Water Biology and Security最新文献

{"title":"Environmental impact assessments should include rigorous scientific peer review","authors":"","doi":"10.1016/j.watbs.2024.100269","DOIUrl":"10.1016/j.watbs.2024.100269","url":null,"abstract":"<div><p>Twenty USA states or jurisdictions and 125 nations have modeled national environmental policies after the National Environmental Policy Act. That act mandates that federal agencies initiate environmental impact statements (EISs) when substantive environmental or human health consequences are likely because of an agency action related to proposed development projects. The science used to inform the EIS process, however, does not require independent scientific peer review (ISPR) in the USA or most other nations. But ISPR is needed for governments to accurately inform the EIS decision-making and public reporting processes. Instead, science is routinely manipulated during EIS reviews to generate expedient project outcomes with substantially negative ecological, political, and long-term economic consequences. We provide four examples of EISs that lack ISPR, as well as four examples where reviews by independent scientists were helpful to improve agency decisions. We also recommend that independent scientists (no affiliation with the project proponents or agencies overseeing projects) be used to help assess potential environmental and socio-economic impacts, as well as offer appropriate risk assessments, study designs, and monitoring timeframes. We conclude that nations should convene formal reviews using independent scientists as a form of peer review in the EIS process.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000301/pdfft?md5=69bd67fbbddbbcfa1d30ca936e4c6ef5&pid=1-s2.0-S2772735124000301-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141731967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An improved method for tubulin staining of ciliated eukaryotes","authors":"","doi":"10.1016/j.watbs.2024.100274","DOIUrl":"10.1016/j.watbs.2024.100274","url":null,"abstract":"<div><p>Ciliated microeukaryotes (ciliates) are distinguished by their cilia, which are rich in tubulin. We developed a method for tubulin staining in ciliate cells that involves using live-cell tubulin-staining dyes instead of antibodies thereby streamlining the staining process, which is effective across diverse ciliate lineages. Moreover, our method allows integration with immunofluorescence staining using antibodies when needed. The potential applications of this technique extend to cell biology and ciliate morphological and ecological studies.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000350/pdfft?md5=84f89319fcf12dd9cfbe56c612904f77&pid=1-s2.0-S2772735124000350-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141732043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A recombinase polymerase amplification with lateral flow assay for rapid on-the-spot detection of Aeromonas salmonicida","authors":"","doi":"10.1016/j.watbs.2024.100272","DOIUrl":"10.1016/j.watbs.2024.100272","url":null,"abstract":"<div><p><em>Aeromonas salmonicida</em> is a common pathogen of salmonid fishes that poses a significant threat to the fresh water and marine culture industry, potentially resulting in huge economic losses. To prevent and control fish diseases caused by <em>A. salmonicida</em>, rapid and effective diagnostic approaches must be developed, and which are important for routine monitoring and clinical care. By combining recombinase polymerase amplification (RPA) technology with a visible lateral flow strip (RPA-LF), we have enhanced both the precision of RPA detection and the convenience of real-time monitoring. In this study, we introduce a robust method for detecting <em>A. salmonicida</em> using RPA-LF. This assay specifically targets the ASA_1441 gene of <em>A. salmonicida</em>, ensuring high specificity, without cross-reactivity with other prevalent fresh water or marine pathogens. The optimal amplification temperature of the RPA assay was 39 ​°C. Its sensitivity extends to as low as 100 ​fg of purified DNA, representing more than 1000-fold higher sensitivity than conventional PCR methods. Furthermore, to enhance the usability of the RPA-LF assay, we developed a rapid sample preparation method using cellulose dipsticks for nucleic acid extraction. This method achieves a limit of detection (LOD) as low as 1.67 ​CFU/μL and completes the entire process within 20 ​min. In conclusion, our findings present a rapid and precise tool for monitoring <em>A. salmonicida</em> infection in aquaculture and marine culture. This advancement offers valuable insights for effective disease prevention and control strategies.