Water Biology and Security最新文献

{"title":"Bloom-forming cyanobacteria and dinoflagellates in five Argentinian reservoirs: Multi-year sampling","authors":"Florencia Soledad Alvarez Dalinger ,&nbsp;Borja Claudia Nidia ,&nbsp;Verónica Laura Lozano ,&nbsp;Liliana Beatriz Moraña ,&nbsp;María Mónica Salusso","doi":"10.1016/j.watbs.2023.100232","DOIUrl":"https://doi.org/10.1016/j.watbs.2023.100232","url":null,"abstract":"<div><p>Harmful algal blooms are important threats to reservoir condition. Over a 15-year period, we sampled five Argentinian reservoirs to identify the responsible species for harmful algal blooms and determine the water quality factors driving their occurrence. These reservoirs exhibit diverse morphological and hydrological characteristics and are distributed across varying climatic zones. Cyanobacterial blooms included an array of species, including toxin producers such as <em>Raphidiopsis raciborskii, Aphanizomenon gracile</em>, and <em>Microcystis aeruginosa</em>. In contrast, dinoflagellate blooms were predominantly characterized by <em>Ceratium</em>. The cyanobacterial blooms primarily occurred in the shallower reservoirs located in warmer regions, whereas dinoflagellate blooms occurred where temperatures were lower. The most intense blooms occurred during the summer, and although cyanobacterial and chlorophyte blooms co-occurred, they never coexisted with dinoflagellate blooms. We identified cyanotoxins in the reservoirs over the past three years, a phenomenon previously unreported in the region. Given that these reservoirs are drinking water sources for human populations, ongoing and systematic monitoring is needed to protect public health. Controlling the proliferation of algae and cyanobacteria in reservoirs requires resource management at the watershed level, and in the case of the Itiyuro and El Limón, requires binational management with Bolivia.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 1","pages":"Article 100232"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735123001270/pdfft?md5=0407745454e31815798aa73ba5d8733c&pid=1-s2.0-S2772735123001270-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139419385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cladophora as ecological engineer: A new test from the largest lake of Qinghai-Tibet plateau with filamentous algal blooms","authors":"Zhihua Wu ,&nbsp;Xigong Yuan ,&nbsp;Xiong Xiong ,&nbsp;Hongyi Ao ,&nbsp;Chenxi Wu ,&nbsp;Guoxiang Liu ,&nbsp;Huan Zhu","doi":"10.1016/j.watbs.2023.100210","DOIUrl":"10.1016/j.watbs.2023.100210","url":null,"abstract":"<div><p>Filamentous algae blooms (FABs) have been increasing globally in recent years, and their presence can have both harmful and beneficial effects on aquatic ecosystems. As one of the most common FABs, <em>Cladophora</em> blooms have been reported in the lakes of the Qinghai-Tibet Plateau during the past few years. However, there have been few studies focused on how FABs impact other aquatic organisms, especially in alpine lakes since these are at the forefront of responding to global climate change. In this study, the phytoplankton communities in different regions of Qinghai Lake were profiled in different seasons using meta-barcode sequencing. The phytoplankton assemblages in areas with <em>Cladophora</em> blooms were compared to those without <em>Cladophora</em>. The phytoplankton community structure correlated with physicochemical properties including water temperature, electrical conductivity, nitrate, and the presence or absence of <em>Cladophora</em> blooms. The relative abundance of Bacillariophytes was found to be higher in zones with <em>Cladophora</em> blooms than in other regions. Significant seasonal changes in phytoplankton biomass and β diversity were observed in zones with <em>Cladophora</em> blooms. Growth and microbial degradation of <em>Cladophora</em> can change the pH, dissolved oxygen, secchi depth, and nitrate. Together with seasonal temperature and electrical conductivity changes, <em>Cladophora</em> growth can significantly impact the phytoplankton biomass, community dissimilarity and assembly process. These results showed that <em>Cladophora</em> plays a key role in littoral aquatic ecosystem ecology.