Journal of cannabis research最新文献

{"title":"Plant-parasitic nematodes on hemp in the Pacific Northwest of the United States.","authors":"Lester A Núñez-Rodríguez, Hannah M Rivedal, Cynthia M Ocamb, David H Gent, Inga A Zasada","doi":"10.1186/s42238-025-00301-y","DOIUrl":"10.1186/s42238-025-00301-y","url":null,"abstract":"<p><strong>Background: </strong>Plant-parasitic nematodes are one of the most important biotic factors that impact crop production globally. Since hemp cultivation in the U.S. was banned from 1970 to 2018, little information is available about current plant-parasitic nematode pressure on U.S. hemp production. The production of hemp has gained interest in Washington and Oregon, states where several genera of plant-parasitic nematodes have been associated with various crops. This report is the first to define plant-parasitic nematodes associated with hemp in these states in the Pacific Northwest.</p><p><strong>Methods: </strong>Soil and root samples from hemp fields were collected in early autumn in 2021, 2022, and 2023. The occurrence, population density, and identity of plant-parasitic nematodes in these samples were determined using morphological and molecular identification methods. A Bayesian analysis of available sequence data was used to analyze phylogenetic relationships of nematode species found in hemp fields. Additionally, the host status of hemp 'Alpha Explorer' to three plant-parasitic nematodes, Meloidogyne chitwoodi, M. hapla, and Pratylenchus neglectus, was tested under greenhouse conditions. The occurrence of plant-parasitic nematodes and reproduction factor (final population density/initial population density) values of the three nematode species were analyzed with non-parametric methods.</p><p><strong>Results: </strong>Pratylenchus spp. were the most frequent plant-parasitic nematodes recovered from soil samples, being present in ~ 63% of samples (n = 107). Only two endoparasitic nematodes, Meloidogyne spp. and Pratylenchus spp., were found in root samples, with Pratylenchus spp. as the most frequent (20 out of 24 fields). A large diversity of Pratylenchus spp. was detected in hemp root samples. Hemp 'Alpha Explorer' was a poor host for P. neglectus, resulting in low reproduction values (< 1). Additionally, results of our study indicated that hemp is not a host for M. chitwoodi.</p><p><strong>Conclusions: </strong>Pratylenchus spp. were the most frequent plant-parasitic nematodes found in hemp fields in Oregon and Washington. This study reports for the first time five Pratylenchus species (Pratylenchus crenatus, P. fallax, P. hexincisus, P. neglectus, and P. scribneri) associated with hemp in Oregon and Washington; P. penetrans was also found in the region on hemp. The host status results indicate that hemp can be considered a non-host for M. chitwoodi and a poor host for M. hapla and P. neglectus.</p>","PeriodicalId":101310,"journal":{"name":"Journal of cannabis research","volume":"7 1","pages":"47"},"PeriodicalIF":4.1,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144651777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patterns and factors among oncology fellows recommending medical cannabis to adults with cancer.","authors":"Kian Tehranchi, Rushad Patell, Poorva Bindal, Laura Dodge, Jason Freed, Mary Buss, Mara A Schonberg, Ilana Braun, Deepa Rangachari","doi":"10.1186/s42238-025-00293-9","DOIUrl":"10.1186/s42238-025-00293-9","url":null,"abstract":"<p><strong>Background: </strong>Medical cannabis consumption is rising, but limited evidence informs the safety and efficacy of cannabis use in cancer patients. A national survey of oncology trainees found that most fellows felt insufficiently informed to make clinical recommendations about cannabis.</p><p><strong>Aim: </strong>In this secondary analysis, we aimed to measure how frequently trainees recommend in favor of cannabis and determine factors influencing this clinical practice.</p><p><strong>Methods: </strong>In this cross-sectional survey study for fellows enrolled in oncology training programs across the United States, an online survey assessing trainee practices regarding medical cannabis was sent to 155 oncology fellowship program directors from January - March 2021; who were asked to distribute it to their fellows. The primary outcome was the frequency with which oncology fellows recommended cannabis in the prior year.</p><p><strong>Results: </strong>Nationally, 40 programs from 25 states participated, with 189 of 462 trainees across these programs responding (40.9% response rate). 