Serodiagnosis and Immunotherapy in Infectious Disease最新文献

Leptospirosis: a problem for transfusion medicine? 钩端螺旋体病:输血医学的问题?

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1997-01-01 DOI: 10.1016/S0888-0786(96)01076-1

M.A. Ribeiro , M.G. Cliquet , M.G.S. Santos

{"title":"Leptospirosis: a problem for transfusion medicine?","authors":"M.A. Ribeiro , M.G. Cliquet , M.G.S. Santos","doi":"10.1016/S0888-0786(96)01076-1","DOIUrl":"10.1016/S0888-0786(96)01076-1","url":null,"abstract":"<div><p>Leptospirosis is underdiagnosed owing to its non-specific presentation, especially in the early stages of the infection. In the present research, sera from 2368 healthy blood donors of a local blood bank were screened for the presence of leptospiral antibodies using IgM-Pk-ELISA, followed by the microscopic and macroscopic agglutination tests. Serum samples showing two positive and/or borderline results in these assays were confirmed by immunoblotting. The IgM-antibody profile was defined according to the following criteria: sera reactive to a diffuse band of molecular weight 14.8-22 kDa were considered leptospirosis positive, sera exhibiting no reactivity to this band were considered negative. Our results showed a prevalence of 1.01% positivity. There is evidence that transmission of <em>Leptospira interrogans</em> by blood transfusion is possible, but, to date there has been no documentation of transfusion-transmitted leptospirosis. Thus, according to the currently available information, there is no need for new bloodbanking procedures to identify possible carriers of <em>L. interrogans</em>.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"8 3","pages":"Pages 185-189"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0888-0786(96)01076-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79945560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Immunoreactive molecules of Brugia malayi and their diagnostic potential 马来布鲁氏菌的免疫反应分子及其诊断潜力

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1997-01-01 DOI: 10.1016/S0888-0786(96)01081-5

Usha Singh , S. Misra , P.K. Murthy , J.C. Katiyar , A. Agrawal , A.R. Sircar

{"title":"Immunoreactive molecules of Brugia malayi and their diagnostic potential","authors":"Usha Singh , S. Misra , P.K. Murthy , J.C. Katiyar , A. Agrawal , A.R. Sircar","doi":"10.1016/S0888-0786(96)01081-5","DOIUrl":"10.1016/S0888-0786(96)01081-5","url":null,"abstract":"<div><p>Antigen derived from three major life-stages of human lymphatic filariid, <em>Brugia malayi</em> was fractionated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and reactivity of filarial antibodies, present in sera of human bancroftian patients belonging to different categories of the disease, to immunoreactive molecules was evaluated by Western blotting and immune recognition. Antigen molecules of >180, ~180, ~116, ~66, 58, 33 and <20 kDa were strongly identified in blots especially by symptomatic and endemic normal sera. Although mf positive serum reacted with the majority of these molecules, the intensity of bands was poor. These functional molecules along with ~43 kDa were later isolated from a preparative SDS-polyacrylamide gel by elution. The diagnostic utility of these purified molecules was then assessed by enzyme linked immunosorbent assay (ELISA) for detecting the IgG antibodies in patients' sera of various categories. Tropical pulmonary eosinophilic subjects revealed highest reactivity with <20 and ~116 kDa molecules while mf positive ones reacted feebly with the majority of the molecules except ~43 and ~66 kDa. Sera of endemic normals revealed high IgG levels but antibodies to ~ 66 kDa were highest. Symptomatic patients showed moderate reactivity. Non-endemic sera neither reacted in blots nor in ELISA. The study demonstrates the usefulness of ~43 kDa in detecting IgG antibodies of mf positive asymptomatic patients. High IgG levels to ~66 kDa followed by ~116 kDa and <20 kDa in sera of endemic normals warrants their immunoprophylactic evaluation.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"8 3","pages":"Pages 207-212"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0888-0786(96)01081-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80015686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Hepatitis E E型肝炎

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1997-01-01 DOI: 10.1016/S0888-0786(96)01077-3

S. Skidmore

引用次数: 0

A comparison of VDRL and immunoassays developed with a recombinant TmpA antigen in the screening of antibodies to Treponema pallidum 用重组TmpA抗原开发的VDRL和免疫测定法筛选梅毒螺旋体抗体的比较

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1997-01-01 DOI: 10.1016/S0888-0786(96)01070-0

Alina Miranda , Jesus Benítez , Boris Acevedo , Maria del C. Domínguez , Adelaida Villareal , Daniel Palenzuela , Lilian Rodriguez , José L. Rodriguez , Zoe Núñez , Jorge V. Gavilondo