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 1","pages":"Article 100210"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735123000963/pdfft?md5=e2bb7a6a0998bbfc031c23cf53d5abbf&pid=1-s2.0-S2772735123000963-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77099141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plastic effects on marine and freshwater environments","authors":"Fabiula Danielli Bastos de Sousa","doi":"10.1016/j.watbs.2023.100228","DOIUrl":"10.1016/j.watbs.2023.100228","url":null,"abstract":"<div><p>In the 1930s, plastic items started to acquire popularity. Since then, they have overcome many barriers and are now important to many facets of society. Post-consumer plastic, however, is an environmental problem of increasing concern. Marine and freshwater environments are being impaired by plastic pollution, which is mostly the result of improper post-use plastic disposal. Herein I review recent literature on plastic effects to marine and freshwater environments, based on a Scopus search. Authors' keywords were analyzed and mapped. Results depicted microplastics as a global concern for the environment, animals, and human health, and was identified as a primary research focus and emerging topic concerning plastic effects on marine and freshwater environments. The foremost research gap is the lack of clear connections between the effects of plastic on human health, animal health, and the environment. Water pollution and its consequences on marine and freshwater environments are attributed primarily to human activity.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 1","pages":"Article 100228"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735123001233/pdfft?md5=7e7f3d9fc573f1b6d3f210dcd89c4952&pid=1-s2.0-S2772735123001233-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136127703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diet variability of snappers (Teleostei: Lutjanidae) in a bay-to-reef ecosystem of the Mexican Caribbean","authors":"León Felipe Martínez-Juárez ,&nbsp;Juan J. Schmitter-Soto ,&nbsp;Nancy Cabanillas-Terán ,&nbsp;Norman Mercado-Silva","doi":"10.1016/j.watbs.2023.100211","DOIUrl":"10.1016/j.watbs.2023.100211","url":null,"abstract":"<div><p>Fishes use environmental resources differently. Snapper ontogenic movements among coastal habitats lead to diverse diets. We investigated the diets of seven snapper species (<em>Lutjanus apodus</em>, <em>L. analis</em>, <em>L. griseus</em>, <em>L. jocu</em>, <em>L. mahogoni</em>, <em>L. synagris</em>, and <em>Ocyurus chrysurus</em>) in three interconnected coastal environments in the western Caribbean: Río Huach channel, Chetumal/Corozal bay, and the adjacent Xcalak reef lagoon. These three encompass all habitats used by the seven species in the study area. Snapper diets were mostly composed of fish and crustaceans, but dominance of either group varied among the studied habitats; for example, fish were the main prey for <em>L. apodus</em> from the reef lagoon zone, but second in the bay. Snappers are piscivorous-invertivorous mesopredators; the species that relied the most on fishes were <em>L. griseus</em> and <em>L. jocu</em>, whereas <em>O</em>. <em>chrysurus</em> preferred crustaceans. Stomach content results revealed a high degree of dietary overlap among species. Our findings suggested a tradeoff in relative weight between fish and crustaceans as the main food source of snappers at these connected areas, supporting the hypothesis of niche segregation. Our results can be used as a baseline to further our understanding of the ecology and differences in resource use by snapper species in an area undergoing important anthropogenic changes.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 1","pages":"Article 100211"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735123000975/pdfft?md5=a4cb4a5dd2ad1426f87dba374ae976ae&pid=1-s2.0-S2772735123000975-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76360900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessments and diagnoses of aquatic ecosystem integrity based on integrity requirements of ecosystem service targets","authors":"Hong-Zhu Wang","doi":"10.1016/j.watbs.2023.100230","DOIUrl":"10.1016/j.watbs.2023.100230","url":null,"abstract":"<div><p>This paper critically reviews the progress in ecosystem integrity (health) assessment of inland waters and provides future directions for assessment. Current biotic integrity assessments mainly use multimetric indices and predictive models to evaluate overall health status; the criteria largely rely on pristine reference sites, but such an approach is not applicable to unique natural waters and irreversibly modified waters. Biotic diagnostic