22% (95% CI: 16.3-29.0%) of participants reported recommending medical cannabis to > 5 patients in the past year. 24% (95% CI: 18.4-30.5%) of participants had prior training in medical cannabis. Regarding participant characteristics, only prior training in medical cannabis was significantly associated with recommending cannabis to > 5 patients (RR: 2.4; 95% CI: 1.4-4.2).</p><p><strong>Conclusions: </strong>With increasing cannabis use among patients with cancer and given that a substantial number of oncology fellows recommend its use, it is crucial that fellowship training incorporate evidence-based curricula regarding medical cannabis use to guide informed decision-making between patients and their fellow providers.</p>","PeriodicalId":101310,"journal":{"name":"Journal of cannabis research","volume":"7 1","pages":"45"},"PeriodicalIF":4.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12261571/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144639184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of five cannabis oils with different CBD: THC ratios and terpenes on hypertension, dyslipidemia, hepatic steatosis, oxidative stress, and CB1 receptor in an experimental model.","authors":"Valentina Degrave, Michelle Berenice Vega Joubert, Camila Filippa, Paola Ingaramo, Lucía Torregiani, Yamile Soledad Caro, María Mercedes De Zan, María Eugenia D'Alessandro, María Eugenia Oliva","doi":"10.1186/s42238-025-00286-8","DOIUrl":"10.1186/s42238-025-00286-8","url":null,"abstract":"<p><strong>Background: </strong>Non-alcoholic fatty liver disease (NAFLD) is a common liver disorder caused by oxidative stress and dysregulation of lipid metabolism. The endocannabinoid system (ECS), particularly the type 1 cannabinoid (CB1) receptor, plays a crucial role in NAFLD progression. Cannabinoids, such as cannabidiol (CBD) and tetrahydrocannabinol (THC), along with terpenes, such as beta-myrcene and d-limonene, have shown potential therapeutic effects on liver health, particularly in reducing oxidative stress and modulating lipid metabolism. This study aimed to analyse the effects of five cannabis oils (COs), each with different CBD:THC ratios and terpenes content, on hypertension, dyslipidemia, hepatic steatosis, oxidative stress, and CB1 receptor expression in an experimental model of NAFLD induced by a sucrose-rich diet (SRD) in Wistar rats for 3 weeks.</p><p><strong>Methods: </strong>Male Wistar rats were fed either a: (1) reference diet (RD; standard commercial laboratory diet) or a: (2) sucrose-rich diet (SRD) for 3 weeks. 3 to 7 SRD + CO as following: (3) SRD + THC; (4) SRD + CBD; (5) SRD + CBD:THC 1:1; (6) SRD + CBD:THC 2:1; and (7) SRD + CBD:THC 3:1. The COs were administered orally at a dose of 1.5 mg total cannabinoids/kg body weight daily. The cannabinoid and terpenes content of all COs used in the study was determined. The terpenes found in COs were beta-myrcene, d-limonene, terpinolene, linalool, beta-caryophyllene, alpha-humulene, (-)-guaiol, (-)-alpha-bisabolol. During the experimental period, body weight, food intake and blood pressure were measured. Serum glucose, triglyceride, total cholesterol, uric acid, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (AP) levels were evaluated. Liver tissue histology, NAFLD activity score (NAS), triglyceride and cholesterol content, lipogenic enzyme activities, enzyme related to mitochondrial fatty acid oxidation, reactive oxygen species (ROS), thiobarbituric acid reactive substance (TBARS), and antioxidant enzyme activities were also evaluated. The CB1 receptor expression was also determined.</p><p><strong>Results: </strong>The results showed that SRD-fed rats developed hypertension, dyslipidemia, liver damage, hepatic steatosis, lipid peroxidation, and oxidative stress. This was accompanied by upregulation of liver CB1 receptor expression. CBD-rich CO, CBD:THC 1:1 ratio CO; CBD:THC 2:1 ratio CO and CBD:THC 3:1 ratio CO showed antihypertensive properties. THC-rich CO, CBD:THC 1:1 ratio CO; CBD:THC 2:1 ratio CO showed the greatest beneficial effects against hepatic steatosis and liver damage. All COs exhibited antioxidant effects in liver tissue. This was associated with normal liver CB1 receptor expression.</p><p><strong>Conclusions: </strong>This study demonstrated that COs, particularly THC-rich CO, CBD:THC ratio 1:1 CO, CBD:THC ratio 2:1 CO and terpenes, can effectively reduce dyslipidemia, liver damage and hepatic steatosis in SRD-in","PeriodicalId":101310,"journal":{"name":"Journal of cannabis research","volume":"7 1","pages":"46"},"PeriodicalIF":4.