Clinica Chimica Acta最新文献

筛选
英文 中文
Diagnostic and prognostic utility of endocan in suspected myocardial infarction: an international cohort study. 内啡肽在疑似心肌梗死中的诊断和预后应用:一项国际队列研究。
IF 2.9 3区 医学
Clinica Chimica Acta Pub Date : 2026-01-01 Epub Date: 2025-07-25 DOI: 10.1016/j.cca.2025.120510
Franja Dugar, Pedro Lopez-Ayala, Luca Koechlin, Paolo Bima, Jonas Glaeser, Carlos Spagnuolo, Luca Crisanti, Clara Wick, Karin Wildi, Emel Kaplan, Ivo Strebel, Oscar Miro, Francisco Javier Martin-Sanchez, Michael Christ, Beata Morawiec, Maria Rubini Gimenez, Thomas Nestelberger, Jasper Boeddinghaus, Christian Mueller
{"title":"Diagnostic and prognostic utility of endocan in suspected myocardial infarction: an international cohort study.","authors":"Franja Dugar, Pedro Lopez-Ayala, Luca Koechlin, Paolo Bima, Jonas Glaeser, Carlos Spagnuolo, Luca Crisanti, Clara Wick, Karin Wildi, Emel Kaplan, Ivo Strebel, Oscar Miro, Francisco Javier Martin-Sanchez, Michael Christ, Beata Morawiec, Maria Rubini Gimenez, Thomas Nestelberger, Jasper Boeddinghaus, Christian Mueller","doi":"10.1016/j.cca.2025.120510","DOIUrl":"10.1016/j.cca.2025.120510","url":null,"abstract":"<p><strong>Background: </strong>The possible clinical utility of endocan, a novel inflammatory biomarker involved in the initiation and progression of atherosclerosis, is largely unknown. We aimed to evaluate its diagnostic and prognostic performance in chest pain patients presenting to the emergency department (ED).</p><p><strong>Methods: </strong>We prospectively enrolled patients presenting with suspected myocardial infarction (MI) to the ED in an international multicenter study. Endocan, high-sensitivity C-reactive protein (hs-CRP), and high-sensitivity cardiac troponin T (hs-cTnT) were measured in blood samples obtained at presentation. Final diagnoses were centrally adjudicated by two independent cardiologists applying the 4th universal definition of MI and current guidelines. Non-ST-elevation MI (NSTEMI) was the diagnostic endpoint and 5-year cardiovascular death was the primary prognostic endpoint.</p><p><strong>Results: </strong>Among 4728 patients, 843 (17.8 %) had NSTEMI. The diagnostic discrimination of endocan for NSTEMI was low (area under the curve (AUC) 0.585 [95 % CI: 0.563-0.607]. Its combination with hs-cTnT (0.939 [95 % CI: 0.931-0.947]) did not improve the discriminative performance of hs-cTnT alone (0.937 [95 % CI: 0.930-0.950]). Long-term prognostic accuracy of endocan was higher versus hs-CRP, but lower versus hs-cTnT (AUC 0.730 [0.710-0.760] vs 0.650 [0.620-0.680] vs 0.810 [0.790-0.830], respectively). Endocan was associated with an increased 5-year risk for cardiovascular mortality. However, it did not provide relevant incremental prognostic value when added on top of a base model that included SCORE2 risk factors and hs-cTnT.</p><p><strong>Conclusion: </strong>Endocan has a low diagnostic accuracy for NSTEMI, and moderate long-term prognostic accuracy for cardiovascular death.</p><p><strong>Clinical trial registration: </strong>ClinicalTrials.gov number, NCT00470587, https://clinicaltrials.gov/ct2/show/NCT00470587https://clinicaltrials.gov/ct2/show/NCT00470587.</p>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":" ","pages":"120510"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144728330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Aptamer biosensors in early breast cancer detection. 适体生物传感器在早期乳腺癌检测中的应用。
IF 2.9 3区 医学
Clinica Chimica Acta Pub Date : 2026-01-01 Epub Date: 2025-08-06 DOI: 10.1016/j.cca.2025.120536
Hamdi Nsairat, Omer Qutaiba B Allela, Abdulkareem Shareef, S Renuka Jyothi, Priya Priyadarshini Nayak, Ashish Singh Chauhan, Hayder Naji Sameer, Ahmed Yaseen, Zainab H Athab, Mohaned Adil
