Clinica Chimica Acta最新文献

{"title":"The diagnostic value of Renalase level in serum for chronic kidney disease with different stages","authors":"Siwen Yu ,&nbsp;Yanhua Wang ,&nbsp;Wenchao Fei ,&nbsp;Ke Xu ,&nbsp;Xiaolin Wu ,&nbsp;Yinghua Li","doi":"10.1016/j.cca.2025.120561","DOIUrl":"10.1016/j.cca.2025.120561","url":null,"abstract":"<div><h3>Objectives</h3><div>The study aimed to explore the role of renalase in chronic kidney disease (CKD) and establish a diagnostic cutoff value for chronic kidney disease (CKD).</div></div><div><h3>Methods</h3><div>Researchers analyzed serum renalase levels in 44 healthy adults and 250 CKD patients across stages I to V, along with other kidney function indicators like blood urea nitrogen (BUN), serum creatinine (CRE), uric acid (UA), Cystatin-C (Cys-C), β2-microglobulin (β2-mg), and estimated glomerular filtration rate (eGFR).</div></div><div><h3>Results</h3><div>Serum renalase concentrations progressively increased from stage I to V CKD patients, significantly correlating with advancing renal dysfunction. Strong correlations were found between renalase and age, CRE, eGFR, UA, Cys-C, BUN, β2-mg, and CKD stage (all <em>P</em> &lt; 0.001), but not with gender. Notably, the receiver operating characteristic (ROC) curve analysis highlighted stage II CKD, with an AUC of 0.980 for renalase, suggesting a diagnostic threshold of 6.595 μg/ml, offering 89.3 % sensitivity and 98.6 % specificity.</div></div><div><h3>Conclusions</h3><div>These results underscore serum renalase as a sensitive indicator for early-stage CKD diagnosis, complementing current biomarkers in detecting kidney impairment. Thus, renalase emerges as a promising novel biomarker need further investigation in more extensive cohorts for enhancing early CKD prediction capabilities.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"578 ","pages":"Article 120561"},"PeriodicalIF":2.9,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144906867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic utility of combined coagulation and inflammatory biomarkers in sepsis stratification: A retrospective study","authors":"Zhenzhen Ding ,&nbsp;Qian Li ,&nbsp;Chunyan Ma ,&nbsp;Hang Li","doi":"10.1016/j.cca.2025.120558","DOIUrl":"10.1016/j.cca.2025.120558","url":null,"abstract":"<div><h3>Objective</h3><div>The aim of this study is to evaluate the diagnostic utility of coagulation parameters—prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB), D-Dimer (D-D), prothrombin time activity (PTA), and international normalized ratio (INR) —in combination with inflammatory markers, including procalcitonin (PCT), C-reactive protein (CRP), interleukin-6 (IL-6), systemic inflammation index (SII), and lymphocyte-C-reactive protein ratio (LCR), for stratifying sepsis severity.</div></div><div><h3>Methods</h3><div>This retrospective study included 123 patients diagnosed with sepsis and confirmed by positive blood cultures between October 2020 and October 2024. A control group comprising 50 healthy individuals undergoing routine physical examinations was also included. Clinical data were collected and analyzed using SPSS 25.0. The sensitivity, specificity, receiver operating characteristic (ROC) curves, and area under the curve (AUC) values of each indicator were calculated to evaluate their diagnostic value.</div></div><div><h3>Results</h3><div>No statistically significant differences were observed in baseline demographic or clinical characteristics between the sepsis and control groups (<em>p</em> &gt; 0.05). Significant differences were identified between the groups in PT, APTT, D-D, PTA, INR, and CRP values (<em>p</em> &lt; 0.05).The correlation coefficients between different grades of sepsis (shock and non-shock) and PT, APTT,DD,INR,PTA,LCR, CRP were r = 0.328,r = 0.254,r = 0.272,r = 0.347,r = -0.331,r = -0.179,r = 0.200, respectively. P &lt; 0.05 is statistically significant,It is suggested that the classification is positively correlated with PT,APTT,DD,INR and CRP. PTA and LCR are negatively correlated, but the correlation in hierarchical diagnosis is low. Compared with sepsis group, the area under the curve (AUC) of coagulation index and inflammatory index in septic shock group was calculated. The area under the curve of PT in coagulation index was 0.695, the sensitivity was 53.2 %, and the specificity was 82.9 %. The area under the APTT curve was 0.651, the sensitivity was 66.0 %, and the specificity was 64.5 %. The area under the DD curve was 0.662, the sensitivity was 76.6 %, and the specificity was 57.9 %. Among the inflammatory indicators, the area under the CRP curve was 0.619, with a sensitivity of 63.8 % and a specificity of 63.2 %. ROC analysis revealed that the AUC of LCR in the septic shock group was 0.604, with a sensitivity of 66.0 % and specificity of 57.9 %. Combined detection of PT, LCR, and DD yielded an AUC of 0.8705, with a sensitivity of 74.5 % and specificity of 68.8 %.