Current Genetics最新文献_第7页

The antifungal effect induced by itraconazole in Candida parapsilosis largely depends on the oxidative stress generated at the mitochondria. 伊曲康唑对假丝酵母的抗真菌作用很大程度上取决于线粒体产生的氧化应激。

IF 2.5 4区生物学

Current Genetics Pub Date : 2023-06-01 DOI: 10.1007/s00294-023-01269-z

Mª Luz Muñoz-Megías, Ruth Sánchez-Fresneda, Francisco Solano, Sergi Maicas, María Martínez-Esparza, Juan-Carlos Argüelles

{"title":"The antifungal effect induced by itraconazole in Candida parapsilosis largely depends on the oxidative stress generated at the mitochondria.","authors":"Mª Luz Muñoz-Megías, Ruth Sánchez-Fresneda, Francisco Solano, Sergi Maicas, María Martínez-Esparza, Juan-Carlos Argüelles","doi":"10.1007/s00294-023-01269-z","DOIUrl":"https://doi.org/10.1007/s00294-023-01269-z","url":null,"abstract":"In Candida parapsilosis, homozygous disruption of the two genes encoding trehalase activity increased the susceptibility to Itraconazole compared with the isogenic parental strain. The fungicidal effect of this azole can largely be counteracted by preincubating growing cells with rotenone and the protonophore 2,4-Dinitrophenol. In turn, measurement of endogenous reactive oxygen species formation by flow cytometry confirmed that Itraconazole clearly induced an internal oxidative stress, which can be significantly abolished in rotenone-exposed cells. Analysis of the antioxidant enzymatic activities of catalase and superoxide dismutase pointed to a moderate decrease of catalase in trehalase-deficient mutant cells compared to the wild type, with an additional increase upon addition of rotenone. These enzymatic changes were imperceptible in the case of superoxide dismutase. Alternative assays with Voriconazole led to a similar profile in the results regarding cell growth and antioxidant activities. Collectively, our data suggest that the antifungal action of Itraconazole on C. parapsilosis is dependent on a functional mitochondrial activity. They also suggest that the central metabolic pathways in pathogenic fungi should be considered as preferential antifungal targets in new research.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"69 2-3","pages":"165-173"},"PeriodicalIF":2.5,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10163099/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10039597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The N-terminal disordered region of ChsB regulates its efficient transport to the hyphal apical surface in Aspergillus nidulans. 在芽曲霉中，ChsB的n端紊乱区调节其向菌丝顶端表面的有效运输。

IF 2.5 4区生物学

Current Genetics Pub Date : 2023-06-01 DOI: 10.1007/s00294-023-01267-1

Jingyun Jin, Ryo Iwama, Hiroyuki Horiuchi

{"title":"The N-terminal disordered region of ChsB regulates its efficient transport to the hyphal apical surface in Aspergillus nidulans.","authors":"Jingyun Jin, Ryo Iwama, Hiroyuki Horiuchi","doi":"10.1007/s00294-023-01267-1","DOIUrl":"https://doi.org/10.1007/s00294-023-01267-1","url":null,"abstract":"In fungi, the cell wall plays a crucial role in morphogenesis and response to stress from the external environment. Chitin is one of the main cell wall components in many filamentous fungi. In Aspergillus nidulans, a class III chitin synthase ChsB plays a pivotal role in hyphal extension and morphogenesis. However, little is known about post-translational modifications of ChsB and their functional impacts. In this study, we showed that ChsB is phosphorylated in vivo. We characterized strains that produce ChsB using stepwise truncations of its N-terminal disordered region or deletions of some residues in that region and demonstrated its involvement in ChsB abundance on the hyphal apical surface and in hyphal tip localization. Furthermore, we showed that some deletions in this region affected the phosphorylation states of ChsB, raising the possibility that these states are important for the localization of ChsB to the hyphal surface and the growth of A. nidulans. Our findings indicate that ChsB transport is regulated by its N-terminal disordered region.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"69 2-3","pages":"175-188"},"PeriodicalIF":2.5,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10163080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9680838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tfs1, transcription elongation factor TFIIS, has an impact on chromosome segregation affected by pka1 deletion in Schizosaccharomyces pombe. 转录延伸因子TFIIS对裂糖酵母pka1缺失对染色体分离的影响。

IF 1.9 4区生物学

Current Genetics Pub Date : 2023-06-01 Epub Date: 2023-04-13 DOI: 10.1007/s00294-023-01268-0

