Water Biology and Security最新文献

{"title":"Condition index for Cerrado springs: A simplified and efficient tool for conservation planning","authors":"Rafaella Oliveira Martins ,&nbsp;Cristhiane Vasconcelos ,&nbsp;Kaiqui da Silva Rodrigues ,&nbsp;Ully Mattilde Pozzobom ,&nbsp;Leandro Schlemmer Brasil","doi":"10.1016/j.watbs.2024.100336","DOIUrl":"10.1016/j.watbs.2024.100336","url":null,"abstract":"<div><div>Springs are essential for water security in the Cerrado (Neotropical Savanna Biome), especially considering its prolonged dry periods. However, the protection of these environments is often compromised by various human activities, necessitating the creation of plans for protecting and rehabilitating already degraded springs. Thus, assessing the environmental condition of springs is essential for adopting effective protection or rehabilitation measures. Therefore, the aim of this study is to apply protocols for assessing spring environmental conditions in the Cerrado. To achieve this, we conducted a literature review of existing environmental protocol models, adapted them after considering Cerrado environmental conditions, and field-tested a protocol termed the Spring Condition Index (SCI). We used linear models to measure the relationship between SCI scores and physical-chemical variables of water and local vegetation. We assessed a total of 41 springs, and SCI values ranged from 0.61 to 0.94, where values closer to 1 indicated a more preserved spring. SCI showed a positive linear relationship with dissolved oxygen and landscape vegetation cover, and a negative linear relationship with water temperature, pH, and electrical conductivity. Despite a macroscopic analysis, these results indicate that SCI is effective and robust for measuring spring environmental condition, with the advantage of being conducted in just a few minutes. Thus, we suggest its application throughout the biome so that springs with lower SCI values can be quickly identified and prioritized for rehabilitation in the Cerrado Biome.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 2","pages":"Article 100336"},"PeriodicalIF":5.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA profiling yields immune response and metabolic changes in juvenile largemouth bass (Micropterus salmoides) infected with LMBV","authors":"Xiaodan Liu ,&nbsp;Liwen Zhang ,&nbsp;Xu Tan ,&nbsp;Mengya Guo ,&nbsp;Weiguang Kong ,&nbsp;Zhenhua An","doi":"10.1016/j.watbs.2024.100327","DOIUrl":"10.1016/j.watbs.2024.100327","url":null,"abstract":"<div><div>The largemouth bass virus (LMBV) exhibits high pathogenicity in both adult and juvenile largemouth bass, causing substantial economic losses within the aquaculture industry. MicroRNAs (miRNAs) are crucial in controlling viral infections and the host's immune responses, making them significantly valuable in the treatment and diagnosis of diseases. Nevertheless, research on miRNA expression profiles associated with LMBV infection in largemouth bass is currently insufficient. This research attempts to investigate the roles and molecular mechanisms of miRNAs in the potential immune response and metabolic alterations triggered by LMBV infection in largemouth bass using miRNA sequencing. Following quality screening, the infection group and control group yielded a combined total of 142.73 million clean reads, with lengths predominantly at 22 ​nt. 1718 known miRNAs were identified, including 238 differentially expressed miRNAs (DEMs). In addition, 400 novel miRNAs were predicted, 36 of which were DEMs. To gain further insight into the immune and metabolic related biological functions of DEMs, target gene prediction was conducted. KEGG pathway analysis revealed that LMBV impacted pathways such as Endocytosis, Purine metabolism, Phosphatidylinositol, Fatty acid Biosynthesis, and Phagosome signaling systems, highlighting the vital role of miRNAs in immune responses and metabolic alterations. Furthermore, the miRNA-mRNA interaction network revealed crucial miRNAs and their corresponding target genes involved in conferring resistance against viral infections by utilizing metabolic and immune related pathways as the foundation. Ten DEMs were selected at random for real-time quantitative PCR (qRT-PCR), and results exhibited expression patterns that were consistent with sequencing data. These findings validate the immune and metabolic regulatory function of miRNAs against LMBV in largemouth bass, offering valuable perspectives for the prevention and management of illnesses linked to iridoviruses.