Water Biology and Security最新文献_第2页

Towards a low-carbon footprint: Current status and prospects for aquaculture 实现低碳足迹：水产养殖的现状和前景

IF 5.1

Water Biology and Security Pub Date : 2024-10-01 DOI: 10.1016/j.watbs.2024.100290

{"title":"Towards a low-carbon footprint: Current status and prospects for aquaculture","authors":"","doi":"10.1016/j.watbs.2024.100290","DOIUrl":"10.1016/j.watbs.2024.100290","url":null,"abstract":"<div><div>Aquaculture is one of the fastest-growing agricultural sectors globally and is becoming increasingly important for producing sustainable and healthy diets with relatively low climate impacts. In comparison to livestock production, especially beef, seafood production has lower carbon emissions. Additionally, some species can extract carbon from aquatic environments, contributing to an extended carbon cycle. However, the potential for negative environmental consequences in aquaculture production might increase with the growing global population and demand for aquatic food. Herein, we review the environmental consequences of aquaculture production and the potential effects of different aquatic products on greenhouse gas (GHG) emissions. We also summarize approaches to improving environmental footprints for mitigating global climate change. Key elements, including improving feed efficiency, selecting suitable farmed species, and implementing sustainable farming practices and management in aquaculture, are discussed, as well as the trade-offs between aquaculture and capture fisheries. Crucially, performance gaps among farmed species underscore the potential for consumer-oriented advice to significantly reduce the effects of aquaculture on climate change. However, uncertainties and knowledge gaps related to carbon sequestration and GHG emissions in aquaculture ponds necessitate further research. Overall, holistic evaluation and strategic intervention regarding GHG emissions are fundamental and essential for achieving a sustainable, low-carbon future for aquaculture.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141853877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipidome and proteome analyses provide insights into Mariana Trench Snailfish (Pseudoliparis swirei) adaptation to the hadal zone 脂质体和蛋白质组分析有助于了解马里亚纳海沟蜗牛鱼（Pseudoliparis swirei）对哈达区的适应情况

IF 5.1

Water Biology and Security Pub Date : 2024-10-01 DOI: 10.1016/j.watbs.2024.100295

{"title":"Lipidome and proteome analyses provide insights into Mariana Trench Snailfish (Pseudoliparis swirei) adaptation to the hadal zone","authors":"","doi":"10.1016/j.watbs.2024.100295","DOIUrl":"10.1016/j.watbs.2024.100295","url":null,"abstract":"<div><div>The hadal zone environment, characterized by extreme hydrostatic pressure, low temperatures, and limited food availability, presents substantial survival challenges for deep-sea fish species. In this study, we captured five deep-sea fish species (<em>Bathysaurus mollis</em>, <em>Coryphaenoides rudis</em>, <em>Ilyophis</em> sp., <em>I</em>. <em>brunneus</em> and <em>Pseudoliparis swirei</em>) from the Mariana Trench at depths ranging from 2027 to 7125 m, by employing China's “Exploration I and Exploration II”. By combining lipidomic and proteomic analyses, we aimed to explore the genetic basis of adaptive evolution to the hadal zone in fish. The results indicate several key findings: (1) <em>P</em>. <em>swirei</em> (Mariana hadal snailfish, MHS) may enhance energy storage and utilization during prolonged fasting periods by significantly increasing liver tissue levels of cholesterol ester (CE), ether-linked triacylglycerol (TG-O), coenzyme Q (CoQ), and ATPase content; (2) MHS could maintain membrane fluidity under high pressure by increasing the proportion of unsaturated fatty acids while reducing levels of cholesterol and phosphatidylethanolamine (PE) content. (3) The regulation of lipid types and ratios could increase the risk of lipid peroxidation. To counter oxidative stress, MHS likely elevates monounsaturated fatty acid content and enhances antioxidants such as transferrin and heat shock proteins. Overall, this study provides new insights into the adaptive mechanisms of MHS to deep-sea conditions through the lipidome and proteome analyses, thus broadening our understanding of its resilience in the hadal zone.</div></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142535852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental impact assessments should include rigorous scientific peer review 环境影响评估应包括严格的科学同行审查

IF 5.1

Water Biology and Security Pub Date : 2024-07-01 DOI: 10.1016/j.watbs.2024.100269

