Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie最新文献

{"title":"Die Schutzwirkung von EDTA-Na-Extraktimpfstoffen aus schweinepathogenen E. coli-Bakterien im Feldversuch 2. Mitteilung: Immunisierung von Absatzferkeln","authors":"H. Mochmann ,&nbsp;J. Zwierzchowski ,&nbsp;Lisa Hering,&nbsp;J. Molenda,&nbsp;H.W. Ocklitz,&nbsp;M. Bocianowski,&nbsp;L. Austenat,&nbsp;W. Wałachowski,&nbsp;Z. Janas","doi":"10.1016/S0172-5599(80)80006-X","DOIUrl":"10.1016/S0172-5599(80)80006-X","url":null,"abstract":"<div><p>Pentavalent EDTA-Sodium-extract vaccines and monovalent EDTA-Sodium-extract vaccines obtained from swine-pathogenic <em>E. coli</em> were used to test their antiepizootic effect in industrial pig farms, 2929 piglets after weaning were included in this field trial, 1309 were immunized partly orally, partly i.m., 1620 piglets received placebos accordingly. By means of oral immunization the pentavalent vaccine was capable of reducing the morbidity of the vaccinated animals significantly. Intramuscular immunization with the pentavalent vaccines produced significant differences in morbidity too.</p><p>A monovalent vaccine obtained from <em>E. coli</em> O149 and <em>E. coli</em> O8 was shown to be equally effective, whereas a monovalent vaccine obtained from <em>E. coli</em> O115 was ineffective. But in this stock there were infections with O149 but none with O115 or O8.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"247 2","pages":"Pages 208-220"},"PeriodicalIF":0.0,"publicationDate":"1980-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80006-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83564433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolierung und Charakterisierung von erythrogenen Toxinen I. Untersuchung des von Streptococcus pyogenes, Stamm NY-5 gebildeten Toxins A","authors":"Dieter Gerlach ,&nbsp;Heide Knöll,&nbsp;Werner Köhler","doi":"10.1016/S0172-5599(80)80004-6","DOIUrl":"10.1016/S0172-5599(80)80004-6","url":null,"abstract":"<div><p>An erythrogenic toxin was isolated from culture supernatants of <em>Streptococcus pyogenes</em>, strain NY-5 (type 12) grown in yeast extract-pepton-dialysate medium. After concentration by evaporation, a crude material was obtained by precipitation with ethanol. This material was prepurified by ion exchange chromatography using a DEAE-Sepharose Cl-6B column. After precipitation of the active material with (NH₄)₂SO₄ the redissolved precipitate was dialyzed against acetate buffer and rechromatographed on a CM-Sepharose Cl-6B column. The toxin was obtained by stepwise elution with 0.02 M acetate buffer, pH 5.0, and 0.05 M phosphate buffer, pH 6.5, followed by a last purification step on a Sephacryl S-200 column.</p><p>The purified toxin behaved homogenously in SDS electrophoresis, the molecular weight being about 28000. Amino acid analysis showed only one cysteine residue per molecule. The molecule was insensitive to 2-mercaptoethanol and alkylation. Isoelectric focusing yielded two narrow bands having an isoelectric point of 5.2. The toxin showed a serological reaction of identity with an antiserum against streptococcal pyrogenic exotoxin A (SPE-A) but was shown to have a different molecular weight. The purified material (erythrogenic toxin A) was pyrogenic and toxic for rabbits as well as mitogenic for human lymphocytes. A positive skin reaction in guinea pigs could be obtained by as little as 5 × 10⁻⁸ mg.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"247 2","pages":"Pages 177-191"},"PeriodicalIF":0.0,"publicationDate":"1980-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80004-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83762561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative Bestimmung der Zerstörung von Nervenzellen im Cortex nach Virusencephalitis (17 D-Gelbfieber), eine Grundlage zur Beurteilung pathologischer Prozesse im ZNS","authors":"C. Museteanu ,&nbsp;Jutta Haase,&nbsp;R. Stiens,&nbsp;G. Henneberg","doi":"10.1016/S0172-5599(80)80001-0","DOIUrl":"10.1016/S0172-5599(80)80001-0","url":null,"abstract":"<div><p>A loss of nerve cells in the cortex after encephalitis was reported already in the classical work by <em>Nissl, Spielmeyer</em>, and <em>Spatz</em>. A loss of nerve cells will become only noticeable if it amounts to at least 50%. But as such clear pictures are rarely found, estimations were always considered as doubtful and incorrect.</p><p>Not only the number of cells is important in consideration of the morphological change in the cortex but also the size of cells. The development of a new apparatus made it possible to consider two structural parameters: the surface and the perimeter of cells.</p><p>In 497 histological serial preparations obtained from 43 mouse brains we determined the number, the surface, and the perimeter of nerve cells. 