RNA-cleaving DNAzymes as a diagnostic and therapeutic agent against antimicrobial resistant bacteria.

IF 1.8 4区生物学 Q3 GENETICS & HEREDITY

Current Genetics Pub Date : 2022-02-01 Epub Date: 2021-09-09 DOI:10.1007/s00294-021-01212-0

Bao Chi Wong, Juwaini Abu Bakar, Amreeta Dhanoa, Hock Siew Tan

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引用次数: 2

Abstract

The development of nucleic-acid-based antimicrobials such as RNA-cleaving DNAzyme (RCD), a short catalytically active nucleic acid, is a promising alternative to the current antibiotics. The current rapid spread of antimicrobial resistance (AMR) in bacteria renders some antibiotics useless against bacterial infection, thus creating the need for alternative antimicrobials such as DNAzymes. This review summarizes recent advances in the use of RCD as a diagnostic and therapeutic agent against AMR. Firstly, the recent diagnostic application of RCD for the detection of bacterial cells and the associated resistant gene(s) is discussed. The next section summarises the therapeutic application of RCD in AMR bacterial infections which includes direct targeting of the resistant genes and indirect targeting of AMR-associated genes. Finally, this review extends the discussion to challenges of utilizing RCD in real-life applications, and the potential of combining both diagnostic and therapeutic applications of RCD into a single agent as a theranostic agent.

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rna切割DNAzymes作为抗微生物耐药菌的诊断和治疗药物。

基于核酸的抗菌剂的开发，如rna切割核酸(RCD)，是一种具有短催化活性的核酸，是目前抗生素的一个有希望的替代品。目前细菌中抗菌素耐药性(AMR)的迅速传播使得一些抗生素对细菌感染无效，因此需要替代抗菌素，如DNAzymes。本文综述了RCD作为抗抗生素耐药性诊断和治疗药物的最新进展。首先，综述了近年来RCD在细菌细胞及相关耐药基因检测中的诊断应用。下一节总结了RCD在AMR细菌感染中的治疗应用，包括直接靶向耐药基因和间接靶向AMR相关基因。最后，本综述将讨论扩展到RCD在实际应用中的挑战，以及将RCD的诊断和治疗应用结合为一种治疗药物的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Genetics 生物-遗传学

CiteScore

6.00

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Current Genetics publishes genetic, genomic, molecular and systems-level analysis of eukaryotic and prokaryotic microorganisms and cell organelles. All articles are peer-reviewed. The journal welcomes submissions employing any type of research approach, be it analytical (aiming at a better understanding), applied (aiming at practical applications), synthetic or theoretical. Current Genetics no longer accepts manuscripts describing the genome sequence of mitochondria/chloroplast of a small number of species. Manuscripts covering sequence comparisons and analyses that include a large number of species will still be considered.