Serodiagnosis and Immunotherapy in Infectious Disease最新文献_第3页

Toxoplasmosis and cross-reactivity: an analysis of the serological response by immunoblotting 弓形虫病和交叉反应:免疫印迹分析血清学反应

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1996-05-01 DOI: 10.1016/S0888-0786(96)80009-6

J. Dave, D.S. Tompkins, R.P. Eglin, J.P. Harford

引用次数: 1

Restriction of serological cross-reactivity between blood stages of Plasmodium falciparum and P. vivax following single malaria infections 单次疟疾感染后恶性疟原虫和间日疟原虫血分期血清学交叉反应性的限制

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1996-05-01 DOI: 10.1016/S0888-0786(96)80007-2

L.H. Carvalho , C.J.F. Fontes , A.A.M. Fernandes , A.U. Krettli

{"title":"Restriction of serological cross-reactivity between blood stages of Plasmodium falciparum and P. vivax following single malaria infections","authors":"L.H. Carvalho , C.J.F. Fontes , A.A.M. Fernandes , A.U. Krettli","doi":"10.1016/S0888-0786(96)80007-2","DOIUrl":"10.1016/S0888-0786(96)80007-2","url":null,"abstract":"<div><p>Serology is valuable for assessing malarial transmission levels, monitoring the effectiveness of control measures, detecting latent foci and screening blood donors in malaria-free areas. Only a <em>Plasmodium falciparum</em> antigen, obtained from culture, is commercially available for serological testing, the rational being the cross-reactivity among blood stages of different <em>Plasmodium</em> species. We have compared the frequency of cross-reactivity of sera from individuals who had recovered from clinical malaria caused by <em>P. vivax</em> (group Pv) or <em>P. falciparum</em> (group Pf) with blood stage antigens of either parasite. In both groups, a higher seropositivity, detected using indirect fluorescent antibody test (IFAT), was observed with the homologous antigen. Since the cross-reactivity observed with the heterologous antigen could reflect previous malaria infections, we analysed the reactions with sera from mono-infected individuals with either Pf or Pv and observed a low level of cross-reaction (∼ 20%). Our results suggest that the Pf antigen used in the routine of serology does not replace the Pv antigen, and that the two antigens should be used simultaneously to avoid false negative reactions, especially in primary infections.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"8 2","pages":"Pages 99-103"},"PeriodicalIF":0.0,"publicationDate":"1996-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0888-0786(96)80007-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77798398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Detection of anti-phenolic glycolipid I antibodies by ELISA and gelatin particle agglutination test in leprosy in São Paulo (SP), Brazil ELISA和明胶颗粒凝集试验检测巴西<s:1>圣保罗(SP)麻风病患者抗酚类糖脂I抗体

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1996-05-01 DOI: 10.1016/S0888-0786(96)80005-9

C.S. Melo , S.M.O.O. Nitrini , L.F.G. Siqueira

引用次数: 0

Comparison of four newly developed immunoblot assays with RIBA II for detection of HCV antibodies 四种新开发的免疫印迹法与RIBA II检测HCV抗体的比较

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1996-05-01 DOI: 10.1016/S0888-0786(96)80003-5

Hanns Hofmann

{"title":"Comparison of four newly developed immunoblot assays with RIBA II for detection of HCV antibodies","authors":"Hanns Hofmann","doi":"10.1016/S0888-0786(96)80003-5","DOIUrl":"10.1016/S0888-0786(96)80003-5","url":null,"abstract":"<div><p>ELISA, the usual screening test for HCV infection, may yield nonspecific results. Most laboratories, therefore, for corroboration perform PCR. However, a negative HCV-PCR does not prove nonspecifity of the initial ELISA test and therefore an immunoblot has to be performed. RIBA-II was used for that purpose for several years, but suffers from a high number of indeterminate results. We therefore compared RIBA II results of 75 sera with those of RIBA III, Matrix, Western Blot (Murex) and INNO-LIA tests. Of 34 sera that were positive in RIBA II, all were also positive in the four other immunoblots. Similarly, these 4 tests showed concordantly positive results in 13 of 27 RIBA II indeterminate sera. In the remaining 14 (RIBA II indeterminate) sera the four immunoblots displayed no uniform results but various combinations of positive, indeterminate and even negative results. Similar results were found with 13 RIBA II negative (ELISA positive) sera. These data may indicate less sensitivity as well as some nonspecificity of some of the immunoblots. In general, however, the four newly developed immunoblots proved to be more sensitive than RIBA II. This obviously is not caused by the inclusion of the NS<sub>5</sub>-antigen but by improvement of the conventional antigens. Only one serum was found in which the NS<sub>5</sub>-band was crucial for its positivity. In conclusion, for corroboration of some HCV-ELISA positive, PCR negative sera, more than one immunoblot may be necessary.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"8 2","pages":"Pages 79-83"},"PeriodicalIF":0.0,"publicationDate":"1996-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0888-0786(96)80003-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76622272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Immunoblotting reactivity of sera from patients with autoimmune connective tissue diseases against Epstein-Barr nuclear antigen (EBNA) polypeptides 自身免疫性结缔组织疾病患者血清对EBNA多肽的免疫印迹反应性

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1996-05-01 DOI: 10.1016/S0888-0786(96)80008-4

J. Ngou, M. Segondy

引用次数: 2

A study of IgM antibodies in diagnosis of acute infection by Toxoplasma gondii in Spain IgM抗体在西班牙刚地弓形虫急性感染诊断中的应用研究