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 2","pages":"Article 100327"},"PeriodicalIF":5.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of microplastics on larval ingestion, survival, and development of sea cucumber Holothuria leucospilota","authors":"Hong Wu ,&nbsp;Mohamed Mohsen ,&nbsp;Yikun Cen ,&nbsp;Yukai Yang ,&nbsp;Zonghe Yu","doi":"10.1016/j.watbs.2024.100329","DOIUrl":"10.1016/j.watbs.2024.100329","url":null,"abstract":"<div><div>The presence of microplastics in the marine environment poses a potential threat to juvenile and adult sea cucumbers; however, the effects of microplastics on the early developmental stages of sea cucumbers are poorly understood. In this study, the larvae of <em>Holothuria leucospilota</em> were exposed to polystyrene (PS) particles of varying sizes (1–60 ​μm) to investigate the influences of microplastics on food ingestion, digestion, and performance of larval <em>H. leucospilota</em> at different developmental stages. The fluorescence tracing experiment revealed that the early- and mid-auricularia were capable of ingesting particles up to 20 ​μm in size, whereas late-auricularia larvae could ingest particles up to 40 ​μm in size. It was observed that microplastic particles accumulated in the stomach of larvae over a certain period, making it difficult for them to eliminate. Microplastic particles of 10 ​μm could be observed aggregating in the stomach of larvae at all developmental stages. The 20 ​μm and 40 ​μm particles had limited effects on larval survival but caused developmental retardation. Larvae that were exposed to 10 ​μm and 60 ​μm particles had significantly lower survival rates compared to the control group. Moreover, microplastics had a negative effect on the growth of larvae, and larger particles (20 ​μm, 40 ​μm and 60 ​μm) had greater effects than smaller ones (1 ​μm, 5 ​μm and 10 ​μm). The presence of microplastics hindered the development and metamorphosis of larvae, in particular by inhibiting the maturation of their ciliary band and esophagus. In conclusion, microplastics have negative impacts on larval <em>H. leucospilota</em>, and therefore, effective measures should be taken to prevent the introduction of microplastics into hatcheries artificially breeding sea cucumbers.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 2","pages":"Article 100329"},"PeriodicalIF":5.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Licochalcone A alleviates the pathogenicity of Aeromonas hydrophila by affecting the function of aerolysin","authors":"Jing Dong ,&nbsp;Shengping Li ,&nbsp;Shun Zhou ,&nbsp;Yongtao Liu ,&nbsp;Qiuhong Yang ,&nbsp;Yibin Yang ,&nbsp;Ning Xu ,&nbsp;Xiaohui Ai ,&nbsp;Bo Cheng","doi":"10.1016/j.watbs.2024.100326","DOIUrl":"10.1016/j.watbs.2024.100326","url":null,"abstract":"<div><div>Proteins in aquatic products satisfy the increasing demand of high-quality proteins of humans, resulting in a rapid development of freshwater aquaculture. However, bacterial diseases in aquatic animals often cause economic losses worldwide. Antibiotics are frequently used in aquaculture to deal with bacterial infections, but antibiotic resistance has restricted its application. Therefore, it is necessary to develop drugs on account of novel strategies. Here, aerolysin, the main virulence factor of <em>Aeromonas hydrophila</em> (<em>A. hydrophila</em>), was defined as a target to develop anti-virulence drugs. The results showed that Licochalcone A (LCA) with little bacteriostatic activity could decrease the hemolytic activity of aerolysin without affecting its production. The binding mode and potential binding sites were determined following molecular docking and dynamic simulation. Ser33 and Pro365 were confirmed to be the main binding sites according to the results of fluorescence quenching assay with mutated aerolysin. The mechanism by which LCA inhibited aerolysin was hindering its pore-forming activity. Moreover, cell viability assays and animal studies demonstrated that LCA could provide a significant protection to human alveolar epithelial cells from aerolysin-mediated cell damage and decrease the pathogenicity of <em>A. hydrophila</em> to channel catfish. These findings suggested that LCA might be a novel candidate for treating <em>A. hydrophila</em> infections and partly determined the mechanism of herbal medicine in dealing with bacterial infections.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 2","pages":"Article 100326"},"PeriodicalIF":5.