Current Genetics最新文献_第7页

How adaptive laboratory evolution can boost yeast tolerance to lignocellulosic hydrolyses 适应性实验室进化如何提高酵母对木质纤维素水解物的耐受性

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-04-01 DOI: 10.1007/s00294-022-01237-z

Y. A. Menegon, Jeferson Gross, A. Jacobus

引用次数: 7

Investigating cell autonomy in microorganisms. 研究微生物的细胞自主性。

IF 1.8 4区生物学

Current Genetics Pub Date : 2022-04-01 Epub Date: 2022-02-04 DOI: 10.1007/s00294-022-01231-5

Sarah Piccirillo, Andrew P Morgan, Andy Y Leon, Annika L Smith, Saul M Honigberg

{"title":"Investigating cell autonomy in microorganisms.","authors":"Sarah Piccirillo, Andrew P Morgan, Andy Y Leon, Annika L Smith, Saul M Honigberg","doi":"10.1007/s00294-022-01231-5","DOIUrl":"10.1007/s00294-022-01231-5","url":null,"abstract":"Cell-cell signaling in microorganisms is still poorly characterized. In this Methods paper, we describe a genetic procedure for detecting cell-nonautonomous genetic effects, and in particular cell-cell signaling, termed the chimeric colony assay (CCA). The CCA measures the effect of a gene on a biological response in a neighboring cell. This assay can measure cell autonomy for range of biological activities including transcript or protein accumulation, subcellular localization, and cell differentiation. To date, the CCA has been used exclusively to investigate colony patterning in the budding yeast Saccharomyces cerevisiae. To demonstrate the wider potential of the assay, we applied this assay to two other systems: the effect of Grr1 on glucose repression of GAL1 transcription in yeast and the effect of rpsL on stop-codon translational readthrough in Escherichia coli. We also describe variations of the standard CCA that address specific aspects of cell-cell signaling, and we delineate essential controls for this assay. Finally, we discuss complementary approaches to the CCA. Taken together, this Methods paper demonstrates how genetic assays can reveal and explore the roles of cell-cell signaling in microbial processes.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"68 2","pages":"305-318"},"PeriodicalIF":1.8,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101301/pdf/nihms-1802249.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9595524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of recyclable CRISPR/Cas9 tools for targeted genome editing in the postharvest pathogenic fungi Penicillium digitatum and Penicillium expansum 可回收CRISPR/Cas9工具在采收后致病真菌指状青霉和扩张青霉靶向基因组编辑中的应用

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-03-17 DOI: 10.1007/s00294-022-01236-0

Sandra Garrigues, P. Manzanares, J. Marcos

引用次数: 6

TAL effectors and the predicted host targets of pomegranate bacterial blight pathogen Xanthomonas citri pv. punicae 石榴白叶枯病病原黄单胞菌的TAL效应物和预测宿主靶标。双关语

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-03-11 DOI: 10.1007/s00294-022-01232-4

Sruthi Sivaraman, Dineshkumar Krishnamoorthy, K. Arvind, T. Grace, J. Sharma, Ginny Antony

引用次数: 4

Increased peroxisome proliferation is associated with early yeast replicative ageing 过氧化物酶体增殖增加与酵母菌早期复制老化有关

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-27 DOI: 10.1007/s00294-022-01233-3

Rachayeeta Deb, Suchetana Ghose, Shirisha Nagotu

引用次数: 5

Deciphering β-tubulin gene of carbendazim resistant Fusarium solani isolate and its comparison with other Fusarium species 耐多菌灵番茄枯萎菌分离株β-微管蛋白基因的解析及其与其它枯萎菌的比较

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-15 DOI: 10.1007/s00294-022-01238-y

Mrinmay Tarafder, Bejoysekhar Datta

引用次数: 0

Awakening sleeper cells: a narrative review on bacterial magic spot synthetases as potential drug targets to overcome persistence. 唤醒睡眠细胞:细菌魔点合成酶作为克服持久性的潜在药物靶点的叙述综述。

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-01 DOI: 10.1007/s00294-021-01221-z

Vimal Venu Veetilvalappil, Jesil Mathew Aranjani, Fayaz Shaik Mahammad, Alex Joseph