{"title":"Aptamer biosensors in early breast cancer detection.","authors":"Hamdi Nsairat, Omer Qutaiba B Allela, Abdulkareem Shareef, S Renuka Jyothi, Priya Priyadarshini Nayak, Ashish Singh Chauhan, Hayder Naji Sameer, Ahmed Yaseen, Zainab H Athab, Mohaned Adil","doi":"10.1016/j.cca.2025.120536","DOIUrl":"10.1016/j.cca.2025.120536","url":null,"abstract":"<p><p>Breast cancer, a leading cause of cancer-related mortality among women, necessitates the development of sensitive and specific diagnostic tools for early detection and personalized treatment. An aptamer-based biosensor (aptasensor) is a biosensor that utilizes aptamers as its biorecognition element such as single-stranded DNA or RNA molecules that are specifically selected to bind to a target molecule with high affinity. Aptasensors have emerged as promising alternatives to traditional methods, offering advantages such as high affinity, specificity, and ease of synthesis. This review provides an overview of recent advancements in aptasensor technology for breast cancer diagnostics, focusing on the detection of key biomarkers. In this review, first after an overview to different types of aptasensors, we discuss the different type of aptamer immobilization methods on sensor surfaces using covalent, adsorption, and thiol-based self-assembly techniques. Furthermore, we present different aptasensor platforms, including electrochemical and optical approaches, highlighting their design principles, performance characteristics, and clinical applications.</p>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":" ","pages":"120536"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144798323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Proteomic approaches for biomarker discovery and clinical applications in autoimmune diseases. 自身免疫性疾病中生物标志物发现和临床应用的蛋白质组学方法。
IF 2.9 3区 医学
Clinica Chimica Acta Pub Date : 2026-01-01 Epub Date: 2025-08-06 DOI: 10.1016/j.cca.2025.120533
Sultan Ayesh Mohammed Saghir, Amir M Al Hroob, Salah A Alshehade, Sulaiman Alnaimat, Nuha A Al Yousfi, Sarah Ahmad Bahjat Al-Rawashdeh, Mohammad Ahmad Al Rawashdeh, Mohammed Abdullah Alshawsh
{"title":"Proteomic approaches for biomarker discovery and clinical applications in autoimmune diseases.","authors":"Sultan Ayesh Mohammed Saghir, Amir M Al Hroob, Salah A Alshehade, Sulaiman Alnaimat, Nuha A Al Yousfi, Sarah Ahmad Bahjat Al-Rawashdeh, Mohammad Ahmad Al Rawashdeh, Mohammed Abdullah Alshawsh","doi":"10.1016/j.cca.2025.120533","DOIUrl":"10.1016/j.cca.2025.120533","url":null,"abstract":"<p><p>Autoimmune diseases (ADs) impact approximately 3% of the global population. It encompasses more than 80 chronic, often debilitating conditions resulting from immune system defects that lead the body to attack its tissues. Although many ADs are rare, their prevalence is rising. Despite advancements, ADs diagnosis and classification still rely heavily on clinical examination combined with conventional laboratory testing and imaging. Emerging proteomic screening, technologies now offer the potential to identify unique biomarkers for more precise diagnosis, classification, and treatment decisions. Protein profiling, an advancing area within proteomics, provides unparalleled insights into biological processes underlying these diseases. Thus, this review highlights recent developments in proteomic and genetic profiling for ADs pathogenesis, diagnosis, and treatment challenges, focusing on the clinical utility of proteomic techniques in prognosis, diagnosis, and therapeutic guidance. Notably, discovering new biomarkers through proteomic screening is crucial for creating robust multi-parameter assays, which enhance diagnostic accuracy and treatment decisions in ADs.</p>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":" ","pages":"120533"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Advanced aptasensor technologies for sensitive detection of 17β-Estradiol. 17β-雌二醇灵敏检测的先进适体传感器技术。
IF 2.9 3区 医学
Clinica Chimica Acta Pub Date : 2026-01-01 Epub Date: 2025-08-05 DOI: 10.1016/j.cca.2025.120534
Yanan Cao, Mingshu Zhou, Hua Li
{"title":"Advanced aptasensor technologies for sensitive detection of 17β-Estradiol.","authors":"Yanan Cao, Mingshu Zhou, Hua Li","doi":"10.1016/j.cca.2025.120534","DOIUrl":"10.1016/j.cca.2025.120534","url":null,"abstract":"<p><p>The detection of 17β-estradiol (E2), a potent endocrine-disrupting compound, is critical for both environmental monitoring and biomedical diagnostics. Traditional detection methods often suffer from limitations in sensitivity, selectivity, and cost-effectiveness. Aptasensors, which utilize aptamers as biorecognition elements, offer promising alternatives because of their high specificity, stability, and adaptability. This paper explores recent advancements in aptasensor technologies for E2 detection, highlighting optical, electrochemical, and surface-enhanced Raman scattering (SERS)-based platforms. The integration of nanomaterials such as gold nanoparticles, carbon dots, and conductive polymers significantly enhances sensor performance, achieving ultralow detection limits and broad dynamic ranges. By leveraging these innovations, aptasensors provide scalable solutions for real-time monitoring of E2 in environmental, food, and clinical samples, paving the way for improved endocrine regulation and public health safety.