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the influence of offshore wind farm noise on the fishes and dolphins in the Pearl River Estuary","authors":"Zhongchang Song ,&nbsp;Weijie Fu ,&nbsp;Hongquan Li ,&nbsp;Yingnan Su ,&nbsp;Zhanyuan Gao ,&nbsp;Wenxin Fan ,&nbsp;Jiangang Hui ,&nbsp;Wenzhan Ou ,&nbsp;Shengyao Sun ,&nbsp;Teng Wang ,&nbsp;Honghui Huang ,&nbsp;Yu Zhang","doi":"10.1016/j.watbs.2024.100318","DOIUrl":"10.1016/j.watbs.2024.100318","url":null,"abstract":"<div><div>In this study, we recorded the noise radiating from a wind farm and evaluated its potential impact on the fishes and Indo-Pacific humpback dolphins (<em>Sousa chinensis</em>) residing in the Pearl River Estuary. The pile driving pulses, recorded 30 ​m from the construction site, had a mean zero-to-peak sound pressure level of 195.1 ​dB (re 1 ​μPa) (<em>n</em> ​= ​238), exceeding the hearing thresholds of both fishes and Indo-Pacific humpback dolphins. The operational noise from the wind farm was significantly lower in amplitude; therefore, our analysis primarily focused on the radiation of pile driving noise. Acoustic measurements taken at three different distances during pile driving events supported the development of an acoustic propagation model, which was then used to predict the sound exposure levels of pile driving pulses radiating from 5 ​m below the water surface. By referring to established noise exposure metrics, we estimated an impact zone of 12.8 ​m for fishes. For the Indo-Pacific humpback dolphins, the permanent and temporary threshold shift zones were predicted to be 32.4 ​m and 580.9 ​m, respectively. Our findings underscore the importance of increasing our understanding on hearing sensitivities of the local inhabitants in the Pearl River Estuary and identify their core habitats. This approach enabled us to use the model to estimate impact zones and better protect the local marine life from the effects of pile driving noise radiation.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 1","pages":"Article 100318"},"PeriodicalIF":5.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hot and cold exposure triggers distinct transcriptional and behavioral responses in laboratory-inbred pond snails","authors":"Veronica Rivi ,&nbsp;Anuradha Batabyal ,&nbsp;Cristina Benatti ,&nbsp;Fabio Tascedda ,&nbsp;Johanna Maria Catharina Blom ,&nbsp;Ken Lukowiak","doi":"10.1016/j.watbs.2024.100315","DOIUrl":"10.1016/j.watbs.2024.100315","url":null,"abstract":"<div><div>Animals exhibit remarkable behavioral and molecular adaptations to cope with thermal stressors, which are crucial for survival in variable environments that are exacerbated by climate change. Aquatic poikilotherms like our model organism—the pond snail <em>Lymnaea stagnalis</em>—face significant challenges due to their dependence on external temperatures. Our study provides valuable insights into the different behavioral and molecular responses of lab-inbred snails to cold and heat shock stressors (i.e., 4 ​°C and 30 ​°C), particularly in the context of learning and memory formation. We found that while short-term (1 ​h) cold exposure transiently upregulated the expression levels of HSP70 and HSP40 in the snail's central ring ganglia, prolonged cold exposure (24 ​h) resulted in a significant downregulation of LymMIPII and an upregulation of LymMIPR. These data suggest, albeit at the transcriptional level, the existence of a negative feedback loop necessary for sustaining cellular functions when metabolic demands might shift towards conserving energy during prolonged cold exposure. At the behavioral level, we found that, compared to heat shock, cold exposure did not result in a Garcia effect (i.e., a “special form” of conditioned taste aversion). The difference in memory outcomes was associated with changes in the expression levels of selected targets involved in neuronal plasticity and the stress response. While both cold and heat shock upregulated the HSP levels in the snail's central ring ganglia, cold exposure did not affect the expression levels of the neuroplasticity genes LymGRIN1 and LymCREB1, contrasting with heat shock's neurogenic effects. Overall, this study provides insights into <em>L</em>. <em>stagnalis</em>'s adaptive responses to thermal stressors, emphasizing different molecular strategies for coping with heat versus cold challenges in aquatic environments. These findings contribute to our understanding of thermal biology and stress physiology in aquatic organisms, underscoring the importance of molecular mechanisms in shaping species' resilience in dynamic environments.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 1","pages":"Article 100315"},"PeriodicalIF":5.