Current pharmaceutical biotechnology最新文献

{"title":"Progress and Application of Multifunctional Ultrasound Theranostic Agents.","authors":"Kejing Fang, Jincan Lei, Yi Zhao, Yujie Feng, Haiyun Huang, Changjun Hou","doi":"10.2174/0113892010352224250304080131","DOIUrl":"https://doi.org/10.2174/0113892010352224250304080131","url":null,"abstract":"<p><p>Ultrasound is an indispensable technology in the biomedical field. With the continuous integration and development of ultrasound medical technology, its potential application value in disease diagnosis and treatment has become increasingly prominent. As the technical core, novel multifunctional ultrasound theranostic agents have been the main focus of research. Here, we summarized various types of multifunctional ultrasound agents, presented their latest applications in important areas, and discussed subsequent research priorities. We hope that with the combination of new technologies, multifunctional ultrasound agents can play a greater role in the diagnosis and treatment of diseases, further promoting the extensive and in-depth development of ultrasound medical technology.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-vitro, In-vivo and Molecular Docking Studies on Dietary Supplement Containing Polar and Non-Polar fractions of Persea Americana for Management of Diet-related Chronic Diseases.","authors":"Doha A Mohamed, Gamil Ibrahim, Hoda B Mabrok, Ibrahim Hamed","doi":"10.2174/0113892010357755250227060845","DOIUrl":"https://doi.org/10.2174/0113892010357755250227060845","url":null,"abstract":"<p><strong>Background: </strong>Diet-related chronic diseases, such as cardiovascular diseases, obesity, diabetes, autoimmune diseases and cancer, are largely preventable with a healthy diet and lifestyle. Therefore, searching for dietary supplements rich in antioxidant and anti-inflammatory phytochemicals for the prevention and/or management of diet-related chronic diseases is an important strategy for controlling these diseases to reduce healthcare costs and sustain development.</p><p><strong>Objective: </strong>The aim of the current research was to prepare dietary supplements from avocado fruit pulp [AFPDS] and evaluate their potential against various diet-related chronic diseases through in-vitro, in-vivo, and molecular docking studies.</p><p><strong>Methods: </strong>Volatile compounds of avocado pulp were evaluated, and the total phenolic compounds, fatty acids, and phytosterols profiles of the AFPDS were determined.</p><p><strong>Results: </strong>D-limonene, methyl propanoate, isobutyl propanoate and pentanol were the principal volatile compounds in the avocado pulp. Total phenolic and flavonoids were present in the AFPDS by 9.65 mg GAE/g and 6.87 mg CE/g, respectively. Chlorogenic acid and cinnamic acid were the major and minor identified phenolic compounds in AFPDS, respectively. Oleic acid [75.06%] and β-Sitosterol [2.19%] were the highest fatty acid and phytosterol present in AFPDS, respectively. AFPDS recorded anti-inflammatory activity against nitric oxide [NO] production in RAW264.7 macrophages by 98.2μg/ml [IC50] and 164.8μg/ml [IC90]. AFPDS showed significant anti-inflammatory activity against carrageenan-induced rat paw edema. AFPDS showed antioxidant activity against DPPH and ABTS by 8.67 mg TE/g and 6.14 mg TE/g. AFPDS possessed anti-cancer activity against MCF7 and HPG2 at10.8μg/ml and 40.5μg/ml, respectively. AFPDS exhibited anti-diabetic activity as an inhibitor of α-amylase and α-glucosidase by26.35±0.77μg/ml and 0.55±0.163mg/ml, respectively. Molecular docking studies revealed high binding affinity of different active compounds present in AFPDS with cyclooxygenase- 2, glutathione peroxidase, α-glucosidase and B-cell lymphoma-extra-large proteins.</p><p><strong>Conclusion: </strong>AFPDS can be considered a new agent for the prevention and treatment of dietrelated chronic diseases, such as diabetes and cancer, due to its anti-inflammatory, antioxidant, anticancer, and anti-diabetic activities, as demonstrated through in-vivo, in-vitro, and molecular docking studies.