Water Biology and Security最新文献

{"title":"The immunoregulatory role of fish specific type II SOCS via inhibiting metaflammation in the gut-liver axis","authors":"Junwei Shan ,&nbsp;Guangxin Wang ,&nbsp;Heng Li ,&nbsp;Xuyang Zhao ,&nbsp;Weidong Ye ,&nbsp;Lian Su ,&nbsp;Qingsong Zhu ,&nbsp;Yuhang Liu ,&nbsp;Yingyin Cheng ,&nbsp;Wanting Zhang ,&nbsp;Nan Wu ,&nbsp;Xiao-Qin Xia","doi":"10.1016/j.watbs.2022.100131","DOIUrl":"https://doi.org/10.1016/j.watbs.2022.100131","url":null,"abstract":"<div><p>SOCS8, also known as CISHb, is a fish-specific type II SOCS. Because CISH binds to cytokine receptors and may inhibit STAT5 activation (a substrate of the insulin receptor), SOCS8 may be involved in the control of metaflammation. The <em>socs8−/−</em> zebrafish were created, and both longer trunks and intestinal villi were observed in 1-month-old (mo) fish. Altered mucosal immunity and gut-liver metabolism were also found in <em>socs8−/−</em> fish. Increased intestinal neutrophils and macrophages, together with overexpression of cytokines and T cell markers in this mutant fish, suggested SOCS8's immunoregulating role. During modeling of soybean-induced enteritis using the 3 ​mo zebrafish, lower expression levels of inflammatory genes but more mucosa barrier disruption were discovered in <em>socs8−/−</em> zebrafish, compared with wide type counterparts<em>.</em> Furthermore, the shrunk villi at 6 ​mo in <em>socs8−/−</em> fish suggested that the mucosa might have been protected by SOCS8. This is also consistent with the assertion that metaflammation eventually leads to tissue degeneration and premature death. The fact that <em>socs8−/−</em> fish had more hepatic oil droplets compared to their wild-type counterparts suggested SOCS8's role in inhibiting hepatic metaflammation. Transcriptomic analysis as well as 16S rRNA gene sequencing were done on 3 ​mo <em>socs8−/−</em> fish to methodically reveal the altered immunity and metabolic reprogramming in the gut and liver caused by <em>socs8−/−</em>. The enriched GO terms for the intestinal tract, such as \"cytokine-mediated signaling pathway\" and \"response to external biotic stimulus\", as well as KEGG pathways in both gut and liver like \"carbon metabolism\" and \"glycolysis/gluconeogenesis\", were consistent with previously revealed pathological clues and improved growth performance at early age, respectively. In addition, the microbiota in the <em>socs8−/−</em> strain had adapted to the host's increased carbohydrate metabolism, as evidenced by higher levels of Bacteroidota. Furthermore, Verrucomicrobiota associated with immunoregulation were found in lower abundance in <em>socs8−/−</em> fish. As a result, current findings indicate that SOCS8 plays immunoregulatory and mucosa-protective roles in the fish gut and liver by inhibiting carbohydrate metabolism.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 2","pages":"Article 100131"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49734118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research advances in the structure, function, and regulation of the gill barrier in teleost fish","authors":"Xingyu Chen ,&nbsp;Shubin Liu ,&nbsp;Qianwen Ding ,&nbsp;Tsegay Teame ,&nbsp;Yalin Yang ,&nbsp;Chao Ran ,&nbsp;Zhen Zhang ,&nbsp;Zhigang Zhou","doi":"10.1016/j.watbs.2023.100139","DOIUrl":"https://doi.org/10.1016/j.watbs.2023.100139","url":null,"abstract":"<div><p>The gill is an important organ in fish that is involved in various physiological functions, including respiration, filter-feeding, ammonia nitrogen excretion, and osmoregulation. The physiological functions of gills are closely related to their structure, external feeding conditions, and environmental factors. Herein, we first briefly summarize the basic structure and functions of the gills of teleosts, then explain the structure and function of the gill barrier (as a microbial barrier, a chemical barrier, a physical barrier, and an immune barrier) in detail. Finally, we introduce the regulation of fish gill barrier health by ubiquitous feeding factors and environmental factors, and provide an outlook on the prospects of gill barrier health. We found that improving health through modulating feed factors is more convenient than improving environmental factors. This review increases our understanding of how nutrient composition and level in fish diet influences gill health and overall growth performance of fish.