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seahorse nanos3 plays essential roles in germ cell development in the absence of nanos2","authors":"Lingzhen Chen ,&nbsp;Lu Tang ,&nbsp;Qiang Lin ,&nbsp;Yanhong Zhang","doi":"10.1016/j.watbs.2024.100312","DOIUrl":"10.1016/j.watbs.2024.100312","url":null,"abstract":"<div><div>Seahorses are specialized teleosts due to their unique male pregnancy and reproductive physiology. In particular, they have distinct gonad structures and different patterns of gametogenesis development in both sexes. However, the molecular mechanism of germ cell development in seahorses remains unknown. <em>Nanos</em>, which encodes conserved zinc-finger RNA-binding proteins, play essential roles for germline development in both vertebrates and invertebrates. In this study, we characterized the <em>nanos</em> gene family members and their potential roles in germline development in the lined seahorse, <em>Hippocampus erectus</em>. We showed that the <em>nanos</em> family members in the seahorse are the <em>nanos1a</em>, <em>nanos1b</em>, and <em>nanos3</em> genes, while <em>nanos2</em>, a key germline stem cell (GSC) maintenance factor, is intriguingly absent. More interestingly, <em>nanos3</em>, which is reported to be a female-specific gene, was also highly expressed in male gonads, as well as in the early developmental stages of testes and ovaries. Our results also showed that <em>nanos3</em> was highly expressed in early meiotic germ cells, and that expression levels of <em>nanos3</em> were lower in the gonad after TBT exposure. These findings suggest that <em>nanos3</em> may function during male germ cell development, and in female initial germ cell production – processes which are regulated by <em>nanos2</em> in other teleosts. This is the first research into how <em>nanos</em> governs germ cell development in an ovoviviparous fish. Our study provides insights into the regulatory mechanisms of germ cells development, as well as the early events during gametogenesis in the family <em>Syngnathidae.</em></div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 1","pages":"Article 100312"},"PeriodicalIF":5.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143171580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Black carp RNF115 restricts IRF3/7-mediated antiviral signaling in innate immunity","authors":"Yixuan He ,&nbsp;Qun Wang ,&nbsp;Lili Xiao ,&nbsp;Hui Wu ,&nbsp;Jun Xiao ,&nbsp;Jun Zou ,&nbsp;Hao Feng","doi":"10.1016/j.watbs.2024.100310","DOIUrl":"10.1016/j.watbs.2024.100310","url":null,"abstract":"<div><div>The Really Interesting New Gene (RING) ubiquitin E3 ligase family comprises a large number of members and plays a crucial role in the antiviral process. RING finger protein 115 (RNF115), also known as BCA2, Rabring7, or ZNF364, is a novel RING domain protein. In this paper, we cloned the RNF115 homologue from black carp (<em>Mylopharyngodon piceus</em>) and characterized it. The open reading frame of black carp RNF115 contains 933 nucleotides and encodes 310 amino acids. The C-terminal RING domain of RNF115 is highly conserved among various homologous species. Immunofluorescence assays revealed the cytoplasmic and nuclear distribution of RNF115 in the presence or absence of spring viremia of carp virus (SVCV) infection. Overexpression of RNF115 impaired interferon (IFN) and the related interferon-stimulated gene (ISG) mRNA expression, while upregulating SVCV replication. <em>Ex vivo</em> knockdown of RNF115 offered the host cells enhanced antiviral signaling. <em>In vivo</em> knockdown of RNF115 also strengthened black carp's antiviral capacity. Additionally, the results of a dual-luciferase reporter assay, plaque assay, and qRT-PCR assay demonstrated that co-transfection of RNF115 with IRF3/7 reduced IRF3/7-induced IFN transcription and antiviral ability. The association between RNF115 and IRF3/7 was detected by co-immunoprecipitation and immunofluorescence assays. Co-transfection of RNF115 with IRF3/7 also reduced the protein levels of IRF3/7, which were rescued by MG132. The enhanced K48-linked ubiquitination of IRF3/7 under the condition of RNF115 co-transfection implied the ubiquitin/proteasome degradation pathway catalyzed by RNF115. Cysteine 238 and 241 in the RING domain are the main enzyme active sites for RNF115, and the mutant C238/241A lost most of its ability to restrict IRF3/7. In conclusion, black carp RNF115 dampens IRF3/7-mediated IFN signaling through facilitating the ubiquitination and degradation of IRF3/7, which sheds light on the regulation of IFN signaling.