), a terpene cyclase, abolished the biosynthesis of major non-volatile metabolites, in addition to heptelidic acid. Conversely, deletion of the Tex7 gene, a non-ribosomal peptide synthetase, led to an increase in heptelidic acid production, along with other major secondary metabolites. Additionally, disruption of a GAPDH gene located within a secondary metabolite gene cluster abolished heptelidic acid biosynthesis and, consequently, all major secondary metabolites. These findings suggest that heptelidic acid might act as a microbial hormone that regulates secondary metabolism. To investigate this further, we conducted transcriptomic and metabolomic comparisons between the wild-type strain and a Δhas1 mutant lacking heptelidic acid biosynthesis. Transcriptome analysis revealed a major proportion of transcripts were down regulated. Of them, nine transcripts belonged to gliovirin biosynthesis cluster, 14 for viridin cluster and five for volatile sesquiterpene and heptelidic acid biosynthesis cluster; metabolite analysis couldn't detect any heptelidic acid along with viridin, viridiol and gliovirin in the Δhas1 mutant. Additionally, several other secondary metabolites were significantly down regulated. Our results are indicative of a possibility that heptelidic acid might function as a microbial hormone regulating secondary metabolism in this biotechnologically important fungus.</p>","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"71 1","pages":"29"},"PeriodicalIF":1.6,"publicationDate":"2025-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12630185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145548348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loss of PDR3 alters metabolome in response to MCHM, a synthetic hydrotrope.","authors":"Taizina Momtareen, Michael C Ayers, Dionysios Patriarcheas, Liam McCarthy, Daniel Judge, Seth Poziviak, Griffen Leombruno, Makaela Quinn, Natalie Wonsettler, Camryn Lowery, Sarah McCulloch, Nathan Dale, Felix Jonas, Jennifer E G Gallagher","doi":"10.1007/s00294-025-01333-w","DOIUrl":"10.1007/s00294-025-01333-w","url":null,"abstract":"<p><p>4-methylcyclohexane methanol (MCHM), an alicyclic alcohol, is a synthetic hydrotrope that induces a starvation response by upregulation of biosynthetic pathways despite the availability of nutrients. To investigate how Saccharomyces cerevisiae adapts its metabolism to tolerate MCHM, we evolved MCHM-resistant strains in In-Lab Evolutions (ILEs). We sequenced the parental (YJM789) with long read sequencing and the evolved strain using short sequencing, increasing the quality of the YJM789 genome. We identified thousands of SNPs and indel variants per ILE strain, which was a consistent number between strains that evolved resistance and control strains that remained sensitive. However, one gene, PDR3, was consistently mutated in all resistant strains. Because it controls the pleiotropic drug response, recurrent mutations in PDR3 across resistant strains indicate that it is a key driver of adaptive resistance to MCHM in yeast. While many of the evolved alleles of PDR3 would likely produce functional proteins, a PDR3 knockout in the parent YJM789 strain was sufficient to reproduce resistance to MCHM. We found that the pdr3 resistance is mediated through Med15, a component of the Mediator complex which regulates activation by mediating interactions between transcription factors of RNA Pol II. Pdr3 can homodimerize or dimerize with Pdr1, another transcription factor paralog and loss of Pdr1 also confers MCHM resistance. The pleiotropic drug response (PDR) pathway facilitates the export of amino acid catabolites, and deletion of PDR3 disrupted glutathione metabolism and its intermediates. Thus, mutations in PDR3 represent the first identified mechanism conferring resistance to this novel hydrotropic chemical.