Water Biology and Security最新文献

{"title":"The LxIS motif is the language between virus and host RLRs: From fish to mammalian viruses","authors":"Shun Li ,&nbsp;Long-Feng Lu ,&nbsp;Can Zhang","doi":"10.1016/j.watbs.2024.100246","DOIUrl":"10.1016/j.watbs.2024.100246","url":null,"abstract":"<div><p>Regulation of host interferon (IFN) production is a crucial strategy for viral immune escape. We propose that the LxIS motif language, a marker for exogenous viral proteins, interacts with host retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) factors. In this study, the viral proteins containing the LxIS motif of grass carp reovirus (GCRV), and cyprinid herpesvirus 3 (CyHV-3), which belong to the fish RNA and DNA viruses, were found to interact with RLRs, and the general functions of these proteins were negative regulation of IFN activation. Terrestrial animal viruses with this motif language, such as SARS-CoV-2 and so on, are also examined. Several viral proteins that were not reported previously were found to be associated with the RLR pathway. Taken together, the LxIS motif language elucidates a straightforward and efficient method for identifying viral proteins that target host RLRs to regulate IFN expression, and is conserved in both lower and higher vertebrates.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 2","pages":"Article 100246"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000027/pdfft?md5=d6551696a0359e530a152bc96e21506b&pid=1-s2.0-S2772735124000027-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139539838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel cell line established from skin tissue of gibel carp (Carassius gibelio) is susceptible to Carassius auratus herpesvirus infection","authors":"Qi-Qi Jiang ,&nbsp;Zhi Li ,&nbsp;Qi-Ya Zhang ,&nbsp;Fei Ke","doi":"10.1016/j.watbs.2024.100253","DOIUrl":"10.1016/j.watbs.2024.100253","url":null,"abstract":"<div><p>Frequent outbreaks of emerging infectious diseases in fish, such as <em>Carassius auratus</em> herpesvirus (CaHV) infection has caused great economic losses in China. However, the lack of a sensitive cell culture system has limited studies of CaHV. In the present study, a new cell line (gibel carp skin cell, GiCS) derived from gibel carp (<em>Carassius gibelio</em>) skin tissue was established to create a valuable tool for research of the virus. The GiCS cells consisted mainly of epithelial-like cells, which grew well at 25 ​°C in L-15 medium supplemented with 10–20% fetal bovine serum. Chromosomal analysis revealed that the skin cell line remained amphitriploid, with most chromosome counts being 156 (54%). The GiCS cells can be efficiently transfected and expressed exogenous genes. In particular, the GiCS cells showed high susceptibility to CaHV infection, which was confirmed by virus infection tests, detection of viral gene expression, and ultrastructural observation. To our knowledge, it is the first cell line that is highly permissive to CaHV infection. In addition, the cells also showed susceptibility to several aquatic animal viruses from different families including <em>Iridoviridae</em>, <em>Rhabdoviridae</em>, and <em>Reoviridae</em>. In conclusion, these results indicated that the establishment of the GiCS cell line is a significant advance that will be beneficial to future studies of CaHV and other aquatic animal viruses.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 2","pages":"Article 100253"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S277273512400009X/pdfft?md5=6973549fed436f9a79fdc1cb190f8efc&pid=1-s2.0-S277273512400009X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140768036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of stmn1a and genetic effects of a 22-bp Indel in its promoter region of bighead carp Hypophthalmichthys nobilis","authors":"Xiaoyu Zhou ,&nbsp;Yanhong Wu ,&nbsp;Junru Wang ,&nbsp;Xiaomu Yu ,&nbsp;Jingou Tong","doi":"10.1016/j.watbs.2024.100254","DOIUrl":"10.1016/j.watbs.2024.100254","url":null,"abstract":"<div><p>Stathmin 1 (<em>stmn1</em>) gene, which is involved in the control of cell cycle progression, cell proliferation, differentiation and motility, is a crucial relay in the signal transduction of multiple signaling pathways. Reports on the function of <em>stmn1</em> in fish are scarce. In this study, the characterization and function of <em>stmn1a</em> gene were explored in an important food fish, namely bighead carp (<em>Hypophthalmichthys nobilis</em>). The bighead carp <em>stmn1a</em> (<em>Hynstmn1a</em>) genomic sequence, which has 2995 bp with an ORF of 447 bp, is evolutionarily conserved in teleost fish. The highest level of expression of <em>Hynstmn1a</em> is in the un-fertilized eggs and spleen of adult bighead carp. The overexpression of <em>Hynstmn1a</em> facilitates cell cycle progression and suppresses apoptosis in EPC cells. In an siRNA assay, disruption of <em>stmn1a</em> in EPC cells resulted in the inhibition of cell cycle progression and promotion of apoptosis. Analysis of promoter activity indicated that an upstream transcription factor (foxo4) regulates <em>Hynstmn1a</em>. Zebrafish mutants with <em>stmn1a</em> knockout by CRISPR/Cas9 exhibited a significant retardation of body length (<em>p</em> ​&lt; ​0.05). There was no significant difference in body weight between <em>stmn1a</em>-mutant zebrafish and wild-type (<em>p</em> ​= ​0.053). A 22-bp Indel in the promoter region of <em>Hynstmn1a</em> with an observed heterozygosity (Ho) of 0.136 in a bighead carp population (<em>n</em> ​= ​191). The polymorphism of this 22-bp Indel genotype is significantly associated with body length (BL), head length (HL) (<em>p</em> ​&lt; ​0.05), body weight (BW), head height (HH), and head width (HW) (<em>p</em> ​&lt; ​0.01), showing its genetic effects on growth promotion. This study sheds light on the function of <em>stmn1a</em> in fish and demonstrates the potential of the 22-bp Indel of <em>Hynstmn1a</em> as a gene marker when selecting breeding individuals for faster growth and a bigger head of bighead carp in aquaculture.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 2","pages":"Article 100254"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000106/pdfft?md5=3254326309d5ea5e38dc120f43ee62ad&pid=1-s2.0-S2772735124000106-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140768134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zebrafish usp3 loss promotes hypoxic tolerance by disrupting deubiquitination of K63-polyubiquitinated hif-1αa","authors":"Jun Li ,&nbsp;Ziwen Zhou ,&nbsp;Xiaolian Cai ,&nbsp;Yanan Song ,&nbsp;Zhi Li ,&nbsp;Ziyi Li ,&nbsp;Wuhan Xiao ,&nbsp;Jing Wang","doi":"10.1016/j.watbs.2024.100245","DOIUrl":"10.1016/j.watbs.2024.100245","url":null,"abstract":"<div><p>The hypoxia-inducible factor (HIF)-mediated hypoxia signaling pathway influences oxygen homeostasis in aerobic organisms. This pathway is regulated by several other pathways. Elucidation of its regulation and the underlying mechanisms may provide insights into the strategies of adaptation and tolerance of organisms to hypoxia. In this study, we found that loss of ubiquitin-specific protease 3 (usp3) in zebrafish promotes hypoxia tolerance. Zebrafish usp3 specifically binds to hif-1αa and induces its proteasomal degradation, which is dependent on its deubiquitinase activity. This process leads to the suppression of hypoxia signaling under hypoxia. In addition, usp3 catalyzes the deubiquitination of K63-polyubiquitinated hif-1αa. Endogenous evidence indicated that mammalian USP3 behaves like zebrafish usp3 in regulating the activity of HIF-1α. These findings revealed a novel role for usp3 in influencing hypoxia signaling and showed that usp3-mediated HIF-1α degradation impairs hypoxia signaling, leading to a decrease in hypoxia tolerance.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 2","pages":"Article 100245"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000015/pdfft?md5=348303f64a87d878fced2e2fbb49bee5&pid=1-s2.0-S2772735124000015-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139393731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Best paper awards 2023","authors":"","doi":"10.1016/j.watbs.2024.100263","DOIUrl":"https://doi.org/10.1016/j.watbs.2024.100263","url":null,"abstract":"","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 2","pages":"Article 100263"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000192/pdfft?md5=606fa63046089cb75be12f2edacac4e4&pid=1-s2.0-S2772735124000192-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140820099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acknowledgement of reviewers who reviewed for Water Biology and Security in 2023","authors":"","doi":"10.1016/j.watbs.2024.100264","DOIUrl":"https://doi.org/10.1016/j.watbs.2024.100264","url":null,"abstract":"","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 2","pages":"Article 100264"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000209/pdfft?md5=1969321f678fb3971c089e562aa976d6&pid=1-s2.0-S2772735124000209-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140820100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-species probiotics enhance survival, growth, intestinal microbiota and disease resistance of rohu (Labeo rohita) larvae","authors":"Zannatul Ferdous ,&nbsp;Md Kabir Hossain ,&nbsp;Md Hadiuzzaman ,&nbsp;S.M. Rafiquzzaman ,&nbsp;KM Abdul Halim ,&nbsp;Tanvir Rahman ,&nbsp;Md Ali Reza Faruk ,&nbsp;Zulhisyam Abdul Kari ,&nbsp;Md Shahjahan","doi":"10.1016/j.watbs.2023.100234","DOIUrl":"10.1016/j.watbs.2023.100234","url":null,"abstract":"<div><p>Multi-strain