Current pharmaceutical biotechnology最新文献

{"title":"Spray Drying: A Promising Technique for Inhalable Vaccine Development.","authors":"Sridhar Vemulapalli, Satish Rojekar, Manit Gandhi, Bhavesh Patel, Amitkumar Virani, Purva Patel, Kinjal Parikh","doi":"10.2174/0113892010352443250402184623","DOIUrl":"https://doi.org/10.2174/0113892010352443250402184623","url":null,"abstract":"<p><p>In the pursuit of innovative vaccine delivery methods, this review explores the potential of spray drying for formulating inhalable vaccines. Traditional vaccine approaches face challenges in administration, storage, and accessibility, especially in resource-limited settings. Inhalable vaccines, utilizing techniques like spray drying, offer a promising solution. By bypassing systemic circulation and directly targeting the respiratory mucosa, inhalable vaccines can induce robust mucosal and systemic immune responses. Spray drying, a versatile technique, is particularly well-suited for formulating inhalable vaccines. It transforms liquid vaccine formulations into finely dispersed powders, enabling efficient delivery to the lungs. This review delves into the unique characteristics of spray-dried particles, their impact on immune system activation, and their role in overcoming traditional vaccine limitations. The exploration emphasizes the potential for spray drying to revolutionize vaccine development, providing a comprehensive overview of its applications and contributions to improving global public health.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144752647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging Protein Therapeutics as a Strategy for Cervical Cancer Treatment.","authors":"Srishti Sharma, Anuja Mishra, Pratibha Pandey, Meenakshi Verma, Ashok Kumar Bishoyi, Renuka Jyothi S, Sorabh Lakhanpal, Laxmidhar Maharana, Ashish Singh Chauhan, Mohammad Mustufa Khan, Fahad Khan","doi":"10.2174/0113892010397753250704105423","DOIUrl":"https://doi.org/10.2174/0113892010397753250704105423","url":null,"abstract":"<p><p>Cervical cancer continues to be a critical public health concern globally, with increasing mortality rates, particularly in Low- and Middle-Income Countries (LMICs) where healthcare resources remain limited. With more than 300,000 fatalities each year, it is the fourth most frequent cancer in women globally. Long-term infection with carcinogenic Human Papillomavirus (HPV) variants, which cause cancer through viral proteins including E5, E6, and E7, is the leading cause of cervical cancer. These proteins interfere with host cellular functions, which promote the development and spread of cancer. Conventional treatment strategies, including chemotherapeutics and immunotherapies, have achieved varying degrees of success. However, protein-based therapeutics have recently emerged as a promising class of agents in oncology due to their ability to modulate specific molecular targets with high precision and specificity. These biologics interact with cell surface receptors and orchestrate essential signalling cascades, such as the NF-κB, MAPK, and PI3K/AKT pathways. Notably, new classes of protein therapeutics, such as toxin-based agents and Bromodomain and Extra-Terminal (BET) domain inhibitors, have shown effectiveness in disrupting tumor-promoting pathways. In addition to their direct antitumor activities, protein therapeutics also modify the tumor microenvironment, affecting stromal elements and lymphatic architecture, and ultimately promoting apoptosis. This review critically examines the landscape of protein-based therapeutic approaches for cervical cancer, delineating their mechanisms of action and highlighting their role in targeting inflammatory pathways-such as inflammasomes and cytokine networks-that contribute to tumor progression and immune modulation.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological and Pharmaceutical Application of β-galactosidase Stabilized on Surface-modified Silica Nanoparticles.","authors":"Shakeel Ahmed Ansari, Ahmed A Damanhory, Doha Zakaria Sija, Rukhsana Satar","doi":"10.2174/0113892010395368250630161542","DOIUrl":"https://doi.org/10.2174/0113892010395368250630161542","url":null,"abstract":"<p><strong>Introduction: </strong>Nanoparticles used in enzyme immobilization offer a high surface area- to-volume ratio, high chemical and thermal stability, and resistance to microbial attack.</p><p><strong>Methods: </strong>The present investigation demonstrates the immobilization of Aspergillus oryzae β- galactosidase on silica nanoparticles via covalent binding. A greater yield of enzyme immobilization (89%) was attained on the developed nanobiocatalyst.