{"title":"Environmental impact assessments should include rigorous scientific peer review","authors":"","doi":"10.1016/j.watbs.2024.100269","DOIUrl":"10.1016/j.watbs.2024.100269","url":null,"abstract":"<div><p>Twenty USA states or jurisdictions and 125 nations have modeled national environmental policies after the National Environmental Policy Act. That act mandates that federal agencies initiate environmental impact statements (EISs) when substantive environmental or human health consequences are likely because of an agency action related to proposed development projects. The science used to inform the EIS process, however, does not require independent scientific peer review (ISPR) in the USA or most other nations. But ISPR is needed for governments to accurately inform the EIS decision-making and public reporting processes. Instead, science is routinely manipulated during EIS reviews to generate expedient project outcomes with substantially negative ecological, political, and long-term economic consequences. We provide four examples of EISs that lack ISPR, as well as four examples where reviews by independent scientists were helpful to improve agency decisions. We also recommend that independent scientists (no affiliation with the project proponents or agencies overseeing projects) be used to help assess potential environmental and socio-economic impacts, as well as offer appropriate risk assessments, study designs, and monitoring timeframes. We conclude that nations should convene formal reviews using independent scientists as a form of peer review in the EIS process.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000301/pdfft?md5=69bd67fbbddbbcfa1d30ca936e4c6ef5&pid=1-s2.0-S2772735124000301-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141731967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An improved method for tubulin staining of ciliated eukaryotes 纤毛真核生物管蛋白染色的改进方法

IF 5.1

Water Biology and Security Pub Date : 2024-07-01 DOI: 10.1016/j.watbs.2024.100274

引用次数: 0

A recombinase polymerase amplification with lateral flow assay for rapid on-the-spot detection of Aeromonas salmonicida 用于现场快速检测沙门氏菌的重组酶聚合酶扩增侧流试验

IF 5.1

Water Biology and Security Pub Date : 2024-07-01 DOI: 10.1016/j.watbs.2024.100272

{"title":"A recombinase polymerase amplification with lateral flow assay for rapid on-the-spot detection of Aeromonas salmonicida","authors":"","doi":"10.1016/j.watbs.2024.100272","DOIUrl":"10.1016/j.watbs.2024.100272","url":null,"abstract":"<div><p><em>Aeromonas salmonicida</em> is a common pathogen of salmonid fishes that poses a significant threat to the fresh water and marine culture industry, potentially resulting in huge economic losses. To prevent and control fish diseases caused by <em>A. salmonicida</em>, rapid and effective diagnostic approaches must be developed, and which are important for routine monitoring and clinical care. By combining recombinase polymerase amplification (RPA) technology with a visible lateral flow strip (RPA-LF), we have enhanced both the precision of RPA detection and the convenience of real-time monitoring. In this study, we introduce a robust method for detecting <em>A. salmonicida</em> using RPA-LF. This assay specifically targets the ASA_1441 gene of <em>A. salmonicida</em>, ensuring high specificity, without cross-reactivity with other prevalent fresh water or marine pathogens. The optimal amplification temperature of the RPA assay was 39 °C. Its sensitivity extends to as low as 100 fg of purified DNA, representing more than 1000-fold higher sensitivity than conventional PCR methods. Furthermore, to enhance the usability of the RPA-LF assay, we developed a rapid sample preparation method using cellulose dipsticks for nucleic acid extraction. This method achieves a limit of detection (LOD) as low as 1.67 CFU/μL and completes the entire process within 20 min. In conclusion, our findings present a rapid and precise tool for monitoring <em>A. salmonicida</em> infection in aquaculture and marine culture. This advancement offers valuable insights for effective disease prevention and control strategies.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000337/pdfft?md5=126901cd7c2312fc1afeba1f805d4806&pid=1-s2.0-S2772735124000337-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141139412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity in freshwater scuticociliates (Alveolata, Ciliophora, Scuticociliatia): Two new species isolated from a wetland in northern China, focusing on their morphology, taxonomy, and molecular phylogeny, with establishment of a new family Glauconematidae fam. nov. 淡水纤毛虫的多样性：从中国北部湿地分离出的两个新种，重点研究其形态学、分类学和分子系统学，并建立了一个新科Glauconematidae fam.

IF 5.1

Water Biology and Security Pub Date : 2024-07-01 DOI: 10.1016/j.watbs.2024.100275