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochanin-A: A Potential Candidate for the Treatment of Alzheimer's Disease.","authors":"Niraj Kumar Singh, Bharat Bhushan","doi":"10.2174/0113892010362795250223160707","DOIUrl":"https://doi.org/10.2174/0113892010362795250223160707","url":null,"abstract":"<p><p>Alzheimer's disease (AD), the most common form of dementia, is a multifactorial neurological condition characterized by progressive loss of memory and learning, uncontrollable movement, difficulty processing visual images, and impairment of reasoning and/or judgment skills. Although the exact cause of AD is still unknown, recent evidence suggests that environmental, lifestyle, and genetic factors are common contributors to the disease's progression. Pathophysiological features of AD include amyloid beta (Aβ) accumulation, abnormal deposition of neuritic plaques and neurofibrile tangles, cholinergic dysfunction, neuroinflammation, and oxidative stress burden along with mitochondrial dysfunction. There are currently no pharmaceutical methods or medications that can stop the progression of a disease. More attention is now being paid to natural products, herbal medicines, and different bioactive phytoconstituents, particularly flavonoids, as alternative therapies and useful resources for finding new drug candidates for the treatment of AD-like symptoms. A dietary isoflavone, biochanin-A, which is isolated from the leaves and stems of Trifolium pretense L. (family: Leguminosae), possesses remarkable anti-inflammatory and antioxidant properties along with cognitive-enhancing effects. Biochanin-A exhibits notable neuroprotective effects by reducing Aβ deposition, decreasing apoptosis, and preventing the production of pro-inflammatory mediators, including TNF-α, IL- 1β, and NO. Various preclinical reports explore the pharmacological role of biochanin-A against experimentally-induced AD and highlight that it can alter numerous signaling pathways, including Nrf2, NF-κB, JNK, MAPK, and Bcl-2/Bax. The present review article summarizes the numerous research studies that have evaluated the role of biochanin-A for dementia associated with AD. As part of a comprehensive program, biochanin-A has very exceptional potential to prevent and treat AD-related cognitive impairment. It is envisaged that these potential chemical moieties can be employed in the drug discovery process to identify efficacious and safe therapy for the treatments for AD-like manifestation.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of Polydeoxyribonucleotide Nanoliposomes and their Applicability to Cosmetic Formulations.","authors":"Yifan He, Li Xu, Xiaojing Pei, Yinmao Dong, Xiangliang Yang","doi":"10.2174/0113892010353418250218064202","DOIUrl":"https://doi.org/10.2174/0113892010353418250218064202","url":null,"abstract":"<p><strong>Introduction: </strong>The use of polydeoxyribonucleotide (PDRN) in promoting tissue repair and anti-aging has been hindered by several challenges, including its large molecular weight, susceptibility to decomposition, low bioavailability, and poor stability and skin permeability. Liposome formulation technology has emerged as a promising method in cosmetics, enhancing the penetration and protection of active ingredients.</p><p><strong>Method: </strong>In this study, a PDRN-loaded nanoliposomal (PDRN-NL) formulation, which exhibited an average particle size of 125 ± 1 nm, a polydispersity index (PDI) of 0.12 ± 0.02, a zeta potential of -52.6 ± 0.8 mV, and an encapsulation efficiency of 81.3%. The stability of the PDRN-NL and its formulations was assessed using the Turbiscan Lab stability analyzer, which predicted a shelf life of up to three years. Furthermore, the in vitro permeability of the PDRN-NL was evaluated using the Franz diffusion cell method.</p><p><strong>Results: </strong>Results indicated that the cumulative skin permeation of PDRN-NL over 24 hours was 1.22 times higher than that of free PDRN, with skin retention of PDRN-NL being 1.40 times greater. The in vitro release studies demonstrated that liposomal encapsulation not only enhanced permeability but also provided a sustained-release effect and improved stability of PDRN.</p><p><strong>Conclusion: </strong>Overall, the properties of liposome-encapsulated PDRN were significantly enhanced, presenting a novel solution for the incorporation of PDRN in the development of skincare products.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the Anti-Fatigue Effects of Polysaccharides from Erjing Formula.","authors":"Zicheng Lin, Mingshuo Yang, Jinsui Liu, Can Yang, Lixuan Liang, Jiaqi Pan, Quan Yang, Liming Pan","doi":"10.2174/0113892010360130250216043643","DOIUrl":"https://doi.org/10.2174/0113892010360130250216043643","url":null,"abstract":"<p><strong>Background: </strong>The traditional Chinese recipe \"Erjing Formula\", composed of HuangJing and GouQi, is recognized for its tonifying and revitalizing properties, but the active components responsible for these effects are poorly explored. This study investigated the antifatigue effects of Erjing Formula polysaccharides (EJFP).