</p><p><strong>Results: </strong>It was observed that the immobilized and soluble enzymes had optimal pH and temperature values of 50 °C and 4.5, respectively. It was monitored that at pH 4.0, soluble β- galactosidase (SβG) exhibited 59% activity. However, the immobilized enzyme showed 92% activity under identical conditions. Similarly, 41% enzyme activity was retained at 70 oC by the free enzyme. Conversely, immobilized β-galactosidase (IβG) retained 70% activity under similar experimental conditions. Additionally, it was observed that at 5% galactose concentration, IβG showed 55% activity under one hour of incubation. However, under comparable experimental conditions, SβG showed 24% activity.</p><p><strong>Discussion: </strong>It was observed that the immobilized enzyme was reusable, maintaining 90% of its activity even after five uses. The soluble enzyme demonstrated 62% and 70% lactose hydrolysis under the same conditions after 8 hours, while IβG demonstrated 74% and 85% lactose hydrolysis at 40°C and 50°C, respectively, in a controlled batch reactor experiment that was run for 10 hours.</p><p><strong>Conclusion: </strong>Hence, owing to the greater reusability (90% after 5th repeated use) and excellent conversion of lactose at higher temperatures, the developed nanosupport may be used to produce lactose-free dairy products in continuous reactors on a large scale in biotechnology industries.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in the Mechanism and Applications of Stimulus-Responsive DNA Hydrogels.","authors":"Yifan He, Zhaohe Huang, Xiaojing Pei, Yinmao Dong, Xiangliang Yang","doi":"10.2174/0113892010374050250707181128","DOIUrl":"https://doi.org/10.2174/0113892010374050250707181128","url":null,"abstract":"<p><p>DNA hydrogels possess numerous unique and attractive features, including excellent biocompatibility and biodegradability, as well as inherent programmability, catalytic functionality, therapeutic potential, and precise molecular recognition and bonding capabilities. Furthermore, intelligent DNA hydrogels exhibit stimuli-responsive behaviors, transitioning between gel and sol states in response to various stimuli, including pH, temperature, enzymes, and others. Through intelligent, rational design and controlled preparation of DNA nanostructures, a broad spectrum of advanced applications has been realized. In this minireview, we focus on recent developments in the construction strategies, molecular structures, and functional mechanisms of DNA hydrogels. Additionally, representative applications of stimuli-responsive DNA hydrogels are discussed. Finally, challenges and the future outlook of DNA hydrogels are proposed.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Immunogenetic Mechanisms in Parkinson's Disease Using Single-Cell Transcriptomics and Mendelian Randomization.","authors":"Dongyuan Xu, Yu Lei, Ji Wu, Keyu Chen, Songshan Chai, Nanxiang Xiong","doi":"10.2174/0113892010378080250711022253","DOIUrl":"https://doi.org/10.2174/0113892010378080250711022253","url":null,"abstract":"<p><strong>Introduction: </strong>Parkinson's disease (PD) is a prevalent neurodegenerative disorder characterized by the progressive loss of dopaminergic neuron. Although the role of immunity in PD has been increasingly recognized, the immunogenetic mechanisms underpinning its progression remain largely unresolved.</p><p><strong>Methods: </strong>We employed an integrative approach combining Mendelian randomization (MR), expression quantitative trait loci analysis, and single-cell RNA sequencing to investigate immune cell infiltration and transcriptional regulation in PD. Immune cell composition, pathway activation, and gene regulatory networks were assessed through single-cell gene set enrichment analysis and transcriptional correlation analyses.</p><p><strong>Results: </strong>Immune profiling revealed significant increases in naive B cells (1.22-fold), plasma cells (3.00-fold), switched memory B cells (2.85-fold), and unswitched memory B cells (6.70- fold) in PD patients compared to controls (p < 0.001). MR analysis identified five causal genes- CYTH4, FGR, LRRK2, RIN3, and SAT1- associated with monocyte, neutrophil, and B cell infiltration. SAT1 (OR: 1.529; 95% CI: 1.018-2.297) and RIN3 (OR: 1.222; 95% CI: 1.039- 1.437) showed strong associations with PD risk (p < 0.01). SAT1 positively correlated with PARK7 and regulated reactive oxygen species signaling, while FGR negatively correlated with ABCA4, influencing lipid metabolism and immune responses.