assessments are still in the exploratory stage and can only diagnose possible stressor types and wide-ranges of their intensities through empirical models linking stressors and species-trait-indices. Current chemical integrity assessments mainly use criteria determined by small-scale ecotoxicity tests, rather than quantitative relationships developed between chemical factors and biotic effects in real ecosystems, thus potentially under or over-estimating pollutant toxicity. Current physical integrity assessments focus on overall habitat quality, rather than quantitative habitat requirements, and thus cannot provide quantitative support for ecological restoration and conservation. Current hydrological integrity (environment flow) assessments largely depend on quantitative relationships between hydrological regimes with a few species and single groups, rather than with whole communities, and fail to comprehensively diagnose hydrological causes of biotic resource decline. In the future, integrity assessments need to be based on ecosystem integrity requirements of ecosystem service targets: first, there is a need to build quantitative relationships between biotic integrity and ecosystem services, assess requirements of community structure and function for service goals, and establish biotic integrity assessment methods; second, we must build quantitative relationships between biotic integrity and abiotic integrity in real ecosystems, assess chemical, physical, and hydrological integrity requirements of biotic needs, and establish diagnostic assessment methods.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 1","pages":"Article 100230"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735123001257/pdfft?md5=2d36d61e0478db28b52efb454f9c682a&pid=1-s2.0-S2772735123001257-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136011001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxygen potentiates antiviral ability of zebrafish in response to SVCV infection","authors":"Zixuan Wang ,&nbsp;Chunchun Zhu ,&nbsp;Xiaoyun Chen ,&nbsp;Hongyan Deng ,&nbsp;Xueyi Sun ,&nbsp;Qian Liao ,&nbsp;Shuke Jia ,&nbsp;Wen Liu ,&nbsp;Yao Bai ,&nbsp;Wuhan Xiao ,&nbsp;Xing Liu","doi":"10.1016/j.watbs.2023.100207","DOIUrl":"https://doi.org/10.1016/j.watbs.2023.100207","url":null,"abstract":"<div><p>Oxygen (O₂) profoundly influences the physiological processes of aerobic organisms through a range of mechanisms. Recently, increasing evidence has revealed the relationship between viral infection and oxygen levels. However, due to a lack of feasible methods and <em>in vivo</em> models, how oxygen directly affects antiviral capability remains largely unknown. In contrast to terrestrial animals, fish live in water for life, where oxygen levels change more frequently than on land in areas with similar altitude. Therefore, fish appear to be ideal organisms for elucidating the effect of oxygen levels on antiviral responses. In this study, we report that zebrafish under low oxygen conditions are more susceptible to SVCV infection. Further assays indicate that low oxygen tension not only suppresses SVCV-induced IFN activation but also promotes SVCV replication in both zebrafish cell lines and zebrafish. This study provides novel insights into the effect of oxygen on antiviral responses and virus replication.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 4","pages":"Article 100207"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49728836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced pigmentation and thyroid hormone disruption in zebrafish embryos caused by industrial sludge near Bohai Bay, China","authors":"Wenjing Dong ,&nbsp;Xiaoyu Yin ,&nbsp;Chelimuge Qi ,&nbsp;Tingting Wei ,&nbsp;Lijia Wei ,&nbsp;Jingfeng Yang ,&nbsp;Jingli Mu ,&nbsp;Hiroki Teraoka ,&nbsp;Wu Dong","doi":"10.1016/j.watbs.2023.100190","DOIUrl":"https://doi.org/10.1016/j.watbs.2023.100190","url":null,"abstract":"<div><p>In recent years, pollution caused by the discharge of industrial wastewater into Bohai Bay has posed a potential threat to the health of surrounding residents. Sludge was collected from the outlet of a factory that discharges effluent into Bohai Bay, and alcohol extracts of sludge (SE) were prepared. We confirmed by UPLC-MS/MS analysis that the SE contained PAHs, including fluorene, pyrene, and phenanthrene. Zebrafish embryos as animal models were exposed to 0.1, 0.3, 0.5, 1 and 5 ​mg/mL SE from 2 to 4 ​h post-fertilization (hpf) until 120 