</p>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":" ","pages":"120534"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144798322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Blood collected on dry blood spots is fit for newborn screening of sickle cell disease (SCD) by different analytical systems. 干血点采集的血液适合用不同的分析系统筛查新生儿镰状细胞病(SCD)。
IF 2.9 3区 医学
Clinica Chimica Acta Pub Date : 2026-01-01 Epub Date: 2025-08-05 DOI: 10.1016/j.cca.2025.120528
Renata Paleari, Matteo Vidali, Roberta Rolla, Ferruccio Ceriotti, Massimiliano Ammirabile, Andrea Mosca
{"title":"Blood collected on dry blood spots is fit for newborn screening of sickle cell disease (SCD) by different analytical systems.","authors":"Renata Paleari, Matteo Vidali, Roberta Rolla, Ferruccio Ceriotti, Massimiliano Ammirabile, Andrea Mosca","doi":"10.1016/j.cca.2025.120528","DOIUrl":"10.1016/j.cca.2025.120528","url":null,"abstract":"<p><p>Sickle cell disease (SCD) is a severe hereditary hemoglobinopathy with the highest burden in sub-Saharan Africa. Timely diagnosis via newborn screening is critical to enabling low-cost, life-saving interventions, yet its implementation remains inconsistent worldwide. This study assessed the performance and analytical stability of dried blood spot (DBS) samples collected on Guthrie cards for quantifying hemoglobin S (Hb S), using three high-performance liquid chromatography (HPLC) platforms and one capillary electrophoresis system. Simulated neonatal samples at three Hb S concentrations (non-carrier, carrier, and affected) were analyzed at three timepoints (immediate, 1 week, and 2 weeks post-collection). Across all methods, Hb S quantification was highly reproducible, with inter-timepoint variation remaining within the predefined critical threshold for the vast majority of the measurements. While minor discrepancies were observed for fetal hemoglobin (Hb F), all methods correctly classified samples for SCD screening purposes. These findings confirm that Guthrie card-based DBS is a robust and practical matrix for Hb S detection, suitable for transport and delayed analysis-even across different analytical platforms. Limitations include the use of spiked rather than native SCD neonatal samples and ambient-temperature shipping. Nonetheless, the results support broader adoption of DBS in SCD screening programs, particularly in low-resource or decentralized settings, and highlight the need for further standardization of Hb F quantification.</p>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":" ","pages":"120528"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144774697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sensitive and visual detection of SMA using RPA-Cas12a one-step assay with ssDNA-modified crRNA. 利用RPA-Cas12a一步法和ssdna修饰的crRNA对SMA进行灵敏和视觉检测。
IF 2.9 3区 医学
Clinica Chimica Acta Pub Date : 2026-01-01 Epub Date: 2025-08-06 DOI: 10.1016/j.cca.2025.120537
Qinyi Huang, Changzhi Xu, Shiqi Liu, Haihong Shi, XiaoBin Zhong, Shumin Zhao, Fengxiang Wei, Lvyuan Fan, Cui Wang, Yuanqing Li, Jia Tang
{"title":"Sensitive and visual detection of SMA using RPA-Cas12a one-step assay with ssDNA-modified crRNA.","authors":"Qinyi Huang, Changzhi Xu, Shiqi Liu, Haihong Shi, XiaoBin Zhong, Shumin Zhao, Fengxiang Wei, Lvyuan Fan, Cui Wang, Yuanqing Li, Jia Tang","doi":"10.1016/j.cca.2025.120537","DOIUrl":"10.1016/j.cca.2025.120537","url":null,"abstract":"<p><p>The irreversibility and lethality of Spinal Muscular Atrophy (SMA) underscore the urgency of newborn screening, as diagnostic delay in neonates causes irreversible motor neuron degeneration and poor outcomes. Current SMA detection methods are hindered by high costs, dependence on specialized equipment, and technical complexity, restricting their implementation in primary care setting. Here, we proposed a fast and sensitive SMA-(Recombinase Polymerase Amplification) RPA-Cas12a detection assay