Current pharmaceutical biotechnology最新文献

{"title":"Nutritional Evaluation and Free Radical Scavenging Activity of Nano-formulated Selenium-Moringa Peregrine Seed Extract as a Promising Suppressor of TGF-β1/P38/NF-kβ Signaling Pathway in HgCl2 Intoxicated-Mice.","authors":"Mahmoud M Eltawila, Reham A Hamdy, Mohammed A Hussein, Samar M Aborhyem","doi":"10.2174/0113892010352642241221152820","DOIUrl":"10.2174/0113892010352642241221152820","url":null,"abstract":"<p><strong>Background: </strong>Moringa peregrina, renowned for its extensive health benefits, continues to reveal its therapeutic potential through ongoing research. The synthesis of Moringa peregrina extract-selenium nanoparticles (MPE-SeNPs) has emerged as a promising approach in developing versatile therapeutic agents.</p><p><strong>Objective: </strong>To evaluate the protective effects of MPE-SeNPs against oxidative damage and inflammation caused by HgCl2 exposure in mice.</p><p><strong>Methods: </strong>The study involved extracting and characterizing the dried powdered seeds of Moringa peregrina to determine their nutritional and bioactive contents. MPE-SeNPs were synthesized using plant extracts and characterized through TEM, UV-Vis, and FT-IR techniques to assess their chelating and superoxide radical scavenging activities. The LD50 of MPE-SeNPs was determined, and doses of 1/50 and 1/20 of the LD50 were administered to HgCl₂-exposed mice to evaluate lung protective effects. Biochemical analyses measured plasma lipid profiles and lung antioxidant status, while gene expression of TGF-β1, P38, and NF-kβ in lung tissue was analyzed. Histopathological examinations of lung tissues were conducted to observe structural changes and fibrosis, providing a comprehensive assessment of the protective efficacy of MPE-SeNPs against oxidative damage and inflammation.</p><p><strong>Results: </strong>The raw Moringa peregrine seeds contain approximately 27.81% fat, 32.10% protein, 13.11% fiber, 4.11% ash and 22.93% carbohydrate content. The phenolic and flavonoid content in debittered seeds is approximately 76.42 mg of GAE/g DE), and 15.55 mg of QE/g DE, respectively. However, MPE and MPESeNPs exhibited chelating activity with 54 and 80.64% after 60 min. Additionally, at a concentration of 120 μg/mL, the superoxide radical scavenging activity was 71% for MPE and 93% for MPE-SeNPs after 5 minutes of incubation. The IC50 values recorded for MPE and MPE-SeNPs were 80.38 and 48.01 μg/mL, respectively. MPE-SeNPs had an average size of approximately 130.63 nm. UV-Vis spectrum peaks and FTIR identified functional groups associated with phenolics and flavonoids. LD50 of MPE-SeNPs was estimated to be 773 mg/kg body weight. Oral administration of MPE and MPE-SeNPs led to improvements in plasma lipid profile as well as lung antioxidant status. Moreover, downregulation of lung TGF-β1, P38, and NF-kβ gene expression in HgCl2-intoxicated mice when treated with MPE-SeNPs. In addition, MPE-SeNPs improve lung tissue by enhancing antioxidant enzymes, suppressing pro-inflammatory cytokines, and scavenging free radicals.</p><p><strong>Conclusion: </strong>The study reveals that Moringa peregrina extract combined with selenium nanoparticles (MPESeNPs) offers significant protection against oxidative damage induced by HgCl₂ exposure. The enhanced antioxidant and anti-inflammatory properties of MPE-SeNPs, particularly at a dose of 38.65 mg/kg body weight, demonstrate their poten","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticles as Delivery Vehicles for Vaccines: The Use of Gold Nanoparticles.","authors":"Nadeem Kizilbash, Nida Suhail, Mohamed Soliman, Ranya Mohammed Elmagzoub, Madison Marsh, Rimsha Farooq","doi":"10.2174/0113892010363803250110052220","DOIUrl":"https://doi.org/10.2174/0113892010363803250110052220","url":null,"abstract":"<p><p>Since their inception, therapeutic or prophylactic vaccines have emerged as promising candidates for the prevention or treatment of infections and various diseases, including cancer and autoimmune disorders. In recent times, gold nanoparticles (GNPs) have acquired active roles in the field of vaccine development due to their intrinsic capacity to adjust and enhance the immune response. Due to their characteristics, GNPs can exert optimal effects as both delivery vehicles and adjuvants. Despite their significant importance in vaccinology, numerous obstacles need to be overcome before GNPs can be used in the formulations of vaccines in clinical settings. The current review summarizes the latest and successful use of gold nanoparticles as a viable method for developing a new generation of vaccines.