Serodiagnosis and Immunotherapy in Infectious Disease最新文献_第2页

The value of ELISA vs. negative Coombs findings in the serodiagnosis of human brucellosis ELISA与阴性Coombs结果在人布鲁氏菌病血清诊断中的价值

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1997-01-01 DOI: 10.1016/S0888-0786(96)01073-6

G.F. Araj , G.N. Awar

{"title":"The value of ELISA vs. negative Coombs findings in the serodiagnosis of human brucellosis","authors":"G.F. Araj , G.N. Awar","doi":"10.1016/S0888-0786(96)01073-6","DOIUrl":"10.1016/S0888-0786(96)01073-6","url":null,"abstract":"<div><p>This study was conducted to assess the value of ELISA findings in relation to negative findings in the <em>Brucella</em>-antihuman globulin (Coombs) test and in relation to the clinical condition of patients. One hundred and thirty three serum specimens, representing the same number of patients, submitted for serologic testing for brucellosis and showing negative Coombs, were tested by ELISA to determine their <em>Brucella</em>-IgG, -IgM and -IgA antibodies. Concordant negative results between Coombs and ELISA were found in 95 (71.4%) patients whose medical records also did not reveal suggestive clinical signs or symptoms of brucellosis. The elevated ELISA readings in the remaining 38 (28.6%) patients were distributed as follows: IgG + IgM + IgA in 1 patient, IgG + IgM in 8 patients, IgG alone in 24 patients and IgM alone in 5 patients. The clinical review of these patients indicated no current disease in 21 (15.8%), scanty evidence of brucellosis in 8 (6.0%) and suggestive or sufficient evidence in 9 (6.8%). Thus, ELISA is the test of choice to resort to in the case of clinical suspicion of brucellosis, even when the Coombs test shows negative findings.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"8 3","pages":"Pages 169-172"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0888-0786(96)01073-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74145235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Comparative evaluation of eight commercial tests for the diagnosis of infectious mononucleosis by Epstein-Barr virus-specific or -non specific serology eb病毒特异性或非特异性血清学诊断传染性单核细胞增多症的8种商用检测方法的比较评价

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1997-01-01 DOI: 10.1016/S0888-0786(96)01063-3

B. Pozzetto, A.D. Mbida, T. Bourlet, F. Grattard, L. Bonnevial

{"title":"Comparative evaluation of eight commercial tests for the diagnosis of infectious mononucleosis by Epstein-Barr virus-specific or -non specific serology","authors":"B. Pozzetto, A.D. Mbida, T. Bourlet, F. Grattard, L. Bonnevial","doi":"10.1016/S0888-0786(96)01063-3","DOIUrl":"10.1016/S0888-0786(96)01063-3","url":null,"abstract":"<div><p>Eight commercially-available tests were compared for the diagnosis of Epstein-Barr virus (EBV)-associated infectious mononucleosis (IM). They consisted in four slide agglutination assays (one of them using horse erythrocytes and three of them latex beads coated with purified bovine erythrocyte antigen), in a classical Paul-Bunnell-Davidsohn (PBD) tube-test using sheep erythrocytes, and in three assays intended to detect EBV-specific IgM antibodies either by indirect immunofluourescence (one test) or by ELISA (two tests). Sixty serum specimens from patients with symptoms compatible with IM were selected on the following criteria: presence of EBV-specific IgM by at least 2 of the 3 specific assays and absence or very low titres (≤5) of antibodies to EBV nuclear antigen (EBNA). Using this panel of positive samples, the sensitivity of the 8 assays ranged from 55 to 100%. The EBV-specific IgM tests exhibited the higher sensitivities, especially the one using a combination of peptides directed to different viral antigens. Whereas the PBD tuve test was shown to be time-consuming and insensitive, even in young adults, the slide agglutination tests were easy to perform and exhibited sensitivities ranging from 70 to 80%. The latter tests represent a good alternative to EBV-specific serology for the rapid diagnosis of IM; however, in case of negativity, an EBV specific testing must be done, especially in children under 5 years.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"8 3","pages":"Pages 221-223"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0888-0786(96)01063-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76959332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparison of a toxin neutralization assay and ELISA for determination of pertussis toxin antibodies 毒素中和法与ELISA法测定百日咳毒素抗体的比较

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1997-01-01 DOI: 10.1016/S0888-0786(96)01072-4

Jerker Isacson , Birger Trollfors , Teresa Lagergrd , John Taranger

引用次数: 6

Modified ELISA for hydatid serodiagnosis: the potential of periodate treatment and phosphorylcholine inhibition 改良ELISA用于包虫病的血清诊断:高碘酸盐治疗和磷胆碱抑制的潜力

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1997-01-01 DOI: 10.1016/S0888-0786(96)01069-4

Silvia Sterla , Inger Ljungström , Alberto Nieto

引用次数: 14

Detection of toxoplasma-specific antibody in human saliva using conventional assays 用常规方法检测人唾液中弓形虫特异性抗体

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1997-01-01 DOI: 10.1016/S0888-0786(96)01079-7

J. Dave, A. Johnson, N. Andrews, J. Harford, A. Balfour, D. Tompkins

引用次数: 0

The use of recombinant Escherichia coli LamB protein to study the human antibody response to an individual meningococcal PorA outer membrane protein epitope 利用重组大肠杆菌LamB蛋白研究人对单个脑膜炎球菌PorA外膜蛋白表位的抗体反应