{"title":"Diversity in freshwater scuticociliates (Alveolata, Ciliophora, Scuticociliatia): Two new species isolated from a wetland in northern China, focusing on their morphology, taxonomy, and molecular phylogeny, with establishment of a new family Glauconematidae fam. nov.","authors":"","doi":"10.1016/j.watbs.2024.100275","DOIUrl":"10.1016/j.watbs.2024.100275","url":null,"abstract":"<div><p>In the aquatic microbial food web, scuticociliates are diverse and predominant in almost all kinds of biotopes while extremely confused regarding their taxonomy and systematic relationships within the subclass Scuticociliatia. Here we provide detailed descriptions of two new and one known species, <em>Glauconema sinica</em> sp. nov., <em>Pleuronema parasetigerum</em> sp. nov., and <em>Histiobalantium natans viridis</em>, isolated from a freshwater wetland in northern China, along with their molecular phylogeny based on SSU rRNA gene sequences. Our results show: (1) both molecular and morphological data strongly support that the isolated lineage containing the genera <em>Glauconema</em>, <em>Miamiensis</em>, <em>Paramesanophrys</em>, and <em>Anophryoides</em> should be identified as a new taxon at the family level in the order Philasterida; hence, a new family, Glauconematidae fam. nov., is established. This new family is mainly characterized by the polymorphic life cycle of its members (macrostome and microstome stages); the trophont (or macrostome) has closely spaced membranelles 1–3, whereas in the tomite (or microstome) membranelle 1 is distant from membranelle 2. (2) Phylogenetic analyses using novel data for these species revealed that all three cluster with their congeners, supporting the validity of the genera to which they belong. Molecular information also supports the monophyly of the two main scuticociliate orders Pleuronematida and Philasterida.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000362/pdfft?md5=d440f26da58f3a6edfafb53c9107b4d2&pid=1-s2.0-S2772735124000362-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141391786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thirty years of change: Assessing the dynamics of fish communities in Daya Bay, a semi-enclosed coastal ecosystem of the South China sea 三十年的变化：评估南海半封闭海岸生态系统大亚湾的鱼类群落动态

IF 5.1

Water Biology and Security Pub Date : 2024-07-01 DOI: 10.1016/j.watbs.2024.100268

{"title":"Thirty years of change: Assessing the dynamics of fish communities in Daya Bay, a semi-enclosed coastal ecosystem of the South China sea","authors":"","doi":"10.1016/j.watbs.2024.100268","DOIUrl":"10.1016/j.watbs.2024.100268","url":null,"abstract":"<div><p>Daya Bay is a semi-enclosed bay in the South China Sea which is a coastal ecosystem with significant ecological and economic value that is subjected to a variety of anthropogenic disturbances. However, long-term variation in fish community structure in this Bay remains uncertain. In this study, we aimed to explore the changing characteristics of fish species composition and community structure in Daya Bay during the past 30 years using data from 1989 to 2021 from bottom trawl surveys and historical literature data. As of now, a total of 544 species of fish have been identified in the Bay, belonging to 24 orders and 125 families. The structure of the fish community in the bay has changed over time. Firstly, there has been a clear decrease in the number of species and biomass of large fish. Secondly, the number of fish constituent orders and families has decreased. Moreover, both the average taxonomic distinctness and variation in taxonomic distinctness decreased from 1989 to 2021. Thirdly, there is low similarity among fishes at different time phases and distinct changes in the composition of dominant species. Present results indicate that the fish community structure in Daya Bay has tended to reflect a decline in diversity, shifts in composition, and shrinking of fish body size in the past 30 years. This study improves our understanding of fish species composition and its drivers over time in Daya Bay, providing important knowledge to support the protection and restoration of this ecosystem and the important ecosystem services it provides.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000246/pdfft?md5=5aaea382f3483608d5f68bd1e7a47ae9&pid=1-s2.0-S2772735124000246-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140768914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Species composition and succession of coral reef fishes in Huaguang Reef, Xisha Islands 西沙群岛华光礁珊瑚礁鱼类的物种组成与演替

IF 5.1

Water Biology and Security Pub Date : 2024-07-01 DOI: 10.1016/j.watbs.2024.100273