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Niosomal Encapsulation of Anti-Cancer Peptides: A Revolutionary Strategy in Cancer Therapy.","authors":"Bhaveshkumar A Patel, Ankur Vashi, Ramakrishna Borra, Mehul Patel","doi":"10.2174/0113892010344931241220042746","DOIUrl":"https://doi.org/10.2174/0113892010344931241220042746","url":null,"abstract":"<p><p>Cancer treatment has evolved significantly over the years, incorporating a range of modalities including surgery, radiation, chemotherapy, and immunotherapy. However, challenges such as drug resistance, systemic toxicity, and poor targeting necessitate innovative approaches. Peptides have gained attention in cancer therapy due to their specificity, potency, and ability to modulate various biological pathways. Peptide-based drugs can act as hormones, enzyme inhibitors, or targeting ligands, contributing to their versatile role in cancer treatment. However, peptides face several challenges, including instability, rapid degradation, and poor bioavailability. One promising strategy is the use of niosomal delivery systems for peptidebased therapies. Niosomes, which resemble liposomes in structure, are vesicles based on nonionic surfactants. They are composed of a bilayer created through the self-assembly of non-ionic surfactants in water, enabling them to encapsulate hydrophilic, lipophilic, and amphiphilic drugs. Their unique properties, such as biocompatibility, biodegradability, and ability to encapsulate diverse therapeutic agents, make them suitable for drug delivery applications. This review aims to explore how the niosomal preparation of peptides can revolutionize oncology drugs by overcoming critical challenges like drug resistance, systemic toxicity, poor targeting, instability, rapid degradation, and low bioavailability. This review aims to explore how niosomes can specifically address key limitations in cancer therapy, including targeting, bioavailability, and stability of peptide-based drugs. By consolidating recent advancements, the review sheds light on how niosomal encapsulation can overcome barriers in cancer treatment and improve therapeutic outcomes for patients.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological Perspectives on the Therapeutic Potential of Phenylpropanoid Cinnamaldehyde in Inflammatory Diseases: Signaling Pathways.","authors":"Ian Jhemes Oliveira Sousa, Kerolayne De Melo Nogueira, Rita de Cássia Meneses Oliveira","doi":"10.2174/0113892010343782250110113310","DOIUrl":"https://doi.org/10.2174/0113892010343782250110113310","url":null,"abstract":"<p><p>The traditional use of plants of the Cinnamomum genus dates back to traditional Eastern medicine for millennia and they have also been used in Western integrative medicine practices, especially for their anti-inflammatory activity. In the context of chemical diversity, the absolute majority of species in this genus have cinnamaldehyde as the majority component, which in turn holds the title of the active ingredient, whose biological effect profile has already been demonstrated in numerous experiments in acute and chronic inflammatory conditions. In this context, the objective of this research was to investigate how cinnamaldehyde can influence inflammatory phenomena. To this end, the data search was carried out in Pub- Med, SciELO, and LILACS databases using the following descriptors: \"cinnamaldehyde OR cinnamaldehyde\" and \"inflammation\". Next, the methodological quality of the selected articles was checked, excluding repeated studies, which were purely in silico and not relevant to the construction of this research. The findings showed cinnamaldehyde to modulate the inflammatory response by inhibiting pro-inflammatory mediators and important signaling trajectories, including NF-κB, NLRP3 inflammasome, TGF-β/Smads, and the NRF2/ARE antioxidant pathway. Cinnamaldehyde's scope of investigation also involves aspects of translational science, exhibiting therapeutic effects in human models, which suggests that it may have clinical relevance. The data support that cinnamaldehyde is a promising candidate for development as a treatment in inflammatory pathologies.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lutein and Zeaxanthin: Source, Extraction, Stability, Bioactivity, and Functional Food Applications.","authors":"Surabhi Pandey, Akansha Gupta, Dipendra Kumar Mahato, Veena Paul, Abhishek Dutt Tripathi, Prasad Rasane, Pradeep Kumar, Madhu Kamle, Shafiul Haque","doi":"10.2174/0113892010334209241206113640","DOIUrl":"10.2174/0113892010334209241206113640","url":null,"abstract":"<p><p>Nature has been acknowledged as a fundamental source of diverse bioactive molecules. Among natural carotenoids, lutein, zeaxanthin, and their oxidative metabolites are specifically deposited in the macular region of living organisms. Lutein and zeaxanthin are carotenoids primarily found in green leafy vegetables, eggs, and various fruits. Lutein and zeaxanthin emerge as the primary carotenoids in the retina, playing a crucial role as photo-protectants to prevent retinal degeneration. The extraction of lutein and zeaxanthin from natural sources involves several techniques, including solvent extraction and supercritical fluid extraction, with an emphasis on optimizing efficiency and yield. Incorporating these carotenoids into functional foods-such as fortified dairy products, baked goods, and snacks-enhances their nutritional profiles and provides significant health benefits. This review examines the sources, stability, bioactivity, and various extraction methods for lutein and zeaxanthin, highlighting their potential for photoprotection, antioxidant activity, and antidiabetic effects. These attributes, combined with innovative extraction techniques, position lutein and zeaxanthin as promising ingredients for functional food applications.