Current pharmaceutical biotechnology最新文献

{"title":"Suppression of Quorum-sensing-regulated Virulence and Biofilm Formation in Pseudomonas aeruginosa by the Extract of Cichorium intybus Root.","authors":"Atefeh Zolfaghari, Mohammad Javad Mehdipour Moghaddam, Akbar Norastehnia","doi":"10.2174/0113892010442625260130062234","DOIUrl":"https://doi.org/10.2174/0113892010442625260130062234","url":null,"abstract":"<p><strong>Introduction: </strong>The rising prevalence of antibiotic-resistant Pseudomonas aeruginosa underscores the urgent need for new antimicrobial approaches. This pathogen forms biofilms and produces quorum-sensing (QS)-regulated factors that aid its survival and resistance. This study investigates the antimicrobial, antibiofilm, and QS gene expression effects of Cichorium intybus root extracts on clinical P. aeruginosa isolates.</p><p><strong>Methods: </strong>Ethanolic and aqueous extracts of C. intybus were prepared and analyzed for phenolic, flavonoid, flavonol, and anthocyanin content. Antioxidant activity was assessed using the DPPH assay. Antibacterial activity was evaluated using well diffusion, MIC, and MBIC assays. Biofilm formation was quantified using crystal violet staining, and QS gene expression (lasR, lasI, lasB) was analyzed by qRT-PCR.</p><p><strong>Results: </strong>The aqueous extract had higher phenolic content, whereas the ethanolic extract contained more flavonoids and flavonols (p<0.05). Only strain PA3 was sensitive to the extracts. The ethanolic extract produced larger inhibition zones (15 mm vs. 13 mm, p<0.05), had a MIC of 0.048 g/ml, and significantly inhibited biofilm formation at 0.024 g/ml (p<0.05). qRT-PCR results indicated decreases in lasI, lasR, and lasB gene expression by 23%, 39.9%, and 35.7%, respectively.</p><p><strong>Discussion: </strong>These results suggest that C. intybus root extracts, particularly ethanolic extracts, can suppress P. aeruginosa growth and biofilm formation while modulating QS-regulated virulence factors. This suggests a promising alternative to conventional antibiotics.</p><p><strong>Conclusion: </strong>The ethanolic extract of C. intybus root exhibits notable antimicrobial, antibiofilm, and antivirulence activity, highlighting its promise as a treatment option.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147834735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety Assessment of Probiotics and Postbiotics: A Critical Review.","authors":"Samira Ranjbar, Reza Ghotaslou, Khudaverdi Ganbarov, Şükran Köse, Amin Abbasi, Hossein Samadi Kafil","doi":"10.2174/0113892010419857251208064650","DOIUrl":"https://doi.org/10.2174/0113892010419857251208064650","url":null,"abstract":"<p><p>Probiotics and postbiotics are essential components of modern dietary supplements, recognized for their beneficial effects on human health. While probiotics positively influence gut microbiota, digestion, and immune responses, their safety remains a concern, particularly in immunocompromised individuals, due to risks such as infections, transfer of antibiotic resistance genes, and systemic complications. In contrast, postbiotics-defined as inanimate microorganisms and/or their cellular components-offer a safer alternative by providing similar health benefits without the risks associated with live microorganisms. However, safety assessments of postbiotics remain limited and must address critical issues such as cytotoxicity, impurity profiles, and dose-response uncertainties, which are currently underexplored in the literature. Their enhanced stability under various environmental conditions further supports their application in inhibiting foodborne pathogens and extending product shelf life. This review critically evaluates the safety profiles of probiotics and postbiotics, offering a balanced analysis of their benefits and limitations, with particular attention to emerging safety concerns, risk assessment criteria, and applications in food safety and human health. Despite their promising safety profile, widespread adoption of postbiotics is hindered by the absence of standardized safety protocols and long-term toxicological data. Future research should therefore prioritize establishing evidence-based safety criteria, optimizing dosage and formulation for both probiotics and postbiotics, and addressing existing knowledge gaps through rigorous, targeted clinical studies, especially in vulnerable populations. A comprehensive literature search was conducted using the ISI Web of Knowledge, PubMed, Medline, Embase, Cochrane Register of Controlled Trials (CENTRAL), and Scopus databases covering the period from 2000 to 2025.