Water Biology and Security最新文献

{"title":"Environmental microbes determine macrophage response towards saponin-induced inflammation in zebrafish larvae","authors":"Adrià López Nadal ,&nbsp;Evelien Kidess ,&nbsp;Jos Boekhorst ,&nbsp;Geert Wiegertjes ,&nbsp;Sylvia Brugman","doi":"10.1016/j.watbs.2023.100187","DOIUrl":"https://doi.org/10.1016/j.watbs.2023.100187","url":null,"abstract":"<div><p>The microbial consortium within an organism is crucial for its development and immune status. Alteration of the host microbiome by antibiotics or antinutritional factors may contribute to increased disease susceptibility. Here, we investigated whether exposure to different microbes could influence zebrafish larval microbiota composition and modulate their immune response towards a saponin challenge. Adult zebrafish were exposed to the antibiotic oxytetracycline (OxyT) or control tank water and their intestinal content was harvested after 30 ​h (24 ​h exposure, 6 ​h wash-out). Subsequently, zebrafish embryos were exposed to either OxyT-treated content or non-treated content from 3 to 6 days post fertilization (dpf). At 6 dpf part of the group received a saponin challenge until 8 dpf. Zebrafish larvae exposed to OxyT-treated adult gut content (3–6 dpf) showed an altered microbiota composition compared to controls. Interestingly, larvae exposed to saponin-treated OxyT-content showed fewer macrophages (as visualized by fluorescent microscopy using mpx:GFP¹¹⁴;mpeg:mCherry transgenic fish) in the overall fish as well as around the gut area than saponin-treated control-exposed larvae. Fewer macrophages were associated with a decreased expression of interleukin 22 (<em>il22</em>) in larvae exposed to saponin-treated OxyT-content compared to controls. Overall, this study shows that exposure to different microbial environments early in life might affect disease susceptibility of larval zebrafish.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 3","pages":"Article 100187"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Invasive cichlids (Teleostei: Cichliformes) in the Amacuzac River, Mexico: Implications for the behavioral ecology of the native Mexican mojarra Amphilophus istlanus","authors":"M. Franco ,&nbsp;E. Arce ,&nbsp;N. Mercado-Silva ,&nbsp;A. Córdoba-Aguilar ,&nbsp;R. Ramírez-Rodríguez","doi":"10.1016/j.watbs.2023.100182","DOIUrl":"https://doi.org/10.1016/j.watbs.2023.100182","url":null,"abstract":"<div><p>The introduction of species is a leading cause of biodiversity loss and habitat alteration. Several species have invaded Mexican freshwater ecosystems, adversely affecting native fishes. Specifically, in the Amacuzac River of the Balsas Basin (Central Mexico), at least four non-native cichlids have been recorded: tilapia <em>Oreochromis</em> sp., convict cichlid <em>Amatitlania nigrofasciata</em>, spotcheek cichlid <em>Thorichthys maculipinnis</em>, and green terror <em>Andinoacara rivulatus</em>. These species co-occur with the Mexican mojarra <em>Amphilophus istlanus</em>, the only native cichlid in the Basin. Invasive cichlids compete for resources such as shelter and food, and prey on offspring of the Mexican mojarra. In the presence of invasive cichlids, the costs and risks of foraging and finding available shelter increase for the native cichlid. Predation pressure from invasive cichlid fishes on the Mexican mojarra increases the time invested by parents in caring for offspring and their stress levels, potentially contributing to Mexican mojarra population declines. Here, we gathered the best evidence available to discuss some mechanisms and consequences of such co-occurrences in relation to reproductive behaviors, parental care, competition for resources, and dominance hierarchy, and how the four invasive cichlids have negatively affected the Mexican mojarra.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 3","pages":"Article 100182"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physical habitat condition as a key tool to maintain freshwater biodiversity in neotropical artificial ponds","authors":"Marden S. Linares ,&nbsp;Livia B. dos Santos ,&nbsp;Marcos Callisto ,&nbsp;Jean C. Santos","doi":"10.1016/j.watbs.2023.100189","DOIUrl":"https://doi.org/10.1016/j.watbs.2023.100189","url":null,"abstract":"<div><p>In areas highly affected by anthropogenic disturbances, artificial (human-made) freshwater ecosystems can provide habitat for maintaining and conserving regional freshwater biodiversity. We assessed how the physical habitat of artificial ponds affected the structure of benthic macroinvertebrate assemblages. To do so, we tested two hypotheses. (1) Physical habitat disturbances are not detrimental to the diversity of nearby artificial ponds, and (2) Physical habitat disturbances do not cause significant shifts in taxonomic composition. Our results rejected both null hypotheses, i.e., macroinvertebrate diversity metrics correlated significantly and positively with Physical Habitat Integrity index scores, and only sites with high habitat condition scores were significantly associated with sensitive indicator taxa. Our results highlight the importance of maintaining physical habitat conditions for sustaining the ecological health of artificial ponds.