Messung von α2-Makroglobulin in Humanseren durch quantitative Hemmung einer sauren Staphylokokken-Protease

Concentrations of α₂-macroglobulin could be determined in the sera of 215 blood donors and 94 patients with various internal diseases by quantitative inhibition of an acid protease from Staphylococcus aureus, M 135 (fig. 1, 2). The determinations agreed closely with those obtained by immunodiffusion (tab. 1, fig. 3). However, the α₂-macroglobulin-measurements by the protease method required only 4 h and 50 μl serum. This procedure would also be suitable for the determination of α₂-macroglobulin in sera from experimental and domestic animals.