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147834695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydroxysafflor Yellow A Attenuates Myocardial Ischemia/Reperfusion Injury-induced Platelet Activation: Role of Mitochondrial Function and Autophagy.","authors":"Yaqi Zhang, Zhihui Wang, Ruotong Li, Zhiwei Liu, Rui Zhang, Ruoyun Li, Min Wang, Guibo Sun","doi":"10.2174/0113892010470039260325123335","DOIUrl":"https://doi.org/10.2174/0113892010470039260325123335","url":null,"abstract":"<p><strong>Introduction: </strong>Platelet activation is a key contributor to myocardial ischemia/ reperfusion (MI/R) injury. Although hydroxysafflor yellow A (HSYA) possesses cardioprotective and antiplatelet properties, its specific mechanisms in modulating platelet activation during MI/R remain unclear.</p><p><strong>Objectives: </strong>This study investigated how HSYA attenuates MI/R injury by regulating platelet activation.</p><p><strong>Methods: </strong>An MI/R model was established in mice via ligation of the left anterior descending coronary artery. The regulatory effects of HSYA on platelet activation and mitochondrial function were evaluated via flow cytometry. The mechanisms were analyzed via platelet proteomics. Platelet autophagy was characterized via transmission electron microscopy and western blotting.</p><p><strong>Results: </strong>HSYA significantly attenuated MI/R injury by reducing the myocardial infarct size, improving cardiac function, and decreasing platelet accumulation in the myocardium. HSYA also suppressed platelet activation and enhanced platelet mitochondrial function. Platelet proteomic analysis indicated that the beneficial effects of HSYA were associated primarily with the modulation of proteins involved in energy metabolism and autophagy pathways. Furthermore, HSYA was found to regulate platelet autophagy, as evidenced by both a change in the number of autophagosomes and altered expression of key autophagy-related proteins, including ATG4A, GLIPR2, P62, and LC3.</p><p><strong>Discussion: </strong>Our study provides novel mechanistic insights into how HSYA confers cardioprotection against MI/R injury, highlighting its clinical potential by demonstrating that its effects are mediated at least in part through the modulation of platelet energy metabolism and autophagy.</p><p><strong>Conclusion: </strong>HSYA alleviates MI/R injury by inhibiting platelet activation, which is associated with the restoration of mitochondrial function and the modulation of autophagy in platelets.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147764710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitigation of Cadmium-induced Renal Damage by Meso-2,3-Dimercaptosuccinic Acid and Spirulina: A Comparative Study.","authors":"Heba H Tarabay, Abdel-Aziz A F, Manar E Elkady, Hazem H Saleh, Mahmoud Abdelgawad, Bedeir Ali-El-Dein","doi":"10.2174/0113892010429980251223102626","DOIUrl":"https://doi.org/10.2174/0113892010429980251223102626","url":null,"abstract":"<p><strong>Introduction: </strong>Industrial expansion increases human exposure to Cadmium (Cd), a heavy metal that accumulates in the kidneys, causing tubular injury and chronic kidney disease. This study evaluates the chelating effects of meso-2,3-dimercaptosuccinic acid (DMSA) and spirulina's protective role against Cd-induced nephrotoxicity in rats.</p><p><strong>Methods: </strong>Forty male Sprague-Dawley rats (6-7 weeks old, 200-250 g) were randomly divided into five groups (n = 8/group): (1) Control group received distilled water; (2) Cd group received cadmium chloride (5 mg/kg body weight) orally, three times/week for 6 weeks; (3) Cd + DMSA group received DMSA (50 mg/kg) orally, daily for 2 weeks following Cd exposure; (4) Spirulina treatment group received spirulina (1 g/kg) orally, daily alongside Cd; (5) Spirulina protection group received spirulina 2 weeks before and throughout Cd exposure. Renal function markers, oxidative stress indicators (8-OHdG, isoprostane, MDA), antioxidant enzymes (GSH, SOD, CAT), and gene expression of inflammatory (TNF-α, NF-κB), apoptotic (Bax), anti-apoptotic (BCL-2), and detoxification (MT1) markers were assessed. Data were analyzed using one-way ANOVA followed by Tukey's post hoc test, with significance set at p < 0.05.