Current pharmaceutical biotechnology最新文献

{"title":"Molecular Mechanisms of Immune Resistance in Pancreatic Cancer: An Update.","authors":"Wei Ming Pang, Wei Meng Lim, Zi Ni Ngai, Rhun Yian Koh","doi":"10.2174/0113892010365485250330110031","DOIUrl":"https://doi.org/10.2174/0113892010365485250330110031","url":null,"abstract":"<p><p>Pancreatic cancer is an exceptionally aggressive form of cancer with a poor prognosis, primarily due to several factors, one of which is the significant development of immune resistance. Despite new medical perceptions of the interaction between the immune system and tumour, experts have continually explored the molecular mechanisms of immune resistance in pancreatic cancer over the years but have not yet reached a complete understanding. Studying immune resistance is also fundamental because it gives us a better understanding of how to develop highly effective, individualised immunotherapeutic approaches. However, various characteristics can be used to describe the degree of immunological resistance. In the case of pancreatic cancer, the Tumour Microenvironment (TME) is specially structured in a way that it consists of stroma abundantly. Concurrently, it can regulate the secretion and expression of various immunosuppressants, like programmed death-ligand 1 (PD-L1), indoleamine 2,3-dioxygenase (IDO), adenosine, and inosine that impairs the anti-tumour response attributed from the immune system, along with growth factors that contributes to the development of tumour growth. Besides, oncogenic pathways, such as TP53 and KRAS mutation and immunosuppressive cell populations, including T-regulating cells and myeloid-derived suppressor cells collaboratively suppress the immune activity, thereby inducing immune resistance. These complexities present significant challenges in designing effective treatments. Immune checkpoints and mechanisms such as PDL1- mediated MHC-1 downregulation, galectins, autophagy, TP53, and P2RX1-negative neutrophils also contribute to immune resistance. Hence, this review summarises the current knowledge regarding the underlying molecular mechanisms of immune resistance in pancreatic cancer, along with several existing molecular therapeutics and approaches to overcome these barriers.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143982741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combating TB Pathology: Advanced Diagnostics, Innovative Therapies, and Public Health Strategies: A Review.","authors":"Satyajit Mohanty, Manmeet Kaur Khanna, Krishnendu Adhikary, Tuhin Mukherjee, Anwesha Sahu, Ivan Kahwa, Pranav Kumar Prabhakar, Mahendra Pratap Swain, Anasuya Sahoo, Manoj Kumar Parida","doi":"10.2174/0113892010355430250330074301","DOIUrl":"https://doi.org/10.2174/0113892010355430250330074301","url":null,"abstract":"<p><p>Tuberculosis (TB) continues to be a major global health challenge, largely due to the complex nature of Mycobacterium tuberculosis. Its early detection and effective management are heavily reliant on advanced diagnostic methods. New drug delivery systems and repurposing existing drugs show great promise in improving TB treatment. This study explores the progress and hurdles in developing anti-TB drugs, focusing on those currently in clinical trials. Additionally, innovative approaches like immunotherapy, combination therapy, and adjunct therapy, which include the use of phytochemicals, are examined for their potential to enhance treatment outcomes and tackle drug resistance. These innovative approaches could be the key to the future of the fight against TB. It also highlights how these strategies could accelerate TB treatment. It incorporates public health strategies for preventing TB transmission and ensuring patients adhere to treatments. By addressing these key areas, this work aims to contribute to the global fight against TB and improve the lives of those affected by the disease.