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 3","pages":"Article 100189"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tropical and subtropical streams: A synthesis","authors":"Robert M. Hughes,&nbsp;Paulo Santos Pompeu,&nbsp;Marcos Callisto,&nbsp;Kai Chen,&nbsp;Leandro Juen,&nbsp;Bianca de Freitas Terra","doi":"10.1016/j.watbs.2023.100188","DOIUrl":"https://doi.org/10.1016/j.watbs.2023.100188","url":null,"abstract":"<div><p>Tropical and sub-tropical streams harbor great biodiversity and are responsible for crucial ecosystem services. Intending to contribute to the understanding of how anthropogenic stressors and pressures alter functional or taxonomic diversity in biotic assemblages or processes in these systems, <em>Water Biology and Security</em> published eight papers. The research originated from Cameroon (Central Africa), southeastern Brazil, eastern Amazonia, and eastern China, representing eight river basins: Huai, Sui and Yishusi from east Asia; São Francisco, Doce, Jequitinhonha, and Amazon from South America; and Congo from Africa. The last two are the most biodiverse on the planet. The studied biomes included tropical rainforest and savanna, neotropical savanna and rainforest, neotropical savanna mountains, and subtropical monsoon forest. The response variables included fish assemblages (3 papers), entire macroinvertebrate assemblages or sub-groups (4 papers), and stream physical habitat structure (1 paper).</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 3","pages":"Article 100188"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successful transplantation of cryopreserved spermatogonia in Sebastes schlegelii: A simple and suitable alternative approach for conservation of viviparous fish","authors":"Li Zhou ,&nbsp;Xueying Wang ,&nbsp;Shuran Du ,&nbsp;Shihong Xu ,&nbsp;Yanfeng Wang ,&nbsp;Zongcheng Song ,&nbsp;Qinghua Liu ,&nbsp;Jun Li","doi":"10.1016/j.watbs.2023.100142","DOIUrl":"https://doi.org/10.1016/j.watbs.2023.100142","url":null,"abstract":"<div><p>Black rockfish (<em>Sebastes schlegelii</em>) is one of the most important marine economic viviparous fishes. Recently, germplasm degradation and genetic diversity reduction have occurred due to overfishing and long-term artificial breeding. Germ cell transplantation combined with cryopreservation may be an alternative way to protect genetic resources. However, in viviparous fish that undertake fertilization and embryo development <em>in vivo</em>, transplantation is more difficult than in oviparous fish, including selection of transplantation stage, isolation of germ stem cells, and preparation of sterile recipients. This seriously restricts the development of viviparous transplantation. Therefore, in this study, we aimed to explore a transplantation method suitable for these species. Donor cells were isolated from cryopreserved whole testes of 300–400 ​g male <em>Sebastes schlegelii</em> in May, labeled by PKH26, and intra-peritoneally transplanted into allogeneic larvae at 5–10 days post-birth. Subsequently, the development of donor-derived cells in recipients were continuously detected by fluorescence labeling, histology, microsatellite markers, and fecundity tests. The results showed that donors were rich in spermatogonia (75%) and recipients maintained a high survival rate after transplantation, with a rate of &gt;20% at sexual maturity. Further, donor-derived cells successfully migrated (100%), colonized, and incorporated into the developing recipient gonad (93.33%). Finally, transplanted recipients could normally develop and differentiate into male and female individuals, with donor-derived gametes found in 65.38% of mature recipients. In the present study, we first establish a simple and suitable transplantation method for <em>Sebastes schlegelii</em> using immature males and specific larvae, which will serve as a promising tool in the protection of germplasm resources for this transplantation-restricted marine viviparous species.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 3","pages":"Article 100142"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal occurrence of microplastics in sediment of two South African recreational reservoirs","authors":"Thendo Mutshekwa ,&nbsp;Linton F. Munyai ,&nbsp;Lutendo Mugwedi ,&nbsp;Ross N. Cuthbert ,&nbsp;Farai Dondofema ,&nbsp;Tatenda Dalu","doi":"10.1016/j.watbs.2023.100185","DOIUrl":"https://doi.org/10.1016/j.watbs.2023.100185","url":null,"abstract":"<div><p>Inland water systems are regarded as a pathway and sink of plastic pollutants from the terrestrial environment. Aquatic ecosystems are globally contaminated with microplastics, but the spatiotemporal occurrence and density of microplastics in freshwater ecosystems remain poorly understood. The present study seasonally assessed differences in microplastic density in the sediments from two South African recreational reservoirs associated with low human activities (macadamia orchards) and high human activities (communal areas). Microplastics were recovered from all of the reservoirs assessed, indicating their extensive occurrence and densities. Microplastic numbers were significantly higher in reservoirs associated with high anthropogenic activities during the hot-dry season (140.6 particles kg⁻¹ dwt) and lower in reservoirs associated with low anthropogenic activities during the hot-wet and cool-dry seasons, i.e., 22.60 particles kg⁻¹ dwt and 16.13 particles kg⁻¹ dwt, respectively. Overall, polypropylene (31%) and polystyrene (30%) were identified as the dominant types of microplastic polymer in both reservoir types. Moreover, no correlative relationships were observed for environmental parameters and microplastic densities across reservoirs and seasons, suggesting a widespread and largely context-independent pollution level. These results show that microplastics are not evenly distributed between waters associated with low human activities and high human activities. Future work should seek greater sample sizes and centre around observing microplastic contamination in the region by examining their sources, transport, and impacts to freshwater environments, whilst informing management strategies.