Clinica Chimica Acta最新文献_第2页

Long cardiac troponin T forms in a healthy reference population 健康参考人群的长心肌肌钙蛋白T形成

IF 3.2 3区医学

Clinica Chimica Acta Pub Date : 2025-06-07 DOI: 10.1016/j.cca.2025.120419

Tuulia Tuominen , Tuija Vasankari , Helea Junes , Selma Salonen , Konsta Teppo , Anna Linko-Parvinen , Hanna-Mari Pallari , K.E. Juhani Airaksinen , Saara Wittfooth

{"title":"Long cardiac troponin T forms in a healthy reference population","authors":"Tuulia Tuominen , Tuija Vasankari , Helea Junes , Selma Salonen , Konsta Teppo , Anna Linko-Parvinen , Hanna-Mari Pallari , K.E. Juhani Airaksinen , Saara Wittfooth","doi":"10.1016/j.cca.2025.120419","DOIUrl":"10.1016/j.cca.2025.120419","url":null,"abstract":"<div><h3>Background</h3><div>Cardiac troponin T (cTnT) is an established biomarker in the diagnosis of myocardial infarction (MI). Recent studies show better discrimination between MI and other conditions when measuring only intact or minimally fragmented cTnT forms (long cTnT), compared to current cTnT assays that measure both intact and highly fragmented cTnT forms (total cTnT). This study investigated the long cTnT concentrations in a healthy population.</div></div><div><h3>Methods</h3><div>Lithium-heparin plasma samples were collected from 314 healthy volunteers aged 21–85 years (59 % female). The samples were analyzed for total cTnT with Roche Elecsys high sensitivity cTnT assay and with a novel upconversion luminescence-based long cTnT assay.</div></div><div><h3>Results</h3><div>The median (25th-75th percentile) long cTnT concentration of the reference population was 2.0 (1.3–3.0) ng/l. The 99th percentile URL for the long cTnT assay was 7.3 (95 % confidence interval, 5.6–8.4) ng/l. Of the healthy population 98 % had long cTnT levels above the detection limit of the assay (0.4 ng/l). The imprecision (coefficient of variation) of the long cTnT assay at the 99th percentile URL was 5.1 %. While total cTnT was heavily associated with age, especially in the older population, long cTnT remained low regardless of age. Common comorbidities and medications were also associated with the total cTnT concentration but not with the long cTnT concentration.</div></div><div><h3>Conclusions</h3><div>The long cTnT assay fulfills the requirements for a high sensitivity cTnT assay. Healthy individuals have low long cTnT concentrations regardless of age and common comorbidities.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"576 ","pages":"Article 120419"},"PeriodicalIF":3.2,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-coding RNAs in celiac disease 乳糜泻中的非编码rna

IF 3.2 3区医学

Clinica Chimica Acta Pub Date : 2025-06-06 DOI: 10.1016/j.cca.2025.120417

Asma Vafadar , Elham Shirazi-Tehrani , Parisa Vosough , Shayan Khalili Alashti , Hossein Kargar Jahromi , Kamran Bagheri Lankarani , Amir Savardashtaki , Sajad Ehtiati

引用次数: 0

Methodological limitations in the diagnostic validation of P-C4BP-Rf for ovarian clear cell carcinoma versus endometrioma: addressing sample size constraints and selective bias P-C4BP-Rf诊断卵巢透明细胞癌与子宫内膜瘤的方法学局限性：解决样本量限制和选择性偏倚

IF 3.2 3区医学

Clinica Chimica Acta Pub Date : 2025-06-04 DOI: 10.1016/j.cca.2025.120416

Meibo Chen, Shanshan Yuan

引用次数: 0

Establishment of a microsphere-based flow cytometric assay for quantitative detection of total immunoglobulin E 建立微球流式细胞术定量检测总免疫球蛋白E的方法

IF 3.2 3区医学

Clinica Chimica Acta Pub Date : 2025-06-04 DOI: 10.1016/j.cca.2025.120415

Qi Jiang , Yuanmin Sun , Xiaoming Cui , Yixian Li , Xiaohui Yang , Xue Li , Huiqiang Li , Yang Yu