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"siRNA Therapeutics in Lymphoma: Gene Targets, Delivery Strategies, and Synergy with Chemotherapy.","authors":"Obinna Ikebunwa, Vladislav Turenko, Ikenna Uchendu, Valeriia Kovchina, David Bagaev, Valery Smirnov","doi":"10.2174/0113892010363276250405191808","DOIUrl":"https://doi.org/10.2174/0113892010363276250405191808","url":null,"abstract":"<p><p>Lymphoma is a type of cancer that arises from the lymphatic system, which involves the uncontrollable proliferation of abnormal lymphocytes. Traditionally, lymphoma is classified into Hodgkin and non-Hodgkin lymphoma. To date, chemotherapy remains one of the most common treatments for lymphoma. Notwithstanding the level of efficacy achieved in the use of chemotherapy, the toxicity and drug resistance by cancer cells have been a major obstacle in the treatment of lymphoma. Currently, researchers are exploring the use of siRNA to specifically target and knock down carcinogenic genes involved in lymphoma. The ability of siRNA to target specific genes makes it a good potential substitute for chemotherapeutic agents. Additionally, various delivery systems have also been developed over the years to help mitigate the challenges involved in siRNA delivery to their target genes, which include but are not limited to offtarget effects and systemic breakdown by nucleases. However, there are still many setbacks that need to be resolved to get the desired standard in using only siRNA for lymphoma treatment. Combinations of siRNA with chemotherapy drugs are successful against a variety of molecular targets and can make cancer cells more susceptible to treatment. Therefore, the use of chemotherapy and siRNA might provide the much-needed solution for lymphoma treatment, with reduced toxicity and increased efficacy. Considering the fact that some delivery systems have been developed for the co-delivery of chemotherapeutic agents and siRNA for treating cancers, exploring this option in lymphoma treatment would be a great option towards the right direction and requires better knowledge and development of siRNA therapeutics.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143984755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction Between Staphylococcus aureus and Microbiota: Invasion or Commensalism.","authors":"Ahmad Nasser, Shiva Jahanbakhshi","doi":"10.2174/0113892010364717250404175242","DOIUrl":"https://doi.org/10.2174/0113892010364717250404175242","url":null,"abstract":"<p><p>The term \"Microbiota\" refers to the vast array of symbiotic microorganisms that coexist with their hosts in practically all organs. However, the microbiota must obtain nutrition and minerals from its host to survive; instead, they produce beneficial compounds to protect the host and regulate the immune system. Conversely, pathogenic bacteria utilize their enzymes to independently gain sustenance through an invasive process without almost any beneficial compound production. One of the fully equipped pathogens, Staphylococcus aureus, is present in nearly every organ and possesses a variety of defense and invasion systems including an enzyme, a mineral collection system, a system for detecting environmental conditions, and broad toxins. The microbiota properly can defend its kingdom against S. aureus; however, if necessary, the host immune system is alerted against the pathogen, so this system also acts against the pathogen, a game that can ultimately lead to the death of the pathogen. However, S. aureus can change the host's conditions in its favor by changing the host's conditions and causing inflammation, a condition that cannot be tolerated by the microbiota. In this review, we will explain how microbiota defend against S. aureus.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143984661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Role of Coffee in Thioacetamide-induced Nephrotoxicity: A Study in Rats.","authors":"Syeda Nuzhat Fatima Zaidi, Amna Mohiuddin, Anar Gojayev, Afnan Jan, Abdullatif Bin Muhsinah, Ajmal Khan","doi":"10.2174/0113892010356725250329094402","DOIUrl":"https://doi.org/10.2174/0113892010356725250329094402","url":null,"abstract":"<p><strong>Objective: </strong>The purpose of the present research was to assess the protective role of coffee in thioacetamide-induced nephrotoxicity.</p><p><strong>Methods: </strong>The experimental period consisted of 18 weeks, divided into two phases. Four experimental groups were designed, each consisting of six rats. Group I was considered an untreated control group. Groups II and III were intraperitoneally injected with thioacetamide at a dose of 200 mg/kg body weight twice a week for twelve weeks during the first phase of the study. In the second phase, group II received saline, and group III and group IV received 0.4 mg/Kg of coffee daily for six weeks. The biochemical analysis was evaluated by the estimation of plasma urea, uric acid, creatinine, Malondialdehyde (MDA), Superoxide Dismutase (SOD), and catalase.