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Roadmap Towards the Identification and Validation of Conserved T Cell Epitope Regions in Viral Pathogen Families with Pandemic Potential.","authors":"Alba Grifoni, John Sidney, Daniela Weiskopf, Richard H Scheuermann, Alessandro Sette","doi":"10.2174/0113892010349200241204062202","DOIUrl":"https://doi.org/10.2174/0113892010349200241204062202","url":null,"abstract":"<p><p>The SARS-CoV-2 pandemic has highlighted the need for society, as a whole, to be prepared against potential pandemics caused by a variety of different viral families of concern. Here, we describe a roadmap towards the identification and validation of conserved T cell epitope regions from Viral Families of Pandemic Potential (VFPP). For each viral family, we select a prototype virus, the sequence of which could be utilized in epitope identification screens. Examples of viral families considered and their respective prototypes (species/ subspecies) are Coronaviridae (Severe Acute Respiratory Syndrome-related Coronavirus/ SARS-CoV-2), Flaviviridae (Dengue virus/DENV2), Togaviridae (Chikungunya virus/ CHIKV), Paramyixoviridae (Morbillivirus/measles), Arenaviridae (Mammarenavirus/ Lassa), and Picornaviridae (Enterovirus C/poliovirus). The peptide sequences encoded in each prototype virus are then analyzed to determine their conservation across different viral taxonomic groups and viral variants derived from each of the VFPP. We outline available methodologies for epitope discovery based on panels of overlapping peptides and bioinformatics- based predictions of HLA-peptide binding, along with high-throughput in vitro assays, with emphasis on addressing coverage of the general worldwide population. Validation can be achieved by a variety of methodologies, including determining HLA restriction and recognition in samples from volunteers convalescent from previous infections or immunized with approved or experimental vaccines, and immunophenotyping of responding T cells. The capacity of these regions to induce crossreactive T cell responses can be tested experimentally with homologous peptides derived from the various viral species of interest. Importantly, they could be considered as a component of pan-viral family vaccines. Conversely, immunogenic regions that are highly specific to a given virus could be of interest for diagnostic applications.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"\"Skin Whitening Creams: Cosmetovigilance.\"","authors":"Saloni Agarwal, Mohd Mazhar, Hiranmoy Saha, Simra Khan, Mohammad Ibrahim, Charu Chhabra","doi":"10.2174/0113892010323719241205045837","DOIUrl":"https://doi.org/10.2174/0113892010323719241205045837","url":null,"abstract":"<p><p>Cosmeceutical products such as skin-lightening agents have been used globally to enhance skin tone and obtain a magnificent outward appearance. The pigment known as melanin, produced by melanocytes, imparts skin color. The Cosmetic Europe survey testifies that most people believe that cosmetics enhance one's quality of life. The most in-demand in the cosmetics industry are skin-whitening agents. Heavy metals and dangerous substances like lead and mercury are present in these skin-brightening cosmetics, which have a long-term effect on important organs and begin asymptomatic. Despite these negative consequences, there is a sizable market for these products due to the desire for fairer skin tones. Cosmetovigilance studies determine the of heavy metals in these preparations, which need to be monitored regularly. This article discusses the toxicity of heavy metals such as nickel, mercury, arsenic, lead, cobalt, and others found in skin-lightening formulas. The acceptable limit for these toxic agents is recognized by several healthcare organizations worldwide.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying Novel Therapeutic Opportunities for Dilated Cardiomyopathy: A Bioinformatics Approach to Drug Repositioning and Herbal Medicine Prediction.","authors":"Jiao Wang, Tianwei Meng, Na Si, Haihong Li, Yan Yan, Xinghua Li","doi":"10.2174/0113892010335576241202061139","DOIUrl":"https://doi.org/10.2174/0113892010335576241202061139","url":null,"abstract":"<p><strong>Background: </strong>Dilated Cardiomyopathy (DCM) is a debilitating cardiovascular disorder that challenges current therapeutic strategies. The exploration of novel drug repositioning opportunities through gene expression analysis offers a promising avenue for discovering effective treatments.</p><p><strong>Objective: </strong>This study aims to identify potential drug repositioning opportunities and lead compounds for DCM treatment by optimizing gene expression characteristics using published data.