based on suboptimal protospacer adjacent motif (sPAM) and 3'-end ssDNA-modified crRNA, named SPSMC. The crRNA is designed based on the sPAM to enhance the specificity of SMN1 gene detection. The competition between RPA and Cas12a digestion for target DNA was resolved by using 3'-end ssDNA-modified crRNA. With ALB as a reference gene, this method can detect DNA at concentrations as low as 1.8 pM within 1 h. The sensitivity and specificity of the proposed method in differentiating SMA patients from non-SMA individuals were both 100 %. This strategy has been used for the detection of the SMN1 gene, which saves time, reduces contamination risks, and offers new possibilities for future point-of-care screening of SMA. In addition, the SPSMC system was successfully adapted to SMA lateral flow assay format and validated using 66 clinical samples, demonstrating 100 % sensitivity and specificity. The method is straightforward to perform, requires no bulky equipment, maintains full portability, and is more suitable for large-scale neonatal screening scenarios compared with traditional methods.</p>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":" ","pages":"120537"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144798326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exosomal non-coding RNA biomarkers in traumatic brain injury. 外伤性脑损伤的外泌体非编码RNA生物标志物。
IF 2.9 3区 医学
Clinica Chimica Acta Pub Date : 2026-01-01 Epub Date: 2025-08-07 DOI: 10.1016/j.cca.2025.120544
Pulatov Sadriddin, Babajanova Umida, Matyakubov Izzat, Urinov Muso, Toxtiyev Jaxongirbek, Akhmedov Shakhboskhan, Narkulova Soxiba, Muradova Emma, Bekchanova Madina, Shodmonov Iskandar, Kasimov Kabil Kasimovich, Matrizayeva Gulnara Djumaniyazovna, Kurbaniyazova Madina Zafajanovna
{"title":"Exosomal non-coding RNA biomarkers in traumatic brain injury.","authors":"Pulatov Sadriddin, Babajanova Umida, Matyakubov Izzat, Urinov Muso, Toxtiyev Jaxongirbek, Akhmedov Shakhboskhan, Narkulova Soxiba, Muradova Emma, Bekchanova Madina, Shodmonov Iskandar, Kasimov Kabil Kasimovich, Matrizayeva Gulnara Djumaniyazovna, Kurbaniyazova Madina Zafajanovna","doi":"10.1016/j.cca.2025.120544","DOIUrl":"10.1016/j.cca.2025.120544","url":null,"abstract":"<p><p>Traumatic brain injury (TBI) poses a significant global health challenge, leading to high mortality and morbidity rates. Despite extensive research, effective diagnostic and therapeutic strategies remain limited. This review explores the emerging roles of exosomal non-coding RNAs (ncRNAs) as biomarkers and therapeutic agents in TBI. Exosomes, small extracellular vesicles secreted by various cell types, facilitate intercellular communication and carry diverse ncRNAs that modulate gene expression and cellular functions. The pathogenesis of TBI involves complex inflammatory cascades, in which exosomal ncRNAs play pivotal roles in neuroinflammation, neuronal apoptosis, and secondary injury mechanisms. Key ncRNAs, including long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), have been identified as potential biomarkers for diagnosing TBI and predicting outcomes. Additionally, exosomal ncRNAs derived from mesenchymal stem cells (MSCs) show promise in promoting neuroprotection and enhancing recovery through various mechanisms. The review highlights the potential of exosomal ncRNAs in addressing critical unmet needs in TBI management, emphasizing their stability, accessibility, and relevance to disease pathophysiology. However, challenges in standardization, validation, and regulatory pathways must be addressed to facilitate their clinical application. This comprehensive examination underscores the transformative potential of exosomal ncRNAs in TBI diagnosis and treatment, paving the way for future research and clinical innovations.</p>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":" ","pages":"120544"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ethnicity-based variations in biological reference interval- A systematic scoping review. 基于种族的生物参考区间差异——系统范围综述。
IF 2.9 3区 医学
Clinica Chimica Acta Pub Date : 2026-01-01 Epub Date: 2025-08-07 DOI: 10.1016/j.cca.2025.120539
Sasidharan Sivakumar, Ishika Makhija, Ruchika Bhagat, Saanvi Maurya, Nabendu Sekhar Chatterjee, Savita Bansal, Nilesh Chandra