</p><p><strong>Results: </strong>Thiocetamide-induced nephrotoxicity resulted in the reduction of body weight, superoxide dismutase, and catalase activities, and an increase in kidney weight, plasma urea, uric acid, creatinine, and tissue malondialdehyde. Supplementation with coffee effectively increased body weight while reducing elevated levels of urea, uric acid, creatinine, and MDA. It also restored SOD and catalase activities in Group III (TAA + Coffee-treated).</p><p><strong>Conclusion: </strong>This work shows that coffee can protect the kidneys against thioacetamide-induced nephrotoxicity in a rat model. It highlights the antioxidant potential of coffee by its ability to restore enzymatic antioxidant activity (SOD and catalase), lower oxidative stress markers (MDA), and enhance renal function measures (urea, creatinine, and uric acid). The study fills a significant gap by demonstrating coffee as a viable natural therapeutic agent for oxidative stressinduced kidney impairment, providing an alternative to conventional treatments with fewer side effects.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detailed Comprehensive Review on Advancement of Cubosomes and their Currently Available Applications.","authors":"Raghwendra R Waghmode, Pranay Wal, Seshadri Nalla, Praneeth Ivan Joel Fnu, Virat Kumar Khatri, Pravin Kumar Darji, Binit Patel, Ankita Wal","doi":"10.2174/0113892010347758250122173337","DOIUrl":"https://doi.org/10.2174/0113892010347758250122173337","url":null,"abstract":"<p><p>Cubosomes, nanostructured lipid-based carriers, exhibit a promising potential in drug delivery due to their unique structure, which integrates amphiphilic lipids and polymer-based stabilizers. This review examines the scientific principles of digestion relevant to cubosome function, their structural and compositional aspects, and various processing methods. We also explore potential distribution pathways, drug delivery strategies, and therapeutic applications, including cancer therapy, antimicrobial therapy, vaccine delivery, ocular and dermatological applications, and transdermal delivery. Notably, some challenges still hinder the clinical application of cubosome products, which this review addresses. This paper aims to provide an in-depth discussion on cubosomes, encompassing their composition, theories, processing techniques, and the diverse administration routes that make them a versatile option for targeted drug delivery. A bibliographic review was conducted using major electronic databases (PubMed, ScienceDirect, Scopus, Google Scholar, Springer, and Wiley) along with offline and online academic libraries for comprehensive data collection. Cubosomes, as innovative lipid-based nanotechnology similar to liposomes and niosomes, possess significant potential due to their unique bioadhesive properties, thermodynamic stability, and capability to encapsulate a variety of compounds. Composed typically of glyceryl monooleate and Phytantriol with a stabilizer, cubosomes enable sustained and targeted release, suggesting a broad range of applications. Further advancements are needed to address current limitations in their practical application.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dendrimers-Based Hydrogels and Nanogels for Drug Delivery.","authors":"Deblina D Bhowmik, Juhi S Gupta, Pallavi A Kamandar, Amol D Gholap, Satish Rojekar, Navnath T Hatvate","doi":"10.2174/0113892010347680250209183005","DOIUrl":"https://doi.org/10.2174/0113892010347680250209183005","url":null,"abstract":"<p><p>Current developments in nanotechnology provide an alternative therapy for various diseases by utilizing customized medicine. Among some of the nanoscale superstructures made of the hydrophilic or amphiphilic polymeric matrix are nanogels. At the same time, hydrogels are the first biomaterials created for insertion into the human body and have several biological uses. Owing to the advantages of nanogels and hydrogel, including biocompatibility, hydrophilicity, controlled drug release, and intelligent drug delivery, another macromolecule called dendrimer is incorporated into the nanogel and hydrogel for synergistic effects. In this review, we focus on the applications of dendrimer-based hydrogels and nanogels as carriers for