Clinica Chimica Acta最新文献

{"title":"Roles of HDL function and sphingosine-1-phosphate in vasospastic angina","authors":"Kei Sasaki ,&nbsp;Hirotaka Ezaki ,&nbsp;Yasuhiro Endo ,&nbsp;Daisuke Kudo ,&nbsp;Yumiko Suenaga ,&nbsp;Makoto Ayaori ,&nbsp;Masami Sakurada ,&nbsp;Katsunori Ikewaki","doi":"10.1016/j.cca.2025.120338","DOIUrl":"10.1016/j.cca.2025.120338","url":null,"abstract":"<div><h3>Background</h3><div>High-density lipoprotein cholesterol (HDL-C) levels are often reduced in patients with vasospastic angina (VSA), but the relevance of HDL functionality to VSA pathogenesis remains unclear. Cholesterol uptake capacity (CUC), a novel cell-free assay reflecting HDL-mediated cholesterol efflux, offers a practical measure of HDL functionality. In parallel, sphingosine-1-phosphate (S1P), an HDL-associated bioactive sphingolipid with vasoprotective properties, may also contribute to VSA. This study aimed to evaluate CUC and vasodilatory HDL components, including S1P, in patients with VSA.</div></div><div><h3>Methods and Results</h3><div>Seventy-seven patients, comprising 53 patients who underwent an acetylcholine (Ach) provocation test (32 VSA at diagnosis and 21 non-VSA) and an additional 24 VSA outpatients were included. Patients with VSA had higher triglyceride levels compared with non-VSA patients, but HDL-C levels were not different. Further analysis revealed that CUC was lower in VSA patients at diagnosis compared with non-VSA patients. Serum levels of sphingosine-1-phosphate (S1P), a sphingolipid associated with HDL, were elevated in the VSA group (1.74 ± 0.76 vs. 1.31 ± 0.49 µM; p &lt; 0.01). In the VSA outpatient and Non-VSA groups, S1P levels in crude analysis were significantly associated with VSA (OR = 3.14, 95 % CI: 1.25–7.88, p = 0.01). This association remained significant across all adjusted models (Models 1–4).</div></div><div><h3>Conclusions</h3><div>The present study found that CUC was a novel indicator of vasospasm-related HDL dysfunctionality and that S1P is a promising biomarker for treated patients with VSA. The cholesterol efflux pathway and sphingolipid metabolism could contribute to the etiology of vasospasm.</div><div>Study registration: This clinical study was registered with the University Hospital Medical Information Network Clinical Trials Registry (UMIN000020942).</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"574 ","pages":"Article 120338"},"PeriodicalIF":3.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143901955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soluble thrombomodulin in preeclampsia: Systematic review and meta-analysis","authors":"Jesiel Francisco de Jesus Fernandes Martins Lima ,&nbsp;Thaíse Emilia Moreira da Silva ,&nbsp;Ana Cristina dos Santos Lopes ,&nbsp;Victor Antonio Ferreira Freire ,&nbsp;Melina Barros-Pinheiro ,&nbsp;Patrícia Nessralla Alpoim","doi":"10.1016/j.cca.2025.120323","DOIUrl":"10.1016/j.cca.2025.120323","url":null,"abstract":"<div><div>Preeclampsia (PE) is a severe pregnancy complication associated with endothelial dysfunction. Soluble thrombomodulin (sTM) is a biomarker of endothelial injury, but its role in PE remains unclear. Although previous studies suggest elevated sTM levels in PE, inconsistencies in study designs and diagnostic criteria have led to conflicting findings. Our study addresses this gap through a systematic review and <em>meta</em>-analysis using standardized effect sizes and predefined severity groups to clarify the association between sTM and PE. This review followed the Cochrane Handbook and PRISMA guidelines and was registered in PROSPERO (CRD42023456919). A comprehensive search was conducted in MEDLINE/PubMed, EMBASE, and Web of Science for observational studies evaluating sTM levels in PE and normotensive controls. Eligible studies included case-control and cohort designs measuring sTM in plasma or serum. Two reviewers independently conducted data extraction and risk of bias assessment (Newcastle-Ottawa Scale). A random-effects <em>meta</em>-analysis estimated the Standardized Mean Difference (SMD) and 