</p><p><strong>Discussion: </strong>These findings highlight distinct immunogenetic mechanisms driving PD progression. The SAT1-PARK7 axis appears to modulate oxidative stress and neuroinflammation, whereas the FGR-ABCA4 interaction may affect metabolic and immune pathways. While the study is limited by population heterogeneity and the challenges of inferring causality, it provides mechanistic insights into immune contributions to PD.</p><p><strong>Conclusion: </strong>Our integrative genomic analysis identified novel regulatory networks involving immune-related genes in PD, offering potential targets for mechanistic understanding and therapeutic development.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144689416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethanolic Extract of Cyperus rotundus Augments Chemosensitivity to Docetaxel and Suppresses Autophagic Flux in HER2-Positive Breast Cancer Cells.","authors":"Xiaoli Bian, Chao Li, Xiaoyu Liu, Zhaoyun Liu, Xiang Song, Fukai Wang, Xinzhao Wang, Wenna Shao, Haiyin Sun, Zhiyong Yu","doi":"10.2174/0113892010374529250715164342","DOIUrl":"https://doi.org/10.2174/0113892010374529250715164342","url":null,"abstract":"<p><strong>Introduction: </strong>Breast cancer (BC) represents a malignancy affecting populations globally. Its incidence is on the rise. The ethanolic extract of Cyperus rotundus (EECR) has demonstrated potent anticancer activities against multiple human cancer types, inducing apoptosis in BC cells. Autophagic flux protects HER2+ cancer cells from trastuzumab-induced cytotoxicity, so inhibiting it undermines the resistance phenotype. This study aimed to elucidate the therapeutic potential of EECR in trastuzumab-resistant HER2-positive BC and decipher its underlying mechanisms.</p><p><strong>Methods: </strong>Colony formation assay and Cell Counting Kit-8 (CCK-8) assessed cell viability. Flow cytometry was used for cell cycle analysis and apoptosis detection. Western blotting quantified relevant protein expressions. Nude mice were euthanized prior to tissue harvest. Tumor tissues were excised and processed for histological examination, with 5 μm paraffin sections prepared on glass slides for hematoxylin and eosin (H&E) staining. An orthotopic JIMT-1 cell transplantation tumor model was established, and immunohistochemistry was conducted.</p><p><strong>Results: </strong>EECR demonstrated a dose-dependent suppressive effect on HER2-positive BC cells, inducing apoptosis and G2-M phase cell cycle arrest. It inhibited autophagic flux, as evidenced by LC3 and p62/SQSTM1 accumulation, and upregulated raptor and phosphorylated Mitogen- Activated Protein Kinase (MAPK) in trastuzumab-resistant JIMT-1 cells. Phosphorylated ERK (pERK)/total ERK and Raptor levels were significantly elevated in EECR-treated JIMT-1 cells compared to other treatment groups. Furthermore, EECR significantly inhibited tumorigenic growth in JIMT-1 cells.</p><p><strong>Conclusion: </strong>This study reveals that EECR effectively impedes autophagic flux in trastuzumabresistant HER2-positive breast cancer cells, a mechanism increasingly recognized as central to therapeutic resistance. By promoting LC3B and p62 accumulation and modulating the MAPK/mTOR signaling axis, EECR not only disrupts a key survival pathway in resistant cells but also enhances the efficacy of standard chemotherapeutic agents like docetaxel. These dual effects-autophagy inhibition and chemosensitization-underscore EECR's therapeutic potential as an adjuvant strategy to overcome trastuzumab resistance. Given its multi-target nature and favorable safety profile, EECR represents a promising candidate for future combination therapy in refractory HER2-positive breast cancer.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144689415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation and Assessment of Broccoli Extract-Infused Hydrogel for Targeted Breast Cancer Therapy.","authors":"Kajal -, Mohammad Rashid Khan, Minhaj Ahmad Khan, Pratibha Pandey, Fahad Khan","doi":"10.2174/0113892010373087250710035336","DOIUrl":"https://doi.org/10.2174/0113892010373087250710035336","url":null,"abstract":"<p><strong>Introduction: </strong>The most prevalent kind of cancer among women is breast cancer. Consequently, the development of novel, potent medications with fewer adverse effects is required to treat it. Breast cancer is frequently treated clinically with chemotherapy and surgery. However, there are still significant challenges to be addressed in the treatment of breast cancer, including inadequate therapeutic results, inevitable side effects, and the surgical excision of breast tissue. The objective of the study is to develop broccoli extract-based Hydrogel to overcome the challenges in breast cancer treatment.