Water Biology and Security最新文献

{"title":"Do fish in an urban river system use a rehabilitated and reconnected stormwater pond during the summer?","authors":"Jessica L. Reid ,&nbsp;Gillian Zorn ,&nbsp;Sam Woods ,&nbsp;Jennifer Lamoureux ,&nbsp;Sean J. Landsman ,&nbsp;Jonathan D. Midwood ,&nbsp;Steven J. Cooke","doi":"10.1016/j.watbs.2024.100333","DOIUrl":"10.1016/j.watbs.2024.100333","url":null,"abstract":"<div><div>For mobile animals such as fish, different habitat types at various life stages are required to support a broad range of ecological requirements. However, in urban areas, access to suitable habitat may be impeded. Moreover, rehabilitation efforts focused on urban waterbodies are infrequent, small-scale, and inadequately monitored. We characterized the connectivity of the fish community between the Jock River in eastern Ontario and a rehabilitated stormwater management pond during late spring. Passive integrated transponder (PIT) tags were implanted into over 800 individual fish, representing sixteen species with a wide range of body sizes and life-histories. Summer residency and behavior were assessed using detections from an array of autonomous PIT receiver stations. This research revealed that there is ecological connectivity between an adjacent rehabilitated stormwater pond and the Jock River. Some species were resident in the pond during the entire summer monitoring period while others such as sunfish (<em>Lepomis</em> sp.) and rock bass (<em>Ambloplites rupestris</em>) made regular forays between the river and the pond. There was also evidence of significant movement within the pond for species such as golden shiner (<em>Notemigonus crysoleucas</em>) and northern pike (<em>Esox lucius</em>). This study demonstrates the potential for created habitats in floodplain areas initially intended for stormwater management to be rehabilitated to provide significant ecological value in urbanized watersheds.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 2","pages":"Article 100333"},"PeriodicalIF":5.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inside Front cover - Contents","authors":"","doi":"10.1016/S2772-7351(25)00033-2","DOIUrl":"10.1016/S2772-7351(25)00033-2","url":null,"abstract":"","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 2","pages":"Article 100390"},"PeriodicalIF":5.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-144 targets Nrf2 affecting the intestinal oxidative damage induced by oxidized fish oil in Megalobrama amblycephala with emphasis on autophagy and apoptosis","authors":"Jie Yang ,&nbsp;Xiaochuan Zheng ,&nbsp;Xin Liu ,&nbsp;Qunlan Zhou ,&nbsp;Cunxin Sun ,&nbsp;Changyou Song ,&nbsp;Aimin Wang ,&nbsp;Bo Liu","doi":"10.1016/j.watbs.2024.100340","DOIUrl":"10.1016/j.watbs.2024.100340","url":null,"abstract":"<div><div>To explore the modulatory role of <em>miR-144</em> in <em>Nrf2</em> cascade reactions and assess its potential as a target for alleviating oxidative fish oil-induced intestinal damage in <em>Megalobrama amblycephala</em>, four experimental diets were formulated. A conventional diet was kept as the control diet (NC), while experimental diets consisted of the control diet supplemented with oxidized fish oil (OF, peroxide value: 323 ​mmol/kg), OF ​+ ​<em>miR-144</em> agomir and OF ​+ ​<em>miR-144</em> antagomir, respectively. In the oxidative stress model of <em>M.</em> <em>amblycephala</em> induced by oxidized fish oil, dihydroethidium (DHE) fluorescence probe and morphological results revealed that oxidized fish oil markedly elevated the level of reactive oxygen species (ROS) within intestinal cells and destroyed the intestinal barrier structure, such that the height of the intestinal villus and the thickness of the muscle layer were significantly reduced, and intestinal barrier-related genes <em>ZO-1</em>, <em>Occludin</em> and <em>Claudin-7α</em> were significantly down-regulated. Additionally, oxidized fish oil treatment activated autophagy and apoptosis of intestinal cells, which was manifested by the significant up-regulation of autophagy-related genes <em>Beclin-1</em>, <em>VPS34</em>, <em>P62</em> and <em>ATG5</em> (<em>P</em> ​&lt; ​0.05), the increase of autophagosomes, and the significant up-regulation of apoptosis-related genes <em>HSP27</em>, <em>Caspase3</em>, <em>Caspase8</em> and <em>Caspase9</em> (<em>P</em> ​&lt; ​0.05). Further studies showed that <em>miR-144</em> agomir significantly enhanced <em>Keap1</em> expression and inhibited <em>Nrf2</em> expression, while <em>miR-144</em> antagomir had the opposite effect. Importantly, forced expression of <em>miR-144</em> induced ROS accumulation by silencing <em>Nrf2</em>, raised the expression of inflammatory factors (<em>P</em> ​&lt; ​0.05), and even further induced the development of autophagy and apoptosis, while <em>miR-144</em> antagomir could offset this effect by reactivating <em>Nrf2</em> expression. In summary, this study showed that silencing <em>miR-144</em> could protect intestinal cells of <em>M.