{"title":"Establishment of a microsphere-based flow cytometric assay for quantitative detection of total immunoglobulin E","authors":"Qi Jiang , Yuanmin Sun , Xiaoming Cui , Yixian Li , Xiaohui Yang , Xue Li , Huiqiang Li , Yang Yu","doi":"10.1016/j.cca.2025.120415","DOIUrl":"10.1016/j.cca.2025.120415","url":null,"abstract":"<div><h3>Background</h3><div>In the diagnosis of allergic diseases, in vitro serum IgE level tests were conventionally used. In recent years, microsphere-based flow cytometric assays for IgE have gained more attention. The present study aimed to establish a microsphere-based flow cytometric assay for quantitative detection of tIgE.</div></div><div><h3>Methods</h3><div>The assay was established after selection of microsphere, antibody pair, detection mode, and optimization of coating ratio. In order to quantitate tIgE, the calibration curve was established. The detection performance of this new tIgE flow cytometric assay was also confirmed. In addition, the correlations between this assay with the ImmunoCAP assay or the immunoturbidimetry assay were evaluated.</div></div><div><h3>Results</h3><div>This new tIgE flow cytometric assay has low CVs of repeatability (7.62 % and 6.98 %) and intermediate precision (7.50 % and 8.41 %). The detection range was 2 ∼ 6000 IU/mL. The range of linearity was from 2 ∼ 3363 IU/L (<em>r</em> = 0.9932). The correlation coefficient (r) for the correlation analysis between the results of the newly developed tIgE flow cytometric assay and ImmunoCAP assay or immunoturbidimetry assay was 0.9345 and 0.9348, respectively.</div></div><div><h3>Conclusion</h3><div>A tIgE flow cytometric assay was successfully established. It could be used in clinical laboratories and quantitate lots of allergen-sIgE detected by flow cytometric assay.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"576 ","pages":"Article 120415"},"PeriodicalIF":3.2,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144241486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BDNF biosensors for neurodegenerative disease 神经退行性疾病的BDNF生物传感器

IF 3.2 3区医学

Clinica Chimica Acta Pub Date : 2025-06-03 DOI: 10.1016/j.cca.2025.120412

Tamara Nazar Saeed , Ali Fawzi Al-Hussainy , Gaurav Sanghvi , Suhas Ballal , Abhayveer Singh , A. Sabarivani , Swati Mishra , Jasur Rizaev , Sada Ghalib Taher , Mariem Alwan , Mahmood Jawad , Hiba Mushtaq

{"title":"BDNF biosensors for neurodegenerative disease","authors":"Tamara Nazar Saeed , Ali Fawzi Al-Hussainy , Gaurav Sanghvi , Suhas Ballal , Abhayveer Singh , A. Sabarivani , Swati Mishra , Jasur Rizaev , Sada Ghalib Taher , Mariem Alwan , Mahmood Jawad , Hiba Mushtaq","doi":"10.1016/j.cca.2025.120412","DOIUrl":"10.1016/j.cca.2025.120412","url":null,"abstract":"<div><div>The brain-derived neurotrophic factor (BDNF) is essential for neuronal health and function. Dysregulated BDNF levels have been correlated with the etiology of several neurological disorders, including Alzheimer’s and Parkinson’s diseases. Recently, biomarkers of central nervous system (CNS) inflammatory responses have been discovered in a variety of body fluids, including blood, cerebrospinal fluid (CSF), and tears. Personalized medicine and screening programs will become feasible once biosensing technologies advance sufficiently and are widely implemented in communities. This multidisciplinary review provides an overview of the molecular structure and synthesis of BDNF, emphasizing the complexity and intricate regulation of this crucial neurotrophic factor, as well as exploring the potential of BDNF as a biomarker for neurological conditions. It focuses on its role in disease progression and its utility in early disease detection using aptasensors and immunosensors. We also discuss the challenges associated with utilizing BDNF as a biomarker, such as its complex regulation and the need for sensitive and specific detection methods by electrochemical and optical transducers. Furthermore, we highlight the potential of biosensors to overcome these challenges by enabling real-time, non-invasive, and point-of-care (POC) monitoring of BDNF levels.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"576 ","pages":"Article 120412"},"PeriodicalIF":3.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144229684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanosensing for tramadol detection: Innovative opioid analysis 曲马多检测的纳米传感：创新的阿片分析

IF 3.2 3区医学

Clinica Chimica Acta Pub Date : 2025-06-02 DOI: 10.1016/j.cca.2025.120414

Ilghar Zeinaly , Ali Ahmadalipour , Mohammad Mahdi Heidari , Shahab Masoumi , Arash Mohagheghi , Ahmad Mobed

