Current Genetics最新文献_第7页

Increased peroxisome proliferation is associated with early yeast replicative ageing 过氧化物酶体增殖增加与酵母菌早期复制老化有关

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-27 DOI: 10.1007/s00294-022-01233-3

Rachayeeta Deb, Suchetana Ghose, Shirisha Nagotu

引用次数: 5

Deciphering β-tubulin gene of carbendazim resistant Fusarium solani isolate and its comparison with other Fusarium species 耐多菌灵番茄枯萎菌分离株β-微管蛋白基因的解析及其与其它枯萎菌的比较

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-15 DOI: 10.1007/s00294-022-01238-y

Mrinmay Tarafder, Bejoysekhar Datta

引用次数: 0

Awakening sleeper cells: a narrative review on bacterial magic spot synthetases as potential drug targets to overcome persistence. 唤醒睡眠细胞:细菌魔点合成酶作为克服持久性的潜在药物靶点的叙述综述。

IF 2.5 4区生物学

Current Genetics Pub Date : 2022-02-01 DOI: 10.1007/s00294-021-01221-z

Vimal Venu Veetilvalappil, Jesil Mathew Aranjani, Fayaz Shaik Mahammad, Alex Joseph

{"title":"Awakening sleeper cells: a narrative review on bacterial magic spot synthetases as potential drug targets to overcome persistence.","authors":"Vimal Venu Veetilvalappil, Jesil Mathew Aranjani, Fayaz Shaik Mahammad, Alex Joseph","doi":"10.1007/s00294-021-01221-z","DOIUrl":"https://doi.org/10.1007/s00294-021-01221-z","url":null,"abstract":"Magic spot synthetases are emerging targets to overcome persistence caused by stringent response. The 'stringent response' is a bacterial stress survival mechanism, which results in the accumulation of alarmones (also called Magic spots) leading to the formation of dormant persister cells. These 'sleeper cells' evade antibiotic treatment and could result in relapse of infection. This review broadly investigates the phenomenon of stringent response and persistence, and specifically discusses the distribution, classification, and nomenclature of proteins such as Rel/SpoT homologs (RSH), responsible for alarmone synthesis. The authors further explain the relevance of RSH as potential drug targets to break the dormancy of persister cells commonly seen in biofilms. One of the significant factors that initiate alarmone synthesis is nutrient deficiency. In a starved condition, ribosome-associated RSH detects deacylated tRNA and initiates alarmone synthesis. Accumulation of alarmones has a considerable effect on bacterial physiology, virulence, biofilm formation, and persister cell formation. Preventing alarmone synthesis by inhibiting RSH responsible for alarmone synthesis will prevent or reduce persister cells' formation. Magic spot synthetases are thus potential targets that could be explored to overcome persistence seen in biofilms.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"68 1","pages":"49-60"},"PeriodicalIF":2.5,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10317847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Genomic insights into the diversity of non-coding RNAs in Bacillus cereus sensu lato 蜡样芽孢杆菌非编码rna多样性的基因组学研究

IF 2.5 4区生物学

Current Genetics Pub Date : 2021-12-28 DOI: 10.1007/s00294-022-01240-4

Kátia B. Gonçalves, Renan J. Casarotto Appel, L. V. Bôas, P. Cardoso, G. T. V. Bôas

引用次数: 0

Arresting chromosome replication upon energy starvation in Escherichia coli. 在大肠杆菌能量饥饿时阻止染色体复制。

IF 2.5 4区生物学

Current Genetics Pub Date : 2021-12-01 Epub Date: 2021-08-03 DOI: 10.1007/s00294-021-01202-2

Godefroid Charbon, Jakob Frimodt-Møller, Anders Løbner-Olesen

引用次数: 1

HSF1 induces RNA polymerase II synthesis of ribosomal RNA in S. cerevisiae during nitrogen deprivation. 在氮剥夺过程中，HSF1诱导酿酒酵母核糖体RNA的RNA聚合酶II合成。

IF 2.5 4区生物学

Current Genetics Pub Date : 2021-12-01 Epub Date: 2021-08-06 DOI: 10.1007/s00294-021-01197-w

Arjuna Rao Vallabhaneni, Merita Kabashi, Matt Haymowicz, Kushal Bhatt, Violet Wayman, Shazia Ahmed, Heather Conrad-Webb