</p><p><strong>Methods: </strong>The developed Hydrogel was characterized by certain techniques to check its stability and drug release abilities. Swelling studies and drug release behavior were checked; the porosity of Hydrogel was checked by SEM EDX Analysis. Furthermore, in vitro studies were done to check the anti-breast cancer activity of the developed Hydrogel.</p><p><strong>Results: </strong>The hydrogel was a highly porous structure with and compressive modulus, which makes it good for biological use in drug delivery. The in vitro studies showed that, developed Hydrogel inhibits the growth of breast cancer cells (MCF-7) at different concentrations and time intervals of 24 and 48 Hrs and was compatible with the non-cancerous cell line 3T3-L1. The results indicate the tolerability of Hydrogel at the level of cells.</p><p><strong>Discussions: </strong>Numerous investigations have demonstrated the anticancer effects of SFN by influencing the various biological processes that tumor cells engage in. In breast cancer cell lines, SFN functions as an HDAC inhibitor and reduces the expression of ER, EGFR, & HER-2 proteins. SFN also triggers apoptosis and cell cycle halt. Both Hydrogel and SFN inhibit the cells growth in MCF-7 breast cancer cells and agree with the previous studies.</p><p><strong>Conclusion: </strong>In conclusion, we synthesized a hydrogel using broccoli extract to treat breast cancer with better stability, tolerance, and effectiveness through sustained local drug delivery. It was determined that this new hydrogel was a simple and affordable way to accomplish the continuous gene release feature, which would enhance the therapeutic efficacy in anti-cancer treatment while reducing the likelihood of potentially fatal side effects.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144689417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Polymer-Based Nanoparticles for Biomedical and Industrial Applications.","authors":"Fahad Y Sabei","doi":"10.2174/0113892010386798250708153155","DOIUrl":"https://doi.org/10.2174/0113892010386798250708153155","url":null,"abstract":"<p><p>Polymeric nanoparticles (PNPs) are considered to be a revolutionary method for drug delivery and offer significantly more advantages than conventional drug delivery systems. This review synthesizes recent research on biodegradable polymers in drug delivery, emphasizing their properties, modifications, toxicity, and applications in drug absorption. It consolidates key insights from 193 research papers to offer a comprehensive overview of the field, addressing existing research gaps and highlighting various applications. Polymers can be classified based on their structure, source, and biodegradability, which are crucial for assessing their environmental impact and suitability for various applications. Polymers are categorized into two main groups based on biodegradability: biodegradable and non-biodegradable. The primary aim of this review is to elucidate the diverse applications of natural and synthetic biodegradable polymeric nanoparticles, which include cancer treatment, diabetes management, pulmonary drug delivery, and the treatment of ocular infections, all of which are thoroughly explored in this review. Additionally, the role of polymer-based hydrogels is explored as a promising solution in drug delivery. These hydrogels address issues such as poor stability and enhance treatment efficacy by ensuring the sustained release of drugs.