</p><p><strong>Methods: </strong>Our approach involved analyzing DCM expression profiles from the Gene Expression Omnibus database and identifying differentially expressed genes with GEO2R. A protein interaction network was constructed using the STRING database and visualized with Cytoscape. Enrichment analyses were conducted on these genes through the Omicshare platform, followed by the identification of candidate compounds via the Connectivity Map (CMAP) and validation through molecular docking. The Coremine Medical database was utilized to predict potential herbal medicines.</p><p><strong>Results: </strong>We identified 29 differentially expressed genes, highlighting MYH6, NPPA, and NPPB as central to DCM pathology. Enrichment analyses indicated significant impacts on biological processes, such as organ morphogenesis and inflammatory responses. The AGE-RAGE signaling pathway was notably affected. From over 6,100 compounds analyzed, tenoxicam emerged as a promising candidate, with Radix Salviae Miltiorrhizae (Danshen) being suggested as a potential herbal treatment.</p><p><strong>Conclusion: </strong>This study underscores the utility of bioinformatics in uncovering new therapeutic candidates for DCM, offering a foundational step towards novel drug development.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COL1A1, COL1A2, CHN1, and FN1 Promote Tumorogenesis and Act as Markers of Diagnosis and Survival in Gastric Cancer Patients.","authors":"Yan Yichao, Li Yongbai, Liang Hailiang, Chen Dongbo, Li Bo, Abduh Murshed","doi":"10.2174/0113892010332329241119104430","DOIUrl":"https://doi.org/10.2174/0113892010332329241119104430","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to comprehensively investigate the molecular landscape of gastric cancer (GC) by integrating various bioinformatics tools and experimental validations.</p><p><strong>Methodology: </strong>GSE79973 dataset, limma package, STRING, UALCAN, GEPIA, OncoDB, cBioPortal, DAVID, TISIDB, Gene Set Cancer Analysis (GSCA), tissue samples, RT-qPCR, and cell proliferation assay were employed in this study.</p><p><strong>Results: </strong>Analysis of the GSE79973 dataset identified 300 differentially expressed genes (DEGs), from which COL1A1, COL1A2, CHN1, and FN1 emerged as pivotal hub genes using protein-protein interaction network analysis. Subsequent validation across The Cancer Genome Atlas (TCGA) datasets confirmed their up-regulation in GC tissues compared to normal controls. Promoter methylation analysis revealed decreased methylation levels of these hubs in GC tissues, suggesting their potential role in tumorigenesis. Mutational analysis using cBioPortal showcased frequent mutations in these genes, particularly FN1, further highlighting their significance in GC pathogenesis. Survival analysis indicated their correlation with reduced overall survival rates among GC patients, supported by the development of a robust prognostic model. Prediction of hub-associated miRNAs and gene enrichment analysis provided insights into their regulatory mechanisms and downstream pathways, implicating their involvement in extracellular matrix remodeling and cell migration. Drug sensitivity analysis revealed correlations between hub gene expression and drug response, while RT-qPCR validation confirmed their upregulation in clinical GC samples. Finally, functional assays demonstrated the impact of FN1 knockdown on cellular proliferation, colony formation, and wound healing capacities.</p><p><strong>Conclusion: </strong>Overall, this study elucidates the crucial role of COL1A1, COL1A2, CHN1, and FN1 in GC pathogenesis and underscores their potential as diagnostic markers and therapeutic targets.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact and Significance of Viral Vectors for siRNA Delivery in the Treatment of Alzheimer's Disease.","authors":"Chintan Aundhia, Ghanshyam Parmar, Chitrali Talele, Rahul Trivedi, Mamta Kumari, Jay Chudasama","doi":"10.2174/0113892010334094241112190337","DOIUrl":"https://doi.org/10.2174/0113892010334094241112190337","url":null,"abstract":"<p><p>Alzheimer's disease (AD) remains a major challenge in developing effective treatments due to its complex pathophysiology, including the accumulation of amyloid-beta plaques and tau tangles. Small interfering RNA (siRNA) technology offers promise for targeted gene silencing, but effective delivery to the central nervous system remains a significant obstacle. Viral vectors have emerged as potent delivery vehicles for transporting siRNA to neural tissues. This review explores the utilization of viral vectors for siRNA delivery in AD, focusing on delivery strategies and challenges. We discuss the design and optimization of viral vectors, targeting strategies, and safety considerations. Additionally, we examine recent advancements and prospects for enhancing viral vector-mediated siRNA delivery in AD.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}