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 1","pages":"Article 100310"},"PeriodicalIF":5.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Muscle metabolism in response to oxidized fish oil feed in juvenile Nile tilapia","authors":"Xinyuan Li ,&nbsp;Lili Chen ,&nbsp;Hua Wen ,&nbsp;Xuan Wang ,&nbsp;Di Peng ,&nbsp;Jianmin Zhang ,&nbsp;Yangyang Liu ,&nbsp;Ming Jiang ,&nbsp;Lixue Dong ,&nbsp;Feng Huang ,&nbsp;Juan Tian","doi":"10.1016/j.watbs.2024.100321","DOIUrl":"10.1016/j.watbs.2024.100321","url":null,"abstract":"<div><div>To investigate the effect of oxidized fish oil on fish muscle metabolic responses and flesh quality, Nile tilapia (<em>Oreochromis niloticus</em>) weighing 13.73 ​± ​0.31 ​g were fed two diets for 12 weeks: a fresh fish oil (FFO) and a highly oxidized fish oil (OFO) diet. The peroxide value of the FFO and OFO diets was 2.2 meq/kg and 120.6 meq/kg, respectively. The OFO diet resulted in a decrease in growth, muscularity, nutritional value of fatty acids in the muscle, and density of myofibers. In the OFO group, the mRNA expression levels of lipolysis genes including lipoprotein lipase (<em>lpl</em>) and hormone-sensitive lipase (<em>hsl</em>) in the muscle were upregulated compared with the FFO group, while, the mRNA expression level of acetyl CoA carboxylase was downregulated. Additionally, under the positive ion mode, the levels of DHA deposition in phosphatidylcholine and phosphatidyl ethanolamine were reduced in the muscle of the OFO group compared to the FFO group. A total of 68 proteins were identified in the muscle, of which 42 were up-regulated and 26 were down-regulated. The KEGG pathway enrichment analysis revealed that the major pathways of the differentially abundant proteins were the Wnt signaling pathway, TGF-beta signaling pathway, and proteoglycans in cancer. In summary, the OFO diet negatively affected growth, muscularity, and nutritional value. It also inhibited the transformation of myofiber types and led to the apoptosis of myofibers.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 1","pages":"Article 100321"},"PeriodicalIF":5.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143171582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial diversity and biogeography across gastrointestinal tracts of Takifugu pufferfish revealed by full-length 16S amplicon sequencing","authors":"Xingkun Jin ,&nbsp;Yan Shi ,&nbsp;Zhenlong Sun ,&nbsp;Yaohui Wang ,&nbsp;Zhe Zhao","doi":"10.1016/j.watbs.2024.100314","DOIUrl":"10.1016/j.watbs.2024.100314","url":null,"abstract":"<div><div>Fish, which are vital for both aquatic ecosystem functionality and global food supply, rely heavily on their gastrointestinal tract (GIT) microbiota for the digestion that underpins their growth and health. <em>Takifugu</em> pufferfish, which are an example of species evolved through adaptive radiation, possess a GIT that is specialized for antipredator defense and gluttonous feeding behaviors, offering a unique model to explore the effects of GIT compartmentalization and host genetics on gut microbial communities. Here we compiled 78 full and partial-length 16 ​S rRNA amplicon datasets across three anteroposteriorly distinct intestinal sites in a cohort of cohabitating artificial hybrid and purebred <em>Takifugu</em> pufferfishes. Our findings reveal a compositional and functional biogeography of pufferfish gut microbiota along the GIT and between host genetics. Additionally, the differential abundance of specific amplicon sequence variants and their correlation with host genetic backgrounds and intestinal sections highlight the role of environmental filtering in shaping microbial communities, with certain bacterial taxa exhibiting strong preferences for particular intestinal sites or genetic backgrounds, suggesting potential localized adaptation or functional specialization. This study enhances our understanding of the intricate interplay between host genetics, gut anatomy, and microbiota in fish, underscoring the importance of detailed microbial profiling in conservation efforts and aquaculture practices, and emphasizing the necessity of integrating full-length 16 ​S rRNA sequencing with partial-length datasets to comprehensively understand microbial diversity and function, paving the way for improved fish health management and sustainable aquaculture strategies.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"4 1","pages":"Article 100314"},"PeriodicalIF":5.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}