{"title":"Awakening sleeper cells: a narrative review on bacterial magic spot synthetases as potential drug targets to overcome persistence.","authors":"Vimal Venu Veetilvalappil, Jesil Mathew Aranjani, Fayaz Shaik Mahammad, Alex Joseph","doi":"10.1007/s00294-021-01221-z","DOIUrl":"https://doi.org/10.1007/s00294-021-01221-z","url":null,"abstract":"Magic spot synthetases are emerging targets to overcome persistence caused by stringent response. The 'stringent response' is a bacterial stress survival mechanism, which results in the accumulation of alarmones (also called Magic spots) leading to the formation of dormant persister cells. These 'sleeper cells' evade antibiotic treatment and could result in relapse of infection. This review broadly investigates the phenomenon of stringent response and persistence, and specifically discusses the distribution, classification, and nomenclature of proteins such as Rel/SpoT homologs (RSH), responsible for alarmone synthesis. The authors further explain the relevance of RSH as potential drug targets to break the dormancy of persister cells commonly seen in biofilms. One of the significant factors that initiate alarmone synthesis is nutrient deficiency. In a starved condition, ribosome-associated RSH detects deacylated tRNA and initiates alarmone synthesis. Accumulation of alarmones has a considerable effect on bacterial physiology, virulence, biofilm formation, and persister cell formation. Preventing alarmone synthesis by inhibiting RSH responsible for alarmone synthesis will prevent or reduce persister cells' formation. Magic spot synthetases are thus potential targets that could be explored to overcome persistence seen in biofilms.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"68 1","pages":"49-60"},"PeriodicalIF":2.5,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10317847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Genomic insights into the diversity of non-coding RNAs in Bacillus cereus sensu lato 蜡样芽孢杆菌非编码rna多样性的基因组学研究

IF 2.5 4区生物学

Current Genetics Pub Date : 2021-12-28 DOI: 10.1007/s00294-022-01240-4

Kátia B. Gonçalves, Renan J. Casarotto Appel, L. V. Bôas, P. Cardoso, G. T. V. Bôas

引用次数: 0

Arresting chromosome replication upon energy starvation in Escherichia coli. 在大肠杆菌能量饥饿时阻止染色体复制。

IF 2.5 4区生物学

Current Genetics Pub Date : 2021-12-01 Epub Date: 2021-08-03 DOI: 10.1007/s00294-021-01202-2

Godefroid Charbon, Jakob Frimodt-Møller, Anders Løbner-Olesen

引用次数: 1

HSF1 induces RNA polymerase II synthesis of ribosomal RNA in S. cerevisiae during nitrogen deprivation. 在氮剥夺过程中，HSF1诱导酿酒酵母核糖体RNA的RNA聚合酶II合成。

IF 2.5 4区生物学

Current Genetics Pub Date : 2021-12-01 Epub Date: 2021-08-06 DOI: 10.1007/s00294-021-01197-w

Arjuna Rao Vallabhaneni, Merita Kabashi, Matt Haymowicz, Kushal Bhatt, Violet Wayman, Shazia Ahmed, Heather Conrad-Webb

{"title":"HSF1 induces RNA polymerase II synthesis of ribosomal RNA in S. cerevisiae during nitrogen deprivation.","authors":"Arjuna Rao Vallabhaneni, Merita Kabashi, Matt Haymowicz, Kushal Bhatt, Violet Wayman, Shazia Ahmed, Heather Conrad-Webb","doi":"10.1007/s00294-021-01197-w","DOIUrl":"https://doi.org/10.1007/s00294-021-01197-w","url":null,"abstract":"The resource intensive process of accurate ribosome synthesis is essential for cell viability in all organisms. Ribosome synthesis regulation centers on RNA polymerase I (pol I) transcription of a 35S rRNA precursor that is processed into the mature 18S, 5.8S and 25S rRNAs. During nutrient deprivation or stress, pol I synthesis of rRNA is dramatically reduced. Conversely, chronic stress such as mitochondrial dysfunction induces RNA polymerase II (pol II) to transcribe functional rRNA using an evolutionarily conserved cryptic pol II rDNA promoter suggesting a universal phenomenon. However, this polymerase switches and its role in regulation of rRNA synthesis remain unclear. In this paper, we demonstrate that extended nitrogen deprivation induces the polymerase switch via components of the environmental stress response. We further show that the switch is repressed by Sch9 and activated by the stress kinase Rim15. Like stress-induced genes, the switch requires not only pol II transcription machinery, including the mediator, but also requires the HDAC, Rpd3 and stress transcription factor Hsf1. The current work shows that the constitutive allele, Hsf1PO4* displays elevated levels of induction in non-stress conditions while binding to a conserved site in the pol II rDNA promoter upstream of the pol I promoter. Whether the polymerase switch serves to provide rRNA when pol I transcription is inhibited or fine-tunes pol I initiation via RNA interactions is yet to be determined. Identifying the underlying mechanism for this evolutionary conserved phenomenon will help understand the mechanism of pol II rRNA synthesis and its role in stress adaptation.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"67 6","pages":"937-951"},"PeriodicalIF":2.5,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39297006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1