Water Biology and Security最新文献

{"title":"Evaluation of the influence of offshore wind farm noise on the fishes and dolphins in the Pearl River Estuary","authors":"Zhongchang Song ,&nbsp;Weijie Fu ,&nbsp;Hongquan Li ,&nbsp;Yingnan Su ,&nbsp;Zhanyuan Gao ,&nbsp;Wenxin Fan ,&nbsp;Jiangang Hui ,&nbsp;Wenzhan Ou ,&nbsp;Shengyao Sun ,&nbsp;Teng Wang ,&nbsp;Honghui Huang ,&nbsp;Yu Zhang","doi":"10.1016/j.watbs.2024.100318","DOIUrl":"10.1016/j.watbs.2024.100318","url":null,"abstract":"<div><div>In this study, we recorded the noise radiating from a wind farm and evaluated its potential impact on the fishes and Indo-Pacific humpback dolphins (<em>Sousa chinensis</em>) residing in the Pearl River Estuary. The pile driving pulses, recorded 30 ​m from the construction site, had a mean zero-to-peak sound pressure level of 195.1 ​dB (re 1 ​μPa) (<em>n</em> ​= ​238), exceeding the hearing thresholds of both fishes and Indo-Pacific humpback dolphins. The operational noise from the wind farm was significantly lower in amplitude; therefore, our analysis primarily focused on the radiation of pile driving noise. Acoustic measurements taken at three different distances during pile driving events supported the development of an acoustic propagation model, which was then used to predict the sound exposure levels of pile driving pulses radiating from 5 ​m below the water surface. By referring to established noise exposure metrics, we estimated an impact zone of 12.8 ​m for fishes. For the Indo-Pacific humpback dolphins, the permanent and temporary threshold shift zones were predicted to be 32.4 ​m and 580.9 ​m, respectively. Our findings underscore the importance of increasing our understanding on hearing sensitivities of the local inhabitants in the Pearl River Estuary and identify their core habitats. This approach enabled us to use the model to estimate impact zones and better protect the local marine life from the effects of pile driving noise radiation.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 1","pages":"Article 100318"},"PeriodicalIF":5.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hot and cold exposure triggers distinct transcriptional and behavioral responses in laboratory-inbred pond snails","authors":"Veronica Rivi ,&nbsp;Anuradha Batabyal ,&nbsp;Cristina Benatti ,&nbsp;Fabio Tascedda ,&nbsp;Johanna Maria Catharina Blom ,&nbsp;Ken Lukowiak","doi":"10.1016/j.watbs.2024.100315","DOIUrl":"10.1016/j.watbs.2024.100315","url":null,"abstract":"<div><div>Animals exhibit remarkable behavioral and molecular adaptations to cope with thermal stressors, which are crucial for survival in variable environments that are exacerbated by climate change. Aquatic poikilotherms like our model organism—the pond snail <em>Lymnaea stagnalis</em>—face significant challenges due to their dependence on external temperatures. Our study provides valuable insights into the different behavioral and molecular responses of lab-inbred snails to cold and heat shock stressors (i.e., 4 ​°C and 30 ​°C), particularly in the context of learning and memory formation. We found that while short-term (1 ​h) cold exposure transiently upregulated the expression levels of HSP70 and HSP40 in the snail's central ring ganglia, prolonged cold exposure (24 ​h) resulted in a significant downregulation of LymMIPII and an upregulation of LymMIPR. These data suggest, albeit at the transcriptional level, the existence of a negative feedback loop necessary for sustaining cellular functions when metabolic demands might shift towards conserving energy during prolonged cold exposure. At the behavioral level, we found that, compared to heat shock, cold exposure did not result in a Garcia effect (i.e., a “special form” of conditioned taste aversion). The difference in memory outcomes was associated with changes in the expression levels of selected targets involved in neuronal plasticity and the stress response. While both cold and heat shock upregulated the HSP levels in the snail's central ring ganglia, cold exposure did not affect the expression levels of the neuroplasticity genes LymGRIN1 and LymCREB1, contrasting with heat shock's neurogenic effects. Overall, this study provides insights into <em>L</em>. <em>stagnalis</em>'s adaptive responses to thermal stressors, emphasizing different molecular strategies for coping with heat versus cold challenges in aquatic environments. These findings contribute to our understanding of thermal biology and stress physiology in aquatic organisms, underscoring the importance of molecular mechanisms in shaping species' resilience in dynamic environments.