</p><p><strong>Methods: </strong>Mice were treated with EJFP and subjected to weight-loaded swimming tests. Biochemicals were measured, while muscle changes were analyzed by H&E staining and protein expression by Western blotting.</p><p><strong>Objective: </strong>The present study aimed to investigate the anti-fatigue effects of EJFP through weight-loaded swimming tests in mice.</p><p><strong>Methods: </strong>SPF-grade healthy male rat species KM mice were gavaged with EJFP and then subjected to a weight-loaded swimming test. Later, the levels of various biochemical indicators, including blood lactate (BLA), blood urea nitrogen (BUN), superoxide dismutase (SOD), malondialdehyde (MDA), muscle glycogen (MG), and hepatic glycogen (HG), were measured. The muscle tissue slices were analyzed using hematoxylin and eosin H&E) staining. Additionally, the expression levels of Kelch-like ECH-associated Protein 1 (Keap-1) and nuclear factor erythroid 2-related factor 2 (Nrf2) in the muscle tissue of each group were detected using Western Blotting.</p><p><strong>Results: </strong>EJFP (Erjing Formula Polysaccharides) significantly increased swimming time (p ≤ 0.01), reduced oxidative stress markers and detrimental metabolites (BLA, BUN, and MDA), and elevated SOD, MG, and HG levels. Histologically, it improved muscle integrity. Nrf2 expression increased, indicating a relationship between antioxidant effects and antifatigue activity.</p><p><strong>Conclusion: </strong>EJFP demonstrated anti-fatigue effects, mainly at moderate doses, by modulating the Nrf2/Keap1 pathway and improving glycogen and antioxidant levels. This is the first study to document the anti-fatigue effects of Erjing Formula polysaccharides, establishing a theoretical basis for practical applications in the development of anti-fatigue products.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143467262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estradiol Promotes Endometriosis Progression Via the ERβ/QKI /circSMAD2 Axis.","authors":"Yuan Peng, Wenqian Xiong, Haitang He, Hengwei Liu, Tian Fu, Xuefeng Long, Xiaoou Li, Xin Dai, Ying Xu, Ling Zhang, Yi Liu","doi":"10.2174/0113892010331129250216041033","DOIUrl":"https://doi.org/10.2174/0113892010331129250216041033","url":null,"abstract":"<p><strong>Aims: </strong>The present study aimed to examine the roles of circRNA-circSMAD2 and its regulatory mechanisms in endometriosis (EMs).</p><p><strong>Background: </strong>Evidence has confirmed that circRNAs play multiple roles in regulating the occurrence and development of EMs, but the regulatory mechanisms of circRNAs in EMs remain largely unknown.</p><p><strong>Objective: </strong>The roles and regulatory mechanisms of circSMAD2 in EMs.</p><p><strong>Method: </strong>Eutopic and ectopic endometrium of ovarian EMs as well as normal endometrial tissues, were used to extract circRNA, mRNA, and total proteins. The human endometrial stromal cell lines (ThESCs) and endometrial stromal cells (ESCs) were stimulated with different concentrations or times of 17β-estradiol (E2). The mouse model of EMs was established by implanting uterine horns onto the peritoneum wall using a suture.</p><p><strong>Result: </strong>Compared with normal tissues, the expression of circSMAD2 was significantly decreased in eutopic and ectopic endometrial tissues. Furthermore, the expression of circSMAD2 was downregulated by E2 in a dose- and time-dependent manner in ThESCs and ESCs. Overexpression of circSMAD2 inhibited the invasion and migration of ThESCs, while knockdown of circSMAD2 exerted the opposite effect. The RNA binding protein quaking (QKI), which is involved in circRNA formation, was lower in eutopic and ectopic endometrial tissues compared to normal tissues.</p><p><strong>Conclusion: </strong>Moreover, E2 suppressed the expression of circSMAD2 by inhibiting the expression of QKI. Additionally, E2 enabled the expression of estrogen receptor beta (ERβ) to inhibit the expression of QKI and circSMAD2 in vitro and in vivo.</p><p><strong>Conclusion: </strong>The E2/ERβ/QKI/circSMAD2 pathway was involved in cellular migration and invasion in EMs.