probiotics provide the most dependable approaches to improve health, immune response, and disease resistance in farmed fishes. In the present study, we examined the effects of multi-species probiotics on the survival, growth, immune response, and disease resistance of rohu (<em>Labeo rohita</em>) larvae. Newly hatched larvae from the day of first feeding (average weight of 0.003 ​g) were reared with multi-species probiotics having a combination of <em>Bacillus subtilis</em> (10⁹ colony forming units (cfu)/mL) and <em>Lactobacillus</em> spp. (<em>Lactobacillus plantarum, Lactobacillus buchneri -</em> 10¹¹ ​cfu/mL) in water containing doses of 0 (control-C), 0.5 ​mL/L (treatment 1-T1), and 1.0 ​mL/L (treatment 2-T2) in triplicates for 90 days. After the experiment, a challenge test was performed to assess the fish's resistance to pathogenic <em>Aeromonas veronii</em>. Significantly higher survival was recorded in larvae of treated groups (87% in T2 and 79% in T1) compared to the control (62%). Significantly higher growth performance (weight gain and specific growth rate—SGR) was shown by the probiotic-treated larval groups compared to the control. Probiotic supplementation resulted in significantly higher counts of total viable colony (TVC) and lactic acid bacteria (LAB) in the intestine. Some immunological parameters (mucosal fold fattening, goblet cell abundance, expansion of lamina propria and enterocytes) of the gut were significantly better in probiotic-treated fish. The liver of treated fish showed irregular shape nuclei turning into regular shape and reducing spaces between the hepatic cells. Probiotic-treated fish had the highest post-challenge survival rate (90%) against <em>A. veronii</em> infection. The erythrocytes of challenged fish treated with probiotics had significantly lower frequencies of various nuclear and cellular abnormalities. These findings suggest that multi-species probiotic supplements could improve the survival, growth, health status, and immune response of rohu in the early stages of its development.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 1","pages":"Article 100234"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735123001294/pdfft?md5=e67d96a3c9e25c5c59cc378525516569&pid=1-s2.0-S2772735123001294-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138615286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of environmentally relevant concentrations of micro(nano)plastics on aquatic microorganisms: Changes in potential function but not in overall composition","authors":"Zhenlu Wang ,&nbsp;Xianghong Dong ,&nbsp;Muzi Zhang ,&nbsp;Lei Gan ,&nbsp;Jian Shao ,&nbsp;Weiling Sun","doi":"10.1016/j.watbs.2023.100233","DOIUrl":"10.1016/j.watbs.2023.100233","url":null,"abstract":"<div><p>Micro(nano)plastics (MNPs) are a growing problem as persistent environmental pollutants. Here, we investigated the impact of MNPs on microorganisms in aquatic microbial floc exposed to NPs (80 ​nm) and MPs (8 ​μm) for 35 days. Water quality indicators were tested weekly and microbiological analyses were conducted on Day 7 and 28 after exposure. The results showed that there were significant differences in the levels of total ammonia nitrogen or nitrite between the MNPs groups and the control group, spanning from Day 7 to Day 28. For the microbial response, microbial community richness in the NPs and MPs groups were significantly increased at Day 7. Functional prediction showed that the relative abundances of bacteria associated with the “Forms Biofilms”, “Potentially Pathogenic”, “Plastic Degradation” and nitrogen cycle processes were significantly different after MNPs exposure. The results suggest that MNPs had no significant effect on the microbial diversity of mature microbial flocs. Findings suggest MPs could cause an increase in the relative abundance of potentially pathogenic bacteria, while NPs do not. In addition, stress associated with MNPs affected the nitrogen cycle of microorganisms, and NPs exerted greater impacts than MPs. Findings from this study further our understanding of the impact of MNPs at environmentally relevant concentrations on microorganisms in aquatic ecosystems.