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 1","pages":"Article 100315"},"PeriodicalIF":5.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seahorse nanos3 plays essential roles in germ cell development in the absence of nanos2","authors":"Lingzhen Chen ,&nbsp;Lu Tang ,&nbsp;Qiang Lin ,&nbsp;Yanhong Zhang","doi":"10.1016/j.watbs.2024.100312","DOIUrl":"10.1016/j.watbs.2024.100312","url":null,"abstract":"<div><div>Seahorses are specialized teleosts due to their unique male pregnancy and reproductive physiology. In particular, they have distinct gonad structures and different patterns of gametogenesis development in both sexes. However, the molecular mechanism of germ cell development in seahorses remains unknown. <em>Nanos</em>, which encodes conserved zinc-finger RNA-binding proteins, play essential roles for germline development in both vertebrates and invertebrates. In this study, we characterized the <em>nanos</em> gene family members and their potential roles in germline development in the lined seahorse, <em>Hippocampus erectus</em>. We showed that the <em>nanos</em> family members in the seahorse are the <em>nanos1a</em>, <em>nanos1b</em>, and <em>nanos3</em> genes, while <em>nanos2</em>, a key germline stem cell (GSC) maintenance factor, is intriguingly absent. More interestingly, <em>nanos3</em>, which is reported to be a female-specific gene, was also highly expressed in male gonads, as well as in the early developmental stages of testes and ovaries. Our results also showed that <em>nanos3</em> was highly expressed in early meiotic germ cells, and that expression levels of <em>nanos3</em> were lower in the gonad after TBT exposure. These findings suggest that <em>nanos3</em> may function during male germ cell development, and in female initial germ cell production – processes which are regulated by <em>nanos2</em> in other teleosts. This is the first research into how <em>nanos</em> governs germ cell development in an ovoviviparous fish. Our study provides insights into the regulatory mechanisms of germ cells development, as well as the early events during gametogenesis in the family <em>Syngnathidae.</em></div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 1","pages":"Article 100312"},"PeriodicalIF":5.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143171580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Black carp RNF115 restricts IRF3/7-mediated antiviral signaling in innate immunity","authors":"Yixuan He ,&nbsp;Qun Wang ,&nbsp;Lili Xiao ,&nbsp;Hui Wu ,&nbsp;Jun Xiao ,&nbsp;Jun Zou ,&nbsp;Hao Feng","doi":"10.1016/j.watbs.2024.100310","DOIUrl":"10.1016/j.watbs.2024.100310","url":null,"abstract":"<div><div>The Really Interesting New Gene (RING) ubiquitin E3 ligase family comprises a large number of members and plays a crucial role in the antiviral process. RING finger protein 115 (RNF115), also known as BCA2, Rabring7, or ZNF364, is a novel RING domain protein. In this paper, we cloned the RNF115 homologue from black carp (<em>Mylopharyngodon piceus</em>) and characterized it. The open reading frame of black carp RNF115 contains 933 nucleotides and encodes 310 amino acids. The C-terminal RING domain of RNF115 is highly conserved among various homologous species. Immunofluorescence assays revealed the cytoplasmic and nuclear distribution of RNF115 in the presence or absence of spring viremia of carp virus (SVCV) infection. Overexpression of RNF115 impaired interferon (IFN) and the related interferon-stimulated gene (ISG) mRNA expression, while upregulating SVCV replication. <em>Ex vivo</em> knockdown of RNF115 offered the host cells enhanced antiviral signaling. <em>In vivo</em> knockdown of RNF115 also strengthened black carp's antiviral capacity. Additionally, the results of a dual-luciferase reporter assay, plaque assay, and qRT-PCR assay demonstrated that co-transfection of RNF115 with IRF3/7 reduced IRF3/7-induced IFN transcription and antiviral ability. The association between RNF115 and IRF3/7 was detected by co-immunoprecipitation and immunofluorescence assays. Co-transfection of RNF115 with IRF3/7 also reduced the protein levels of IRF3/7, which were rescued by MG132. The enhanced K48-linked ubiquitination of IRF3/7 under the condition of RNF115 co-transfection implied the ubiquitin/proteasome degradation pathway catalyzed by RNF115. Cysteine 238 and 241 in the RING domain are the main enzyme active sites for RNF115, and the mutant C238/241A lost most of its ability to restrict IRF3/7. In conclusion, black carp RNF115 dampens IRF3/7-mediated IFN signaling through facilitating the ubiquitination and degradation of IRF3/7, which sheds light on the regulation of IFN signaling.