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1997-01-01 DOI: 10.1016/S0888-0786(96)01065-7

M.E. Taylor , J. McCarvil , I.M. Feavers , D.M. Jones , A.J. Fox

引用次数: 0

The immune response to a meningococcal 200 kDa surface exposed protein following carriage and disease 携带和患病后对脑膜炎球菌200 kDa表面暴露蛋白的免疫反应

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1997-01-01 DOI: 10.1016/S0888-0786(96)01075-X

R. Borrow, A.J. Fox, D.M. Jones

引用次数: 2

First documented case of human immunodeficiency virus type 2 infection in Turkey 土耳其首例人类免疫缺陷病毒2型感染病例

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1996-05-01 DOI: 10.1016/S0888-0786(96)80012-6

G. Yilmaz , H. Akalin , N. Işik , A.H. Assaf , O. Töre , S. Badur

引用次数: 2

IPAzyme Chlamydia and microimmunofluorescence tests for detecting antibodies against Chlamydia trachomatis in women undergoing laparoscopy IPAzyme衣原体和微免疫荧光试验检测腹腔镜妇女沙眼衣原体抗体

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1996-05-01 DOI: 10.1016/S0888-0786(96)80001-1

R. Zbinden , G. Bon , I. Heinzer , M.F. Paccaud , G. Schär , W. Stoll

{"title":"IPAzyme Chlamydia and microimmunofluorescence tests for detecting antibodies against Chlamydia trachomatis in women undergoing laparoscopy","authors":"R. Zbinden , G. Bon , I. Heinzer , M.F. Paccaud , G. Schär , W. Stoll","doi":"10.1016/S0888-0786(96)80001-1","DOIUrl":"10.1016/S0888-0786(96)80001-1","url":null,"abstract":"<div><p>Superficial <em>Chlamydia trachomatis</em> infections are diagnosed by antigen detection whereas serological investigations are mainly performed to detect deep-seated infections. In this study the genus-specific IPAzyme and two species-specific microimmunofluorescence (MIF) tests were compared for detecting antibodies against <em>C. trachomatis</em> in women undergoing laparoscopy for diagnostic purposes or for ligation of the fallopian tubes. Microbiological findings were similar in both groups. <em>C. trachomatis</em> was detected in 4 of 38 women with ligation and in 6 of 61 women with diagnostic laparoscopy. Serum IgG antibody titres by IPAzyme were clearly positive in 24 out of 61 of the diagnostic group and in 12 out of 38 of the ligation group. However, 14 (39%) of the 36 IPAzyme-positive results were caused by antibodies against <em>Chlamydia pneumoniae</em> and/or <em>Chlamydia psittaci</em>, only 4 (11%) were caused by anti-<em>C. trachomatis</em> IgG and 8 (22%) were caused by both antibodies as tested by a conventional MIF; in 10 all MIF titres were lower than 1:32. A commercial MIF still containing too much genus-specific lipopolysaccharides was more sensitive, but also more crossreactive than the conventional MIF. IPAzyme IgA were only found in association with positive IPAzyme IgG results. We conclude that in case of presumed deep-seated <em>C. trachomatis</em> infection only sensitive species-specific assays for detecting <em>C. trachomatis</em> antibodies are helpful. IPAzyme and other genus-specific assays cannot be recommended to detect antibodies to <em>C. trachomatis</em> in the serum, because positive results are caused mainly by antibodies against respiratory chlamydiae.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"8 2","pages":"Pages 67-71"},"PeriodicalIF":0.0,"publicationDate":"1996-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0888-0786(96)80001-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73653710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative evaluation of commercial methods for the detection of parvovirus B19-specific immunoglobulin M 细小病毒b19特异性免疫球蛋白M检测方法的比较评价

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1996-05-01 DOI: 10.1016/S0888-0786(96)80010-2

Fernando de Ory, María Eulalia Guisasola, Alicia Téllez, Carlos Jorge Domingo

{"title":"Comparative evaluation of commercial methods for the detection of parvovirus B19-specific immunoglobulin M","authors":"Fernando de Ory, María Eulalia Guisasola, Alicia Téllez, Carlos Jorge Domingo","doi":"10.1016/S0888-0786(96)80010-2","DOIUrl":"10.1016/S0888-0786(96)80010-2","url":null,"abstract":"<div><p>The serological diagnosis of parvovirus B19 (PVB19) was performed by radioimmunoassay or enzyme-linked immunosorbent assay (ELISA), using plasma-derived antigen, in view of the difficulties in obtaining the virus. Since the development of recombinant proteins, a number of commercial kits for detecting both specific immunoglobulin G (IgG) and IgM have become available. We evaluated seven methods, including indirect- and <em>μ</em>-chain-capture ELISA, indirect immunofluorescence and Western blot, in an effort to identify PVB19 IgM. In acute samples of erythema infectiosum, the sensitivity ranged from 37% to 91%; in all samples from primary PVB19 infections, including acute and convalescent samples of erythema infectiosum, and from other primary infections in adults, the sensitivity varied from 31% to 79%. When samples from rubella, measles, infectious mononucleosis and healthy pregnant women were tested, specificity ranged from 67% to 100%. Not all the kits evaluated can be used to diagnose PVB19 infections in view of the limitations of some kits with respect to sensitivity and, more importantly, specificity.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"8 2","pages":"Pages 117-120"},"PeriodicalIF":0.0,"publicationDate":"1996-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0888-0786(96)80010-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81227770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7