</p><p><strong>Results: </strong>Cd exposure significantly impaired renal function, increased oxidative stress, and upregulated pro-inflammatory and apoptotic markers (p < 0.05). Both DMSA and spirulina significantly mitigated these effects, with prophylactic spirulina showing the most significant improvements.</p><p><strong>Discussion: </strong>Administering DMSA and spirulina effectively mitigates Cd-induced nephrotoxicity and helps restore normal kidney function.</p><p><strong>Conclusion: </strong>Spirulina and DMSA demonstrate protective effects against cadmium-induced nephrotoxicity. Spirulina, particularly when used prophylactically, potentially offering superior renal protection.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Onco-lipidomics: Unraveling Potential Biomarkers for Breast Cancer Progression and their Pharmacological Relevance.","authors":"Suman Kumar Ray, Sukhes Mukherjee","doi":"10.2174/0113892010433897251208064543","DOIUrl":"https://doi.org/10.2174/0113892010433897251208064543","url":null,"abstract":"<p><p>Breast cancer is the most common type of cancer in women, accounting for around 10% of all occurrences. Most cancer patients die because of metastasis, which is now untreatable. Despite this, there are several ways that can be used to reduce the intensity of breast cancer symptoms. This emphasizes the need to start breast cancer treatment and diagnosis as soon as possible to prevent the disease from spreading. Mammography and ultrasounds are useful for screening many people, but they are ineffective at identifying and grading breast cancer. However, they are useful for detecting breast cancer. Doctors continue to do these kinds of procedures. Advanced biomarker development has the potential to transform several parts of medicine. Some of these characteristics include early illness detection, disease progression monitoring, and the development of treatment programs for specific tumors. Patients have a better quality of life and suffer less harm because of obtaining more effective therapies in moderation. As a result, lipidomic and metabolomic signatures are emerging as promising biomarkers for improving breast cancer detection and treatment. The development of lipidomics was driven by these objectives. The analytical power of mass spectrometry and the lightning-fast pace of technological progress have enabled lipidomics to make significant strides in recent years. By discussing onco-lipidomics and other important cellular metabolic networks, as well as drug-related issues in breast cancer, this review aims to provide readers with the knowledge they need to get a deeper understanding of metabolism.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147671046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Mpox Outbreak 2025: A Call for Coordinated Global Action and Equity.","authors":"Sachin Yadav, Shivangi, Yashika, Anish Arora, Komal Kishor, Ratnesh Kumar Yadav, Raj Kumar Narang, Amandeep Singh","doi":"10.2174/0113892010467611260306053153","DOIUrl":"https://doi.org/10.2174/0113892010467611260306053153","url":null,"abstract":"<p><p>The multi-country Mpox (monkeypox) outbreak remains an important issue of global public health, with reported cases of 44,299 and 180 deaths, respectively, in 93 countries by September 2025. Although Africa remains the epicentre, with almost 40,000 cases within countries such as the Democratic Republic of the Congo (DRC), Liberia, Kenya, and Ghana, Europe and South-East Asia have also become new hotspots due to the growing clade Ib virus strain. It is a strain that spreads mostly among men who have sex with men, creating new difficulties because of declining vaccine immunity and the influx of immunologically naive groups. The World Health Organization (WHO) and the Africa Centres for Disease Control and Prevention (Africa CDC) are collaborating to implement robust surveillance, vaccination, and clinical care activities. To date, more than 1.16 million doses of vaccine have been delivered in Africa, utilising dose-sparing measures. Constant loopholes remain, such as contact tracing, resource insufficiency, and data latencies. The vision of the future requires increased vaccination, combined with Human Immunodeficiency Virus (HIV)/Sexually Transmitted Infection (STI) services, improved genomic surveillance, and increased global collaboration. Constant observation, reactionary measures, and fair resource distribution are crucial in preventing transmission and protecting at-risk groups.