</p>","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"71 1","pages":"30"},"PeriodicalIF":1.9,"publicationDate":"2025-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13332541/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145548425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The CRISPR-cas repertoire of Kluyvera ascorbata: insights from genomic data.","authors":"Çiğdem Yilmaz-Çolak","doi":"10.1007/s00294-025-01332-x","DOIUrl":"10.1007/s00294-025-01332-x","url":null,"abstract":"<p><p>The genus Kluyvera has gained increasing attention due to their emerging role as opportunistic pathogens and their antibiotic resistance determinants. Various approaches have been employed to reveal genomic insights into the evolution and pathogenicity of Kluyvera species. However, detailed knowledge about Kluyvera-specific clustered regularly interspaced short palindromic repeats (CRISPR) is still missing. In this study, a genome-mining approach was employed for the characterization of CRISPR-Cas loci in a total of 13 complete Kluyvera genomes using CRISPRCasFinder and related tools. Out of 13 species, only K. ascorbata displayed multiple CRISPR-Cas arrays and a complete set of cas genes characteristics of a type I-E system. Spacer analysis revealed potential targets within phage and plasmid sequences, indicating historical exposure to mobile genetic elements. Furthermore, a phylogenetic tree constructed using the Cas3 protein sequence positioned K. ascorbata closely with other enteric bacteria, including Salmonella spp. and Citrobacter spp. This study provides the first detailed insight into the CRISPR-Cas architecture of K. ascorbata. Although there is no significant diversity of the CRISPR-Cas system identified in this species, it can emphasize a role as active immune defenses against invaders and offer a foundation for future functional and evolutionary investigations. Moreover, difficulties in identification of the genus Kluyvera can be overcome through the CRISPR-Cas system using next-generation diagnostics tools.</p>","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"71 1","pages":"28"},"PeriodicalIF":1.6,"publicationDate":"2025-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145480936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome characterization of Acinetobacter species from the rice rhizosphere: a potential plant growth promoting rhizobacteria (PGPR).","authors":"Jayateertha R Diwan, Mahadevaswamy, Shivashankaragouda Patil, Divya Bhanu, K Lavanya Devi, Santhosha N Hegde, P U Krishnaraj, R Namitha, Binod B Pradhan, Divya Vashisht","doi":"10.1007/s00294-025-01330-z","DOIUrl":"10.1007/s00294-025-01330-z","url":null,"abstract":"<p><p>Plant-microbe interactions in the rice rhizosphere play a pivotal role for crop productivity. Acinetobacter spp. are known for their plant growth-promoting (PGP) abilities, including phytostimulation, phosphate solubilization, and siderophore production. In this study, we isolated the bacterial strain Acinetobacter lwoffii DJPGP01 from rice rhizosphere soil and conducted comprehensive genome sequencing and analysis. Techniques included serial dilution for bacterial isolation, genomic DNA purification, and Illumina sequencing followed by whole genome data analysis. Genome assembly was performed using multiple assemblers with subsequent annotations through PROKKA and RAST, while pathway analysis utilized KEGG-KAAS server. The genome assembly revealed a high-quality sequence of 3.2 Mb with substantial coverage and a GC content of 43.06%. Species identification confirmed as Acinetobacter lwoffii, closely related to Acinetobacter lwoffii strain DSM 2403 based on 16S rRNA analysis. Antismash analysis identified five secondary metabolite gene clusters, potentially linked to PGP traits. Comparative genome analysis provided insights into the phylogenetic placement of Acinetobacter lwoffii DJPGP01 within its genus, showing a close relationship with Acinetobacter lwoffii NCTC 5866 based on digital DNA-DNA hybridization (DDH). The genome data underlines the potential of Acinetobacter lwoffii as a PGP agent, highlighting specific genes involved in nitrogen assimilation, phosphate uptake, and stress response mechanisms. This study contributes to our understanding of the Acinetobacter role in agricultural settings, suggesting strategies for enhancing crop resilience and productivity.</p>","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"71 1","pages":"27"},"PeriodicalIF":1.9,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145451257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic surveillance of vancomycin-resistant Enterococcus faecium: a study on Resistome, Plasmidome, and mobilome profiling.","authors":"Shalini Mathpal, Avani Panickar, Tushar Joshi, Sudha Ramaiah, Anand Anbarasu","doi":"10.1007/s00294-025-01331-y","DOIUrl":"10.1007/s00294-025-01331-y","url":null,"abstract":"<p><p>Vancomycin-resistant enterococci (VRE) are critical nosocomial pathogens, classified as high priority by the World Health Organization (WHO) due to rising antibiotic resistance. Among these, Vancomycin-resistant Enterococcus faecium (VREfm) presents a significant