{"title":"Ethnicity-based variations in biological reference interval- A systematic scoping review.","authors":"Sasidharan Sivakumar, Ishika Makhija, Ruchika Bhagat, Saanvi Maurya, Nabendu Sekhar Chatterjee, Savita Bansal, Nilesh Chandra","doi":"10.1016/j.cca.2025.120539","DOIUrl":"10.1016/j.cca.2025.120539","url":null,"abstract":"<p><strong>Background: </strong>Biological reference intervals (RIs) are fundamental tools in clinical diagnostics, traditionally derived from geographically and ethnically homogeneous populations, predominantly of Western origin. Such generalized RIs often fail to account for variations arising from genetic, environmental, and lifestyle factors, which can impact clinical decision-making and contribute to health inequities, particularly in countries across Africa, India, and many other Southeast Asian nations. This scoping review investigates ethnicity-based variations in RIs for a range of biomarkers to highlight the importance of population-specific RIs.</p><p><strong>Methods: </strong>Adhering to the Joanna Briggs Institute (JBI) guidelines, this scoping review examined the literature on ethnicity-based RI variations across multiple biomarkers, including Von Willebrand factor, C-reactive protein, thyroid-stimulating hormone, albumin, creatinine, and more. Studies were identified via searches in MEDLINE (via PubMed), Embase, Scopus, and Web of Science up to December 30, 2024. Eligibility was determined using the Population-Concept-Context (PCC) framework, focusing on observational studies analysing ethnicity-specific differences. Data extraction and charting were performed using CADIMA software, with independent review by two authors to ensure consistency.</p><p><strong>Results: </strong>Out of 4,514 articles, a total of 15 studies met the inclusion criteria, encompassing multi-ethnic populations. Significant variations in biomarker levels were observed across ethnic groups, highlighting the inadequacy of generalized RIs. Notable differences included variations in lipid profiles, vitamin B12, and anti-Mullerian hormone levels. Regional distribution analysis highlighted gaps in research from underrepresented ethnic populations.</p><p><strong>Conclusion: </strong>This review emphasizes the critical need for ethnicity-specific RIs to improve diagnostic accuracy and promote equitable healthcare outcomes. Further research should focus on developing robust methodologies for establishing inclusive and representative RIs.</p>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":" ","pages":"120539"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of Pseudomonas aeruginosa in 5,021 hospitalized pediatric patients presenting with acute respiratory tract infections utilizing targeted next-generation sequencing. 利用靶向新一代测序技术检测5021例急性呼吸道感染儿科住院患者的铜绿假单胞菌
IF 2.9 3区 医学
Clinica Chimica Acta Pub Date : 2026-01-01 Epub Date: 2025-08-06 DOI: 10.1016/j.cca.2025.120529
Chunyun Fu, Junming Lu, Xiangjun Lu, Yanhua Feng, Huan Zhang, Wenting Tang, Qiang Huang, Ya Huang, Lishai Mo, Huiping Huang, Qifei Li, Jie Tan
{"title":"Detection of Pseudomonas aeruginosa in 5,021 hospitalized pediatric patients presenting with acute respiratory tract infections utilizing targeted next-generation sequencing.","authors":"Chunyun Fu, Junming Lu, Xiangjun Lu, Yanhua Feng, Huan Zhang, Wenting Tang, Qiang Huang, Ya Huang, Lishai Mo, Huiping Huang, Qifei Li, Jie Tan","doi":"10.1016/j.cca.2025.120529","DOIUrl":"10.1016/j.cca.2025.120529","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to assess the infection status and clinical manifestations of Pseudomonas aeruginosa (P.a.) in hospitalized children with respiratory tract infections.</p><p><strong>Methods: </strong>Between April 2021 and November 2023, a total of 5,021 hospitalized children with acute respiratory tract infections were collected at the Guangxi Maternal and Child Health Hospital. tNGS was used to detect pathogens in their respiratory samples.</p><p><strong>Results: </strong>Among the 5,021 hospitalized children with acute respiratory tract infections, P.a. was detected in 113 cases, with a detection rate of 2.25 %. The infected children were mainly infants under 1 year old, and males were predominant. Among the 113 P.a. positive cases, there was 1 case of single P.a. infection and 112 cases of co-infection with other pathogens. In infection patterns, the P.a.