{"title":"HSF1 induces RNA polymerase II synthesis of ribosomal RNA in S. cerevisiae during nitrogen deprivation.","authors":"Arjuna Rao Vallabhaneni, Merita Kabashi, Matt Haymowicz, Kushal Bhatt, Violet Wayman, Shazia Ahmed, Heather Conrad-Webb","doi":"10.1007/s00294-021-01197-w","DOIUrl":"https://doi.org/10.1007/s00294-021-01197-w","url":null,"abstract":"The resource intensive process of accurate ribosome synthesis is essential for cell viability in all organisms. Ribosome synthesis regulation centers on RNA polymerase I (pol I) transcription of a 35S rRNA precursor that is processed into the mature 18S, 5.8S and 25S rRNAs. During nutrient deprivation or stress, pol I synthesis of rRNA is dramatically reduced. Conversely, chronic stress such as mitochondrial dysfunction induces RNA polymerase II (pol II) to transcribe functional rRNA using an evolutionarily conserved cryptic pol II rDNA promoter suggesting a universal phenomenon. However, this polymerase switches and its role in regulation of rRNA synthesis remain unclear. In this paper, we demonstrate that extended nitrogen deprivation induces the polymerase switch via components of the environmental stress response. We further show that the switch is repressed by Sch9 and activated by the stress kinase Rim15. Like stress-induced genes, the switch requires not only pol II transcription machinery, including the mediator, but also requires the HDAC, Rpd3 and stress transcription factor Hsf1. The current work shows that the constitutive allele, Hsf1PO4* displays elevated levels of induction in non-stress conditions while binding to a conserved site in the pol II rDNA promoter upstream of the pol I promoter. Whether the polymerase switch serves to provide rRNA when pol I transcription is inhibited or fine-tunes pol I initiation via RNA interactions is yet to be determined. Identifying the underlying mechanism for this evolutionary conserved phenomenon will help understand the mechanism of pol II rRNA synthesis and its role in stress adaptation.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"67 6","pages":"937-951"},"PeriodicalIF":2.5,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39297006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Contribution of SOS genes to H₂O₂-induced apoptosis-like death in Escherichia coli. SOS基因在h2o2诱导的大肠杆菌细胞凋亡样死亡中的作用。

IF 2.5 4区生物学

Current Genetics Pub Date : 2021-12-01 Epub Date: 2021-08-25 DOI: 10.1007/s00294-021-01204-0

Heesu Kim, Dong Gun Lee

{"title":"Contribution of SOS genes to H2O2-induced apoptosis-like death in Escherichia coli.","authors":"Heesu Kim, Dong Gun Lee","doi":"10.1007/s00294-021-01204-0","DOIUrl":"https://doi.org/10.1007/s00294-021-01204-0","url":null,"abstract":"Hydrogen peroxide (H2O2) is a debriding agent that damages the microbial structure and function by generating various reactive oxygen species (ROS). H2O2-produced hydroxyl radical (OH∙) also exerts oxidative stress on microorganisms. The spread of antibiotic-resistance in bacteria is a serious issue worldwide, and greater efforts are needed to identify and characterize novel antibacterial mechanisms to develop new treatment strategies. Therefore, this study aimed to clarify the relationship between H2O2 and Escherichia coli and to elucidate a novel antibacterial mechanism(s) of H2O2. Following H2O2 exposure, increased levels of 8-hydroxydeoxyguanosine and malondialdehyde indicated that H2O2 accelerates oxidation of bacterial DNA and lipids in E. coli. As oxidative damage worsened, the SOS response was triggered. Cell division arrest and resulting filamentous cells were identified in cells, indicating that LexA was involved in DNA replication. It was also verified that RecA, a representative SOS gene, helps self-cleavage of LexA and acts as a bacterial caspase-like protein. Our findings also showed that dinF is essential to preserve E. coli from H2O2-induced ROS, and furthermore, demonstrated that H2O2-induced SOS response and SOS genes participate differently in guarding E. coli from oxidative stress. As an extreme SOS response is considered apoptosis-like death (ALD) in bacteria, additional experiments were performed to examine the characteristics of ALD. DNA fragmentation and membrane depolarization appeared in H2O2-treated cells, suggesting that H2O2 causes ALD in E. coli. In conclusion, our investigations revealed that ALD is a novel antibacterial mode of action(s) of H2O2 with important contributions from SOS genes.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"67 6","pages":"969-980"},"PeriodicalIF":2.5,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00294-021-01204-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39359784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Homology length dictates the requirement for Rad51 and Rad52 in gene targeting in the Basidiomycota yeast Naganishia liquefaciens. 同源长度决定了长岛液化担子菌对Rad51和Rad52基因定位的要求。

IF 2.5 4区生物学

Current Genetics Pub Date : 2021-12-01 Epub Date: 2021-07-22 DOI: 10.1007/s00294-021-01201-3

