MicroRNA分析揭示了感染LMBV的大口黑鲈幼鱼（Micropterus salmoides）免疫反应和代谢变化

IF 5.1 Q1 ENVIRONMENTAL SCIENCES

Water Biology and Security Pub Date : 2025-04-01 DOI:10.1016/j.watbs.2024.100327

Xiaodan Liu , Liwen Zhang , Xu Tan , Mengya Guo , Weiguang Kong , Zhenhua An

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引用次数: 0

摘要

大口黑鲈病毒（LMBV）在大口黑鲈成鱼和幼鱼中都表现出高致病性，给水产养殖业造成了巨大的经济损失。MicroRNAs （miRNAs）在控制病毒感染和宿主免疫反应中起着至关重要的作用，在疾病的治疗和诊断中具有重要的价值。然而，目前对大口黑鲈LMBV感染相关miRNA表达谱的研究还不够。本研究试图通过miRNA测序研究miRNA在大口黑鲈LMBV感染引发的潜在免疫反应和代谢改变中的作用和分子机制。经过质量筛选，感染组和对照组总共产生了1.4273亿个干净reads，长度主要在22 nt。鉴定了1718个已知的mirna，其中包括238个差异表达mirna （DEMs）。此外，还预测了400个新的mirna，其中36个是dem。为了进一步了解dem的免疫和代谢相关生物学功能，我们进行了靶基因预测。KEGG通路分析显示，LMBV影响了内吞作用、嘌呤代谢、磷脂酰肌醇、脂肪酸生物合成和吞噬体信号系统等通路，突出了mirna在免疫应答和代谢改变中的重要作用。此外，miRNA-mRNA相互作用网络揭示了以代谢和免疫相关途径为基础，参与赋予病毒感染抗性的关键mirna及其相应的靶基因。随机选择10个dem进行实时定量PCR (qRT-PCR)，结果显示与测序数据一致的表达模式。这些发现验证了mirna对大口黑鲈LMBV的免疫和代谢调控功能，为虹膜病毒相关疾病的预防和管理提供了有价值的视角。

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MicroRNA profiling yields immune response and metabolic changes in juvenile largemouth bass (Micropterus salmoides) infected with LMBV

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MicroRNA profiling yields immune response and metabolic changes in juvenile largemouth bass (Micropterus salmoides) infected with LMBV

The largemouth bass virus (LMBV) exhibits high pathogenicity in both adult and juvenile largemouth bass, causing substantial economic losses within the aquaculture industry. MicroRNAs (miRNAs) are crucial in controlling viral infections and the host's immune responses, making them significantly valuable in the treatment and diagnosis of diseases. Nevertheless, research on miRNA expression profiles associated with LMBV infection in largemouth bass is currently insufficient. This research attempts to investigate the roles and molecular mechanisms of miRNAs in the potential immune response and metabolic alterations triggered by LMBV infection in largemouth bass using miRNA sequencing. Following quality screening, the infection group and control group yielded a combined total of 142.73 million clean reads, with lengths predominantly at 22 nt. 1718 known miRNAs were identified, including 238 differentially expressed miRNAs (DEMs). In addition, 400 novel miRNAs were predicted, 36 of which were DEMs. To gain further insight into the immune and metabolic related biological functions of DEMs, target gene prediction was conducted. KEGG pathway analysis revealed that LMBV impacted pathways such as Endocytosis, Purine metabolism, Phosphatidylinositol, Fatty acid Biosynthesis, and Phagosome signaling systems, highlighting the vital role of miRNAs in immune responses and metabolic alterations. Furthermore, the miRNA-mRNA interaction network revealed crucial miRNAs and their corresponding target genes involved in conferring resistance against viral infections by utilizing metabolic and immune related pathways as the foundation. Ten DEMs were selected at random for real-time quantitative PCR (qRT-PCR), and results exhibited expression patterns that were consistent with sequencing data. These findings validate the immune and metabolic regulatory function of miRNAs against LMBV in largemouth bass, offering valuable perspectives for the prevention and management of illnesses linked to iridoviruses.

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Water Biology and Security

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4.10

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0.00%

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