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12261622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144639183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patterns of medicinal cannabis prescriptions in diverse patient populations: a retrospective analysis.","authors":"Omer Edni, Eviatar Naamany, Shimon Izhakian, Shachaf Shiber","doi":"10.1186/s42238-025-00307-6","DOIUrl":"10.1186/s42238-025-00307-6","url":null,"abstract":"<p><strong>Introduction: </strong>The use of medicinal cannabis is increasing worldwide and has applications in managing a wide range of conditions, including neurological, rheumatological, and gastrointestinal diseases. Despite its growing prevalence, there are limited data on patterns of cannabis prescription across varying disease groups and demographic subgroups.</p><p><strong>Methods: </strong>This retrospective observational cohort study analysed cannabis usage patterns among 263 patients from the cannabis user clinic at Rabin Medical center (RMC), a tertiary hospital in Israel. To minimise the inclusion of recreational cannabis users, only patients aged 30 years and older were included. Patients were categorised into three groups based on their primary medical condition: neurological (n = 63), rheumatological (n = 106), and gastrointestinal (n = 94). Data collected included: demographic information, cannabis dosage, Tetrahydrocannabinol (THC) content, cultivated variety preference (sativa vs. Indica), and method of consumption (smoking vs. oil). Statistical analyses were conducted using ANOVA, Kruskal-Wallis, chi-square, and t-tests to compare cannabis prescription patterns between disease and demographic groups.</p><p><strong>Results: </strong>Significant differences in cannabis prescription patterns were observed across disease groups. Patients with gastrointestinal conditions were prescribed the highest mean monthly cannabis dose (22.26 ± 13.60 g), while those with neurological conditions had the highest oil consumption (31.75%). Sex-based differences were notable, with male patients being prescribed significantly higher doses of cannabis (25.48 ± 15.15 g) and higher THC content (14 ± 6.56%) compared to female patients (17.32 ± 9.93 g; THC: 11.39 ± 6.48%).</p><p><strong>Discussion: </strong>The study highlights variations in cannabis prescription patterns based on both medical conditions and demographic factors. Male patients received higher doses and THC-rich formulations, while patients with gastrointestinal conditions had the highest cannabis prescription overall. These findings suggest the need for individualised cannabis therapy based on patient characteristics and the specific condition being treated.</p><p><strong>Conclusion: </strong>Medicinal cannabis usage patterns vary significantly across disease and demographic groups. Personalised cannabis treatment plans, informed by both clinical and demographic factors, are essential to optimising patient outcomes. Further research is needed to develop more precise guidelines for prescribing medicinal cannabis.</p>","PeriodicalId":101310,"journal":{"name":"Journal of cannabis research","volume":"7 1","pages":"43"},"PeriodicalIF":4.1,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12255969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Medicinal and combined medicinal/recreational cannabis use in California following the passage of Proposition 64.","authors":"Daniel Ageze, Renee Dell'Acqua, Thomas D Marcotte, Jill Rybar, Sara Baird, Alice Gold, Tom Shaughnessy, Ilene Lanin-Kettering, Linda Hill","doi":"10.1186/s42238-025-00285-9","DOIUrl":"10.1186/s42238-025-00285-9","url":null,"abstract":"<p><strong>Background: </strong>Proposition 64, the Adult Use of Marijuana Act of 2016, reshaped cannabis use in California. This study explores the use patterns of people who use cannabis for medicinal-only and combined medicinal and recreational use after implementation of Proposition 64.</p><p><strong>Methods: </strong>A quantitative, population-based online questionnaire included 4,020 current cannabis users, 523 former users, and 635 non-users. This analysis focuses on participants who self-identified as using cannabis for medicinal-only (n = 711) or both medicinal and recreational (M + R, n = 1719) purposes.