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143467260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Molecular Docking Techniques for Targeting Protein Misfolding in Neurodegenerative Diseases.","authors":"Kuldeep Singh, Jeetendra Kumar Gupta, Shiv Narayan, Ketki Rani, Divya Jain, Prateek Porwal, Mukesh Chandra Sharma, Shivendra Kumar","doi":"10.2174/0113892010298545241108062449","DOIUrl":"https://doi.org/10.2174/0113892010298545241108062449","url":null,"abstract":"<p><p>Neurodegenerative diseases, such as Alzheimer's, Parkinson's, and Huntington's disease, represent a significant global health challenge with limited therapeutic options. Protein misfolding and aggregation, a common pathological hallmark in these disorders, have emerged as promising targets for therapeutic intervention. Molecular docking techniques have played a pivotal role in the identification and design of small molecules that can modulate protein misfolding, offering new hope for effective treatments. This review provides an overview of recent advancements in molecular docking techniques for targeting protein misfolding in neurodegenerative diseases. We discuss the principles and methodologies behind molecular docking, including various scoring functions and algorithms employed for accurate ligand-protein interactions. Additionally, we explore the use of molecular dynamics simulations and machine learning approaches to enhance the precision of docking studies. Furthermore, we highlight case studies and success stories where molecular docking has contributed to the discovery of potential drug candidates for neurodegenerative diseases. These include compounds that inhibit amyloid-β aggregation in Alzheimer's disease, α-synuclein oligomerisation in Parkinson's disease, and mutant huntingtin aggregation in Huntington's disease. We also discuss the problems and restrictions of molecular docking related to neurodegenerative diseases, such as how to accurately show the flexibility of proteins and why docking results need to be confirmed by experiments. We also discuss the structural biology methods, such as cryo-electron microscopy and X-ray crystallography, and how these techniques might help in improving molecular docking studies.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143457161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on Precision Medicine AI Algorithm for Neuro Immune Gastrointestinal Diseases based on Quantum Biochemistry and Computational Cancer Genetics.","authors":"Liangyu Li, Bingyao Li, Guangwen Wang, Siyi Li, Xudong Li, Javier Santos, Ana María González, Lizhong Guo, Yanyang Tu, Yi Qin","doi":"10.2174/0113892010348489241210060447","DOIUrl":"https://doi.org/10.2174/0113892010348489241210060447","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study is to conduct network toxicology analysis based on smoking habits and develop a simpler and more effective toxicology product ingestion control system.</p><p><strong>Background: </strong>Smoking behavior can affect the pathogenesis and prognosis of neuroimmune gastrointestinal diseases.</p><p><strong>Aims: </strong>The purpose of developing tools to assist clinical practice is to avoid the harm of cigarettes to the human body.</p><p><strong>Methods: </strong>Molecular dynamics method was used to elucidate the biophysical mechanism of TP53 gene mutation caused by harmful ingredients, and the signaling pathway of midbrain edge excitation was determined by molecular dynamics of nicotine and dopamine receptor D3. The possible involvement of nicotine in neuronal damage was determined through the molecular interaction between nicotine and ACHE. Molecular pathways were analyzed based on the aforementioned biological principles, developed artificial intelligence systems and brain computer interface systems.</p><p><strong>Results: </strong>Several signaling pathways were elucidated, and effective AI algorithms were developed.</p><p><strong>Conclusion: </strong>The accuracy of artificial intelligence systems is over 70%. This study provides clinical doctors with a new precision medicine strategy and tool to regulate patient behavior and reduce disease risk. Other: This project was approved by the Ethics Committee of Chifeng Cancer Hospital and reported to the WHO.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143457165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro Antibiofilm Activity-directed In silico Identification of Natural Products Targeting Bacterial Biofilm Regulators SarA and LasR.","authors":"Jamil A Shilpi, Dipto Kumer Sarker, Veronique Seidel, Mohammad Tuhin Ali, Shaikh Jamal Uddin, Ananya Basak, Shaown Chakraborty, M Khairuzzaman, Akhlak Un Nahar, Fayad Bin Abdus Salam","doi":"10.2174/0113892010348855241113031323","DOIUrl":"https://doi.org/10.2174/0113892010348855241113031323","url":null,"abstract":"<p><strong>Background: </strong>Antibiofilm agents serve as an essential tool in the fight against antibiotic resistance, and natural products provide a promising source for potential drug leads.