Kouhei Takenaka, Shiho Nishioka, Yuki Nishida, Makoto Kawamukai, Yasuhiro Matsuo

{"title":"Tfs1, transcription elongation factor TFIIS, has an impact on chromosome segregation affected by pka1 deletion in Schizosaccharomyces pombe.","authors":"Kouhei Takenaka, Shiho Nishioka, Yuki Nishida, Makoto Kawamukai, Yasuhiro Matsuo","doi":"10.1007/s00294-023-01268-0","DOIUrl":"10.1007/s00294-023-01268-0","url":null,"abstract":"The cAMP-dependent protein kinase (PKA) pathway in Schizosaccharomyces pombe plays an important role in microtubule organization and chromosome segregation. Typically, loss of functional Pka1 induces sensitivity to the microtubule-destabilizing drug thiabendazole (TBZ) and chromosome mis-segregation. To determine the mechanism via which Pka1 is involved in these events, we explored the relevance of transcription factors by creating a double-deletion strain of pka1 and 102 individual genes encoding transcription factors. We found that rst2∆, tfs1∆, mca1∆, and moc3∆ suppressed the TBZ-sensitive phenotype of the pka1∆ strain, among which tfs1∆ was the strongest suppressor. All single mutants (rst2∆, tfs1∆, mca1∆, and moc3∆) showed a TBZ-tolerant phenotype. Tfs1 has two transcriptional domains (TFIIS and Zn finger domains), both of which contributed to the suppression of the pka1∆-induced TBZ-sensitive phenotype. pka1∆-induced chromosome mis-segregation was rescued by tfs1∆ in the presence of TBZ. tfs1 overexpression induced the TBZ-sensitive phenotype and a high frequency of chromosome mis-segregation, suggesting that the amount of Tfs1 must be strictly controlled. However, Tfs1-expression levels did not differ between the wild-type and pka1∆ strains, and the Tfs1-GFP protein was localized to the nucleus and cytoplasm in both strains, which excludes the direct regulation of expression and localization of Tfs1 by Pka1. Growth inhibition by TBZ in pka1∆ strains was notably rescued by double deletion of rst2 and tfs1 rather than single deletion of rst2 or tfs1, indicating that Rst2 and Tfs1 contribute independently to counteract TBZ toxicity. Our findings highlight Tfs1 as a key transcription factor for proper chromosome segregation.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"69 2-3","pages":"115-125"},"PeriodicalIF":1.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9420607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional characterization and comparative analysis of gene repression-mediating domains interacting with yeast pleiotropic corepressors Sin3, Cyc8 and Tup1. 酵母多效性共抑制因子Sin3、Cyc8和Tup1相互作用的基因抑制介导域功能表征及比较分析。

IF 2.5 4区生物学

Current Genetics Pub Date : 2023-06-01 DOI: 10.1007/s00294-023-01262-6

Julia Lettow, Felix Kliewe, Rasha Aref, Hans-Joachim Schüller

{"title":"Functional characterization and comparative analysis of gene repression-mediating domains interacting with yeast pleiotropic corepressors Sin3, Cyc8 and Tup1.","authors":"Julia Lettow, Felix Kliewe, Rasha Aref, Hans-Joachim Schüller","doi":"10.1007/s00294-023-01262-6","DOIUrl":"https://doi.org/10.1007/s00294-023-01262-6","url":null,"abstract":"Transcriptional corepressors Sin3, Cyc8 and Tup1 are important for downregulation of gene expression by recruiting various histone deacetylases once they gain access to defined genomic locations by interaction with pathway-specific repressor proteins. In this work we systematically investigated whether 17 yeast repressor proteins (Cti6, Dal80, Fkh1, Gal80, Mig1, Mot3, Nrg1, Opi1, Rdr1, Rox1, Sko1, Ume6, Ure2, Xbp1, Yhp1, Yox1 and Whi5) representing several unrelated regulatory pathways are able to bind to Sin3, Cyc8 and Tup1. Our results show that paired amphipathic helices 1 and 2 (PAH1 and PAH2) of Sin3 are functionally redundant for some regulatory pathways. WD40 domains of Tup1 proved to be sufficient for interaction with repressor proteins. Using length variants of selected repressors, we mapped corepressor interaction domains (CIDs) in vitro and assayed gene repression in vivo. Systematic comparison of CID minimal sequences allowed us to define several related positional patterns of hydrophobic amino acids some of which could be confirmed as functionally supported by site-directed mutagenesis. Although structural predictions indicated that certain CIDs may be α-helical, most repression domains appear to be randomly structured and must be considered as intrinsically disordered regions (IDR) adopting a defined conformation only by interaction with a corepressor.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"69 2-3","pages":"127-139"},"PeriodicalIF":2.5,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10163088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9682479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Diverse and dynamic forms of gene regulation by the S. cerevisiae histone methyltransferase Set1. 麦角菌组蛋白甲基转移酶 Set1 对基因调控的多样动态形式