</p><p><strong>Results: </strong>Sixty one percent of current cannabis users report medicinal use. Medicinal-only users were more likely to be female (OR 1.6, p < 0.001), have kids in household (OR 1.5, p < 0.001), and began cannabis use later (mean age 34 vs. 23, p < 0.001). Pain relief was the predominant reason for use, followed by sleep, anxiety, and stress relief. While both groups reported positive effects, M + R users experienced more negative side effects. Fewer medicinal-only users cited a desire to \"feel the high,\" (42% vs. 75% M + R, p < 0.001). Medicinal-only users felt less comfortable discussing cannabis with primary care providers than M + R users (75% vs 83%, p < 0.01). All users were more likely to seek information online (44-57%) or from friends/family (47-52%) than health professionals (26-27%). Dispensaries were the main cannabis source for both medicinal groups (72% M vs. 84% M + R, p < 0.01), with licensure being very or extremely important (72% M, 66% M + R, p < 0.01). Monthly spending for medicinal-only users was lower ($127 vs. $186 for M + R, p < 0.001), and they were more likely than M + R users to wait before feeling safe to drive after using cannabis.</p><p><strong>Conclusion: </strong>People who use medicinal cannabis alone vary in key areas from people who use cannabis for both medicinal and recreational reasons. The need for better patient-provider relationships and clinically informed guidance is evident to support medicinal cannabis users.</p>","PeriodicalId":101310,"journal":{"name":"Journal of cannabis research","volume":"7 1","pages":"44"},"PeriodicalIF":4.1,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12255994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge and attitudes toward recreational cannabis legalization among California residents: a population-matched questionnaire about Proposition 64.","authors":"Daniel Ageze, Sara Baird, Thomas D Marcotte, Ahmed Elashmawy, Angela Wong, Renee Dell'Acqua, Jill Rybar, Sarah Hacker, Alice Gold, Tom Shaughnessy, Ilene Lanin-Kettering, Linda L Hill","doi":"10.1186/s42238-025-00304-9","DOIUrl":"10.1186/s42238-025-00304-9","url":null,"abstract":"<p><strong>Background: </strong>Implemented in 2018, Proposition 64: The Adult Use of Marijuana Act (Prop 64; passed in 2016) legalized adult recreational cannabis use in California. This analysis of the Impact 64 study aimed to assess knowledge about Prop 64 and attitudes toward cannabis legalization by California residents.</p><p><strong>Methods: </strong>A mixed qualitative and quantitative questionnaire about cannabis and Prop 64 was completed by 4,020 current cannabis users, 523 former users, and 635 non-users who were selected from an initial pool demographically matched to the 2020 California census. Quantitative questions about Prop 64 knowledge and attitudes were selected for this sub-analysis. Chi-squared tests and nominal logistic multivariate analysis were used to assess knowledge and attitudes based on demographic characteristics and cannabis use factors.</p><p><strong>Results: </strong>Current users had a mean age of 42 years and were 59% male, and more than 90% had heard of Prop 64. The 71% of current users who described themselves as somewhat or very familiar with Prop 64 were more likely to be employed full-time (AOR 1.5, p < 0.001), have no minors in the household (AOR 1.4, p < 0.001), and live in Central California (AOR 1.3, p < 0.001). Awareness of specific regulations of the associated laws was low among current users, including for possession (49%), transportation (41%), and gifting (30%) of cannabis, as well as for age and possession limits. Attitudes were mixed, though current users had more positive attitudes than former and non-users for almost all measures (p < 0.001). Only 30% of current cannabis users, believe cannabis should be legal to use in more public places, and only half of current users felt that Prop 64 made cannabis products and cannabis acquisition safer.</p><p><strong>Conclusions: </strong>Four years after implementation, knowledge gaps around Prop 64 are widespread. Effective messaging is needed to increase awareness and bridge knowledge gaps, which can ultimately minimize public harm.</p>","PeriodicalId":101310,"journal":{"name":"Journal of cannabis research","volume":"7 1","pages":"42"},"PeriodicalIF":4.1,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12254984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144621670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subsurface drip irrigation reduces weed infestation and irrigation water use while increasing inflorescence and cannabinoid yield in an outdoor tunnel Cannabis sativa L. production system.","authors":"Christian Büser, Jens Hartung, Simone Graeff-Hönninger","doi":"10.1186/s42238-025-00302-x","DOIUrl":"10.1186/s42238-025-00302-x","url":null,"abstract":"<p><p>Cannabis (Cannabis sativa L.) has served as a valuable medicinal plant for thousands of years and is experiencing a resurgence in cultivation and research due to recent legal changes. However, the resource-intensive nature of cannabis