Comparative medicine最新文献_第8页

COVID-19 and Animal-Based Research. COVID-19和动物研究。

IF 0.8 4区农林科学

Comparative medicine Pub Date : 2021-10-01 DOI: 10.30802/AALAS-CM-21-000090

Jason S Villano

{"title":"COVID-19 and Animal-Based Research.","authors":"Jason S Villano","doi":"10.30802/AALAS-CM-21-000090","DOIUrl":"https://doi.org/10.30802/AALAS-CM-21-000090","url":null,"abstract":"In June 2017, AALAS published a special topic issue of Comparative Medicine on infectious disease research.14 The issue included articles on risk management, including occupational health and safety oversight5 and use of personal protective equipment,13 and finally, articles on 2 emerging diseases (Ebola9 and Zika1 virus infections) that were specifically chosen because, they were the most recent community outbreaks the world had seen. Fastforward to 2020, this special topic issue focuses on COVID-19, the new epitome of the modern-day pandemic. The pandemic started in late 2019 when cases of pneumonia of unknown etiology occurred in Wuhan, China. The novel virus was soon identified as severe acute respiratory syndromecoronavirus-2 (SARS-CoV-2), which caused a disease that has been termed as COVID-19, spreading throughout the world and officially being declared by the World Health Organization as a pandemic on March 11, 2020.12 As of end of September 2021, more than 230 million cases and 4.7 million deaths have occurred globally, with over 43 million cases and nearly 700,000 deaths in the United States.7 While we continue to navigate through this pandemic as it sickens people and claims lives, wreaking havoc to families, communities, and nations, we find science and technology center to global, national, and local response efforts to derail its destructive path.","PeriodicalId":10659,"journal":{"name":"Comparative medicine","volume":" ","pages":"331-332"},"PeriodicalIF":0.8,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594261/pdf/cm21000090.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39690653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hamsters as a Model of Severe Acute Respiratory Syndrome Coronavirus-2. 仓鼠作为严重急性呼吸综合征冠状病毒-2的模型

IF 0.8 4区农林科学

Comparative medicine Pub Date : 2021-10-01 Epub Date: 2021-09-29 DOI: 10.30802/AALAS-CM-21-000036

Alicia M Braxton, Patrick S Creisher, Camilo A Ruiz-Bedoya, Katie R Mulka, Santosh Dhakal, Alvaro A Ordonez, Sarah E Beck, Sanjay K Jain, Jason S Villano

{"title":"Hamsters as a Model of Severe Acute Respiratory Syndrome Coronavirus-2.","authors":"Alicia M Braxton, Patrick S Creisher, Camilo A Ruiz-Bedoya, Katie R Mulka, Santosh Dhakal, Alvaro A Ordonez, Sarah E Beck, Sanjay K Jain, Jason S Villano","doi":"10.30802/AALAS-CM-21-000036","DOIUrl":"10.30802/AALAS-CM-21-000036","url":null,"abstract":"Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of coronavirus disease 2019 (COVID-19), rapidly spread across the world in late 2019, leading to a pandemic. While SARS-CoV-2 infections predominately affect the respiratory system, severe infections can lead to renal and cardiac injury and even death. Due to its highly transmissible nature and severe health implications, animal models of SARS-CoV-2 are critical to developing novel therapeutics and preventatives. Syrian hamsters (Mesocricetus auratus) are an ideal animal model of SARS-CoV-2 infections because they recapitulate many aspects of human infections. After inoculation with SARS-CoV-2, hamsters become moribund, lose weight, and show varying degrees of respiratory disease, lethargy, and ruffled fur. Histopathologically, their pulmonary lesions are consistent with human infections including interstitial to broncho-interstitial pneumonia, alveolar hemorrhage and edema, and granulocyte infiltration. Similar to humans, the duration of clinical signs and pulmonary pathology are short lived with rapid recovery by 14 d after infection. Immunocompromised hamsters develop more severe infections and mortality. Preclinical studies in hamsters have shown efficacy of therapeutics, including convalescent serum treatment, and preventatives, including vaccination, in limiting or preventing clinical disease. Although hamster studies have contributed greatly to our understanding of the pathogenesis and progression of disease after SARS-CoV-2 infection, additional studies are required to better characterize the effects of age, sex, and virus variants on clinical outcomes in hamsters. This review aims to describe key findings from studies of hamsters infected with SARS-CoV-2 and to highlight areas that need further investigation.","PeriodicalId":10659,"journal":{"name":"Comparative medicine","volume":"71 5","pages":"398-410"},"PeriodicalIF":0.8,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594257/pdf/cm21000036.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9162608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