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 1","pages":"Article 100310"},"PeriodicalIF":5.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial diversity and biogeography across gastrointestinal tracts of Takifugu pufferfish revealed by full-length 16S amplicon sequencing","authors":"Xingkun Jin ,&nbsp;Yan Shi ,&nbsp;Zhenlong Sun ,&nbsp;Yaohui Wang ,&nbsp;Zhe Zhao","doi":"10.1016/j.watbs.2024.100314","DOIUrl":"10.1016/j.watbs.2024.100314","url":null,"abstract":"<div><div>Fish, which are vital for both aquatic ecosystem functionality and global food supply, rely heavily on their gastrointestinal tract (GIT) microbiota for the digestion that underpins their growth and health. <em>Takifugu</em> pufferfish, which are an example of species evolved through adaptive radiation, possess a GIT that is specialized for antipredator defense and gluttonous feeding behaviors, offering a unique model to explore the effects of GIT compartmentalization and host genetics on gut microbial communities. Here we compiled 78 full and partial-length 16 ​S rRNA amplicon datasets across three anteroposteriorly distinct intestinal sites in a cohort of cohabitating artificial hybrid and purebred <em>Takifugu</em> pufferfishes. Our findings reveal a compositional and functional biogeography of pufferfish gut microbiota along the GIT and between host genetics. Additionally, the differential abundance of specific amplicon sequence variants and their correlation with host genetic backgrounds and intestinal sections highlight the role of environmental filtering in shaping microbial communities, with certain bacterial taxa exhibiting strong preferences for particular intestinal sites or genetic backgrounds, suggesting potential localized adaptation or functional specialization. This study enhances our understanding of the intricate interplay between host genetics, gut anatomy, and microbiota in fish, underscoring the importance of detailed microbial profiling in conservation efforts and aquaculture practices, and emphasizing the necessity of integrating full-length 16 ​S rRNA sequencing with partial-length datasets to comprehensively understand microbial diversity and function, paving the way for improved fish health management and sustainable aquaculture strategies.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 1","pages":"Article 100314"},"PeriodicalIF":5.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Muscle metabolism in response to oxidized fish oil feed in juvenile Nile tilapia","authors":"Xinyuan Li ,&nbsp;Lili Chen ,&nbsp;Hua Wen ,&nbsp;Xuan Wang ,&nbsp;Di Peng ,&nbsp;Jianmin Zhang ,&nbsp;Yangyang Liu ,&nbsp;Ming Jiang ,&nbsp;Lixue Dong ,&nbsp;Feng Huang ,&nbsp;Juan Tian","doi":"10.1016/j.watbs.2024.100321","DOIUrl":"10.1016/j.watbs.2024.100321","url":null,"abstract":"<div><div>To investigate the effect of oxidized fish oil on fish muscle metabolic responses and flesh quality, Nile tilapia (<em>Oreochromis niloticus</em>) weighing 13.73 ​± ​0.31 ​g were fed two diets for 12 weeks: a fresh fish oil (FFO) and a highly oxidized fish oil (OFO) diet. The peroxide value of the FFO and OFO diets was 2.2 meq/kg and 120.6 meq/kg, respectively. The OFO diet resulted in a decrease in growth, muscularity, nutritional value of fatty acids in the muscle, and density of myofibers. In the OFO group, the mRNA expression levels of lipolysis genes including lipoprotein lipase (<em>lpl</em>) and hormone-sensitive lipase (<em>hsl</em>) in the muscle were upregulated compared with the FFO group, while, the mRNA expression level of acetyl CoA carboxylase was downregulated. Additionally, under the positive ion mode, the levels of DHA deposition in phosphatidylcholine and phosphatidyl ethanolamine were reduced in the muscle of the OFO group compared to the FFO group. A total of 68 proteins were identified in the muscle, of which 42 were up-regulated and 26 were down-regulated. The KEGG pathway enrichment analysis revealed that the major pathways of the differentially abundant proteins were the Wnt signaling pathway, TGF-beta signaling pathway, and proteoglycans in cancer. In summary, the OFO diet negatively affected growth, muscularity, and nutritional value. It also inhibited the transformation of myofiber types and led to the apoptosis of myofibers.