95 % confidence intervals (CI). Of 285 screened studies, 26 met inclusion criteria, and 20 were included in the <em>meta</em>-analysis. sTM levels were significantly higher in PE (SMD = 0.91, 95 % CI 0.3 to 1.53; I² = 94 %) with greater elevation in severe PE (SMD: 0.86, 95 % CI 0.55 to 1.16; I² = 50 %) than mild PE (SMD: 0.57, 95 % CI 0.08 to 1.06; I² = 82 %). High heterogeneity was observed, likely due to variations in inclusion criteria, measurement techniques, and diagnostic definitions. These findings support the potential of sTM as a biomarker for assessing PE severity. However, its clinical use remains limited by methodological heterogeneity and lack of standardized cutoff values. Further prospective studies are needed to validate its diagnostic and prognostic value and to enable its integration into clinical practice.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"574 ","pages":"Article 120323"},"PeriodicalIF":3.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Divergences between IFCC recommendations for internal quality control and ISO standards","authors":"Marco Pradella","doi":"10.1016/j.cca.2025.120334","DOIUrl":"10.1016/j.cca.2025.120334","url":null,"abstract":"<div><div>The Task Force on Global Lab Quality of the International Federation of Clinical Chemistry and Laboratory Medicine published its Recommendations on the topic of IQC, stating that they are derived from the ISO 15189:20222 standard. TF-GLQ deviates in several places from ISO 15189. Some references to CLSI documents are incorrect. Laboratories and Accreditation Bodies, however, may not deviate for the requirements of ISO 15189 from the original text of the ISO standard and the guides that remain compliant with it.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"574 ","pages":"Article 120334"},"PeriodicalIF":3.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143903910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expanding the clinical utility of reporter gene assay to infliximab biosimilars.","authors":"Dharmendra Jain, Igor Y Pavlov, Saša Čučnik, Julio C Delgado, Eszter Lázár-Molnár","doi":"10.1016/j.cca.2025.120337","DOIUrl":"https://doi.org/10.1016/j.cca.2025.120337","url":null,"abstract":"<p><strong>Objective: </strong>Infliximab and its biosimilars are approved for the treatment of inflammatory diseases. Monitoring of serum drug and anti-drug antibody levels is essential for managing patients with treatment failure. A Reporter Gene Assay (RGA), previously developed for infliximab, was validated for the measurement of biosimilars infliximab-dyyb and infliximab-abda, and detection of Anti-Drug Antibodies (ADA).</p><p><strong>Method: </strong>65 de-identified residual samples from patients receiving infliximab or its biosimilars, were tested. ELISA and cell-based Reporter Gene Assay (RGA) were performed to measure drug while a bridging ELISA and a modified RGA assay were performed to detect ADA.</p><p><strong>Results: </strong>Analysis of assay analytical parameters showed acceptable linearity (systematic error < 15 %), recovery (82-119 %), precision and reproducibility (coefficient of variation < 15 %) of the RGA assay for measuring biosimilars. Detection of ADA developed against infliximab or biosimilars showed a complete agreement (Cohen's k = 1; 95 % CI = 1.0 to 1.0) between the RGA assays using infliximab, versus infliximab-dyyb, or infliximab-abda as assay reagents.</p><p><strong>Conclusions: </strong>A functional cell-based reporter gene assay was validated for measuring serum concentrations of infliximab biosimilars and neutralizing antibodies. This study supports the bio- equivalency and cross-immunogenicity of parent drug and biosimilars and offers guidance for management of patients switching therapies between parent drug and biosimilars.