</div></div><div><h3>Conclusion</h3><div>Combined detection of coagulation markers such as PT with inflammatory markers including LCR, and DD may enhance the diagnostic accuracy for stratifying sepsis severity and identifying progression to septic shock.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"578 ","pages":"Article 120558"},"PeriodicalIF":2.9,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144892136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of thyroid peroxidase gene variant rs2175977 in subclinical hypothyroidism and autoimmune thyroid response","authors":"Larsa Naji Adam,&nbsp;Awat Mustafa Abbas","doi":"10.1016/j.cca.2025.120559","DOIUrl":"10.1016/j.cca.2025.120559","url":null,"abstract":"<div><h3>Aim</h3><div>Study investigated the association of the thyroid peroxidase (TPO) gene polymorphism with Subclinical hypothyroidism (SCH) risk.</div></div><div><h3>Methods</h3><div>A case-control study of 78 SCH patients SCH and 75 controls. Genomic DNA extracted from the blood, and the rs2175977 SNP in the Exon 8 of the TPO gene was genotyped through the PCR and Subsequent RFLP by SgrBI digestion. Statistics assessed genotype frequencies, allelic distributions, and associations with biochemical parameters.</div></div><div><h3>Results</h3><div>The GG genotype was significantly more in controls compared to SCH patients (p = 0.0039), while GC and CC genotypes were more prevalent in SCH. The C allele was significantly associated with increased SCH risk (p = 0.0006; OR = 0.3887). The GC and CC variant had significantly higher levels of Anti-TPO antibodies (p = 0.0429).</div></div><div><h3>Conclusion</h3><div>A TPO gene polymorphism, has been linked to increased risk of SCH and an elevated level of anti-TPO antibodies, indicating thyroid autoimmune disorder.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"578 ","pages":"Article 120559"},"PeriodicalIF":2.9,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144864242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring treatment response using serial PTX3 levels in chronic and invasive pulmonary aspergillosis","authors":"Shiang-Fen Huang ,&nbsp;Fu-Der Wang ,&nbsp;Chia-Chang Huang ,&nbsp;Kun-Ta Chou ,&nbsp;Yu-Chi Huang ,&nbsp;Ping-Feng Wu ,&nbsp;Chen-Te Lee ,&nbsp;Ying-Ying Yang","doi":"10.1016/j.cca.2025.120553","DOIUrl":"10.1016/j.cca.2025.120553","url":null,"abstract":"<div><h3>Background</h3><div>Pentraxin 3 (PTX3) is an innate immune biomarker involved in host defense against <em>Aspergillus</em> species. While elevated PTX3 levels have been observed in various infection states, its utility in monitoring treatment response in pulmonary aspergillosis remains underexplored.</div></div><div><h3>Methods</h3><div>We assessed serial PTX3 levels in total 216 patients with chronic pulmonary aspergillosis (CPA, <em>n</em> = 48) and invasive pulmonary aspergillosis (IPA, <em>n</em> = 48) before and after antifungal therapy. Comparative groups included controls (<em>n</em> = 69), COVID-19 patients (<em>n</em> = 36), and allergic bronchopulmonary aspergillosis (ABPA, <em>n</em> = 15). Serum PTX3 concentrations were measured by ELISA and analyzed in relation to treatment response. Neutrophil extracellular trap (NET) formation under various stimuli was evaluated and correlated with PTX3 levels to explore immune activation patterns.</div></div><div><h3>Results</h3><div>PTX3 levels were significantly elevated in CPA and IPA compared to controls. Among patients with serial samples, PTX3 levels declined significantly in favorable responders (from 299.0 [119.5–477.0] to 138.3 [80.9–386.1] pg/mL; p &lt; 0.05), but increased in unfavorable responders (from 177.1 [117.8–318.8] to 243.0 [169.5–488.4] pg/mL; <em>p</em> &lt; 0.05). PTX3 changes correlated with disease course and NET responses in a phenotype-specific manner.</div></div><div><h3>Conclusion</h3><div>Serial measurement of PTX3 may serve as a useful biomarker for monitoring treatment response in pulmonary aspergillosis. PTX3 dynamics reflect underlying immune activity and may aid in identifying disease progression or relapse.