A Meta-Analysis of Rhesus Macaques (Macaca mulatta), Cynomolgus Macaques (Macaca fascicularis), African green monkeys (Chlorocebus aethiops), and Ferrets (Mustela putorius furo) as Large Animal Models for COVID-19. 恒河猴(Macaca mulatta)、食蟹猴(Macaca fascicularis)、非洲绿猴(Chlorocebus aethiops)和雪貂(Mustela putorius furo)作为COVID-19大型动物模型的meta分析

IF 0.8 4区农林科学

Comparative medicine Pub Date : 2021-10-01 Epub Date: 2021-09-29 DOI: 10.30802/AALAS-CM-21-000032

Alexandra N Witt, Rachel D Green, Andrew N Winterborn

{"title":"A Meta-Analysis of Rhesus Macaques (Macaca mulatta), Cynomolgus Macaques (Macaca fascicularis), African green monkeys (Chlorocebus aethiops), and Ferrets (Mustela putorius furo) as Large Animal Models for COVID-19.","authors":"Alexandra N Witt, Rachel D Green, Andrew N Winterborn","doi":"10.30802/AALAS-CM-21-000032","DOIUrl":"https://doi.org/10.30802/AALAS-CM-21-000032","url":null,"abstract":"Animal models are at the forefront of biomedical research for studies of viral transmission, vaccines, and pathogenesis, yet the need for an ideal large animal model for COVID-19 remains. We used a meta-analysis to evaluate published data relevant to this need. Our literature survey contained 22 studies with data relevant to the incidence of common COVID-19 symptoms in rhesus macaques (Macaca mulatta), cynomolgus macaques (Macaca fascicularis), African green monkeys (Chlorocebus aethiops), and ferrets (Mustela putorius furo). Rhesus macaques had leukocytosis on Day 1 after inoculation and pneumonia on Days 7 and 14 after inoculation, in frequencies that were similar enough to humans to reject the null hypothesis of a Fisher exact test. However, the differences in overall presentation of disease were too different from that of humans to successfully identify any of these 4 species as an ideal large animal of COVID-19. The greatest limitation to the current study is a lack of standardization in experimentation and reporting. To expand our understanding of the pathology of COVID-19 and evalu- ate vaccine immunogenicity, we must extend the unprecedented collaboration that has arisen in the study of COVID-19 to include standardization of animal-based research in an effort to find the optimal animal model.","PeriodicalId":10659,"journal":{"name":"Comparative medicine","volume":" ","pages":"433-441"},"PeriodicalIF":0.8,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594258/pdf/cm21000032.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39472051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The Effects of Ketamine on the Gut Microbiome on CD1 Mice. 氯胺酮对CD1小鼠肠道微生物组的影响。

IF 0.8 4区农林科学

Comparative medicine Pub Date : 2021-08-01 Epub Date: 2021-07-23 DOI: 10.30802/AALAS-CM-20-000117

Samantha A Gerb, Ryan J Dashek, Aaron C Ericsson, Rachel Griffin, Craig L Franklin

{"title":"The Effects of Ketamine on the Gut Microbiome on CD1 Mice.","authors":"Samantha A Gerb, Ryan J Dashek, Aaron C Ericsson, Rachel Griffin, Craig L Franklin","doi":"10.30802/AALAS-CM-20-000117","DOIUrl":"https://doi.org/10.30802/AALAS-CM-20-000117","url":null,"abstract":"The intestinal microbiota of an organism can significantly alter outcome data in otherwise identical experiments. Occasionally, animals may require sedation or anesthesia for scientific or health-related purposes, and certain anesthetics, such as ketamine, can profoundly affect the gastrointestinal system. While many factors can alter the gut microbiome (GM), the effects of anesthetics on the composition or diversity of the GM have not been established. The goal of the current study was to determine whether daily administration of ketamine would significantly alter the microbiome of CD1 mice. To achieve this goal, female CD1 mice received daily injections of ketamine HCl (100 mg/kg) or the equivalent volume of 0.9% saline for 10 consecutive days. Fecal samples were collected before the first administration and 24 h after the final dose of either ketamine or saline. Samples were analyzed by 16S rRNA sequencing to identify changes between groups in diversity or composition of GM. The study found no significant changes to the GM after serial ketamine administration when treated mice were housed with controls. Therefore, ketamine administration is unlikely to alter the GM of a CD1 mouse and should not serve be a confounding factor in reproducibility of research.","PeriodicalId":10659,"journal":{"name":"Comparative medicine","volume":"71 4","pages":"295-301"},"PeriodicalIF":0.8,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383998/pdf/cm2021000295.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39215898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Relationships between Slc1a5 and Osteoclastogenesis. Slc1a5与破骨细胞发生的关系。