39 animals were infected intracerebraly with yellow fever 17 D; 4 normal animals served as controls. Among the infected animals, 8 were treated with a mucopolysaccharide.</p><p>The cells were counted within a determined area (standard unity); this area was taken from the angle between the curbura exterior and the sulcus anterior-posterior of the brain.</p><p>There was a significant difference between the number of nerve cells in normal (278) and in infected (202) animals. The animals treated with mucopolysaccharide showed a normal quantity of nerve cells but surface and perimeter corresponded to the data of the infected ones. The surface of normal animals was at 23.39, that of infected at 14.29. There was also a significant difference with regard to the cell perimeter: normal 14.97, infected 12.02. This means a shrinking of cells. The cell shrinkage revealed that the nerve cells were affected.</p><p>The measurement of these three parameters presents new and exact statistical findings which enable a reconsideration of neurovirulence.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"247 2","pages":"Pages 143-163"},"PeriodicalIF":0.0,"publicationDate":"1980-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80001-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85382286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Die Sekretion von Mediatoren der Entzündung und der antimikrobiellen Abwehr durch Makrophagen","authors":"Volker Brade","doi":"10.1016/S0172-5599(80)80011-3","DOIUrl":"10.1016/S0172-5599(80)80011-3","url":null,"abstract":"<div><p>Macrophages are highly differentiated mononuclear phagocytes which originate from stem cells of the bone marrow. The secretory potential of these cells has been recognized in recent years. Major secretory products comprise lysosomal enzymes, complement proteins, prostaglandins and interferon. Secretion of lysosomal hydrolases and proteinases is most prominent in macrophages stimulated <em>in vivo</em> or <em>in vitro</em> (Fig. 4). Lysosomal enzyme secretion may be an important factor in the induction and maintenance of inflammatory reactions. Complement (C) proteins secreted by macrophages belong to the classical activation unit (C1, C4 and C2), alternative activation unit (C3, B, D, P) and to the group of delayed-acting C proteins (Fig. 7). Therefore macrophages produce at local sites the C component C3 from which biologically active C3 fragments (C3a, C3b, C3e) can be generated. These C3 fragments mediate inflammatory and cytotoxic reactions and also promote phagocytic processes (Fig. 6). Cleavage of secreted C3 into the active fragments may occur by enzymes derived from both C activation units or by secreted lysosomal proteinases (Fig. 8). Stimulated macrophages also synthesize and release prostaglandins. These compounds which have inflammatory as well as antiinflammatory effects (Fig. 12) may play an important regulatory role in inflammatory processes. Interferon has been also recognized as a secretory product of macrophages. This substance supports antimicrobial resistance by its phagocytosis-increasing effect and its antiviral activity.</p><p>The secretory function of macrophages as well as the biological effects of secreted mediators are highly susceptible to modulation. Thus, C3 fragments stimulate the secretion of lysosomal enzymes (Fig. 6) whereas prostaglandins inhibit their release (Fig. 12). The inflammatory reactions induced by lysosomal enzymes may be further increased by the generation of C3b which stimulates additional lysosomal enzyme release (Fig. 4). These and other examples suggest that endogenous control mechanisms may have a strong influence on the secretory function of macrophages as well as on the biological activity of secreted mediators.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"247 2","pages":"Pages 259-275"},"PeriodicalIF":0.0,"publicationDate":"1980-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80011-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79887435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial Action of Quinolones on Escherichia coli IIa. Conditions Required for Lethality: Effect of Anaerobiosis, Inoculum Size and Media Composition","authors":"Fakhira Mahmood ,&nbsp;Noorul Hasan Mahmood","doi":"10.1016/S0172-5599(80)80007-1","DOIUrl":"10.1016/S0172-5599(80)80007-1","url":null,"abstract":"<div><p>Quinolones with carboxylic or nitroside groups behaved differently <em>in vitro</em> under various physiological conditions tested. CarboxyQ was not active during the lag phase of <em>E. coli</em> and its activity was proportional to the growth rate under anaerobic conditions. NitroQ was immediately active on the cells soon after the inoculum was introduced under similar conditions. High cell density did not change the typical behaviour of these drugs. Only nitroQ was active in nitrogen-free medium while both were inactive in glucose-free medium. Chloramphenicol, reduced the activity of carboxyQ while that of nitroQ was unaffected.