</p>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":" ","pages":"120337"},"PeriodicalIF":3.2,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143982219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corin in cardiovascular diseases and stroke","authors":"Yue Gong ,&nbsp;Yichang Zhao ,&nbsp;Yang Li,&nbsp;Qianqian Wang,&nbsp;Chunkai Li,&nbsp;Keyi Song,&nbsp;Jinqiu Liu,&nbsp;Feifei Chen","doi":"10.1016/j.cca.2025.120343","DOIUrl":"10.1016/j.cca.2025.120343","url":null,"abstract":"<div><div>Corin is a type II transmembrane serine protease highly expressed in the heart. It plays a critical role in regulating fluid balance and improving cardiac function by converting pro-atrial natriuretic peptide into mature atrial natriuretic peptide. <em>CORIN</em> variants have been identified in patients with hypertension, heart failure, atrial fibrillation, and stroke. In vivo and in vitro, corin deficiency increases blood pressure and impairs cardiac function. Circulating soluble corin appears to have potential as a stable and specific biomarker for the risk prediction and prognostic assessment of cardiovascular diseases (CVDs) and stroke. In this review, we summarize the current knowledge on corin physiology and circulating corin and discuss cardiac corin expression and function in CVDs. In the future, corin-related therapeutic approaches to increase corin activity and raise corin levels may offer new opportunities to treat CVDs.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"574 ","pages":"Article 120343"},"PeriodicalIF":3.2,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosome biomarkers in breast cancer: Systematic review and meta-analysis","authors":"Yurou Kang ,&nbsp;Xiaoqing Cao ,&nbsp;Yujing Fan ,&nbsp;Yimin Li ,&nbsp;Tao Xu ,&nbsp;Qing Zhou ,&nbsp;Bangshun He","doi":"10.1016/j.cca.2025.120342","DOIUrl":"10.1016/j.cca.2025.120342","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;Breast cancer (BC) has become the primary cancer that threatens women’s health and life expectancy. Early diagnosis is crucial for effective treatment and favourable prognosis. As a non-invasive and valuable liquid biopsy method, exosomes are promising for the diagnosis and prognosis of BC. The aim of this &lt;em&gt;meta&lt;/em&gt;-analysis is to evaluate the diagnostic and prognostic value of exosome biomarkers in BC.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;A systematic search of relevant English literature was conducted in PubMed, Web of Science, and Cochrane library until August 2024 (diagnosis) and October 2024 (prognosis). QUADAS-2 and QUAPAS were used to assess the quality of the literature. Summary statistics and analyses of relevant effect sizes were conducted using STATA software. Subgroup analysis and sensitivity analysis were performed to identify potential sources of heterogeneity.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;For diagnosis, a total of 31 articles with 3,778 patients and 2,722 controls were included, the pooled sensitivity (SEN), specificity (SPE), and area under the receiver operating characteristic curve (AUC) of overall exosome biomarkers were 0.89 (95 %CI: 0.86–0.91), 0.87 (95 %CI: 0.85–0.90), and 0.94 (95 %CI: 0.92–0.96), respectively, indicating a high diagnostic value of exosomes in BC patients. Subgroup analysis suggested that miRNAs in exosomes exhibited better diagnostic value compared to proteins and non-miRNAs, the SEN, SPE, and AUC were 0.89 (95 %CI: 0.82–0.93), 0.86 (95 %CI: 0.80–0.90), and 0.92 (95 %CI: 0.90–0.94), respectively. Among all miRNAs, the pooled SEN, SPE, and AUC of miR-21 were 0.86 (95 %CI: 0.67–0.95), 0.90 (95 %CI: 0.78–0.96), and 0.95 (95 %CI: 0.92–0.96), respectively. The diagnostic efficiency was improved when biomarkers were combined as a panel (SEN 0.91 versus 0.87, SPE 0.89 versus 0.86, AUC 0.96 versus 0.91).&lt;/div&gt;&lt;div&gt;In terms of prognosis, we retrieved 14 articles with 2,781 patients. The pooled HR of overall survival (OS) and progression-free survival (PFS) were 1.41 (95 %CI: 0.92–1.90) and 4.39 (95 %CI: 1.87–6.91), respectively, indicating exosome biomarkers like soluble HLA-G, miR-1246, miR-155, and PSMA were a predictor of poor PFS in BC patients. Subgroup analysis in OS group revealed a significant association between the overexpression of exosome proteins (soluble HLA-G, AnxA2, NGF, CXCL13) and worse OS in BC patients (HR = 2.91, 95 %CI: 1.36–4.47). Similarly, the overexpression of miR-1246 and miR-155 was associated with worse PFS in BC patients (HR = 4.13, 95 %CI: 1.24–7.03). Moreover, when biomarkers were combined as a panel, the prognostic efficiency significantly improved in OS (HR = 4.05, 95 %CI: 2.26–5.84) outcome.