IF 1.9 4区生物学

Current Genetics Pub Date : 2023-06-01 Epub Date: 2023-03-31 DOI: 10.1007/s00294-023-01265-3

Neha Deshpande, Mary Bryk

{"title":"Diverse and dynamic forms of gene regulation by the S. cerevisiae histone methyltransferase Set1.","authors":"Neha Deshpande, Mary Bryk","doi":"10.1007/s00294-023-01265-3","DOIUrl":"10.1007/s00294-023-01265-3","url":null,"abstract":"Gene transcription is an essential and highly regulated process. In eukaryotic cells, the structural organization of nucleosomes with DNA wrapped around histone proteins impedes transcription. Chromatin remodelers, transcription factors, co-activators, and histone-modifying enzymes work together to make DNA accessible to RNA polymerase. Histone lysine methylation can positively or negatively regulate gene transcription. Methylation of histone 3 lysine 4 by SET-domain-containing proteins is evolutionarily conserved from yeast to humans. In higher eukaryotes, mutations in SET-domain proteins are associated with defects in the development and segmentation of embryos, skeletal and muscle development, and diseases, including several leukemias. Since histone methyltransferases are evolutionarily conserved, the mechanisms of gene regulation mediated by these enzymes are also conserved. Budding yeast Saccharomyces cerevisiae is an excellent model system to study the impact of histone 3 lysine 4 (H3K4) methylation on eukaryotic gene regulation. Unlike larger eukaryotes, yeast cells have only one enzyme that catalyzes H3K4 methylation, Set1. In this review, we summarize current knowledge about the impact of Set1-catalyzed H3K4 methylation on gene transcription in S. cerevisiae. We describe the COMPASS complex, factors that influence H3K4 methylation, and the roles of Set1 in gene silencing at telomeres and heterochromatin, as well as repression and activation at euchromatic loci. We also discuss proteins that \"read\" H3K4 methyl marks to regulate transcription and summarize alternate functions for Set1 beyond H3K4 methylation.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"69 2-3","pages":"91-114"},"PeriodicalIF":1.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9442451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphogenic plasticity: the pathogenic attribute of Candida albicans. 形态发生可塑性：白色念珠菌的致病特性。

IF 1.9 4区生物学

Current Genetics Pub Date : 2023-06-01 Epub Date: 2023-03-22 DOI: 10.1007/s00294-023-01263-5

Priya Prasad, Meena Tippana

引用次数: 0

Genomic analyses of drug-resistant Salmonella enterica serovar Heidelberg strains isolated from meat and related sources between 2013 and 2017 in the south region of Brazil. 2013年至2017年在巴西南部地区从肉类和相关来源分离的耐药肠沙门氏菌血清型海德堡菌株的基因组分析。

IF 1.9 4区生物学

Current Genetics Pub Date : 2023-06-01 Epub Date: 2023-03-15 DOI: 10.1007/s00294-023-01264-4

Felipe Pinheiro Vilela, Dália Dos Prazeres Rodrigues, Marc William Allard, Juliana Pfrimer Falcão

{"title":"Genomic analyses of drug-resistant Salmonella enterica serovar Heidelberg strains isolated from meat and related sources between 2013 and 2017 in the south region of Brazil.","authors":"Felipe Pinheiro Vilela, Dália Dos Prazeres Rodrigues, Marc William Allard, Juliana Pfrimer Falcão","doi":"10.1007/s00294-023-01264-4","DOIUrl":"10.1007/s00294-023-01264-4","url":null,"abstract":"Salmonella enterica serovar Heidelberg (S. Heidelberg) is a zoonotic, ubiquitous, and worldwide-distributed pathogen, responsible for gastroenteritis in humans caused by the consumption of contaminated food. In this study, 11 S. Heidelberg strains isolated from chicken and bovine meat, drag swab, and animal feed between 2013 and 2017 in states of the southern region of Brazil were characterized by whole-genome sequencing (WGS) analyses. Antimicrobial resistance against 18 antimicrobials was determined by disk-diffusion and ciprofloxacin's minimum inhibitory concentration by Etest®. The search for resistance and virulence genes, plasmids, Salmonella Pathogenicity Islands (SPIs) plus multi-locus sequence typing (MLST), and single-nucleotide polymorphisms (SNPs) analyses was conducted using WGS data. All strains harbored resistance genes fosA7, aac(6')-Iaa, sul2, tet(A), blaCMY-2, mdsA, and mdsB, and point mutations in gyrA and parC. All strains showed a phenotypic multidrug-resistant profile, with resistant or intermediate resistant profiles against 14 antimicrobials tested. Plasmids ColpVC, IncC, IncX1, and IncI1-I(Alpha) were detected. Virulence genes related to adherence, macrophage induction, magnesium uptake, regulation, and type III secretion systems plus 10 SPIs were detected. All strains were assigned to ST15 and belonged to two SNP clusters showing high similarity to isolates from the United Kingdom, Chile, Germany, the Netherlands, China, South Africa, and South Korea. In conclusion, the presence of multidrug-resistant S. Heidelberg strains in Brazil showing a global genomic relationship may alert for the necessity of stronger surveillance measures by food safety and public health authorities to limit its spread to humans and animals through foods.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"69 2-3","pages":"141-152"},"PeriodicalIF":1.9,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9419640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of nanotags and nanobodies for live cell single-molecule imaging of the Z-ring in Escherichia coli. 应用纳米标签和纳米抗体对大肠杆菌的 Z 环进行活细胞单分子成像。