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000337/pdfft?md5=126901cd7c2312fc1afeba1f805d4806&pid=1-s2.0-S2772735124000337-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141139412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity in freshwater scuticociliates (Alveolata, Ciliophora, Scuticociliatia): Two new species isolated from a wetland in northern China, focusing on their morphology, taxonomy, and molecular phylogeny, with establishment of a new family Glauconematidae fam. nov.","authors":"","doi":"10.1016/j.watbs.2024.100275","DOIUrl":"10.1016/j.watbs.2024.100275","url":null,"abstract":"<div><p>In the aquatic microbial food web, scuticociliates are diverse and predominant in almost all kinds of biotopes while extremely confused regarding their taxonomy and systematic relationships within the subclass Scuticociliatia. Here we provide detailed descriptions of two new and one known species, <em>Glauconema sinica</em> sp. nov., <em>Pleuronema parasetigerum</em> sp. nov., and <em>Histiobalantium natans viridis</em>, isolated from a freshwater wetland in northern China, along with their molecular phylogeny based on SSU rRNA gene sequences. Our results show: (1) both molecular and morphological data strongly support that the isolated lineage containing the genera <em>Glauconema</em>, <em>Miamiensis</em>, <em>Paramesanophrys</em>, and <em>Anophryoides</em> should be identified as a new taxon at the family level in the order Philasterida; hence, a new family, Glauconematidae fam. nov., is established. This new family is mainly characterized by the polymorphic life cycle of its members (macrostome and microstome stages); the trophont (or macrostome) has closely spaced membranelles 1–3, whereas in the tomite (or microstome) membranelle 1 is distant from membranelle 2. (2) Phylogenetic analyses using novel data for these species revealed that all three cluster with their congeners, supporting the validity of the genera to which they belong. Molecular information also supports the monophyly of the two main scuticociliate orders Pleuronematida and Philasterida.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000362/pdfft?md5=d440f26da58f3a6edfafb53c9107b4d2&pid=1-s2.0-S2772735124000362-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141391786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thirty years of change: Assessing the dynamics of fish communities in Daya Bay, a semi-enclosed coastal ecosystem of the South China sea","authors":"","doi":"10.1016/j.watbs.2024.100268","DOIUrl":"10.1016/j.watbs.2024.100268","url":null,"abstract":"<div><p>Daya Bay is a semi-enclosed bay in the South China Sea which is a coastal ecosystem with significant ecological and economic value that is subjected to a variety of anthropogenic disturbances. However, long-term variation in fish community structure in this Bay remains uncertain. In this study, we aimed to explore the changing characteristics of fish species composition and community structure in Daya Bay during the past 30 years using data from 1989 to 2021 from bottom trawl surveys and historical literature data. As of now, a total of 544 species of fish have been identified in the Bay, belonging to 24 orders and 125 families. The structure of the fish community in the bay has changed over time. Firstly, there has been a clear decrease in the number of species and biomass of large fish. Secondly, the number of fish constituent orders and families has decreased. Moreover, both the average taxonomic distinctness and variation in taxonomic distinctness decreased from 1989 to 2021. Thirdly, there is low similarity among fishes at different time phases and distinct changes in the composition of dominant species. Present results indicate that the fish community structure in Daya Bay has tended to reflect a decline in diversity, shifts in composition, and shrinking of fish body size in the past 30 years. This study improves our understanding of fish species composition and its drivers over time in Daya Bay, providing important knowledge to support the protection and restoration of this ecosystem and the important ecosystem services it provides.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000246/pdfft?md5=5aaea382f3483608d5f68bd1e7a47ae9&pid=1-s2.0-S2772735124000246-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140768914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species composition and succession of coral reef fishes in Huaguang Reef, Xisha Islands","authors":"","doi":"10.1016/j.watbs.2024.100273","DOIUrl":"10.1016/j.watbs.2024.100273","url":null,"abstract":"<div><p>A list of coral reef fish species of Huaguang Reef was compiled in 2023 using hand fishing, diving fishing, underwater video, and eDNA detection, combined with historical data from 1979 and archival records from the South China Sea Fisheries Research Institute of the Chinese Academy of Fisheries Sciences for the years of 1998, 1999, 2003, and 2005. Successional characteristics of coral reef fishes through time were also explored. The findings revealed: (1) A total of 299 coral reef fish species were identified in Huaguang Reef, categorized into 2 classes, 16 orders, and 