hpf. The results showed that SE caused a concentration-dependent increase in mortality and a decrease in hatchability. We found that SE significantly reduced eye pigmentation and decreased the movement of embryos and larvae. In addition, SE decreased triiodothyronine (T3) content and down-regulated the mRNA expression of some thyroid hormone-related genes including <em>TPO</em> and <em>Thrβ</em>, and caused the up-regulation of <em>Dio2</em> and <em>Dio3</em> at 120 hpf. Exposure to three individual PAHs found in SE, namely fluorene, pyrene, and phenanthrene, caused morphological and transcriptional changes that were similar to those caused by SE exposure. These findings indicate that PAHs in SE can reduce the pigmentation of zebrafish, which may be related to the genetic changes associated with thyroid hormones, and that zebrafish eye pigmentation can be used as an indicator of PAHs exposure.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 4","pages":"Article 100190"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49733872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global concerns related to water biology and security: The need for language and policies that safeguard living resources versus those that dilute scientific knowledge","authors":"Robert M. Hughes ,&nbsp;James R. Karr ,&nbsp;Robert L. Vadas ,&nbsp;Dominick A. DellaSala ,&nbsp;Marcos Callisto ,&nbsp;Maria João Feio ,&nbsp;Teresa Ferreira ,&nbsp;Neels Kleynhans ,&nbsp;Renata Ruaro ,&nbsp;Chris O. Yoder ,&nbsp;J. Hal Michael","doi":"10.1016/j.watbs.2023.100191","DOIUrl":"https://doi.org/10.1016/j.watbs.2023.100191","url":null,"abstract":"<div><p>Increasingly, scientists and non-scientists, especially employees of government agencies, tend to use weak or equivocal language when making statements related to science policy and governmental regulation. We use recent publications to provide examples of vague language versus examples of strong language when authors write about regulating anthropogenic pressures on natural resources. Lifeless language is common in agency reports, policy documents, and even scientific papers published by academics. Such language limits success in regulating anthropogenic pressures on natural resources. This challenge must be recognized and countered as a driver of the condition of water and associated resources. We also list sources of vague wording, provide global examples of how ambiguous language and political influences have contributed to water resource degradation, discuss the recent history of science censorship, and offer possible solutions for more direct scientific discourse. We found that: (1) equivocal language was especially common in concluding statements and not only by government employees; (2) authors discussed confusing language concerns in an agency publication; and (3) agency employees sometimes used active, strong language. Key drivers of weak language include: (1) holding on to old paradigms and resisting new knowledge; (2) scientific uncertainty; (3) institutional manuscript review policies; (4) employment and funding insecurity; and (5) avoiding the appearance of advocacy. Examples associated with euphemistic language included climate change, flow and physical habitat alteration, dams, agriculture, mining, forestry, and fisheries, as well as resistance towards monitoring, assessing, and reporting ecological conditions. Suggestions for mitigating equivocal language involve employment protections and greater focus on scientific ethics. We conclude that natural resource scientists should resist calls to employ imprecise language. Instead, they should be strong advocates for prescriptive and protective natural resource actions—based on their science—to halt and reverse the systemic degradation of those resources.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 4","pages":"Article 100191"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49713226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nuclear events during conjugation in the poorly studied model ciliate Paramecium jenningsi","authors":"Xue Zhang ,&nbsp;Ruitao Gong ,&nbsp;Yaohan Jiang ,&nbsp;Xiaoteng Lu ,&nbsp;Chenyin Wu ,&nbsp;Lihan Wang ,&nbsp;Hongwei Ma ,&nbsp;Zhengxiang Zhang ,&nbsp;Weibo Song ,&nbsp;Khaled A.S. Al-Rasheid ,&nbsp;Adriana Vallesi ,&nbsp;Feng Gao","doi":"10.1016/j.watbs.2023.100201","DOIUrl":"https://doi.org/10.1016/j.watbs.2023.100201","url":null,"abstract":"<div><p>Ciliated protists are highly