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 3","pages":"Article 100185"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of driving factors of algal growth in the South-to-North Water Diversion Project by Transformer-based deep learning","authors":"Jing Qian ,&nbsp;Nan Pu ,&nbsp;Li Qian ,&nbsp;Xiaobai Xue ,&nbsp;Yonghong Bi ,&nbsp;Stefan Norra","doi":"10.1016/j.watbs.2023.100184","DOIUrl":"https://doi.org/10.1016/j.watbs.2023.100184","url":null,"abstract":"<div><p>Accurate and credible identification of the drivers of algal growth is essential for sustainable utilization and scientific management of freshwater. In this study, we developed a deep learning-based Transformer model, named Bloomformer-1, for end-to-end identification of the drivers of algal growth without the needing extensive a priori knowledge or prior experiments. The Middle Route of the South-to-North Water Diversion Project (MRP) was used as the study site to demonstrate that Bloomformer-1 exhibited more robust performance (with the highest <em>R</em>², 0.80 to 0.94, and the lowest RMSE, 0.22–0.43 ​μg/L) compared to four widely used traditional machine learning models, namely extra trees regression (ETR), gradient boosting regression tree (GBRT), support vector regression (SVR), and multiple linear regression (MLR). In addition, Bloomformer-1 had higher interpretability (including higher transferability and understandability) than the four traditional machine learning models, which meant that it was trustworthy and the results could be directly applied to real scenarios. Finally, it was determined that total phosphorus (TP) was the most important driver for the MRP, especially in Henan section of the canal, although total nitrogen (TN) had the highest effect on algal growth in the Hebei section. Based on these results, phosphorus loading controlling in the whole MRP was proposed as an algal control strategy.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 3","pages":"Article 100184"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49733803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of hybrid constructed wetlands for phosphorus chemical industry tailwater treatment in the middle Yangtze river basin: Responses of plant growth and root-associated microbial communities","authors":"Qianzheng Li ,&nbsp;Yao Guo ,&nbsp;Junqi Yu ,&nbsp;Lu Yao ,&nbsp;Shun Liu ,&nbsp;Yahua Li ,&nbsp;Disong Chen ,&nbsp;Fei Peng ,&nbsp;Dong Xu ,&nbsp;Zhenbin Wu ,&nbsp;Qiaohong Zhou","doi":"10.1016/j.watbs.2023.100144","DOIUrl":"https://doi.org/10.1016/j.watbs.2023.100144","url":null,"abstract":"<div><p>Constructed wetlands are commonly utilized to treat industrial wastewater due to their effectiveness, affordability, and environmental benefits. Many phosphorus chemical enterprises in the middle Yangtze River basin are facing high pollution load challenges and efforts are needed to improve removal efficiency of pollutants. This work was the first to use constructed wetlands for phosphorus chemical industry tailwater (PCITW) treatment. The new hybrid constructed wetlands (CWs) proposed were the aeration horizontal subsurface flow constructed wetland (AHSCW) and the integrated vertical flow constructed wetland (IVCW), which were constructed on a pilot scale. Here, the effectiveness of pollutant removal along hybrid CWs, wetland plant growth, and root-associated microbial community responses to the PCITW were investigated. The results showed that there was spatial variation in removal of carbon, nitrogen, and phosphorus in the hybrid CWs, and that the AHSCW and the IVCW played synergistic roles in the removal of pollutants. Compared with influent, the toxic effect of effluent to embryos of a rare minnow (<em>Gobiocypris rarus</em>) was alleviated. Exposure to the effluent resulted in no malformation or death for embryos. Plants and microorganisms in the wetland system of the phosphate chemical tailwater were then compared with the control system of municipal tailwater. Plants in the former system had lower root density, and higher average root diameter, root shoot ratio, specific root length, and specific surface area. Plant roots also had decreased NH₄⁺ uptake ability but increased Ca²⁺ uptake to adapt to the high load and complex pollution stress. Unlike the control system, stochastic mechanisms had a more important role than deterministic processes in shaping the microbial community assembly associated with the PCITW. Meanwhile, analysis of microbial network-level topological characteristics demonstrated substantial reduction in network interactions complexity and microbiome stability in the treatment system. Findings from this study suggest wetlands will be helpful for efficient purification of phosphorus chemical industry wastewater in the Yangtze River basin.