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"578 ","pages":"Article 120553"},"PeriodicalIF":2.9,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144882248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the role of Glial Fibrillary Acid Protein (GFAP) in Acute coronary syndrome","authors":"Luisa Agnello ,&nbsp;Giuseppe Vadalà ,&nbsp;Anna Maria Ciaccio ,&nbsp;Fabio Del Ben ,&nbsp;Cristina Madaudo ,&nbsp;Anna Masucci ,&nbsp;Martina Tamburello ,&nbsp;Roberta Vassallo ,&nbsp;Caterina Maria Gambino ,&nbsp;Alfredo Ruggero Galassi ,&nbsp;Marcello Ciaccio","doi":"10.1016/j.cca.2025.120556","DOIUrl":"10.1016/j.cca.2025.120556","url":null,"abstract":"","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"578 ","pages":"Article 120556"},"PeriodicalIF":2.9,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144860306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined clinical and genomic analysis uncovers neonatal-onset Wilson disease in two siblings with idiopathic cholestasis","authors":"Boudour Khabou ,&nbsp;Manel Guirat ,&nbsp;Manel Hsairi ,&nbsp;Lamia Gargouri ,&nbsp;Slim Charfi ,&nbsp;Mohamed Hadj Kacem ,&nbsp;Tahia Boudawara ,&nbsp;Hassen Kammoun ,&nbsp;Faiza Fakhfakh ,&nbsp;Hassen Hadj Kacem","doi":"10.1016/j.cca.2025.120557","DOIUrl":"10.1016/j.cca.2025.120557","url":null,"abstract":"<div><div>Wilson’s disease is an autosomal recessive disorder related to genetic defects in <em>ATP7B</em> gene and characterized by a hepatic and/or neurological impairment. This disease is often misdiagnosed on due to its phenotypic heterogeneity, supporting the importance of the genetic testing. In our study, we reported two brothers presenting with a chronic hepatopathy, classified as intrahepatic cholestasis with unknown etiology based on clinical explorations. A molecular screening through Whole-exome-sequencing was conducted, followed by sanger sequencing and co-segregation analysis in the family’s members. Generated data were the subject of <em>in silico</em> analysis. Results revealed two pathogenic heterozygous variants in <em>ATP7B</em> gene (p.Met665Ile/ p.Gly869Arg) in compound heterozygous state in the analyzed proband and his brother. Predictive data supported their effect on the stability at the RNA and protein levels. In line of these data, additional clinical explorations were ensured and the Leipzig score was assessed to establish the WD diagnosis. Moreover, we identified two additional heterozygous variants shared by both siblings in <em>CFTR</em> (p.Thr351Ser) and <em>PEX26</em> (p.Leu153Val), which may act as phenotype modifiers. Notably, both patients exhibited exocrine pancreatic insufficiency, raising the possibility of a contributory role for the <em>CFTR</em> variant in this atypical presentation. In conclusion, our study reveals a complex genetic background involving <em>ATP7B</em>, <em>CFTR</em>, and <em>PEX26</em>. While the <em>ATP7B</em> variants are responsible for WD, the additional variants may influence the age of onset and severity of the clinical phenotype. These findings underscore the value of high-throughput sequencing technologies in uncovering the molecular basis and phenotypic complexity of inherited diseases.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"578 ","pages":"Article 120557"},"PeriodicalIF":2.9,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144862161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunologic and genetic biomarkers in acute pancreatitis","authors":"Mohammad Younesi ,&nbsp;Mohammad Samare-Najaf ,&nbsp;Mohammad Karim Azadbakht ,&nbsp;Navid Jamali","doi":"10.1016/j.cca.2025.120555","DOIUrl":"10.1016/j.cca.2025.120555","url":null,"abstract":"<div><div>Acute pancreatitis (AP) is a severe inflammatory condition whose incidence continues to rise worldwide, causing significant mortality. The correct and immediate detection of medical conditions remains essential for delivering optimal patient care and better treatment results. Current diagnostic approaches exhibit limited specificity and sensitivity, hindering their ability to provide reliable prognostic information. Researchers have conducted extensive studies to discover and validate dependable biomarkers that help diagnose and evaluate the severity and predict the outcome of AP. The research investigations are organized into three distinct sections, which examine genetic elements such as non-coding RNAs and gene expression profiles, and immunological elements including cytokines, chemokines, acute phase reactants, and heterogeneous elements which encompass other relevant biomarkers. This review examines current research findings and potential methods that combine different biomarker panels to improve AP diagnosis, severity prediction, prognosis, and individualized patient management. Moreover, it comprehensively synthesizes immunologic, genetic, and classical biomarkers, offering an integrated clinical roadmap for AP management, and also evaluates classical biomarkers (amylase, lipase, trypsinogen-2) that remain clinical cornerstones for AP diagnosis.