</em> <em>amblycephala</em> from oxidative damage by targeting the <em>Nrf2</em> signaling pathway. Therefore, <em>miR-144</em> is expected to be a regulatory target for ameliorating intestinal oxidative damage induced by oxidized oils.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 2","pages":"Article 100340"},"PeriodicalIF":5.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell RNA sequencing of zebrafish olfactory epithelium reveals cellular heterogeneity and responses to a conspecific alarm substance","authors":"Wenjun Chen ,&nbsp;Haifeng Jiang ,&nbsp;Cheng Wang ,&nbsp;Zufa Ding ,&nbsp;Dan Yu ,&nbsp;Yang Liu ,&nbsp;Shunping He ,&nbsp;Liandong Yang","doi":"10.1016/j.watbs.2024.100324","DOIUrl":"10.1016/j.watbs.2024.100324","url":null,"abstract":"<div><div>Olfaction, the sense of smell, is vital for the survival of many species and serves as an excellent system for investigating the molecular basis of behavior. Fishes possess a well-developed olfactory system that governs various behaviors related to feeding, reproduction, and fear. However, the cellular diversity and heterogeneity of the fish olfactory epithelium remains largely unexplored. This study presents a single-cell atlas of the zebrafish olfactory epithelium using single-cell RNA sequencing (scRNA-seq). Through scRNA-seq analysis of approximately 10,587 ​cells, we identified nine distinct cell types with unique transcriptional profiles, including immature and mature olfactory sensory neurons (OSNs), horizontal basal cells, globose basal cells, and sustentacular cells, as well as lymphocyte and myeloid cells expressing immune signals. Further subcluster analysis revealed selective and combinatorial expression of key components in odorant-mediated signal transduction by distinct OSN populations. Additionally, we discovered transcriptional changes specific to certain OSN populations following exposure to a conspecific alarm substance. The single-cell transcriptional atlas of the zebrafish olfactory epithelium provided in this study serves as a valuable tool for exploring cell diversity and assessing genetic profiles from functional and behavioral perspectives in fish.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 2","pages":"Article 100324"},"PeriodicalIF":5.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ensuring water purity: Mitigating environmental risks and safeguarding human health","authors":"Ovinuchi Ejiohuo ,&nbsp;Helen Onyeaka ,&nbsp;Adenike Akinsemolu ,&nbsp;Ozioma Forstinus Nwabor ,&nbsp;Kehinde Favour Siyanbola ,&nbsp;Phemelo Tamasiga ,&nbsp;Zainab T. Al-Sharify","doi":"10.1016/j.watbs.2024.100341","DOIUrl":"10.1016/j.watbs.2024.100341","url":null,"abstract":"<div><div>Water is an indispensable resource for sustaining life, ecosystems, and economic productivity. However, increasing contamination and pollution pose severe threats to global water purity, with significant risks to human health, biodiversity, and ecosystem stability. This study examines the diverse dimensions of water purity, including its impacts on ecosystems and human health, sources of pollution, global disparities, regulatory frameworks, and technological advancements. These factors are essential in addressing the escalating threats to water quality and public health. This study also explores the complex relationship between water contamination and food security, emphasising the role of water purification in disease prevention and sustainable food systems. It underscores the critical need for effective water purification strategies, exploring challenges and potential solutions, including applications of artificial intelligence and big data. The study highlights mechanisms to enhance transparency and accountability in water resource management through an analysis of policy frameworks and innovative practices. By evaluating the effectiveness of current purification methods and regulatory standards, the study provides valuable insights for sustainable water protection initiatives. Findings from this study highlight global water access and quality disparities, and suggests the need for targeted strategies to promote equity and a coordinated, international response to safeguard water resources. This study offers robust theoretical support for environmental protection policies and advocates for stronger local capabilities in addressing regional water challenges. It delivers a comprehensive framework to mitigate pollution risks, protect biodiversity, and promote public health, underscoring the global urgency of securing clean water access.