Establishing reference intervals for venous whole blood micronutrients in the healthy older Chinese population via dried blood spot technology.

IF 2.9 3区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Clinica Chimica Acta Pub Date : 2025-10-19 DOI:10.1016/j.cca.2025.120674

Zehao Wang, Huilian Duan, Xiaocui Cheng, Cheng Cheng, Wen Li, Fei Ma, Ruikun He, Zhongxia Li, Mengtong Yang, Di Wang, Zhenghua Huang, Yongjie Chen, Guowei Huang

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引用次数: 0

Abstract

Background: The reference intervals (RIs) for venous whole blood micronutrients are instrumental in evaluating nutritional status within populations. However, research on establishing micronutrient RIs using the simple and efficient dried blood spot (DBS) technique remains scarce. Consequently, this study aims to establish RIs for venous whole blood micronutrients among healthy Chinese older individuals via DBS.

Methods: Participants were recruited from the general community of Baodi District, Tianjin, northern China. Healthy populations were identified based on the recommendations of the Clinical and Laboratory Standards Institute guidelines, physical signs (body mass index and waist circumference), and blood biochemical parameters (serum lipid status and hemoglobin). The micronutrient levels were measured via the DBS technique. For normally distributed data, the RI was calculated as the mean ± 2SD. For non-normally distributed data, the RI is calculated as the 2.5th to 97.5th percentiles.

Results: This study established RIs for 8 vitamins (vitamins A, D, E, B₁, B₂, B₃, B₆, B₉) and 5 minerals (Mg, Cu, Fe, Zn, Se) in healthy older adults. For males, the RIs are as follows: VA (157.76-828.81 ng/mL); VD (8.89-58.32 ng/mL); VE (1.84-13.78 μg/mL); VB₁ (0.19-5.36 ng/mL); VB₂ (0.53-13.84 ng/mL); VB₃ (4.43-13.70 μg/mL); VB₆ (1.09-26.00 ng/mL); VB₉ (1.02-9.48 ng/mL); Mg (27.54-52.99 mg/L); Cu (0.57-1.64 mg/L); Fe (287.00-708.60 mg/L); Zn (4.10-20.85 mg/L); Se (70.57-167.63 μg/L). For females: VA (142.62-755.78 ng/mL); VD (10.10-66.20 ng/mL); VE (1.87-13.43 ng/mL); VB₁ (0.15-4.20 ng/mL); VB₂ (0.61-12.81 ng/mL); VB₃ (4.51-15.53 ng/mL); VB₆ (1.82-24.05 ng/mL); VB₉ (0.60-9.62 ng/mL); Mg (25.73-51.94 mg/L); Cu (0.58-1.41 mg/L); Fe (236.75-599.81 mg/L); Zn (3.93-22.22 mg/L); Se (70.57-167.63 μg/L).

Conclusions: This study presents venous whole blood micronutrient parameters that can facilitate nutrition-related epidemiological research and inform personalized intervention strategies, thereby enhancing the quality of healthcare for elderly individuals in China.

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用干血点技术建立中国健康老年人静脉全血微量营养素参考区间。

背景：静脉全血微量营养素参考区间（RIs）是评价人群营养状况的重要指标。然而，利用简单高效的干血点（DBS）技术建立微量营养素RIs的研究仍然很少。因此，本研究旨在通过DBS建立中国健康老年人静脉全血微量营养素的RIs。方法：从天津市宝坻区普通社区招募参与者。根据临床和实验室标准协会指南的建议、身体体征（体重指数和腰围）和血液生化参数（血脂状态和血红蛋白）确定健康人群。微量营养素水平通过DBS技术测定。对于正态分布的数据，RI计算为平均值 ± 2SD。对于非正态分布的数据，RI计算为第2.5至97.5个百分位数。结果：本研究确定了健康老年人8种维生素（维生素A、D、E、B1、B2、B3、B6、B9）和5种矿物质（Mg、Cu、Fe、Zn、Se）的RIs。男性的RIs值为：VA(157.76 ~ 828.81 ng/mL)；VD(8.89 - -58.32 ng / mL);VE(1.84 - -13.78 μg / mL); VB1 (0.19 -5.36 ng / mL); VB2 (0.53 -13.84 ng / mL);能(4.43 - -13.70 μg / mL);VB6(1.09 - -26.00 ng / mL); VB9 (1.02 -9.48 ng / mL);27.54 - -52.99毫克( Mg / L);铜(0.57 - -1.64 mg / L);铁(287.00 - -708.60 mg / L); 锌(4.10 -20.85 mg / L); Se(70.57 -167.63μg / L)。女性：VA(142.62-755.78 ng/mL)；VD(10.10 - -66.20 ng / mL);VE(1.87 - -13.43 ng / mL); VB1 (0.15 -4.20 ng / mL); VB2 (0.61 -12.81 ng / mL);能(4.51 - -15.53 ng / mL);VB6(1.82 - -24.05 ng / mL); VB9 (0.60 -9.62 ng / mL);25.73 - -51.94毫克( Mg / L);铜(0.58 - -1.41 mg / L);铁(236.75 - -599.81 mg / L); 锌(3.93 -22.22 mg / L); Se(70.57 -167.63μg / L)。结论：本研究提供了静脉全血微量营养素参数，可以促进营养相关的流行病学研究，为个性化干预策略提供信息，从而提高中国老年人的医疗保健质量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinica Chimica Acta 医学-医学实验技术

CiteScore

10.10

自引率

2.00%

发文量

1268

审稿时长

23 days

期刊介绍： The Official Journal of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) Clinica Chimica Acta is a high-quality journal which publishes original Research Communications in the field of clinical chemistry and laboratory medicine, defined as the diagnostic application of chemistry, biochemistry, immunochemistry, biochemical aspects of hematology, toxicology, and molecular biology to the study of human disease in body fluids and cells. The objective of the journal is to publish novel information leading to a better understanding of biological mechanisms of human diseases, their prevention, diagnosis, and patient management. Reports of an applied clinical character are also welcome. Papers concerned with normal metabolic processes or with constituents of normal cells or body fluids, such as reports of experimental or clinical studies in animals, are only considered when they are clearly and directly relevant to human disease. Evaluation of commercial products have a low priority for publication, unless they are novel or represent a technological breakthrough. Studies dealing with effects of drugs and natural products and studies dealing with the redox status in various diseases are not within the journal''s scope. Development and evaluation of novel analytical methodologies where applicable to diagnostic clinical chemistry and laboratory medicine, including point-of-care testing, and topics on laboratory management and informatics will also be considered. Studies focused on emerging diagnostic technologies and (big) data analysis procedures including digitalization, mobile Health, and artificial Intelligence applied to Laboratory Medicine are also of interest.