clinical challenge, frequently detected in healthcare-associated infections and exhibiting resistance to multiple antibiotics. This study presents a genomic surveillance analysis of 63 Enterococcus faecium (E. faecium) isolates obtained from the public database from India during the period January 2017 to December 2021. These isolates were confirmed as VREfm, making them valuable for understanding the key resistance genes and mutations commonly associated with strains. Genomic analysis revealed diverse plasmid replicons such as pRE25, pRUM, and pIP501, often coexisting in single isolates, indicating active horizontal gene transfer. Multiple antimicrobial resistance genes, such as vanHAX, ermB, optrA, and blaOXA-232, were identified along with insertion sequences (IS3, ISL3, IS256), integrons, and transposons (Tn1546, Tn917). Mutations in GyrA, ParC, and PBP5 proteins associated with fluoroquinolone and β-lactam antibiotics were also detected in each isolate. Amino acid substitutions associated with daptomycin resistance were identified in the encoded proteins of the liaR (LiaR-W73C), liaS (LiaS-T120A), cls (Cls-T298S), and rpoB (RpoB-S491F) genes. Three novel deleterious amino acid substitutions were also observed in Cls-R424S, RpoB-M475V, and RpoC-T634K, encoded by the cls, rpoB, and rpoC genes, respectively, that may impact protein function. Overall, this genomic survey provides a framework for hypothesis-driven studies exploring resistance evolution and gene mobility in E. faecium.</p>","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"71 1","pages":"26"},"PeriodicalIF":1.9,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145451301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epigenetic regulation of pathogenicity in fungi.","authors":"Allyson M Hardin, Christina M Hardin, Gary D Isaacs, Michael S Price","doi":"10.1007/s00294-025-01329-6","DOIUrl":"10.1007/s00294-025-01329-6","url":null,"abstract":"<p><p>Histone modifications regulate vital pathogenic features necessary for opportunistic fungi to cause disease. More specifically, histone modification via acetylation and methylation are two major ways that fungi regulate their growth, replication, and ability to respond to stress. This is critically important, as these genes can be potential targets for drug therapies in these difficult-to-treat pathogens. For fungi to rapidly respond to changes in their environment, they must quickly and efficiently adapt to their surroundings. Epigenetic control mechanisms, such as histone modification, allow the fungus to adapt to conditions without altering its DNA. As in other eukaryotes, fungal DNA is tightly packaged into an organized complex known as chromatin and wound around histone proteins. As these histones are modified by methylation or acetylation, specific sections of DNA can become accessible to transcriptional machinery. These histone modifications may interact with various other proteins to accomplish their functions, as well. In this way, histone modifications allow microorganisms to rapidly modify gene expression and respond to their environment. In this review, histone modifications via methylation, demethylation, acetylation, and deacetylation for select model and pathogenic fungal species are summarized concerning how they affect growth, stress response, and gene expression, as applicable. Additionally, the roles of epigenetic regulation in virulence factor expression, toxin formation, and disease are explored. Lastly, current knowledge pertaining to novel drugs and drug targets related to histone acetylation, deacetylation, and demethylation is discussed. Understanding the diverse roles of epigenetic regulation in fungal disease is a crucial first step in exploiting this critical linchpin for novel antifungal treatments of these important human pathogens.