{"title":"Species composition and succession of coral reef fishes in Huaguang Reef, Xisha Islands","authors":"","doi":"10.1016/j.watbs.2024.100273","DOIUrl":"10.1016/j.watbs.2024.100273","url":null,"abstract":"<div><p>A list of coral reef fish species of Huaguang Reef was compiled in 2023 using hand fishing, diving fishing, underwater video, and eDNA detection, combined with historical data from 1979 and archival records from the South China Sea Fisheries Research Institute of the Chinese Academy of Fisheries Sciences for the years of 1998, 1999, 2003, and 2005. Successional characteristics of coral reef fishes through time were also explored. The findings revealed: (1) A total of 299 coral reef fish species were identified in Huaguang Reef, categorized into 2 classes, 16 orders, and 56 families, with Perciformes exhibiting the highest species count (234), constituting 78.26% of all species. (2) In comparison to the period of 1979–2005, there was a reduction in the number of species at the order and family levels in 2023 at Huaguang Reef. Both the average taxonomic distinctness (Δ+) and the variation in taxonomic distinctness (Λ+) exhibited a declining trend, indicating disturbances in the fish ecosystem. (3) The proportion of herbivorous fish at Huaguang Reef remained relatively stable in 2023 compared to the 1979–2005 period. Notably, the similarity coefficient of herbivorous fish was the highest among different feeding types, signifying a degraded state of the Huaguang Reef habitat. (4) In contrast to the 1979–2005 period, a significant decline was observed in large-sized and carnivorous fish species at Huaguang Reef in 2023. The low similarity coefficients for both categories, with undiscovered fish species accounting for 58.49% and 45.76% of their respective taxa in the total list, respectively, suggested a notable impact of overfishing on coral reef fishes. The succession patterns revealed in this study provide a theoretical foundation for advancing the sustainable development of coral reef fish resources in the Xisha Islands and offer valuable insights for the protection and management of coral reef fishes.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000349/pdfft?md5=9dcef2d93de6d800ed80024bae1600f3&pid=1-s2.0-S2772735124000349-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141731969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal variation of microeukaryotic biodiversity and biotic condition associated with disinfectants after the COVID-19 lockdown in Wuhan, China 中国武汉 COVID-19 封锁后与消毒剂相关的微真核生物多样性和生物条件的时空变化

IF 5.1

Water Biology and Security Pub Date : 2024-07-01 DOI: 10.1016/j.watbs.2024.100266

{"title":"Spatiotemporal variation of microeukaryotic biodiversity and biotic condition associated with disinfectants after the COVID-19 lockdown in Wuhan, China","authors":"","doi":"10.1016/j.watbs.2024.100266","DOIUrl":"10.1016/j.watbs.2024.100266","url":null,"abstract":"<div><p>Intensive application of chlorine-based disinfectants driven by the COVID-19 pandemic was suspected to be detrimental to receiving water ecosystems, but with little field evidence. We characterized the occurrences of typical disinfectants and microeukaryotic communities in surface waters associated with three wastewater treatment plants (WWTP)-river systems one year after the lockdown in Wuhan, China. Trihalomethanes (THMs) declined from summer 2020 (0.01–1.82 μg/L) to summer 2021 (0.01–0.95 μg/L), whereas quaternary ammonium salts (QAs) increased from summer 2020 (0.53–6.35 ng/L) to summer 2021 (8.49–191 ng/L). Biodiversity monitoring with environmental DNA (eDNA) revealed significant temporal variation in microeukaryotic community composition. The monitored disinfectants were correlated with some eukaryotic communities as demonstrated by redundancy analysis. For example, QAs were positively related to Cryptophyta relative abundance, but negatively related to Rotifera relative abundance. A microeukaryote-based multimetric index indicated ecological impairment near the Han River WWTP outlet in 2020. Our findings indicate the influence of heavily used disinfectants on river microeukaryotic communities, and the usefulness of assessing mid-term ecological risks from disinfectants in the post COVID-19 era.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000222/pdfft?md5=63bdc043a6d4cc836bb79b061bc7cd04&pid=1-s2.0-S2772735124000222-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140796949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chinese wisdom and modern innovation of aquaculture 中国智慧与现代水产养殖创新

IF 5.1

Water Biology and Security Pub Date : 2024-07-01 DOI: 10.1016/j.watbs.2024.100271

{"title":"Chinese wisdom and modern innovation of aquaculture","authors":"","doi":"10.1016/j.watbs.2024.100271","DOIUrl":"10.1016/j.watbs.2024.100271","url":null,"abstract":"<div><p>Aquaculture originates from ancient Chinese wisdom, and is considered the most efficient food-producing industry. About 70 years ago, New China proposed an industrial approach to fish production and made a policy decision to begin modern aquaculture development. As one of lucky persons since the early 1980s, I have committed my scholarly career to the studies on genetics and breeding biotechnology of polyploid gibel carp (<em>Carassius gibelio</em>). Here, several significant recent innovations, such as the proposition of the amphitriploid concept, the association of ploidy changes with reproduction transition, the finding of a unique ameio-fusiongenesis mode, the establishment of a controllable fertility approach, the creation of intermuscular bone-free mutants, as well as the recreation of desirable goldfish, are introduced and emphasized from an active participant of these innovations. Additionally, some modern innovations and contributions are also highlighted in other aspects of aquaculture including feed development, disease control, facilities and equipment upgrading, as well as paradigm and mode changes. This comprehensive synthesis of ancient Chinese wisdom and modern innovations seeks to provide valuable insights into the field from a historical basis.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":null,"pages":null},"PeriodicalIF":5.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000325/pdfft?md5=0ea102eb650fc1b27c077fb532e7be26&pid=1-s2.0-S2772735124000325-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141030189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0