Communications Chemistry最新文献

Metastable phase-separated droplet generation and long-time DNA enrichment by laser-induced Soret effect. 利用激光诱导的索雷特效应生成可转移的相分离液滴并长时间富集 DNA。

IF 5.9 2区化学

Communications Chemistry Pub Date : 2025-02-28 DOI: 10.1038/s42004-025-01438-w

Mika Kobayashi, Yoshihiro Minagawa, Hiroyuki Noji

{"title":"Metastable phase-separated droplet generation and long-time DNA enrichment by laser-induced Soret effect.","authors":"Mika Kobayashi, Yoshihiro Minagawa, Hiroyuki Noji","doi":"10.1038/s42004-025-01438-w","DOIUrl":"https://doi.org/10.1038/s42004-025-01438-w","url":null,"abstract":"Spatiotemporally controlled laser-induced phase separation (LIPS) offers unique research avenues and has potential for biological and biomedical applications. However, LIPS conditions often have drawbacks for practical use, which limit their applications. For instance, LIPS droplets are unstable and diminish after the laser is terminated. Here, we developed a novel LIPS method using laser-induced Soret effect with a simple setup to solve these problems. We generate liquid-liquid phase-separated (LLPS) droplets using LIPS in an aqueous two-phase system (ATPS) of dextran (DEX) and polyethylene glycol (PEG). When DEX-rich droplets were generated in the DEX/PEG mix on the phase boundary, the droplets showed unprecedently high longevity; the DEX droplets were retained over 48 h. This counterintuitive behaviour suggests that the droplet is in an unknown metastable state. By exploiting the capability of DEX-rich droplets to enrich nucleic acid polymers, we achieved stable DNA enrichment in LIPS DEX droplets with a high enrichment factor of 1400 ± 400. Further, we patterned DNA-carrying DEX-rich droplets into a designed structure to demonstrate the stability and spatiotemporal controllability of DEX-rich droplet formation. This is the first report for LIPS droplet generation in a DEX/PEG system, opening new avenues for biological and medical applications of LIPS.","PeriodicalId":10529,"journal":{"name":"Communications Chemistry","volume":"8 1","pages":"61"},"PeriodicalIF":5.9,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Author Correction: Proteome-wide non-cleavable crosslink identification with MS Annika 3.0 reveals the structure of the C. elegans Box C/D complex.

IF 5.9 2区化学

Communications Chemistry Pub Date : 2025-02-27 DOI: 10.1038/s42004-025-01461-x

Micha J Birklbauer, Fränze Müller, Sowmya Sivakumar Geetha, Manuel Matzinger, Karl Mechtler, Viktoria Dorfer

引用次数: 0

Revealing the molecular interplay of coverage, wettability, and capacitive response at the Pt(111)-water solution interface under bias.

IF 5.9 2区化学

Communications Chemistry Pub Date : 2025-02-24 DOI: 10.1038/s42004-025-01446-w

Federico Raffone, Rémi Khatib, Marialore Sulpizi, Clotilde Cucinotta

{"title":"Revealing the molecular interplay of coverage, wettability, and capacitive response at the Pt(111)-water solution interface under bias.","authors":"Federico Raffone, Rémi Khatib, Marialore Sulpizi, Clotilde Cucinotta","doi":"10.1038/s42004-025-01446-w","DOIUrl":"10.1038/s42004-025-01446-w","url":null,"abstract":"While electrified interfaces are crucial for electrocatalysis and corrosion, their molecular morphology remains largely unknown. Through highly realistic ab initio molecular dynamics simulations of the Pt(111)-water solution interface in reducing conditions, we reveal a deep interconnection among electrode coverage, wettability, capacitive response, and catalytic activity. We identify computationally the experimentally hypothesised states for adsorbed hydrogen on Pt, HUPD and HOPD, revealing their role in governing interfacial water reorientation and hydrogen evolution. The transition between these two H states with increasing potential, induces a shift from a hydrophobic to a hydrophilic interface and correlates with a change in the primary electrode screening mechanism. This results in a slope change in differential capacitance, marking the onset of the experimentally observed peak around the potential of zero charge. Our work produces crucial insights for advancing electrocatalytic energy conversion, developing deep understanding of electrified interfaces.","PeriodicalId":10529,"journal":{"name":"Communications Chemistry","volume":"8 1","pages":"58"},"PeriodicalIF":5.9,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850831/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Author Correction: Structural phase transition in NH₄F under extreme pressure conditions.

IF 5.9 2区化学

Communications Chemistry Pub Date : 2025-02-24 DOI: 10.1038/s42004-025-01457-7

Umbertoluca Ranieri, Christophe Bellin, Lewis J Conway, Richard Gaal, John S Loveday, Andreas Hermann, Abhay Shukla, Livia E Bove

引用次数: 0

Nanomolar inhibitor of the galectin-8 N-terminal domain binds via a non-canonical cation-π interaction. galectin-8 N 端结构域的纳摩尔抑制剂通过非典型阳离子-π相互作用结合。

IF 5.9 2区化学

Communications Chemistry Pub Date : 2025-02-24 DOI: 10.1038/s42004-025-01458-6

Edvin Purić, Mujtaba Hassan, Fredrik Sjövall, Tihomir Tomašič, Mojca Pevec, Jurij Lah, Jaume Adrover Forteza, Anders Sundin, Hakon Leffler, Ulf J Nilsson, Derek T Logan, Marko Anderluh