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of the β-Myrcene Rich Essential Oil from Astronium Urundeuva (M.Allemão) Engl. (Anacardiaceae) to Potentiate Fluconazole Activity and Inhibit Morphological Transition in Candida Species.","authors":"Jose Thyalisson da Costa Silva, Saulo Almeida Meenezes, Maria Hellena Garcia Novais, Naiza Saraiva Farias, Adrielle Rodrigues Costa, Francisco Sydney Henrique Felix, Ademar Maia Filho, Murilo Felipe Felicio, Nadilânia Oliveira da Silvaa, Ginna Gonçalves Pereira, Cicero Dos Santos Leandro, Alison Honorio de Oliveira, Lariza Leisla Leandro Nascimento, Luiz Filipi Teles Feitosa, Julimery Gonçalves Ferreira Macedo, Maria Flaviana Bezerra Morais-Braga, Henrique Douglas Melo Coutinho, Natália Cruz-Martins, José Weverton Almeida-Bezerra","doi":"10.2174/0113892010359736250707113515","DOIUrl":"https://doi.org/10.2174/0113892010359736250707113515","url":null,"abstract":"<p><strong>Background: </strong>In view of the increasing resistance of Candida species, it is necessary to explore alternative strategies. In this context, essential oils have emerged as promising options, among which the essential oil of Astronium urundeuva (M. Allemão) Engl. has shown potential, as it is traditionally used in folk medicine for the treatment of inflammation and multiple infections. Thus, the aim of this study was to evaluate the chemical profile, anti- Candida activity, and Fluconazole (FCZ) potentiating effect of the essential oil extracted from the leaves of A. urundeuva (EOAU) and its ability to inhibit the virulence mechanism in Candida species.</p><p><strong>Methods: </strong>The essential oil was obtained via hydrodistillation and characterized using gas chromatography-mass spectrometry. To evaluate the antifungal effects and the modulating activity of Fluconazole (FCZ), the essential oil was diluted in DMSO (1 mL) and SDB medium (9 mL) and tested on 3 Candida strains using the serial microdilution method. In addition, a morphological transition assay was used to evaluate its capacity to inhibit fungal virulence.</p><p><strong>Results: </strong>The major constituent of EOAU was the monoterpene β-myrcene (71.07%). The results indicate that the essential oil exhibits an antifungal effect, with C. tropicalis being the most susceptible species. At subinhibitory concentrations (MC/8), the EOAU enhanced the action of fluconazole against C. krusei and C. tropicalis. The EOAU strongly inhibited the morphological transition in C. tropicalis.</p><p><strong>Conclusion: </strong>EOAU is rich in β-myrcene and exhibits an interesting fungistatic effect, making it a great natural candidate for inhibiting Candida spp. virulence.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144636475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the Influence of Culture Conditions on Mesenchymal Stem Cells: A Transcriptome Sequencing Study.","authors":"Bin Wang, Jiang Xie, Bo Pang, Fang Dong, Junna Zhou, Huanzhang Zhu","doi":"10.2174/0113892010375906250619115932","DOIUrl":"https://doi.org/10.2174/0113892010375906250619115932","url":null,"abstract":"<p><p><p>Aims: To optimize the culture process of Mesenchymal Stem Cells (MSCs) and enhance their biological functions. </p><p> Background: MSCs have shown great potential in treating various diseases due to their low immunogenicity and potent paracrine effects. However, the inherent heterogeneity of MSC populations, which can vary depending on the culture conditions, may challenge large-scale clinical application.</p><p> Objective: This study investigates the inconsistency of MSCs cultured in different media, from the transcriptional level to biological functions.</p><p> Method: RNA sequencing was used to identify different expressed genes of MSCs separated and expanded in three media, which were then validated with qPCR. In vitro assays, including proliferation, tube formation, wound healing, multilineage differentiation, paracrine secretome and injured hepatocyte protection assay, were performed to verify the potential differences among three groups.</p><p> Result: MSCs cultured in platelet lysate-containing medium exhibited high expression of genes involved in extracellular matrix regulation, collagen metabolic processes, and angiogenesis, whereas those cultured in serum-free medium demonstrated high expression of genes associated with DNA replication and chromosome segregation. MSCs cultured under serum-containing medium indicated high levels of genes associated with extracellular matrix regulation, cartilage development, and chemotaxis. The results of functional comparative experiments were consistent with the differences in their gene expression patterns. Notably, MSCs cultured in the serum- containing system exhibited greater protective effect against hepatocyte activity.</p><p> Conclusion: Different culture conditions affect the biological functions of MSCs. Optimal conditions should be investigated for applications. Next, an in vivo model should be established to evaluate differences in MSC tissue repair function under various culture conditions.</p>.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144625559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}