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 1","pages":"Article 100233"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735123001282/pdfft?md5=9d34fcfde166ef0ad957f845ebc61155&pid=1-s2.0-S2772735123001282-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138612489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bloom-forming cyanobacteria and dinoflagellates in five Argentinian reservoirs: Multi-year sampling","authors":"Florencia Soledad Alvarez Dalinger ,&nbsp;Borja Claudia Nidia ,&nbsp;Verónica Laura Lozano ,&nbsp;Liliana Beatriz Moraña ,&nbsp;María Mónica Salusso","doi":"10.1016/j.watbs.2023.100232","DOIUrl":"https://doi.org/10.1016/j.watbs.2023.100232","url":null,"abstract":"<div><p>Harmful algal blooms are important threats to reservoir condition. Over a 15-year period, we sampled five Argentinian reservoirs to identify the responsible species for harmful algal blooms and determine the water quality factors driving their occurrence. These reservoirs exhibit diverse morphological and hydrological characteristics and are distributed across varying climatic zones. Cyanobacterial blooms included an array of species, including toxin producers such as <em>Raphidiopsis raciborskii, Aphanizomenon gracile</em>, and <em>Microcystis aeruginosa</em>. In contrast, dinoflagellate blooms were predominantly characterized by <em>Ceratium</em>. The cyanobacterial blooms primarily occurred in the shallower reservoirs located in warmer regions, whereas dinoflagellate blooms occurred where temperatures were lower. The most intense blooms occurred during the summer, and although cyanobacterial and chlorophyte blooms co-occurred, they never coexisted with dinoflagellate blooms. We identified cyanotoxins in the reservoirs over the past three years, a phenomenon previously unreported in the region. Given that these reservoirs are drinking water sources for human populations, ongoing and systematic monitoring is needed to protect public health. Controlling the proliferation of algae and cyanobacteria in reservoirs requires resource management at the watershed level, and in the case of the Itiyuro and El Limón, requires binational management with Bolivia.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 1","pages":"Article 100232"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735123001270/pdfft?md5=0407745454e31815798aa73ba5d8733c&pid=1-s2.0-S2772735123001270-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139419385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-ecological remediation of freshwater aquaculture environments: A systematic review and bibliometric analysis","authors":"Qiaohong Zhou ,&nbsp;Kaixuan Wu ,&nbsp;Lu Yao ,&nbsp;Ruonan Chen ,&nbsp;Shouzhuang Liu ,&nbsp;Hao Xing ,&nbsp;Linmei Nie ,&nbsp;Zhenbin Wu","doi":"10.1016/j.watbs.2023.100229","DOIUrl":"10.1016/j.watbs.2023.100229","url":null,"abstract":"<div><p>Freshwater aquaculture plays an important role in the global aquaculture industry. The success of freshwater aquaculture relies on the integration of bio-ecological remediation technologies to ensure its sustainable development. This paper systematically reviews the global literatures on bio-ecological remediation of freshwater aquaculture environments from 1990 to 2021, and discusses in detail the research progress and status quo in this field using both bibliometric analysis and content analysis. Our results indicate that the number of publications per year has increased year by year, with a particularly rapid increase after 2015. Institutions and researchers from China and the USA publish the greatest number of papers and contribute the most to this field. By analyzing the research progress in this field, we also find that the protection and restoration of freshwater aquaculture environments has received attention since as early as 1990, such as the application of constructed wetlands and recirculating aquaculture systems. Recently, researchers have increasingly focused on the use of microbial communities in remediation. Furthermore, vanRijn, J's papers are of great value to the research in this field. In content analysis, the application of primary technologies, including plant selection, aquatic animals, microorganisms, and biological filtration and purification equipment, as well as ecological engineering technologies with combinations of various types of organisms, is described to improve the effectiveness and efficiency of remediation. The performance, advantages, disadvantages, or limitations and prospects of these technology applications are analyzed in detail. By comparison, ecological engineering is shown to be the best remediation technology, being most comprehensive, and well-established. Taken together, this paper composes and summarizes existing work in this field, and provides guidance for future research on the improvement of existing technologies and the development of new technologies, with the aim of enhancing remediation capability, improving ecological sustainability and ensuring economic viability.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 1","pages":"Article 100229"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735123001245/pdfft?md5=5f8d67095341873bd7a43a573a48665b&pid=1-s2.0-S2772735123001245-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135705986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}