</p>","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"71 1","pages":"25"},"PeriodicalIF":1.9,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145344051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic characterization and analysis of Fusarium Solani isolate 1-Jan, a novel pathogenic strain infecting Penaeus vannamei.","authors":"Hui Ge, Qisheng Wu, Yue Ning, Liyun Wu, Huiyao Li, Ping Wen, Xiang Guo, Weigang Wang, Xiaowei Wang, Miaomiao Li, Yanbin Dai, Xiangyang Lin, Qi Lin","doi":"10.1007/s00294-025-01323-y","DOIUrl":"10.1007/s00294-025-01323-y","url":null,"abstract":"<p><p>Fusarium species are pathogens affecting the broodstock of farm-raised Fannabin prawns, resulting in significant economic losses. However, the genomic characteristics and pathogenic mechanisms of these causative Fusarium strains remain incompletely understood. We isolated a novel Fusarium strain, designated 1-Jan, from diseased prawns and confirmed its pathogenicity through artificial infection experiments. Whole-genome sequencing was performed using both PacBio and Illumina platforms. The assembled genome is 51.8 Mb in size, consisting of 54 scaffolds with an N50 length of 1,974 kb. Gene prediction using AUGUSTUS identified 9,769 putative genes. Comparative genomic analysis revealed that strain 1-Jan is closely related to Fusarium solani JS-169 and Nectria haematococca. Candidate pathogenicity genes were identified through annotation against the PHI, FCPD, DFVF, and CAZy databases, yielding 1,101, 126, 479, and 497 hits, respectively. Within the 1-Jan genome, SNP mutations were identified in 737 potential pathogenicity genes, and 859 out of 4,255 single-copy genes were classified as potentially pathogenic. Additionally, 95 of the 967 genes unique to strain 1-Jan were predicted to be involved in pathogenicity. The genome sequence of the 1-Jan strain and the pathogenicity-related genes identified in this study provide valuable insights into the molecular basis of Fusarium-induced diseases in prawns and offer insights for improving the management of fungal infections in aquaculture.</p>","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"71 1","pages":"24"},"PeriodicalIF":1.6,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145279188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial resistance profiles and genomic insights of phenotypically extended spectrum β-lactamase-positive Klebsiella pneumoniae from cattle farms.","authors":"Neha Parmar, Randhir Singh, Anuj Tyagi, Simranpreet Kaur","doi":"10.1007/s00294-025-01326-9","DOIUrl":"10.1007/s00294-025-01326-9","url":null,"abstract":"<p><p>Klebsiella pneumoniae, identified by the World Health Organization (WHO) as a critical priority pathogen, presents a growing public health concern due to increasing multidrug-resistant (MDR) and extended spectrum β-lactamase (ESBL)-producing strains. This study assessed the antimicrobial resistance (AMR) profiles of K. pneumoniae isolates from 288 cattle farm samples in Punjab, India. The bacterium was detected in 10.06% (29/288) of the samples, with 65.51% (19/29) of the isolates being MDR and 6.89% (2/29) exhibiting an ESBL-like phenotype. Resistance genes for β-lactams (bla_TEM-62.06%, bla_AmpC-51.72%, bla_SHV-51.72%), quinolones (qnrS-13.79%), and tetracyclines (tetA-10.34%) were identified. Whole-genome sequencing (WGS) was conducted on two representative phenotypically ESBL-positive and MDR K. pneumoniae isolates, selected for detailed genomic analysis within the limitations of available funding. The analysis revealed the presence of multiple antimicrobial resistance genes (ARGs) conferring resistance to quinolones, cephalosporins, carbapenems, tetracyclines, macrolides, and several other antibiotic classes. Virulence factors detected included Type I and Type III fimbriae, capsules, efflux systems, siderophores, and secretion systems. Plasmid replicon types (IncFIB(K), IncFIB, IncFII(K)), integrons harboring ARGs, and insertion sequences (ISKpn24, ISKpn1, ISKpn19, IS26) were also identified. Multilocus Sequence Typing (MLST) assigned isolates to sequence types ST-160 and ST-4232, with core-genome MLST identifying additional types ST-14,733 and ST-13,365. These findings highlight the potential risk of transmission of resistant K. pneumoniae strains between animals and humans and underscore the urgent need for integrated One Health surveillance and intervention strategies to contain the spread of AMR across sectors.</p>","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"71 1","pages":"23"},"PeriodicalIF":1.6,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyphasic characterization and genomic insights of the cyanobacteria Aphanothece microscopica and A. stagnina from Southern Brazil with emphasis on fatty acid biosynthesis.","authors":"Ronald Tarazona Delgado, Burkhard Becker, Ekaterina Pushkareva, Mathias Schäfer, Carlos Rafael Borges Mendes","doi":"10.1007/s00294-025-01328-7","DOIUrl":"10.1007/s00294-025-01328-7","url":null,"abstract":"<p><p>Cyanobacteria are ecologically pivotal microorganisms with immense biotechnological potential, particularly due to their capacity to synthesize fatty acids, terpenes, and