cultivation, particularly water and energy demands, poses significant environmental challenges. Outdoor cultivation in a semi-controlled environment can reduce those energy demands but necessitates irrigation. Drip irrigation (DI) is the most commonly used irrigation method but is often criticized for its susceptibility to water losses through evaporation and the risk of surface runoff. Subsurface Drip Irrigation (SDI) provides a sustainable solution by minimizing evaporation losses while maintaining or increasing yields, thereby enhancing water use efficiency. In this study, we compared the effects of DI and SDI on weed infestation, total water usage, inflorescence yield, and water use efficiency of three CBD-rich cannabis chemotype III genotypes (Kanada, Terra Italia, FED) in an outdoor foil tunnel cultivation system. SDI resulted in a reduction of irrigation water usage by 18.6% compared to DI. Remarkably, weed dry biomass was reduced by 93.2% in SDI. Concomitantly, inflorescence yield increased by 5% and CBD concentration by 9%. Overall, the water use efficiency of inflorescence yield and CBD concentration was significantly higher in SDI than in DI. Our results indicated that implementing SDI instead of DI can significantly decrease irrigation water use and reduce weed infestation while increasing inflorescence and CBD yield, thus reducing the environmental challenges associated with cannabis cultivation.</p>","PeriodicalId":101310,"journal":{"name":"Journal of cannabis research","volume":"7 1","pages":"41"},"PeriodicalIF":4.1,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12247431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling herbicide stress and its impact on metabolite profiling in Cannabis sativa: an investigative study.","authors":"Sabreen Bashir, Navneet Kaur, Agrataben Vadhel, Awadhesh Kumar Verma, Madhuri Girdhar, Tabarak Malik, Anil Kumar, Anand Mohan","doi":"10.1186/s42238-025-00300-z","DOIUrl":"10.1186/s42238-025-00300-z","url":null,"abstract":"<p><strong>Background: </strong>Cannabis sativa L., renowned for its versatility in pharmaceutical, textile, and cosmetic industries, is highly susceptible to several agronomic and environmental factors, particularly herbicides. These chemical agents, while commonly used for weed control, can adversely affect plant growth, physiology, and secondary metabolite production. Understanding the plant's response to such external stressors is essential for optimizing its cultivation and ensuring the quality of its bioactive compounds.</p><p><strong>Methods: </strong>In our current work, we studied the impact of two herbicides- glyphosate and metribuzin on the morpho-physiological and biochemical characteristics of cannabis plants. The secondary metabolite production analysis was carried out using Gas Chromatography-Mass S pectrometry (GC-MS). Furthermore, in silico studies using molecular modelling and optimization via Density Functional Theory (DFT) were performed, followed by molecular docking.</p><p><strong>Results: </strong>It was observed that both herbicides greatly impact overall plant productivity including primary and secondary metabolite production. Further, glyphosate treatment caused an increase in fatty acid synthesis while the contrary was observed in case of metribuzin. Also, herbicide stress leads to the synthesis of cannabidivarol and cannabidiol although they were absent in the untreated group. These findings provide crucial insights for optimizing agricultural practices in cannabis cultivation. Moreover, molecular simulation results showed that both metribuzin and glyphosate bind at the active pocket of Tetrahydrocannabinolic acid synthase (THCA synthase) and offer a mechanistic explanation for the observed variations in Δ9 -tetrahydocannabinol (THC) levels by suggesting that both herbicides inhibit THCA synthase activity, contributing to a deeper understanding of herbicide-plant interactions at the molecular level.</p><p><strong>Conclusions: </strong>Our findings indicate that herbicide stress impacts overall cannabis productivity and alters biosynthesis. The stress notably stimulates the production of cannabidivarol and cannabidiol. In addition, molecular docking studies revealed that metribuzin binds to the same active channel as Cannabigerolic acid (CBGA)- the THC precursor, while glyphosate binds at the entrance, thereby hindering THC production. This multifaceted approach guides sustainable farming strategies and has implications for manipulating cannabinoid profiles in pharmaceutical and other industrial applications.