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 2","pages":"Article 100341"},"PeriodicalIF":5.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Passage efficiency and behavioral performance of Schizothorax davidi through different sections of a long vertical slot fishway","authors":"Jianghui Bao ,&nbsp;Xiang Wang ,&nbsp;Weiwei Li ,&nbsp;Chaoshuo Zhang ,&nbsp;Xiangyuan Mi ,&nbsp;Dongxu Zhang ,&nbsp;William M. Twardek ,&nbsp;Hsien-Yung Lin ,&nbsp;Ye Qiao ,&nbsp;Steven J. Cooke ,&nbsp;Ming Duan","doi":"10.1016/j.watbs.2024.100330","DOIUrl":"10.1016/j.watbs.2024.100330","url":null,"abstract":"<div><div>Fish passage facilities are constructed worldwide to enable fish to pass anthropogenic obstacles (i.e., dams) and re-establish river connectivity. The construction of these facilities involves sophisticated engineering designed to attract fish and enable passage. The behavior of fish encountering these structures, particularly in long vertical slot fishways, has been poorly studied. This study was conducted on the Daduhe River in Southwest China to quantify the upstream passage efficiency and performance of tagged <em>Schizothorax davidi</em> in different sections of a long vertical slot fishway spanning 1228.3 ​m. The overall passage efficiency was 13% although the passage efficiency in seven sections (A0-A1, A1-A2, A2-A3, A3-A4, A4-A5, A5-A6, A6-A7) ranged from 43% to 100% reflecting differences in slope among sections. The highest passage efficiency was documented in rest pool sections with a slope of 0 (A3–A4, 100% and A6–A7, 100%) and during passage through the dam itself (A5–A6, 90.0%). The lowest passage efficiency was section A2–A3, where a garbage interception facility affected passage efficiency. Average transit time from A0 to A7 was 85.2 ​h and ranged from 8.8 to 237.6 ​h. Transit speed varied over various sections. The first section (i.e., A0–A1) had slow transit speeds which presumably was influenced by acclimation and recovery from tag implantation. After this, the transit speed had a slight increase but decreased again. Additionally, more than half (5 out of 9 fish) of the fish fell back after reaching the most upstream section and reascended the fishway which tripled the total passage time for those individuals. No significant diel activity rhythms were observed, but a strong bimodal distribution was noted in fish transit time when passing the first monitoring site. Cox-proportional hazards model showed that transit time was negatively correlated with body size but positively correlated with water level and water temperature. Our study provides valuable insights into activity when passing through a long fishway, which can inform design of fishway structures and operational patterns to improve upstream passage efficiency. Specifically, we recommend increasing the number of resting pools and maintaining appropriate water levels to reduce transit time and fallback frequency in long fishways.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 2","pages":"Article 100330"},"PeriodicalIF":5.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Density-dependent effects of zooplanktivorous Thin Sharpbelly (Toxabramis swinhonis) on plankton assemblages and water quality: Implications for lake rehabilitation","authors":"Chao Guo ,&nbsp;Wei Li ,&nbsp;Adam G. Hansen ,&nbsp;Jie Ke ,&nbsp;Shiqi Li ,&nbsp;Chuansong Liao ,&nbsp;Xingwei Cai ,&nbsp;Jing Yuan ,&nbsp;Jiashou Liu","doi":"10.1016/j.watbs.2024.100331","DOIUrl":"10.1016/j.watbs.2024.100331","url":null,"abstract":"<div><div>Because of anthropogenic and natural factors, zooplanktivorous fish often dominate fish assemblages within lake ecosystems along the middle and lower reaches of the Yangtze River Basin (MLYRB), China. Despite their prevalence, the density-dependent effects of zooplanktivorous fish on lake food webs within the MLYRB are poorly understood compared to other omnivorous fish. We conducted a mesocosm experiment to quantify how plankton communities and water quality responded to varying densities of a common zooplanktivorous fish (Thin Sharpbelly <em>Toxabramis swinhonis</em>). During the experiment, the mean weekly total nitrogen (TN), total phosphorus (TP), ammonium nitrogen (NH₄⁺-N), chemical oxygen demand (COD_Mn), turbidity, and chlorophyll-<em>a</em> (Chl.