targeted delivery of drugs. We also present the synthetic processes, different characterization methods, and challenges in incorporating the dendrimer and the drug during the preparation of nanogel and hydrogel. In recent years, the most widely used dendrimers for hydrogel formation have been reported to be poly(amidoamine) (PAMAM), phosphorous, peptide, and polyester dendrimers. Dendrimer-based hydrogel and nanogels show various applications in the treatment of a wide range of diseases, such as glaucoma, cancer, and microbial diseases. The self-cleaving mechanism of dendrimer hydrogels (DH) leads to enhanced and sustained delivery of the drugs in the treatment of cancer. Recent studies show the use of doxorubicinconjugated nanogel-based PAMAM dendrimer as a capable nanocarrier for the delivery of drugs in the treatment of cancer.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive Phytochemicals: Unlocking the Promise of Natural Remedies for Diabetes Mellitus.","authors":"Diksha Gulati, Ajmer Singh Grewal, Balraj Saini","doi":"10.2174/0113892010371753250324150813","DOIUrl":"https://doi.org/10.2174/0113892010371753250324150813","url":null,"abstract":"<p><strong>Background: </strong>Metabolic syndrome encompasses conditions such as diabetes mellitus (DM), which has become increasingly prevalent. Chemically synthesized medications are commonly used to mitigate the effects of DM and its complications; however, these often result in undesirable side effects, including weight gain, digestive issues, and heart failure.</p><p><strong>Objective: </strong>This review highlights the therapeutic potential of bioactive compounds and antidiabetic plants that possess proven anti-diabetic properties. Focusing on phytomedicines also explores their possible mechanisms of action and positions this work relative to current reviews in the field.</p><p><strong>Methods: </strong>A comprehensive literature analysis was conducted, emphasizing the therapeutic potential of bioactive compounds in anti-diabetic plants. Databases such as PubMed, Scopus, and Google Scholar were thoroughly searched to identify studies investigating the anti-diabetic properties and mechanisms of action of plant-derived bioactive compounds. Inclusion criteria focused on studies evaluating the pharmacological effects of herbal medicines, plant extracts, and isolated bioactive compounds on diabetes management.</p><p><strong>Results: </strong>Therapeutic plants, as sources of anti-diabetic compounds, offer significant advantages. They are affordable, exhibit minimal or no adverse effects, and do not necessitate strict dietary restrictions or intense exercise regimens. The integrated insights underscore the potential of phytomedicines to address limitations in current diabetes management strategies.</p><p><strong>Conclusion: </strong>The unique focus on phytomedicines positions this review as a valuable resource for researchers and clinicians. Detailing mechanisms and evidence supporting the efficacy of these compounds, guides the development of innovative strategies for identifying and utilizing bioactive compounds in effective diabetes management.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the Mechanism of Tangmaikang Granules in Treating Diabetic Kidney Disease Based On UPLC-MS/MS, Network Pharmacology, Molecular Docking, Molecular Dynamics Simulations, and Experimental Validation.","authors":"Zhixin Wang, Shuqin Liu, Ying Zhang, Huaming Xian, Xinzhu Yuan, Changwei Lin, Xisheng Xie","doi":"10.2174/0113892010369197250321083806","DOIUrl":"https://doi.org/10.2174/0113892010369197250321083806","url":null,"abstract":"<p><strong>Background: </strong>Diabetic Kidney Disease (DKD) is a major cause of End-Stage Renal Disease (ESRD) and lacks effective treatments. Tangmaikang Granules (TMK), a multi-herb traditional Chinese medicine formulation, have shown potential in managing DKD. However, the precise active components, molecular mechanisms, and therapeutic advantages of TMK remain unclear.</p><p><strong>Objective: </strong>This study tests the hypothesis that TMK granules exert protective effects on DKD by targeting multiple pathways involved in oxidative stress, inflammation, and apoptosis in podocytes through a multi-targeted approach. The aim was to identify TMK's bioactive components, evaluate its therapeutic potential, and uncover its molecular mechanisms in DKD.