{"title":"Nanosensing for tramadol detection: Innovative opioid analysis","authors":"Ilghar Zeinaly , Ali Ahmadalipour , Mohammad Mahdi Heidari , Shahab Masoumi , Arash Mohagheghi , Ahmad Mobed","doi":"10.1016/j.cca.2025.120414","DOIUrl":"10.1016/j.cca.2025.120414","url":null,"abstract":"<div><div>Tramadol, a widely used analgesic, plays a significant role in pain management but poses risks of misuse and addiction, necessitating accurate detection methods. Traditional analytical techniques for opioid determination, such as chromatography and mass spectrometry, often face limitations, including lengthy processing times, high costs, and the need for specialized equipment. In contrast, nanosensing technologies have emerged as innovative approaches for the sensitive and rapid detection of tramadol at the nanoscale. Over the past decade, extensive advancements in nanosensor development have demonstrated their potential to overcome the limitations of conventional methods. This paper aims to introduce and discuss recently developed nanosensors specifically designed for tramadol detection, highlighting their significance in pharmacology, forensic medicine, and point-of-care (POC) applications. By providing enhanced sensitivity, specificity, and real-time analysis, these nanosensing techniques signify a groundbreaking advancement in the efficient monitoring and management of tramadol usage.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"576 ","pages":"Article 120414"},"PeriodicalIF":3.2,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomarkers in liver regeneration 肝脏再生中的生物标志物。

IF 3.2 3区医学

Clinica Chimica Acta Pub Date : 2025-06-01 DOI: 10.1016/j.cca.2025.120413

Radjabov Akhtam , Shavazi Nargiz Nuraliyevna , Mustafa Jawad Kadham , Kosimova Shairakhon Mirzakhamitovna , Rasulova Mokhidil Tursunaliyevna , Khujaeva Shakhnoz , Turabekov Shakhzod , Bobojonov Otabek , Matkarimov Inomjon Baxtiyorovich , Akhmedova Feruzakhon Shakhboskhanovna , Boymatova Zulxumorxon , Ismoilova Muazzamxon Isroilovna , Nilufar Rakhmonkulova Khodji-Akbarovna

{"title":"Biomarkers in liver regeneration","authors":"Radjabov Akhtam , Shavazi Nargiz Nuraliyevna , Mustafa Jawad Kadham , Kosimova Shairakhon Mirzakhamitovna , Rasulova Mokhidil Tursunaliyevna , Khujaeva Shakhnoz , Turabekov Shakhzod , Bobojonov Otabek , Matkarimov Inomjon Baxtiyorovich , Akhmedova Feruzakhon Shakhboskhanovna , Boymatova Zulxumorxon , Ismoilova Muazzamxon Isroilovna , Nilufar Rakhmonkulova Khodji-Akbarovna","doi":"10.1016/j.cca.2025.120413","DOIUrl":"10.1016/j.cca.2025.120413","url":null,"abstract":"<div><div>Liver regeneration is a complex and tightly regulated process essential for maintaining organ function following injury or surgical resection. This work explores the molecular mechanisms and key biomarkers involved in liver regeneration, highlighting their role in monitoring, predicting, and enhancing hepatic recovery. Conventional liver function tests, such as ALT and AST, provide insights into liver injury but lack specificity regarding regenerative capacity. Emerging biomarkers, including miRNAs, nuclear receptors, and native peptides, offer a more dynamic assessment of liver regeneration. Additionally, metabolomic and transcriptomic analyses revealed crucial pathways that drive hepatocyte proliferation and tissue repair. Understanding these biomarkers and regulatory networks is vital for improving clinical outcomes, preventing complications such as posthepatectomy liver failure, and advancing therapeutic strategies for liver diseases.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"576 ","pages":"Article 120413"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-coding RNA biomarkers in basal-like breast cancer 基底样乳腺癌中的非编码RNA生物标志物

IF 3.2 3区医学

Clinica Chimica Acta Pub Date : 2025-05-31 DOI: 10.1016/j.cca.2025.120408

Haider Ali , Nawaid Hussain Khan , Dalila Cano , Guadalupe Cano , Meliha Celik , Sama Lilo , Angel Fabia , Yama A. , Efrain Gudino , Beyza Nur Beker