IF 0.8 4区农林科学

Comparative medicine Pub Date : 2021-08-01 Epub Date: 2021-06-28 DOI: 10.30802/AALAS-CM-21-000012

Hideki Tsumura, Miyuki Shindo, Morihiro Ito, Arisa Igarashi, Kazue Takeda, Kenji Matsumoto, Takashi Ohkura, Kenji Miyado, Fumihiro Sugiyama, Akihiro Umezawa, Yasuhiko Ito

{"title":"Relationships between Slc1a5 and Osteoclastogenesis.","authors":"Hideki Tsumura, Miyuki Shindo, Morihiro Ito, Arisa Igarashi, Kazue Takeda, Kenji Matsumoto, Takashi Ohkura, Kenji Miyado, Fumihiro Sugiyama, Akihiro Umezawa, Yasuhiko Ito","doi":"10.30802/AALAS-CM-21-000012","DOIUrl":"https://doi.org/10.30802/AALAS-CM-21-000012","url":null,"abstract":"Slc1a5 (ASCT2) encodes a small neutral amino-acid exchanger and is the most well-studied glutamine transporter in cancer cells. To investigate the role of Slc1a5 in osteoclastogenesis, we developed Slc1a5-deficient mice by using a conventional gene-targeting approach. The Slc1a5-/- mice showed no obvious abnormalities in growth. Glutamine uptake was assessed in Slc1a5+/+ and Slc1a5-/- bone marrow cells stimulated with RANKL. The rate of glutamine uptake in Slc1a5-/- bone marrow cells was reduced to 70% of that of cells from Slc1a5+/+ bone marrow. To confirm the involvement of Slc1a5 in osteoclast formation, bone marrow cells derived from Slc1a5+/+ or Slc1a5-/- mice were stimulated with RANKL and macrophage colony-stimulating factor and stained with tartrate-resistant acid phosphatase. The bone resorption activity and actin ring formation of stimulated cells were measured. The formation of multinucleated osteoclasts in bone marrow cells isolated from Slc1a5-/- mice was severely impaired compared with those from Slc1a5+/+ mice. RANKL-induced expression of ERK, NFκB, p70S6K, and NFATc1 was suppressed in Slc1a5-/- osteoclasts. These results show that Slc1a5 plays an important role in osteoclast formation.","PeriodicalId":10659,"journal":{"name":"Comparative medicine","volume":"71 4","pages":"285-294"},"PeriodicalIF":0.8,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384000/pdf/cm2021000285.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39215897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Edwardsiella ictaluri in a Colony of Zebrafish (Danio rerio) Used in a Teaching Laboratory. 斑马鱼群体中的爱德华氏菌(斑马鱼版)在教学实验室中的应用。

IF 0.8 4区农林科学

Comparative medicine Pub Date : 2021-08-01 Epub Date: 2021-06-29 DOI: 10.30802/AALAS-CM-21-000034

Francis J Sun, Marcus J Crim, Mathias Leblanc

{"title":"Edwardsiella ictaluri in a Colony of Zebrafish (Danio rerio) Used in a Teaching Laboratory.","authors":"Francis J Sun, Marcus J Crim, Mathias Leblanc","doi":"10.30802/AALAS-CM-21-000034","DOIUrl":"https://doi.org/10.30802/AALAS-CM-21-000034","url":null,"abstract":"A small colony of zebrafish (Danio rerio) experienced 30% acute mortality within a few days after receipt from a commercial source. A few fish presented with small areas of raised scales or tissue necrosis, primarily near the caudal peduncle. Edwardsiella ictaluri (E. ictaluri) was identified by real-time PCR of pooled zebrafish and swabs of the pre-filter and fine filter pads, with subsequent sequence analysis. E. ictaluri is most commonly associated with an enteric septicemia in catfish species and can have significant economic impact on commercial catfish fisheries. However, several references report naturally occurring E. ictaluri infection of nonictalurid fishes, including zebrafish. Ours is the first report demonstrating the use of environmental sampling to identify E. ictaluri in a zebrafish colony by real-time PCR. Moreover, our report indicates that E. ictaluri is a relevant disease for institutions using zebrafish as research species and emphasizes the importance of carefully considering importation and quarantine practices.","PeriodicalId":10659,"journal":{"name":"Comparative medicine","volume":"71 4","pages":"318-322"},"PeriodicalIF":0.8,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8384001/pdf/cm2021000318.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39039655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Review of Principal Studies on the Development and Treatment of Epithelial Ovarian Cancer in the Laying Hen Gallus gallus. 蛋鸡上皮性卵巢癌发生及治疗的主要研究综述。