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 2","pages":"Article 100139"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A finTRIM member 100 (FTR100) is unique to Otomorpha fish for constitutive regulation of IFN response","authors":"Wen-Hao Guo ,&nbsp;Cheng Dan ,&nbsp;Xiu-Ying Gong ,&nbsp;Yi-Lin Li ,&nbsp;Hao-Yu Sun ,&nbsp;Zi-Ling Qu ,&nbsp;Li-Li An ,&nbsp;Xiang Zhao ,&nbsp;Jie Mei ,&nbsp;Jian-Fang Gui ,&nbsp;Yi-Bing Zhang","doi":"10.1016/j.watbs.2023.100137","DOIUrl":"https://doi.org/10.1016/j.watbs.2023.100137","url":null,"abstract":"<div><p>Vertebrate interferon (IFN) expression is fine-tuned in order to avoid excessive tissue injury under normal conditions and during virus infection. FinTRIM (fish novel TRIM, FTR) proteins are reported to regulate the fish IFN response. Here, we identify a novel finTRIM gene from yellow catfish (<em>Pelteobagrus fulvidraco</em>), which is sequentially named PfFTR100 according to the nomenclature rule in zebrafish. Genome-wide analyses reveal that FTR100 is unique to Otomorpha fish, with a single copy in spite of additional genome duplication in some fish species. Considering that few of the 99 finTRIM genes identified in zebrafish are conserved in main fish branches and most, such as FTR100, are unique to distinct branches due to lineage-specific expansion of finTRIM genes, we develop a nomenclature for newly cloned finTRIM genes from different fish species. PfFTR100 mRNA is not induced by virus infection, with a relatively high expression level comparable to that of cellular IFN and some IFN-stimulated genes (ISGs) in virally-infected tissues. However, ectopically-expressed PfFTR100 protein is attenuated in virally-infected cells through the proteasomal-dependent pathway. Overexpression of PfFTR100 promotes SVCV replication by downregulating the constitutive and inducible IFN response via a mechanism by which PfFTR100 targets IRF3 and IRF7 to attenuate their mRNA levels rather than their protein levels. Our results indicate that yellow catfish FTR100 is essential for homeostatic regulation of fish tonic IFN response.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 2","pages":"Article 100137"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Initial discovery of microplastic pollution in Mnemiopsis leidyi (Ctenophora: Lobata)","authors":"Mariana Beatriz Paz Otegui ,&nbsp;Gabriela Carvalho Zamprogno ,&nbsp;Enrique Ronald Yapuchura Ocaris ,&nbsp;Mercia Barcellos da Costa","doi":"10.1016/j.watbs.2023.100140","DOIUrl":"https://doi.org/10.1016/j.watbs.2023.100140","url":null,"abstract":"<div><p>The production of plastics has exponentially increased over time such that the observation of microplastics in the oceans has become a major concern given how frequently these particles and marine biota may interact. Microplastics can be ingested by diverse species, including invertebrates, which can lead to physical damage due to their small size and potentially chemical damage related to the ingestion of monomers, polymeric additives, or adsorbed chemicals. This study reports on the first evidence of microplastics in <em>Mnemiopsis leidyi</em>. Microplastics were described by type, quantified, and had their chemical composition identified. In total, 328 samples of microplastic (MP) candidates were observed: blue fibers were the most abundant, followed by black, red, and green fibers as well as blue fragments. Three types of microplastics were chemically identified as polyurethane, unplasticized polyvinylchloride, and polyethylene polylauryllactam. Overall, this study demonstrated that <em>Mnemiopsis leidyi</em> have the capacity to incorporate microplastics. Although more studies are needed, <em>Mnemiopsis leidyi</em> is relatively abundant on the Atlantic coast and should be recommended for use as a bioindicator for microplastics.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 2","pages":"Article 100140"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An overview of research efforts to understand the effects of underwater sound on cetaceans","authors":"Shane Guan ,&nbsp;Tiffini Brookens","doi":"10.1016/j.watbs.2023.100141","DOIUrl":"https://doi.org/10.1016/j.watbs.2023.100141","url":null,"abstract":"<div><p>Cetaceans are aquatic mammals living in an environment that is more suited for hearing than vision. As such, their sensory systems largely utilize acoustic cues for navigation, communication, foraging, and predator avoidance. However, the elevation of underwater sound levels from increased human activities has adversely affected cetaceans’ use of sound to perform vital life functions. To address those impacts, scientific studies have been conducted to understand the behavioral, psychoacoustical, physiological, and physical responses by cetaceans that have been exposed to anthropogenic sound. These studies range from captive experiments involving auditory thresholds and noise-induced threshold shifts, to field observations of behavioral disturbance from sound exposure, to post-mortem examinations of physical manifestations in stranded animals. Over the years, results from these studies have assisted regulatory agencies in developing a series of criteria and thresholds for cetacean conservation and management around the world. This paper provides a high-level overview of worldwide research efforts that have been dedicated to understanding the effects of underwater sound on cetaceans. The review is not intended to be exhaustive but rather to capture major efforts and significant findings in this field. In addition, the review excludes synthesis studies and modeling exercises that do not involve direct research on target species.