-bacteria-virus co-infection is relatively common. A total of 53 different pathogens were identified in children with mixed P. aeruginosa infections, with Human Herpesvirus 5 (observed in 40 cases) being the most prevalent, followed by Acinetobacter baumannii (39 cases) and Mycoplasma pneumoniae (24 cases). Among children with P. aeruginosa infection, the most common respiratory complications included sinusitis, respiratory failure, and pulmonary consolidation. In contrast, the most frequent complications affecting other systems were electrolyte disturbances, gastrointestinal dysfunction, anemia, and myocardial damage. The median duration of hospitalization for the 113 children was 8 days. Out of 113 children with P.a. infection, 55 cases (48.67 %) required respiratory support and 18 cases (15.93 %) required intensive care unit (ICU) admission.</p><p><strong>Conclusion: </strong>The detection rate of P.a. in hospitalized children with respiratory tract infections was 2.25 %. Almost all hospitalized children with P.a. respiratory tract infections had co-infections with other pathogens. The median duration of hospitalization was 8 days. 15.93 % of hospitalized children with P.a. infection required intensive care.</p>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":" ","pages":"120529"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144798324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biological sample storage in biomedical research. 生物医学研究中生物样品的保存。
IF 2.9 3区 医学
Clinica Chimica Acta Pub Date : 2026-01-01 Epub Date: 2025-08-06 DOI: 10.1016/j.cca.2025.120538
Claudia Carranza, Esmeralda Juárez, Laura E Carreto-Binaghi, Rodolfo L Chávez-Domínguez, Montserrat A García-Ramos, Mercedes Viettri, I A Corona-Galvan, Horacio Zamudio-Meza, Edgar Reyna-Rosas, Milton Nieto-Ponce, Andrea Palencia-Reyes, Carlos Blancas-Ruíz, Martha Torres
{"title":"Biological sample storage in biomedical research.","authors":"Claudia Carranza, Esmeralda Juárez, Laura E Carreto-Binaghi, Rodolfo L Chávez-Domínguez, Montserrat A García-Ramos, Mercedes Viettri, I A Corona-Galvan, Horacio Zamudio-Meza, Edgar Reyna-Rosas, Milton Nieto-Ponce, Andrea Palencia-Reyes, Carlos Blancas-Ruíz, Martha Torres","doi":"10.1016/j.cca.2025.120538","DOIUrl":"10.1016/j.cca.2025.120538","url":null,"abstract":"<p><p>Despite the central role of biological samples in biomedical discovery, many research laboratories operate without robust sample storage systems. This compromises sample integrity, reproducibility, biosafety, and the long-term utility of the data. Implementing structured sample storage policies and infrastructure is crucial for maintaining scientific quality, ensuring regulatory compliance, and promoting responsible research conduct. It would be highly beneficial for any research laboratory to transition from sample storage to the implementation of a formal biobank. Biobanks constitute a critical infrastructure for biomedical research, as they systematically store biological specimens and databases, which are essential for maximizing utility in clinical investigations and facilitating international collaborative efforts. Most countries lack biobank-specific regulatory frameworks, which limits the ability to implement concurrent initiatives in line with international standards. Integrating quality management systems is essential for ensuring specimen quality and security. In this study, we analyzed the importance of the sample storage conditions and the steps required to transition to a formal biobank. We proposed a guide for doing so in the absence of a regulatory framework, based on the ISO 20387:2018 model, and included an example of successful implementation. We integrated environmental sustainability considerations and inter-institutional networking as a strategy for enhancing operational efficiency. Transforming an internal sample storage system into a formal biobank is a strategic and scientific advancement. It ensures that biological materials are managed with the rigor, transparency, and ethical responsibility required by contemporary biomedical research. By doing so, research institutions strengthen their capacity for innovation and collaboration.</p>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":" ","pages":"120538"},"PeriodicalIF":2.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信