56 families, with Perciformes exhibiting the highest species count (234), constituting 78.26% of all species. (2) In comparison to the period of 1979–2005, there was a reduction in the number of species at the order and family levels in 2023 at Huaguang Reef. Both the average taxonomic distinctness (Δ+) and the variation in taxonomic distinctness (Λ+) exhibited a declining trend, indicating disturbances in the fish ecosystem. (3) The proportion of herbivorous fish at Huaguang Reef remained relatively stable in 2023 compared to the 1979–2005 period. Notably, the similarity coefficient of herbivorous fish was the highest among different feeding types, signifying a degraded state of the Huaguang Reef habitat. (4) In contrast to the 1979–2005 period, a significant decline was observed in large-sized and carnivorous fish species at Huaguang Reef in 2023. The low similarity coefficients for both categories, with undiscovered fish species accounting for 58.49% and 45.76% of their respective taxa in the total list, respectively, suggested a notable impact of overfishing on coral reef fishes. The succession patterns revealed in this study provide a theoretical foundation for advancing the sustainable development of coral reef fish resources in the Xisha Islands and offer valuable insights for the protection and management of coral reef fishes.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000349/pdfft?md5=9dcef2d93de6d800ed80024bae1600f3&pid=1-s2.0-S2772735124000349-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141731969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatiotemporal variation of microeukaryotic biodiversity and biotic condition associated with disinfectants after the COVID-19 lockdown in Wuhan, China","authors":"","doi":"10.1016/j.watbs.2024.100266","DOIUrl":"10.1016/j.watbs.2024.100266","url":null,"abstract":"<div><p>Intensive application of chlorine-based disinfectants driven by the COVID-19 pandemic was suspected to be detrimental to receiving water ecosystems, but with little field evidence. We characterized the occurrences of typical disinfectants and microeukaryotic communities in surface waters associated with three wastewater treatment plants (WWTP)-river systems one year after the lockdown in Wuhan, China. Trihalomethanes (THMs) declined from summer 2020 (0.01–1.82 ​μg/L) to summer 2021 (0.01–0.95 ​μg/L), whereas quaternary ammonium salts (QAs) increased from summer 2020 (0.53–6.35 ​ng/L) to summer 2021 (8.49–191 ​ng/L). Biodiversity monitoring with environmental DNA (eDNA) revealed significant temporal variation in microeukaryotic community composition. The monitored disinfectants were correlated with some eukaryotic communities as demonstrated by redundancy analysis. For example, QAs were positively related to Cryptophyta relative abundance, but negatively related to Rotifera relative abundance. A microeukaryote-based multimetric index indicated ecological impairment near the Han River WWTP outlet in 2020. Our findings indicate the influence of heavily used disinfectants on river microeukaryotic communities, and the usefulness of assessing mid-term ecological risks from disinfectants in the post COVID-19 era.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000222/pdfft?md5=63bdc043a6d4cc836bb79b061bc7cd04&pid=1-s2.0-S2772735124000222-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140796949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chinese wisdom and modern innovation of aquaculture","authors":"","doi":"10.1016/j.watbs.2024.100271","DOIUrl":"10.1016/j.watbs.2024.100271","url":null,"abstract":"<div><p>Aquaculture originates from ancient Chinese wisdom, and is considered the most efficient food-producing industry. About 70 years ago, New China proposed an industrial approach to fish production and made a policy decision to begin modern aquaculture development. As one of lucky persons since the early 1980s, I have committed my scholarly career to the studies on genetics and breeding biotechnology of polyploid gibel carp (<em>Carassius gibelio</em>). Here, several significant recent innovations, such as the proposition of the amphitriploid concept, the association of ploidy changes with reproduction transition, the finding of a unique ameio-fusiongenesis mode, the establishment of a controllable fertility approach, the creation of intermuscular bone-free mutants, as well as the recreation of desirable goldfish, are introduced and emphasized from an active participant of these innovations. Additionally, some modern innovations and contributions are also highlighted in other aspects of aquaculture including feed development, disease control, facilities and equipment upgrading, as well as paradigm and mode changes. This comprehensive synthesis of ancient Chinese wisdom and modern innovations seeks to provide valuable insights into the field from a historical basis.