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 3","pages":"Article 100144"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Finding middle ground: Flow regimes designed for salmon and energy value","authors":"Henriette I. Jager,&nbsp;Rocio Uria-Martinez","doi":"10.1016/j.watbs.2023.100183","DOIUrl":"https://doi.org/10.1016/j.watbs.2023.100183","url":null,"abstract":"<div><p>In regulated rivers, shaping seasonal flows to recover species at risk depends on understanding when to expect conflicts with competing water users and when their interests are aligned. Multi-objective optimization can be used to reveal such conflicts and commonalities. When species are involved, multi-objective optimization is challenged by the need to simulate complex species responses to flow regimes. Previously, we addressed that challenge by developing a simplified salmon model (<em>Quantus</em>) that defines cohorts of salmon by the river section and time in which they were spawned. Salmon in these space-time cohorts are tracked from the time redds (nests) are constructed until the cohort exits the tributary <em>en route</em> to the ocean. In this study, we modeled seasonal patterns in energy value and developed a Pareto-optimal frontier of seasonal flow patterns to maximize in-river salmon survival and hydropower value. Candidate flow regimes were characterized by two pulse flows varying in magnitude, timing, and duration and constrained by a total annual flow near the historical median. Our analysis revealed times when economic and salmon objectives were aligned and times when they differed. Pulse flows that favored higher energy value were timed to meet demand during extreme temperatures. Both salmon and hydropower objectives produced optimal flow regimes with pulse flows in early summer, but only solutions favoring hydropower value included high flows in mid-winter. Solutions favoring higher age-0 salmon survival provided an extended pulse flow in late winter/early spring, which suggests that access to productive floodplain habitat allowed faster growth and earlier out-migration and reduced the need for higher temperature-moderating flows later in spring. Minimum flows were also higher among solutions favoring salmon over energy. The tools used to produce these results can help to design simplified seasonal flow regimes by revealing compromise solutions that satisfy both fish and energy producers and highlighting when potential conflicts are likely.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 3","pages":"Article 100183"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sperm whales in the waters off China: A glimpse of the past and present","authors":"Mingming Liu ,&nbsp;Mingli Lin ,&nbsp;Songhai Li","doi":"10.1016/j.watbs.2022.100133","DOIUrl":"https://doi.org/10.1016/j.watbs.2022.100133","url":null,"abstract":"<div><p>Spatiotemporal information is crucial for cetacean research and conservation, particularly for wide-ranging and migratory species. Sperm whales (<em>Physeter macrocephalus</em>) are distributed worldwide in deep oceans; however, little is known about the species in the western margin of the Pacific Ocean. Here, we examined the available information related to the occurrence, distribution, and potential habitats of sperm whales in the waters off China. Historical whaling information (18th–20th century) indicates that sperm whales have been captured in the East China Sea (ECS) and South China Sea (SCS). Furthermore, sporadic strandings have been recorded since the 1910s, and more frequently from 1990 onwards. Since 1990, accidental sightings have been documented in the eastern ECS, northeastern and southern SCS, and their adjacent waters. More recently (2019–2022), field encounters have been reported in dedicated ship-based investigations in the Xisha and Nansha waters, providing robust evidence of the regular existence and potential residency of sperm whales in the northwestern and central areas of the SCS. Female nursery groups suggest that the Xisha waters might be an important nursing ground for sperm whales. Satellite tracking data from four adults showed that sperm whales in the SCS might display both fine-scale and long-distance movements. Taken together, it appears that sperm whales are currently active in the waters off China, and that deep waters (depth &gt;200 ​m) in the ECS and SCS may provide the species with critical habitats. This review provides crucial baseline information on sperm whales in the waters off China, which may help to facilitate future research efforts and conservation initiatives for the species at national and cross-regional scales. More field investigations and other monitoring approaches including acoustic monitoring, biologging, photo-identification, and genetics are required to reveal the distribution, movement, and habitat use patterns of sperm whales in these waters.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 3","pages":"Article 100133"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49734119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}