</p></div><div><p>Unter verschiedenen geprüften physiologischen Bedingungen zeigten Chinolone mit Carboxyl- oder Nitrosidgruppen ein unterschiedliches Verhalten in vitro. Carboxychinolon war während der lag-Phase von <em>E. coli</em> nicht aktiv, und seine Aktivität war der Wachstumsgeschwindigkeit unter anaeroben Bedingungen proportional. Nitrochinolon zeigte eine unmittelbare Aktivität gegenüber den Zellen kurz nach Zugabe des Inoculums unter ähnlichen Bedingungen. Eine hohe Zelldichte veränderte das typische Verhalten dieser Arzneimittel nicht. Im stickstofffreien Medium war nur Nitrochinolon aktiv, während im glukosefreien Medium beide inaktiv blieben. Chloramphenicol minderte die Aktivität von Carboxychinolon, beeinflußte aber die Aktivität von Nitrochinolon nicht.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"247 2","pages":"Pages 221-228"},"PeriodicalIF":0.0,"publicationDate":"1980-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80007-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88189140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Messung von α2-Makroglobulin in Humanseren durch quantitative Hemmung einer sauren Staphylokokken-Protease","authors":"Ursula Neubüser ,&nbsp;Jörg Brückler ,&nbsp;Hans Blobel","doi":"10.1016/S0172-5599(80)80002-2","DOIUrl":"10.1016/S0172-5599(80)80002-2","url":null,"abstract":"<div><p>Concentrations of α₂-macroglobulin could be determined in the sera of 215 blood donors and 94 patients with various internal diseases by quantitative inhibition of an acid protease from <em>Staphylococcus aureus</em>, M 135 (fig. 1, 2). The determinations agreed closely with those obtained by immunodiffusion (tab. 1, fig. 3). However, the α₂-macroglobulin-measurements by the protease method required only 4 h and 50 μl serum. This procedure would also be suitable for the determination of α₂-macroglobulin in sera from experimental and domestic animals.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"247 2","pages":"Pages 164-169"},"PeriodicalIF":0.0,"publicationDate":"1980-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80002-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84552282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein-A-Hämagglutinationstest, eine zuverlässige Methode zur schnellen Identifizierung von S. aureus","authors":"Ludwig Essers ,&nbsp;Klaus Radebold","doi":"10.1016/S0172-5599(80)80003-4","DOIUrl":"10.1016/S0172-5599(80)80003-4","url":null,"abstract":"<div><p>369 staphylococcal strains isolated from clinical material were examined for tubeagglutination with sensitized sheep red cells in a standarized assay to study its reliability for routine identification of <em>S. aureus</em>. Colonies isolated from blood agar plates correlated in 99.5% with the (optimized) coagulase reaction. The test is easily to perform and results can be read after 2 hours, whereas the reference methods coagulase, hyaluronidase and deoxyribonuclease took as much as 24 h. The reliability of these tests is discussed.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"247 2","pages":"Pages 170-176"},"PeriodicalIF":0.0,"publicationDate":"1980-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80003-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84095522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Die Schutzwirkung von EDTA-Na-Extraktimpfstoffen aus schweinepathogenen E. coli-Bakterien in Feldversuchen 1. Mitteilung: Direkte und indirekte Immunisierung von Saugferkeln","authors":"H. Mochmann ,&nbsp;J. Zwierzchowski ,&nbsp;Lisa Hering,&nbsp;J. Molenda,&nbsp;H.W. Ocklitz,&nbsp;M. Bocianowski,&nbsp;L. Austenat,&nbsp;W. Wałachowski,&nbsp;Z. Janas","doi":"10.1016/S0172-5599(80)80005-8","DOIUrl":"10.1016/S0172-5599(80)80005-8","url":null,"abstract":"<div><p>Pentavalent EDTA-Sodium-extract-vaccines and monovalent EDTA-Sodium-extract-vaccines obtained from swine-pathogenic <em>E. coli</em> and produced in Berlin-Buch were used in field trials to test their antiepizootic effect in industrial pig-farms in the district of Wrocław. The pentavalent vaccine contained extracts from <em>E. coli</em> O138, O139, O141, O147, and O149, whereas extracts from O8, O115 and O149 were used as monovalent vaccines.</p><p>The field trials were conducted as blind trials in closed stocks of pigs. We employed direct oral immunization of suckling piglets and indirect immunization via sow vaccination. Direct oral immunization was tested in 382 suckling piglets. 190 animals were vaccinated, 192 animals received placebo.</p><p>Neither the pentavalent vaccine nor a monovalent vaccine obtained from an epizootie strain of this stock gave protection in terms of a decrease in the duration of disease or a reduction of morbidity and mortality. Direct oral-immunization of suckling piglets thus was ineffective.