<em>α</em>) values in treatments where Thin Sharpbelly were present (i.e., fish-present) were all significantly higher than those in the control treatment with no Thin Sharpbelly (i.e., fish-absent). In addition, all water quality variables exhibited a significant positive relationship with the density of Thin Sharpbelly. The abundance and biomass of phytoplankton in the fish-present treatments were significantly higher than in the fish-absent treatment, with <em>Pseudanabaena</em> spp., <em>Dolichospermum</em> spp., and <em>Limnothrix</em> spp. dominating the phytoplankton assemblage. The biomass and abundance of small-bodied zooplankton increased with fish density, whereas the relative abundance and biomass of large-bodied zooplankton (cladocerans and copepods) showed the opposite trend. Also, the ratio of zooplankton to phytoplankton biomass declined nonlinearly with fish density, while the ratio of Chl.α to TP was significantly positively correlated with fish density. Collectively, experimental results indicated that zooplanktivorous fish increased the concentrations of nutrients and stimulated phytoplankton growth through feeding on large zooplankton and the strength of these effects increase with fish density. Moreover, results indicate that high densities of zooplanktivorous fish can alter plankton assemblage structure by facilitating growth of undesirable cyanobacteria and shifting the composition of zooplankton to smaller-bodied species and forms. Our results demonstrate how Thin Sharpbelly, and potentially other obligate zooplanktivorous fish, can have adverse effects on water quality and plankton assemblages, but these responses are density dependent. Our findings suggest that managing the density of zooplanktivorous fish could be a useful ecological rehabilitation measure for improving water quality in MLYRB lakes.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 2","pages":"Article 100331"},"PeriodicalIF":5.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome size affects phytoplankton response to environmental factors: A preliminary exploration in 26 lakes in the region east of the Hu Huanyong Line","authors":"Wei Wang ,&nbsp;Hanjie Huang ,&nbsp;Kangxu Zhao ,&nbsp;Junping Lv ,&nbsp;Xudong Liu ,&nbsp;Ying Shi ,&nbsp;Shulian Xie ,&nbsp;Jia Feng","doi":"10.1016/j.watbs.2024.100339","DOIUrl":"10.1016/j.watbs.2024.100339","url":null,"abstract":"<div><div>Given their varied physiological and ecological attributes, phytoplankton frequently exhibit disparate responses to changes in environmental conditions. Therefore, when studying modifications in the structure of a phytoplankton community, it is necessary to consider such differences and investigate the underlying mechanisms. Through a field sampling study of 26 lakes, we found that the differing responses of phytoplankton to changes in environmental factors were partly related to their genome size. The Cyanobacteria and Chrysophyta, which possess smaller genomes, exhibited a relatively low degree of sensitivity to alterations in nutrient concentrations. However, they demonstrated a stronger dependence on the metabolic processes of environmental microorganisms compared to other phytoplankton with larger genomes. Furthermore, the Cyanobacteria and Chrysophyta were more closely associated with environmental nutrients and water chemistry and were more actively involved in interspecific interactions among phytoplankton. By randomly deleting sample points, we verified the generality of the above conclusions. However, more refined grouping analyses indicate that diatoms exhibited different ecological characteristics due to their physiological and morphology. The present study offers new insights into the mechanisms underlying the ecological adaptations of phytoplankton and may provide new guidance for the management of Cyanobacterial blooms. Further studies could take a more refined approach to investigate the effect of phytoplankton “effective genome size” on their adaptive capacity.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 2","pages":"Article 100339"},"PeriodicalIF":5.