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;div&gt;The &lt;em&gt;meta&lt;/em&gt;-analysis revealed that exosome miR-21 might serve as a promising diagnostic biomarker in BC. Dysregulated exosome proteins and miRNAs could predict poor OS and PFS outcomes, respectively.&lt;/div&gt;&lt;/","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"574 ","pages":"Article 120342"},"PeriodicalIF":3.2,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143903404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long non-coding RNAs in schizophrenia","authors":"Seyyed Navid Mousavinejad ,&nbsp;Seyed Ali Hosseini ,&nbsp;Mozhdeh Mohammadpour ,&nbsp;Felora Ferdosi ,&nbsp;Ehsan Dadgostar ,&nbsp;Siavash Abdolghaderi ,&nbsp;Seyyed Hossein Khatami","doi":"10.1016/j.cca.2025.120340","DOIUrl":"10.1016/j.cca.2025.120340","url":null,"abstract":"<div><div>Long noncoding RNAs (lncRNAs) have emerged as critical regulators of the pathogenesis of schizophrenia, a complex neuropsychiatric disorder influenced by genetic and environmental factors. These transcripts modulate gene expression through diverse mechanisms, including chromatin remodeling, transcriptional regulation, and posttranscriptional modifications. Recent studies have demonstrated significant alterations in lncRNA expression profiles in both the peripheral blood and brain tissues of schizophrenia patients, highlighting their potential as biomarkers and therapeutic targets. Dysregulated lncRNAs such as Gomafu, DISC-2, BDNF-AS, MEG3, and TUG1 have been linked to neurodevelopmental processes, inflammatory responses, and key synaptic plasticity pathways implicated in schizophrenia. Furthermore, antipsychotic treatments have been shown to influence lncRNA expression, which is correlated with symptom improvement. Sex-specific and age-related differences in lncRNA regulation further underscore their complexity and relevance to schizophrenia pathophysiology. This review consolidates current knowledge on the role of lncRNAs in schizophrenia, emphasizing their diagnostic potential.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"574 ","pages":"Article 120340"},"PeriodicalIF":3.2,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accelerating antimicrobial stewardship: An AI-CDSS approach to combating multidrug-resistant pathogens in the era of increasing resistance","authors":"Tai-Han Lin ,&nbsp;Hsing-Yi Chung ,&nbsp;Ming-Jr Jian ,&nbsp;Chih-Kai Chang ,&nbsp;Cherng-Lih Perng ,&nbsp;Feng-Yee Chang ,&nbsp;Chien-Wen Chen ,&nbsp;Hung-Sheng Shang","doi":"10.1016/j.cca.2025.120336","DOIUrl":"10.1016/j.cca.2025.120336","url":null,"abstract":"<div><h3>Objectives</h3><div>The World Health Organization has identified <em>Klebsiella pneumoniae</em> (KP) and <em>Pseudomonas aeruginosa</em> (PA) as significant public health threats owing to high antibiotic resistance. Traditional antibiotic susceptibility testing (AST) methods, crucial for determining the most suitable treatment regimen, typically require approximately 48–96 h (2–4 days) to yield results, including bacterial culture, rapid identification via matrix-assisted laser desorption/ionization–time of flight mass spectrometry (MALDI-TOF MS), and subsequent AST, which is too long for urgent clinical decisions. Here, we developed an artificial intelligence-clinical decision support system (AI-CDSS) utilizing machine learning to analyze MALDI-TOF MS data for antibiotic resistance prediction for these pathogens.</div></div><div><h3>Methods</h3><div>From 165,299 bacterial specimens, we selected 12,967 KP and 9,429 PA cases. Predictive models, the core of the AI-CDSS, were built using advanced machine learning algorithms, such as the random forest classifier (RFC) and light gradient boosting machine (LGBM), with GridSearchCV and 5-fold cross-validation optimization and robustness.