</p><p>Indirect immunization of the suckling piglets via vaccination of sow was performed in 189 pregnant sows 18–15 days before farrowing. 84 sows received vaccines i.m., 105 were given placebo i.m. These sows farrowed 1717 pigs, 777 of which were immunized indirectly and 940 treated with placebos. With the pentavalent vaccine and with the monovalent vaccine obtained from an epizootic strain we achieved significant protection of the indirect vaccinated suckling piglets.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"247 2","pages":"Pages 192-207"},"PeriodicalIF":0.0,"publicationDate":"1980-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80005-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82136219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution of Pseudomonas aeruginosa Serotypes in Bacteriologic Specimens and their Aminoglycoside Resistance","authors":"U. Ullmann ,&nbsp;R.-M. Schmülling","doi":"10.1016/S0172-5599(80)80009-5","DOIUrl":"10.1016/S0172-5599(80)80009-5","url":null,"abstract":"<div><p>With regard to that of other facultatively pathogenic microorganisms, the rate of isolation of <em>Pseudomonas aeruginosa</em> was 12.5%. Most frequently the pathogen was isolated from wound swabs, tracheal secretion, throat swabs and urine. In 2.8% of the cases it was identified as the causative pathogen of sepsis. The serotype most commonly identified was the O antigen type 6, followed by types 11 and 1. The distribution of the serotypes observed in specimens and hospitals varied altogether. Epidemiologically striking was the incidence of serotype 1 in the urological division, of type 11 in the gynecological division, of type 6 in the pediatric division, and of type 4 in the dermatological division. Between 4 to 5% of the 618 strains examined exhibited resistance to gentamicin, tobramycin and sisomicin. Insensitivity to amikacin has not been observed. There were no statistically significant differences in incidence of resistance or the pattern of resistance as far as individual serotypes or isolates from the above mentioned divisions are concerned.</p></div><div><p>Die Isolierungsrate von <em>Pseudomonas aeruginosa</em> betrug 12,5% bezogen auf diejenige anderer fakultativ pathogener Mikroorganismen. Am häufigsten wurde er aus Wundabstrichen, Trachealsekret, Rachenabstrichen und Urin isoliert. Als Sepsiserreger trat er in 2,8% hervor. Der am meisten nachgewiesene Serotyp war der O Antigentyp 6, gefolgt von 11 und 1. Die Verteilung der Serotypen in den Untersuchungsmaterialien und Kliniken war durchweg unterschiedlich. Epidemiologisch auffallend war die Häufung von Serotyp 1 in der urologischen Klinik, die von 11 in der Frauen-, die von 6 in der Kinder- und die von 4 in der Hautklinik. Zwischen 4 bis 5% der 618 untersuchten Stämme waren gegenüber Gentamicin, Tobramycin und Sisomicin résistent. Unempfindlichkeit gegenüber Amikazin wurde nicht beobachtet. Statistisch signifikante Unterschiede in der Resistenzhäufigkeit oder im Resistenzmuster konnten weder für einzelne Serotypen noch für die Isolate aus den angeführten Kliniken nachgewiesen werden.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"247 2","pages":"Pages 241-247"},"PeriodicalIF":0.0,"publicationDate":"1980-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80009-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82864502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vergleichende Untersuchungen zur Bestimmung von Doxycyclin-Konzentrationen in biologischen Proben mit Hilfe der Hochdruck-Flüssigchromatographie und Agar-Diffusionsmethode","authors":"Ronald Böcker,&nbsp;Albert Weber","doi":"10.1016/S0172-5599(80)80012-5","DOIUrl":"10.1016/S0172-5599(80)80012-5","url":null,"abstract":"<div><p>A new high performance liquid chromatographic method for quantitative analysis of doxycycline has been compared with the microbiological assay. Both analytical methods yielded the same results of nearly 100% recovery when doxycycline was incubated with serum, lung-, or liver-homogenate (Table 1).</p><p>The advantages of the chromatographic method over the microbiological method are i. short and simple way of analysis (Fig. 1), ii. need of only small amounts of biological material.</p><p>In a pilot study doxycycline — after i.v. application to mice — could be analyzed in good agreement by both methods (Fig. 2). The microbiological analysis yielded in slightly lower serum- or organ-levels what might be due to an inactivation of the antibiotic attached to proteins to some extend.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"247 2","pages":"Pages 276-281"},"PeriodicalIF":0.0,"publicationDate":"1980-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80012-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84094413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}