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1996-05-01 DOI: 10.1016/S0888-0786(96)80004-7

J. Gutiérrez, M. Rodriguez, C. Maroto

引用次数: 3

Comparative evaluation of Abbott and Murex antibody assays and Roche Amplicor PCR for diagnosis of human hepatitis C 雅培和Murex抗体检测与罗氏扩增PCR诊断丙型肝炎的比较评价

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1996-05-01 DOI: 10.1016/S0888-0786(96)80011-4

D. Liu, J. Steele, S. Lloyd Jones, D. Leslie, J. Pedersen, R. Baird

{"title":"Comparative evaluation of Abbott and Murex antibody assays and Roche Amplicor PCR for diagnosis of human hepatitis C","authors":"D. Liu, J. Steele, S. Lloyd Jones, D. Leslie, J. Pedersen, R. Baird","doi":"10.1016/S0888-0786(96)80011-4","DOIUrl":"10.1016/S0888-0786(96)80011-4","url":null,"abstract":"<div><p>Of 135 serum samples from 135 patients suspected of hepatitis C virus (HCV) infection, 67 were detected by Abbott IMX antibody assay, 89 by Murex anti-HCV (version III), and 47 by Roche Amplicor polymerase chain reaction (PCR). Furthermore, 44 of the 62 positive serum samples by both Abbott and Murex antibody assays, 2 of the 27 positive samples by Murex antibody assay only, none of the 5 positive samples by Abbott antibody assay only, and one of the 43 negative samples by both Abbott and Murex antibody assays had measurable HCV RNA by Roche Amplicor PCR, suggesting active hepatitis C viremia. Whereas Abbott and Murex antibody assays were in agreement with each other in 103 of the 135 serum samples tested, they showed discrepancy with regard to the other 32. Despite generating a small percentage of false positives, Abbott and Murez antibody assays are useful in monitoring serum antibody levels of the past or continuing hepatitis C virus infection. Abbott IMX appears to be more specific than Murex anti-HCV (version III). The use of Roche Amplicor PCR provides a means of revealing active hepatitis C viremia, and helping clarify antibody indeterminate serum samples.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"8 2","pages":"Pages 121-124"},"PeriodicalIF":0.0,"publicationDate":"1996-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0888-0786(96)80011-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86187249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changing patterns of detection of viruses in respiratory specimens since introduction of monoclonal antibody-based immunofluorescence test for respiratory syncytial virus antigen 引入呼吸道合胞病毒抗原单克隆抗体免疫荧光检测方法以来呼吸道标本病毒检测模式的变化

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1996-05-01 DOI: 10.1016/S0888-0786(96)80006-0

A.M. Ashshi , D.J. Morris , A.D. Semple

{"title":"Changing patterns of detection of viruses in respiratory specimens since introduction of monoclonal antibody-based immunofluorescence test for respiratory syncytial virus antigen","authors":"A.M. Ashshi , D.J. Morris , A.D. Semple","doi":"10.1016/S0888-0786(96)80006-0","DOIUrl":"10.1016/S0888-0786(96)80006-0","url":null,"abstract":"<div><p>In our earlier studies, a rapid monoclonal antibody-based direct immunofluorescence (IF) test for respiratory syncytial virus (RSV) antigen in nasopharyngeal aspirates (NPAs) proved sensitive and specific, and dual virus infections were very rare when the test was used during the winter RSV season. We therefore recommended that virus isolation in cell culture be restricted to RSV antigen-negative specimens when RSV infection was highly prevalent (October to March in the UK). We reviewed the effect of this policy on the detection of RSV and other viruses in NPAs. The number of NPAs received each RSV epidemic increased markedly with time (3804 in 1984–88, 4569 in 1989–93, <em>P</em> < 0.0001), while during the test of the year the numbers declined slightly (753 vs. 700 for the same periods, <em>P</em> < 0.05). The RSV positivity rate increased October to March (1447/3588 (40%) in 1984–89, 2357/4591 (48%) in 1989–94, <em>P</em> < 0.0001). Restricted use of virus isolation led to a dramatic decline in the yield of RSV in cell culture from RSV IF-negative NPAs (196/3804 (5.2%) for 1984–88, 30/4569 (0.66%) for 1989–93, <em>P</em> < 0.0001). The already low yield on non-RSV respiratory viruses in cell culture was not affected by this policy. A strategy is proposed for the detection of RSV and other respiratory viruses in NPAs which may make virus isolation on such specimens obsolete.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"8 2","pages":"Pages 93-98"},"PeriodicalIF":0.0,"publicationDate":"1996-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0888-0786(96)80006-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81106867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differences in antigenic profile between natural and recombinant HIV-1 p24 identified with monoclonal and polyclonal antibodies 用单克隆和多克隆抗体鉴定天然和重组HIV-1 p24抗原谱的差异

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1996-05-01 DOI: 10.1016/S0888-0786(96)80002-3

Carlos A. Duarte , Ana Elena López , Jesús Benítez

引用次数: 0

Does Aspergillus fumigatus play a role in the disease progression from HIV to AIDS 烟曲霉在从HIV到AIDS的疾病进展中起作用吗

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1996-02-01 DOI: 10.1016/S0888-0786(96)80023-0

P. Bhatnagar, D. Chattopadhya, P. U. Sarma

引用次数: 1