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 1","pages":"Article 100321"},"PeriodicalIF":5.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143171582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kernel density estimation of egg attachment areas aids in revealing spatiotemporal changes in Chinese sturgeon spawning grounds","authors":"Pengsheng Li ,&nbsp;Xuan Ban ,&nbsp;Jinming Wu ,&nbsp;Hui Zhang ,&nbsp;Junyi Li ,&nbsp;Li Shen ,&nbsp;Zhigang Liu ,&nbsp;Hao Du","doi":"10.1016/j.watbs.2024.100311","DOIUrl":"10.1016/j.watbs.2024.100311","url":null,"abstract":"<div><div>Identifying precise egg attachment areas and tracking trends of spawning magnitude (total amount of spawned eggs) are critical for accurate habitat assessment and effective conservation efforts, especially for lithophilic spawning fishes. However, accurate measurement of spawning conditions across both spatial and temporal dimensions poses significant challenges. We conducted a fourteen-year field study below the Gezhouba Dam, the main spawning ground for the Chinese sturgeon, using Kernel Density Estimation (KDE) method and Catch per Unit of Effort (<em>CPUE</em>) to refine knowledge on egg attachment areas relative to previous assessments. In addition, our analysis documented shifts in spawning locations within these four areas over the past fourteen years, revealing a worrying trend of decreasing spawning magnitude. This approach not only enabled the incorporation of the density distribution of eggs into the assessment of spawning magnitude trends, but also underscored the potential of the KDE as a framework for identifying egg attachment areas and estimating spawning magnitude trends. Our results provide valuable insights into spawning degradation of Chinese sturgeon and inform conservation strategies to protect their fragile spawning grounds.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 1","pages":"Article 100311"},"PeriodicalIF":5.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143170947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the mechanisms of hypoxia-induced tissue damage in fish: The role of GasderminEa/b in Larimichthys crocea","authors":"Lin Wang ,&nbsp;Shuaiyu Chen ,&nbsp;Yongyang Wang ,&nbsp;Weini Zhang ,&nbsp;Yinnan Mu ,&nbsp;Yudong Jia ,&nbsp;Changtao Guan ,&nbsp;Meng Xue ,&nbsp;Xinhua Chen","doi":"10.1016/j.watbs.2024.100322","DOIUrl":"10.1016/j.watbs.2024.100322","url":null,"abstract":"<div><div>Hypoxia is one of the major environmental stressors, frequently resulting in serious yield losses for maricultured large yellow croaker (<em>Larimichthys crocea</em>). We know that pyroptosis contributes to tissue damage under hypoxic conditions. However, whether GSDMEs-mediated pyroptosis is involved in hypoxia-induced tissue damage in fish remains unclear. In the present study, two <em>Lcgsdme</em> genes, <em>Lcgsdmea/b</em>, were cloned and characterized from the large yellow croaker. Both genes contain a conserved N-terminal pore-forming domain, a C-terminal autoinhibitory domain, and a flexible hinge region. After hypoxia stress, the expression of <em>Lcgsdmea/b</em> transcripts and proteins in the liver were significantly higher than in unstressed fish. The proteins of <em>Lc</em>GSDMEa/b could be cleaved under hypoxic conditions. Compared to <em>Lc</em>GSDMEb, the expression of <em>Lc</em>GSDMEa was higher in both mRNA and protein levels, thus exhibiting a stronger response during hypoxia stress. Furthermore, after 48 ​h of hypoxia stress, approximately 65% liver cells exhibited abnormalities, with pyroptosis being detected using a transmission electron microscope. TUNEL/<em>Lc</em>GSDMEa double staining assay revealed a high expression of <em>Lc</em>GSDMEa in the dead cells. We observed a significant up-regulation of pyroptosis pathway genes (<em>asc</em>, <em>caspase-3</em>) and pro-inflammatory cytokine genes (<em>il-1β</em>, <em>il-18</em>). After simultaneous knockdown of <em>Lcgsdmea/b</em> in vivo, the liver exhibited better health compared to the control group, with less cell swelling and vacuolation. Taken together, these findings demonstrate that hypoxia stress could activate <em>Lc</em>GSDMEa/b and induce pyroptosis in the liver of large yellow croakers, thereby contributing to tissue damage. Our study improves the understanding of hypoxia-induced tissue damage in fish, and provides new clues for protecting fish against hypoxia-induced damage.