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 2","pages":"Article 100141"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49726704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mine tailings storage dams modify upstream headwater fish assemblages","authors":"Gilberto N. Salvador ,&nbsp;Robert M. Hughes ,&nbsp;Fábio Vieira ,&nbsp;Raphael Ligeiro ,&nbsp;Luciano F.A. Montag","doi":"10.1016/j.watbs.2023.100136","DOIUrl":"https://doi.org/10.1016/j.watbs.2023.100136","url":null,"abstract":"<div><p>Riverscape connectivity is a critically important component determining the ecological condition of lotic ecosystems. We evaluated changes in fish assemblages caused by the loss of connectivity by mine tailings storage dams (TSDs), hypothesizing that headwater fish assemblages are restructured by TSDs located downstream, even though the upstream habitats are not altered. We used standard methods to collect fish in 24 first to third order sites, with half draining to TSDs (dammed) and the other half free from this impact (undammed). To identify differences between treatments, we used PERMANOVA to test both environmental variables and ichthyofauna composition (Bray-Curtis similarity index) and evaluated the biological metrics that most influenced assemblage composition change. As expected, we observed no difference between treatments for environmental variables, but we did observe differences in fish assemblage composition. We also observed five metrics with lower values in dammed streams (richness and abundance of intolerant species, siluriform richness, and abundance of <em>Pareiorhaphis</em> cf. <em>proskynita</em> and <em>Trichomycterus brasiliensis</em>) and two metrics with higher values in dammed streams (perciform richness and abundance of <em>Oligosarcus argenteus</em>). We believe these changes resulted from of stream fragmentation by TSDs, in addition to source-sink mechanisms and conclude that mine TSDs located downstream change headwater fish assemblages, an impact often neglected in biomonitoring and bioassessment studies.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 2","pages":"Article 100136"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fish herpesvirus KLP manipulates Beclin1 to selectively degrade MITA through a precise autophagic manner for immune evasion","authors":"Long-Feng Lu ,&nbsp;Zhuo-Cong Li ,&nbsp;Xiao-Yu Zhou ,&nbsp;Can Zhang ,&nbsp;Cheng-Yan Mou ,&nbsp;Xue-Li Wang ,&nbsp;Dan-Dan Chen ,&nbsp;Li Zhou ,&nbsp;Jian-Fang Gui ,&nbsp;Shun Li","doi":"10.1016/j.watbs.2022.100092","DOIUrl":"https://doi.org/10.1016/j.watbs.2022.100092","url":null,"abstract":"<div><p>Control of host autophagy acceleration or attenuation has been confirmed in multiple terrestrial animal viruses. Little is known about such mechanisms in aquatic viruses. Here, we report a selective and ingenious autophagy modulation regulated by kinase-like protein (KLP) of cyprinid herpesvirus 2 (CyHV2) to restrict interferon (IFN) production by degrading IFN regulatory factor (IRF) 3 activation (MITA). First, exogenous DNA and RNA-mediated IFN activation were both abrogated by CyHV2 KLP. The common intersection point of MITA with these two signaling pathways was the interaction with KLP. The C terminus of MITA was indispensable for the interaction and was recruited by KLP in subcellular colocalization analysis. Subsequently, we found that KLP degraded MITA in an autophagy-lysosome-dependent manner and, interestingly, individual KLP could not launch host autophagic flow except in the presence of MITA. KLP was also colocalized with the autophagy components Beclin1 and ATG14 and enhanced Beclin1 stability, but not ATG14, through K63-linked polyubiquitination. Finally, KLP significantly decreased the normal state or MITA-enhanced cellular antiviral capacity. These data demonstrated an elaborate autophagic process manipulated by a fish virus only in the presence of the host target, illuminating a mechanism of aquatic viral immune evasion.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 1","pages":"Article 100092"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species-level monitoring of rare and invasive fishes using eDNA metabarcoding in the middle and upper Yarlung Zangbo River, Tibet","authors":"Xiu Feng ,&nbsp;Bing Li ,&nbsp;Yifeng Chen ,&nbsp;Ren Zhu ,&nbsp;Yintao Jia ,&nbsp;Xiaoyun Sui","doi":"10.1016/j.watbs.2022.100089","DOIUrl":"https://doi.org/10.1016/j.watbs.2022.100089","url":null,"abstract":"<div><p>Fish diversity of the Yarlung Zangbo River is very sensitive and vulnerable to biological invasion, anthropogenic activities and climate change, especially in the upper and middle reaches where several endemic fishes have become endangered and nearly ten invasive fishes have been established. Here, we used environmental DNA (eDNA) metabarcoding to monitor rare and invasive fishes, and to assess diversity in 25 sites from two wetlands (Lalu and Chabalang) and the main channel (YT), within the upper and middle reaches. To obtain a species-level resolution, we evaluated species discrimination potentials of three mitochondrial markers