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000325/pdfft?md5=0ea102eb650fc1b27c077fb532e7be26&pid=1-s2.0-S2772735124000325-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141030189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SOCS3 acts as a potential negative regulator in the antiviral response of large yellow croaker (Larimichthys crocea) by interacting with STAT1","authors":"","doi":"10.1016/j.watbs.2024.100270","DOIUrl":"10.1016/j.watbs.2024.100270","url":null,"abstract":"<div><p>Suppressors of cytokine signaling (SOCS) proteins are important regulators of the Janus kinase-signal transducer and activator of transcription (JAK-STAT) pathway. Within the SOCS family, SOCS3 is one of the most potent inhibitors of cytokine signaling. However, there is limited knowledge regarding the function of SOCS3 on regulating type I interferon (IFN) signaling in fish. In this study, the complete open reading frame (ORF) of <em>SOCS3</em> from the large yellow croaker (<em>Larimichthys crocea</em>, <em>LcSOCS3</em>) was cloned and characterized. The ORF of <em>LcSOCS3</em> was 618 nucleotides in length and encoded a protein containing 205 amino acids. <em>Lc</em>SOCS3 had the typical domain architecture of the SOCS family, including an SRC homology 2 (SH2) domain, a SOCS box, an additional kinase inhibition region (KIR), and an extended SH2 subdomain (ESS). Phylogenetic analysis revealed that <em>Lc</em>SOCS3 was clustered with other fish SOCS3s and most closely related to the SOCS3 of <em>Collichthy lucidus</em>. <em>LcSOCS3</em> mRNA was detected in all organs or tissues examined, and its expression was significantly increased in both head kidney and spleen tissues, and primary head kidney leukocytes after poly(I:C) stimulation. Overexpression of <em>Lc</em>SOCS3 significantly promoted Spring viremia of carp virus (SVCV) replication, resulting in a more severe cytopathic effect, increased viral titer, enhanced copy number of the SVCV-G gene, and decreased expression levels of <em>IFN1</em>, <em>IRF7</em>, <em>ISG15</em>, <em>Viperin</em>, <em>PKR</em>, and <em>Mx</em> in epithelioma papulosum cyprinid (EPC) cells. Silencing of <em>LcSOCS3</em> correspondingly up-regulated the expression of <em>IFNi</em>, <em>IFNh</em>, <em>PKR</em>, <em>Viperin</em>, and <em>Mx</em> in large yellow croaker head kidney (LYCK) cells. Additionally, <em>Lc</em>SOCS3 was shown to interact with Signal Transducer and Activator of Transcription 1 (STAT1) which may inhibit STAT1 translocating into the nucleus. This speculation was supported by the increased phosphorylation level of STAT1 in head kidney leukocytes after <em>Lc</em>SOCS3 silencing. These results indicated that <em>Lc</em>SOCS3 functioned as a potential negative regulator of type I IFN signaling in large yellow croaker through its interaction with STAT1.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000313/pdfft?md5=b52ac5b2108067d0451a152fff75c601&pid=1-s2.0-S2772735124000313-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141051371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular matrix (ECM) pathway involved in skin immune response of Cynoglossus semilaevis upon Vibrio vulnificus infection","authors":"","doi":"10.1016/j.watbs.2024.100277","DOIUrl":"10.1016/j.watbs.2024.100277","url":null,"abstract":"<div><p>Half-smooth tongue sole (<em>Cynoglossus semilaevis</em>) is regarded as a significant commercial marine fish species in China, and frequent outbreaks of vibriosis has led to substantial economic losses<em>.</em> In this study, we investigated the molecular mechanisms underlying the involvement of the extracellular matrix (ECM) signaling pathway in the skin immune response of <em>C. semilaevis</em> infected with <em>Vibrio vulnificus</em>. The results showed that most differentially expressed proteins (DEPs) were identified through iTRAQ proteome analysis, and ten ECM-related proteins were screened at 12 and 36 ​h post-infection (hpi). Notably, several DEPs associated with ECM including HSPG, FN, COCH, LAMB1, LAMC1, TSPN, COL6A1, AMBP, HX and ITGA6 were tested for their expression at the transcriptional level during <em>V. vulnificus</em> infection using qRT-PCR assay. The analysis of protein-protein interaction (PPI) showed that the integrin ITGA6 exhibited obvious interactions among ECM-related DEPs. Furthermore, the spatio-temporal expression of the <em>Csitga6</em> gene was highest in the skin, gill, muscle, and spleen, but lower in the liver, kidney and intestine. To our knowledge, this is the first report on the involvement of ECM pathway in the skin immune response to <em>V. vulnificus</em> infection, and provides a reference for further study of the ECM mechanism in the mucosal immune response of marine fish.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000386/pdfft?md5=961ca472f3cc76973d6b0b2aa11e2011&pid=1-s2.0-S2772735124000386-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141731968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}