differentiated unicellular eukaryotes that possess special sexual processes (conjugation and autogamy) that rely on their unique nuclear dimorphism, i.e., the presence of both a germline micronucleus (MIC) and a somatic macronucleus (MAC) in a single cell. The sexual processes show a high diversity in different ciliates. To better understand the differentiation and evolution of sexual processes in closely related species, we investigated the nuclear events during conjugation in <em>Paramecium jenningsi</em> strain GZ, a poorly studied close relative of the well-known <em>P. aurelia</em> complex. The main results include: (1) the conjugation process takes about 48 ​h, including three prezygotic divisions (meiosis I, II, and mitosis) and three postzygotic divisions; (2) the MICs are dominated by the “parachute” phase at the prophase of meiosis I; (3) after meiosis II, a variable number of nuclei undergo the third prezygotic division, and the two products near the paroral cone become the genetically identical migratory and stationary pronuclei; (4) the synkaryon divides three times to form the MIC and the MAC anlagen, but only one nuclear product of the first postzygotic division completes the next two divisions; and (5) an extra cell division is required to complete the last step of conjugation, during which two MIC anlagen undergo mitotic division while two MAC anlagen are distributed between the daughter cells without division. The comparison of the nuclear events during conjugation in representatives of the class Oligohymenophorea reveals that usually there are three prezygotic divisions and a variable number of postzygotic divisions. However, the number of selected and differentiated nuclei after each division differs among species. This study provided a new model ciliate for further investigations of nuclear selection and differentiation as well as nuclear morphology during meiosis.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 4","pages":"Article 100201"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49734401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How to use live sampling tissues and archived specimens in cetacean stable isotope research","authors":"Tao Jin ,&nbsp;Ruilong Wang ,&nbsp;Renyong Wang ,&nbsp;Jiayi Xie ,&nbsp;Jinsong Zheng ,&nbsp;Fei Fan ,&nbsp;Kexiong Wang ,&nbsp;Ding Wang ,&nbsp;Jun Xu ,&nbsp;Zhigang Mei","doi":"10.1016/j.watbs.2023.100206","DOIUrl":"https://doi.org/10.1016/j.watbs.2023.100206","url":null,"abstract":"<div><p>Cetaceans are unique ecological engineers, and their restoration may have a crucial impact on the future structure of aquatic ecosystems, which calls for more investigations into their trophic ecology. Among current techniques, stable isotope analysis (SIA) has the advantages of non-invasive sampling and long timescales. However, the full benefits of SIA in cetacean research may not be achieved due to issues like different types of tissue between sampling methods and use of chemical preservation solutions in historical specimens. To address these challenges, we conducted a study on Narrow-ridged Finless Porpoises (<em>Neophocaena asiaeorientalis</em>). Multiple tissues from freshwater and marine subspecies, as well as tissues preserved using different solutions such as ethanol and formalin were collected for SIA. Linear mixed effects models were used for data analysis. Our results showed that, except for blubber, kidney, and stomach, differences between other tissues were correctable. In tissues from live sampling, we found no significant difference between blood and muscle, and skin could also be used for isotope analysis after proper correction. Ethanol preservation caused significant positive changes in δ¹³C and δ¹⁵N values of muscle, while formalin preservation caused negative changes in δ¹³C and δ¹⁵N. Our findings provide valuable insight into unifying data from stranded carcasses and live sampling, as well as correcting for the effect of chemical preservation on museum specimens. Findings from this research support further application of stable isotope analysis in the conservation of endangered finless porpoises, offer a reference for other similar cetaceans, and also provide guidance for chemical preservation when freezing conditions are not available.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"2 4","pages":"Article 100206"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49713317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}