</p><p><strong>Objective: </strong>This study investigates the activity of twenty Bangladeshi medicinal plants against Staphylococcus aureus and Pseudomonas aeruginosa biofilms and predicts the interactions of selected phytochemicals from five of the best performing plants with the active sites of transcriptional regulatory proteins SarA of S. aureus and LasR of P. aeruginosa.</p><p><strong>Methods: </strong>The plant extracts were tested by microtiter plate-based assay against S. aureus and P. aeruginosa biofilms. Molecular docking and molecular dynamics simulation (MD) were conducted using PyRx and GROMACS, respectively.</p><p><strong>Results: </strong>The best activity was identified for Cassia fistula and Ananas comosus, showing ≥ 75% inhibition of biofilm formation. ent-Epicatechin-(4α→8)-epiafzelechin (EEE) of C. fistula, cyanidin-3,3',5-tri-O-β-D-glucopyranoside (CTG) of A. comosus, and 7-O-(4-hydroxy-Ecinnamoyl)- spinoside of A. spinosus showed the best predictive binding affinity (-7.6, -7.6 and - 7.7 kcal/mol, respectively) for SarA. EEE was the only ligand to exhibit a stable ligand-protein complex with SarA in the MD simulation of 200 ns (binding energy of MMPBSA analysis - 39.899 kJ/mol). Chrysophanol, epicatechin and physcion, of C. fistula (-10.5, -10.5, and -11.0 kcal/mol, respectively) and auraptene of F. limonia (-10.8 kcal/mol) showed the best predictive binding affinity for LasR. Epicatechin showed the most stable ligand-protein complex with LasR (binding energy of MMPBSA analysis -63.717 kJ/mol).</p><p><strong>Conclusion: </strong>Epicatechin and its derivative EEE could be used as scaffolds for the d evelopment of new antibiofilm agents against P. aeruginosa and S. aureus, respectively.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143457162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Double-blind Randomized Split-mouth Clinical Trial on a Hemostatic Dental Material Containing Aloe Vera Nanoparticles: The Effects on Pain and Occurrence of Dry Socket after Tooth Extraction.","authors":"Samin Baghban Vazirabadi, Mahsa Mehrpouya, Naiemeh Motallebi, Olduz Hosseini, Ali Zaheri Khosroshahi, Simin Sharifi, Solmaz Maleki Dizaj, Mohammad Ali Ghavimi","doi":"10.2174/0113892010363882250210054655","DOIUrl":"https://doi.org/10.2174/0113892010363882250210054655","url":null,"abstract":"<p><strong>Introduction: </strong>Pain, infection, and dry socket are the primary complications following tooth extraction. Hence, the aim of this study was to evaluate the pain range and incidence of dry socket after using a hemostatic spongy material containing aloe vera nanoparticles after split-mouth extraction of the mandibular first and second molars, compared to a control sponge without aloe vera nanoparticles in patients referred to the Tabriz Faculty of Dentistry.</p><p><strong>Materials and methods: </strong>This double-blind, split-mouth clinical study was conducted on 30 patients referred to the Faculty of Dentistry in Tabriz. Patients were selected according to specific inclusion and exclusion criteria and required extraction of two teeth. After tooth extraction, an aloe vera nanoparticle-containing hemostatic sponge was randomly placed in one extraction site, while a sponge without aloe vera nanoparticles served as the control in the other extraction site. Pain levels were measured at 12, 24, 48, and 72 h after extraction using a Visual Analog Scale (VAS), where patients marked their pain level from 1 (lowest pain) to 10 (highest pain) at each time point. Patients received a form to record the required information and instructions on how to do so, which they submitted upon completion. To assess the occurrence of dry socket, patients were asked to come four days after extraction. Data were analyzed using SPSS software with relevant statistical tests, considering a significance level of P<0.05.</p><p><strong>Results: </strong>In both groups, the average pain levels decreased significantly over 72 h (P=0.001 and P=0.01 for the test and control groups, respectively). Additionally, for 48 h and 72 h time points, the test group showed better pain control results than the control group (P=0.04 and P=0.04 for 48 h and 72 h, respectively). No cases of dry sockets were found in either group.</p><p><strong>Conclusion: </strong>The aloe vera-containing sponge demonstrated better performance in pain control following tooth extraction compared to the control group. For dry socket occurrence, both sponges showed similar results.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143413694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}