</div></div><div><h3>Results</h3><div>Both the RFC and LGBM models demonstrated strong predictive performance, with area under the curve values predominantly ranging from 0.91 to 0.95. Sensitivity, specificity, positive predictive value, and negative predictive value primarily exceeded 80 %, ensuring reliable detection of resistance patterns. The AI-CDSS was designed to provide real-time, clinically actionable recommendations, enabling targeted antibiotic selection up to one day faster than conventional AST.</div></div><div><h3>Conclusions</h3><div>Integrating MALDI-TOF MS with machine learning in AI-CDSS significantly enhanced clinical decision-making, representing a major advancement in the rapid management of infectious diseases and antimicrobial stewardship.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"574 ","pages":"Article 120336"},"PeriodicalIF":3.2,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical correlations of plasma sphingosine-1-phosphate and sphingolipid key enzymes in severe dengue using laboratory and machine learning approach","authors":"Aashika Raagavi Jean Pierre ,&nbsp;Anand Kasirajan ,&nbsp;Siva Ranganathan Green ,&nbsp;Manikandan Sivaprakasam ,&nbsp;Rithanya Syam Sahaya Raj ,&nbsp;Jhansi Venkata Nagamani Josyula ,&nbsp;Srinivasa Rao Mutheneni ,&nbsp;Veni Subramanyam ,&nbsp;Agieshkumar Balakrishna Pillai","doi":"10.1016/j.cca.2025.120335","DOIUrl":"10.1016/j.cca.2025.120335","url":null,"abstract":"<div><h3>Background</h3><div>Sphingolipids are crucial for vascular integrity and cellular homeostasis, with recent studies highlighting their role in viral diseases.</div></div><div><h3>Objectives</h3><div>The study aimed to assess the plasma levels of sphingolipids, specifically Sphingosine-1-phosphate (S1P) and the key enzymes of sphingolipid metabolism: Sphingomyelin synthase (SMS1), Ceramide Kinase (CERK) and acid ceramidase (ASAH1) and its association with clinical outcomes of dengue.</div></div><div><h3>Methods</h3><div>This prospective cohort study had 102 dengue cases with 17 severe dengue (SD), 33 dengue with warning signs (DWW), 52 dengue without warning signs (DWOW) along with 10 each from other febrile illnesses and healthy controls. Blood was collected across febrile, defervescence, and convalescence phases. Plasma levels of S1P and the enzymes were measured using ELISA, mRNA using qRT-PCR. Predictive efficacy was determined using Support vector machine (SVM) models.</div></div><div><h3>Results</h3><div>Study showed a significant reduction in S1P levels across all dengue forms during febrile phases, with further decline in SD during the critical phase (P &lt; 0.05).mRNA levels of the enzymes were increasing during critical phase (P ≤ 0.001) with no significant difference noted in their respective protein levels. S1P and SMS1 levels correlated significantly with clinical severity indicators, including hematocrit, albumin, platelet count, and liver enzymes. SVM analysis identified CERK levels along with platelet count, HCT, and ALT as markers with high predictive accuracy for dengue severity.</div></div><div><h3>Conclusion</h3><div>The study reports an association of sphingolipids with dengue virulence, emphasizing the role of S1P metabolism in disease progression and plasma leakage, and highlighting the potential of targeting sphingolipids in managing severe dengue.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"574 ","pages":"Article 120335"},"PeriodicalIF":3.2,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EXSPECT: ELF Cross-comparison between serum and plasma collection tubes","authors":"Alexander Hung ,&nbsp;David Etoori ,&nbsp;Raakesh Modi ,&nbsp;Matthew Gee ,&nbsp;William Rosenberg","doi":"10.1016/j.cca.2025.120332","DOIUrl":"10.1016/j.cca.2025.120332","url":null,"abstract":"","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"574 ","pages":"Article 120332"},"PeriodicalIF":3.2,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}