Maierdan Palihati, Hideo Tsubouchi, Bilge Argunhan, Rei Kajitani, Omirgul Bakenova, Yong-Woon Han, Yasuto Murayama, Takehiko Itoh, Hiroshi Iwasaki

{"title":"Homology length dictates the requirement for Rad51 and Rad52 in gene targeting in the Basidiomycota yeast Naganishia liquefaciens.","authors":"Maierdan Palihati, Hideo Tsubouchi, Bilge Argunhan, Rei Kajitani, Omirgul Bakenova, Yong-Woon Han, Yasuto Murayama, Takehiko Itoh, Hiroshi Iwasaki","doi":"10.1007/s00294-021-01201-3","DOIUrl":"https://doi.org/10.1007/s00294-021-01201-3","url":null,"abstract":"Here, we report the development of methodologies that enable genetic modification of a Basidiomycota yeast, Naganishia liquifaciens. The gene targeting method employs electroporation with PCR products flanked by an 80 bp sequence homologous to the target. The method, combined with a newly devised CRISPR-Cas9 system, routinely achieves 80% gene targeting efficiency. We further explored the genetic requirement for this homologous recombination (HR)-mediated gene targeting. The absence of Ku70, a major component of the non-homologous end joining (NHEJ) pathway of DNA double-strand break repair, almost completely eliminated inaccurate integration of the marker. Gene targeting with short homology (80 bp) was almost exclusively dependent on Rad52, an essential component of HR in the Ascomycota yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. By contrast, the RecA homolog Rad51, which performs homology search and strand exchange in HR, plays a relatively minor role in gene targeting, regardless of the homology length (80 bp or 1 kb). The absence of both Rad51 and Rad52, however, completely eliminated gene targeting. Unlike Ascomycota yeasts, the absence of Rad52 in N. liquefaciens conferred only mild sensitivity to ionizing radiation. These traits associated with the absence of Rad52 are reminiscent of findings in mice.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"67 6","pages":"919-936"},"PeriodicalIF":2.5,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00294-021-01201-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39211579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Reversible protein aggregation as cytoprotective mechanism against heat stress. 可逆蛋白聚集作为热应激的细胞保护机制。

IF 2.5 4区生物学

Current Genetics Pub Date : 2021-12-01 Epub Date: 2021-06-06 DOI: 10.1007/s00294-021-01191-2

Paola Gallardo, Silvia Salas-Pino, Rafael R Daga

引用次数: 6

Stress granules safeguard against MAPK signaling hyperactivation by sequestering PKC/Pck2: new findings and perspectives. 应激颗粒通过隔离PKC/Pck2来防止MAPK信号过度激活:新发现和新观点。

IF 2.5 4区生物学

Current Genetics Pub Date : 2021-12-01 Epub Date: 2021-06-07 DOI: 10.1007/s00294-021-01192-1

Reiko Sugiura

{"title":"Stress granules safeguard against MAPK signaling hyperactivation by sequestering PKC/Pck2: new findings and perspectives.","authors":"Reiko Sugiura","doi":"10.1007/s00294-021-01192-1","DOIUrl":"https://doi.org/10.1007/s00294-021-01192-1","url":null,"abstract":"Stress granule (SG) assembly is a conserved cellular strategy that copes with stress-related damage and promotes cell survival. SGs form through a process of liquid-liquid phase separation. Cellular signaling also appears to employ SG assembly as a mechanism for controlling cell survival and cell death by spatial compartmentalization of signal-transducing factors. While several lines of evidence highlight the importance of SGs as signaling hubs, where protein components of signaling pathways can be temporarily sequestered, shielded from the cytoplasm, the regulation and physiological significance of SGs in this aspect remain largely obscure. A recent study of the heat-shock response in the fission yeast Schizosaaccharomyces pombe provides an unexpected answer to this question. Recently, we demonstrated that the PKC orthologue Pck2 in fission yeast translocates into SGs through phase separation in a PKC kinase activity-dependent manner upon high-heat stress (HHS). Importantly, the downstream MAPK Pmk1 promotes Pck2 recruitment into SGs, which intercepts MAPK hyperactivation and cell death, thus posing SGs as a negative feedback circuit in controlling MAPK signaling. Intriguingly, HHS, but not modest-heat stress targets Pck2 to SGs, independent of canonical SG machinery. Finally, cells fail to activate MAPK signaling when Pck2 is sequestrated into SGs. In this review, we will discuss how SGs have a role as signaling hubs beyond serving as a repository for non-translated mRNAs during acute stress.","PeriodicalId":10918,"journal":{"name":"Current Genetics","volume":"67 6","pages":"857-863"},"PeriodicalIF":2.5,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00294-021-01192-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39004245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3