other metabolites with applications ranging from biofuels to nutraceuticals. Despite this, many genera remain genomically underexplored. In this study, we present a polyphasic genomic analysis of two native strains, Aphanothece microscopica RSMan92 and A. stagnina RSMan2012, isolated from the Patos Lagoon estuary in Southern Brazil. Whole-genome sequencing and annotation enabled characterization of both genomes: RSMan92 spans 3.69 Mb in 701 contigs with 3,279 protein-coding sequences, while RSMan2012 comprises 3.28 Mb in 153 contigs with 3,567 protein-coding sequences. Phylogenetic analyses using MUSCLE and RAxML positioned these strains within a well-supported clade closely related to other Aphanothece RefSeq genome, highlighting their evolutionary relatedness and reinforcing the integrative taxonomic placement of the genus. Both strains reveal conserved gene repertoires associated with stress response, fatty acid biosynthesis, and secondary metabolite production (terpenes). Functional classification based on COG and KEGG annotations indicated strong representation of genes involved in lipid metabolism. To investigate how variations in temperature and light intensity modulate metabolite profiles, cultures were subjected to different environmental conditions. GC/EI-MS analysis revealed distinct patterns of fatty acid methyl ester production across conditions: both strains synthesized saturated and monounsaturated fatty acids, whereas only strain RSMan92 exhibited the capacity to synthesize polyunsaturated fatty acids, including linoleic acid derivatives, under variable cultivation conditions. This polyphasic genomic approach, providing novel genomic records, also reveals the fatty acid biosynthetic capacity and metabolic plasticity of Aphanothece strains, emphasizing their biotechnological relevance.</p>","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"71 1","pages":"22"},"PeriodicalIF":1.6,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of intraperitoneal cannabidiol (CBD) injection on intestine microbiome profile in a mouse model.","authors":"Igor Jasielczuk, Ewa Ocłoń, Jakub Żurowski, Tomasz Szmatoła, Karolina Mizera-Szpilka, Artur Gurgul","doi":"10.1007/s00294-025-01327-8","DOIUrl":"10.1007/s00294-025-01327-8","url":null,"abstract":"<p><p>Cannabidiol (CBD) as an active ingredient is widely used in cosmetics, food, medical products, and dietary supplements. Recent studies suggest that CBD consumption may influence the gut microbiome, impacting overall health. The mechanism of CBD action on the intestinal microbiome may be linked to the endocannabinoid system, which plays a role in the normalization of the functions of the immune system. Additionally, CBD may have a direct influence on the gut by interacting with the intestinal microflora, regulating intestinal permeability, modulating immune responses, and affecting the gut-brain axis communication through the vagal signaling pathway. A study was conducted to analyze how different doses of CBD injected intraperitoneal could alter the microbial composition of the duodenum contents in a mouse model. Obtained results suggest that the impact of CBD on the intestine microbiome may be dosage-dependent. Statistical analysis revealed significant differences compared to the control group in the 0.2 mg/kg CBD and 20 mg/kg CBD dose groups for the genera Campilobacteriota, Firmicutes, Proteobacteria, and, specifically in the 0.2 mg CBD group, Cyanobacteria. In contrast, the 10 mg CBD dose group showed no statistically significant differences relative to the control. The Firmicutes phylum had the highest relative abundance in all groups, wherein its share was lower in the CBD-treated groups than in the control group. Lachnospiraceae_NK4A136_group, Muribaculaceae, and Akkermansia were predominant, with Lachnospiraceae_NK4A136_group decreasing and Akkermansia increasing in relative abundance in CBD-treated groups. Of the genera that showed statistically significant differences between groups, most belonged to the phylum Firmicutes, including the dominant Lachnospiraceae_NK4A136_group. These findings suggest that intraperitoneal CBD administration induces alterations in the duodenal microbiome, particularly affecting the Lachnospiraceae_NK4A136_group and the Akkermansia genera.</p>","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"71 1","pages":"21"},"PeriodicalIF":1.9,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12484330/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}