{"title":"Non-coding RNA biomarkers in basal-like breast cancer","authors":"Haider Ali , Nawaid Hussain Khan , Dalila Cano , Guadalupe Cano , Meliha Celik , Sama Lilo , Angel Fabia , Yama A. , Efrain Gudino , Beyza Nur Beker","doi":"10.1016/j.cca.2025.120408","DOIUrl":"10.1016/j.cca.2025.120408","url":null,"abstract":"<div><div>Basal-like breast cancer (BLBC) is a highly aggressive molecular subtype characterized by poor prognosis and limited targeted treatment options. Clinicians face challenges in early diagnosis and precise prognostic stratification, necessitating the discovery of novel biomarkers and therapeutic targets. Non-coding RNAs (ncRNAs), including microRNAs (miRNAs), long non-coding RNAs (lncRNAs), small interfering RNAs (siRNAs), and circular RNAs (circRNAs), have emerged as crucial regulators of breast luminal B cancer (BLBC) tumorigenesis and progression. Their subtype-specific expression and remarkable stability in body fluids make them promising candidates for minimally invasive biomarkers in clinical chemistry, enabling the early detection, prediction of prognosis, and monitoring of therapeutic response. Additionally, ncRNA-based therapeutics offer innovative strategies to overcome resistance and improve treatment efficacy. This review comprehensively synthesises the latest advances in understanding non-coding RNA (ncRNA) biology in breast luminal B-like cancer (BLBC), focusing on their roles in key oncogenic and tumour-suppressive pathways. We highlight the translational potential of ncRNAs as diagnostic and prognostic biomarkers and discuss emerging therapeutic applications alongside current challenges such as assay standardisation and delivery optimisation. The novelty of this review lies in its integrative approach, bridging molecular mechanisms with clinical implications to guide future research and clinical translation. We aim to provide a critical resource that supports the development of ncRNA-based precision medicine approaches to improve the management and outcomes of patients with basal-like breast cancer.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"576 ","pages":"Article 120408"},"PeriodicalIF":3.2,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immuno-PCR: Advancements, and applications for infectious diseases diagnosis 免疫pcr：进展及其在传染病诊断中的应用。

IF 3.2 3区医学

Clinica Chimica Acta Pub Date : 2025-05-31 DOI: 10.1016/j.cca.2025.120409

Ayushi Kaur Bedi, Monika Sharma, Sadhna Sharma

{"title":"Immuno-PCR: Advancements, and applications for infectious diseases diagnosis","authors":"Ayushi Kaur Bedi, Monika Sharma, Sadhna Sharma","doi":"10.1016/j.cca.2025.120409","DOIUrl":"10.1016/j.cca.2025.120409","url":null,"abstract":"<div><div>Immuno-polymerase chain reaction (I-PCR) is a hybrid technique that combines the specificity of immunoassays with the amplification power of polymerase chain reaction for highly sensitive detection of Infectious Diseases Biomarkers. Since its introduction, I-PCR has evolved through advances such as the introduction of universal streptavidin–biotin systems, nanoparticle-based amplification, magnetic bead-assisted formats and the adoption of real-time and digital PCR readouts. These improvements have enabled detection limits significantly lower than traditional immunoassays, enabling its effective application in detecting viral, bacterial, parasitic, prion, and fungal antigens, as well as host antibodies, with detection limits reaching femtogram levels. However, clinical translation remains restricted by assay complexity, background signal amplification, and the reliance on thermal cyclers and specialized equipment. Future developments focusing on simplifying assay workflows, optimizing DNA-antibody conjugation, implementing isothermal amplification methods, and designing multiplexed point-of-care diagnostic platforms are essential to overcome current limitations. Addressing these challenges through continued innovation is essential to completely harness the potential of I-PCR as a highly sensitive, rapid, and accessible diagnostic platform across diverse healthcare settings.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"576 ","pages":"Article 120409"},"PeriodicalIF":3.2,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA biosensors for detection of Alzheimer’s disease 用于阿尔茨海默病检测的MicroRNA生物传感器

IF 3.2 3区医学

Clinica Chimica Acta Pub Date : 2025-05-31 DOI: 10.1016/j.cca.2025.120410

Asma Vafadar , Mohammad Younesi , Mohammad Ehsan Maddahi , Sajad Ehtiati , Hossein Moradi Kazerouni , Faranak moradi khalaj , Hasan Ghasemi , Amir Savardashtaki

引用次数: 0