IF 0.8 4区农林科学

Comparative medicine Pub Date : 2021-08-01 Epub Date: 2021-07-29 DOI: 10.30802/AALAS-CM-20-000116

Purab Pal, Kara Nicole Starkweather, Karen Held Hales, Dale Buchanan Hales

{"title":"A Review of Principal Studies on the Development and Treatment of Epithelial Ovarian Cancer in the Laying Hen Gallus gallus.","authors":"Purab Pal, Kara Nicole Starkweather, Karen Held Hales, Dale Buchanan Hales","doi":"10.30802/AALAS-CM-20-000116","DOIUrl":"https://doi.org/10.30802/AALAS-CM-20-000116","url":null,"abstract":"Often referred to as the silent killer, ovarian cancer is the most lethal gynecologic malignancy. This disease rarely shows any physical symptoms until late stages and no known biomarkers are available for early detection. Because ovarian cancer is rarely detected early, the physiology behind the initiation, progression, treatment, and prevention of this disease remains largely unclear. Over the past 2 decades, the laying hen has emerged as a model that naturally develops epithelial ovarian cancer that is both pathologically and histologically similar to that of the human form of the disease. Different molecular signatures found in human ovarian cancer have also been identified in chicken ovarian cancer including increased CA125 and elevated E-cadherin expression, among others. Chemoprevention studies conducted in this model have shown that decreased ovulation and inflammation are associated with decreased incidence of ovarian cancer development. The purpose of this article is to review the major studies performed in laying hen model of ovarian cancer and discuss how these studies shape our current understanding of the pathophysiology, prevention, and treatment of epithelial ovarian cancer.","PeriodicalId":10659,"journal":{"name":"Comparative medicine","volume":"71 4","pages":"271-284"},"PeriodicalIF":0.8,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383999/pdf/cm2021000271.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39257153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Psychometrics of the Balance Beam Functional Test in C57BL/6 Mice. C57BL/6小鼠平衡木功能测试的心理测量学。

IF 0.8 4区农林科学

Comparative medicine Pub Date : 2021-08-01 Epub Date: 2021-07-26 DOI: 10.30802/AALAS-CM-21-000033

Melissa C Orenduff, Erika T Rezeli, Stephen D Hursting, Carl F Pieper

{"title":"Psychometrics of the Balance Beam Functional Test in C57BL/6 Mice.","authors":"Melissa C Orenduff, Erika T Rezeli, Stephen D Hursting, Carl F Pieper","doi":"10.30802/AALAS-CM-21-000033","DOIUrl":"https://doi.org/10.30802/AALAS-CM-21-000033","url":null,"abstract":"Aging is associated with a progressive decline in physical function characterized by decreased mobility, which is an important risk factor for loss of independence and reduced quality of life. Functional testing conducted in animals has advanced our understanding of age-related changes in physical ability and contributed to the development of physiologic measurements that can be used to assess functional changes during aging. The balance beam test is one assessment tool used to measure age-related changes in balance and coordination. The goal of this study is to provide analytical examples and psychometric support of a protocol that has been analyzed to show how the number of successive test runs, foot slips, pauses, and hesitations affect the reliability of the primary outcome measure, which is the time to cross the beam. Our results suggest that conducting more than 1 training session, consisting of greater than or equal to 3 successful training runs, followed by at least one test session with no less than 2 successful runs (that is, runs without pauses or hesitations) provides a psychometrically sound outcome. The data presented here indicate that a psychometric approach can improve protocol design and reliability of balance beam measures in mice.","PeriodicalId":10659,"journal":{"name":"Comparative medicine","volume":"71 4","pages":"302-308"},"PeriodicalIF":0.8,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383997/pdf/cm2021000302.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39224903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Dietary Melatonin and Omega-3 Fatty Acids Induce Human Cancer Xenograft Regression In Vivo in Rats by Suppressing Linoleic Acid Uptake and Metabolism. 膳食褪黑素和Omega-3脂肪酸通过抑制亚油酸的摄取和代谢，诱导体内大鼠癌症异种移植物消退。

IF 0.8 4区农林科学

Comparative medicine Pub Date : 2021-08-01 Epub Date: 2021-06-29 DOI: 10.30802/AALAS-CM-21-000025

Erin M Dauchy, Robert T Dauchy, Robert P Tirrell, Leslie K Davidson, Steven M Hill, Leonard A Sauer, David E Blask