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organelle and transcriptomic genomes of a freshwater brown alga and further insights on the phylogenetic relationship of red secondary plastid-derived lineages","authors":"Fangru Nan,&nbsp;Tao Zhang,&nbsp;Huiying Zhao,&nbsp;Jia Feng,&nbsp;Junping Lv,&nbsp;Qi Liu,&nbsp;Xudong Liu,&nbsp;Yang Liu,&nbsp;Shulian Xie","doi":"10.1016/j.watbs.2024.100328","DOIUrl":"10.1016/j.watbs.2024.100328","url":null,"abstract":"<div><div>Freshwater brown algae have been acknowledged as the least diverse of all groups of freshwater algae. Morphological and molecular investigation were conducted on a freshwater brown alga collected in this study. Morphological characterization and phylogenetic tree based on the <em>rbc</em>L gene confirmed its attribution to <em>Heribaudiella fluviatilis.</em> The complete organelle genomes of <em>H. fluviatilis</em> were determined and assembled from high-throughput sequencing. A circular tetrad structure of 128,880 bp was identified for the chloroplast, and the mitochondrial genome was assembled as a circle with 43,657 bp in length. This study provides the first and only record of organelle genomic information of the freshwater Phaeophyceae, which are important for understanding the endosymbiosis theories. Concatenated (Bayesian inference, maximum likelihood under MFP and GHOST models) and coalescent methods were used to construct the phylogenetic relationship among secondary plastid-bearing lineages based on organelle genomes. Obvious conflicts were observed in grouping relationships of chloroplast and mitochondrial genomes, even among different evolutionary models and tree-constructing methods. The transcriptome of <em>H. fluviatilis</em> was sequenced and used to construct the phylogenetic relationship among secondary plastid-bearing lineages. The phylogenetic relationships in nuclear and mitochondrial genomes were consistent in grouping CHSR taxa (cryptophytes, haptophytes, stramenopiles and rhizarians) in an independent branch. The phylogenetic relationship constructed from nuclear transcriptomes was highly reliable by owning robust supporting values on each node and was consistent with the serial endosymbiosis theory on evolution of red secondary plastid-derived lineages.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 2","pages":"Article 100328"},"PeriodicalIF":5.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and functional characterization of IGFBP genes in Leopard Coral Grouper (Plectropomus leopardus): Insights into growth regulation and stress response","authors":"Yiwen Sun ,&nbsp;Chaofan Jin ,&nbsp;Shaoxuan Wu ,&nbsp;Chenlin Yin ,&nbsp;Jianmei Chen ,&nbsp;Zhenmin Bao ,&nbsp;Bo Wang ,&nbsp;Jingjie Hu","doi":"10.1016/j.watbs.2024.100338","DOIUrl":"10.1016/j.watbs.2024.100338","url":null,"abstract":"<div><div>The Insulin-like Growth Factor Binding Protein (IGFBP) family genes, which function as carrier proteins in the circulation, are integral to various physiological processes such as growth, metabolism, reproduction, and stress responses. However, the systematic identification, characterization, and functional analysis of <em>igfbp</em> family genes in teleosts remain to be explored. In this study, we identified and characterized a total of 11 IGFBP family members in the Leopard Coral Grouper (<em>Plectropomus leopardus</em>). Expression analysis revealed that <em>igfbp</em> genes exhibit tissue-specific expression patterns in adult <em>P. leopardus</em>. Notably, certain <em>igfbp</em> genes were differentially expressed in growth-related tissues in fast-growing versus slow-growing individuals, suggesting their potential role in growth regulation. Specifically, the expression of <em>igfbp2a</em> and <em>igfbp2b</em> in the liver was further validated using FISH. Furthermore, knockdown of <em>igfbp2b</em> expressed specifically in the liver resulted in the promotion of muscle development and increased expressions related to muscle cell development. Additionally, several <em>igfbp</em> genes were dynamically involved in the response to acute hypothermia in the brain, gills, kidneys, and liver. These findings offer a comprehensive understanding of the characteristics and functions of <em>igfbp</em> family genes in <em>P. leopardus</em>, providing a solid foundation for further investigation into the functional mechanisms of <em>igfbp</em> genes in teleosts.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 2","pages":"Article 100338"},"PeriodicalIF":5.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}