</p>","PeriodicalId":101310,"journal":{"name":"Journal of cannabis research","volume":"7 1","pages":"40"},"PeriodicalIF":4.1,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12236017/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Medical Cannabis Library: development of a curated database for research articles on cannabis therapeutic activity.","authors":"Dmitry Rodin, Yael Maizels, Igor Koman","doi":"10.1186/s42238-025-00295-7","DOIUrl":"10.1186/s42238-025-00295-7","url":null,"abstract":"<p><p>The Medical Cannabis Library (MCL) is a curated database designed to simplify the search for cannabis-related therapeutic research. Addressing the challenge of navigating vast and mixed-quality literature, the MCL consolidates over 11,000 relevant publications from Pubmed, focusing on cannabinoids as therapeutic agents. It features an advanced search interface allowing users to find information by diseases, conditions, symptoms, syndromes, and cannabinoids. The database also categorizes the relationship between cannabinoids and medical conditions as positive, negative, or neutral using natural language processing. This tool streamlines access to cannabis research, aiding professionals in understanding its therapeutic potential across various clinical indications. The MCL represents a significant advancement in organizing and accessing scientific knowledge in the field of medical cannabis.</p>","PeriodicalId":101310,"journal":{"name":"Journal of cannabis research","volume":"7 1","pages":"39"},"PeriodicalIF":4.1,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12236019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orally consumed cannabinoids: the effect of carrier oil on acute tissue distribution in male C57BL/6 mice.","authors":"Cody A C Lust, Lyn M Hillyer, Mitchell Pallister, Amanda J Wright, Michael A Rogers, Erin M Rock, Cheryl L Limebeer, Linda A Parker, David W L Ma","doi":"10.1186/s42238-025-00298-4","DOIUrl":"10.1186/s42238-025-00298-4","url":null,"abstract":"<p><strong>Background: </strong>Fundamental gaps in knowledge exist in understanding the tissue distribution of cannabinoids, cannabidiol (CBD) and tetrahydrocannabinol (THC), following oral ingestion. CBD and THC are lipid-soluble and oral bioavailability is increased when combined with long-chain fatty acid carrier oils prior to oral ingestion. Oils with eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) confer positive health benefits and have yet to be examined as a carrier oil for oral cannabinoid delivery thus, examination is warranted.</p><p><strong>Methods: </strong>This study investigated the acute tissue distribution of cannabinoids in serum, adipose, brain, liver, heart, and muscle of male C57BL/6 mice at 1, 2, and 3 h (H) post oral ingestion. Mice were gavaged with CBD (5 mg/kg) and THC (1 mg/kg) combined with either sesame (SES), mixed EPA/DHA, or DHA enriched (DHA) oil as a carrier. With assistance of the Analytical Facility for Bioactive Molecules (Toronto, Canada), tissue concentration of cannabinoids was quantified using liquid chromatography with tandem mass spectrometry.</p><p><strong>Results: </strong>SES oil resulted in a significantly greater concentration of CBD and THC (p < 0.05) across all tissues and times compared to the n-3 polyunsaturated fatty acid (PUFA) oils. The ratio of EPA:DHA in the carrier oils modestly affected distribution of cannabinoids to tissues, notably, DHA oil resulted in a greater concentration of CBD in the brain. Heart tissue had the highest concentration of CBD at 1 and 2H post-oral gavage, and adipose tissue had the highest concentration at 3H which was consistent across all three carrier oils.</p><p><strong>Conclusions: </strong>This study profiled the greatest number of tissues to-date for the acute distribution of CBD and THC following oral consumption with a lipid carrier in mice which demonstrated a non-uniform distribution to tissues over time. SES oil proved to be far more effective as a carrier oil at delivering orally consumed cannabinoids to tissues compared to two different n-3 PUFA containing oils. Further developing our fundamental understanding of cannabinoid distribution across tissues following their consumption from foods and pharmaceuticals are necessary to establish specific pharmacokinetic profiles to aid oral dosing strategies and maximize the bioactive potential of cannabinoids.</p>","PeriodicalId":101310,"journal":{"name":"Journal of cannabis research","volume":"7 1","pages":"38"},"PeriodicalIF":4.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12219598/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144546765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}