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147618501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative Utilization of Endolysins for Future Battle Against Superbugs.","authors":"Ping Zeng","doi":"10.2174/0113892010450500260226080647","DOIUrl":"https://doi.org/10.2174/0113892010450500260226080647","url":null,"abstract":"<p><p>Endolysins are fast-acting proteins produced by phages against bacteria, capable of breaking down the peptidoglycan layer of bacterial cells. In the current post-antibiotic era, endolysin-based therapeutics have received widespread attention. While native endolysins offer advantages such as rapid bacteriolytic activity, their clinical translation has been hindered by inherent limitations, including narrow spectrum, susceptibility to proteolytic degradation, and potential immunogenicity. Recent paradigm shifts in protein engineering and biotechnology are decisively overcoming these hurdles. This perspective highlights the transformative strategies revolutionizing the field, such as the creation of chimeric endolysins fused with outer membrane-permeabilizing peptides and the rational modulation of protein surface charge. Beyond that, endolysins demonstrate remarkable synergy with conventional antimicrobial agents such as colistin and berberine, enabling dose reductions and resensitizing resistant strains. Their application scope is broadening into areas such as food safety, where they effectively combat biofilms and multidrug-resistant pathogens across various food matrices. Moreover, critical challenges of stability and delivery are being addressed through advanced formulations. Endolysins are poised to evolve from a promising candidate into an innovative medical strategy against superbugs.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147607876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trichosanthes pericarpium water fraction prevents myocardial cells from apoptosis induced by ox-LDL via increasing its sensitivity to FGF21.","authors":"Teng Sun, Chaoqun Hu, Haiyu Wang, Sijie Li, Qing Wei, Qitao Zhao","doi":"10.2174/0113892010387606260105110739","DOIUrl":"https://doi.org/10.2174/0113892010387606260105110739","url":null,"abstract":"<p><strong>Background: </strong>The traditional Chinese medicine (TCM) Trichosanthes pericarpium (TP) exerts uniquely therapeutic effect on a TCM syndrome of coronary heart disease, the phlegm turbid obstruction syndrome, the primary characteristic of which is severe lipid metabolism disorder. However, the mechanism underlying the remains elusive.</p><p><strong>Objective: </strong>We speculated that protecting heart from damaged by hyperlipidemia via evoking fibroblast growth factor 21 (FGF21) signaling may partly account for above mechanism.</p><p><strong>Methods: </strong>Rat primary myocardial cells (MCs) was isolated from the heart of neonatal rats, and were exposure to oxidized low density lipoprotein (ox-LDL), together with or without the water fraction (TP-W), the effective constituents of TP.</p><p><strong>Results: </strong>Severe apoptosis was could be observed in MCs exposure to ox-LDL, meanwhile, the level of FGF21 in cells and medium increased significantly; however, the upregulation of βKlotho expression was suppressed dramatically, fibroblast receptor substrate 2α (p-FRS2α) could not be adequately phosphorylated too. In cells incubated with both ox-LDL and TP-W, the expression of FGF21 was accelerated notably; moreover, the level of βKlotho and p-FRS2α was promoted memorably while the apoptosis was reduced signally.</p><p><strong>Conclusions: </strong>TP-W could rescue the apoptosis stimulated by ox-LDL via activating FGF21 signaling, restoring its sensitivity to FGF21 in MCs.