IF 1.8 4区生物学

Current Genetics Pub Date : 2023-06-01 Epub Date: 2023-04-06 DOI: 10.1007/s00294-023-01266-2

Emma Westlund, Axel Bergenstråle, Alaska Pokhrel, Helena Chan, Ulf Skoglund, Daniel O Daley, Bill Söderström

引用次数: 0

VdERG2 was involved in ergosterol biosynthesis, nutritional differentiation and virulence of Verticillium dahliae. VdERG2参与了大丽花黄萎病麦角甾醇的生物合成、营养分化和毒力过程。

IF 1.9 4区生物学

Current Genetics Pub Date : 2023-02-01 Epub Date: 2022-11-22 DOI: 10.1007/s00294-022-01257-9

Junyuan Lv, Shichao Liu, Xiaojian Zhang, Lihong Zhao, Tao Zhang, Zhigang Zhang, Zili Feng, Feng Wei, Jinglong Zhou, Ruiyuan Zhao, Hongjie Feng, Heqin Zhu, Caihong Li, Yalin Zhang

{"title":"VdERG2 was involved in ergosterol biosynthesis, nutritional differentiation and virulence of Verticillium dahliae.","authors":"Junyuan Lv, Shichao Liu, Xiaojian Zhang, Lihong Zhao, Tao Zhang, Zhigang Zhang, Zili Feng, Feng Wei, Jinglong Zhou, Ruiyuan Zhao, Hongjie Feng, Heqin Zhu, Caihong Li, Yalin Zhang","doi":"10.1007/s00294-022-01257-9","DOIUrl":"10.1007/s00294-022-01257-9","url":null,"abstract":"The ergosterol biosynthesis pathway plays an important role in model pathogenic bacteria Saccharomyces cerevisiae, but little is known about the biosynthesis of ergosterol in the pathogenic fungus Verticillium dahliae. In this study, we identified the VdERG2 gene encoding sterol C-8 isomerase from V. dahliae and investigated its function in virulence by generating gene deletion mutants (ΔVdERG2) and complemented mutants (C-ΔVdERG2). Knockout of VdERG2 reduced ergosterol content. The conidial germination rate and conidial yield of ΔVdERG2 significantly decreased and abnormal conidia were produced. In spite of VdERG2 did not affect the utilization of carbon sources by V. dahliae, but the melanin production of ΔVdERG2 was decreased in cellulose and pectin were used as the sole carbon sources. Furthermore, the ΔVdERG2 mutants produced less microsclerotia and melanin with a significant decrease in the expression of microsclerotia and melanin-related genes VaflM, Vayg1, VDH1, VdLAC, VdSCD and VT4HR. In addition, mutants ΔVdERG2 were very sensitive to congo red (CR), sodium dodecyl sulfate (SDS) and hydrogen peroxide (H2O2) stresses, indicating that VdERG2 was involved in the cell wall and oxidative stress response. The absence of VdERG2 weakened the penetration ability of mycelium on cellophane and affected the growth of mycelium. Although ΔVdERG2 could infect cotton, its pathogenicity was significantly impaired. These phenotypic defects in ΔVdERG2 could be complemented by the reintroduction of a full-length VdERG2 gene. In summary, as a single conservative secretory protein, VdERG2 played a crucial role in ergosterol biosynthesis, nutritional differentiation and virulence in V. dahliae.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"69 1","pages":"25-40"},"PeriodicalIF":1.9,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10702501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondria in Cryptococcus: an update of mitochondrial transcriptional regulation in Cryptococcus. 隐球菌线粒体：隐球菌线粒体转录调控的最新进展。

IF 1.9 4区生物学

Current Genetics Pub Date : 2023-02-01 Epub Date: 2023-02-02 DOI: 10.1007/s00294-023-01261-7

Yang Meng, Chen Ding

引用次数: 0