{"title":"Nanomolar inhibitor of the galectin-8 N-terminal domain binds via a non-canonical cation-π interaction.","authors":"Edvin Purić, Mujtaba Hassan, Fredrik Sjövall, Tihomir Tomašič, Mojca Pevec, Jurij Lah, Jaume Adrover Forteza, Anders Sundin, Hakon Leffler, Ulf J Nilsson, Derek T Logan, Marko Anderluh","doi":"10.1038/s42004-025-01458-6","DOIUrl":"10.1038/s42004-025-01458-6","url":null,"abstract":"Galectin-8 is a tandem-repeat galectin consisting of two distinct carbohydrate recognition domains and is a potential drug target. We have developed a library of galectin-8N inhibitors that exhibit high nanomolar Kd values as determined by a competitive fluorescence polarization assay. A detailed thermodynamic analysis of the binding of D-galactosides to galectin-8N by isothermal titration calorimetry reveals important differences in enthalpic and/or entropic contributions to binding. Contrary to expectations, the binding of 2-O-propargyl-D-galactoside was found to strongly increase the binding enthalpy, whereas the binding of 2-O-carboxymethylene-D-galactoside was surprisingly less enthalpy-driven. The results of our work suggest that the ethynyl group can successfully replace the carboxylate group when targeting the water-exposed guanidine moiety of a critical arginine residue. This results in only a minor loss of affinity and an adjusted enthalpic contribution to the overall binding due to non-canonical cation-π interactions, as evidenced by the obtained crystal structure of 2-O-propargyl-D-galactoside in complex with the N-terminal domain of galectin-8. Such an interaction has neither been identified nor discussed to date in a small-molecule ligand-protein complex.","PeriodicalId":10529,"journal":{"name":"Communications Chemistry","volume":"8 1","pages":"59"},"PeriodicalIF":5.9,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of electrochemical hydrogenation of aromatic compound mixtures over a bimetallic PtRu catalyst.

IF 5.9 2区化学

Communications Chemistry Pub Date : 2025-02-23 DOI: 10.1038/s42004-025-01413-5

Cesar Catizane, Ying Jiang, Joy Sumner

引用次数: 0

Women in chemistry: Q&A with Professor Carolina Horta Andrade.

IF 5.9 2区化学

Communications Chemistry Pub Date : 2025-02-22 DOI: 10.1038/s42004-025-01452-y

引用次数: 0

Women in chemistry: Q&A with Dr Laurie Barge.

IF 5.9 2区化学

Communications Chemistry Pub Date : 2025-02-22 DOI: 10.1038/s42004-025-01442-0

引用次数: 0

Rapid peptide synthesis using a methylimidazolium sulfinyl fluoride salt.

IF 5.9 2区化学

Communications Chemistry Pub Date : 2025-02-22 DOI: 10.1038/s42004-025-01456-8

Joey Lai, Carlota Bahri, Mai P Truong, Kathleen T Downey, Glenn M Sammis

{"title":"Rapid peptide synthesis using a methylimidazolium sulfinyl fluoride salt.","authors":"Joey Lai, Carlota Bahri, Mai P Truong, Kathleen T Downey, Glenn M Sammis","doi":"10.1038/s42004-025-01456-8","DOIUrl":"10.1038/s42004-025-01456-8","url":null,"abstract":"Peptide couplings have been a subject of investigation for over a century, with modern research seeking to discover new methodologies that minimize purification steps, minimize reagent expense, and/or decrease reaction times. Of the numerous coupling reagents available, sulfur(IV) fluorides have potential as they can effectively transform carboxylic acids to reactive intermediates, and the sulfite by-products can be removed through aqueous washes. Here we demonstrate the formation and capture of key acyl fluorosulfite intermediates for peptide couplings in 15 min total, without epimerization or column chromatography for purification. Dipeptides were obtained in 40-94% yields. This approach was expanded to longer chains through iterative couplings, with oligopeptides obtained in 24-57% yields, each within 2 days. Mechanistic studies indicate the reaction does not proceed through acyl fluoride intermediates, and instead involves nucleophilic catalysis. The mild conditions are tolerant of a wide range of protecting groups of canonical and non-canonical amino acids.","PeriodicalId":10529,"journal":{"name":"Communications Chemistry","volume":"8 1","pages":"53"},"PeriodicalIF":5.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11846836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Catalytic dwell oscillations complete the F₁-ATPase mechanism.

IF 5.9 2区化学

Communications Chemistry Pub Date : 2025-02-21 DOI: 10.1038/s42004-025-01443-z

Zain A Bukhari, Wayne D Frasch

{"title":"Catalytic dwell oscillations complete the F1-ATPase mechanism.","authors":"Zain A Bukhari, Wayne D Frasch","doi":"10.1038/s42004-025-01443-z","DOIUrl":"10.1038/s42004-025-01443-z","url":null,"abstract":"The F1-ATPase molecular motor rotates subunit-γ in 120° power strokes within its ring of three catalytic sites separated by catalytic dwells for ATP hydrolysis and Pi release. By monitoring rotary position of subunit-γ in E. coli F1 every 5 μs, we resolved Stage-1 catalytic dwell oscillations that extend from -13° to 13° centered at 0° consistent with F1 structures containing transition state inhibitors, which decay by a first order process consistent with ATP hydrolysis. During Stage-2, 80% of the oscillations extend from 3° and 25° centered at 14°, while 20% are centered at 33° and can extend to 27°-44° comparable to the ATP binding position. Remarkably, in Stage-3 subunit-γ returns to 0° to end the catalytic dwell, which keeps the start of power strokes in phase for consecutive rotational events. These newly observed states fit with F1 structures that were inconsistent with the canonical mechanism, and indicate that catalytic dwell oscillations must persist until the correct occupancy of substrates and products occurs at all three catalytic sites. When that condition is met, F1 can proceed to the next power stroke. Understanding the basis of these catalytic dwell oscillations completes the F1-ATPase rotary mechanism.","PeriodicalId":10529,"journal":{"name":"Communications Chemistry","volume":"8 1","pages":"52"},"PeriodicalIF":5.9,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11845608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0