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"578 ","pages":"Article 120555"},"PeriodicalIF":2.9,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144852391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-invasive biomarkers in lung cancer","authors":"Ravikumar Baskar ,&nbsp;Suresh Saravanan ,&nbsp;Vishwar Devendiran ,&nbsp;Thirunavukkarasu Palaniyandi ,&nbsp;Natarajan Alamgudi Palaniappan ,&nbsp;Mugip Rahaman Abdul Wahab ,&nbsp;Hemapreethi Surendran ,&nbsp;Safia Obaidur Rab ,&nbsp;Mohd Saeed","doi":"10.1016/j.cca.2025.120552","DOIUrl":"10.1016/j.cca.2025.120552","url":null,"abstract":"<div><div>Lung cancer is the leading cause of cancer-related deaths worldwide, with its high mortality rate largely due to challenges in early detection. Blood- and tissue-based biomarkers are widely used for diagnosis; however, they often cause patient discomfort, require complex analytical methods, and rely heavily on expert interpretation for disease staging. Non-blood-based biomarkers from sputum, urine, saliva, sweat, and exhaled breath are emerging as effective alternatives for lung cancer diagnostics. These methods are non-invasive, cost-effective, and allow safer, repeated sampling, making them suitable for prognostic use. Urine contains biomarkers such as volatile organic compounds (VOCs) and circulating tumour DNA, providing valuable metabolic insights. Saliva offers proteins, microRNAs, and exosomal biomarkers that reflect systemic disease-related changes. Sputum contains diverse molecular markers that aid early detection. Exhaled breath carries microRNAs and VOCs, representing another promising diagnostic route. Overall, non-invasive biomarker platforms significantly reduce procedural risks and enable earlier lung cancer detection. Their advancement holds substantial potential to improve patient outcomes globally.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"578 ","pages":"Article 120552"},"PeriodicalIF":2.9,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144852390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of fibrin in high sensitivity cardiac troponin (Hs-cTn) detection: Implications for quality control","authors":"Chijioke Noris Ezinwa","doi":"10.1016/j.cca.2025.120551","DOIUrl":"10.1016/j.cca.2025.120551","url":null,"abstract":"<div><div>High-sensitivity troponin (hs-cTn) assays are crucial in the timely diagnosis of acute myocardial infarction (AMI). However, the presence of fibrin clots, among other interfering factors, can lead to false-positive results, undermining diagnostic accuracy. This critical literature review evaluates the implications of fibrin clot detection in hs-cTn testing, emphasising the need for robust quality control measures in emergency cardiac testing. Through this review, it was shown that biomedical scientists must prevent activities during the pre-analysis stage that may lead to fibrin formation. When such situations become inevitable due to various reasons, including patients’ predispositions, several steps were outlined to confirm the diagnostic accuracy of hs-cTn in myocardial infarction. The importance of technological advancements in detecting fibrin and other potential sources of cross-reactivity or interference was also identified through a comparative analysis of some auto-analysers, proposing possible solutions to address these pitfalls as a means of promoting efficiency and quality assurance in the diagnosis of myocardial infarction through high-sensitivity troponin assays. It further exposed the limitations of these digital analysers and how they cannot independently determine the procedural and outcome quality of diagnostic medicine.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"578 ","pages":"Article 120551"},"PeriodicalIF":2.9,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomes in preeclampsia","authors":"Asma Vafadar ,&nbsp;Zahra Heidari ,&nbsp;Pedram Bolbolizadeh ,&nbsp;Damoun Razmjoue ,&nbsp;Sajad Ehtiati ,&nbsp;Amir Savardashtaki","doi":"10.1016/j.cca.2025.120549","DOIUrl":"10.1016/j.cca.2025.120549","url":null,"abstract":"<div><div>Preeclampsia (PE) is a multifactorial pregnancy disorder marked by new-onset hypertension after 20 weeks of gestation, often accompanied by proteinuria or organ dysfunction. Affecting 4–6 % of pregnancies globally, PE remains a leading cause of maternal and fetal morbidity. While its precise etiology is not fully understood, growing evidence implicates extracellular vesicles—particularly exosomes—in its pathogenesis. These nanoscale vesicles facilitate intercellular communication by transporting bioactive molecules, including proteins, lipids, and non-coding RNAs (ncRNAs) such as miRNAs, lncRNAs, and circRNAs. In PE, placental exosomes show altered profiles that contribute to endothelial dysfunction, immune imbalance, and impaired angiogenesis. This review synthesizes current knowledge on exosome biogenesis, their diagnostic utility—especially exosomal ncRNAs as non-invasive biomarkers—and emerging therapeutic strategies, including engineered exosomes for targeted delivery. Our findings highlight the promise of exosomes as precision medicine tools in PE management.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"578 ","pages":"Article 120549"},"PeriodicalIF":2.9,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144830977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}