</p><p><strong>Methods: </strong>The bioactive constituents in TMK were determined through ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Drug targets were identified using SwissTargetPrediction and SuperPred, whereas DKD-associated targets were obtained from the GeneCards, DisGeNET, OMIM, and TTD databases. A Protein-Protein Interaction (PPI) network was constructed, and key targets were identified via topological analysis. Molecular docking and dynamics simulations were performed to evaluate stable binding interactions. GO and KEGG pathway enrichment analyses were conducted to uncover relevant signaling pathways. TMK's effects on oxidative stress, inflammation, and apoptosis in podocytes were assessed using CCK-8, flow cytometry, RT-qPCR, ELISA, and Western blot assays.</p><p><strong>Results: </strong>Thirty active compounds and 384 potential therapeutic targets were identified, with eight key targets. Pathway enrichment analysis revealed TMK's involvement in AGE-RAGE, EGFR, HIF-1, and apoptosis pathways, affecting inflammatory cytokine responses and oxidative stress. In vitro experiments demonstrated that TMK significantly reduced oxidative stress, inflammation, and apoptosis in podocytes by inhibiting the MAPK and NF-κB pathways.</p><p><strong>Conclusion: </strong>TMK granules target DKD through a multi-component, multi-target strategy, effectively mitigating oxidative stress and suppressing inflammatory and apoptotic pathways. This study integrates advanced computational and experimental methods, demonstrating TMK's unique therapeutic potential and providing a robust foundation for its clinical application in DKD management.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Effects of Lactobacillus acidophilus and Balanites aegyptiaca in Managing Hyperglycemia, Oxidative Stress, and Inflammation in Diabetic Rats.","authors":"Dalia Elebeedy, Nada Elgendy, Aml Ghanem, Aya Salem, Muhammad Alaa Eldeen, Mohamed A Ali, Dalal Sulaiman Alshaya, Maha A Aljumaa, Hanadi A Katouah, Eman Fayad, Ghadi Alsharif, Ahmed I Abd El Maksoud","doi":"10.2174/0113892010362400250121075011","DOIUrl":"https://doi.org/10.2174/0113892010362400250121075011","url":null,"abstract":"<p><strong>Background: </strong>Diabetes mellitus is a chronic condition characterized by persistent hyperglycemia, which can lead to serious complications if not managed effectively. Recent interest has grown in exploring the potential of probiotics and prebiotics as complementary therapies. This study investigates the combined effects of the probiotic Lactobacillus acidophilus and the prebiotic Balanites aegyptiaca on blood glucose levels, oxidative stress, and inflammatory markers in an alloxan-induced diabetic rat model.</p><p><strong>Methods: </strong>A total of 40 male rats (100-110 g; 8-10 weeks) were randomly divided into five groups: a normal control group, a diabetic control group, and diabetic groups treated with Lactobacillus acidophilus, Balanites aegyptiaca, or their combination. The mice in the diabetic groups were orally treated with 28.5 mg/kg of Lactobacillus acidophilus, Balanites aegyptiaca, or both twice daily for 30 days. Blood glucose levels were measured before and after treatment. Antioxidant enzyme activities, including glutathione peroxidase, glutathione reductase, glutathione Stransferase, catalase, and superoxide dismutase, were assessed. Inflammatory cytokines (TNF-α and IL-6) and lipid profiles (cholesterol and triglycerides) were also evaluated.</p><p><strong>Results: </strong>The combination of Lactobacillus acidophilus and Balanites aegyptiaca significantly reduced blood glucose levels in diabetic rats from 295 mg/dl to 114 mg/dl, outperforming the effects of either treatment alone. Antioxidant enzyme activities were markedly enhanced in the combined treatment group, indicating reduced oxidative stress. The combined therapy also significantly decreased pro-inflammatory cytokines TNF-α and IL-6 compared to the diabetic control group. Furthermore, lipid profiles showed improved cholesterol and triglyceride levels in the combined treatment group, even in a high-lipid diet.</p><p><strong>Conclusion: </strong>The combination of Lactobacillus acidophilus and Balanites aegyptiaca reduced hyperglycemia, oxidative stress, and inflammation in diabetic rats, suggesting potential as a complementary diabetes therapy.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}