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 1","pages":"Article 100322"},"PeriodicalIF":5.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143100100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinct ecological processes shape micro-eukaryotic generalists and specialists in a canal-disturbed wetland, Lake Weishan, China","authors":"Xinghao Li ,&nbsp;Yunfeng Wang ,&nbsp;Micah Dunthorn ,&nbsp;Chengzhi Mao ,&nbsp;Qiaoyi Kong ,&nbsp;Saleh A. Al-Farraj ,&nbsp;Weibo Song ,&nbsp;Jie Huang","doi":"10.1016/j.watbs.2024.100320","DOIUrl":"10.1016/j.watbs.2024.100320","url":null,"abstract":"<div><div>Micro-eukaryotic communities are highly sensitive to environmental changes, and their structure offers crucial insights into the impact of human activities on aquatic habitats. Despite their significance, the assembly mechanisms of micro-eukaryotic communities in lake ecosystems heavily influenced by human activities are not well understood. In this study, we utilized 18S rDNA amplicon sequencing to examine the biodiversity of micro-eukaryotes over a one-year period in Lake Weishan, one of the largest freshwater lakes in northern China. We analyzed the ecological processes governing community assembly among key ecological groups, specifically habitat generalists and specialists. Our findings revealed substantial temporal variation in microbial diversity and community composition, likely driven by water temperature, dissolved oxygen, and the concentrations of several ions (e.g., Ca²⁺ and F⁻). Notably, community assembly in this canal-disturbed lake was predominantly influenced by stochastic processes (58.18%) rather than deterministic processes (20.46%). Habitat specialists (425 operational taxonomic units, OTUs) exhibited significantly greater diversity compared to habitat generalists (189 OTUs) and were predominantly shaped by stochastic processes (52.47%). In contrast, habitat generalists were less influenced by these processes (17.69%). Altogether, these results suggest that the ecological processes governing micro-eukaryotic community assembly differ markedly between habitat generalists and specialists, primarily due to their varied adaptability to environmental changes.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 1","pages":"Article 100320"},"PeriodicalIF":5.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143171579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary Bacillus pumilus on the growth, intestinal health, lipid metabolism, and mTOR signaling pathway of juvenile coho salmon (Oncorhynchus kisutch)","authors":"Mengjie Guo ,&nbsp;Liuqing Meng ,&nbsp;Fanghui Li ,&nbsp;Yongqiang Liu ,&nbsp;Ziqi Zhang ,&nbsp;Qin Zhang ,&nbsp;Weiguang Kong","doi":"10.1016/j.watbs.2024.100313","DOIUrl":"10.1016/j.watbs.2024.100313","url":null,"abstract":"<div><div>This study investigated the effects of dietary supplementation with the probiotic <em>Bacillus pumilus</em> on coho salmon (<em>Oncorhynchus kisutch</em>). A total of 360 juvenile fish, with an average initial weight of 130.75 ​± ​1.33 ​g, were randomly assigned to four treatment groups, each with three replicates of 30 fish. The fish were then fed for 70 days using formulated diets containing four concentrations of <em>B. pumilus</em>: 0 (control group), 2 ​× ​10⁶, 4 ​× ​10⁸, and 8 ​× ​10¹⁰ ​CFU/g. We then evaluated growth, nutrient metabolism, immune-related serum biochemical markers, enzyme activity, gene expression, and muscle composition. Additionally, we assessed intestinal histology and microbiota composition. Our findings revealed that <em>B. pumilus</em> significantly improved growth, protein and fat metabolism, and immune function compared to the control group. It also had a positive effect on the structure and function of the intestinal microbiota. The group receiving 4 ​× ​10⁸ ​CFU/g showed the greatest improvements. Overall, <em>B. pumilus</em> appears to be a promising probiotic for enhancing the health and performance of coho salmon in aquaculture.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 1","pages":"Article 100313"},"PeriodicalIF":5.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143171581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}