and found Cytb had the highest average genetic distance at each taxonomic level followed by COI and 12S. The 12S was unqualified for species assignment, as two species shared identical haplotypes. The newly designed Cytb primers used for metabarcoding showed an average mismatch of 0.28 and amplified well across species. In total, 8942 operational taxonomic units (OTUs) were obtained based on a 100% identity threshold, among which 98.1% were assigned to 24 fishes based on our custom-made database and the remaining were assigned to six fishes based on the NCBI nt database. Almost all captured fishes were detected by the eDNA method except for two species. However, 12 fishes detected by the eDNA method were not listed in catch data for several sites, including one endangered species (<em>Oxygymnocypris stewartii</em>), four previously recorded non-native species and two unrecorded non-native species (<em>Monopterus albus</em> and <em>Siniperca chuatsi</em>). The alpha diversities estimated by eDNA and capture-based methods were correlated for sites at Lalu. Both methods revealed significant differences in community composition between YT and the wetlands. Our results provide both basic information for conservation and management of rare and invasive fishes in the Yarlung Zangbo River and a framework of fish eDNA metabarcoding with a species-level resolution.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 1","pages":"Article 100089"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of single-cell sequencing to reveal stem/progenitor cells in animal organ regeneration","authors":"Suxiang Lu ,&nbsp;Igor Schneider ,&nbsp;Honghui Zeng ,&nbsp;Shunping He","doi":"10.1016/j.watbs.2022.100081","DOIUrl":"https://doi.org/10.1016/j.watbs.2022.100081","url":null,"abstract":"<div><p>Advances in single-cell sequencing technologies have made it possible to reveal the cellular basis of complex organ regeneration processes. In this review, we briefly introduce commonly used high-throughput single-cell sequencing platforms and their characteristics. We discuss in depth how single-cell sequencing techniques can be used in the study of regeneration to reveal stem/progenitor cell types and their developmental trajectories. From the perspective of stem cells, we review animal models of regenerative biology and organ models involved in regenerative medicine, with a focus on limb/fin/digit-tip regeneration. Finally, we discuss previous prospective work on improving systematic approaches to explore the origin and evolution of cellular diversity, as well as future research directions.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 1","pages":"Article 100081"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49712008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural variation of physical-habitat conditions among least-disturbed streams of a neotropical river basin in Brazil","authors":"Yuri Malta Caldeira ,&nbsp;Ruanny Casarim ,&nbsp;Paulo Santos Pompeu","doi":"10.1016/j.watbs.2022.100091","DOIUrl":"https://doi.org/10.1016/j.watbs.2022.100091","url":null,"abstract":"<div><p>The determination of natural variation in physical habitat conditions and the establishment of least-disturbed reference standards is important for ecosystem conservation. Tropical regions hold most of the global freshwater diversity, nevertheless little is known about the natural conditions of their physical habitats. We calculated 255 physical habitat metrics for 31 stream sites in five protected areas of the Brazilian São Francisco River basin to identify the most variable characteristics among tropical streams in least-disturbed areas. We performed principal components analyses (PCA) to find the most relevant metrics for ordination of streams in the following categories: water quality (2); substrate (7); fish shelter (6); riparian vegetation (9); and channel morphology (7). We used distance-based linear models (DISTLM) to test how much of the variation of these metrics could be explained by geographic position, average elevation, and average discharge of stream sites. The best statistically significant models explained 42% of substrate (33% geographic position; 9% average elevation), 26% of channel morphology (22% geographic position; 4% average discharge), 23% of water quality (14% geographic position; 9% average discharge), 17% of fish shelter (9% geographic position; 8% average elevation), and 14% of riparian vegetation (7% geographic position; 7% average elevation) variation. Thus, regional features (e.g. geology and climate) related to the geographic position are of greatest importance, followed by average elevation and discharge for determining physical habitat characteristics. Therefore, we suggest that reference sites should be set for tropical streams in the same regional landscape and with similar average elevation and discharge.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 1","pages":"Article 100091"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49733643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}