{"title":"A comparison of VDRL and immunoassays developed with a recombinant TmpA antigen in the screening of antibodies to Treponema pallidum","authors":"Alina Miranda , Jesus Benítez , Boris Acevedo , Maria del C. Domínguez , Adelaida Villareal , Daniel Palenzuela , Lilian Rodriguez , José L. Rodriguez , Zoe Núñez , Jorge V. Gavilondo","doi":"10.1016/S0888-0786(96)01070-0","DOIUrl":"10.1016/S0888-0786(96)01070-0","url":null,"abstract":"<div><p>Using the polymerase chain reaction (PCR), and DNA extracted from a lesion of a syphilis patient, we isolated the gene encoding for the mature <em>Treponema pallidum</em> TmpA membrane protein, and expressed this antigen in the cytoplasm of <em>E. coli</em> as a soluble fusion protein. The antigen, purified in one step by immobilized metal ion affinity chromatography (IMAC), was used to develop a visual immunoassay and an ELISA. When these systems were compared with the non-treponemal Venereal Disease Research Laboratories (VDRL) test we found very low discrepancy (0.9% and 0.83%, respectively) in a study of more than 1000 samples. The reactivity pattern of our sera panel in the TmpA visual and ELISA tests indicated that anti-TmpA antibodies are elicited and/or disappear concomitantly with anticardiolipin antibodies. Finally, we obtained a good correlation between the TmpA assays and the <em>Treponema pallidum</em> Haemogglutination Assay (TPHA) in classifying VDRL weakly reactive samples, suggesting their potential use in the confirmation algorithm of syphilis.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"8 3","pages":"Pages 149-155"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0888-0786(96)01070-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88267383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Immunodiagnosis of human neurocysticercosis: use of heterologous antigenic particles (Cysticercus longicollis) in indirect immunofluorescence test 人神经囊虫病的免疫诊断:利用异源抗原颗粒(长尾囊虫)进行间接免疫荧光试验

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1997-01-01 DOI: 10.1016/S0888-0786(96)01071-2

Adelaide J. Vaz , P.M. Nakamura , C.C. Barreto , A.W. Ferreira , J.A. Livramento , A.B.B. Machado

引用次数: 9

Prospective evaluation of the macroagglutination slide test for Leptospira 钩端螺旋体大凝集玻片试验的前瞻性评价

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1997-01-01 DOI: 10.1016/S0888-0786(96)01078-5

Ignacio Marín-León , Maria José Pérez-Lozano , Ernesto de Villar-Conde , Carmen Dastis-Bendala , Julio Vargas-Romero , Tomás Pumarola-Suñé

引用次数: 1

Comparative evaluation of fibre optic immunosensor with ELISA and IFAT for serodiagnosis of Indian Kala azar 光纤免疫传感器与ELISA和IFAT诊断印度黑热病的比较评价

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1997-01-01 DOI: 10.1016/S0888-0786(96)01080-3

Nidhi Nath, Seema R. Jain, Sneh Anand

引用次数: 0

Serum opsonic and bactericidal activity after Neisseria meningitidis group A + C and Haemophilus influenzae type b vaccination: a comparison of three functional assays 接种A + C组脑膜炎奈瑟菌和b型流感嗜血杆菌后血清抑菌和杀菌活性:三种功能测定的比较

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1997-01-01 DOI: 10.1016/S0888-0786(96)01082-7

S. Hugosson , H. Fredlund , H. Käyhty , P. Olcén

{"title":"Serum opsonic and bactericidal activity after Neisseria meningitidis group A + C and Haemophilus influenzae type b vaccination: a comparison of three functional assays","authors":"S. Hugosson , H. Fredlund , H. Käyhty , P. Olcén","doi":"10.1016/S0888-0786(96)01082-7","DOIUrl":"10.1016/S0888-0786(96)01082-7","url":null,"abstract":"<div><p>Three functional assays on immunity, serum bactericidal activity (SBA), phagocytic killing (PK) and an index calculation of chemiluminescence induced in polymorphonuclear leukocytes (CL-index), were compared with an enzyme linked immunoassay (ELISA) of the concentration of the total Ig-fraction of anticapsular antibodies in 28 healthy adults immunized against <em>Haemophilus influenzae</em> type b (Hib) or <em>Neisseria meningitidis</em> serogroup A and C (Me A + C). The immunization evoked a serum response measurable with all assays and a high concentration of antibodies against McA, McC and Hib usually was associated with functional activity and vice versa. However, qualitative differences were found and five of the vaccines (18%) were non-responders in functional assays despite a significant seroconversion measured by ELISA. A functional test may prove valuable in bacterial vaccine trials as complementary to immunochemical calculations of anticapsular antibodies. For estimates of opsonic activity bactericidal serum activity interferes in the PK assay, which is demonstrated in the present study. As an alternative, the CL assay, with the index technique described, is a reliable, less laborious and effective test on opsonophagocytosis.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"8 3","pages":"Pages 213-220"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0888-0786(96)01082-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77271428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Four-year prospective evaluation of immediate early nuclear antigen detection in cell culture for rapid diagnosis of human cytomegalovirus infection 细胞培养中即时早期核抗原检测快速诊断巨细胞病毒感染的四年前瞻性评价

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1997-01-01 DOI: 10.1016/S0888-0786(96)01074-8

David J. Morris , Mohammed O.A.-R. Qutub , A. David Semple

引用次数: 0

Comparison of a commercial ELISA kit and a neutralization assay for assessment of humoral immunity to measles virus 商用酶联免疫吸附测定试剂盒和中和试验评估麻疹病毒体液免疫的比较

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1997-01-01 DOI: 10.1016/S0888-0786(96)01064-5

Maureen G. Friedman , Svetlana Romanova , Aharon Galil , Batia Sarov , Ron Dagan

引用次数: 3