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147493733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Probiotics in Oral Health: A Review From Microbial Balance to Clinical Applications.","authors":"Jiang Foong Kong, Hiu Ching Phang, Wan Hamirul Bahrin Wan Kamal, Yen Ng, Saifullizan Mohamad, Phei Er Kee, Kai Bin Liew","doi":"10.2174/0113892010421039251206192855","DOIUrl":"https://doi.org/10.2174/0113892010421039251206192855","url":null,"abstract":"<p><p>A diverse microbial community exists within the human oral cavity that plays an essential role in maintaining health or inducing diseases such as dental caries, periodontal disease, and halitosis. Probiotics, live microorganisms that provide health benefits when consumed in adequate amounts, have been found to be promising as a means of modulating the oral microbiome and combating these diseases. This review incorporates present knowledge about the mechanism of probiotic action, including competitive exclusion of pathogens, antimicrobial metabolite production, biofilm disruption, and immune modulation. Efficacy against pathogenic bacteria like Streptococcus mutans and Porphyromonas gingivalis has been proven by prominent probiotic groups Lactobacillus, Bifidobacterium, and Streptococcus, resulting in oral microbial homeostasis. Clinical applications of probiotics include prevention of caries, plaque reduction, and management of gingivitis and periodontitis, with research focusing on strain-specific effects. Emerging trends include precision probiotics tailored to each oral condition, postbiotics as strong alternatives (formerly \"strong contenders\"), and innovative delivery systems to enhance viability and colonization. The hurdles of strain specificity, regulatory gaps, and inconsistencies of clinical outcome continue. Safety concerns, while rare, represent possible risks of horizontal gene transfer and opportunistic infections in immunocompromised hosts. Future directions lie in genetic modification, new delivery methods, and standard clinical protocols to enhance probiotic function. This review emphasizes the clinical potential of probiotics as adjunctive treatments in oral medicine, with the caveat that further work is needed to overcome current challenges and enhance their therapeutic efficacy.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated In silico and In vitro Screening of Phytoconstituents as Potential Inhibitors of New Delhi Metallo-β-lactamase-1 (NDM-1).","authors":"Ajay Kumar, Sapna Redhu, Ajmer Singh Grewal, Suresh Kumar Gahlawat","doi":"10.2174/0113892010419139251205144039","DOIUrl":"https://doi.org/10.2174/0113892010419139251205144039","url":null,"abstract":"<p><strong>Introduction/objective: </strong>The emergence of New Delhi metallo-β-lactamase-1 (NDM- 1) in Escherichia coli has become a significant public health threat due to its ability to hydrolyze a broad range of β-lactam antibiotics, including carbapenems. This study aimed to identify natural inhibitors of NDM-1 using a combined in silico and in vitro approach, with a focus on phytoconstituents from medicinal plants.</p><p><strong>Methods: </strong>Five medicinal plants were screened for antibacterial activity against an NDM-1- producing E. coli strain. Extracts of Curcuma longa L. prepared with acetone, ethanol, and water were evaluated using an agar well diffusion assay. A phytochemical database of C. longa was compiled, and 144 compounds were assessed for drug-likeness using SwissADME. Compounds meeting selection criteria were subjected to molecular docking against the NDM-1 protein. The top hit was further evaluated for antibacterial and synergistic activity with amoxicillin. Minimum Inhibitory Concentration (MIC) was determined, and in silico toxicity and pharmacokinetic profiles were predicted.</p><p><strong>Results: </strong>C. longa acetone extract exhibited the highest antibacterial activity, with inhibition zones ranging from 8 to 16 mm. Ninety-six compounds passed drug-likeness filters; bisdesmethoxycurcumin showed the best binding energy (-6.5 kcal/mol) with NDM-1. In vitro, it demonstrated synergistic activity with amoxicillin, increasing inhibition zones from 10.33 mm to 19.67 mm. MIC was found to be 25-30 μg/ml. Toxicity predictions suggested acceptable safety and pharmacokinetic parameters.</p><p><strong>Discussion: </strong>The study highlights the potential of bis-desmethoxycurcumin as an adjuvant to conventional antibiotics to overcome NDM-1-mediated resistance.</p><p><strong>Conclusion: </strong>Bis-desmethoxycurcumin from C. longa exhibits promising inhibitory activity against NDM-1-producing E. coli, supporting its potential use in adjuvant therapy to combat antibiotic resistance.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147456054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}