{"title":"Dietary Melatonin and Omega-3 Fatty Acids Induce Human Cancer Xenograft Regression In Vivo in Rats by Suppressing Linoleic Acid Uptake and Metabolism.","authors":"Erin M Dauchy, Robert T Dauchy, Robert P Tirrell, Leslie K Davidson, Steven M Hill, Leonard A Sauer, David E Blask","doi":"10.30802/AALAS-CM-21-000025","DOIUrl":"https://doi.org/10.30802/AALAS-CM-21-000025","url":null,"abstract":"Melatonin, the circadian nighttime neurohormone, and eicosapentaenoic acid (EPA) and docosahexaenoic acids (DHA), which are omega-3 fatty acids (FA) found in high concentrations in fish oil (FO) and plants, abrogate the oncogenic effects of linoleic acid (LA), an omega-6 FA, on the growth of rodent tumors and human breast, prostate, and head and neck squamous cell carcinoma (HNSCC) xenografts in vivo. Here we determined and compared the long-term effects of these inhibitory agents on tumor regression and LA uptake and metabolism to the mitogenic agent 13-[S]-hydroxyoctadecadienoic acid (13-[S]-HODE) in human prostate cancer 3 (PC3) and FaDu HNSCC xenografts in tumor-bearing male nude rats. Rats in this study were split into 3 groups and fed one of 2 diets: one diet containing 5% corn oil (CO, high LA), 5% CO oil and melatonin (2 μg/mL) or an alternative diet 5% FO (low LA). Rats whose diet contained melatonin had a faster rate of regression of PC3 prostate cancer xenografts than those receiving the FO diet, while both in the melatonin and FO groups induced the same rate of regression of HNSCC xenografts. The results also demonstrated that dietary intake of melatonin or FO significantly inhibited tumor LA uptake, cAMP content, 13-[S]-HODE formation, [³H]-thymidine incorporation into tumor DNA, and tumor DNA content. Therefore, long-term ingestion of either melatonin or FO can induce regression of PC3 prostate and HNSCC xenografts via a mechanism involving the suppression of LA uptake and metabolism by the tumor cells.","PeriodicalId":10659,"journal":{"name":"Comparative medicine","volume":"71 4","pages":"309-317"},"PeriodicalIF":0.8,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383996/pdf/cm2021000309.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39118444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Strategies to Improve Survival from Surgery for Heart Valve Implantation in Sheep. 提高绵羊心脏瓣膜植入术后存活率的策略。

IF 0.8 4区农林科学

Comparative medicine Pub Date : 2021-06-01 Epub Date: 2021-06-03 DOI: 10.30802/AALAS-CM-20-000118

Annemijn Vis, Jan Cam Lammers, Roel de Vroege, Martijn Mj van Nieuwburg, Marlijn S Jansen, Joyce Mj Visser, Bart Meuris, Paul F Gründeman, Jolanda Kluin

{"title":"Strategies to Improve Survival from Surgery for Heart Valve Implantation in Sheep.","authors":"Annemijn Vis, Jan Cam Lammers, Roel de Vroege, Martijn Mj van Nieuwburg, Marlijn S Jansen, Joyce Mj Visser, Bart Meuris, Paul F Gründeman, Jolanda Kluin","doi":"10.30802/AALAS-CM-20-000118","DOIUrl":"https://doi.org/10.30802/AALAS-CM-20-000118","url":null,"abstract":"Sheep are a commonly used and validated model for cardiovascular research and, more specifically, for heart valve research. Implanting a heart valve on the arrested heart in sheep is complex and is often complicated by difficulties in restarting the heart, causing significant on-table mortality. Therefore, optimal cardioprotective management during heart valve implantation in sheep is essential. However, little is known about successful cardioprotective management techniques in sheep. This article reports our experience in the cardioprotective management of 20 female sheep that underwent surgical aortic valve replacement with a stented tissue-engineered heart valve prosthesis. During this series of experiments, we modified our cardioprotection protocol to improve survival. We emphasize the importance of total body hypothermia and external cooling of the heart. Furthermore, we recommend repeated cardioplegia administration at 20 min intervals during surgery, with the final dosage of cardioplegia given immediately before the de-clamping of the aorta. To reduce the number of defibrillator shocks during a state of ventricular fibrillation (VF), we have learned to restart the heart by reclamping the aorta, administering cardioplegia until cardiac arrest, and de-clamping the aorta thereafter. Despite these encouraging results, more research is needed to finalize a protocol for this procedure.","PeriodicalId":10659,"journal":{"name":"Comparative medicine","volume":"71 3","pages":"235-239"},"PeriodicalIF":0.8,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8223872/pdf/cm2021000235.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38978316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0